JP2001333731A - Food having immunostimulation activity - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a food having immunostimulation activity, not only having a prophylatic activity against disease by enhancing immunological competence and adjusting homeostasis balance thereof, but also capable of ameliorating anorexia, insomnia, stiffness in neck, cephalea or the like caused by stress, and exhibiting beauty effects (recovery of elasticity of the skin). SOLUTION: This food for immunostimulation contains one or more kinds selected from a degradation product of a milk protein, a degradation product of a silk protein and placenta extract. Preferably, the food for immunostimulation further contains one or more kinds selected from a nucleic acid, a β-1,3-glucan or a derivative thereof, a propolis extract, and an Agaricus extract.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to a method for enhancing immunity,
It also regulates homeostasis, creates a constitution that is less susceptible to illness, and provides immunostimulated foods that have the effect of improving anesthesia, insomnia, stiff shoulders, headache, or decreased skin elasticity (cosmetic effect) due to stress. .

[0002]

2. Description of the Related Art Western medicine has played a large role in treating infectious diseases. However, on the other hand, it is increasingly important to deal with such infectious diseases and adult diseases as preventive medicine in the aging society.

[0003] Although we are originally exposed to the invasion of viruses and microorganisms from the outside world and living inside in a state in which foreign self-materials (for example, cancer and self-dead cells) appear inside, It is possible to survive in such a situation because it has a mechanism (immunity) for removing such foreign substances and protecting itself.

[0004] In general, stress, infectious diseases (such as measles, influenza, and AIDS) and exposure to chemical substances are considered to be factors that lead to a decrease in immunity, and they decrease with age. Therefore, such a decrease or abnormality of the biological defense ability may cause various undesired diseases, and may cause unhealthy symptoms such as anorexia, insomnia, stiff shoulders, headache, and reduced skin elasticity.

[0005]

SUMMARY OF THE INVENTION The present invention has been made in view of such circumstances, and has been made to enhance the self-immune function, balance its homeostasis, create a constitution resistant to illness, and maintain health. It is another object of the present invention to provide an immunostimulating food useful for beauty.

[0006]

Means for Solving the Problems The immunostimulated food according to the present invention contains, as essential components, safe materials such as a milk protein digest, a silk protein digest, and a placenta extract. That is, these protein hydrolyzates and extracts function as active ingredients, and activate the reduced immunity to exert an effect of adjusting homeostasis.

[0007] The effect of the active ingredient can be further improved by using an auxiliary ingredient selected from nucleic acid, β-1,3 glucan or its derivative, propolis extract and agaric extract together with the active ingredient. be able to.

The milk protein hydrolyzate used in the present invention includes, for example, proteins such as casein, α-lactalbumin, β-lactoglobulin, albumin, immunoglobulin, lactoferrin, and lysozyme; And those having a molecular weight of about 500 to 10,000 are particularly preferred. As the protease, trypsin, chymotrypsin, papain, pepsin, rennin, pancreatin, elastase, carboxypeptidase, aminopeptidase, dipeptidase, alcalase and the like can be used.

[0009] As for the silk protein hydrolyzate, a protein called silk fibroin prepared from silk can be used as a raw material. As the production method, a general method, that is, a decomposition method using an acid or an alkali, or a hydrolysis method using a protease as described above can be used. Molecular weight
Peptides in the range of 300 to 10,000, centered around 500 to 5,000, can be suitably used, but need not necessarily be fractionated and purified.

[0010] The placenta extract is, for example, crushed placenta such as cattle and the like, and oil-soluble components are removed using a solvent such as butanol, hexane and ether, and then water or water containing salts such as ammonium sulfate is added. A water-soluble extract or a placenta-decomposed product obtained by hydrolyzing a crushed placenta using the above-mentioned acid, alkali, proteinase or the like can be used.

As the nucleic acid to be used as an auxiliary component, for example, an oligonucleoprotein mainly composed of an oligonucleotide and an oligopeptide obtained by treating a nuclear protein with the above-mentioned protease is used.

[0013] β-1,3 glucan is a polysaccharide of D-glucose mainly composed of β-1,3 bonds, and those derived from yeast cell walls or fungi can be used. β-
1,3 glucan derivatives include hydrolyzed β-1,
3-glucan oligomer, hydroxyalkyl (C2-4)
Β-1,3 glucan, carboxymethylated β-1,3 glucan, sulfated β-1,3 glucan, phosphorylated β-1,3
Glucan or the like can be used.

[0013] The propolis extract is composed of bees mainly composed of a mixture of sap collected from tree buds of eucalyptus, pine and the like, sap collected from the bark of the tree and enzymes contained in beeswax and saliva of bees. , Water, ethanol or an extract obtained from a mixture thereof.

The agaric extract is Agaricus (Agaric)
us blazei Murill) is a β-protein obtained from a mushroom belonging to the family Agaricaceae (Agaricus.L: Fr.).
An extract mainly containing neutral and acidic polysaccharides and nucleic acid components mainly composed of β-1,6 glucan having 1,3 sugar chain branches is used.

The food for immunostimulation according to the present invention is, for example,
Processed foods, confectioneries, beverages, internal preparations (including preparations used for animals) in general, in terms of morphology, ampules,
Capsules, pills, tablets, powders, granules, solids, liquids, gels, bubbles or the like can be optional. Specifically, general products such as beauty / healthy beverages or foods (vitamin supplementation, nutritional tonicity, recovery from fatigue, skin roughness improvement, body structure improvement, hair growth, hair growth / hair restoration), Western confectionery, Japanese confectionery, gum, candy, caramel, etc. General soft drinks such as confectionery, fruit juices, processed seafood products such as kamaboko and chikuwa, livestock products such as sausage and ham, noodles such as raw noodles, boiled noodles, buckwheat, sauces, soy sauce, sauce, sugar, honey, Seasoning such as powdered candy, starch syrup, spices such as curry powder, mustard powder, pepper powder, jam, marmalade, chocolate spread,
Dairy products such as cheese, butter, and yogurt are included.

In the present invention, the food for utilizing the immunity is adjusted so that 50 mg to 20 g / day of one or more active ingredients selected from milk protein decomposed products, silk protein decomposed products, and placental extracts can be taken. Is desirable.

One or more auxiliary components selected from nucleic acids, β-1,3 glucan or derivatives thereof, propolis extract and Agaricus extract may be used in an amount of 1/10 to 1/3 times the amount of the active ingredient. Good to use.

[0018] In addition to the above-mentioned essential components, the components and additives exemplified below can be optionally selected and used in the food for utilizing the immunity in the present invention, if necessary.

(1) Various fats and oils Avocado oil, almond oil, fennel oil, perilla oil,
Olive oil, orange oil, orange luffer oil, sesame oil, cocoa butter, chamomile oil, carrot oil, cucumber oil, tallow fatty acid, kukui nut oil, safflower oil, shea butter, liquid shea butter, soybean oil, camellia oil, corn oil, rapeseed Oil, persic oil, castor oil, cottonseed oil, peanut oil, turtle oil, mink oil, egg yolk oil, palm oil, palm kernel oil, mocro, coconut oil, tallow, lard, or hydrogenated products of these oils (hardened oil, etc.) )etc.

(2) waxes Beeswax, carnauba wax, whale wax, lanolin, liquid lanolin, reduced lanolin, hard lanolin, candelilla wax,
Montan wax, shellac wax, rice wax, squalene, squalane, pristane, etc.

(3) Fatty acids Natural fatty acids such as lauric acid, myristic acid, palmitic acid, stearic acid, behenic acid, oleic acid, 12-hydroxystearic acid, undecylenic acid, tall oil, lanolin fatty acid, isononanoic acid, caproic acid And synthetic fatty acids such as 2-ethylbutanoic acid, isopentanoic acid, 2-methylpentanoic acid, 2-ethylhexanoic acid and isopentanoic acid.

(4) Alcohols Natural alcohols such as ethanol, lauryl alcohol, cetanol, stearyl alcohol, oleyl alcohol, cholesterol and phytosterol, and synthetic alcohols such as 2-hexyldecanol, isostearyl alcohol and 2-octyldodecanol.

(5) Polyhydric alcohols Ethylene oxide, ethylene glycol, diethylene glycol, triethylene glycol, ethylene glycol monoethyl ether, ethylene glycol monobutyl ether, diethylene glycol monomethyl ether, diethylene glycol monoethyl ether, polyethylene glycol, propylene oxide, propylene glycol , Polypropylene glycol, 1,3-butylene glycol, glycerin, pentaerythritol, sorbitol, mannitol and the like.

