JPH1036283A - Fibroblast proliferation promoting agent - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain the subject agent containing two kinds of extracts originating from vegetables as essential components, effective for synergistically increasing the fibroblast proliferation promoting action and preventing and ameliorating various diseases of skin and/or scalp and useful as a preparation for external use for skin, a bathing agent, etc. SOLUTION: This agent contains (A) a water-extract of chlorella and (B) an extract of Aloe vera as essential components. The component A is produced by extracting raw or dried chlorella with water or hot water and the component B is produced by squeezing raw or crushed Aloe vera (leaf or stalk) or extracting with a solvent. The agent can be used as it is or in the form compounded to a preparation for external use for skin, a bathing agent or a food or drink. The compounding amount of each essential component is >=0.001wt.%, preferably 0.1-60wt.%. The agent is effective for the prevention and treatment of lesion such as eczema, chapped skin and atopic dermatitis.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to the use of a chlorella water extract and an aloe vera extract in combination to have a synergistic effect on the promotion of fibroblast proliferation, to prevent rough skin and skin swelling and to improve the skin. The present invention relates to an agent for promoting fibroblast proliferation which is effective for the prevention of aging, and its application to various skin external preparations, bath preparations and foods and drinks in the field of drugs, quasi-drugs or cosmetics.

[0002] The field of application is generally applicable to various internal and external preparations (including preparations used for animals), and specifically, ampules, capsules, pills, tablets, and powders. 1) Pharmaceuticals, 2) Quasi-drugs, 3) Foods, 4) Topical or systemic skin cosmetics, 5) Scalp / hair Pharmaceutical and / or cosmetic preparations (eg, shampoos, rinses, treatments, permanent solutions, hair dyes,
Hairdressing agents, hair tonics, hair growth / hair restoration, etc.), 6) bath agents for use in bath water, 7) other liquid odor / odor control agents, sanitary articles, sanitary cotton, wet tissues, etc. .

In addition, it is used for various injuries such as skin and scalp, oral cavity, eyes, ears, nose, anus, and genital area for the purpose of promoting the growth of skin and dermis, preventing skin aging, roughening the skin and promoting wound healing. be able to.

[0004] Foods and drinks further include oral compositions (gum, candy, etc.), processed seafood products such as kamaboko and chikuwa, livestock products such as sausage and ham, western confectionery, Japanese confectionery, raw noodles, Chinese noodles, and yuden noodles , Buckwheat and other noodles, sauces, soy sauce, sauce, sugar, honey, powdered candy, water syrup, and other spices, curry powder, mustard powder, pepper powder and other spices, jam, marmalade, chocolate spread, pickles, soy sauce, Processed vegetables and fruits such as sprinkles and canned and bottled vegetables and fruits, dairy products such as cheese, butter, yogurt, miso soup, soup, fruit juice, vegetable juice, whey drinks, soft drinks, alcoholic beverages, etc. Use in general foods and drinks such as health foods.

[0005]

2. Description of the Related Art Individual aging or various diseases caused by the aging have a correlation with aging of all cells that can divide (reduction of division rate and cell function). As a result, a large number of cell activators have been searched for. Among them, one of the cell activators is cell growth factor, and various factors have already been confirmed, and the applicant's connective tissue hydrolyzate (Japanese Patent Application Laid-Open No. 62-84024), and water solubility derived from organs such as thoracic line and spleen Protein (Japanese Unexamined Patent Publication No. 63-188697,63
-188698), or bovine placenta extract (JP-A-03-141299) or an extract of bovine brain, bovine pituitary gland, and cockscomb (JP-A-01-175)
998), which are used for quasi-drugs and cosmetics.

[0006]

However, these are all derived from animal systems. As plant-based ones, wolfberry is a cell activator (such as a cell activator, which promotes cell engraftment rate and colony formation). Japanese Patent Application Laid-Open No. 02-117686), but at present, no plant-derived plant having cell growth ability has been obtained.

[0007] In view of these circumstances, the present inventors have made various plants or seaweeds useful as fibroblast proliferation promoters the subject of development, and as a result, in Japanese Patent Application No. 7-212665, chlorella water The extract was found to have a fibroblast growth-promoting effect, and then used in various combinations with other plants. In combination with the aloe vera extract, the fibroblast growth-promoting effect was synergistic. It is found that it shows an increasing relationship and is effective for prevention and improvement of various diseases of skin / scalp (for example, eczema, rough skin, skin swelling, etc.) and is highly safe external preparation for skin and bath, food and drink Has led to the completion of the present invention.

[0008]

[Means for Solving the Problems]

DETAILED DESCRIPTION OF THE INVENTION "Aloe vera" used in the present invention means "Aloe barbadensis Mill." (= Aloe Vera), a plant of the genus Aloe of the family Liliaceae.
L.) and its variants (Aloe Vera L. var. Chinensis (Haw.)
Berger), (Aloe saponaria Haw.) Obtained from leaves or stems, but other species of the same species, Aloe arborescens Mill., And Socotra aloe (Aloe p.
erryi Baker, Natal aloe (Aloe bainesii Th. Dy)
er), Aloe dichotohoma L., Aloe marlothii (Aloe marlothii Berger), Aloe rupestris Baker, Aloe spinosis sima (Aloe spinosissima Hort.), Aloe plicatilis Mill (Aloe plicatilis Mill) .), Aloe aperifolia (Aloe asperifolia A.Berger), Aloe capitata polypolicola (Aloe captata Bak.var.cipolinic)
ola H.Perr.), Aloe splenden
s Lavranos), Aloe humilis Mil
l.), Aloe mitriformis Mil
l.), Aloe variegata L.,
Cape Aloe (Aloe ferox Mill.), Aloe Africana (Aloe africana Mill.), Aloe Spicata (Aloe)
spicata Baker), (Aloe haworthiodes Baker), (Al
oe succotrina Lam.,) can also be used.

[0009] The term "chlorella" used in the present invention refers to green plant phylum (Chlorophyta), green algal net (Chlorophycea).
e), Chlorococcales, Oocystaceae, Chlorella, a single-celled green alga of 3-10 microns in diameter, for example, Chlorella vulgaris (C. Vulgaris),
Chlorella pyrenoidosa (C. Pyrenoidosa), chlorella ellipsoidea (C. Ellipsoidea) and the like can be used.

