JP2007204423A - Method for producing extract of bamboo grass and use of the extract - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a method for producing a bamboo grass extract capable of efficiently extracting a specific component and provide a physiologically active composition containing the extract. <P>SOLUTION: The bamboo grass extract is produced by steaming the leaves and/or culms of bamboo grass with dry steam having a dryness improved by a pressure-reducing valve and a vapor-liquid separator at 160-190°C for 30 sec to 1 hr and extracting the steamed product with an aqueous solvent. The invention further provides a physiologically active composition containing the extract and a cosmetic and a food containing the composition. <P>COPYRIGHT: (C)2007,JPO&INPIT

Description

The present invention relates to a Sasa extract obtained by a method for producing a Sasa extract, in which specific components are efficiently extracted by steaming persimmon leaves and / or persimmons with steam having improved dryness.
Specifically, the present invention relates to a physiologically active composition such as an antiallergic agent, skin protective agent, intestinal environment improving agent, liver function improving agent, immunostimulant, antimutagenic agent, antibacterial agent and the like containing Sasa extract as an active ingredient. Furthermore, it is related with the cosmetics and food containing these.

Sasa has long been recognized for its medicinal effects, and Sasa extract has also been reported to have many pharmacological actions such as anti-inflammatory / anti-ulcer, antihypertensive and anti-tumor (Non-patent Documents 1 and 2, etc.).
As an active ingredient in Sasa extract, the antitumor property of soluble hemicellulose has attracted attention, and among them, polysaccharides centered on xylooligosaccharide are considered to play an important role in the expression of antitumor effect. Patent Documents 1 and 2 disclose a method for efficiently extracting saccharides by subjecting Sasa leaves and / or straw to high-temperature, high-pressure saturated steam treatment. Patent Document 3 uses a Sasa extract obtained by high-temperature and high-pressure saturated steam treatment as an antibacterial and antibacterial agent against Helicobacter pylori.
None of the above-mentioned known documents mentions any components other than saccharides, and the steam to be steamed does not go through means for improving the dryness, resulting in a difference from the extract of the present invention. It is likely that it was. In addition, if the saccharide content is higher than necessary, the growth of fungi is promoted and it is not suitable as an antibacterial agent, and it is generally difficult to develop applications from the viewpoint of quality control.

  As disclosed in Patent Document 4, the extraction method from Sasa is roughly divided into hot water and alcohol. What is extracted with ethanol is used as a cosmetic raw material as described in the Pharmaceutical Affairs No. 287 of Non-Patent Document 3 and the cosmetic raw material standards, and its main component is β-amylin of triterpenes and Friedelin. And its medicinal properties are mainly anti-inflammatory.

  On the other hand, conventionally, agents having an intestinal regulating action such as oligosaccharides, bifidobacteria and lactic acid bacteria have been used as intestinal metabolism improving agents. Similarly, foods containing oligosaccharides, bifidobacteria and lactic acid bacteria are also used as foods for improving intestinal metabolism.

  In addition, these intestinal bacteria are involved in the metabolism of various substances and endogenous substances derived from food and drugs, and are important for human or animal nutrition, physiology, infectious immunity, carcinogenesis, aging, drug efficacy, etc. It plays a role (Tomochitsu Mitsuoka “The World of Enterobacteria”, Sobunsha, 1980; DJ Hentges “Human Integral Microflora in Health and Disease” Academic Press, 1983).

  In recent years, it has become clear that the balance of these intestinal bacteria is closely related to various immune system diseases. It has been found that it is caused by the disruption of the Th1 / Th2 balance of immune system T cells. Therefore, various studies have been made in order to properly adjust the Th1 / Th2 balance, and attempts have been made to improve the disease by ingesting lactic acid bacteria and increasing useful bacteria such as lactic acid bacteria in the entire intestinal bacteria. Among the diseases caused by Th1 / Th2 imbalance, Th1 dominant amelioration is cedar pollinosis, atopic dermatitis, allergic diseases such as asthma, cancer, chronic fatigue syndrome, ulcerative colitis, candidiasis Chronic hepatitis C, infections caused by bacteria and viruses, and the like.

It has been known that the normal intestinal microflora can be used to cure these diseases with Th1 dominance. However, omega-3 oil (EPA, DHA), olive oil, vitamin A can be used as an improvement method.・ Ingestion of lactic acid bacteria, bifidobacteria, and zinc is known.
Non-Patent Document 4 describes that the number of enteric harmful bacteria is specifically reduced by ingesting 1.2 g of tea polyphenols, which are tannins, per day.

It is also known that when harmful bacteria prevail in the intestine, harmful substances such as ammonia, amines and hydrogen sulfide are generated due to decay and are absorbed into the body to cause rough skin. The skin epidermis has a protective function against external stimuli, and the reduction of the barrier function causes rough skin, and is also a cause of exacerbating various skin diseases such as atopic dermatitis.
In addition, in order to obtain an external preparation for skin having prevention and improvement of skin aging, anti-inflammation, and wound healing, a component having a fibroblast activation action or a growth promoting action, a collagen, hyaluronic acid production promoter, etc. Search and formulation have been attempted. For example, loquat extract (Patent Document 5) is disclosed as a fibroblast activation or growth promoter, and yeast extract (Patent Document 6) is disclosed as a hyaluronic acid production promoter.

  Living organisms are inherently exposed to the invasion of viruses, microorganisms, and the like from the outside, and live in situations where foreign self-substances (eg, cancer, self-dead cells, etc.) appear inside. We can survive in this situation because we have a mechanism to eliminate such foreign substances and defend ourselves. Therefore, such deterioration and abnormality of the body defense ability (immunity ability) may cause various diseases such as bronchial asthma, allergic diseases, chemical sensitivity, rheumatoid arthritis, autoimmune diseases, chronic fatigue syndrome, viruses and bacteria. become. Generally, stress, infectious diseases (measles, influenza, AIDS, etc.), chemical exposure, etc. are considered to be the factors that lead to such a decline in immunity, and they decrease with age.

  The in vivo immune system plays an important role in the defense against tumors and infections by microorganisms such as bacteria, yeasts, molds and viruses, and the main mechanism is that T lymphocytes and B lymphocytes are these microorganisms. Or stimulating tumors by recognizing them through the antigen receptor, activating antigen-specifically and enhancing their ability to eliminate these foreign substances. In a living body that is constantly exposed to microorganisms and whose cells are mutated, such activation of T lymphocytes and B lymphocytes always occurs. In addition to antigen-specific activation, activation of T lymphocytes and B lymphocytes includes non-antigen-specific activation via receptors that recognize bacterial components, lectins, and the like. In both antigen-specific and non-antigen-specific activation, the activation of T lymphocytes and B lymphocytes is further increased when a stimulus from the other, ie, costimulation is applied. Current immunostimulators enhance the body's defense mechanisms against microbial infection and tumors by non-specifically activating immunocompetent cells, but few are satisfactory in effectiveness, and these Since an immunostimulant alone activates immunocompetent cells, it induces an immune response that is undesirable for the living body, and causes side effects such as exacerbation of various allergic reactions.

On the other hand, administration of drugs such as various antibiotics is carried out as a coping therapy for diseases affected by decreased immunity, for example, infectious diseases. However, problems such as the emergence of drug-resistant bacteria have arisen as a result of the easy use of such drugs. From these reflections, searching for substances that can maintain health by improving the immunity of the living body has been widely conducted.

  In addition, the search for substances that act on the genetic material DNA of cells to damage and cause mutations and suppress mutagens that cause cancer has been widely conducted, and various antimutagenic agents have been found. Antimutagenic agents are expected to prevent DNA damage, have anticancer effects, and are widely used to prevent cellular aging. Ascorbic acid having a reducing action is widely known as an antimutagenic agent (Non-patent Document 4). Searches from plants are also widely conducted, and it is known that polyphenols, which are plant components, also have an antimutagenic effect using the Ames test using Salmonella.

  On the other hand, in our eating habits, what has been seen as a problem in health management in recent years is the effect of active oxygen generated in the living body, which seems to be due to various synthetic food additives and residual agricultural chemicals. Living organisms work to remove this harmful active oxygen by enzymes, etc., but when this removal function decreases due to aging etc., even normal cells and organs may be damaged, resulting in various diseases and There are concerns about aging of the organization. For these reasons, the usefulness of foods containing a large amount of components having an antioxidant action, such as vitamin E and vitamin C, has been pointed out.

Recently, interest in adult diseases, in particular, cancer, stroke, heart disease, etc. has been greatly increased, and daily eating habits are being reviewed. For example, high blood pressure is regarded as a risk factor for cardiovascular diseases, and it is said that hypertensives are 2 to 3 times more likely to have a stroke or heart disease than normal blood pressure. In addition, obesity in middle-aged people and the accompanying increase in neutral fat and cholesterol are at high risk of causing arteriosclerosis and myocardial infarction. Avoid excessive drinking, irritating seasonings, high-fat foods, etc. as much as possible and have a good balance. It is said that it is important to keep a meal.