(6) Esters Isopropyl myristate, isopropyl palmitate, butyl stearate, hexyl laurate, myristyl myristate, oleyl oleate, decyl oleate, octyl dodecyl myristate, hexyl decyl dimethyloctanoate, cetyl lactate, Myristyl lactate, diethyl phthalate, dibutyl phthalate, lanolin acetate, ethylene glycol monostearate, propylene glycol monostearate, propylene glycol dioleate and the like.

(7) Gum, sugar or water-soluble polymer compound Arabic gum, benzoin gum, dammar gum, guaiac oil, Irish moss, karaya gum, tragacanth gum,
Carob gum, quinseed, agar, casein, lactose,
Fructose, sucrose or an ester thereof, trehalose or a derivative thereof, dextrin, gelatin, pectin, starch, carrageenan, carboxymethyl chitin or chitosan, hydroxyalkyl (C2-C4) to which an alkylene (C2-C4) oxide such as ethylene oxide is added. )
Chitin or chitosan, low molecular weight chitin or chitosan, chitosan salt (organic acid, inorganic acid), sulfated chitin or chitosan, phosphorylated chitin or chitosan, alginic acid or salt thereof, hyaluronic acid or salt thereof, chondroitin sulfate or salt thereof, heparin , Ethyl cellulose, methyl cellulose, carboxymethyl cellulose, carboxyethyl cellulose, sodium carboxyethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, nitrocellulose, crystalline cellulose, polyvinyl alcohol, polyvinyl methyl ether, polyvinyl pyrrolidone, polyvinyl methacrylate, polyacrylate, polyethylene oxide and Polyalkylene oxide such as polypropylene oxide or its crosslinked polymer, carboxyvinyl Rimmer, polyethylene imine.

(8) Various vitamins Vitamin A group: retinol, retinal (vitamin A
1), dehydroretinal (vitamin A2), carotene, lycopene (provitamin A), vitamin B group: thiamine hydrochloride, thiamine sulfate (vitamin B1), riboflavin (vitamin B2), pyridoxine (vitamin B)
6), cyanocobalamin (vitamin B12), folic acids,
Nicotinic acids, pantothenic acids, biotins, choline,
Inositols, vitamin C group: vitamin C acid or a derivative thereof, vitamin D group: ergocalciferol (vitamin D2), cholecalciferol (vitamin D3),
Dihydrotaxelol, vitamin E group: vitamin E or its derivatives, ubiquinones, vitamin K group: phytonadione (vitamin K1), menaquinone (vitamin K
2), menadione (vitamin K3), menadiol (vitamin K4), other essential fatty acids (vitamin F), carnitine, ferulic acid, γ-oryzanol, orotic acid, vitamin Ps (rutin, eriocitrin, hesperidin), vitamin U etc.

(9) Various amino acids valine, leucine, isoleucine, threonine, methionine, phenylalanine, tryptophan, lysine, glycine, alanine, asparagine, glutamine, serine, cysteine, cystine, tyrosine, proline, hydroxyproline, aspartic acid, glutamic acid , Hydroxylysine, arginine, ornithine, histidine, etc., and their sulfates, phosphates, nitrates, citrates,
Or amino acid derivatives such as pyrrolidone carboxylic acid.

(10) Various additives derived from plant or animal raw materials These are processed in a conventional manner according to the type and form of the product to be added (for example, pulverization, milling, washing, hydrolysis, etc.). , Fermentation, purification, squeezing, extraction, fractionation, filtration, drying,
Powdering, granulation, dissolution, sterilization, pH adjustment, deodorization, decolorization, etc. may be arbitrarily selected and combined), and selected from various materials.

The solvent used for the extraction may be selected in consideration of the purpose and type of the product to be provided, or the processing to be performed later. However, usually, water or a water-soluble organic solvent (for example, ethanol) is used. ,Propylene glycol,
It is preferable to use one or a mixture of two or more selected from 1,3-butylene glycol). However, when the content of the organic solvent is not preferable depending on the application,
Only water may be used, or ethanol which is easily removed after extraction may be used, and may be used alone or in any mixed solution with water, or may be extracted and extracted.

[0030] By using these together, healthy and cosmetic effects such as nutritional supplementation, recovery from fatigue, tonicity, cell activation (prevention of cellular aging), blood pressure improvement, blood flow promotion, body temperature regulation, anti-inflammatory, whitening, hair growth and the like can be obtained. Initially, it is possible to expect effects such as improvement of taste, color tone and aroma, stabilization, thickening, preservation, etc., and by combining these, aim to increase the intended effect, or multifunctional effect It is also possible to make a product that expects.

