JP2007131579A - Physiologically active composition and method for producing the same - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a physiologically active composition which arranges the reinforcement of self immunological functions and homeostasis balance to maintain health and has such action to make physical conditions hardly suffering from diseases. <P>SOLUTION: This physiologically active composition comprises a plant extract obtained by steaming a plant at 120 to 250°C and then extracting the steamed plant with a solvent. This physiologically active composition, wherein the plant is a gramineous plant. A method for producing a physiologically active composition comprises steaming a plant at 120 to 250°C and then extracting the steamed plant with a solvent. The method for producing the physiologically active composition, wherein the plant is a gramineous plant. <P>COPYRIGHT: (C)2007,JPO&INPIT

Description

  The present invention has an excellent immunostimulatory action that reinforces and restores immune ability that has been strengthened or reduced, as well as an antimutagenic action, an antibacterial action, an antioxidant action, an anticancer action, an intestinal environment improving action, and a reduction in neutral fat. The present invention relates to a bioactive composition having an action and the like.

  Western medicine has played a major role in the treatment of infectious diseases. However, on the other hand, dealing with such infectious diseases and adult diseases in preventive medicine is becoming more and more important in the coming aging society.

  Living organisms are exposed to the invasion of viruses, microorganisms, and the like from the outside, and live in a situation where foreign self-substances (for example, cancer, self-dead cells, etc.) appear. We can survive in this situation because we have a mechanism to eliminate such foreign substances and defend ourselves. Therefore, such deterioration and abnormality of the body defense ability (immunity ability) may cause various diseases such as bronchial asthma, allergic diseases, chemical sensitivity, rheumatoid arthritis, autoimmune diseases, chronic fatigue syndrome, viruses and bacteria. become. Generally, stress, infectious diseases (measles, influenza, AIDS, etc.), chemical exposure, etc. are considered to be the factors that lead to such a decline in immunity. In addition, immunity decreases with age.

  The in vivo immune system plays an important role in the defense against tumors and infections by microorganisms such as bacteria, yeasts, molds and viruses, and the main mechanism is that T lymphocytes and B lymphocytes are these microorganisms. Or stimulating tumors by recognizing them through the antigen receptor, activating antigen-specifically and enhancing their ability to eliminate these foreign substances. In a living body that is constantly exposed to microorganisms and whose cells are mutated, such activation of T lymphocytes and B lymphocytes always occurs. In addition to antigen-specific activation, activation of T lymphocytes and B lymphocytes includes non-antigen-specific activation via receptors that recognize bacterial components, lectins, and the like. In both antigen-specific and non-antigen-specific activation, the activation of T lymphocytes and B lymphocytes is further increased when a stimulus from the other, ie, costimulation is applied.

Current immunostimulators enhance the body's defense mechanisms against microbial infections and tumors by non-specifically activating immunocompetent cells, but few are satisfactory in effectiveness. In addition, since these immunostimulators activate immunocompetent cells alone, they may induce immune responses that are undesirable for the living body, and may cause side effects such as exacerbation of various allergic reactions.
On the other hand, administration of drugs such as various antibiotics is carried out as a coping therapy for diseases affected by decreased immunity, for example, infectious diseases. However, problems such as the emergence of drug-resistant bacteria have arisen as a result of the easy use of such drugs. From these reflections, searching for substances that can maintain health by improving the immunity of the living body has been widely conducted.

  In addition, the search for substances that act on the genetic material DNA of cells to damage and cause mutations and suppress mutagens that cause cancer has been widely conducted, and various antimutagenic agents have been found. Antimutagenic agents are expected to prevent DNA damage, have anticancer effects, and are widely used to prevent cellular aging. Ascorbic acid having a reducing action is widely known as an antimutagenic agent (Osawa, T. et al .: Biochem, Biophysic, Res. Commun., 95; 835, 1980). Searches from plants are also widely conducted, and it is known that polyphenols, which are plant components, also have an antimutagenic effect using the Ames test using Salmonella.

  On the other hand, in our eating habits, what has been seen as a problem in health management in recent years is the effect of active oxygen generated in the living body, which seems to be due to various synthetic food additives and residual agricultural chemicals. Living organisms work to remove this harmful active oxygen by enzymes, etc., but when this removal function decreases due to aging etc., even normal cells and organs may be damaged, resulting in various diseases and There are concerns about aging of the organization. For these reasons, the usefulness of foods containing a large amount of components having an antioxidant action, such as vitamin E and vitamin C, has been pointed out.

  Recently, interest in adult diseases, in particular, cancer, stroke, heart disease, etc. has been greatly increased, and daily eating habits are being reviewed. For example, high blood pressure is regarded as a risk factor for cardiovascular diseases, and it is said that hypertensives are 2 to 3 times more likely to have a stroke or heart disease than normal blood pressure. In addition, obesity in middle-aged people and the accompanying increase in neutral fat and cholesterol are at high risk of causing arteriosclerosis and myocardial infarction. Avoid excessive drinking, irritating seasonings, high-fat foods, etc. as much as possible and have a good balance. It is said that it is important to keep a meal.

  In addition, the air pollution and ozone layer destruction, which are taken out as familiar environmental problems, are also a cause of damaging health. In particular, the destruction of the ozone layer increases harmful ultraviolet rays and causes serious problems such as spots, freckles, and skin cancer. For this reason, some regions encourage clothing or sunglasses that do not expose the skin as much as possible and call for protection.

  On the other hand, recently, it has become increasingly clear that free radicals cause various diseases (Pharmacia Vol. 29, No. 9, p971-1002, published in 1993). The relationship with anti-cancer is well known. In recent years, air pollution has been cited as one of the reasons why the prevalence of allergic diseases in the population has increased. For example, in the case of automobile exhaust, black soot in it emits a large amount of active oxygen, It has recently been found that this causes allergic symptoms. If active oxygen is the direct cause of allergic disease, an active oxygen scavenger may be administered, but the relationship between patients with allergic disease and foods and drinks that have active oxygen scavenging activity will be studied over a long period of time. As a matter of course, there are few papers that have been made.

  Therefore, it is safe if it contains a highly stable antioxidant active ingredient in the usual use dose and has a taste like tea and it is usually taken as food and drink. It can be said that there is no problem in sex and is ideal as a functional food.

  There are various health teas on the market, but most of them are folklore in terms of their functional usefulness. Even though it is a food and drink, health tea is more than just an effect to prevent health, maintain health, etc. Few things can be said to be based on certain scientific facts that this is better than beverages.

Japanese Patent Laid-Open No. 10-203994 Japanese Unexamined Patent Publication No. 07-184545

  In order to maintain health under such circumstances, it is important to strengthen the self-immune function and create a constitution that maintains homeostasis and is unlikely to cause illness. The present invention is to provide a physiologically active composition having such effects.

  The present invention relates to the physiologically active composition as described above, wherein the extract component of the gramineous plant is obtained by steaming the leaves and / or straw of the gramineous plant and then extracting with an aqueous solvent.

  In the present invention, steaming steams gramineous leaves and / or straw at 120-250 ° C. for 30 seconds to 1 hour with dry steam whose dryness is improved by a pressure reducing valve and a gas-liquid separator. It is related with the said bioactive composition.

  Further, the present invention provides the above physiological activity, wherein steaming is performed by steaming grass and / or straw and water at a steaming temperature of 120 to 250 ° C. under a weight mixing ratio of 100: 50 to 100: 150. Relates to the composition.