In addition, other seaweeds include, for example, green algae (genus Aonori: Usvaaonori, Sugiaonori, Hiraaonori, Bouaonori, Hosoedaoonori, Chlamydomonas: Chlamydomonas, Akayukimo, Dunaliella: Dunaliella, Chlorococcus, Chlorococcus; : Kwanomimo, Volvox: Owhide sunflower, Volvox, Palmera, Artemisia,
Spirogyra: Hizaori, Spirogyra, Tulsimidro, Hibimidro: Hibimidoro, Aosa: Anaoasa, Amiosa, Nagaosa, Kawanori: Kawanori,
Genus Fricciella, genus genus: medusa, asamidori ogusa, kawashiogusa, marimo, valonia: tamagobaronia, tamabaronia, magnum: tamatamamo, iwata: husaiwata, suricogita, heraiwirata, crocodile, crocus Sakibutimimil, Nagamil, Hiramil, and Casanori:
Casa nori, Juzumo
Species of the genus Mizudzumo, Genus genus, Colecaetus, Genus genus genus, Species of the genus Rosa: Rosa spp. Genus (Suizenjinori: Suizeninori, Aokko, Nostoc: Kawatake, Ishikurage, Hassai, Yuremo, Racenmo (Spirulina): Spirulina,
Genus trichodesmium (Aikassio), brown algae (genus Kombu: makombu, lithicum, hosomekonbu, mitsuishikomubu, seaweed genus: seaweed, hirome, blue seaweed,
Lessoniaceae: Macrocystis pilifera, Macrocystis integrifolia, Neocitys luetokeana, Hijiki: Hijiki, Hibamata: Hibamata, Piraella: Piraella, Shiomimidro: Nagamiushimidoro, Isogrape: Isogrape, Isogawara: Isogawara , Black genus: Gunsen kurogashira, Kashirazaki: Kashirazaki, Muchimo: Muchimo, Hiramuchimo,
Kebelligsa, Amidigusa: Amidigusa, Sakibiroamiji, Sanadagusa: Sanadagusa, Fukulinamiji, Commongusa: Commongusa, Yahuzugusa: Ezoyahazu, Yahuzugusa, Urabashihazu, Zygamichusa, Umiuchiwachina, Umiuchiwachina , Genus: Somenaceae, genus Nabarimo: genus Navalimo, genus Chasoumen: genus Motsucha, genus Matsumo: matsumo,
Pine spider genus: Pine spruce, Pleurotus genus: Pleurotus genus: Pleurotus elegans, Pseudopodia genus: Pseudopodia genus, Phytopodia genus: Phytopodiac, Ishimozuku: Pleurotus elegans, Kuromo genus: Chromo, genus Nisemozuku: Pleurotus genus:
Mozuku, Ishige: Ishige, Irolo, Ichimegasa: Ichimegasa, Keyari: Keyari, Umibos: Umibos, Urushigusa: Urushigusa, Keurusigusa, Tobacco, Kombomodo: Kombudoki, Hambamodoki:
Genus Habanomoki, Genus Habanori: Havanori, Genus Habanori: Habanori, Common Bukuro: Common Bukuro, Ezobukuro: Ezobukuro, Fukuronori: Fukuronori, Watamo, Chishimafukuronori Genus: Genus Genus, Kagomenorimura, Genus Kamuramemura ： Genus Shark, Genus Genus: Genus Genus, Genus Genus: Genus Genus, Genus Genus: Genus Lily, Genus Genus: Genus Genus, Genus Genus:
Turmo, Anatome: Aname, Siberium: Sujime, Misujikombu: Misjikombu, Atsubamisujikombu, Kombu: Gatsugarakomubu, Oysterfish, Onicombu, Goheikombu, Nagakombu, Pea, Ootomikombu, Torokomokuto: Torokontokuto , Genus genus: swordfish, swordfish, black squid, genus scorpionum: squirrel, squirrel genus: squirrelcomb, kuroshiome: kuroshiome, scorpion genus: feline sacion, alame genus: alame, Ainuokame: Ainuwakame, squid, squid Ezoishige, Yabamoku: Yabamoku, Rappermoku: Rappermoku, Jiromoku: Uganomok,
Joromok, Hyemok, Hondawara: Tamanashimoku,
Isomok, Nagashimamoku, Akamoku, Shidamoku, Hondawara, Nejimoku, Narasamo, Mametara, Tatsukuri, Yasumatamoku, Umitranoo, Oobamoku, Fushizushimoku, Hahakimoku, Togemoku, Jolemoku, Sawtooth moku,
Species of the genus Saw-mushroom, Sugimoku: Sugimoku, Ukimo: Ouukimo, Buruquimo: Burukimo, Kayamonoli: Kayamonori, etc., Red algae (Hyjirimen: Hijirimen, Tengusa): Maxa (Tengusa), Hirakusa, Oxa, Obaxa Catalpa, Yatabegusa: Yatabegusa, Yuikiri: Yuikiri, Shimanegusa: Shimantengusa, Tosakanori: Tosakanori, Kirinsai: Togekirinsai, Akumasakirinsai, Kirinsai (Ryukyusunomata), Beetlefish
(Yahazunomata), Ezotunomata, Togetsunomata, Hiragotoji, Suginori: Genus Kotojinomata, Suginori, Shikinori, Kainori, Ibotsunomata, Hyusbanori:
Yaleusbanori, Kagiusbanori, Sujiusbanori, Hyusbanori, Ushikenori Genus: Ushikenori, Funorinoushike, Amanori Genus: Asakusanori, Susabinori, Upupinori, Oniamanori, Tasa, Filitasa, Benitasa, Rhodocornoni, Rhodocortonum Genus
Akebonomozuku, Konahada genus: Haikonahada, Yogorekonahada, Aokonahada, Sea elephant genus: Sea elephant, Tsukumonori, Kamogashiranori, Benimozuku genus: Benimozuku, Hosobenimozuku, Kasamatsu genus: Genus L., Genus L. : Rattles, Hirataragara, Hirohatamaidaki Genus: Hirohatamaidaki, Tamaitadaki Genus: Tamaitadaki, Kagikenori: Kaginori, Kagikenori, Tengusa: Himetengusa, Haitengusa, Oobusa, Nambugusa, Kohiran, Ohibora, Kohira, Ohibora
Genus genus: genus isensis, genus genus:
Genus spruce, Ryumonsou: Ryumonsou, Hera Ryumon, Nisekarequigusa: Nisekarequigusa, Okitsubara genus: Obaokitsubara, Akaba: Akaba, Malvaacaba, Naminohana: Genus Hosobanamino, Naminohanagosa, Coralthus Genus Ikawanokawa, Genus Kanokawa: Kainokawa, Genus Kaninote: Kaninote, Coral Genus: Coralum, Genus Mucadenori: Mucadenori, Suzukade, Catanori, Hiramukade, Kyonohimo, Sakuranori, Nikunukade, Tambanori, Tsurutsuru,
Genus Isonohana: Nuracusa, Chronulaxa, Stinging genus, Hirakintoki: Hirakintoki, Matabou: Matabou, Kintoki: Chabokintoki, Kintoki, Matsunori, Komenori, Tosakamatsu, Aedes pine, Kakraitus: Genus Odonata, Genus L. Nagaobane, Funori: Hanafunori, Fukuronori, Makuhnori, Kalexa: Kalexa, Tosakamaki: Hosobanotokamaki, Kurosakamaki,
Genus genus: genus genus: genus: kinuhada, genus: scorpion genus: ezotsaka, genus tsukasanori: enashikarimenia, otsukasanori, hanagatakarimenia, hohono: genus: lyca, genus genus: genus, genus, genus, genus, genus : Scarabaeni, Okamuragusa: Yamadagusa, Mirin: Mirin, Hosobamilin,
Tosakanori genus: Kikutosaka, Ezonameshi: Ezonameshi, Isomokka: Isomokka, Yukari: Yukari, Hosoyukari, Ibaranori: Ibaranori, Saidaibara,
Tachiibara, Kagiibaranori, genus
Genus Isodanthus: Genus Isanthus, Genus Atsubanori: Genus Atsubanori, Genus Ogonori: Ogonori, Tsurushiramo, Shiramo, Ogogonori, Mizokonori, Kabanori, Tengosamodoki Genus: Hachijotensamodenki, Fusikurenorikai, Genus genus: Genus: foxglove, saimi
Lotus spider, genus genus: Ikanoashi, Hosoibonori,
Nobonori, Black Snapper genus: Red Snapper, Red Snapper genus: Red Snapper, Hishbukuro genus: Hishbukuro, Madaragusa genus: Togemadara, Echinamadara, Taoyasou genus: Snapdragon scutellida , Hirata goat: Hirata goat, Darus: Dars, Masagoshibari, Anadals, Uebagsa: Uebagsa, Benifukuronori: Benifukuronori, Fushinagi: Fushitsunagi, Himefushinagi,
Species of the genus Hilohafushi and Snapdragon: Species of the Species of Hiragiwasoni, Species of the Species of Species: Species of the genus Igis, Keigis, Harigrees, genus Haneigis, Amixa and Egonori:
Egonori, Futogigis, Saeda Genus: Saeda, Chrysanthemum Genus: Chrysanthemum, Konohanori Family: Habutaenori, Konohanori, Suzushironori, Usveni Genus: Usbeni, Hasuginu Genus: Hasuginu, Nagakonohanori Genus: Naganosugino, Genus Genus Yaleusbanori, Sujiusbanori, Hyusbanori, Usubanorimodoki Genus: Usubanorimodoki, Ayanishiki: Ayanishiki, Ayaginu:
Ayaginu, Dajia: Enashidazia, Shimadagia: Isohagi, Shimadada, Dazimodoki: Dazimomodoki, Itogusa: Moleutogusa, Futoigusa, Makuri: Makri, Yanaginori: Hanayanagi, Yuna, Yanaginori, Motsuyuna, Sasa, Nayasa : Kurosozo, Kobusozo, Hanezozo, Sozonohana, Hanegusa: Hanegusa, Kanegusa, Kozanemo: Kozanemo, Isomurasaki, Hosokozanemo, Himegoke: Himegoke, Kurohimegoke, Hiodoshig, Genus: Betula spp., Pleurotus spp .: Pleurotus spp., P. spp .: P. spp., Sawworts: Pleurotus spp. Wamozuku, Himekawamozuku,
Ideyugogome spp .: Ideyugogome spp., Okitimozuku spp .: Okuchimozuku, Itogusa spp., Chinorimo spp .: Chinorimo, Tisginori spp .: Chisinori, etc .;
In the same manner, the fibroblast proliferation-promoting effect can also be expected in the genus Shiratama, the genus Hoshiturimo, the genus Hoshiturimo, the genus Lichnotumnus, the genus Hirasimo, such as the genus Himejimo, the Chabosugaramo, and the genus Tripera.