Specific plants (crude drugs) as raw materials include:
For example, almonds (Hentomo), eye (Aiba), Blue Kazura
(Seifu Fuji), Aoki (Aoki), Aokiri or Kenasia Ogiri
(Otogi), Aotsujirafuji (Kibo), Acacia, Akasho (Akasho), Akasuri `` Fruit '', Red clover, Red grape, Akaminoki (Logwood), Red acacia (Red bud), Akane (Akane grass root), Red acacia or Jio ( Ground yellow), Agi (Awei), Aki Larch, Akinire (Latinaceae),
Aguay Guacu, Aguahe (Omitengu palm),
Akebi (Kidori), Asa (John Asako), Malva Asagao or Asagao (Toshibushi), Hydrangea (hydrangea), Ashitaba (Ashitaba),
Azuki (red azuki), acerola, asenyaku (Asenyaku), acira (shokuyoukanna), anise, abiu, abiurana, rape, avemaki `` fruit '', avocado, ama, amacha (sweet tea), amachaworu, amadocoro (tamatake), Amana (Kikomiko), Amaranthus (Amaranthus), Amaranthus hippochondriacus, Haribiyu, Amaranthus hippochondriax, Amigasa lily or Bimo (shellfish),
Aristolomeria (Yurizisen), Arcana (Alcanna), Algarrobo (Cabbage), Altea, Arnica, Alpinia or Katsumadai (Souduq), Aloe (Length), Aloe vera, Angelica, Apricot or Hong apricot (Anjin), Ansokkou (Azusa), Yellow sapote , Ikakozorina (ground bile head), Epimedium or Epimedium
(Inyokaku), Igusa (Wicked grass), Knotweed (Tiger root),
Strawberry (first place), strawberry, fig (Fig.
Leaf)), Ichihatsu (first time), Ichibi (Fuyu aoi), Ichiyakuso (one medicinal herb), Ginkgo (Ginkgo `` seed, leaf ''), Itohimemegi (Enshi), Locust bean, Inu-zuna (Telelexi), Inubeyu (Sorainubiyu), Rice `` seed, seed coat '', Inondo `` seed '', Ibuki-kusou-so, nettle, ylang-ylang, iwa-tobacco (iwatsuchi-chi), iwahiba or iwamatsu (makikashiwa), oolong tea, wikyo (fenka), wheat (tibusanoki), ukiyagara (miyoko) ), Uguisukagura "fruit",
Himeuikyo, turmeric (Utsukin), duckweed (buoying), Usubasaishin or Keirinsaishin or Shousaisaishin (spicy), Usvenia oi, Usvenitai oi, Utsubogusa (summer dry grass), Udo or Shishiudo (Qangji, Independent, Tang Independent) ), Unyadegato (Unkaria, Cat's claw), Uba tea, Ume (Ume `` seed, pulp ''), Urajirogashi, Uwaurushi (Kusakokomomo), Unshu mandarin (Chen skin), Estragon, Ezocogi (Ezogoka), Echinashi (Hosobamurasakikirengiku) , Enishida, Enokitake (Enokitake), Ebisugusa or Cassia Tora (Keiko), Elcampre, Elderberry `` fruit '', Elemi, Elingy or Prelotus elinge, Endu (Gossip, Sophoraceae rice), Ogi or Kibananagi (yellow) Hoki), Ousei (Naruko lily, Kagi Kurumabanarukoyuri), OuHisasa Yuki or Sasanoyuki, Coptis (Coptis), Oogatahouken, giant Karasuuri (Karokon), Ooguruma (civil engineering incense), giant Tsudzurafuji
(Self defense), psyllium (vegetative child, vegetative grass), toucan hazel (haruko), toucan cactus, pygmy plant or okera
(White jujube), giant leaf sedge (banaba), giant leaf beetle "fruit", giant squirrel (grain sperm), giant caterpillar, giant hawthorn or hawthorn (yamashiko), barley
(Barley), oka, okazeri (snake bed), okra `` fruit '', ogulma (turnover), panax ginseng or ginseng (carrot), hypericum or hypericum perforatum or perforated periwinkle (brother cut grass), dragonfly (continuous cut), onamimi (blue) Ears), Onigurimi, Onidokoro or Tokoro or Nagadokoro (Hikai), Oninoyagara (Tianma), Oniyuri or Sasayuri or Hakatayuri (Yuri), Ononis, Halibut
(Hihayu), Ominaeshi (defeated), Oyuco, Dutch mustard (Watercress), Dutch jelly, Dutch honeywort, olive "fruit, seed, leaf", oregano, orange "fruit, pericarp", silkworm (ripened yellow) , Cocoa "fruit, peel,
Seeds '', oysters (persimmons `` leaves ''), kagi kazura (crop hooks), oysters or kantosou (rensensou), gaku hydrangea, cassia, casino ki (crocodile fruit `` fruits ''), gajutsu (shadotsutsu), Kashiwa
(Sakura tree, Sakhalin), Kascarilla, Kascara sagrada, Gypsophila, Kasensou, Kanixa (Kinosato), Kaniwa, Carnation, Kanokoso (Yoshikone), Birch or Shidare kamba (White birch), Gerbera, Pumpkin, Capoknoki `` Seeds '', Kahoku Sansho (shopper), Gama (bamboo), Chamomile or Roman chamomile, Camellia japonica (weed), Camu cam (camouflage), color, Karaku Sakeman, Ragwort or Chinese Ragwort (Our melon), Ragwishak (midsummer), Ragwort, Guarana `` Seeds '', Karaho (thick), Karaya, Galu, Karin (Gourd melon), Garcinia, Kawamidori, Kawara Psycho (Seiryuna, Hakubaku), Kawazusha, Kawatake, Kawaranadeshiko (Ishitake) or Ezokawaranadeshiko ), Japanese carrot (Aoko), Japanese sagebrush (Inchinko), Kansui (Kansu), Licorice Kantarasa, Candelilla, Kanto, Canna, Raspberry (Ezo strawberry, Dutch strawberry, Shrimp strawberry, Nawashiroi strawberry, Maple strawberry, European strawberry), Kiwi `` Fruits, leaves '', Kiwiasuuri (Uroro root), Kikyo (Kikyo, Kikyo root), Kiku (Kikyo) Chrysanthemum flower, oak daisies, daffodils, kikutani daisies, catalpa (Azumizumi), daisies (sheep-of-ears), pheasants (kokomi), kizuta, kidachi aloe, lucky mint, quina, linden (cincona, red linden), quinoa (quinoa), cinnamon (yellow) ), Gymnema Sylvester,
Kimimaku, cabbage, cabbage `` immature fruit '', cucumber, gyoryu (Nishikawayanagi, Ichiyanagi), Kiraja saponaria, Kiraya, Kyransou (gold acne grass), Kumquat `` fruit '', Ginkai, Kimbusen, Kinma, Kinmizukiki
(Sengaku grass), guapeba vermelilla, guava `` fruit '', guayule, quelux infectria (gallicate), kukui nuts, wolfberry (glygi, gugiko, gugi leaf, skeletal bark), kusagi-kazura (tenmon winter), kuzu (kuzukane) ), Camphor tree, gooseberry `` fruit '', ginseng, yellowtail, gardenia, gardenia, blackberry,
Kupuas, Kumazasa, Kumatsuzura (horse whipweed), Clara (bitter butter), cranberry "fruit", chestnut "seed, fruit, astringent skin", curculigo latifolia "fruit", grapefruit "fruit / leaf", buckthorn, black stalk
(Rescue rescue), Kurobana Hikikoshi or Hikikoshi (extended life grass), cloves (cloves, cloves), gumbinazuna (sekimei, sekimeishi), kaigai (infested, infested ears), celosia
(Cow flower, rooster), geccavidin, bay (laurel), kenashi sardine rose (Tsubukuryo, Yamakiko), gentiana, gennoshoko (Older's grass), kenponashi (Kigushi),
Koukisekoku, Kishumitsukan (Koji, Tachibana,
Olivenimikan, Fukuromikan, Sagamikouji, Ponkan, Santara (Tachibana skin)), Koushinbara (Monthly flower), Kosuui mint, Kozo `` fruit '', Kocha (tea), Kohone
(River bone), kouhon (straw and kara straw), kouriang, kourikyo (takara kang), cilantro `` fruit '', koren
(Hou Lian), Scutellaria (Yellow Gon), Cowberry (Koshibachi), Coco Palm `` Fruit '', Goshuyu (Goshuyu), Goshostrawberry (Uboniko), Pepper (Hot Pepper), Cosmos, Copaiba Balsam,
Coffee `` seed, leaf '', kobushi or magnolia (hot spring), burdock (burdock, burdock), kobotdur, sesame (sesame), sesame hahagusa (gensen), goji (gomiko), Sanekazura or binankazura or Matsubusa, wheat (wheat) , Rice or rice bran “red bran, white bran”, rice oil, cola acuminata “seed”, cola vera “seed”, colony llah, fenugreek “fruit”,
Colombo, Konzulango, Kombu, Konjac, Comfrey (Shinbari grass), Sisal (Sisal Asa), Sakisima button or Sinata button or Sinensensou (Eisensen), Sakura (Oshimashima cherry, Yamazakura, Oyamazakura, Edhigashi, Mamezakura, Miyayamazakura, Somei Yoshino, Takanezakura, Kasumizakura, Kohigan, Satozakura, Kanzakura `` leaves, flowers, fruits, bark (cherry bark) ''),
Cherries, pomegranate, sasa, sasanqua, sagemodaka
(Sawashatsu), sweet potato, sugar cane, sugar beet, sapling jujube (acid jujube), saffron (safflower, west safflower), sapodilla, sapotea marillo, pomelo `` fruit '', savonso, salmon berry `` fruit '', sarasinachoma (masuma), Salvia (sage), Sawagyoukyo (Yamakosai), Sawagumi (Yamawomo), Sankaku cactus, Sangre degrad (Croton), Sansouyousou, Sanshin carrot
(37 ginseng), sanshuyu (sanshuyu), sansho (sansho),
Sandscon (Yamazone), Shea (Karite), Cyanoki `` fruit '', Shiitake (Shiitake), Shion (Shion), Dikitaris, Shikunshi (Kimiko), Perilla or Aodiso or Chirimenjiso or Kamenjiso (Shiso leaf, Shiso), Rosewood, linden,
Chinese pine, Chinese forsythia (forsythia), Shimeji (Hinshimeji, Shakashimeji, Hatakeshimeji, Oshiiroshimeji,
Bunashimeji, honjimeshi, shirotamogitake), shimokuren (hot spring), shimotsukeisou, potato, peonies (shakuyaku), shajin (shasan), jasmine (matsurika), janohige (wheat gate winter), shukukonemisou, shukushamitsu (sunin, Shredded sand), jujudama, palm `` fruit '', ginger (ginger), giant hydrangea (Tsuneyama), shobu (iris, iris root), shojiku `` fruit '', shirakashi `` seed '',
White butterfly (seed), white clover (clover), white adzuki (chicken bone grass), white iris (small iris), white natto (flower), white nanten (minamitenmi),
Cinchona Sacrifura, Jinjuge (Mizuka, Mizuka,
Cinnamon), cinnamon, cinnamomum cassia (cinnamon bark), watermelon (watermelon), honeysuckle (gold and silver flowers, shinto winter), sorrel (acid model), swimberry "fruit", star apple, stevia, strawberry "fruit", tin shiko ,