Furthermore, there is a high level of interest because air pollution and ozone layer destruction, which are identified as familiar environmental problems, can cause health problems. In particular, the destruction of the ozone layer increases harmful ultraviolet rays and causes serious problems such as spots, freckles, and skin cancer. For this reason, some regions encourage clothing or sunglasses that do not expose the skin as much as possible and call for protection.

  On the other hand, especially recently, it has become increasingly clear that free radicals cause various diseases (Non-Patent Document 5), and the relationship with aging, carcinogenesis, and anti-cancer has become quite clear. Yes. In recent years, air pollution has been cited as one of the reasons why the prevalence of allergic diseases in the population has increased. For example, in the case of automobile exhaust, black soot in it emits a large amount of active oxygen, It has recently been found that this causes allergic symptoms. If active oxygen is the direct cause of allergic disease, an active oxygen scavenger may be administered, but the relationship between patients with allergic disease and foods and drinks that have active oxygen scavenging activity will be studied over a long period of time. As a matter of course, there are few papers that have been made.

  Under such circumstances, it has become necessary to prevent various diseases in a preventive manner, and the development of various functional materials, and functional foods and beverages and cosmetics are becoming increasingly important. It is coming.

Japanese Unexamined Patent Publication No. 06-197800 Japanese Unexamined Patent Publication No. 11-199502 JP 2002-322079 A JP 2001-321119 Japanese Patent Publication No.5-17206 JP-A-8-163983 J. Hokkaido For. Prod. Res. Inst. Vol. 9, No. 6, 1995 Showa Medical Society Volume 48, No. 5, 595-600, 1988 Pharmaceutical Affairs No. 287 (http://www.nihs.go.jp/mhlw/tuchi/1998/980324c/980324c.html) Biosci.Biotech.Biochem., Vol.46, 588-591 (1992) Osawa, T. et al .: Biochem, Biophysic, Res. Commun., 95; 835, 1980 Pharmacia Vol.29, No.9, p971-1002, published in 1993

  The object of the present invention is to provide a production method for efficiently extracting a specific component that has not been focused as a component of the Sasa extract until now, and a Sasa extract containing a large amount of the specific component, An object of the present invention is to provide a highly safe and excellent bioactive composition having a wide range of pharmacological actions such as intestinal environment improvement action, liver function improvement, immunostimulatory action, antimutagenic action, and antibacterial action. Furthermore, it is providing the cosmetics and food containing these.

In the present invention, bamboo leaves and / or straw are steamed at 160-190 ° C. for 30 seconds to 1 hour with dry steam whose dryness is improved by a pressure reducing valve and a gas-liquid separator, and then extracted with an aqueous solvent. It is related with the Sasa extract characterized by being obtained by the manufacturing method of the Sasa extract characterized by these.
Further, physiological activities such as an antiallergic agent, skin protective agent, intestinal environment improving agent, liver function improving agent, immunostimulatory agent, antimutagenic agent, etc., characterized in that it preferably contains 0.001 to 50% by weight. Relates to the composition.
Furthermore, it is related with the cosmetics and food containing these.

  The Sasa extract of the present invention has a high content of specific components such as phenylpropanoids such as acetic acid, formic acid, coumaric acid, ferulic acid, caffeic acid, and vanillin, and 3-hydroxypyridine, and suppressed the content of saccharides. Is. In addition, the bioactive composition containing Sasa extract as an active ingredient is not only an immunostimulatory effect, but also an antiallergic agent, skin protectant, antimutagenic effect, antibacterial effect, antioxidant effect, anticancer effect, intestinal environment It has effects such as improving action, liver function improving action, neutral fat lowering action, antihyperglycemic action, antihypertension, and metabolic improvement.

Due to overwork, stress, aging-related physiological aging, unbalanced diets, environmental changes, etc., our bodies tend to lose their ability to maintain homeostasis or become unbalanced. The physiologically active composition of the present invention is expected to have an effect of enhancing and improving such functions or adjusting the balance to improve a healthy constitution that is unlikely to cause illness by being used as a food or drink or cosmetic. it can.
Ingested especially as food, it helps increase the number of useful microorganisms in the intestines and contributes to health maintenance. In addition, the increase in useful microorganisms suppresses the growth of harmful microorganisms, and ammonia and sulfur produced by harmful microorganisms. Since the generation of malodorous substances such as hydrogen and amine is also suppressed, malodors such as manure can be improved.

  The spider used in the present invention preferably belongs to the genus Kumazasa, for example, Gotenbazasa, Katsuragazasa, Suzutake, Miyama Kumazasa, Nemagaridake, Miyakozasa, Hosobazasa, Kumazasa, Kumazasa, Chugokusasa, and most preferably Kumiza. In addition, it is well known that the components accumulated in the leaves and persimmons vary depending on the season, and the persimmons harvested from July to September are optimal for use in the present invention.

  The dried bamboo leaves and / or straw used in the steaming of the present invention are preferably dried, and the preferred moisture is 10% or less with respect to the whole bamboo leaves and / or straw, and if it is more than that, spoilage, fermentation Due to this, the components tend to be altered. As for the form of the bamboo leaf and / or the bamboo shoot used in the steaming of the present invention, a chopped piece is preferable, and a preferable size is 0.5 to 20 mm. If it is smaller than 0.5 mm, solid-liquid separation after extraction becomes difficult, and if it is larger than 20 mm, the extraction efficiency tends to decrease.

  In the present invention, steaming of persimmon leaves and / or persimmons can be carried out by any apparatus that can withstand high temperatures and high pressures, and the temperature during steaming is most preferably 160 to 190 ° C. If the temperature is lower than 160 ° C, the cell wall is hardly destroyed by the decomposition of hemicellulose in the cocoon, so that the desired component cannot be sufficiently extracted. is there. The cooking time is preferably 30 seconds to 1 hour, more preferably 5 minutes to 20 minutes. When the time is shorter than 30 seconds, the hydrolysis of hemicellulose is insufficient and the amount of the component is reduced. When the time is longer than 1 hour, the obtained component is decomposed and the amount of the obtained component is reduced. When releasing the pressurization after completion of cooking, it is preferable to open the apparatus at once to bring the pressure to atmospheric pressure, since the destruction of the tissue of the sasa leaves or straw will progress further and the extraction efficiency will improve.

The steam used for steaming is most preferably steam of an apparatus provided with a gas-liquid separation device and a pressure reducing valve in the middle of a steam pipe connecting a steam generating source such as a boiler. By using the apparatus, the dryness of the steam is improved. As the degree of dryness is high and the amount of condensate of steam called drain is small, it is possible to efficiently extract a specific component and appropriately control the extraction amount of sugars. Since there is no simple measurement method for the dryness of the vapor, it is difficult to directly measure it with an ordinary manufacturing apparatus. Therefore, in order to obtain dry steam, it is preferable to use a gas-liquid separator that has been confirmed to be able to achieve a dryness of 99% or more by a dryness measuring system owned by a gas-liquid separator manufacturer or the like. There are a centrifugal type and a baffle type as the gas-liquid separator, but the baffle type is preferable because it can stably achieve a high dryness regardless of the amount of steam.

  If air remains in the cooking apparatus during cooking, contact between dry steam and Sasa leaves or straw is hindered, resulting in insufficient cooking, leading to a decrease in extraction efficiency. As a means for avoiding the remaining of the air, it is only necessary to introduce steam with the manual or automatic air vent valve opened and to expel the air from the cooking apparatus. The vapor temperature for expelling air may be 80 to 200 ° C., preferably 100 to 180 ° C., and the time for expelling air is 0.5 to 60 minutes.

  The extraction method using an aqueous solvent is not particularly limited, but after cooking, the aqueous solvent is added while cooling or maintaining the temperature, and steamed Sasa leaves or Soak firewood.

  The aqueous solvent used in the present invention is preferably a water-soluble solvent such as methanol, ethanol, acetone or the like, or an aqueous solution thereof, or water. If permitted by the use of the Sasa extract, the extraction efficiency can be increased by using an alkaline aqueous solution. . The alkaline aqueous solution used in the present invention is preferably sodium hydroxide, potassium hydroxide, sodium carbonate, sodium hydrogen carbonate, ammonium hydrogen carbonate or the like.

  In the present invention, the steaming step and the extraction step may be repeatedly performed without departing from the gist of the present invention. In addition, another process such as a holding process may be provided before and after each process.

  Although not yet elucidated biologically or biochemically, phenylpropanoid, acetic acid, formic acid, 3-hydroxypyridine, and the like contained in the extract of the present invention are more effective than each individual component due to the synergistic effect of each component. It is also presumed that it contributes to the improvement of health by developing antibacterial and antimutagenic properties, increasing the activation of biological substances, and improving the internal environment of living organisms.

  Examples of the phenylpropanoids contained in the Sasa extract of the present invention include coumaric acid, ferulic acid, caffeic acid, vanillin, etc., and the content of coumaric acid is the largest, followed by ferulic acid. The amount of phenylpropanoid necessary to effectively act as an antimutagenic agent is preferably 0.5 mg / g or more in the Sasa extract as the total amount of all phenylpropanoids, and 1 mg of coumaric acid alone. / G or more is preferable because high antibacterial properties and antimutagenic properties can be obtained.