[0011]

BEST MODE FOR CARRYING OUT THE INVENTION The aqueous extract of chlorella used in the present invention is obtained by drying a chlorella raw material as it is or by drying it.
Extracted with water or hot water, it may be adjusted by optionally drying, concentrating, or diluting depending on the type and form of the fibroblast proliferation promoter, skin external preparation and bath preparation, food and drink to be applied.

The extract of aloe vera used in the present invention is the aloe vera (leaf, stem) raw material as it is, after crushing or extracting. Alternatively, aloe vera (leaf, stem) raw material is extracted as it is or after pulverization with a solvent. As the extraction solvent, any one selected from water, ethanol, anhydrous ethanol, 1,3-butylene glycol, and propylene glycol, or any combination of two or more thereof can be used. It can be used in a state where ethanol, anhydrous ethanol, 1,3-butylene glycol and propylene glycol extraction are combined. or,
The extract thus obtained may be optionally dried, concentrated, or diluted depending on the form and form of the fibroblast proliferation promoter to be applied, the skin external preparation and bath preparation, and the food or drink, and may be adjusted.

Although the production method is not particularly limited, it is usually sufficient to be within the range of the boiling point of the solvent at normal temperature to normal pressure. After the extraction, filtration or use of an ion-exchange resin is carried out. Purification may be performed to obtain a solution, paste, gel, or powder. In many cases, it can be used as it is, but if necessary, further purification treatment such as deodorization and decolorization may be added as long as the effect is not affected.
An activated carbon column or the like may be used as a purification treatment means for decolorization or the like, and any ordinary means generally applied to an extracted substance may be selected.

The essential components (aqueous chlorella extract and aloe vera extract) of the present invention can be used as a fibroblast proliferation promoter as they are, and can be further incorporated into skin external preparations, bath preparations, and foods and drinks. Although it is not particularly limited, it slightly varies depending on the type and quality of fibroblast proliferation promoter, skin external preparation and bath preparation, food and drink, and expected effect, but each of the essential components is usually 0. .
001% by weight or more (hereinafter, expressed as% by weight), preferably 0.1 to 60%. If the amount is less than 0.001%, the effect cannot be expected sufficiently.

The fibroblast proliferation promoter, skin external preparation, bath preparation, food and drink of the present invention may contain, in addition to the above-mentioned essential components, pharmaceuticals, if necessary, as long as the effects of the present invention are not impaired. It can be produced by arbitrarily selecting and using components and additives used in pharmaceuticals such as quasi-drugs, cosmetics, foods and beverages, and the like.

(1) Various fats and oils Avocado oil, almond oil, fennel oil, perilla oil,
Olive oil, orange oil, orange luffer oil, sesame oil,
Cocoa butter, chamomile oil, carrot oil, cucumber oil, tallow, tallow fatty acid, kukuinut oil, safflower oil, shea butter, soybean oil, camellia oil, corn oil, rapeseed oil, persic oil, castor oil, cottonseed oil, peanut oil, Turtle oil, mink oil, egg yolk oil, cocoa butter, palm oil, palm kernel oil, mocro, coconut oil, tallow, lard, or hydrogenated products of these oils (hardened oil, etc.).

(2) Waxes Beeswax, carnauba wax, whale wax, lanolin, liquid lanolin, reduced lanolin, hard lanolin, candelilla wax,
Montan wax, shellac wax, etc.

(3) Mineral oil Liquid paraffin, vaseline, paraffin, ozokeride, ceresin, microcrystalline wax, squalene, squalane, pristane and the like.

(4) Fatty acids Natural fatty acids such as lauric acid, myristic acid, palmitic acid, stearic acid, behenic acid, oleic acid, 12-hydroxystearic acid, undecylenic acid, tall oil, lanolin fatty acid, isononanoic acid, caproic acid , 2-ethylbutanoic acid, isopentanoic acid, 2-methylpentanoic acid, 2
-Synthetic fatty acids such as ethylhexanoic acid and isopentanoic acid.

(5) Alcohols Ethanol, isopiranol, lauryl alcohol,
Natural alcohols such as cetanol, stearyl alcohol, oleyl alcohol, lanolin alcohol, cholesterol, and phytosterol; and synthetic alcohols such as 2-hexyldecanol, isostearyl alcohol, and 2-octyldodecanol.

(6) Polyhydric alcohols Ethylene oxide, ethylene glycol, diethylene glycol, triethylene glycol, ethylene glycol monoethyl ether, ethylene glycol monobutyl ether, diethylene glycol monomethyl ether, diethylene glycol monoethyl ether, polyethylene glycol, propylene oxide, propylene glycol , Polypropylene glycol, 1,3-butylene glycol, glycerin, pentaerythritol, sorbitol, mannitol and the like.

(7) Esters Isopropyl myristate, isopropyl palmitate, butyl stearate, hexyl laurate, myristyl myristate, oleyl oleate, decyl oleate, octyl dodecyl myristate, hexyl decyl dimethyloctanoate, cetyl lactate, Myristyl lactate, diethyl phthalate, dibutyl phthalate, lanolin acetate, ethylene glycol monostearate, propylene glycol monostearate, propylene glycol dioleate, and the like.

(8) Metal soaps Aluminum stearate, magnesium stearate, zinc stearate, calcium stearate, zinc palmitate, magnesium myristate, zinc laurate, zinc undecylenate and the like.

(9) Gum, saccharides and water-soluble polymer compounds Arabic gum, benzoin gum, dammar gum, guaiac oil, Irish moss, karaya gum, tragacanth gum,
Carob gum, quinseed, agar, casein, lactose,
Fructose, sucrose and its esters, trehalose and its derivatives, dextrin, gelatin, pectin, starch, carrageenan, carboxymethyl chitin or hydroxyalkyl (C ₂ -C ₄ ) oxide to which alkylene (C ₂ -C ₄ ) oxide such as ethylene oxide is added _{2 to} C ₄ ) chitin or chitosan, low molecular weight chitin or chitosan, chitosan salt, sulfated chitin or chitosan, phosphorylated chitin or chitosan, alginic acid and its salt, hyaluronic acid and its salt, chondroitin sulfate and its salt, heparin, ethyl cellulose , Methylcellulose, carboxymethylcellulose, carboxyethylcellulose, sodium carboxyethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose, nitrocellulose, crystalline cellulose Polyvinyl alcohol, polyvinyl methyl ether, polyvinyl pyrrolidone, polyvinyl methacrylate, polyacrylic acid salts, polyalkylene oxide or crosslinked polymer thereof such as polyethylene oxide or polypropylene oxide, carboxyvinyl polymers, such as polyethyleneimine.

(10) Surfactant Anionic surfactant (alkyl carboxylate, alkyl sulfonate, alkyl sulfate, alkyl phosphate), cationic surfactant (alkyl amine salt, alkyl quaternary ammonium salt) ), Amphoteric surfactant:
Carboxylic acid type amphoteric surfactant (amino type, betaine type), sulfate ester type amphoteric surfactant, sulfonic acid type amphoteric surfactant, phosphate type amphoteric surfactant, nonionic surfactant (ether type nonionic) Surfactants, ether ester type nonionic surfactants, ester type nonionic surfactants, block polymer type nonionic surfactants, nitrogen-containing nonionic surfactants), other surfactants (natural surfactants) , Derivatives of protein hydrolysates,
Polymer surfactant, surfactant containing titanium and silicon,
Fluorocarbon surfactants).

(11) Various vitamins Vitamin A group: retinol, retinal (vitamin
A₁), Dehydroretinal (vitamin A_Two), Carochi
N, lycopene (provitamin A), vitamin B group: thia
Min hydrochloride, thiamine sulfate (vitamin B₁), Lvov
Rabin (vitamin B_Two ), Pyridoxine (vitamin
B₆), Cyanocobalamin (vitamin B₁₂), Folic acid,
Nicotinic acids, pantothenic acids, biotins, choline,
Inositols, vitamin C group: ascorbic acid and its
Derivatives, vitamin D group: Ergocalciferol (bi
Tamin D_Two), Cholecalciferol (vitamin D_Three),
Dihydrotaxerol, Vitamin E group: Tocophero
And its derivatives, ubiquinones, vitamin K group:
Tonadione (vitamin K₁), Menaquinone (vitamin
K_Two), Menadione (vitamin K_Three), Menadiol (bi
Tamin K_Four), Other essential fatty acids (vitamin F), mosquitoes
Lunitine, ferulic acid, γ-oryzanol, orot
Acids, vitamin Ps (rutin, eriocitrin, hesperi
Gin), vitamin U, etc.