Horsetail (Thorn), Purslane (Horse Tongue, Horse Tongue), Plum (Fruit), Pinus sylvestris (Cones), Larix sylvestris, European Lentils, European Walnut, European Hawthorn, European Dandelion, European Chestnut (Marronnier), Pear "fruit", snow pear,
Sambucus elder (Elder), Atlantic shrew (Juniper, Juniper), Achillea millefolium (Mirfoil), Atlantic rose, Aurora magnolia, Atlantic mistletoe, Western mackerel or Western mackerel, Western horseradish, Sekisho (stone irises) Cedron (Bowshukuboku), Zeniaoi, Hirohasenega, Senega, Seri, Celery, Senkyu (River Kyu), Senshinren (Shingenren), Sendan, Sennin Cactus, Senna `` Fruits, Leaves '', Senninsou (Otatate), Senbri ), Souka (grass), elephant, rosacea, buckwheat `` seed seed '', soybean potato, rhubarb (daisou), radish, soybean, daidai (orange skin, kikomi), takasaburo (dry lotus grass), takatodai (daigo), takawarabi (Inu), Dark Sweet Cherry "Fruit", Tachioi, Tachiji High-rise (time,
(Marika), Tachidokoro (Hikai), Tachiyanagi, Tamarind `` seed '', onion, tamshiba (spicy), Taranoki `` fruit, leaf, root bark '', dahlia (tenjikubotan), tarwi,
Tanshiniwa, Tanjin (Dan ginseng), Dandelion (Gankoei) or Shirobana dandelion or Moko dandelion, Danmar, Cherry `` fruit '', Tigaya `` fruit, root, bud '', Chixet carrot (Bamboo ginseng), Chicory, Chancapiedra (Kidachiko Mikansou) , Datura (Rhuanghuang), Datura elm (Wuyi), Datura mugwort (Aiba), Choreimaitake (Irei), Chorogi, Tsukimisou, Tsukuritake (mushroom), Camellia, Camellia, Tsumekusa (lacquer primrose), Camellia Ducklings), Tsuruazuki (red azuki bean), Tsurukudami
(Hanshu crow), Tsuruna (Ban apricot), Crucian carrot (Four leaf ginseng), Tsuwabuki, Deiko, Teuchigurumi, Duberry `` Fruit '', Tulip, Chocolate stay, Tengusa, Tencha (Tencha), Tendaiyaku (Wood medicine), Touga (Winter ginger) ), Squash, capsicum (pepper), squid (return), calendula (marigold), tonabena (river cut), corn or corn hair (southern hair), horned beetle (female child), torindo or kosen Gentian,
Tokusa (wood pirates), dokudami (jujutsu), konkon (sore root), sashimi or mamedaoshi or scutellaria, eucommia (mushroom `` bark, leaves, roots ''), ash (qin bark), tomato, tragacanth, triacanthos `` seed '', Turkey Kikyo, Tormentilla, Dronoki, Troloa oy, Niseria berry "fruit", Chinese yam or Yamanoimo (Yamayaku),
(Butcher Bloom), Naginata Kouju, Nazuna, Rape, Natsume Bean or Hatehaki (Sword Bean), Natsumikan, Jujube (Daijutsu), Naniwaibara (Kanakurako), Navena (intermittent), Nameko, Narco Lily (Yoshimi), Peanut (Peanut) ), Nanten (Minami Tenmi), Namban Karamushi (ramie), Niois violet,
Nigaki (bitter tree), bitter mugwort (bitter mite), nutmeg, kei or nikkei or ceylon nikkei or yabnikkei (cinnamon bark)
Or Keishi (Keishi), Chinese chive (Nirako), elderberry (bone-bearing tree `` fruit, flower, stem, leaf ''), garlic (garlic), nulde (fivefold child), leek, nemoki or neb or nebi or nemlinoki or zigokubana ( Healing), thistle (daiji), noibara (real fruit), nozuka quail (ryohana), nogateto, yarrow, nodoke (maehu), nobara, peach, pineapple `` fruit '', hibiscus (bussouge, hurinbussouge, roselle), hayindow, Hauchiwa, Pau Doce, Hakamauraboshi (bone crushing), Hakusen (ringworm skin), Huckleberry `` Fruit '', Hakobe (proliferation), Hashibami (Haruko),
Hasiridokoro (rotor root), basil, lotus (lotus, lotus, lotus root), parsley (Netherland jelly), naked mussels, batata, bees, or Madake (bamboo-boiled), patchouli, mint (light-weight, light-weight leaves), adlay (yokunin) , Hanasuge (Chimo), Banana, Hanamint, Hanabishi (Siturishi, Shisitsuri), Vanillabins, Papaya, Scotch (Rice koji), Pahalobobo, Hub `` Whole grass, stem, leaf '', Paprika, Hamago or Hitsuba Hamago Child), Hamasuge (Kazuke), Hamabishi
(Ritsuko Riko), Hamanas (maikai flower), Hamabofu (beach storm), Hamamelis, palm, rose (rose), valata,
Balata rubber tree, agaric mushroom (aratake mushroom) Bamboo tree, breadfruit, giant gourd (radish), lycopodium carrot (party), pecan nut, giganbana (garland, vinegar), higuru madaria, haishi (hishomi), pistachio, beet, hitoshiba (stone reed), Aedes aegypti, daisies (daisy), hinata nokozuchi (cow knees), hinoki, hiba, castor, sunflower,
Bell pepper, Himeuzu (Tamaoi), Himegama (Kaba), Himematsutake (Kawariharatake, Hiromatsutake), Pimenta `` Fruit '', Juniper, Biancucuum, Hyacinth `` Fruit '', Belowudo Oi, Hiroha Okinagusa (Hakuto Okina), Loquat `` Fruit,
Leaves, stems ”, betel nut (large clothing skin, betel nut), puer tea (
? Tea), spruce buds (southern rattan), butterflies, coltsfoot dandelions (winter flowers, winter leaves), fujibakama (orchid grass), wisteria beans (flat beans),
Fujimodoki (choujizakura, shikahana), grapes `` fruit, peel, seeds, leaves '', beech, fuyumushinatsukutake (Cordyceps sinensis), Brazilian liquorice, Brazilian carrots, black currant `` fruits '', blackberries, blanin,
Plum `` fruit '', flucellaria, blueberry (Acacia catechu), prune, floral blanka, blond psyllium, bundou (green bean), hazelnut, loofah, safflower (safflower), henneken, belladonna, berry `` fruit '', Percea, Perbianberg, Pereskia
Grandifolia, Bensui beetle or duckweed (Keiten), Boysenberry `` fruit '', Broomweed or Elderweed or Nendow or Hawakigi kokia (dermis), Baldwink (Hosen, acute child, osteophyte), Potteria sapota, potteria Lucuma, spinach, boufu (windbreak), spinach, physalis (Torone), honoki (Japanese magnolia, park), bokeh
(Gourd melon), Hosoba aburagiku (bitter gourd), Hosoba oguruma,
Hosovanaokera (Aojutsu), Hosobanokirinsou (Kageten 37), Hosobabarengiku, Bodaiju (Bodaiju), Button
(Peony `` flowers, leaves, stems, bark ''), buttonpies (peony bark), hops, jojoba, borage (ruriichisha), bordeaux, white berries `` fruits '', hon-onik (meat porcelain, daigei), hon-sekko (iron Peeling stones, sparrow mountain stones), myzuruten nansha (Tennan star), maitake (maitake), ephedra (mao), maca, macadamia nuts, margaret (mokushungiku), macri (seaman grass), maguwa (mulberry white bark) `` Bark, leaves ''), Magnolia splengeri, Masaranpouba, Masaranzuba do Ceara, Masheira de Boi, Masahua (Tamanouzenhallen, Cucumber kinrenka), Matatabi (Kiten Tate), Matsukasa, pine `` leaves, bark, Root, Matta Oryo, Matsuhodo (Bukuryo), Matico (Cordoncillo), Mayorum (Hanamint), Malvanojazine (Bittersweet), Mulberry "Fruit", Quince, Murray (Below mou watermelon), mango, mangosteen, mansagna (andesukamitsure), mansugurumi, mandarin `` fruit '',
Mannentake (Lingzhi), Pheasant (Kikomi `` fruits ''), Mishimasaiko (Saiko), Mizuobako or Mizuasagao (Ryugoshi),
Mizokukushi (Hanbe Lotus), Misohagi (Chisatsuna), Mizunyanagi or Shiroyanagi (Henryoku), Mitsugashiwa, Mitsuba, Midorisapote, Midorihakka, Mimosa, Myoga, Miracle Fruit `` Fruit '', Myrrh, Milobalan, Wheatwaragiku, Mukuge (Mokuge) , Mukunoki, Mukuroji (prolonged skin), Munya, Purple (purple root), Purple or purple
(Purple pearl), purple corn, purple purple (Kunming chicken blood wisteria), megakanza opuntica, mehajiki (mother grass), mebougi, melaleuca, melissa, melilot,
Melon `` fruit '', sagebrush, moso bamboo, moku giraffe, moje (koshoboku), mock berry (wood incense), maple blossom, peach (peach `` leaves, seeds, flowers, fruits ''), sprouts, morello cherry `` fruits '', Moroheiya (Burlap), yakawamurasaki potato, yakuchi (masuti), cornflower mackerel (cornflower), cornflower mint, yacon, yashabushi (arrow wheel) or himeyashabushi or giant yashabushi `` fruit,
`` Peel, ears '', willows, yatsude (octagonal gilded), mistletoe (parasitic willow), willows (kawayanagi, tachiyanagi, weeping willow, red willow, pussy willow, dogwood willow,
(Sweet willow, koreanagi, unryuyanagi, yamayamayanagi, palm, yamayanagi, giant psyllium, tyrichkinuyanagi, foxwillow, bonsai), willow `` leaf,
Stalk '', scabbard, savannah koji (purple beef), savory tobacco
(Tsurusu, Tenmei), pokeweed (commercial land), yamanoki (yamaharu), yamamomo (yangbai skin), sagebrush, eucalyptus, yukinoshita (tiger ear grass), yucca or flevifolia, yuzu `` fruit '', lily, yorigusa , Mugwort (Aiba), reigan (Rainuma), lime "fruit", rye, lakanka "fruit",
Raspberry `` leaves, fruits '', latania (Clameria), Rakkyo or shallots (Shiroi), lavender, longan (longan meat), agave (Aono agave,
Fukurin agave), Ryokucha (green tea), apple `` fruit, seeds, leaves, roots '', gentian, rubus or suavisimus (sweeten), litchi (lichi, lichi nucleus), lettuce (chisha), red currant `` fruit '', Red pitaya, lemon `` fruit '', lemongrass, forsythia or Chinese forsythia (forsythia), astragalus, wax bye, wax palm,
Lokon (reeds and radishes), Loganberry "fruit", rosemary (mannan wax), rose hips (nobara), wasabi, cottonweed cypress (mitsumenbana), waremoko (jimyu) and the like.

