JP2004123637A - Hyaluronic acid production promoter - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To develop a hyaluronic acid production promoter, promoting the production of hyaluronic acid, and effective for prevention of skin aging such as amelioration of rough skin, impartment of elasticity, tenseness and gloss to the skin, and amelioration of wrinkle and slack; and to obtain a composition for inducing beauty and homeostasis maintenance of an organism by a natural action of the organism by utilizing the promoter. <P>SOLUTION: This hyaluronic acid production promoter contains a collagen peptide (Gly-X-Y)<SB>n</SB>(wherein, Gly is a glycine residue; X and Y are each an amino acid residue; and n is a positive integer) which is a degradation product of a collagen or a gelatin by a collagenase, and comprises a collagen tripeptide having an amino acid sequence represented by (Gly-X-Y). A skin care preparation for external use and a food and drink product contain the hyaluronic acid production promoter. As a result, the production of the hyaluronic acid is significantly promoted, and rough skin-ameliorating activities are provided. The skin care preparation for external use and the food and drink product containing the promoter have the skin aging-preventing effects such as the impartment of the tenseness and gloss, and the amelioration of the wrinkle and the slack. <P>COPYRIGHT: (C)2004,JPO

Description

【００９４】
〈表１〉

【００９５】
表１の通り、本発明のコラーゲンペプチドは、肌荒れ改善に有効であると認められた。
【００９６】
（試験３）安全性試験
（１）皮膚一次刺激性試験
製造例１で得られたコラーゲンペプチドを固形分濃度が約２．０％になるように調製し、これを背部を剪毛したハートレー系モルモット（雌性，１群３匹，体重３００〜３５０ｇ）の皮膚の２×２ｃｍ^２の四角当たり０．０３ｍＬずつ塗布した。判定は、適用後２４，４８，７２時間に一次刺激性の評点法により紅斑及び浮腫を指標として行った。その結果、全ての動物において、何等、紅斑及び浮腫を認めず陰性と判定された。
【００９７】
（試験４）安全性試験
（２）皮膚累積刺激性試験
製造例１で得られたコラーゲンペプチドを固形分濃度が約２．０％となるように調製し、これを側腹部を剪毛（２×４ｃｍ^２）したハートレー系モルモット（雌性，１群３匹，体重３２０ｇ前後）の皮膚に１日１回、週５回、０．５ｍＬ／動物当りを塗布した。塗布は２週にわたって、又、剪毛は各週の最終塗布日に行った。判定は、各塗布日及び最終塗布日の翌日に一次刺激性の評点法により紅斑及び浮腫を指標として行った。その結果、全ての動物において、塗布後２週間にわたって、何等、紅斑及び浮腫を認めず陰性と判断された。
【００９８】
（試験５）安全性試験
（３）単回投与毒性試験
製造例１得られたコラーゲンペプチドを１２．５％濃度になるよう精製水にて調整し、一匹につき１６ｍＬ／ｋｇ量を、試験前、１６時間絶食させたｄｄｙ系マウス（雄性及び雌性、１群５匹、５週齢）に経口にてゾンデを以て投与し、毒性症状の発現、程度等を経時的に観察した。その結果、全てのマウスにおいて１４日間、何等異常を認めず、解剖の結果も異常がなかった。よって、ＬＤ_５０は２，０００ｍｇ／ｋｇ以上であり、安全な物質と判断した。
【００９９】
（処方例）ヒアルロン酸産生促進剤、皮膚外用剤及び飲食品の製造
上記の評価結果に従い、以下にその処方例を示すが、各処方例は各製品の製造における常法により製造したもので良く、含有量のみを示した。又、本発明はこれらに限定されるわけではない。
【０１００】