  The content of acetic acid and formic acid contained in the Sasa extract of the present invention is preferably 0.5 mg / g or more, more preferably 5 mg / g or more by formic acid alone, and still more preferably 10 mg / g or more by formic acid alone.

  The detailed synthesis route of 3-hydroxypyridine contained in the Sasa extract of the present invention is unknown, but it is presumed to be a substance produced when steaming bamboo leaves and / or straw, and it is an antibacterial agent or antimutation. The content necessary as a chemical agent is preferably 0.5 mg / g or more. 1 mg / g or more is more preferable, and 2 mg / g or more is still more preferable.

  The saccharide contained in the Sasa extract of the present invention is a monosaccharide and / or polysaccharide composed of xylose, arabinose, glucose, mannose, galactose and the like. The antibacterial, antiviral, antitumor and antimutation effects of the Sasa extract of the present invention when the heating residue is adjusted to 40 to 60% by weight is preferably 50 mg / g or less and more. There is a tendency that pharmacological effects such as virulence and anti-inflammatory are impaired. In particular, an excessive amount of saccharide promotes the growth of fungi, and those in which the fungus has grown are completely unsuitable for normal use as a Sasa extract. The heating residue referred to in the present invention is a so-called solid content, which means the content of the residue obtained by removing low-boiling substances such as water by heating. For example, the amount left by heating in a 100 ° C. oven for 60 minutes is used. It is obtained by dividing by the amount before heating.

  Phenylpropanoid, acetic acid, formic acid and 3-hydroxypyridine can be quantified by liquid chromatography, gas chromatography or the like. Saccharides can be quantified by ion chromatography.

  The concentration of the Sasa extract in the physiologically active composition of the present invention is preferably 0.001 to 50% by weight.

  There are no particular limitations on the form of the Sasa extract of the present invention and the physiologically active composition containing the same, and it may be a solution, a solid, or a mixture with other compounds. In the case of a mixture, it may be a spray-dried, freeze-dried, or a treatment for adding a shaping agent such as dextrin. Further, it may be subjected to a selection process such as filtration, column purification, and solvent washing.

  The physiologically active composition of the present invention can be used as it is, but if necessary, it is used in pharmaceuticals, quasi drugs, cosmetics, bath preparations, foods, beverages and the like shown below. Any of the various components and additives that are suitable for the application can be arbitrarily selected and used in combination so that each product can be easily used.

  Specific examples of the physiologically active composition of the present invention include antiallergic agents, skin protective agents, intestinal environment improving agents, liver function improving agents, immunostimulants, antimutagenic agents, antibacterial agents and the like.

  Use forms of the antiallergic agent, skin protective agent, intestinal environment improving agent, liver function improving agent, immunostimulant, antimutagenic agent, antibacterial agent and the like of the present invention are not particularly limited. Any of solid, liquid, and semi-solid forms may be used, or the form itself or a form contained in cosmetics, food, or the like. Moreover, both oral and parenteral are possible.

  For example, when ingested orally, it can be ingested by adding to food as a food additive. When used as a food additive, the amount added is not particularly limited, and may be appropriately determined according to the type of food. For example, liquid foods such as soft drinks and carbonated drinks, and solid foods such as confectionery and other various foods can be added and used. In addition, an example of an administration method when administered to the human body as food is as follows. Administration can be performed by various methods, for example, oral administration using tablets, capsules, granules, syrups and the like. The oral preparation can be produced according to a normal production method. For example, excipients such as starch, lactose and mannitol, binders such as sodium carboxymethylcellulose and hydroxypropylcellulose, disintegrants such as crystalline cellulose and carboxymethylcellulose calcium, lubricants such as talc and magnesium stearate, light anhydrous It can be produced as a tablet, capsule, granule, etc. by appropriately combining and formulating a fluidity improver such as silicic acid.

  In the case of ingesting parenterally, application to the skin, spraying and the like can be mentioned, which can be used by adding to cosmetics. The addition amount is not particularly limited, and may be appropriately determined according to the type of cosmetic.

  Examples of cosmetics include lotions, emulsions, creams, ointments, shaving mousses, oils, packs, shampoos, rinses, treatments, hair tonics, hair conditioners, hair restorers, permanent liquids, hair dyes, body soaps, etc. Skin care and hair care preparations, or bath preparations (any form such as liquid, powder, granule, and solid may be used).

  Also applicable to sprays, suppositories, preservatives, brushing agents, soaps, sanitary products, ointments, skin adhesive films, scratches, cuts, burns, scratches, inflammation, eczema, pimples, acne, rough skin, etc. Medical products for disinfection or treatment or gauze impregnated with them, masks, bandages, eye care agents, inhalants, mouth washes, gargles, toothpastes, and other medical aids, earplugs, gloves, hats , Medical supplies such as lab coats and eyebands, air purifiers, coolers, vacuum cleaners, ventilation fans, masks and other filters, carpets, floorboards, wallboards, wallpaper and other indoor building materials, equipment, furniture and sundries protective sheets, Protective cover, protective plate, masterbatch, paint, ink, clothes, skin contact, bedding, bandage, gauze, handkerchief, pennant, cloth, etc., wrapping paper, notebook, memo, wet tea Shrewsbury, paper towel or cotton, and the like day paper products such as tissue paper.

  As specific examples of the food of the present invention, processed fishery products such as kamaboko, chikuwa, hanpen, processed meat products such as sausage, ham and wiener, processed agricultural products such as tofu, fried chicken, konjac, western confectionery, Japanese confectionery, bread, cake, Jelly, pudding, snacks, cookies, gum, candy, ramune and other sweets, raw noodles, Chinese noodles, buckwheat, udon noodles, sauces, soy sauce, dressing, mayonnaise, sauce, honey, powdered candy, syrups Spices such as curry powder, mustard powder, pepper powder, jam, marmalade, chocolate spread, pickles, soy sauce, sprinkles, processed vegetables and fruits such as canned and bottled vegetables, fruits, cheese, butter, yogurt, etc. Dairy products, fruit juices, vegetable juices, whey beverages, soft drinks, health teas, medicinal alcoholic beverages, etc. , Tablets for health for the purpose of nutraceutical (nutritional supplements), etc., beverages, food products such health-oriented granulate, etc., animal feed, pet food, etc. as a target.

  For example, additives generally used for granulation, such as excipients, binders, disintegrants, lubricants, talc, and fluidity improvers may be used. For example, starch, lactose, crystalline cellulose such as sodium mannitol carboxymethylcellulose, hydroxypropylcellulose, light anhydrous silicic acid such as carboxymethylcellulose calcium and magnesium stearate, cellulose acetate, chitosan, calcium alginate, calcium alginate, defatted rice sugar, Oil cake, soy flour, wheat flour, silicic anhydride, bran, rice bran flour, calcium carbonate, milk calcium, magnesium oxide, sodium bicarbonate, crystalline cellulose, starch, brewer's yeast, sugar, reduced lactose, vegetable oil, etc. By combining and prescribing, it can be produced in the form of tablets, capsules, granules and the like.

Various fats and oils Avocado oil, almond oil, fennel oil, sesame oil, olive oil, orange oil, orange rafa oil, sesame oil, cacao oil, chamomile oil, carrot oil, cucumber oil, beef fat, beef tallow fatty acid, cucuin nut oil, safflower oil , Soybean oil, camellia oil, corn oil, rapeseed oil, persic oil, castor oil, cottonseed oil, peanut oil, turtle oil, mink oil, egg yolk oil, cocoa butter, palm oil, palm kernel oil, owl, coconut oil, beef tallow, Pork fat, milk fat, or hydrogenated products (hardened oil, etc.) of these fats and oils.
Wax beeswax, carnauba wax, whale wax, lanolin, liquid lanolin, reduced lanolin, hard lanolin, candelilla wax, montan wax, shellac wax, etc.
Mineral oil liquid paraffin, petrolatum, paraffin, ozokelide, ceresin, micro-cristan wax, squalene, squalane, pristane, etc.

Fatty acids: natural fatty acids such as lauric acid, myristic acid, palmitic acid, stearic acid, behenic acid, oleic acid, 12-hydroxystearic acid, undecylenic acid, tall oil, lanolin fatty acid, isononanoic acid, caproic acid, 2-ethylbutanoic acid, Synthetic fatty acids such as isopentanoic acid, 2-methylpentanoic acid, 2-ethylhexanoic acid and isopentanoic acid.
Alcohols: natural alcohols such as ethanol, isopyropanol, lauryl alcohol, cetanol, stearyl alcohol, oleyl alcohol, lanolin alcohol, cholesterol, phytosterol, and synthetic alcohols such as 2-hexyldecanol, isostearyl alcohol, and 2-octyldodecanol.