In addition, other seawaters, for example, deep water, seawater salt, dried seawater, inorganic salts obtained from the Dead Sea or the Atlantic or Pacific Ocean (sodium chloride, magnesium chloride, potassium chloride, etc.), sea mud or mud (Fangos), for example, sea mud or mud of various places such as Italian Fango, German Fango, Eifel Fango, Freiburg Fango, etc. (Ingredients: silicon dioxide, titanium dioxide, aluminum oxide,
Iron oxide, manganese oxide, sodium oxide, potassium oxide, magnesium oxide, calcium oxide, strontium oxide, sodium, potassium, magnesium, calcium, chromium, iron, copper, nickel, zinc, lead, manganese, arsenic, water), saintokuishi etc.

Examples of the seaweeds include seaweeds [green algae: Chlorella vulgaris, Chlorella pyrenoidosa, Chlorella ellipsoidia, Aonori (Usubaonori, Susiaonori, Hiraaonori, Bowaoonori, Hosoedaoonori), Anaaousa (Aousa) and the seaweed [Aosa] Kinds: Kombu (Macambu, Rishikonbu, Hosomekombu, Mitsuikonbu), Wakame, Hirome, Ao-Wakame, Giant kelp (Macrostistis pilifera, Macrocystis integrifolia, Neocitys luetokeana), Hijiki, Hibamata, Umiuchiwa, Usubaumiuchiwa Klebanoumi prickly pear, Akabumi prickly pear, Konau prickly pear, Okina prickly pear, Usuki prickly pear, Echinoki prickly pear], seaweed [red algae: Hijirimen, maxa (Tengusa), hirakusa, oniku , Obakusa, Kataobakusa,
Yatabegusa, Yuikiri, Shimatengusa, Tosakanori, Togekirinsai, Akumasakirinsai, Kirinsai, Junyashinkirinsai, Tsunomata, Obatsunomata, Tochaka (Yahatsutsunomata), Ezotsunomata, Togetsunomata, Hirakotonota, Kotojita
Jareus Banori, Kagius Banori, Siusius Banori, Hyus Banori, Akamomininori] and the like.

Other algae, for example, green algae (Chlamydomonas: Chlamydomonas, Akayukimo, Dunaliella: Dunaliella, Chlorococcus: Chlorococcus, Kwanomimo: Kwanomimo, Volvox: Ohikawawari, Volvox, Palmera, Yotsumemo, Spirogyra: Hizaori, Spirogyra, Tulsimidro, Hibimidro: Hibimidoro, Aosa: Anaoasa, Amiosa, Nagaosa, Kawanori: Kawanori,
Genus Fricciella, genus genus: medusa, asiaticum gutta, kawashiogusa, marimo, baronia: tamagobaronia, tamabaronia, tamagata: tamagatamo, iwata: husaiwata, surichogita, heraiwata, kurokita, kurokita, kurokita Sakibuchimil, Nagamir, Hiramil, and Casanori:
Casanori, Jusmo: Fusuzumo, Tamazumi,
Species of the genus Mizudzumo, Genus Genus, Genus Colecaetus, Genus genus Tsutsumo, Artemisia spp .: Artemisia spp.

Blue-green algae (Genus Lynchium: L., L., L.), Genus: L., mushrooms,
(Hassay, genus genus, genus racenmo (spirulina): spirulina, genus trichodesmium (aiakacio), etc.).

Brown algae (Piraella spp .: Piraella spp., Spirogyra spp.
Genus Isogawara: Isogawara, Kuroshira Genus: Gunsenkurogashira, Kashirazaki Genus: Kashirazaki, Muchimo Genus: Muchimo, Hiramutimo, Kebelligsa, Amidixa Genus: Amidigsa, Sakibiroamiji, Sanadagusa: Sanadagusa, Fukurinamiji, Common Genus Artemisia spp.
Genus genus: genus pine, genus genus: genus genus: genus genus, genus okinawa, genus okinawazuku, genus phlebosum, genus phlebosum, genus phlebosum: genus futomozuku, genus genus moss: genus genus: genus kuromo, genus moss, genus genus Ishige, Irolo, Icmegasa: Ichimegasa, Keyari: Keyari, Umibos: Umibos, Urushigusa: Ursigusa,
Betula serrata, Tobacco moss, Laminaria spp. Genus Kagomenori: Kagomenori, Muraridori: Murachidori, Shakuganusa: Shakuganusa,
Genus Genus: Genus Genus, Genus Genus: Genus Genus: Genus Genus, Genus Kayamonori, Genus Genus: Genus Genus: Genus Filiformis, Genus Turmo: Genus Tsurumo, Genus Anana: Genus Anastasia, Genus Suzume: Genus Musicum, Genus Missiscoma, Genus L. , Oni-kombu, Gohei-kombu, Naga-kombu, Pea-kombu, Ochimitsu-kombu, Toro-kombu genus: Toro-kombu, Antokume genus: Antokume, Kajime genus: Kajime, Tsuruarame, Kurome, Kikuishicombu genus: Kikuishikombu, Nejirekombu: Nekorecome genus, Kuroshimae : Nekoshikambu,
Alame: Alame, Ainuwakame: Ainuwakame, Chigaiso, Oniwakameezoishige: Ezoishige, Yabanemoku: Yanemoku, Rapamoku: Rapamoku, Jiromoku: Uganomok, Jiromoku, Hiemomoku, Hokumoku, Tamamomoku, Tamamomoku Akamoku, Shidamoku, Hondawara, Nejimoku, Narasamo, Mametarawara, Tatsukuri, Yasumatamoku, Umitrano, Oobamoku, Fushizushimoku, Hahakimoku, Togemoku, Jolemoku, Saw-moku, Obanoki-mimoku, Genus genus:
Bulukimo, genus cynomolgus: cynomolgus etc.).