(12) Various amino acids valine, leucine, isoleucine, threonine, methionine, phenylalanine, tryptophan, lysine, glycine, alanine, asparagine, glutamine, serine, cysteine, cystine, tyrosine, proline, hydroxyproline, aspartic acid, glutamic acid , Hydroxylysine, arginine, ornithine, histidine, and the like, and their sulfates, phosphates, nitrates, citrates, and amino acid derivatives such as pyrrolidone carboxylic acid.

(13) Various additives derived from plant or animal raw materials These can be processed in a conventional manner according to the type and form of the product to be added (for example, pulverization, milling, washing, hydrolysis, Fermentation, purification, squeezing, extraction, fractionation, filtration, drying, pulverization, granulation, dissolution, sterilization, pH adjustment, deodorization, decolorization, etc. You can choose to serve.

The solvent used for the extraction may be selected in consideration of the purpose and type of the product to be provided, or the processing to be performed later. However, usually, water and a water-soluble organic solvent (eg, ethanol) ,Propylene glycol,
1, selected from 1,3-butylene glycol)
It is desirable to use a species or a mixture of two or more species. However, when the content of the organic solvent is not preferable depending on the application, only water may be used, or ethanol which is easily removed after extraction may be used, and may be used alone or in an arbitrary mixed solution with water, Exploited extract may be used.

In addition, additives derived from plant or animal raw materials are
When applied to systemic or topical external preparations and cosmetics, including protection of skin and hair, moisturizing, improvement of feel and texture,
Gives flexibility, relieves irritation, relieves stress due to aroma, activates cells (prevents cell aging), suppresses inflammation, improves skin and hair quality, prevents and improves rough skin, hair growth, hair growth, hair loss prevention, gloss Application, cleaning effect, alleviation of fatigue, promotion of blood flow,
Other than cosmetic effects such as warm bath effect, fragrance, deodorization, thickening,
Antiseptic and buffering effects can also be expected.

In edible products, health and cosmetic effects such as nutritional supplementation, recovery from fatigue, tonicity, cell activation (prevention of cell aging) and the like, improvement of taste, provision of color tone, aroma, gloss, stabilization, etc. It can be used for thickening and preservation. Furthermore, in addition to this, various cosmetic and pharmaceutical effects of each known raw material are expected, and by combining them, the intended effect is enhanced or multi-functional. It is also possible to make a product that expects an effect.