  Moreover, this invention relates to the said bioactive composition whose aqueous-solvent extraction temperature is 100-150 degreeC.

  The physiologically active composition of the first invention of the present invention is good because it contains a plant extract obtained by solvent extraction after steaming a plant at 120 to 250 ° C.

  The physiologically active composition of the second invention of the present invention has a better effect because the plant is a gramineous plant.

  In the method for producing a physiologically active composition of the third invention of the present invention, the plant is steamed at 120 to 250 ° C. and then extracted with a solvent, so that effective components and amounts can be obtained.

  In the method for producing a physiologically active composition according to the fourth aspect of the present invention, a better extract can be obtained because the plant is a gramineous plant.

(plant)
Although there is no limitation in the plant used by this invention, a Gramineae plant is preferable.
(Gramineae plant definition)
The grass family is a family of angiosperm monocotyledons to which approximately 600 genera and 10,000 species belong, and includes rice, wheat, barley, corn, sugarcane, bamboo, and sugar.
The representative genera of the subfamily of the family Gramineae include the genus Bamboo genus and Sasa genus, the subfamily Gramineae genus, the wheat genus Subfamily genus Wheat, the zelkova subfamily Shiba genus, the genus Subfamily genus, and the millet genus Millet genus. , Sorghum and Susuki. Among these, plants of the bamboo family and the millet family are preferable.

  Examples of the Poaceae Bamboo genus, Horaichiku, Tansuichiku, Himalayan Calamus, Indokaramus, Kanchiku, Kundsware, Chusquare, Ottaea, Yadatake, Medaka, Sasa, Kumasasa, Azumazasa Is preferred. Of these, Sasa genus and Kumaza genus are desirable. Specific examples include Okuyamamasasa, Gotenbazasa, Katsuragisa, Suzuda, Koyachiku, Phyris, Kumasu, Kizujisuzu, Kesuzu, Unzensa, Miyama Kazasa, Nemagaridake, Kinmeishishima, Konimanegagari, Shimofurinemagariboshigae, Shikibunemagaribachimakiboshigakiboshigakigakiboshigakigakiboshimakiboshigakiboshigakibakigaki This is limited to the following species: However, in the present invention, all generally called bamboo or bamboo are effective. In particular, what is generally called Kumazasa, such as Kumazasa, Kumizasa, Chishimasasa, etc., which can remove whitish wrinkles around the leaves is preferable.

  Examples of the Gramineae subfamily include millet genus such as sugar cane and sorghum genus such as corn.

The plant part used in the present invention may be any of a leaf, a stem and a cocoon. In the case of gramineous plants, leaves and stems, particularly in the case of Sasa, leaves and straws are preferably used.
In addition, raw leaves before drying can be used, but sufficiently dried ones are preferable. In the case of gramineous plants, a water content of 10% by weight or less is preferred with respect to the total weight of leaves and / or stems and / or straw. If it is more than that, the ingredients may be altered by spoilage and fermentation.
The shape of the plant during steaming is preferably a fragmented one. The size is preferably 0.5 to 20 mm, and if it is smaller than 0.5 mm, solid-liquid separation after extraction may be difficult, and if it is larger than 20 mm, the extraction efficiency may be deteriorated.

(Steaming process)
Any apparatus can be used as the apparatus used in the steaming process of the present invention as long as it can withstand high temperatures and high pressures. Hereinafter, the case where the gramineous plant is Sasa leaves will be described. In the case of a part other than leaves or other plant bodies, it can be similarly performed.

The cooking temperature of the plant is 120-250 ° C. Preferably it is 150-220 degreeC, More preferably, it is 160-190 degreeC. Below 120 ° C., the cell wall is hardly destroyed by the decomposition of hemicellulose in the straw, except when the raw material is a dry leaf, so that a desired component cannot be sufficiently extracted. When the temperature is higher than 250 ° C., the active ingredient is decomposed so that the medicinal effect may be impaired.
The cooking time is preferably between 30 seconds and 1 hour, more preferably between 5 minutes and 20 minutes. If it is shorter than 30 seconds, the hydrolysis of the hemicellulose is insufficient and the amount of the extracted component is reduced. If it is longer than 1 hour, the obtained component is decomposed, so that the amount of the obtained component is reduced.

When the plant raw material is a dried product (water content is 10% by weight or less), water can be added during steaming. At this time, it is preferable to steam the plant raw material and water at a mixing ratio of 100: 50 to 150.
If it is less than 100: 50, the amount of water required for hydrolysis of hemicellulose or the like may be insufficient. When the ratio is more than 100: 150, the steam does not reach the plant raw material, the cell wall is hardly destroyed, and a desired component may not be sufficiently extracted.
Moreover, as for the cooking temperature in case a plant raw material is a dried material, 120-170 degreeC is the most preferable. If it is less than 120 degreeC, destruction of the cell wall by decomposition | disassembly of the hemicellulose in a plant raw material does not occur easily, and a desired component may not fully be extracted. When the temperature is higher than 170 ° C., decomposition of active ingredients such as coumaric acid becomes severe and the drug efficacy may be impaired.
The cooking time is preferably between 30 seconds and 1 hour, more preferably between 5 minutes and 20 minutes. If the time is shorter than 30 seconds, the hydrolysis of hemicellulose may be insufficient and the amount of the extracted component may be reduced. If the time is longer than 1 hour, the obtained component may be decomposed and the amount of the extracted component may be reduced.

  The steam used for steaming is preferably steam from an apparatus provided with a steam / water separator and a pressure reducing valve in the middle of a steam pipe connecting a steam generating source such as a boiler and the steaming apparatus. By using the apparatus, the dryness of the steam is improved. As the degree of dryness is high and the amount of condensate of steam called drain is small, it is possible to efficiently extract a specific component and appropriately control the extraction amount of sugars. Since there is no simple measurement method for the dryness of the vapor, it is difficult to directly measure it with a normal manufacturing apparatus. Therefore, in order to obtain dry steam, it is preferable to use a gas-liquid separator that has been confirmed to be able to achieve a dryness of 99% or more by a dryness measuring system owned by a gas-liquid separator manufacturer or the like. There are a centrifugal type and a baffle type as the gas-liquid separator, but the baffle type is preferable because it can stably achieve a high dryness regardless of the amount of steam.

  If air remains in the steaming apparatus during steaming, contact between saturated steam and bamboo leaves or straw is hindered, resulting in insufficient steaming and reduced extraction efficiency. As a means for avoiding the remaining of the air, it is only necessary to introduce steam with the manual or automatic air vent valve opened and to expel the air from the cooking apparatus. The vapor temperature for expelling air may be 80 to 200 ° C., preferably 100 to 180 ° C., and the time for expelling air is 0.5 to 60 minutes.

  When releasing the pressurization after completion of cooking, it is preferable to open the apparatus at once to bring the pressure to atmospheric pressure, since the destruction of the tissue of the sasa leaves or straw will progress further and the extraction efficiency will improve.

(Solvent extraction process)
In the present invention, the plant can be obtained by cooking and then immersing it in an extraction solvent such as water or alcohol with a solvent. It can also be obtained as a commercial product. The grass plant extract can be appropriately diluted with water or the like.

  The method for obtaining the plant body extract of the grass family used in the present invention is not particularly limited. For example, JP 06-197800 A, JP 11-199502 A, JP 2002-322079 A, The methods described in Japanese Patent Application No. 2004-222710 and Japanese Patent Application No. 2004-242239 can be used.