  Polyhydric alcohols ethylene oxide, ethylene glycol, diethylene glycol, triethylene glycol, ethylene glycol monoethyl ether, ethylene glycol monobutyl ether, diethylene glycol monomethyl ether, diethylene glycol monoethyl ether, polyethylene glycol, propylene oxide, propylene glycol, polypropylene glycol, 1, 3-butylene glycol, glycerin, pentaerythritol, sorbitol, mannitol and the like.

Esters Isopropyl myristate, isopropyl palmitate, butyl stearate, hexyl laurate, myristyl myristate, oleyl oleate, decyl oleate, octyldodecyl myristate, hexyldecyl dimethyloctanoate, cetyl lactate, myristyl lactate, diethyl phthalate , Dibutyl phthalate, lanolin acetate, ethylene glycol monostearate, propylene glycol monostearate, propylene glycol dioleate, etc.
Metal soaps Aluminum stearate, magnesium stearate, zinc stearate, calcium stearate, zinc palmitate, magnesium myristate, zinc laurate, zinc undecylenate, etc.

  Gum, saccharides and water-soluble polymer compounds Arabic gum, benzoin gum, dammar gum, guaiac gum, Irish moss, Karaya gum, tragacanth gum, carob gum, quinseed, agar, casein, lactose, fructose, sucrose and its esters, trehalose and its Derivatives, dextrin, gelatin, pectin, starch, carrageenan, pullulan, carboxymethyl chitin or chitosan, hydroxyalkyl (C2-C4) chitin or chitosan with addition of alkylene (C2-C4) oxide such as ethylene oxide, low molecular chitin or Chitosan, chitosan salt, sulfated chitin or chitosan, phosphorylated chitin or chitosan, alginic acid and its salt, hyaluronic acid and its salt, chondroitin sulfate and its salt, heparin, ethylcellulose, methylcellulose , Carboxymethyl cellulose, carboxyethyl cellulose, sodium carboxyethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, nitrocellulose, crystalline cellulose, polyvinyl alcohol, polyvinyl methyl ether, polyvinyl pyrrolidone, polyvinyl methacrylate, polyacrylate, polyethylene oxide, polypropylene oxide, etc. Polyalkylene oxide or its crosslinked polymer, carboxyvinyl polymer, polyethyleneimine, etc.

  Anionic surfactant (carboxylate, sulfonate, sulfate ester salt, phosphate ester salt) as surfactant, cationic surfactant (amine salt, quaternary ammonium salt), carboxylic acid type amphoteric as amphoteric surfactant Surfactant (amino type, betaine type), sulfate ester type amphoteric surfactant, sulfonic acid type amphoteric surfactant, phosphate ester type amphoteric surfactant, nonionic surfactant (ether type nonionic surfactant, Ether ester type nonionic surfactant, ester type nonionic surfactant, block polymer type nonionic surfactant, nitrogen-containing type nonionic surfactant), other surfactants (natural surfactant, protein hydrolysis) Derivatives, polymeric surfactants, surfactants containing titanium and silicon, fluorocarbon surfactants, etc.).

  Various vitamins Vitamin A group: retinol, retinal (vitamin A1), dehydroretinal (vitamin A2), carotene, lycopene (provitamin A), vitamin B group: thiamine hydrochloride, thiamine sulfate (vitamin B1), riboflavin (vitamin B2), pyridoxine (vitamin B6), cyanocobalamin (vitamin B12), folic acid, nicotinic acids, pantothenic acids, biotins, choline, inositols, vitamin C group: ascorbic acid and its derivatives, vitamin D group: ergocalciferol ( Vitamin D2), cholecalciferol (vitamin D3), dihydrotaxosterol, vitamin E group: tocopherol and its derivatives, tocotrienol, ubiquinones, vitamin K group: phytonadione (vitamin K1), menaquinone (vitamin K2 , Menadione (vitamin K3), such as menadiol (vitamin K4).

  Various amino acids valine, leucine, isoleucine, threonine, methionine, phenylalanine, tryptophan, lysine, glycine, alanine, asparagine, glutamine, serine, cysteine, cystine, tyrosine, proline, hydroxyproline, aspartic acid, glutamic acid, hydroxylysine, arginine, Ornithine, histidine, GABA, and their amino acid derivatives such as sulfates, phosphates, nitrates, citrates, or pyrrolidone carboxylic acids.

Moreover, the various additives derived from a plant or animal-type raw material can also be used together as needed.
These additives are processed in a conventional manner according to the type and form of the product to be added (for example, grinding, milling, washing, hydrolysis, fermentation, purification, pressing, extraction, fractionation, filtration, drying) , Pulverization, granulation, dissolution, sterilization, pH adjustment, deodorization, decolorization, etc., may be arbitrarily selected and combined) and selected from various materials. The solvent used for the extraction may be selected in consideration of the usage of the product to be provided (food, external use, bath use), processing to be performed later, etc. Usually, water, a water-soluble organic solvent (for example, It is desirable to use one or a mixture of two or more selected from ethanol, propylene glycol, 1,3-butylene glycol and the like. However, when it is not preferable to contain an organic solvent depending on the application, only water may be used, or ethanol that can be easily removed after extraction may be used alone or in any mixture with water.