Specific plants (crude drugs) as raw materials include:
For example, almond (Hen peach), eye (Aiba), blue oak
(Seifu Fuji), Aotsuji Lafzi (Kibo), Acacia, Akasuri Fruit, Red Grape, Red Megawiwa (Red Bud), Akane
(Kanekane Akane), Ayajiou Jiou (Jihuang), Agi (Awei),
Akinire (Royu skin), Akebi (Kidori), Asa (John Asako), Maruba Asagao or Asagao (Toshiko), Ashitaba (Asuka),
Azuki (red azuki), acerola, ase yak (Asenyaku), anise, Abemaki fruit, avocado, ama, amacha (sweet tea),
Amachaworu, Amadokoro (Jade bamboo), Amigasa lily (Shellfish), Altea, Arnica, Aloe (Length), Angelica, Anzu Honganzu (Anjin), Ansokkou (Azuka), Igakouzolina (Cholezoa), Knotweed (Tiger stick) , Strawberry, fig (apricot) or its leaves, strawberry (Aoiko),
Ichiyakuso, Ginkgo (Ginkgo biloba, Ginkgo), Itohimemegi
(Enshi), locust bean, rice seed or seed coat, Inondo seed, Ibuki-kusou-so, nettle, ylang-ylang, fennel (Fenka), ukiyagara (Mitsuryo), fragrant fruit, Himeuikyo, turmeric (Utsukushi),
Keirinsaishin (spicy), Usbania oyster, Usbenitachioi, Utsubogusa (summer hay), udo or shishido (Qang activity, independent activity, Tang dynasty activity), ume (Umebume) or its pulp, Vladigashi, Uwaurushi, Unshumikan (skin), estragon , Ezocogi (Ezo Goka), Enoki, Ebisugusa
(Keiko), Elderberry fruit, Elemi, Enju (Gossip flower, Sophora ginseng rice), Ogi, Kibana-ohgi (Ohgi), Ouren
(Yellow), giant crow squirrel (caronine), giant leopard
(Self-defense), psyllium (carnage, carnival grass), toucan (Haruko), Obananaokera okera (white jujube), Obanayashabushi fruit, O hawthorn hawthorn (Yamako), barley
(Barley), Oguruma (turning over), Panax ginseng and radish (carrot), Okra fruit, Hypericum persicae (Kotomebayotogirisou) (cutting grass), Odorikosou (intermittent), Onamimomi (blue ears), Onigurumi, Oninoyagara (Tianma), Oniyuri
Sasayuri, Hakatayuri (Yuri), Ononis, Halibut (Hihayu), Ominaeshi (Loose), Dutch strawberry, Dutch mustard (Cresson), Dutch jelly, Dutch honeybee, olive, oregano, orange fruit or pericarp, Kaikaeio Yellow), cacao seeds, oysters or their leaves (persimmons), kagikazura (chou tsuto), cassia, cassia fruit (mushrooms), gajutsu (shadowatsu), kasiwa (shadow tree), cascarilla, kanikusa (kinsha) Wisteria), valerian root (valerian root), birch or weeping birch (white birch), squash, capoknoki seed, kahokusansho (pepper), gama (bamboo), chamomile roman chamomile, chamiyatsude (tsukusa), karasuri (flower meadow), Karasubishaku (midsummer), oats, guarana seeds, karaya, karin
(Gourd), Kawayanagi, Kawatake mushroom, Kawamuragi (Inchinko), Kansui (Sweet), Kanzo (licorice), Candelilla, Kantou, Canna, Raspberry, Kiwi fruit, Kikarasuuri (Ururone), Kikyo (Kikyo, Bellflower) Root), chrysanthemum (chrysanthemum), catalpa (Azumi), sagebrush (yotei), pheasant (kotomi), kizuta, kidachi aloe, kidachi mint, chinoki, kinada (huangkashiwa), gimnema sylvester, cabbage,
Cabbage immature fruit, cucumber, Kiraja saponaria, Kiraya, kumquat fruit, betel, kinmizukiki (senkaku grass),
Guava fruit, guayule, Quercus infectria (gallicia), cucumber nuts, wolfberry (Gugi, gugi, gugi leaf, skeletal bark), cedar spruce (Tenmen winter), kuzu (kuzukane),
Camphor tree, gooseberry fruit, gardenia, gardenia, blackberry, kumazasa, black pine, horseshoe
(Cranberry), cranberry fruit, chestnut or its astringent skin, curculigo-latifolia fruit, grapefruit fruit, buckthorn, black crocodile (rescue), clove (clove,
Dingka), Kaigai (Epispermia japonica), Celosia (cow flower, Chicken corolla), Bayberry (laurel), Gentiana, Gennoshoko (Old grass), Koshinbara (Monthly flower), Kosuihakka (Melissa), Kozo fruit, Kocha (tea), Kouhone
(River bone), kouhon (straw and kara straw), koulyang, koendro fruit, koganebana (yellow gon), lingonberry (koshibachi), coconut fruit, goshyu (goshuyu), goshoichigo (covered bonsai),
Pepper (pepper), copaiba balsam, coffee beans, kobushi tamshiba magnolia (hot spring), burdock (burdock, burdock), kobotdur, sesame (sesame), sesame grassland (wort), wheat (wheat), rice / rice bran ( Red bran, white bran), rice oil,
Cola acuminata seeds, cola vera seeds, fenugreek fruits, colombo, kondurango, kombu, konjac, comfrey (finnari grass), saxima button pulp (weird sen), cherry leaves, flowers, fruits, bark (cherry bark), Pomegranate, Sasa, Sasasa lily (Yuri), Sazanka, Sadomodaka (Sawashatsu), Sweet potato, Sugar cane, Sugar beet, Sunebunt jujube (Acid jujube), Saffron (Bamboo safflower, West safflower), Pomelo fruit, Sabonsou, Salmonberry fruit, Sarashinashoma ( Masu-mae), Salvia (sage), Sawagikyo (Yamakona), Hawthorn (Yamazako), Sanshin ginseng (Sanjin ginseng), Sanshuyu (sanshuyu), Sansho (sansho), Sandskon (Yamazone),
Shea (carite), cyanoki fruit, shiitake (shiitake), shion
(Shiwon), Red squirrel, Shikunshi (Kimiko), Perilla, Aojiso, Chirimenjiso, Catamendiso (Shiyoha, Shiso), Rosewood, Shinanoki, Chinese forsythia, Lily of the valley
(Chrysanthemum flower), Spiraea, Potato, Peony (Shakuyaku), Shajin (Sasan), Janohige (Motomon winter), Juzudama, Palm fruit, Ginger (Ginger), Shobu (Iris, Iris root), Shozuku fruit, Shirakashi seed, White-headed butterfly, white-nosed iris, white-flowered dandelion, white-flowered-flower, white-leaved nanten (minamitenmi), cinchona-sac-silbra, cinnamon, watermelon (watermelon), honeysuckle (gold-silver-flower,
Shinobazu), sorrel (acid model), swimberry fruit, strawberry fruit, horsetail (Thorn), stevia, plum fruit, Scots pine cone, Scots larch, Scots vine, Scotch gross, Scots pear, Scots dandelion, Scots beetle (Maroniae) ), Pear fruit, snow pear, European elderberry, Atlantic spp., Yarrow (milfoil), Balsam pear, European cornflower, European mistletoe,
Mint, horse radish, horseradish, sekisho (stone iris), mallow, hirohasenega,
Senega, senega, seri, celery, senkyu (river cucumber), sendan, senna fruit or leaf, senninso (great tate), senburi (medicine), souka (grass), buckwheat seeds, soybean potato, rhubarb (daihuang), radish , Soybeans, daidai (orange skin, kikomi), takatodai (daigei), takawarabi (gulp),
Dark sweet cherry fruit, hollyhock, hollyhock (thyme, Hyakurika), hollyhock (tachibana skin), hollyhock, tamarind seeds, onion, hamashiba (spicy), arachis or its root bark, tanjin (tansan), dandelion (dankopo), dammaru , Cherry fruit, ginger or its roots (chinese radish), chikusetsu carrot (bamboo ginseng), chicory, ginseng (gomiko), ginseng dai (great yellow), ginseng (Wuyi), ginseng (carrots), ginseng ( Aiba leaf), Choreimaitake (Irei), Tsukimiso, Camellia, Camellia, Spiderweed (lacquer grass), Commelina
(Duck shark), red bean (red azuki bean), red turtle (red bean), red vine (an apricot), red ginseng (four-leaf ginseng), tsuwabuki, daico, teuchigurumi, duberry fruit, tengusa, tendaiyaku (drug), touga (winter melon) , Capsicum (Hot Pepper), Touki (Toki), Calendula (Marigold), Maize or Maize Hair (Southern Hair), Spodoptera (Female Sadako), Toudorindo (Ryubile), Dokudami
(Jujutsu), radish, root ginseng, bamboo ginseng, eucommia, eucalypt, eucalypt, tomato, tragacanth, triacanthos seeds, tolmentilla, dronoki, troroaoi,
Niseria berry fruit, potato yam (Yamayaku),
Nagikadada (Butcher Bloom), Naginata Kouju,
Nazuna, Rape, Natsumikan, Jujube (Dajutsu), Naniwaibara (Kanekurako), Narco lily (Yellow), Peanut (peanut), Nanten (Nantenmi), Nigaki (Makinoki), Nigayomogi (Bittersweet), Nikuzuku, Nikkei ( Cinnamon), Chinese chive (Nirako), elderberry (bone grafted) fruit / flower or foliage, garlic (garlic), nulde (fivefold child), leek, noa thistle (daiji), neubara (natural fruit),
Yarrow, Japanese radish (maehu), Nobara, Nomomo, Palm, Pineapple fruit, Hibiscus (Bussouge, Furin-Bussouge, Roselle), Hakata lily (Yuri), Hakamauraboshi (Bone crushing supplement), Hakusen (Chinese rind), Hakuruberry fruit, Hakobe ( Hazel, hashidokoro
(Roth root), basil, lotus (lotus, lotus), parsley (holland jelly), hadakamugi, batata, hachiku madake (bamboo-boiled), patchouli, mint (lightly loaded, lightly loaded leaves), adlay (yokuinin), hanasuge ( Chichimo), banana, hanamint, vanilla bins, papaya, lycopodium (rat), hub grass,
Paprika, Hamago and Mitsuba Hamago (vine thorns), Hamasuge (Katsubushi), Hamabishi (Situriko), Hamanas (maikai flower), Hamabofu (beach storm), Hamamerisu, rose (rose), Parietaria, harunire (yuhi, yuyu) White skin, yuba),
Breadfruit, Hiougi (sun-dried), Lycopodium carrot (party party), pecan nut, giganbana (stone garlic, mulberry flower),
Hikiokoshi (prolonged life grass), Hishi (Hishimi), pistachio, beet, hitotsuba (stone reed), hinotainokozuchi (cow knee), hinoki, hiba, castor, sunflower, bell pepper, hinimama (incense blossom), himematsutake, pimenta fruit, juniper , Hiyu fruit, below mallow, Hiroha okinagusa (white head), loquat
(Loquat, loquat leaf), betel nut (large clothing skin, betel palm), squash (southern rattan), butterflies and dandelions (winter flowers, winter leaves),
Fujibakamama (Ranbusa), Fujimame (tonboshi), grape fruit or pericarp and leaves, beech, Fuyushinatsukutaketake (Cordyceps sinensis), Brazilian liquorice, black currant fruit, blackberry, plum fruit, flucellaria, blueberry (Acacia catechu), Prunes, blond psyllium, bundo (green beans), loofah, safflower (safflower), belladonna, berry fruit, boysenberry fruit, boufu (windbreak), spinach, physalis (Torone), honoki (thick, sumpaku), bokeh (Gourd), Hosoba-naokera (blue Ajutsu), Hosobabarengiku, Bodaiju (Bodai tree), Button (peony, peony skin),
Hop ears / gland bodies, jojoba, white berries,
Hongshimeji, Meisulten Nanshaw (Tiannan star), Maoh
(Maoh), macadamia nuts, macri (sea grass), maguwa
(Mulberry white skin, mulberry leaves)), madatake (bamboo boiled), matabi (mokuten tate),
Matsukasa, Matsuba, Matsuhodo (Bukuryo), Mayorum (Hanamint), Malvanozadin (Bitterfruit), Mulberry Fruit, Quince, Malonie, Mango, Mangosteen, Mansugurumi, Mandarin Fruit, Mannentake (Lingzhi), Fruits of Citrus Plants (Komimi) , Mishimasaiko (Saiko), Mizokokushi
(Hanbe Lotus), Misohagi (Chisenbana), Mitsugashiwa, Mitsuba, Mitsubahamago (Tsengokuboshi), Midorihakka, Mimosa, Myoga, Miracle Fruit Fruits, Myrrh, Myrobaran, Mukuge (Kiuge), Mukunoki, Mukuroji, Murasaki ( Purple root), purple sorghum, sorghum (meadowgrass), mebaugi, melaleuca, melissa, melilot, melon fruit, moco dandelion, moko mugwort, moso bamboo, mokkou (wood incense), maple leaf, peach leaf (peach leaf), fruit, seed ( Peach), sprouts, morello cherries, moroheiya (burlap), yakawamurasakiimo, yakuchi (masuti), cornflowers (cornflowers), cornflower mint, yashabushi (arrow wheel), yayanagi, yatsude (octagonal metal panel), mistletoe (Willow parasitism), willow leaf, yabugarashi, yabukouji (purple gold beef), pokeweed (land), yamahanoki (mountain) ), Bayberry (Yumebiki), Bayberry, Eucalyptus, Yukinoshita (Tiger Eargrass), Yucca Flebifolia, Yuzu Fruit, Lily, Yoraigusa, Artemisia (Aiba), Lime Fruit, Rye, Lacanca Fruit, Raspberry Fruit, Lavender, Longan ( Longan meat), Ryokucha (green tea), apple fruit, gentian, rubus suavisimus (sweeten), lettuce, red currant fruit, lemon fruit, lemongrass, forsythia (forsythia),
Astragalus, wax palm, loganberry fruit, log wood, rosemary (mannen wax), rose hip (nobara), seaweed, wasabi, waremokou (chiyu) and the like.