  In the extraction method using an aqueous solvent, after the cooking, the aqueous solvent is added while maintaining the cooling or temperature, and the steamed salmon is cooked in the aqueous solvent at room temperature to 180 ° C, preferably 100 to 150 ° C. Soak the leaves. The temperature during extraction is most preferably 100 to 150 ° C. When the temperature is lower than 100 ° C., the extraction efficiency is lowered, and when the temperature is higher than 150 ° C., the active ingredient is decomposed so that the amount of components obtained is reduced. The time for performing the extraction is preferably between 10 minutes and 1 hour, more preferably between 15 minutes and 30 minutes, and when it is shorter than 10 minutes, the elution amount is reduced and the extraction efficiency is lowered, and when it is longer than 1 hour, it is obtained. Decomposition of the components occurs and the amount of components obtained is also reduced.

  The solvent used in the present invention is preferably an alcohol such as methanol or ethanol, a water-soluble solvent such as acetone, an aqueous solution thereof, or water. Moreover, when permitted by the use of the plant extract, the extraction efficiency can be increased by using an alkaline aqueous solution. As the alkaline aqueous solution, sodium hydroxide, potassium hydroxide, sodium carbonate, sodium hydrogen carbonate, ammonium hydrogen carbonate and the like are preferable.

  In the present invention, the steaming step and the extraction step may be repeatedly performed without departing from the gist of the present invention. In addition, other steps such as a holding step may be provided before and after each step.

(Plant extract)
The components of the plant extract obtained in the present invention are complicated and not necessarily clear, but each contains 0.5 mg / g or more of phenylpropanoid, acetic acid, formic acid and 3-hydroxypyridine, and the heating residue is 40-60. It is preferable that the content of the saccharide when adjusted to wt% is 50 mg / g or less. The phenylpropanoid is preferably at least one compound selected from coumaric acid, ferulic acid, caffeic acid, and vanillin.

(Form of plant extract when added to bioactive composition)
The bioactive composition of the present invention includes a plant extract. The form of the plant extract to be included in the physiologically active composition is not particularly limited, and it may be a solution or a solid, or may be a mixture with other compounds. In the case of a mixture, it may be processed by addition of spray drying, freeze drying, dextrin and the like. Further, it may be subjected to a selection process such as filtration, column purification, and solvent washing.

  For example, additives generally used for granulation, such as excipients, binders, disintegrants, lubricants, talc, and fluidity improvers may be used. For example, starch, lactose, crystalline cellulose such as sodium mannitol carboxymethylcellulose, hydroxypropylcellulose, light anhydrous silicic acid such as carboxymethylcellulose calcium and magnesium stearate, cellulose acetate, chitosan, calcium alginate, calcium alginate, defatted rice sugar, Oil cake, soy flour, wheat flour, silicic anhydride, bran, rice bran flour, calcium carbonate, milk calcium, magnesium oxide, sodium bicarbonate, crystalline cellulose, starch, brewer's yeast, sugar, reduced lactose, vegetable oil, etc. By combining and prescribing, it can be produced as a tablet, capsule, granule or the like.

(Estimated action)
Regarding the extract components of gramineous plants, for example, bamboo or camellia plants, it is presumed that they contain a lot of lignin, cellulose, hemicellulose, polysaccharides and polyphenols, and the components are complicated and not always clear.

Extracts of gramineous plants, for example, bamboo or cocoon plants are monosaccharides mainly composed of pentose (pentose) such as xylose and arabinose, hexose (hexose) such as galactose and glucose, and the like. It is known to contain polysaccharides such as xylo-oligosaccharide and arabino-oligosaccharide polymerized from them, or arabinoxylan in which xylose and arabinose are intricately crosslinked.
Further, it is known that a large amount of phenylpropanoid polyphenols such as coumaric acid, ferulic acid, caffeic acid, and vanillin are contained in the grass family when compared with other plants. In addition to these, organic acids such as acetic acid, formic acid, propionic acid, and lactic acid, amino acids, nitrogen-containing ring compounds such as 3-hydroxypyridine, and the like are also included in a large amount.
In particular, the yield of these compounds is remarkably improved by passing through the above-described cooking step.

Although not elucidated biologically or biochemically, the bioactive composition of the present invention contains a gramineous plant extract component, so that these saccharides, polyphenols, or organic acids can activate biological substances. It is presumed that it contributes to the improvement of the body environment of living organisms and the improvement of health.
Bacteria in the intestine have useful aspects such as digestion and promotion of absorption of ingested food, and harmful aspects that produce spoilage substances such as ammonia and indole in the intestine. However, the generation of harmful substances by spoilage bacteria by amino acids that should be used by the host has an adverse effect not only on the loss of nutrients but also on the health and growth of livestock.

  Thus, by ingesting a bioactive composition containing an extract of a gramineous plant, for example, a Gramineae subfamily, it contributes to maintaining the health of the organism by promoting the increase of useful microorganisms in the intestines, and As the number of useful microorganisms increases, the growth of harmful microorganisms is suppressed, and the production of malodorous substances such as ammonia, hydrogen sulfide, and amines produced by harmful microorganisms is also suppressed. Be expected.

  The bioactive composition according to the present invention can be used as it is, but if necessary, used in pharmaceuticals, quasi-drugs, cosmetics, bath preparations, foods, beverages and the like as shown below. From among the various components and additives that have been used, those suitable for the application can be arbitrarily selected and used in combination to provide easy-to-use product forms.

  Various fats and oils Avocado oil, almond oil, fennel oil, sesame oil, olive oil, orange oil, orange raffer oil, sesame oil, cocoa butter, chamomile oil, carrot oil, cucumber oil, beef tallow, beef tallow fatty acid, cucuin nut oil, safflower oil , Soybean oil, camellia oil, corn oil, rapeseed oil, persic oil, castor oil, cottonseed oil, peanut oil, turtle oil, mink oil, egg yolk oil, cocoa butter, palm oil, palm kernel oil, owl, palm oil, beef tallow, Pork fat, milk fat, or hydrogenated products (hardened oil, etc.) of these fats and oils.

  Wax beeswax, carnauba wax, whale wax, lanolin, liquid lanolin, reduced lanolin, hard lanolin, candelilla wax, montan wax, shellac wax, etc.

  Mineral oil liquid paraffin, petrolatum, paraffin, ozokelide, ceresin, micro-cristan wax, squalene, squalane, pristane, etc.

  Fatty acids: natural fatty acids such as lauric acid, myristic acid, palmitic acid, stearic acid, behenic acid, oleic acid, 12-hydroxystearic acid, undecylenic acid, tall oil, lanolin fatty acid, isononanoic acid, caproic acid, 2-ethylbutanoic acid, Synthetic fatty acids such as isopentanoic acid, 2-methylpentanoic acid, 2-ethylhexanoic acid and isopentanoic acid.

  Alcohols: natural alcohols such as ethanol, isopyropanol, lauryl alcohol, cetanol, stearyl alcohol, oleyl alcohol, lanolin alcohol, cholesterol, phytosterol, and synthetic alcohols such as 2-hexyldecanol, isostearyl alcohol, and 2-octyldodecanol.

Polyhydric alcohols ethylene oxide, ethylene glycol, diethylene glycol, triethylene glycol, ethylene glycol monoethyl ether, ethylene glycol monobutyl ether, diethylene glycol monomethyl ether, diethylene glycol monoethyl ether, polyethylene glycol, propylene oxide, propylene glycol, polypropylene glycol, 1, 3-butylene glycol, glycerin, pentaerythritol, sorbitol, mannitol and the like.