Red algae (Russia genus: Ushikenori, Funorinoushike, Amanori: Asakusanori, Susabinori,
Genus Upirinori, Onimanori, Tasa, Filitasa, Benitasa, Rhodocorton: Milnoveni, Akebonomozuku: Akebonomozuku, Konahada: Haikonahada, Yogorekonahada, Aokonahada, Sea Species: Acer genus, Acer genus : Larch, Fusanori: Fusanori, Nisefusanori: Nisefusanori,
Sodegarami: Sodegarami, rattles: rattle, rattle rattle, hirohatamatamaki genus: hirohatamaitadaki, tamamaidaki genus: tamaitadaki, kagikenori genus: kaginori, kakenenori, tengusa: himetenugosa, haitenugosa, haitenugosa, haitenugusaugou, haitenuganugou, haitenuganugou, haitenugusaugou Genus: hibiroudo, himehibiroudo,
Genus Isommedogi: Genus Isomomedo, Genus genus: Genus medusa, Ryumonsou: Ryumonsou, Hera Ryumon, Nisekarekususa: Genus Nisekaregusa, Okitsubara: Obaokitsubara, Akaba: Akaba, Malvaacaba, Naminohana genus
Naminohana, coral genus: rattle, mushroom, shiogosagoromo, iwanokawa: ezukiiwanokawa, kainokawa: kainokawa, kaninote: kaninote, coral genus: coral, coral, mosquito mosquito , Tambanori, slippery,
Fudarak, Malbahudarak, Isonohana: Nuracusa,
Cranouraxa, Stinging skull, Hunting genus: Hunting ibis, Hunting genus: Hunting sword, Hunting genus: Chabo kintoki, Hunting ibis, Matsunori, Komenori, Tosakamatsu, Aedes pine, Fushikintoki, Tsunomukade, Nagakinotoki, Scutellariae, Osmanthus Nagaobabane: Nagaobabane,
Genus Funori: Hanafunori, Fukurofunori, Makunori, Kalexigusa: Kalexigusa, Sasamodoki: Hosobanotosakamaki, Hirohanokasakamuki, Yatsudegatatasakamoki, Kurosakamaki, Nezashinosakamukado, Yukasakanookado Modoki,
Genus genus, genus genus: quinohada, genus esculenta: esoteka, tsukasanori genus: enashikarimenia, ootsukasanori, hanagatakarimenija, honooo genus: honoo, genus genus: lyca genus, genus genus, genus nikonobenigo Susqueke Beni, Okamuragusa:
Yamadakusa, Mirin genus: Mirin, Hosobamilin, Tosakanori genus: Kikutosaka, Ezonameshi: Ezonameshi, Isomokka genus: Isomocca, Yukari genus: Yukari, Hosoyukari, Ibaranori genus: Ibaranori, Saidaibara, Tachiibara, Kagiibanodan, Kijinotho genus Isanthus, Atsubanori: Atsubanori,
Ogonori: Ogonori, Tsurusilamo, Shiramo, Ogogonori, Mizookonori, Kabanori, Beniogonori, Fucresinori, Murasakikabanori, Shinkaikabanori, Togekabanori, Kataogonori, Ryukyuogonori, Ryokyuogonori, Ryokyuogonori, Ryokyuogonori, Ryokyuogonori , Genus Asterina: Acacia serrata, Fushikrenori genus: Fushikrenori, Namiiwatake genus: Namiiwatake, scorpion genus: Scorpion genus, Occidental genus: Okutsunori, Simi genus: Itanisa, Simi, Harigane, Lotus japonicus
Lotus spiders, genus genus: Ikanoashi, Hosoibonori,
Nobonori, Black Snapper: Black Snapper, Red Snapper: Red Snapper, Hishbukuro: Hishbukuro, Madaragusa: Togemadara, Echimadara, Taoyagousou: Genus Ottawa, Scrophularia , Hirata goat: Hirata goat, Dulce: Dars, Masagoshibari, Anadalus, Uebagsa: Uebagsa, Benifukuronori: Benifukuronori, Fushinagi: Fushitsunagi, Himefushinagi,
Species of the genus Hirohafushi, Snapdragons: Snapdragons, Snapdragons, Species: igis, keiigis, crickets, nigiri, amixa, egonori:
Egonori, Futogigis, Saeda: Saeda, Chrysanthemum Genus: Chrysanthemum, Konohanori Family: Habutaenori, Konohanori, Suzushironori, Usbeni: Usveni, Hasuginu: Hasuginu, Nagakonohanori: Nagakosugino, Genus: suginanu Yareusbanori, Sujiusbanori, Hyusbanori, Usubanorimodoki: Usubanorimodoki, Ayanishiki: Ayanishiki, Ayaginu:
Ayaginu, Dassia genus: Enashidazia, Simadadia genus: Isohagi, Shimadada, Dazimodoki genus: Daziomomodoki, Itogusa genus: Moletogusa, Futoigusa, Macri genus: Macri, Yanaginori genus: Hanayanagi, Yuna, Sanaginori, Mosanyugina, Sasa, Rabbitum : Kurozozo, Kobusoso, Hanezozo, Sozonohana, Hanegusa: Hanegusa, Kanegusa, Kozanemo: Kozanemo, Isomurasaki, Hosokozanemo, Himegoge: Himegoke, Kurohimegoke, Hiodoshig, Hoshigusa Suginasugusa, Isobasho Genus: Isobasho, Fujimatsumo Genus: Fujimatsumo, Sawwort: Genus Hakezakinokawa, Kawamozuku: Kawamozuku, A Kawamozuku, Himekawamozuku,
Ideyugogome: Ideyugogome, Okitimozuku: Okitimozuku, Itogusa, Chinorimo: Chinorimo, Chisginori: Chisinori, etc.).

Wheel algae (Characta spp., Shiratamamo spp.,
Hossiturimo: Hossiturimo, Lichnotumnus, Flassula: Himegrass, Chaboflas, Tripella, etc., Yellow algae (Hikarimo: Hikarimo, etc.) and the like.

[0039] Materials derived from animal raw materials include a cockscomb extract, a cow or human placenta extract, a pig or cow extract from the stomach, duodenum or intestine, or a decomposition product thereof, and a pig or cow spleen extract. Product or its degradation product, extract of pig or cow brain tissue, water-soluble collagen, collagen derivative such as acylated collagen, collagen hydrolyzate, elastin, elastin hydrolyzate, water-soluble elastin derivative, keratin and its decomposition product Or a derivative thereof, a silk protein and its degradation product or a derivative thereof, a pig or bovine blood cell protein degradation product (globin peptide), a pig or bovine hemoglobin degradation product (hemin, hematin, heme, protohem, heme iron, etc.),
Milk, casein and its degradation products or their derivatives, skim milk powder and its degradation products or their derivatives, lactoferrin or its degradation products, chicken egg components, fish meat degradation products, nucleic acid-related substances (ribonucleic acid, deoxyribonucleic acid) and the like.

(11) Microbial culture metabolite Yeast metabolite, yeast extract, natto metabolite, natto extract, rice ferment extract, rice bran (red bran, white bran) ferment extract, euglena extract, raw milk or skim milk powder Lactic acid fermentation products, trehalose or derivatives thereof.

(12) α-hydroxy acids glycolic acid, citric acid, malic acid, tartaric acid, lactic acid and the like.

(13) Inorganic pigments: anhydrous silicic acid, magnesium silicate, talc, kaolin,
Bentonite, mica, titanium mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide,
Titanium oxide, calcium carbonate, magnesium carbonate, yellow iron oxide, red iron oxide, black iron oxide, gunjou, chromium oxide,
Chromium hydroxide, carbon black, calamine, etc.

(14) UV absorber / blocker Benzophenone derivatives (2-hydroxy-4-methoxybenzophenone, 2-hydroxy-4-methoxybenzophenone-
5-sulfonic acid, 2-hydroxy-4-methoxybenzophenone-5-sulfonic acid sodium salt, dihydroxydimethoxybenzophenone, sodium dihydroxydimethoxybenzophenone-sulfonic acid salt, 2,4-dihydroxybenzophenone, tetrahydroxybenzophenone, etc.), p-aminobenzoic acid Derivatives (paraaminobenzoic acid, ethyl paraaminobenzoate, glyceryl paraaminobenzoate, amyl paradimethylaminobenzoate, octyl paradimethylaminobenzoate, etc.), and methoxycinnamic acid derivatives (ethyl paramethoxycinnamate, isopropyl paramethoxycinnamate,
Octyl paramethoxycinnamate, 2-ethoxyethyl paramethoxycinnamate, sodium paramethoxycinnamate, potassium paramethoxycinnamate, mono-2-diparamethoxycinnamate
Glyceryl ethylhexanoate, etc.), salicylic acid derivatives (octyl salicylate, salicylphenyl, homomenthyl salicylate, dipropylene glycol salicylate, ethylene glycol salicylate, myristyl salicylate,
Methyl salicylate), anthranilic acid derivatives (methyl anthranilate), urocanic acid derivatives (urocanic acid, ethyl urocanate, etc.), coumarin derivatives, amino acid compounds, benzotriazole derivatives, tetrazole derivatives, imidazoline derivatives, pyrimidine derivatives, dioxane derivatives , Camphor derivative, furan derivative, pyrone derivative, nucleic acid derivative, allantoin derivative, nicotinic acid derivative, vitamin B6 derivative, umbelliferone, esculin, benzyl cinnamate, sinoxate, oxybenzone, dioxybenzone, octabenzone, slisobenzone, benzoresorcinol, arbutin Guaiazulene, shikonin, baicalin, baicalein, berberine, neoheliopan, escalol, zinc oxide, talc, kaolin and the like.