[0033] Materials derived from animal raw materials include cockscomb extract, bovine / human placenta extract, swine / cattle stomach, duodenum or intestinal extract or decomposed product thereof, and swine / cattle spleen extract. Products or their degradation products, extracts of swine or bovine brain tissue, water-soluble collagen, collagen derivatives such as acylated collagen, collagen hydrolysates, elastin, elastin hydrolysates, water-soluble elastin derivatives, keratin and its decomposition products Or a derivative thereof, a silk protein and its degradation product or a derivative thereof, a pig / bovine blood cell protein degradation product (globin peptide), a pig / bovine hemoglobin degradation product (hemin, hematin, heme, protohem, heme iron, etc.), milk , Casein and its decomposition products or their derivatives, skim milk powder and its decomposition products or their derivatives, lactoferrin or its decomposition products, chicken egg ingredients Such as fish meat decomposition products.

(14) Microbial culture metabolites Yeast metabolites, yeast extract, fermented rice extract, fermented rice bran extract, euglena extract, lactic acid fermented product of skim milk powder, trehalose or derivatives thereof.

(15) α-hydroxy acid Glycolic acid, citric acid, malic acid, tartaric acid, lactic acid and the like.

(16) Inorganic pigments Silicic anhydride, magnesium silicate, talc, kaolin,
Bentonite, mica, titanium mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide,
Titanium oxide, calcium carbonate, magnesium carbonate, yellow iron oxide, red iron oxide, black iron oxide, gunjou, chromium oxide,
Chromium hydroxide, carbon black, calamine, etc.

(17) UV absorber p-aminobenzoic acid derivative, salicylic acid derivative, anthranilic acid derivative, coumarin derivative, amino acid compound,
Benzotriazole derivatives, tetrazole derivatives, imidazoline derivatives, pyrimidine derivatives, dioxane derivatives, camphor derivatives, furan derivatives, pyrone derivatives,
Nucleic acid derivatives, allantoin derivatives, nicotinic acid derivatives,
Vitamin B ₆ derivatives, oxybenzone, benzophenone, guaiazulene, shikonin, baicalin, baicalein, berberine and the like.

(18) Astringent succinic acid, allantoin, zinc chloride, zinc sulfate, zinc oxide, calamine, zinc p-phenolsulfonate, potassium aluminum sulfate, resorcinol, ferric chloride, tannic acid (including catechin compounds) Such.

(19) Antioxidants ascorbic acid and its salts, stearic acid esters, tocopherol and its ester derivatives, nordihydroguaseletenic acid, butylhydroxytoluene (BHT),
Butylhydroxyanisole (BHA), hydroxytyrosol, parahydroxyanisole, propyl gallate, sesamol, sesamolin, gossypol and the like.

(20) Anti-inflammatory agent Ictamole, indomethacin, kaolin, salicylic acid, sodium salicylate, methyl salicylate, acetylsalicylic acid, diphenhydramine hydrochloride, d or dl-
Camphor, hydrocortisone, guaiazulene, camazulene, chlorpheniramine maleate, glycyrrhizic acid and its salts, glycyrrhetinic acid and its salts and the like.

(21) Disinfectant / Disinfectant Acrinol, sulfur, benzalkonium chloride, benzethonium chloride, methylrosaniline chloride, cresol, calcium gluconate, chlorhexidine gluconate, sulfamine, mercurochrome, lactoferrin or a hydrolyzate thereof.

(22) Humidifier Glycerin, propylene glycol, 1,3-butylene glycol, hyaluronic acid and its salts, polyethylene glycol, chondroitin sulfate and its salts, water-soluble chitin or chitosan derivatives, sodium lactate and the like.

(23) Hair preparations Selenium disulfide, alkylisoquinolinium bromide solution, zinc pyrithione, biphenamine, thianthol, castal tincture, ginger tincture, capsicum tincture, quinine hydrochloride, strong aqueous ammonia, potassium bromate, bromate Sodium, thioglycolic acid, etc.

(24) Flavors Natural animal flavors such as musk, civet, castorium, ambergris, anise essential oil, angelica essential oil, ylang-ylang essential oil, iris essential oil, fennel essential oil, orange essential oil, cananga essential oil, caraway essential oil, cardamom essential oil, Guaiac wood essential oil, cumin essential oil, black letter essential oil, cinnamon essential oil, cinnamon essential oil, geranium essential oil, copaiba balsam essential oil, coriandel essential oil, perilla essential oil, cedarwood essential oil, citronella essential oil, jasmine essential oil, ginger grass essential oil, cedar essential oil, spearmint essential oil, western Mentha essential oil, Great Fengxiang essential oil, Tuberose essential oil, T-shaped essential oil, Orange flower essential oil, Winter green essential oil, Truval sam essential oil, Batturi essential oil, Rose essential oil, Palmarosa essential oil, Cypress essential oil, Hiba essential oil,
Sandalwood essential oil, Petitgren essential oil, Bay essential oil, Vetiva essential oil, Bergamot essential oil, Peruvian balsam essential oil, Boad rose essential oil, Camphor essential oil, Mandarin essential oil, Eucalyptus essential oil, Lime essential oil, Lavender essential oil, Linaloe essential oil, Lemongrass essential oil, Lemon essential oil, Rosemary Vegetable flavors such as essential oils and Japanese peppermint essential oils, and other synthetic flavors.

(25) Dyes / colorants Red cabbage dye, red rice dye, madder dye, anato dye, squid dye, turmeric dye, orange dye, krill dye, persimmon dye, caramel, gold, silver, gardenia dye, corn dye , Onion pigment, tamarind pigment, spirulina pigment, buckwheat whole plant pigment, cherry pigment, laver pigment, hibiscus pigment, grape juice pigment, marigold pigment, purple potato pigment, purple yam pigment, lac pigment, rutin, etc.

(26) Sweeteners Sugar, sweet tea, fructose, arabinose, galactose, xylose, mannose, maltose, honey, glucose, miraculin, monelin, licorice extract and the like.

(27) Nutrition enhancer Shell calcined calcium, cyanocorabamine, yeast, wheat germ, egg yolk powder, hemicellulose, heme iron and the like.

(28) Dairy products Milk, cheese, fresh cream, butter, margarine, powdered milk, whey, condensed milk and the like.

In addition, humectants, hormones, sequestering agents, pH adjusters, chelating agents, antiseptic / antibacterial agents, cooling agents, stabilizers, emulsifiers, animal and vegetable proteins and their decomposed products, animal and vegetable Vegetable polysaccharides and their degradation products, animal and vegetable glycoproteins and their degradation products, blood flow enhancers, anti-inflammatory and anti-allergic agents, cell activators, keratolytic agents, wound healing agents, foam enhancers, thickeners Agents, oral preparations, deodorants / deodorants, bitters, seasonings, enzymes, and the like can be used, and additive and synergistic effects can be expected by using them in combination.

The fibroblast proliferation-promoting agent of the present invention, the external preparation for skin, the bath preparation, and the food and drink can be in any form, such as ampules, capsules, powders, granules, pills and tablets. , Pharmaceuticals such as solid, liquid, gel, foam, emulsion, cream, ointment, sheet, etc., quasi-drugs,
It can be used by blending it into cosmetics for skin and hair, bath preparations and foods and drinks.

[0051] Specifically, for example, pharmaceutical preparations for internal or external use, lotions, emulsions, creams, ointments, lotions, oils, basic cosmetics such as packs, facial cleansers and skin cleansers, shampoos, rinses, hair treatments , Hair creams, pomades, hair sprays, hair styling products, perm agents, hair tonics, hair dyes, hair growth and hair growth products, and other hair cosmetics
Makeup cosmetics such as foundation, white powder, whitening, lipstick, blusher, eyeshadow, eyeliner, mascara, eyebrows, eyelashes, etc.
Includes perfumes, bath agents, other toothpastes, mouth fresheners / gargles, liquid odor / deodorant inhibitors, sanitary products, sanitary cotton, wet tissues, etc., and also used in general foods and drinks It is possible.

The method for adding the fibroblast proliferation promoter, skin external preparation and bath preparation, and food and drink of the present invention is as follows.
It may be added in advance or may be added during the production, and may be appropriately selected in consideration of workability.

[0053]

EXAMPLES The present invention will now be described with reference to production examples, test examples, and prescription examples, but the present invention is not limited to these examples.

(Production Example 1) Purified water 20 g
The mixture is extracted by heating at 0 mL (about 80 ° C.) for about 4 hours, and filtered by suction to obtain about 1 kg of an extract (dry solid content: about 0.01 to 1.5% by weight).

(Production Example 2) Chlorella bulk powder (100 g) was purified water (20).
After heating and extracting at 0 mL (about 90 ° C) for about 2-3 hours, 0.22 μm
The resulting extract is dried by aseptic filtration with a filter (manufactured by Toyo Paper), and about 10.0 g of powder (dry solid content: about 0.01 to 1.5% by weight) is obtained.