  Esters Isopropyl myristate, isopropyl palmitate, butyl stearate, hexyl laurate, myristyl myristate, oleyl oleate, decyl oleate, octyldodecyl myristate, hexyldecyl dimethyloctanoate, cetyl lactate, myristyl lactate, diethyl phthalate , Dibutyl phthalate, lanolin acetate, ethylene glycol monostearate, propylene glycol monostearate, propylene glycol dioleate, etc.

  Metal soaps Aluminum stearate, magnesium stearate, zinc stearate, calcium stearate, zinc palmitate, magnesium myristate, zinc laurate, zinc undecylenate, etc.

  Gum, saccharides and water-soluble polymer compounds Arabic gum, benzoin gum, dammar gum, guaiac gum, Irish moss, Karaya gum, tragacanth gum, carob gum, quinseed, agar, casein, lactose, fructose, sucrose and its esters, trehalose and its Derivatives, dextrin, gelatin, pectin, starch, carrageenan, carboxymethyl chitin or chitosan, hydroxyalkyl (C2-C4) chitin or chitosan with addition of alkylene (C2-C4) oxide such as ethylene oxide, low molecular chitin or chitosan, Chitosan salt, sulfated chitin or chitosan, phosphorylated chitin or chitosan, alginic acid and its salt, hyaluronic acid and its salt, chondroitin sulfate and its salt, heparin, ethylcellulose, methylcellulose, carbo Polymethyl cellulose, carboxyethyl cellulose, sodium carboxyethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, nitrocellulose, crystalline cellulose, polyvinyl alcohol, polyvinyl methyl ether, polyvinyl pyrrolidone, polyvinyl methacrylate, polyacrylate, polyethylene oxide, polypropylene oxide, etc. Alkylene oxide or its crosslinked polymer, carboxyvinyl polymer, polyethyleneimine, etc.

  Surfactant Anionic surfactant (carboxylate, sulfonate, sulfate ester, phosphate ester salt), cationic surfactant (amine salt, quaternary ammonium salt), amphoteric surfactant: carboxylic acid type amphoteric interface Activator (amino type, betaine type), sulfate type amphoteric surfactant, sulfonic acid type amphoteric surfactant, phosphate ester type amphoteric surfactant, nonionic surfactant (ether type nonionic surfactant, ether Ester type nonionic surfactant, ester type nonionic surfactant, block polymer type nonionic surfactant, nitrogen-containing type nonionic surfactant), other surfactants (natural surfactants, protein hydrolysates) Derivatives, polymer surfactants, surfactants containing titanium and silicon, fluorocarbon surfactants).

  Various vitamins Vitamin A group: retinol, retinal (vitamin A1), dehydroretinal (vitamin A2), carotene, lycopene (provitamin A), vitamin B group: thiamine hydrochloride, thiamine sulfate (vitamin B1), riboflavin (vitamin B2), pyridoxine (vitamin B6), cyanocobalamin (vitamin B12), folic acid, nicotinic acids, pantothenic acids, biotins, choline, inositols, vitamin C group: ascorbic acid and its derivatives, vitamin D group: ergocalciferol ( Vitamin D2), cholecalciferol (vitamin D3), dihydrotaxosterol, vitamin E group: tocopherol and its derivatives, ubiquinones, vitamin K group: phytonadione (vitamin K1), menaquinone (vitamin K2), menadione (bi) Min K3), such as menadiol (vitamin K4). Various amino acids valine, leucine, isoleucine, threonine, methionine, phenylalanine, tryptophan, lysine, glycine, alanine, asparagine, glutamine, serine, cysteine, cystine, tyrosine, proline, hydroxyproline, aspartic acid, glutamic acid, hydroxylysine, arginine, Ornithine, histidine, etc., and their amino acid derivatives such as sulfate, phosphate, nitrate, citrate, or pyrrolidone carboxylic acid.

  Various additives derived from plant or animal raw materials These are processed in a conventional manner according to the type and form of the product to be added (for example, grinding, milling, washing, hydrolysis, fermentation, purification, pressing, Extraction, fractionation, filtration, drying, pulverization, granulation, dissolution, sterilization, pH adjustment, deodorization, decolorization, etc. are arbitrarily selected and combined), and selected from various materials. That's fine. The solvent used for the extraction may be selected in consideration of the usage of the product to be provided (food, external use, bath use), processing to be performed later, etc. Usually, water, a water-soluble organic solvent (for example, It is desirable to use one or a mixture of two or more selected from ethanol, propylene glycol, 1,3-butylene glycol and the like. However, when it is not preferable to contain an organic solvent depending on the application, only water may be used, or ethanol that can be easily removed after extraction may be used alone or in any mixture with water.

  In addition, when applying additives derived from plant or animal-based materials to external preparations such as skin care and hair care and bath preparations, it not only protects skin and hair, but also moisturizes, improves touch and texture, imparts flexibility, and stimulates Relaxation, relaxation of stress due to fragrance, cell activation (cell aging prevention), suppression of inflammation, improvement of skin and hair quality, prevention of rough skin and its improvement, hair growth, hair growth, hair loss prevention, imparting gloss, cleansing effect, fatigue In addition to cosmetic effects such as relaxation of blood flow, blood flow promotion, and warm bath effect, it can be expected to have flavoring, thickening, antiseptic, and buffering.

  In edible products, nutritional supplementation, fatigue recovery, tonicity, cell activation (cell aging prevention) and other health and beauty effects, as well as improved taste, color tone, aroma, gloss, stabilization, thickening, It can be used for antiseptic purposes. In addition to this, we expect various cosmetic and pharmaceutical effects of each of the raw materials known so far, and by combining these, we aim to enhance the target effect, or are multifunctional It is also possible to make the product expected to be effective.