  Examples of plant-derived materials (herbal medicines) include, for example, Ai (blue leaves), Aokazura (Keifu Fuji), Aotsuki-rafuji (wooden candy), Acacia, Akasuguri fruits, Red grapes, Akamegasiwa (red buds), Akane (Atsugi roots), Red gecko (ground yellow), Agaricus, Agi (Atsu), Akinire (Crust), Akebi (Kitsu), Asa (Asako Jin), Asagao (Toshiba), Ashitaba (Tomorrow), Azuki (Red Azuki), Asenyaku (Asen Yaku), Acerola, Anise fruit, Abemaki fruit, Avocado, Ama, Achacha (Amacha), Amadokoro (Tamatake), Amigasayuri (shellfish mother), Almond, Altea, Arnica, Aloe (Amber), Aloe vera, Apricot ), Ansokkou (benzoic incense), Igakozolina (Gold head), Japanese knotweed (tiger cane root), Strawberry, Fig (no flower fruit) or its leaves, Ichibi (winter coconut), Japanese tiger bean (distant), Carob, rice seed, seed coat or seed coat Inondo seed, nettle, ylang-ylang, ginkgo (Ginkgo leaves, Ginkgo), Ibuki-so-sou, fennel (Yuka), Yukira (three ridges), Uguisukagra fruit, turmeric (Usu gold), usbeniaoi, usbenitaachioi, usubasaishin (spicy) ), Crabs (summer hay), Japanese pods or shrimp (salted, isolated, tangy), Japanese ume (plum) or its pulp, vulgaris, walruses, mandarin oranges (skin), estragon, sorghum (enzyme), enoki, ebushigusa (Keiko), Elderberry fruit, Elemi, Enju (槐花, 槐花 米), Ougi (Yellow), Ouren (Yelen), Great-crowned cucumber (Karonin), Otsu ヅ rafuji (Prevention), Psyllium Car front grass), toucan beetle (lion), oberon okera (white birch), Obayayashabushi fruit, large hawthorn (mountain checkered), barley (barley), ogurma (turnover) Okra fruit, Okera (white birch), Panax ginseng (carrot), Hypericum perennial (cutting younger brother), Odrichospodium (continuous), Onamomi (Amber ear), Onigurumi, Oninoyara (Tempa), Onyuri (lily), Oninos, Halibut ), Ominaeshi (defeated soy sauce), Dutch strawberry, Dutch pepper, Dutch parsley, Dutch honeybee, olive, oregano, orange fruit or pericarp, silkworm (ripe yellow), seaweed [green algae, brown algae, red algae], cacao seeds, oyster Or its leaves (bamboo shoots), scallops (fishing potatoes), cassia, gambling berries (gumnuts), gadgets (bamboo shoots), oaks (bamboo shoots, cocoon leaves), cascarillas, cynomolguss (kinsato), valerian roots ), Pumpkin, capockonoki seed, kahoku sansho (蜀椒), cattail (蒲 yellow), chamomile, camillade (weed), crow uri (king moth), oats, crow birch (half-summer), guarana seed , Karaya, Karin (Kiso), Kawayanagi, Kawaratake, Kawaramugi (Kin Chin), Kansui (licorice), Kanzo (licorice), Candelilla, Kanto, Kanna, Raspberry, Kiwi fruit, Kikarasuuri (Gurone) (Chrysanthemum, bellflower root), Chrysanthemum (Chrysanthemum flower), Catalpa (Cultivation), Gimushishi (Yotei), Kizuta, Yellowtail aloe, Yellowtail, Yellowfin, Yellowfin (Yellow red), Yellow-tailed yellow (Yellow yellow), Gymnema Sylvesta, Kiraja Saponaria , Kiraya, cabbage, cabbage immature fruit, cucumber, kumquat fruit, betel, kinzushiki (Sengakurasuki), guava, guayule, quellus infectria (garlic), kukui nut, wolfberry (bamboo shoots, coconut leaves, maple leaves, ground bark) , Kusasugikazura (Tenmon Fuyu), Kudu (Kuzukone), camphor tree, gooseberry fruit, gardenia (Yamako), Kunugi (Kanso), Kumazasa, Ku Tsutsura (horse whip grass), Clara (bitter ginseng), cranberry fruit, chestnut or its astringent skin, curcumrigo latifolia fruit, grapefruit fruit, buckthorn, black-headed beetle (emergency), black-bellied kokushi (prolongation grass), chlorella, mulberry (mulberry leaf, Mulberry white bark), guava fruit, potato oyster (bamboo shoot, chick ear), celosia (chicken flower, hen crown), keirinsaishin (spicy), laurel tree, gentian, gennoshouko (old vine), cilantro fruit, koushinbara (moon season) Flower), Kousei mint, Kouzo fruit, black tea, Kohone (river bone), Kouhon (Tsubakimoto, Karamotomoto), Koryang, Cowberry (Etsutachibana), Koganebana (Yellow Gon), Kogomebaotogiriso, Coconut fruit, Goshuyu (Kurei) , Gosho strawberry (covered bonsai), pepper (pepper), copaiba balsam, coffee beans, kobushi (hot pepper), burdock (beef bowl, beef coconut), kobotanru, go Sesame seeds, sesame seeds (genus ginseng), wheat (wheat), rice or rice bran (red rice cake, white birch), cola acminata seeds, cola vera seeds, fenugreek fruit, colombo, condurango, kombu, comfrey Grass), konjac, saximas buttonzul (wei Sensen), cherry blossoms, leaves, fruits, bark (cherry bark), pomegranate, sasa, sasayuri (lily), sasanqua, sasamodaka (sawabe), sweet potato, sugarcane, sugar beet, Sanebuto jujube (acid soy sauce), saffron (banban flower, west safflower), pomelo fruit, savonso, salmon berry fruit, salamander, salvia, sorghum (mountain curd), sawagurumi leaf, sankirai (mountain return), hawthorn ), Carrot (Ginseng), Sanshuyu (Yamamuro), Salamander (Yamamuro), Sandskon (Yamadone), Cyano fruit, Shiitake mushroom, Diou (geo), On (purple pod), digitalis, shikunshi (ambassador), shishiudo (single life, tang monopoly), perilla, ajizo, chillimenzo, catamendizo (purple leaf, shisoko), weeping birch (white birch bark), rosewood, linden tree, syringae ), Shimakangi (Chrysanthemum flower), Shimotsukeso, Potato, Peonies (Shakuyaku), Shajin (Shasan), Janobige (Bakumon winter), Shirakashi seeds, White crocodile seeds, Shirobanairis, Shirobanatsuta flower, Shirominanten (Southern fruit), Juzudama, palm fruit, ginger (ginger), shobu (bamboo shoot, radish), shozu fruit, birch, cinchona sill bra, cinnamon, watermelon (western phoenix), honeysuckle (gold-silver flower, endurance winter), sorrel (acid model), swim berry Fruit, Strawberry fruit, Horsetail (question), Stevia, Plum fruit, Scots pine cone, Larch, Larch Ivy, walnuts, pear fruit, psyllium serrata, psyllium serrata, psyllium serrata, elderberry, psyllium serrata, scallop, scallop, mistletoe, scorpiona serrata, horseradish, gypsophila serrata , Celery, nematode (river cucumber), senna fruit or leaf or stem, senninsou (daikon), senburi (medicated medicinal), buckwheat (grass), buckwheat seeds, rice potato, daiou (daihuang), radish, soybean, daidai ( Orange peel, fruit), Thyme, Takatodai (Otsuka), Takarabi (Kashiwa spine), Dark sweet cherry fruit, Tachikakuso (Hundred incense), Tachibana (Tachibana peel), Tachiyanagi, Tamarind seeds, Onion, Tamshiba (hot pepper), Taranoki Or its root bark, tanjin, danmar, cheri Fruit, chigaya or its root (sone root), chicory, clove (chome, clove), ginseng (gomi), ginseng (daihuang), ginseng (ginseng), ginseng (ginseng), ginseng (gum leaves), Choreimaitake, camellia, camellia, camellia, clover (lacquered grass), communis (duck grass), tsuzukizuki (red bean), tsurudukudami (what neck rice cake), tsuruna (apricot), tsuruninjin (four leaves ginseng), Azalea, Deco, Teuchigurumi, Duberry fruit, Tengusa, Tendai-yaku (glaze), Toga (winter lion), Pepper (panyu), Toki (Tokio), Toki-Senka, corn or corn hair (Southern cocoon hair), Tonenmumochi ( Sadako), Torindo (Ryuboku), Dokudami (10 medicines), Tokon (Nippon), Tochibaninjin (Takebana Ginseng), Tochu (Tochu, Tonaka), Tomato, Tragacanth, Tomato Liacanthus seeds, Tormentilla, Doronoki, Trolley, Nigerian berry fruit, Chinese yam (Yakuyaku), Nagiikada, Naginatakouju, Nazuna, Natsumikan, jujube (Otsuchi), Naniwaibara (gold cherry), Narukoyuri (peanut), peanut (peanut) , Nanten (Minami Tenmi), Nigaki (Nagiki), Nigamugigi (Anniversary), Nikuzuku, Nikkei (Kinshika), Nira (Etsuko), Elderberry (bone graft), Carrot, Garlic (Otsuchi), Nurde (Pyto), Leek, Noazami (Otsuka), Neubara (fruit), Yarrow, Nodake (Apricot), Novara, Peach, Palm, Hibiscus (Busouge, Furinbusouge, Roselle), Pineapple fruit, Hakatayuri (lily), Hakamurabosi (bone fracture supplement) ), Hakusen (white husk), hakuruberry fruit, chickweed (breeding), hazelnut (coconut), ashridokoro (roth root), basil, lotus (lotus) Renko), Parsley, Batata, Hachiku (bamboo shoots), Patchouli, Pepper (thin load, thin load leaf), pearl barley (Yokuinin), Hanasuge (my mother), bananas, flower mint, vanilla bins, papaya, leaflet (Hakugusa), hub Grass, paprika, clam (branch lion), hamasuge (katsukibushi), kingfish (spotted lion), hamanas (my cocoon flower), kingfisher (hama wind), hamamelis, rose (rose), parietaria, harunire (White skin, maple leaves), breadfruit, Japanese cypress (hibuki), Japanese carrot (participant), pecan nuts, ganbanana (stone wall, vine pearl shahua), Japanese cypress (longevity grass), Japanese cypress (hishin), pistachio, beet, Hitotsuba (stone wall), Hinata Taikozuchi (cow knee), Hiba, Castor, Sunflower, Pepper, Himegama (Koran), Japanese amber mushroom, Pimenta fruit, Ayu fruit, Bellow oyster, Hirohakinagusa (Bald eagle), Hiro Senega (Senega), loquat (bamboo shoots, cocoon leaves), betel nuts (large belly skin, coconut), Japanese quail (Nanto), FUKI (Chou winter flower), FUJI dandelion (Claude winter flower, FUKUBA), Fujibakama (Orchid) (Boiled beans), butcher bloom, grape skin or fruit, beech, fusumina tsutake mushroom (Coleoptera), Brazilian licorice, black currant fruit, blackberry fruit, plum fruit, fluceraria, blueberry fruit, prune, blond psyllium, bundo (green bean) ), Loofah, Safflower (Safflower), Belladonna, Berry fruit, Boysenberry fruit, Bow-fu (Windproof), Spinach, Japanese physalis (Torone), Japanese cypress (Japanese magnolia), Japanese quince (Japanese persimmon), Japanese cypress , Hosobabarengiku, Bodaiju, Button (peony skin), Hop, Jojoba, White berry fruit, Hon apricot, Honshimeji, Mizurutennansho (Tennansei), Maou (mao), macadamia nuts, macri (marine grass), mugwa (mulberry leaves, mulberry bark), mushroom (bamboo moth), matatabi (wooden moth), matsukasa, matsuhod ), Marigold, Malvano Jazine (Apricot Ginseng), Mulberry Fruit, Quince, Maronier, Mango, Mangosteen, Manshugurumi, Mandarin Fruit, Mannentake (Reishi), Mannenrou, Citrus Fruit (Cultivation), Mishima Psycho (Shibako), Mizokakushi (half-sided lotus), Misohagi (Chikuma), Mitsugashi, Mitsuba, Mitsubahamago (Vinegar), Green mint, Mimosa, Myoga, Miracle fruit fruit, Myrrh, Milobaran, Mukage (Mushroom), Mukunoki, Mukuroji, Murasaki (Purple root), purple corn, swordfish (beneficial herb), burdock root, melaroica, melissa, merirot, melon fruit, mou Mushrooms, Mosouchiku, magnolia (powder), mokko (wooden incense), maple leaf peach, peach fruit, seed (peach seed) or leaf (peach leaf), coconut palm, morello cherry fruit, morohaya, yakawamurasakiimo, yakuchi (Michichi) ), Cornflower, cornflower, cornflower mint, yashabushi (arrow wheel), willow willow, starfish (octagonal gold plate), mistletoe (willow infestation), willow leaves, yabgarashi, yabukoji (purple gold beef), pokeweed (commercial land), yamanoimo (mountain) Medicinal), Yamanoki (Yamamuro), Yamamomo (Plum skin), Eucalyptus, Yucca brevifolia, Yukinoshita (Tiger), Yuzu fruit, Lily, Mugwort (Leaf), Lime fruit, Lime fruit, Peanut, Lacanca fruit, Raspberry Fruit, lavender, longan (longan), green tea, apple fruit, lubas suabisimusu (letto), lettuce, red currant fruit, lemon Real, lemon grass, forsythia (Forsythia), Rengesou, Rouyashi, loganberry fruit, rose hips, rosemary, Roman chamomile, seaweed, wasabi, and the like burnet (Chinire).