(Production Example 3) 100 g of aloe vera leaves were pulverized, squeezed, extracted and filtered, ethanol was added to the obtained filtrate, and the mixture was stirred. The resulting precipitate was collected, and ethanol was added again, washed, and then obtained. Ethanol was removed from the obtained washing solution under reduced pressure, and the obtained solid content (extract) was dissolved in purified water, and the extract solution (dry solid content: about 0.05 to 1.0% by weight) was added to about 1.0% by weight.
get g.

(Production Example 4) After crushing 100 g of aloe vera leaves, 1,000 mL of purified water or 1,000 mL of a 30% ethanol solution was added.
Alternatively, the mixture is extracted with 1,000 mL of a 40% 1,3-butylene glycol solution or 1,000 mL of propylene glycol extraction for about 5 to 6 days, filtered, and the extract is dried to obtain a powder (dry solid content: about 0.2%).
005-2.0 ^w / _v %).

(Test 1) Fibroblast proliferation promotion test The present inventor used MTT based on normal fibroblasts of the skin.
Using the reduction method, the effect of promoting cell growth was tested and evaluated.

(Test Method) The MTT reduction method is a method for evaluating cytotoxicity and cell activation (rapid color quantification of growing and surviving cells: Tim Mosmann; Journal of Immunological Met).
hods p55-63 (1983)) and MTT (3- (4,
5-dimethyl-2-thiazolyl) -2,5-diphenyltetrazolium br
omide) is taken up by cells, is reduced and cleaved by NADH present in mitochondria in the cells, and utilizes the property of producing water-insoluble blue formazan.This blue formazan is dissolved in acidic isopropanol, and the absorbance is measured. It is to be measured. That is, since the amount of blue formazan is expressed as the absolute amount of mitochondria, the amount of blue formazan that changes over time was measured, and the cell proliferation rate was calculated.

(Quantification of Fibroblast Proliferation-Promoting Effect) The present inventor has proposed that FBS in which cells do not grow or decrease in advance.
(Fetal calf serum) The concentration was examined and MEM containing 0.5% FBS
It was confirmed that this condition was satisfied when cultured in a medium, and the serum concentration of the test medium was adjusted to 0.5%, and it was decided that the fibroblast proliferation promoting effect of only the test sample could be quantitatively evaluated.

(Measurement of fibroblast proliferation promoting action) 5% F
Using a MEM medium containing BS (fetal calf serum), human dermal fibroblasts (NB1RGB purchased from RIKEN Genebank / Cell Development Bank) at a density of 5 × 10 ³ Cell / Well for 96 Wells pl
ate and cultured for 24 hours under the conditions of 5% CO ₂ and 37 ° C.
Then, samples of each concentration (Production Example 1: Chlorella water extract,
Production Example 3: Aloe vera extract, Production Examples 1 and 3: Chlorella water extract and Aloe vera extract)
The medium was replaced with a MEM medium containing BS. At this time, a blank was used as a MEM medium containing 0.5% FBS containing no sample, and culturing was continued in a CO ₂ incubator for 9 days while changing to the same medium every 3 days.
The absorbance difference of nm was measured, and the absorbance difference of the blank was set to 100 at this time, and the cell growth rate of the test sample-added group with respect to the difference was measured.

[0062]

FIG.

(Test Results) As shown in FIG. 1, it was confirmed that the combined use of the chlorella water extract and the aloe vera extract of the present invention had a synergistic effect on the fibroblast proliferation promoting effect.

(Test 2) Safety test (1) Primary skin irritation test The extracts and powders obtained in Production Examples 1 to 4 were prepared with purified water so that the dry solid concentration was about 0.5% by weight. Japanese rabbits with female back shaved (female, 3 per group, weight 2.3kg)
Before and after). The judgment was made 24, 48, and 72 hours after application by the primary irritation scoring method using erythema and edema as indices. The result was negative in all animals without any erythema and edema.

(Test 3) Safety Test (2) Skin Cumulative Irritation Test Similarly, the extracts and powders obtained in Production Examples 1 to 4 were added to purified water so that the dry solid concentration was about 0.5% by weight. Guinea pigs (female,
Once a day, 5 times a week on the skin of 3 animals per group, weight around 320g)
0.5 ml / animal was applied. Application was performed over two weeks and shaving was performed on the last application day of each week. Judgment was made on the day of each application and on the day after the last application using the primary irritancy scoring method using erythema and edema as indices. As a result, all animals were judged as negative without any erythema and edema over 2 weeks.

(Test 4) Safety test (3) Acute toxicity test Similarly, the powder (dry solid content of about 0.5
Weight percent) of chlorella water-extracted powder and aloe vera extract powder were orally administered to ddy mice (male and female, 5 mice / group, 5 weeks old), which had been fasted for 4 hours before the test, in an amount of 2,000 mg / kg.
The appearance and degree of toxicity symptoms were observed over time. As a result, no abnormality was observed in all mice for 14 days,
In addition, there was no abnormality in the dissection result. Therefore, LD ₅₀ is 2,00
It was determined to be 0 mg / kg or more.

(Prescription examples) Production of various fibroblast growth agents, skin external preparations and bath agents The following formulation examples are shown below according to the above evaluation results, and each formulation example is produced by a conventional method for producing each product. And only the amount was shown. Further, the present invention is not limited to these.

(Formulation Example 1) Emulsion weight% 1. Squalane 5.0 2. Olive oil 5.0 3. Jojoba oil 5.0 4. Cetyl alcohol 1.5 5. Glycerin monostearate 2.0 6. Polyoxyethylene (20) cetyl ether 3.0 7. Poly Oxyethylene (20) soorbitan monooleate 2.0 8.1,3-butylene glycol 1.0 9. Glycerin 2.0 10. Chlorella hot water extract (solid content 0.3%) 3.0 11. Aloe vera water extract solution (solid content 0.3%) 3.0 12 Appropriate amount of fragrance and preservatives 13. Residual water with purified water of 100

(Formulation Example 2) Lotion weight% 1. Sorbit 2.0 2.1.3-butylene glycol 2.0 3. Polyethylene glycol 1000 1.0 4. Polyoxyethylene oleyl ether (25E.O.) 2.0 5. Ethanol 10.0 6. Chlorella water Extract (solid content 0.3%) 3.0 7. Aloe vera 30% ethanol extract (solid content 0.2%) 3.0 8. pH adjuster qs 9. Preservative qs 10. Purified water 100 residue

(Formulation Example 3) Peel-off pack weight% 1. Glycerin 5.0 2. Propylene glycol 4.0 3. Polyvinyl alcohol 15.0 4. Ethanol 8.0 5. Polyoxyethylene glycol 1.0 6. Chlorella hot water extract (solid content 0.8%) 3.0 7. Aloe vera hot water extract (solid content 0.4%) 3.0 8. Appropriate amount of fragrance and preservative 9. Purified water 100

(Formulation Example 4) Cold cream weight% 1. Salicone beeswax 11.0 2. Liquid paraffin 22.0 3. Lanolin 10.0 4. Almond oil 15.0 5. Borax 0.5 6. Chlorella hot water extract (solid content 0.5%) 3.0 7. Aloe vera 50% 1,3-butylene glycol extract (solid content 1.0%) 3.0 8. Appropriate amount of fragrance and preservative

(Formulation Example 5) Shampoo weight% 1. Triethanolamine lauryl sulfate 5.0 2. Sodium polyoxyethylene lauryl ether sulfate 12.0 3.1,3-butylene glycol 4.0 4. Diethanolamide laurate 2.0 5. Disodium edetate 0.1 6. Chlorella hot water extract (solid content 0.7%) 5.0 7. Aloe vera 50% phenolic percoll extract (solid content 0.7%) 5.0 8. Appropriate perfume and preservative 9. Purified water 100

(Formulation Example 6) Body soap weight% 1. Potassium laurate 15.0 2. Potassium myristate 5.0 3. Propylene glycol 5.0 4. Chlorella aqueous extract (solid content 0.3%) 5.0 5. Aloe vera aqueous extract (solid 0.3%) 3.0 6. Appropriate amount of pH adjuster 7. Appropriate amount of preservative

(Formulation Example 7) Rinse weight% 1. Stearyltrimethylammonium chloride 2.0 2. Cetostearyl alcohol 2.0 3. Polyoxyethylene lanolin ether 3.0 4. Propylene glycol 5.0 5. Aloe vera water extract (solid content 0.5%) 3.0 6. Chlorella water extract (solid content 0.4%) 5.0 7. Appropriate amount of pH adjuster 8. Appropriate amount of preservative 9. Residue to 100 purified water