  Specific plants (herbal medicines) used as raw materials include, for example, Ai (blue leaves), Akazura (Seifu Fuji), Aotsuki-rafuji (Kisobuchi), Acacia, Akasuguri fruits, Red grapes, Akamegasiwa (red buds), Akane (Apricot roots) , Akajioji (chiyo), Agi (Atsu), Akinire (Chibara), Akebii (Kitsutsu), Asa (Asako Jin), Asagao (Toshiboshi), Ashitaba (Tomorrow), Azuki (Red Azuki), Asenyaku ( Asen Yaku), Acerola, Anise fruit, Abemaki fruit, Avocado, Ama, Achacha (Amacha), Amadokoro (Tamatake), Amigasayuri (shellfish mother), Almond, Altea, Arnica, Aloe (Amber), Aloe vera, Apricot (Anzujin) , Ansokkou (benzoic incense), Igakozolina (Gold head), Japanese knotweed (tiger cane root), Strawberry, Fig (no flower fruit) or its leaf, Ichibi (winter coconut), Japanese swordfish (distant), Carob, rice seed or seed coat, Inondo seed , Nettle, ylang-ylang, ginkgo (Ginkgo leaves, Ginkgo), Ikuki-sou-sou, fennel (scented incense), Ukiyagara (three-ridges), Uguisukagura fruit, turmeric (Usu-kin), usbeniaoi, usbenitachiaooi, usubasaicin (spicy), Nepenthes (Summer hay), Udo or Shishido (Clamming live, single lived, Tang Dynasty), Ume (Plum plum) or its flesh, Vulture, Uwaurushi, Eunshu mikan (Chen), Estragon, Ezoukogi (Chagoka), Enoki, Ebisugusa ), Elderberry fruit, Elemi, Enju (槐花, 槐花 米), Ogi (yellow 耆), Oulen (yellow ren), Great-crowned cucumber (caronin), Otsu ヅ rafuji (prevention), Psyllium (car front child, car front grass) , Toucan (palm), Ovana okera (white birch), Obayaya Shabushi fruit, Oha hawthorn (Yamakoko), barley (barley), oguruma (overturn), okra fruit Okera (white birch), Panax ginseng (carrot), Hypericum perennial (cutting younger brother), Odorikosou (continuous), Salamander (amber ear), Onigurumi, Oninoyara (Tempa), Onyuri (lily), Oninos, Halibut (Defeated soy sauce), Dutch strawberry, Dutch pepper, Dutch parsley, Dutch honey, olive, oregano, orange fruit or pericarp, silkworm (ripe yellow), seaweed [green algae, brown algae, red algae], cacao seeds, oysters or their leaves (柿蒂), Kagikazura (Katsufuji Rin), Cassia, casino berries (fruit), gadgets (莪 朮), kashiwa (bamboo tree, persimmon leaves), Cascarilla, crab (Kinsato), valerian (Valgaris radix), pumpkin , Kapokkinoki seeds, Kahoku salamander (蜀椒), Catfish (蒲 yellow), Chamomile, Camillar deer (Tongweed), Crow crow (Wang 瓜), Oats, Ravens (half-summer), Guarana seeds, Kalaya, Ca (Kiso), Kawayanagi, Kawaratake, Kawaramugi (Kin Chin), Kansui (Gansu), Kanzo (licorice), Candelilla, Kantou, Kanna, Strawberry, Kiwi fruit, Kikarasuuri (Gurone), Kyokyo Bellflower root), chrysanthemum (chrysanthemum flower), scallop (fruit berry), scallop (sheep), kizuta, yellow aloe vera, yellowfin mint, linden, yellowfin (yellow japonicus), yellow butterfly (yellow japonicus), gymnema silvesta, kiraja saponaria, kiraya, Cabbage, cabbage immature fruit, cucumber, kumquat fruit, betel, goldfish (Sengakukakusa), guayule, quercus infectria (garlic), kukui nut, wolfberry (bamboo shoots, coconut leaves, bamboo leaves, ground bark), cypress casserole (Tenmon winter) ), Kudzu (Kazunori), Kusunoki, Gooseberry fruit, Gardenia (Yamashiko), Kunugi (Yamasou), Kumazasa, Kumatsutsura (horse whip grass), Kura La (Bitter), Cranberry fruit, Chestnut or its astringent skin, Curkurigo / Latifolia fruit, Grapefruit fruit, Black-bellied red, Black-headed beetle (emergency), Black-bellied kokoshi (Extended life grass), Chlorella, Mulberry (mulberry leaf, Mulberry white skin), Guwaba Fruits, Keigai (bamboo shoots, pine ears), Celosia (chicken flowers, hens), Keirinsaishin (spicy), laurels, gentians, Gennoshiko (old lady), cilantro fruits, Koshinbara (monthly flowers), Kouseihakka, Kouzo fruit, black tea, Kohone (river bone), Kouhon (Etsumoto, Karamotomoto), Koryang, Kokemomo (Etsu Tachibana), Koganebana (Yellow Gon), Kogomebaotogiriso, Coconut fruit, Goshuyu (Kurei), Gosho Strawberry , Pepper (pepper), copaiba balsam, coffee beans, kobushi (hot pepper), burdock (beef bowl, beef coconut), kobotan, sesame (sesame), sesame seeds (Ginseng), wheat (wheat), rice or rice bran (red cocoon, white birch), cola acminata seeds, cola vera seeds, fenugreek fruit, colombo, condurango, kombu, comfrey (birch grass), konjac, saxima Button zuru (weiken sen), cherry blossoms, leaves, fruits, bark (cherry bark), pomegranate, sasa, lily (lily), sasanqua, sardine sardine (sawai), sweet potato, sugar cane, sugar beet, honey beet Saffron (Bansai flower, West red flower), Pomelo fruit, Savonsou, Salmon berry fruit, Salamander (Sesame), Salvia, Sagaki (mountain curd), Sawagurumi leaves, Sankirai (Yamagi), Hawthorn 37 ginseng), Sanshuyu (Yamamuro), Sansho (Yamamuro), Sandskon (Yamazune), Cyano fruit, Shiitake mushroom, Diou (jiki), Zion (purple grape), Digitally , Sikunshi (Ashi Kimiko), Shishido (Self life, Tang Dynasty), Perilla, Ajiso, Chiri-mendiso, Catamendizo (Shiyoha, Shisoko), Weeping birch (White birch), Rosewood, Shinanoki, Shiraren-gyo (ream), Shikakaniku (Chrysanthemum) , Shimotsetsu, Potato, Peonies (Shakuyaku), Shajin (Shasan), Janohige (Bakumon Winter), Shirakashi Seeds, Shirogoricho Seeds, Shirobanairis, Shirobanatsuta Flowers, Shirominanten (Southern Fruits), Juzudama, Palm Fruit, Ginger (Ginger), Shobu (Mushroom, Sone), Shozuk Fruit, Birch, Cinchona Sylbula, Cinnamon, Watermelon (Sairan), Honeysuckle (Gin Silver Flower, Shintofu), Sorrel (Acid), Swimberry Fruit, Strawberry Fruit, Sugina (Question), stevia, plum fruit, pine cones, larch, ivy, guru Mi, pear fruit, psyllium serrata, psyllium serrata, psyllium serrata, elderberry, psyllium serrata, mint, scallop, mistletoe, scorpiona, horseradish, pebbles, mallow, sedge, seri Senkyu (river cucumber), senna fruit or leaf, senninsou (daikon), senburi (this medicine), soka (grass), buckwheat seeds, rice potato, daiou (daihuang), radish, soybean, daidai (orange peel, fruit) , Thyme, Takatodai (Daikan), Takara-bi (Dark spine), Dark sweet cherry fruit, Tachiko Sou (Hundred incense), Tachibana (Tachibana bark), Tachiyanagi, Tamarind seed, Onion, Tamshiba (barb), Taranoki or its root bark, Tanjin, Danmaru, Cherry fruit, Chigaya or its root ), Chicory, clove (clove, clove), ginseng (gomi), ginseng (daihuang), ginseng (ginseng), ginseng (carrots), ginseng (ginger leaves), choreimaitake (ginger), Camellia, camellia, camellia, clover (lacquered weed), camellia (duck weed), tsurumazuki (red azuki bean), tsudukudami (what neck moth), tsuruna (apricot apricot), tun carrot (four leaves ginseng), camellia, deco, teuchigurumi, duberry fruit , Tengusa, Tendai Yaku (glaze), Toga (winter coconut), Pepper (Panyu), Toki (Toki), Red pepper, Corn or corn hair (Southern cocoon hair), Scotch mochi (Sadako), Torindou (Ryobi) ), Dokudami (10 medicines), Tokon (gum root), Tochiban ginseng (Takebana ginseng), Tochu (Tochu, Tochu), Tomato, Tragacanth, Triacanthus seed, Tolmen La, Dronoki, Toro-Aoi, Nigerian berry fruit, Chinese yam (Yakuyaku), Nagi Ikada, Naginata Koju, Nazuna, Natsume (Otsuka), Naniwaibara (Golden Cherry), Narco lily (Yellow spirit), Peanut (peanut), Nanten Minami Tenji), Nigaki (Nagi), Nigorigi (Anniversary), Nikuzuku, Nikkei (Kizuna), Nira (Otsuko), Elderberry (bone graft), Carrot, Garlic (Otsuchi), Nurde (Pyto), Leek, Noami (Otsuchi), Neubara (fruit), Yarrow, Nodake (Apricot), Novara, Peach, Palm, Hibiscus (Bussouge, Furinbussouge, Roselle), Pineapple fruit, Hakatayuri (Yuri), Hakamaraboshi (Bone fracture), Hakusen (White husk), hakuruberry fruit, chickweed (breeding), hazelnut (coconut), ashridokoro (roth root), basil, lotus (lotus meat, lotus), parsley, batata, ha Cucumber (bamboo moth), patchouli, mint (thin load, light load), pearl barley (yokuinin), hanasuge (wisdom mother), banana, blossom mint, vanilla bins, papaya, clover (bamboo grass), hub grass, paprika, clam (vine) Saeko, Hamasuge (Katsukiko), Habibishi (Shitsuko), Hamanasu (My cocoon flower), Hamaboufu (Hamaboshi), Hamamerisu, Rose (Rose), Parietalia, Harunire (Chiberm, 楡 白皮, 楡 葉), Pannoki, Hinogi (dried), Higangeturinjin (participant), pecan nuts, ganbanana (stone wall, vine pearl shahua), hikikoshi (long life grass), Japanese cypress (hisami tree), pistachio, beet, human camellia (stone wall), Hinata Inokozuchi (cow knee), hiba, castor, sunflower, bell pepper, himegama (incense), Japanese apricot, pimenta fruit, ayu fruit, yellow-bellied oyster, white-bellied oyster (bald eagle), hirohasenega (senega), loquat Bamboo leaf), betel nut (big belly skin, coconut), Japanese quail (Nanto), Japanese burdock (Fuyu winter flower), Japanese dandelion (Fuyu winter flower, Futaba winter leaf), Fujibakama (Orchid), Fuji bean (Tsuname), Butcher bloom, Grape peel Or fruit, beech, Fuyumushinatsusatake (Cycium Cordyceps), Brazilian licorice, black currant fruit, blackberry fruit, plum fruit, flucellaria, blueberry fruit, prunes, blond psyllium, bundo (mung bean), loofah, safflower (safflower), Belladonna, Berry fruit, Boysenberry fruit, Bow-Fu (windproof), Spinach, Japanese physalis (Torone), Honoki (Park, Japanese-Park), Bokeh (Kiso), Hosobaokera (Kaki), Hosobabareniku, Bodaiju, Button (peony skin) ), Hops, jojoba, white berry fruit, honan (apricot kernel), hon shimeji, mizurutennansho (Tennan) Star), maou (mao), macadamia nut, macri (marine grass), mugwa (mulberry leaf, mulberry white skin), mushroom (bamboo moth), matatabi (wood tengu), matsukasa, matsuhod (maritime), marigold, Malvanojadine, Mulberry fruit, Quince, Maronie, Mango, Mangosteen, Manshugurumi, Mandarin fruit, Mannen bamboo (Ganoderma lucidum), Mannenrou, Citrus genus fruit (fruit berry), Mishimashiko (Shibahu), Mizokakushi (Hananen lotus) ), Misohagi (Chikuma), Mitsugashi, Mitsuba, Mitsubahamago (Vulgaris), Green mint, Mimosa, Miogua, Miracle fruit fruit, Myrrh, Milobaran, Mugge (Kiso), Mukunoki, Mukuroji, Murasaki (purple root), Murasaki Maize, swordfish (benus), burdock, melaroica, melissa, merirot, melon fruit, mugwort, mosochiku, mo Leng (powder), mokko (wood incense), peach fruit, peach fruit, seed (peach seed) or leaf (peach leaf), coconut, morello cherry fruit, morohaya, yakawa murasimo, yakchi (masashi), cornflower, Cornflower, cornflower, yashabushi, yachiyagi, pine tree (octagonal gold plate), mistletoe (willow parasite), willow leaf, yabgarashi, yabukouji (purple gold beef), pokeweed (shoku land), yam yam (mountain medicine), yamahanoki (Yamamuro), Japanese yam (Plum skin), Eucalyptus, Yucca brevifolia, Yukinoshita (Tiger ear grass), Yuzu fruit, Lily, Mugwort (Leaf), Yoroigusa, Lime fruit, Peanut, Rakanka fruit, Raspberry fruit, Lavender, Longan (longan), green tea, apple fruit, lubus suabisimus (cool), lettuce, red currant fruit, lemon fruit, lemongrass, forsythia There are cormorants (regular), forsythia, wax palm, loganberry fruit, rosehip, rosemary, roman chamomile, wakame, wasabi, and bitumen.