  Animal-derived raw materials include chicken crown extract, bovine / human placenta extract, porcine / cattle stomach, duodenum or intestinal extract or degradation products thereof, collagen derivatives such as water-soluble collagen, acylated collagen, etc. , Collagen hydrolyzate, elastin, elastin hydrolyzate, water-soluble elastin derivative, keratin and its degradation product, silk protein and its degradation product, porcine and bovine blood cell protein degradation product (globin peptide), porcine and bovine hemoglobin degradation product (Hemin, hematin, heme, protoheme, heme iron, etc.), casein degradation products, chicken eggs, fish meat and their degradation products.

  Other additives include microbial culture metabolite rice fermented extract, rice bran fermented extract, GABA, Euglena extract, lactic acid fermented milk powder, sporic lactic acid bacteria, reduced lactose, indigestible dextrin, polydextrose, guar gum degradation product , Soybean oligosaccharides, fructooligosaccharides, dairy oligosaccharides, galactooligosaccharides, xylo-oligosaccharides, isomalt-oligosaccharides, beet oligosaccharides, maltitol, vegetable oils and fats, α-hydroxy acid glycolic acid, citric acid, malic acid, tartaric acid, lactic acid Inorganic pigments Silicic anhydride, magnesium silicate, talc, kaolin, bentonite, mica, titanium mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, calcium carbonate, magnesium carbonate, yellow iron oxide, bengara , Black iron oxide, Gunjou, Black oxide , Chromium hydroxide, carbon black, calamine, etc. UV absorbers p-aminobenzoic acid derivatives, salicylic acid derivatives, anthranilic acid derivatives, coumarin derivatives, amino acid compounds, benzotriazole derivatives, tetrazole derivatives, imidazoline derivatives, pyrimidine derivatives, dioxane Derivatives, camphor derivatives, furan derivatives, pyrone derivatives, nucleic acid derivatives, allantoin derivatives, nicotinic acid derivatives, vitamin B6 derivatives, oxybenzone, benzophenone, guaiazulene, shikonin, baicalin, baicalein, berberine, etc.

Astringent lactic acid, tartaric acid, succinic acid, citric acid, allantoin, zinc chloride, zinc sulfate, zinc oxide, calamine, zinc p-phenolsulfonate, potassium aluminum sulfate, resorcin, ferric chloride, tannic acid, etc.
Antioxidants ascorbic acid and its salts, stearic acid ester, tocopherol and its ester derivatives, nordihydrogua ceretenoic acid, butylhydroxytoluene (BHT), butylhydroxyanisole (BHA), parahydroxyanisole, propyl gallate, sesamol, Sesamoline, Gossypol, etc.
Anti-inflammatory agents ictamol, indomethacin, kaolin, salicylic acid, sodium salicylate, methyl salicylate, acetylsalicylic acid, diphenhydramine hydrochloride, d or dl-camphor, hydrocortisone, guaiazulene, camazulene, chlorpheniramine maleate, glycyrrhizic acid and its salts, glycyrrhetinic acid and Its salt, etc.
Disinfectant / disinfectant acrinol, sulfur, benzalkonium chloride, benzethonium chloride, methylrosaniline chloride, cresol, calcium gluconate, chlorhexidine gluconate, sulfamine, mercurochrome, lactoferrin or its hydrolyzate, etc.

  Perfumes Natural perfumes such as musk, civet, castorium, ambergris, anise essential oil, angelica essential oil, ylang ylang essential oil, iris essential oil, fennel essential oil, orange essential oil, cananga essential oil, caraway essential oil, cardamom essential oil, guayakwood essential oil, cumin essential oil, Black letter essential oil, cinnamon essential oil, cinnamon essential oil, geranium essential oil, copaiba balsam essential oil, coriandel essential oil, perilla essential oil, cedarwood essential oil, citronella essential oil, jasmine essential oil, gingergrass essential oil, cedar essential oil, spearmint essential oil, western peppermint essential oil , Tuberose essential oil, clove essential oil, orange flower essential oil, winter green essential oil, trout balsum essential oil, buttery essential oil, rose essential oil, palmarosa essential oil, cocoon essential oil, hiba essential oil, sandalwood essential oil, petit gren essential oil, bay essential oil, vetiva essential oil, bergamot essential oil Peruvian balsam essential oil, boad rose essential oil, melamine essential oil, mandarin essential oil, eucalyptus essential oil, lime essential oil, lavender essential oil, linaloe essential oil, lemongrass essential oil, lemon essential oil, rosemary essential oil, Japanese mint essential oil, and other synthetic flavors Such,

Dye / Colorant Red Cabbage Dye, Red Rice Dye, Akane Dye, Anato Dye, Ikumi Dye, Turmeric Dye, Enju Dye, Krill Dye, Amber Dye, Caramel, Gold, Silver, Gardenia Dye, Corn Dye, Onion Dye, Tamarind Dye , Spirulina pigment, buckwheat whole plant pigment, cherry pigment, laver pigment, hibiscus pigment, grape juice pigment, marigold pigment, purple potato pigment, purple yam pigment, lac pigment, rutin, etc.
Sweetener sugar, sweet tea, fructose, arabinose, galactose, xylose, stevia, mannose, erythritol, maltose, honey, glucose, miraculin, monelin, licorice extract, etc.
Nutritional fortifier baked calcium shell, cyanocollamine, yeast, wheat germ, egg yolk powder, hemicellulose, etc.

  Other moisturizers, hormones, sequestering agents, pH adjusters, chelating agents, antiseptic and antibacterial agents, cooling agents, stabilizers, emulsifiers, animal and vegetable proteins and their degradation products, animal and vegetable polysaccharides And its degradation products, animal and plant glycoproteins and their degradation products, blood flow promoters, anti-inflammatory agents, antiallergic agents, cell activators, keratolytic agents, wound treatment agents, foam enhancers, thickeners, oral use Additives, deodorizing / deodorizing agents, bittering agents, seasonings, enzymes, and the like can be mentioned, and in combination with these, additive and synergistic effects can be expected.

  As food, healthy and cosmetic effects such as nutritional supplementation, fatigue recovery, tonicity, cell activation (prevention of cell aging), taste improvement, color and aroma, glossiness, stabilization, thickening, antiseptic, etc. Can be used for purposes. In addition to this, we expect various cosmetic and pharmaceutical effects of each of the raw materials known so far, and by combining these, we aim to enhance the target effect, or are multifunctional It is also possible to make the product expected to be effective.

Hereinafter, the present invention will be described in more detail with reference to examples. In addition,% shows weight%.
(Production Example 1)
2 kg of dried Kumizasa leaves and straws crushed to 0.5 to 5 mm and charged in a high-pressure steam can were charged with 2 MPa of high-pressure steam generated by a once-through boiler with a dryness measurement system of 99. Gas-liquid separation was performed with a separator that was confirmed to be achieved up to 5%, and steam with improved dryness was further introduced into a high-pressure steam can by reducing the pressure to 1.6 MPa with a pressure reducing valve.
At the beginning of steam introduction, the air vent valve is opened and steam is passed through the steaming can at 100 ° C for 10 minutes to expel air, the air vent valve is closed and the temperature is raised from 100 ° C to 180 ° C over 10 minutes. The pressure was released immediately after holding for 10 minutes.
Leave high pressure steamed leaves and straw in a pressure extractor, add 8 kg of water, raise the temperature from room temperature to 110 ° C. over 30 minutes, hold at that temperature for 5 minutes, and then release the pressurized state over 1 hour . The extract was filtered and concentrated under reduced pressure to obtain a Sasa extract with a heating residue of 50%.

(Production Example 2: Dry powder)
An equal amount of dextrin was added to and mixed with the Sasa extract obtained in Production Example 1, and then a dry powder was obtained with a spray dryer.