(Formulation Example 8) Hair liquid% by weight 1. Ethanol 29.0 2. Polyoxypropylene butyl ether phosphoric acid 10.0 3. Polyoxypropylene monobutyl ether 5.0 4. Triethanolamine 1.0 5. Chlorella hot water extract (solid content 0.2 5.0) 6. Aloe vera anhydrous ethanol extract (solid content 0.4%) 3.0 7. Appropriate amount of preservative 8. Purified water 100

(Formulation Example 9) Hair tonic weight% 1. Ethanol 40.0 2. Ethyl oleate 1.0 3. Polyoxyethylene (40) hydrogenated castor oil 2.0 4. Chlorella hot water extract (solid content 0.5%) 5.0 5. Aloe vera pressed extract (solid content 0.5%) 3.0 6. Purified water 100 residue

(Formulation Example 10) Granule bath agent weight% 1. Sodium bicarbonate 61.0 2. Anhydrous sodium sulfate 35.0 3. Borax 3.0 4. Chlorella hot water extraction powder (solid content 1.5%) 3.0 5. Aloe vera pressed extraction powder (Solid content 1.0%) 3.0

(Formulation Example 11) Coating protective agent A gauze or liniment cloth is impregnated with a formulation solution obtained by mixing an appropriate amount of aloe vera / chlorella hot water mixed extract (solid content: 1.0%), antibiotics, anti-inflammation, etc. Attach. Alternatively, the chlorella extract may be directly sprayed locally and covered with gauze or the like.

(Formulation Example 12) Juice drink weight% 1. Glucose liquid sugar 33.0 2. Grapefruit juice 63.0 3. Fragrance 0.5 4. Chlorella water extract powder (solid content 1.0%) 3.0 5. Aloe vera hot water extract (solid content) 1.0%) 3.0 6.Sour seasoning

(Formulation Example 13) Mentsuyu liquid% by weight 1. Soy sauce 76.0 2. Black vinegar 2.0 3. Glucose 15.0 4. Sodium glutamate 2.0 5. Chlorella water extraction powder (solid content 1.5%) 3.0 6. Aloe vera water extraction powder ( (Solid content 2.0%) 3.0

(Formulation Example 14) Udon or buckwheat wt% 1. Flour or buckwheat flour 93.0 2. Salt 1.0 3. Chlorella water extract (solid content 1.6%) 3.0 4. Aloe vera water extract (solid content 1.5%) 3.0

(Formulation Example 15) Bread wt% 1. Wheat flour 90.0 2. Salt 0.5 3. Glucose 6.5 4. Chlorella hot water extract powder (solid content 1.5%) 1.5 5. Aloe vera water extract powder (solid content 1.0%) 1.5

(Formulation Example 16) Cookie weight% 1. Milk 62.0 2. Whole egg 14.0 3. Sugar 15.0 4. Constarch 5.0 5. Salt 0.6 6. Chlorella hot water extraction powder (solid content 1.0%) 1.7 7. Aloe vera water Extracted powder (solid content 1.0%) 1.7 8.Perfume

(Test 5) Use Effect Test The effect of a skin external preparation and a bath preparation containing the essential components (chlorella water extract and aloe vera extract) of the present invention was examined. The test was conducted with 20 panelists aged 5 to 50 years old who suffer from skin diseases such as dry skin, rough skin, eczema, and atopic dermatitis.
The washing was performed twice a day, in the morning and at night, by applying an appropriate amount of the emulsion of Formulation Example 1 to the face over three months after washing the face. In addition, 20 people (5 to 5) who have the same skin disease on the scalp or hairline
(0 year old) was also subjected to a use test by applying an appropriate amount of the hair tonic of Formulation Example 9 to the scalp over 2 months after daily shampooing.

Further, bath preparations containing the essential components (chlorella water extract and aloe vera extract) of Formulation Example 10 also include skin diseases such as dry skin, rough skin, eczema, and skin inflammation such as atopic dermatitis. For 20 persons aged 1 to 65 years old who were worried about, they were always bathed once a day for 3 months in a bath with an appropriate amount of bathing agent dissolved therein, and a use test was conducted.

[0086] Comparative controls were the emulsions, hair tonics, and bath preparations except for the essential components (chlorella water extract and aloe vera extract), the chlorella water extract only, and the aloe vera extract only. Those prepared by the same method were used. The evaluation was performed according to the following criteria. The results are as shown in Table 1 and the numerical values in the table represent the number of persons. No one complained of abnormalities on the skin or scalp during the use period.

"Effect of improving skin (scalp) disease" Efficacy: Redness, itching, dry skin and rough skin due to inflammation such as eczema were improved. Somewhat effective: Redness and itching, dry skin, and rough skin due to inflammation such as eczema were slightly improved. Ineffective: No change from before use.

(Test Results) As shown in Table 1, the use of a skin external preparation and a bath containing the chlorella water extract and the aloe vera extract of the present invention resulted in skin diseases such as inflammation due to eczema, itching, dry skin and rough skin. , A good effect was confirmed.

[0089]

[Table 1]

(Test 6) Use Effect Test The effect of a food containing the essential components of the present invention (chlorella water extract and aloe vera extract) was actually examined. The test used is to worry about dry skin, rough skin, eczema, atopic dermatitis and other skin diseases.
A panel of 10 50-year-olds, twice daily, morning and night,
Five cookies of Formulation Example 16 were drunk for three months.

Controls include those obtained by removing the essential components (chlorella water extract and aloe vera extract), only the chlorella water extract from the cookies, those obtained by removing the aloe vera extract only, and the essential components (chlorella water extract and aloe vera extract) from the cookies. (Extract) was formulated in a similar manner. The evaluation was performed according to the following criteria. The results are as shown in Table 2, and the numerical values in the table represent the number of persons. No one complained of abnormalities during the use period.

"Skin (scalp) disease improving effect" Efficacy: Redness, itching, dry skin and rough skin due to inflammation such as eczema were improved. Somewhat effective: Redness and itching, dry skin, and rough skin due to inflammation such as eczema were slightly improved. Ineffective: No change from before use.

(Test Results) As shown in Table 2, the use of the food containing the water extract of chlorella and the extract of aloe vera of the present invention is effective in improving skin diseases such as inflammation due to eczema, itching, dry skin and rough skin. , Good effect was confirmed.

[0094]

[Table 2]

[0095]

EFFECT OF THE INVENTION It is clear that the essential components of the present invention (chlorella water extract and aloe vera extract) have a remarkable action of promoting fibroblast proliferation by normal cells, and furthermore do not show cytotoxicity. Or it is safe to use internally or externally for animals. Therefore, it can be used as an agent for promoting fibroblast proliferation, and for the purpose of promoting skin diseases such as eczema, rough skin, dry skin, atopic dermatitis and skin and dermis development, preventing skin aging, and promoting wound healing. It is effective for prevention and treatment of various traumas such as skin, scalp, oral cavity, eyes, ears, nose, anus, and genital area. Furthermore, it can be applied to all forms of preparations (pharmaceuticals, quasi-drugs, cosmetics, foods), and if used as an external preparation for skin and bath or food or drink, inflammation due to eczema,
It can also be used for the prevention and improvement of skin and scalp such as itching, rough skin and rough skin.

[Brief description of the drawings]

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 shows the relationship between the essential components (chlorella water extract and aloe vera extract) of the present invention and the concentration in the medium and the cell growth rate when a chlorella water extract or aloe vera extract is added. .

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[Procedure amendment]

[Submission date] August 27, 1996

[Procedure amendment 1]

[Document name to be amended] Statement

[Correction target item name] 0077

[Correction method] Change

[Correction contents]

(Formulation Example 10) Granule bath agent weight% 1. Sodium bicarbonate 61.0 2. Anhydrous sodium sulfate 30.0 3. Borax 3.0 4. Chlorella hot water extraction powder (solid content 1.5%) 3.0 5. Aloe vera pressed extraction powder (Solid content 1.0%) 3.0

──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. ⁶ Identification number Office reference number FI Technical display location A23L 1/30 A23L 1/30 B A61K 7/00 A61K 7/00 K 7/06 7/06 7 / 075 7/075 7/08 7/08 7/48 7/48 7/50 7/50 35/78 ADA 35/78 ADAV // (A61K 35/80 35:78)

Claims (3)

[Claims] An agent for promoting fibroblast proliferation, comprising the following (1) and (2) as essential components: (1) Chlorella water extract. (2) Aloe vera extract. 2. An external preparation for skin and a bath preparation, which comprises the fibroblast proliferation promoter according to claim 1. (3) A food or drink comprising the fibroblast proliferation promoter according to (1).