(15) Whitening agent p-aminobenzoic acid derivative, salicylic acid derivative, anthranilic acid derivative, coumarin derivative, amino acid compound,
Benzotriazole derivatives, tetrazole derivatives, imidazoline derivatives, pyrimidine derivatives, dioxane derivatives, camphor derivatives, furan derivatives, pyrone derivatives,
Nucleic acid derivatives, allantoin derivatives, nicotinic acid derivatives,
Vitamin C or its derivatives (vitamin C phosphate magnesium salt, vitamin C glucoside, etc.), vitamin E or its derivatives, kojic acid or its derivatives, oxybenzone, benzophenone, arbutin, guaiazulene, shikonin, baicalin, baicalein, berberine, placenta extract , Ellagic acid, lucinol and the like.

(16) Tyrosinase activity inhibitor Vitamin C or a derivative thereof (vitamin C phosphate ester magnesium salt, vitamin C glucoside or the like), hydroquinone or a derivative thereof (hydroquinone benzyl ether or the like), kojic acid or a derivative thereof, vitamin E or Derivatives thereof, N-acetyltyrosine or derivatives thereof, glutathione, hydrogen peroxide, zinc peroxide, placental extract, ellagic acid, arbutin, rucinol, silk extract, plant extracts (chamomile, mulberry, mulberry, waremokou, clara, mugwort, Honeysuckle, yellowfin, yellowtail, matsuhodo, adlay, dragonfly, hops, hawthorn, eucalyptus, achillea millefolium, altea, keihi, mankeishi,
Hamamelis, yamaguwa, life-prolonging grass, bellflower, toshishi, spouse, sun-dried, mako, senkyu, dokkatsu, psycho, boufu, hamaboufu, ougon, peony, peonies,
Gennoshoko, kakkon, licorice, five times child, aloe, ginger, safflower, green tea, black tea, asenyaku), etc.

(17) Melanin pigment reduction / decomposition substance Phenylmercury hexachlorophene, mercuric oxide, mercurous chloride, aqueous hydrogen peroxide, zinc peroxide, hydroquinone or a derivative thereof (hydroquinone benzyl ether)
etc.

(18) Turnover promoting action / cell activator hydroquinone, lactic acid bacteria extract, placenta extract, reishi extract, vitamin A, vitamin E, allantoin, spleen extract, thymus extract, yeast extract, fermented milk extract, plant extract (Aloe, Ogon, Horsetail, Gentian, Burdock,
Sicon, carrot, hamamelis, hop, yokunin,
Odrich, Assemble, Touki, Calendula officinalis, Amacha, Hypericum perforatum, Cucumber, Periwinkle,
Rosemary, parsley) etc.

(19) Astringent succinic acid, allantoin, zinc chloride, zinc sulfate, zinc oxide, calamine, zinc p-phenolsulfonate, potassium aluminum sulfate, resorcinol, ferric chloride, tannic acid (including catechin compound) etc.

(20) Active oxygen scavenger SOD, catalase, glutathione peroxidase and the like.

(21) Antioxidants Vitamin C or a salt thereof, stearic acid ester, vitamin E or a derivative thereof, nordihydroguaselethenoic acid, butylhydroxytoluene (BHT), butylhydroxyanisole (BHA), hydroxytyrosol, Parahydroxyanisole, propyl gallate, sesamol,
Sesamolin, gossypol, etc.

(22) Lipid peroxide production inhibitor β-carotene, plant extract (cultured sesame cells, amateur,
Hypericum, Hamamelis, Clove, Melissa, Emisoso, Birch, Salvia, Rosemary, Minami Tenji, Agetsu, Ginkgo, Green Tea) and the like.

(23) Anti-inflammatory agent Ictamole, indomethacin, kaolin, salicylic acid, sodium salicylate, methyl salicylate, acetylsalicylic acid, diphenhydramine hydrochloride, d-camphor,
dl-camphor, hydrocortisone, guaiazulene, camazulene, chlorpheniramine maleate, glycyrrhizic acid or its salt, glycyrrhetinic acid or its salt, licorice extract, sicon extract, age extract, propolis and the like.

(24) Humidifier Glycerin, propylene glycol, 1,3-butylene glycol, hyaluronic acid or its salt, polyethylene glycol, chondroitin sulfate or its salt, water-soluble chitin or chitosan derivative, pyrrolidone carboxylic acid or its salt, lactic acid Sodium, Mini Sasanishiki extract, Bacillus natto metabolite, Bacillus natto extract, Loofah water, Birch (birch) or Akamatsu sap

(25) Inhibitors of elastase activity diisopropyl fluorophosphate, plant extracts (Ougon, Hypericum perforatum, Clara, mulberry leaves, calyx, gennoshoko, comfrey, salvia, elderberry, bollige, botanpi), seaweed extracts and the like.

(26) Peripheral vascular blood flow promoter Vitamin E or a derivative thereof, assembly extract, garlic extract, carrot extract, aloe extract, gentian extract, touki extract, cepharanthin and the like.

(27) Stimulants Pepper tincture, capsaicin, vanylamide nonylate, tincture tincture, ginger tincture, peppermint oil, l-menthol, camphor and the like.

(28) Metabolic activators Photosensitizer 301, hinokitiol, pantothenic acid or derivatives thereof, allantoin, placental extract, biotin and the like.

(29) Flavors Natural animal flavors such as musk, civet, castorium, ambergris, anise essential oil, angelica essential oil, ylang-ylang essential oil, iris essential oil, fennel essential oil, orange essential oil, cananga essential oil, caraway essential oil, cardamom essential oil, Guayakwood essential oil, cumin essential oil, black letter essential oil, cinnamon essential oil, cinnamon essential oil, geranium essential oil, copaiba balsam essential oil, coriandel essential oil, perilla essential oil, cedarwood essential oil, citronella essential oil, jasmine essential oil, ginger grass essential oil, cedar essential oil, spearmint essential oil, western Mentha essential oil, Greater Fengxiang essential oil, Tuberose essential oil, T-shaped essential oil, Orange flower essential oil, Winter green essential oil, Truval sam essential oil, Batturi essential oil, Rose essential oil, Palmarosa essential oil, Cypress essential oil, Hiba essential oil,
Sandalwood essential oil, Petit glen essential oil, Bay essential oil, Vetiva essential oil, Bergamot essential oil, Peruvian balsam essential oil, Boad rose essential oil, Camphor essential oil, Mandarin essential oil, Eucalyptus essential oil, Lime essential oil, Lavender essential oil, Linaloe essential oil, Lemongrass essential oil, Lemon essential oil, Rosemary Vegetable flavors such as essential oils and Japanese peppermint essential oils, and other synthetic flavors.

(30) Dyes / colorants Red cabbage dye, red rice dye, madder dye, anato dye, squid dye, turmeric dye, orange dye, krill dye, persimmon dye, caramel, gold, silver, gardenia dye, corn dye , Onion pigment, tamarind pigment, spirulina pigment, buckwheat whole plant pigment, cherry pigment, laver pigment, hibiscus pigment, grape juice pigment, marigold pigment, purple potato pigment, purple yam pigment, lac pigment, rutin and the like.

(31) Sweeteners Sugar, sweet tea, fructose, arabinose, galactose, xylose, stevia, mannose, maltose, honey, glucose, miraculin, monelin, licorice extract, larkane extract and the like.

(32) Nutrition enhancer Shell calcined calcium, cyanocorabamine, yeast, wheat germ, egg yolk powder, hemicellulose, heme iron and the like.

(33) Dairy products Milk, cheese, fresh cream, butter, margarine, milk powder, whey, condensed milk and the like.

In addition, humectants, sequestering agents, pH
Conditioning agents, chelating agents, preservatives / antibiotics, cooling agents, stabilizers, emulsifiers, animal / vegetable proteins and their degradation products, animal / vegetable polysaccharides and their degradation products, animal / vegetable glycoproteins and their Decomposition products, blood flow promoters, anti-inflammatory agents / anti-allergic agents, cell activators, foaming agents, thickeners, deodorants / deodorants, bitters, seasonings, enzymes, etc. Various additive or synergistic effects can be expected.

[0064]

EXAMPLES Production Example 1: Milk protein hydrolyzate Commercial milk (1 ton) was put into a reaction kettle, and the milk protein was sterilized by raising the temperature to about 90 ° C. Then, the mixture is cooled to 37 ° C., and a protease is allowed to act on the mixture. The enzyme used is
Trypsin, chymotrypsin, pepsin, rennin, pancreatin, elastase, carboxypeptidase, aminopeptidase, dipeptidase, papain,
Alcalase or the like can be used. Here, trypsin is used. Trypsin 1 is added to the milk material 40 to 50 at a ratio of 1 and the mixture is aseptically hydrolyzed at 37 ° C. and pH 6 to 8 for 8 hours. Thereafter, the temperature of the system is raised to 80 ° C. or higher to inactivate the enzyme, and then the precipitate is removed by centrifugation. The solution part thus obtained was lyophilized to about 8
kg of milk protein hydrolyzate powder (molecular weight 300-8,000) was obtained.