Animal-derived raw materials include chicken crown extract, bovine / human placenta extract, porcine / cattle stomach, duodenum or intestinal extract or degradation products thereof, collagen derivatives such as water-soluble collagen, acylated collagen, etc. , Collagen hydrolyzate, elastin, elastin hydrolyzate, water-soluble elastin derivative, keratin and its degradation product, silk protein and its degradation product, porcine and bovine blood cell protein degradation product (globin peptide), porcine and bovine hemoglobin degradation product (Hemin, hematin, heme, protoheme, heme iron, etc.), casein degradation products, chicken eggs, fish meat and their degradation products.

  Microbial culture metabolite rice fermented extract, rice bran fermented extract, Euglena extract, lactic acid fermented milk powder, etc.

  α-hydroxy acid glycolic acid, citric acid, malic acid, tartaric acid, lactic acid and the like.

  Inorganic pigments Silicic anhydride, magnesium silicate, talc, kaolin, bentonite, mica, titanium mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, calcium carbonate, magnesium carbonate, yellow iron oxide, bengara, black Iron oxide, Gunjo, chromium oxide, chromium hydroxide, carbon black, calamine, etc.

  UV absorbers p-aminobenzoic acid derivatives, salicylic acid derivatives, anthranilic acid derivatives, coumarin derivatives, amino acid compounds, benzotriazole derivatives, tetrazole derivatives, imidazoline derivatives, pyrimidine derivatives, dioxane derivatives, camphor derivatives, furan derivatives, pyrone derivatives, Nucleic acid derivatives, allantoin derivatives, nicotinic acid derivatives, vitamin B6 derivatives, oxybenzone, benzophenone, guaiazulene, shikonin, baicalin, baicalein, berberine, etc.

Astringent lactic acid, tartaric acid, succinic acid, citric acid, allantoin, zinc chloride, zinc sulfate, zinc oxide, calamine, zinc p-phenolsulfonate, potassium aluminum sulfate, resorcin, ferric chloride, tannic acid and the like.

  Antioxidants ascorbic acid and its salts, stearic acid ester, tocopherol and its ester derivatives, nordihydrogua ceretenoic acid, butylhydroxytoluene (BHT), butylhydroxyanisole (BHA), parahydroxyanisole, propyl gallate, sesamol, Sesamoline, Gossypol, etc.

  Anti-inflammatory agent ictamol, indomethacin, kaolin, salicylic acid, sodium salicylate, methyl salicylate, acetylsalicylic acid, diphenhydramine hydrochloride, d or dl-camphor, hydrocortisone, guaiazulene, camazulene, chlorpheniramine maleate, glycyrrhizic acid and its salts, glycyrrhetinic acid and Its salt and so on.

  Disinfectant / disinfectant acrinol, sulfur, benzalkonium chloride, benzethonium chloride, methylrosaniline chloride, cresol, calcium gluconate, chlorhexidine gluconate, sulfamine, mercurochrome, lactoferrin or hydrolysates thereof.