(Production Comparative Example 1)
With reference to the method described in Example 1 of JP-A-6-197800, a Sasa extract was produced. That is, 20 kg of water was added to 2 kg of dried Kumizasa leaves and straw that had been crushed to 0.5 to 5 mm, and filtered for 3 hours at 80 ° C., followed by filtration. This extraction operation was repeated twice to prepare Kumizasa from which the hot water extract was removed. Next, this was adjusted to a moisture content of 45% and charged into a high-pressure steam can, and the 2 MPa high-pressure steam generated by the once-through boiler was decompressed to 1.6 MPa by a pressure reducing valve and directly introduced into the high-pressure steam can.
The temperature was raised from room temperature to 180 ° C. over 10 minutes, held at that temperature for 10 minutes, and then the pressure was gradually released.
Leave high pressure steamed leaves and straw in a pressure extractor, add 8 kg of water, raise the temperature from room temperature to 110 ° C. over 30 minutes, hold at that temperature for 5 minutes, and then release the pressurized state over 1 hour . The extract was filtered and concentrated under reduced pressure to obtain a Sasa extract with a heating residue of 50%.

(Production Comparative Example 2)
Sama extract with 50% solid content by adding sterilized water to the solid content obtained by filtering and concentrating the kumazaza extract (which is extracted from ethanol with crushed strawberries without steaming process) described in the cosmetic raw material standard, whose main component is triterpenes Got.

<Composition evaluation of Sasa extract>
Composition analysis of the Sasa extract was performed by the following method, and the results are shown in Table 1.
(Composition analysis method)
Qualitative and quantitative analysis of acetic acid, formic acid, coumaric acid, ferulic acid, and 3-hydroxypyridine was performed using a gas chromatograph mass spectrometer equipped with a J & W innowax 30 m × 0.25 mm, 0.25 um column.

  In addition, using DXEX-500 sugar analyzer manufactured by Dionex equipped with CarboPac PA1 as the column and electrochemical detector as the detector, arabinose, rhamnose, galactose, glucose, xylose, mannose, fructose, ribose, xylobiose, xylotriose, xylotetra Qualitative and quantitative analysis of aus, xylopentaose, xylohexaose, arabinobiose, arabinotriose, arabinotetraose, arabinopentaose, arabinohexaose and the total value of all components as sugar content did.

(Antimicrobial test method)
The test bacteria are as follows.
Escherichia coli NBRC 3962
Staphylococcus aureus NBRC12732
Pseudomonas aeruginosa: Pseudomonas aeruginosa NBRC13275
The minimum drug concentration (MIC) at which the Sasa extract diluted at various magnifications was brought into contact with Müller Hinton broth with a substantially constant number of bacteria and the growth of the bacteria was inhibited after a certain period of time was determined and used as an index of antibacterial effect.
The smaller the MIC, the higher the antibacterial effect.

(Anti-mutagenicity test method)
The number of revertant colonies was counted using Salmonella TA100 as the strain and B (a) P; benzo (a) pyrene as the mutagen. As an index of antimutagenicity, a relative value obtained by converting the number of revertant colonies with no Sasa extract sample added to 100 is shown. The smaller the value, the higher the antimutagenicity.

（注１）ササ抽出物未添加を１００とした場合の相対値
（注２）ササ抽出物製造後速やかに菌が繁殖したと考えられ、エキスとしては全く不適当であることを示している。

(Note 1) Relative value when Sasa extract non-addition is 100 (Note 2) It is considered that the bacteria proliferated promptly after the Sasa extract was produced, indicating that it is totally unsuitable as an extract.

In Production Example 1, a Sasa extract in which there were many specific components such as acetic acid, formic acid, coumaric acid, ferulic acid, 3-hydroxypyridine, and the extraction of sugars was suppressed was obtained.
In Production Comparative Example 1, the extraction of specific components was small and a large amount of saccharide was extracted. Bacteria grew on the Sasa extract and could not be used in subsequent experiments.
In Production Comparative Example 2, the extraction amount of each component was generally low and the production efficiency was very poor. Antibacterial properties were also low.

(Example 1, Comparative Example 1: Cream for skin)
According to the formulation in Table 2, a skin cream containing 5% by weight of Sasa extract was produced by a conventional method.

(Test Example 1: Anti-inflammatory / atopic dermatitis)
Atopic dermatitis spontaneously DS / Nh mice 40 weeks 5 weeks were divided into the following 4 groups of 10 mice each.
(1) Non-application group (2) DNFB (2,4-dinitrofluorobenzen (DNFB) acetone / olive oil solution causing contact dermatitis) application group (3) DNFB + Sasa EtOH (DNFB + skin cream of Comparative Example 1) application group,
(4) DNFB + sasa water (DNFB + skin cream of Example 1) application group At the start of the experiment, 1 ml of 0.25% DNFB was applied to a certain part of the skin. Thereafter, the cream was applied 0.75 g / time twice at the same site twice a week and tested until 15 weeks of age.

Anti-inflammatory evaluation was performed by the following method.
1. Skin eruption score (Erythema, wrinkle, dryness was scored in 4 stages, and the average value of the total score was compared. The worse the value, the higher the value.)
2. Serum IgE antibody amount (the amount of IgE antibody in serum was measured using an ELISA kit. It is known that allergy worsens with an increase in IgE antibody.)
3. Cytokine (IL-13) level (after killing the mice, lymph nodes of the neck, groin, and inguinal were removed, and after stimulation with ConA cells, cytokine measurement was performed using an ELISA kit. For cytokines such as IL-13 and IL-4 Increases are known to promote the production of IgE antibodies and lead to exacerbation of allergies.)
The results are shown in Tables 3 and 4 and FIG. (3) In the application group (DNFB + skin cream application group in Comparative Example 1), no improvement is seen compared with (2) application group (DNFB application group), but (4) Application group (DNFB + in Example 1) In the skin cream application group), the skin eruption score, serum IgE antibody level, and IL-13 level were all improved.

1. Skin rash score

2. Serum IgE antibody (μg / ml)

(Example 2, comparative example 2: lotion)
In accordance with the formulation shown in Table 5, a lotion containing 0.1% by weight of Sasa extract was produced by a conventional method.

(Test Example 2: Epidermal cell activation)
A medium was added to the lotions of Example 2 and Comparative Example 2, and epidermal cells were cultured for 24 hours. After culture, the amount of hyaluronic acid in the culture supernatant was quantified by ELISA. At the same time, protein quantification was performed, and the amount of hyaluronic acid per unit protein amount was calculated as the amount of hyaluronic acid produced. Table 6 shows the results.
It was shown that the Sasa extract concentration in the lotion of Example 2 was significant for the activation of epidermal cells when the concentration was 0.01% or more.

１）＊：５％水準で有意差あり
２）濃度はササ抽出物の濃度である
３）P.C.＝陽性対照（100 nM レチノイン酸）

1) *: Significantly different at 5% level 2) Concentration is the concentration of Sasa extract 3) PC = positive control (100 nM retinoic acid)

１）＊：５％水準で有意差あり
２）濃度はササ抽出物の濃度である
３）P.C.＝陽性対照（5 %FBS含有DMEM） (Test Example 3: Activation of fibroblasts)
The lotions of Example 2 and Comparative Example 2 were added to DMEM containing 1% bovine serum (FBS), and the fibroblasts were cultured for 48 hours. After culturing, cell activation was calculated using MTT reducing ability as an index, and the results are shown in Table 7.
It was shown that it was significant for the activation of fibroblasts when the concentration of the lotion extract of Example 2 was 0.01% or more.

1) *: Significantly different at 5% level 2) Concentration is the concentration of Sasa extract 3) PC = positive control (DMEM containing 5% FBS)

(Example 3, Comparative Example 3: Granule preparation for food and drink)
According to the formulation of Table 8, a granule preparation for food and drink containing 40% by weight of Sasa extract was produced by a conventional method.

(Test Example 4: Improvement of intestinal environment, improvement of liver function)
Divided into 2 groups of 10 adults each for 20 adults, the group of eating and drinking granule preparation of Example 3 and the group of eating and drinking granule of Comparative Example 3 were taken, and 2.5 g of the test sample was taken a day. Ingested once a month. The study period is from 1 week before taking to 5 weeks from the start of taking 1 month, and the amount of defecation, number of defecations, stool shape, color, smell, abdominal tension (fullness) and physical condition are improved by self-recording. In the case of the evaluation, the evaluation was made so that the score was high. Average values and standard deviations were calculated for 1 week before taking and 1 to 4 weeks after taking, and the degree of improvement was compared by a significant difference test. The results are shown in Table 9.

  At this time, a commercially available DEMECAL blood test kit was used to perform blood tests before and after taking the drug and compare the test values. Test items were 12 items of serum total protein, serum albumin, GOT, GPT, γ-GTP, total cholesterol, neutral fat, HDL cholesterol, urea nitrogen, creatinine, blood glucose and uric acid. Table 10 shows the results of 10 GOTs in the dietary granule preparation ingestion group of Example 3.

肝機能の改善

Improvement of intestinal environment

Improvement of liver function

(Example 4, Comparative Example 4: Eating and drinking tablet)
According to the formulation of Table 11, a tablet for food and drink containing 1% by weight of Sasa extract was produced in a conventional manner.