[0065]

Production Example 2: Silk protein hydrolyzate After heating 20 kg of silk at a temperature of 80 ° C. or higher and sterilizing by heating, this was added to a highly concentrated salt solution of lithium bromide, magnesium nitrate and calcium chloride (100 kg of a saturated solution). Heat and dissolve
Next, the protein is desalted by dialysis, reverse osmosis or the like, and then a protease is allowed to act. As a proteolytic enzyme to be used, trypsin is allowed to act on silk 40 at a ratio of 1 to 37 ° C. and pH 7. At that time, it is aseptically decomposed for 24 hours. Thereafter, the temperature was raised to 80 ° C. or higher to inactivate the enzyme, and the precipitate was removed by centrifugation to remove the silk protein hydrolyzate (molecular weight: 300
~ 10,000). This is dried (spray-dried) to obtain about 10 kg of powder.

[0066]

[Example] Production Example 3: Placenta extract Bovine placenta is pulverized, and a solution hydrolyzed with a protease (for example, 0.6 L of Alcalase manufactured by Novo) is defatted, purified, treated with an excipient, and dried.

[0067]

EXAMPLE Evaluation of immunostimulatory activity Ten C57BL male mice (about 20 g) were placed in one cage (relative ratio W: D: H = 1.0: 1.0: 1.0).
Keep for a week. After 3 weeks, cages (relative ratio W: D:
H = 0.5: 0.5: 1.0) and put the mice under overcrowding conditions to apply stress. One week later, 0.15 mL of sheep red blood cell suspension is intraperitoneally administered to sensitize. Six days after sensitization, 0.04 mL of a sheep erythrocyte solution is injected subcutaneously into the hind footpad to elicit. Twenty-four hours later, the thickness of the hind footpad is measured. During the test, the test substance is dissolved in water and supplied automatically. In addition, a control group bred in water and loaded with overcrowding stress, and an untreated group bred in water and not loaded with overcrowding stress are provided, and measurement is similarly performed.

The results are shown in the following tables (Tables 1 to 3). Table 1 shows the active ingredient according to the present invention, Table 2 shows the auxiliary ingredient, and Tables 3 and 4 show the immunity-activating effect of the combined use of the active ingredient and the auxiliary ingredient. That is, the untreated value is the value shown by the non-stressed mouse, and the immune response of the mouse decreases to the control value due to the overcrowded environmental stress. On the other hand, the sample has the effect of strengthening the immune function predominantly and adjusting its homeostatic balance (Table 1). Furthermore, it was confirmed that the combined use of the auxiliary component with the 1/10 amount of the active ingredient clearly improved the effect (Table 3).

[0069]

[Table 1]

[0070]

[Table 2]

[0071]

[Table 3]

[0072]

[Table 4]

[0073]

[Example] Safety (Acute toxicity test) Using a milk protein hydrolyzate, silk protein hydrolyzate and placenta extract prepared according to the production example as samples, dd was fasted for 4 hours before the test.
Y-series mice (5 mice per group, body weight of about 30 g) were orally administered in an amount of 2,000 mg / kg body weight as solids, and the appearance and degree of toxicity symptoms were observed over time. As a result, in each sample, no abnormality was observed in all the mice for 14 days, and there was no abnormality in the dissection result.
LD50 was determined to be 2,000 mg / kg or more.

[0074]

EXAMPLE Effect on Skin Viscoelasticity In order to examine the effect on skin viscoelasticity, a food and drink test was conducted with volunteers (18-36-year-old female, 19). Take 1 g of the sample (in the case of mixing, equal amounts of each mixture) at a frequency of 3 times / day after each meal, and continue for 1 month. The viscoelasticity of the skin area under the right eye of the volunteer is measured with a skin viscoelasticity meter (CUTOMETER SEM 474 manufactured by Eurotech Japan), and compared with the skin value before the start of the eating and drinking test. The measurement condition is Modo1, the operation of applying a negative pressure of 500 mb for 5 seconds is repeated twice at intervals of 5 seconds, the operation is performed three times, and the average value is calculated. The degree of plasticity and elasticity were determined according to the usual method.

The results are shown in the following tables (Tables 5 and 6). As shown in Tables 5 and 6, it is shown that the plasticity decreases and the elasticity increases. Improvement of elasticity was observed in all subjects, and it was considered that the skin had firming effect.

[0076]

[Table 5]

[0077]

[Table 6]

[0078]

EXAMPLES Application Examples (Health Food Tablets) A composition containing the following additives was tabletted into tablets. % By weight 1. Dry powder of milk protein hydrolyzate 20 2. Dry powder of silk protein hydrolyzate 20 Propolis extract dried powder 5 4. Agaricus extract powder 5 5. Lactose 25 6. Corn starch 20 7. Hydroxypropyl cellulose 4 8. Magnesium stearate 1

[0079]

EXAMPLES Application Examples (Healthy Drink) A health drink containing the following additives was produced. % By weight 1. Silk protein hydrolyzate solution (10%) 10 2. Carboxymethylated β-1,3 glucan 0.2 Citric acid 0.1 4. dl-malic acid 0.15. D-sorbitol solution (70%) 10 6. Appropriate amount of fragrance 7. Purified water residue

[0080]

EXAMPLES Application Examples (Juice Drink) A juice drink containing the following additives was produced. Weight g 1. Milk protein hydrolyzate solution (10%) 10 2. Placenta extract solution (10%) 10 3. Glucose solution 20 4. Grapefruit juice 30 5. Orange juice 30 6. Appropriate amount of fragrance 7. Sour seasoning appropriate amount

[0081]

Example: Use test for volunteers suffering from stress-related discomfort Symptoms of 20 patients aged 18 to 65 who suffer from anorexia, insomnia, stiff shoulders, and headache caused by stress, every meal for 3 months (3 times a day) ), The tablet of the example (equivalent to 3 g / day) was taken orally, and a test was carried out to evaluate the effect on the self-discomfort symptoms. The evaluation was performed according to the following criteria. Efficacy: Anorexia, insomnia, stiff shoulders, and headache due to stress were improved. Somewhat effective: Anorexia, insomnia, stiff shoulders, and headache due to stress were slightly improved. Ineffective: Anorexia, insomnia, stiff shoulders and headache due to stress remain unchanged from before use.

The results are as shown in Table 7. It was confirmed that taking the immunostimulating food of the present invention was effective against anorexia, insomnia, stiff shoulders, and headache caused by stress. The numbers in the table represent the number of people.

[Table 7]

[0083]

EFFECTS OF THE INVENTION The food for immunostimulation according to the present invention enhances immunity, regulates its homeostasis, creates a constitution that is less susceptible to illness, and has anorexia, insomnia, stiff shoulders, headache, etc. caused by stress. Improves skin and shows beauty effect (recovery of skin elasticity).

────────────────────────────────────────────────── ───

[Procedure amendment]

[Submission date] February 22, 2001 (2001.2.2)
2)

[Procedure amendment 1]

[Document name to be amended] Statement

[Correction target item name] 0018

[Correction method] Change

[Correction contents]

The foodstuff for immunostimulation of the present invention may optionally contain, in addition to the above-mentioned essential components, any of the following components and additives within a range that does not impair the effects of the present invention. The content in the preparation is not particularly limited, but is usually preferably 0.0001 to 50%.

──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. ⁷ Identification symbol FI Theme coat ゛ (Reference) A23L 1/229 A23L 1/229 Z 1/28 1/28 Z F term (Reference) 4B018 MD20 MD33 MD44 MD69 MD71 MD78 MD82 ME14 MF01 MF10 MF12 4B041 LD06 LH16 LK14 LK35 LK37 LK39 LK42 LP05 4B047 LB03 LB06 LB09 LG19 LG26 LG35 LG37 LG50 LG51 LP18

Claims (2)

[Claims] (1) A food for immunostimulation comprising at least one selected from milk protein hydrolyzate, silk protein hydrolyzate and placenta extract. 2. An immunostimulating food product comprising the following essential components (1) and (2): (1) One or more selected from milk protein hydrolyzate, silk protein hydrolyzate, and placenta extract. (2) at least one selected from nucleic acids, β-1,3 glucan or derivatives thereof, propolis extracts and agaricus extracts.