Perfumes Natural perfumes such as musk, civet, castorium, ambergris, anise essential oil, angelica essential oil, ylang ylang essential oil, iris essential oil, fennel essential oil, orange essential oil, cananga essential oil, caraway essential oil, cardamom essential oil, guayakwood essential oil, cumin essential oil, Black letter essential oil, cinnamon essential oil, cinnamon essential oil, geranium essential oil, copaiba balsam essential oil, coriandel essential oil, perilla essential oil, cedarwood essential oil, citronella essential oil, jasmine essential oil, gingergrass essential oil, cedar essential oil, spearmint essential oil, western peppermint essential oil , Tuberose essential oil, clove essential oil, orange flower essential oil, winter green essential oil, trout balsum essential oil, buttery essential oil, rose essential oil, palmarosa essential oil, cocoon essential oil, hiba essential oil, sandalwood essential oil, petit gren essential oil, bay essential oil, vetiva essential oil, bergamot essential oil Peruvian balsam essential oil, boad rose essential oil, melamine essential oil, mandarin essential oil, eucalyptus essential oil, lime essential oil, lavender essential oil, linaloe essential oil, lemongrass essential oil, lemon essential oil, rosemary essential oil, Japanese mint essential oil, and other synthetic flavors Such.

  Dye / Colorant Red Cabbage Dye, Red Rice Dye, Akane Dye, Anato Dye, Ikumi Dye, Turmeric Dye, Enju Dye, Krill Dye, Agate Dye, Caramel, Gold, Silver, Gardenia Dye, Corn Dye, Onion Dye, Tamarind Dye , Spirulina pigment, buckwheat whole plant pigment, cherry pigment, laver pigment, hibiscus pigment, grape juice pigment, marigold pigment, purple potato pigment, purple yam pigment, lac pigment, rutin, etc.

  Sweetener sugar, sweet tea, fructose, arabinose, galactose, xylose, stevia, mannose, erythritol, maltose, honey, glucose, miraculin, monelin, licorice extract, etc.

  (Nutrition enhancer shell calcined calcium, cyanocolabamine, yeast, wheat germ, egg yolk powder, hemicellulose, etc.)

Other moisturizers, hormones, sequestering agents, pH adjusters, chelating agents, antiseptic and antibacterial agents, cooling agents, stabilizers, emulsifiers, animal and vegetable proteins and their degradation products, animal and vegetable polysaccharides And its degradation products, animal and plant glycoproteins and their degradation products, blood flow promoters, anti-inflammatory agents, antiallergic agents, cell activators, keratolytic agents, wound treatment agents, foam enhancers, thickeners, oral use Additives, deodorants / deodorants, bitters, seasonings, enzymes, etc. can be raised, and in combination with these, additive and synergistic effects can be expected.

(Gramineae plant extract production method 1)
2 kg of dried Kumizasa leaves and straws crushed to 0.5 to 5 mm and charged in a high-pressure steam can were charged with 2 MPa of high-pressure steam generated by a once-through boiler with a dryness measurement system of 99. Gas-liquid separation was performed with a separator that was confirmed to be achieved up to 5%, and steam with improved dryness was further introduced into a high-pressure steam can by reducing the pressure to 1.6 MPa with a pressure reducing valve. At the beginning of steam introduction, the air vent valve is opened and the steam is allowed to pass through the steaming can for 10 minutes at 100 ° C. to expel air, the air vent valve is closed and the temperature is raised from 100 ° C. to 180 ° C. over 10 minutes. Then, the pressure was released at once after holding for 10 minutes (steaming conditions: temperature 180 ° C. for 10 minutes). Leave high pressure steamed leaves and straw in a pressure extractor, add 8 kg of water, raise the temperature from room temperature to 110 ° C. over 30 minutes, hold at that temperature for 5 minutes, and then release the pressurized state over 1 hour . The extract was filtered and concentrated under reduced pressure to obtain a Sasa extract having a heating residue of 50%.

(Gramineae plant extract production method 2)
400 g of dried corn peel with a water content of 7% pulverized to 0.5-5 mm and 400 g of pure water (water content 50% by weight) are placed in a high-pressure steam can and heated to 160 ° C. over 10 minutes, at the same temperature. After holding for 10 minutes, the pressure was released at once (cooking conditions: temperature 160 ° C. for 10 minutes). 4 kg of water was added to the leaves and straws that had been steamed under high pressure, the temperature was raised from room temperature to 110 ° C. over 5 minutes, and the pressure state was released after holding at that temperature for 30 minutes. The extract was filtered and concentrated under reduced pressure to obtain a corn peel extract having a heating residue of 50%.

(Gramineae plant extract production method 3)
400 g of dried rice leaves and stems crushed to 0.5 to 5 mm and having a moisture content of 7% and 400 g of pure water (water content 50% by weight) are charged into a high-pressure steam can and heated to 200 ° C. over 10 minutes. The pressure was released at a stretch after being held at a temperature of 10 minutes (steaming conditions: temperature 200 ° C. for 10 minutes). 4 kg of water in which 1.8 g of sodium hydroxide was dissolved was added to the leaves and straws that had been steamed under high pressure, and the temperature was raised from room temperature to 110 ° C. over 5 minutes. The extract was filtered and concentrated under reduced pressure to obtain a rice extract having a heating residue of 50%.

(Tumor suppression)
The Ehrlich tumor cell line was transplanted subcutaneously into the groin of 10 mice, and the day of tumor cell transplantation was defined as day 0. The Gramineae plant extract obtained by Production Method 1 was orally administered once / day from 1 week before tumor transplantation to 2 weeks after transplantation.
The tumor volume was measured on the 5th, 10th, and 14th days of transplantation, and the tumor weight was measured on the 14th day of transplantation, and the average values were compared. Tumor volume (mm ³ ) was calculated as major axis (mm) × minor axis (mm) 2 × 0.5.

[Example 2] (NK cell activation)
The spleen is removed from the mouse that has been observed in Example 1, and a cell suspension is prepared. This suspension is overlaid on the Ficoll-Conray solution and centrifuged to separate NK cells, which is used as an NK cell suspension (E). NK cell activity after culturing a suspension of K562 cells (human chronic myeloid leukemia cells) labeled with 51Cr (200μCi) and preparing E: T = 50: 1. Activity was measured.

[Example 3] (Lymphocyte activation)
A cell suspension was prepared by removing the spleen from the abdomen of 5 normal mice. After adjusting the number of cells, each test substance solution, PBS (-), LPS (10 μg / ml), Con A (10 μg / ml) and production are prepared in each well (use n = 3) of each cytokine measurement plate. Method 1 (10, 100, 300 ug / ml) was added in an amount of 20 μl, followed by culturing for 72 hours. IL-2, TNFα and IFNγ in the culture supernatant after centrifugation were measured by ELISA.

[Example 4] (Antioxidant effect)
In accordance with the method modified by T. Hatano et al. [Chem. Pharm. Bull. Vol. 37, (8) 2016-2021 (1989)], the antioxidant activity of 1.0 g of each sample was converted to diphenyl picryl hydratyl (DPPH). The titer for eliminating 0.05 μmol of radical, ie 19.716 μg, was measured as one unit.