(Test Example 5: Allergy suppression)
Using 20 ovalbumin-induced food allergy model mice 10 weeks old, the control group and the test substance administration group were fed for 20 days. In the control group, a standard food (containing egg white) in which 5% by weight of the food and drink tablet of Comparative Example 4 was mixed, and in the test substance administration group, a standard food (containing egg white) in which 5% by weight of the food and drink tablet in Example 4 was mixed was used. I was fed. Note that the tablets were pulverized.
Allergy suppression was evaluated by the following method.
1. Skin eruption score (Erythema, wrinkle, dryness was scored in 4 stages, and the average value of the total score was compared. The worse the value, the higher the value.)
2. Serum IgE antibody amount (the amount of IgE antibody in serum was measured using an ELISA kit. It is known that allergy worsens with an increase in IgE antibody.)
The results are shown in Table 12 and FIG. The food and beverage tablet of Example 4 was found to be improved as compared to Comparative Example 4 with a decrease in the skin eruption score and the amount of serum IgE antibody over the entire period.

1. Skin rash score

(Example 5)
The Ehrlich tumor cell line was transplanted subcutaneously into the groin of 10 mice, and the day of tumor cell transplantation was defined as day 0. The Sasa extract of Production Example 1 was orally administered once a day from 1 week before tumor implantation to 2 weeks after implantation.

(Test Examples 6 and 7: Tumor suppressive action)
The tumor volume was measured on the 5th, 10th, and 14th days of transplantation, and the tumor weight was measured on the 14th day of transplantation, and the average values were compared. Tumor volume (mm ³ ) was calculated as major axis (mm) × minor axis (mm) 2 × 0.5.

(Test Example 8: NK cell activation)
The spleen was extracted from the mice that had been observed in Test Examples 6 and 7, and a cell suspension was prepared. This suspension was overlaid on the Ficoll-Conray solution and centrifuged to separate NK cells to obtain a NK cell suspension (referred to as E). NK cell activity after culturing a suspension of K562 cells (human chronic myeloid leukemia cells) labeled with 51Cr (200μCi) and preparing E: T = 50: 1. Activity was measured.

(Test Examples 9 to 11: Lymphocyte activation)
The spleen was removed from the abdomen of 5 normal mice to prepare a cell suspension. After adjusting the number of cells, each test solution, PBS (-), LPS (10 μg / ml) or Con A (10 μg / ml) and production are prepared in each well of the cytokine measurement plate (each n = 3 is used). 20 μl each of the Sasa extract of Example 1 was added and cultured for 72 hours, and IL-2, TNFα and IFNγ in the culture supernatant after centrifugation were measured by ELISA.

(Example 6)
(Test Example 12: antioxidant effect)
According to the method modified by T. Hatano et al. [Chem. Pharm. Bull. Vol. 37, (8) 2016-2021 (1989)], the Sasa extract of Production Example 1 and 1.0 g of each specimen shown in Table 16 Antioxidant activity of diphenyl, picryl, hydratyl (DPPH) radical 0.05 μmol, that is, the titer for eliminating 19.716 μg was measured as one unit.

(Example 7)
The following products were produced using the Sasa extract of the present invention. Although the formulation example is shown below, this invention is not necessarily limited to these.
1) Health food (tablets)
A composition containing the following additives was tableted to obtain a tablet having a good appearance.
% By weight 1. Dry powder 10 of Sasa extract of Production Example 2 3. Plant extract powder (Dokudami, carrot, perilla) 30 Royal jelly powder 5 4. Collagen powder 5 5. Lactose 25 6. Corn starch 20 7. Hydroxypropyl cellulose 4 8. Magnesium stearate 1
2) Health food (granule)
The food for health was manufactured by the following prescription.
% By weight 1. Dry powder of Sasa extract of Production Example 2 35. 3. Agaricus dry powder 15 Fructooligosaccharide 5 4. Guava leaf extract powder 10 5. Milk calcium 25 6. Vitamin E 10
3) Health drinks Health drinks were manufactured according to the following prescription.
% By weight Sasa extract of Production Example 1 4 Honey 15 3. Citric acid 0.1 4. dl-malic acid 0.1 5. Plant extract (chlorella, cinnamon) 20 6. D-sorbitol solution (70%) 10 7. Sodium benzoate 0.05 8. Perfume appropriate amount 9. Residue with 100 purified water
4) Fruit juice beverage A fruit juice beverage was produced according to the following formulation.
% By weight Glucose liquid sugar 33 2. Grapefruit juice 65 3. Sasa extract of Production Example 1 0.5 4. Perfume appropriate amount 5. Sour seasoning
5) Production Example of Lotion A lotion was produced according to the following formulation.
% By weight 2. Sorbit 2 2.1,3-butylene glycol 2 Polyethylene glycol 1000 1 4. 4. Polyoxyethylene oleyl ether (25E.O.) 2 Ethanol 10 6. Sasa extract of production example 1 1 7. Plant extract (Aloe, Hammamelis) 3 8. pH adjuster appropriate amount 9. Preservative appropriate amount 10. Residue with 100 purified water
6) Example of production of emulsion An emulsion was produced according to the following formulation.
% By weight Squalane 3 2. Petrolatum 1 3. Stearyl alcohol 0.3 4. Sorbitan monostearate 1.5 5. 6. Polyoxyethylene (20) sorbitan monooleate 3 6.1,3-butylene glycol 5 Sasa extract of Production Example 1 0.5 8. Water-soluble collagen 1 9. Preservative appropriate amount 10. Residue with 100 purified water
7) Production Example of Cream A cream was produced according to the following formulation.
% By weight Squalane 20 2. Beeswax 5 3. Refined jojoba oil 5 4. Glycerin monostearate 2 5. Sorbitan monostearate 2 6. 6. Polyoxyethylene (20) sorbitan monostearate 2 Glycerin 5 8. Sasa extract of Production Example 1 0.3 9. Bovine placenta extract 2 10． Preservative appropriate amount 11． Residue with 100 purified water
8) Production Example of Body Soap A body soap was produced according to the following formulation.
% By weight 1. Potassium laurate 15 Potassium myristate 5 3. Propylene glycol 5 4. Sasa extract of Production Example 1 3 5. pH adjuster appropriate amount 6. Preservative appropriate amount 7. Residue with purified water 100
9) Shampoo Shampoo was manufactured according to the following prescription.
% By weight 1. Lauryl sulfate triethanolamine 5 Polyoxyethylene lauryl ether sulfate Na 12 3.1,3-butylene glycol 4 4. 4. Lauric acid diethanolamide 2 Edetate disodium 0.1 6. Sasa extract of Production Example 1 2 7. Hydroxypropyl chitosan 0.4 8. Perfume, preservative appropriate amount 9. Residue with 100 purified water
10) Rinse A rinse was produced according to the following formulation.
% By weight 1. Stearyltrimethylammonium chloride 2 Cetostearyl alcohol 2 3. Polyoxyethylene lanolin ether 3 4. Propylene glycol 5 5. Sasa extract of Production Example 1 2 6. pH adjuster appropriate amount 7. Preservative appropriate amount 8. Residue with 100 purified water
11) Hair Nick The hair Nick was manufactured according to the following prescription.
% By weight Ethanol 50 Ethyl oleate 1 3. Polyoxyethylene (40) hydrogenated castor oil 2 4. 4. Plant extract (Emertium, assembly, Hamelis) 10 Sasa extract of Production Example 1 0.5 6. Residue with 100 purified water
12) Bath agent (A type)
A bath preparation was prepared according to the following formulation.
% By weight Sodium bicarbonate 562. Anhydrous sodium sulfate 30 3. Borax 2 4. Sasa extract of Production Example 1 10 5. Plant extract powder (camomile, toki) 2
13) Bath agent (B type)
A bath preparation was prepared according to the following formulation.
% By weight Refined jojoba oil 5 2. 2. Polyoxyethylene sorbitan monolaurate 20 Glycerin monostearate 5 4. Liquid paraffin 2 5. Lauric acid diethanolamide 3 6. 6. Dry powder of Sasa extract of Production Example 1 10 Plant extract (peony, aloe vera, chimpi) 10 8 Residue with 100 purified water

It is the figure which compared the amount of cytokine (IL-13) (Test Example 1). It is the figure which compared the amount of serum IgE antibodies (Test Example 5).

Claims (11)

  Bamboo leaves and / or persimmons are steamed at 160-190 ° C. for 30 seconds to 1 hour with dry steam whose dryness is improved by a pressure reducing valve and a gas-liquid separator, and then extracted with an aqueous solvent. The Sasa extract obtained by the manufacturing method of a Sasa extract.   A physiologically active composition comprising the Sasa extract according to claim 1.   The bioactive composition according to claim 2, comprising 0.001 to 50% by weight of Sasa extract.   The bioactive composition according to claim 2 or 3, which is an antiallergic agent.   The bioactive composition according to claim 2 or 3, which is a skin protective agent.   4. The bioactive composition according to claim 2, which is an intestinal environment improving agent.   The bioactive composition according to claim 2 or 3, which is a liver function improving agent.   The bioactive composition according to claim 2 or 3, which is an immunostimulator.   The bioactive composition according to claim 2 or 3, which is an antimutagenic agent.   A cosmetic comprising the physiologically active composition according to claim 2.   A food comprising the bioactive composition according to claim 2.

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