[Example 5]
Manufacturability of various products was examined using the plant extract obtained in the present invention. In addition, the unit of the following numbers is weight%.
(1) Health food (tablets)
A product was made using a composition of the following formulation: Tablets with good appearance and good appearance were obtained.
1. Dry powder of Sasa extract of production method 1 10
2. Plant extract powder (Dokudami, carrot, perilla) 30
3. Royal jelly powder 5
4). Collagen powder 5
5. Lactose 25
6). Corn starch 20
7). Hydroxypropyl cellulose 4
8). Magnesium stearate 1

The product was manufactured according to the following formulation. There was no problem in the manufacturing process due to the addition of the plant extract.
(2) Beverages for health Dry powder of corn peel extract of production method 2 4
2. Honey 15
3. Citric acid 0.1
4). dl-malic acid 0.1
5. Plant extract (chlorella, cinnamon) 20
6). D-sorbitol solution (70%) 10
7). Sodium benzoate 0.05
8). Perfume appropriate amount 9. Residue with 100 purified water

(3) Fruit juice beverages 1. Glucose liquid sugar 33
2. Grapefruit juice 65
3. Dry powder 0.5 of rice extract of production method 3
4). Perfume appropriate amount 5. Sour seasoning

The product was manufactured according to the following formulation. There was no problem in the manufacturing process due to the addition of the plant extract, and a good product was obtained.
(4) Lotion 1. Sorbit 2
2.1,3-Butylene glycol 2
3. Polyethylene glycol 1000 1
4). Polyoxyethylene oleyl ether (25E.O.) 2
5. Ethanol 10
6). Dry powder of Sasa extract of production method 1 1
7). Plant extract (Aloe, Hamamelis) 3
8). pH adjuster appropriate amount 9. Preservative appropriate amount
Ten. Residue with 100 purified water

(5) Emulsion 1. Squalane 3
2. Vaseline 1
3. Stearyl alcohol 0.3
4). Sorbitan monostearate 1.5
5. Polyoxyethylene (20) sorbitan monooleate 3
6.1,3-Butylene glycol 5
7). Dry powder of corn peel extract of production method 2 0.5
8). Water-soluble collagen 1
9. Preservative appropriate amount
Ten. Residue with 100 purified water

(6) Cream 1. Squalane 20
2. Beeswax 5
3. Refined jojoba oil 5
4). Glycerol monostearate 2
5. Sorbitan monostearate 2
6). Polyoxyethylene (20) sorbitan monostearate 2
7). Glycerin 5
8). Dry powder of rice extract of production method 3 0.3
9. Cow placenta extract 2
Ten. Preservative appropriate amount
11． Residue with 100 purified water

(7) Body soap Potassium laurate 15
2. Potassium myristate 5
3. Propylene glycol 5
4). Dry powder of Sasa extract of production method 1 3
5. pH adjuster appropriate amount 6. Preservative appropriate amount 7. Residue with 100 purified water

(8) Shampoo 1. Lauryl sulfate triethanolamine 5
2. Polyoxyethylene lauryl ether sulfate Na 12
3.1,3-Butylene glycol 4
4). Lauric acid diethanolamide 2
5. Edetate disodium 0.1
6). Dry powder of corn peel extract of production method 2 2
7). Hydroxypropyl chitosan 4
8). Perfume, preservative appropriate amount 9. Residue with 100 purified water

(9) Rinse 1. Stearyltrimethylammonium chloride 2
2. Cetostearyl alcohol 2
3. Polyoxyethylene lanolin ether 3
4). Propylene glycol 5
5. Dry powder of rice extract of production method 3 2
6). pH adjuster appropriate amount 7. Preservative appropriate amount 8. Residue with 100 purified water

(10) Hair art 1. Ethanol 50
2. Ethyl oleate 1
3. Polyoxyethylene (40) hydrogenated castor oil 2
4). Plant extract (Prunus periwinkle, assembly, hamamelis) 10
5. Dry powder 0.5% of Sasa extract of production method 1
6). Residue with 100 purified water

(11) Bath agent (A type)
1. Sodium bicarbonate 56
2. Anhydrous sodium sulfate 30
3. Borax 2
4). Dry powder of Sasa extract of production method 1 10
5. Plant extract powder (camomile, toki) 2

(12) Bath agent (B type)
1. Refined jojoba oil 5
2. Poloxyethylene sorbitan monolaurate 20
3. Glycerol monostearate 5
4). Liquid paraffin 2
5. Lauric acid diethanolamide 3
6). Dry powder of corn peel extract of production method 2 10
7). Plant extract (peony, aloe vera, chimpi) 10
8). Residue with 100 purified water

  The physiologically active composition of the present invention is extremely safe and has an immunostimulatory action, an antimutagenic action, an antibacterial action, an antioxidant action, an anticancer action, an intestinal environment improving action, a neutral fat reducing action, etc. It has the effect of. Due to overwork, stress, aging-related physiological aging, unbalanced diet, environmental changes, etc., our body tends to lose its function of maintaining homeostasis or become unbalanced. By using as a food and drink or cosmetics, it can be expected to have an effect of improving and improving these functions, or adjusting the balance and improving the health of the body to prevent disease.

  Furthermore, cancer, infectious diseases, and autoimmune diseases by adopting daily-use product forms such as health-oriented foods and drinks, luxury goods, livestock and pet feeds, and cosmetics (including bath preparations) It can be expected to prevent, reduce or improve allergies. In addition, it can be blended into medical preparations such as oral medicines, external medicines, injections / infusions, suppositories, etc., and is effective in maintaining the health of humans, pet animals, etc. and improving the constitution.

  Specific examples of foods and drinks include processed fishery products such as kamaboko, chikuwa and hanpen, processed meat products such as sausage, ham and wiener, agricultural processed products such as tofu, fried chicken and konjac, western confectionery, Japanese confectionery, bread, cake, jelly, Sweets such as pudding, snacks, cookies, gum, candy, ramune, noodles such as raw noodles, Chinese noodles, buckwheat noodles, sauces, soy sauce, dressing, mayonnaise, sauce, honey, powdered candy, syrups, syrups, curry powder , Spices such as mustard powder, pepper powder, jam, marmalade, chocolate spread, pickles, soy sauce, sprinkles, processed vegetables and fruits such as canned and bottled vegetables, fruits, cheese, butter, yogurt and other dairy products Fruit juice, vegetable juice, whey drink, soft drink, health tea, medicinal liquor, etc. Fees, other tablets for health such as nutraceutical the (nutraceutical) purposes, beverages, food products such health-oriented granulate, etc., animal feed, pet food, etc. as a target.

  Examples of cosmetic compositions include lotions, emulsions, creams, ointments, shaving mousses, oils, packs, shampoos, rinses, treatments, hair tonics, hair conditioners, hair restorers, permanent liquids, hair dyes. And skin care and hair care formulations such as body soaps, or bath preparations (any form such as liquid, powder, granule, solid, etc. may be used).

  Apart from these, sanitary products, wet tissue paper and paper towels, cotton, scratches, cuts, burns, scratches, inflammation, eczema, pimples, acne, rough skin, etc. It can also be applied to preparations and gauze impregnated with them, masks, bandages, eye care agents, inhalants, mouth washes, gargles, dentifrices and the like.

Claims (4)

  A physiologically active composition comprising a plant extract obtained by subjecting a plant to steaming at 120 to 250 ° C. followed by solvent extraction.   The bioactive composition according to claim 1, wherein the plant is a gramineous plant.   A method for producing a physiologically active composition, wherein a plant is steamed at 120 to 250 ° C. and then extracted with a solvent.   The method for producing a physiologically active composition according to claim 3, wherein the plant is a gramineous plant.