【０１０１】

【０１０２】

【０１０３】

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【０１０５】

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【０１０７】

【０１０８】

【０１０９】

【０１１０】

【０１１１】

【０１１２】

【０１１３】

【０１１４】

【０１１５】

【０１１６】

【０１１７】

【０１１８】

【０１１９】

【０１２０】

【０１２１】

【０１２２】

【０１２３】

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【０１２７】

【０１２８】
（処方例２９）被覆保護剤
ガーゼ又はリニメント布に製造例１のブタ由来コラーゲンペプチド及びサメ由来コラーゲンペプチド、抗生物質、抗炎症剤等、適量を混合した処方液を含浸させ、外傷部に添付する。又、製造例１のブタ由来コラーゲンペプチド及びサメ由来コラーゲンペプチドを直接、局所に散布し、ガーゼ等で被覆しても良い。
【０１２９】

【０１３０】

【０１３１】

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【０１３９】
（試験６）使用効果試験
本発明の皮膚外用剤を実際に使用した場合の効果について検討を行った。使用テストは健康な成人女性２５〜５０歳の１０名をパネラーとし、毎日、朝と夜の２回、洗顔後に処方例１の乳液の適量を顔面に３ヶ月に渡って塗布することにより行った。尚、対照には、乳液から本発明のコラーゲンペプチドを除いたものを同様な方法にて処方したものを用いた。又、評価方法は下記の基準にて行い、結果は表２の通りで表中の数値は人数を表す。尚、使用期間中に皮膚の異常を訴えた者はなかった。
【０１４０】
「肌の張り・ツヤ改善効果」
有　　効：肌の張り・ツヤが増した。
やや有効：肌の張り・ツヤがやや増した。
無　　効：使用前と変化なし。
【０１４１】
〈表２〉

【０１４２】
（試験結果）
表２の通り、本発明品を含有する乳液は、有意に肌の張り・ツヤを与え、又、肌のシワ軽減等の効果を有することが確認できた。
【０１４３】
（試験７）使用効果試験
本発明の食品を実際に使用した場合の効果について検討を行った。使用テストは健康な成人女性２５〜５０歳の１０名をパネラーとし、毎日、朝と夜の２回、処方例３９のクッキー３枚（１回約５０ｇ）を１ヶ月に渡って飲食してもらった。尚、対照には、クッキーから本発明のコラーゲンペプチドを除いたものを同様な方法にて処方したものを用いた。又、評価方法は下記の基準にて行い、結果は表３の通りで表中の数値は人数を表す。尚、使用期間中に皮膚の異常を訴えた者はなかった。
【０１４４】
「肌の張り・ツヤ改善効果」
有　　効：肌の張り・ツヤが増した。
やや有効：肌の張り・ツヤがやや増した。
無　　効：使用前と変化なし。
【０１４５】
〈表３〉

【０１４６】
（試験結果）
表３の通り、本発明品を含有するクッキーは、有意に肌の張り・ツヤを与え、又、肌のタルミも軽減することが確認できた。
【０１４７】
【発明の効果】
本発明のコラーゲン又はゼラチンのコラゲナーゼによる分解物であり、そのアミノ酸配列が、（Ｇｌｙ−Ｘ−Ｙ）（式中、Ｇｌｙは、グリシン残基を表し、Ｘ，Ｙはアミノ酸残基を表し、互いにＸ，Ｙは同一であっても、異なってもよい）で表されるコラーゲントリペプチドからなるコラーゲンペプチド（Ｇｌｙ−Ｘ−Ｙ）ｎ（ｎは、正の整数を表す）は、安全性が高く、ヒアルロン酸産生を有意に促進し、肌荒れ改善作用を有することが確認された。更に、これらを含有する皮膚外用剤や飲食品は肌に対して、張りやツヤの付与、又はシワやタルミの改善といった皮膚老化防止効果を有する。
【図面の簡単な説明】
【図１】本発明のブタ由来コラーゲンペプチド（「サメ由来ＣＰ」と表す）並びにサメ由来コラーゲンペプチド（「ブタ由来ＣＰ」と表す）のヒアルロン酸産生促進率を示す図である。[0001]
TECHNICAL FIELD OF THE INVENTION
The present invention provides a hyaluronic acid production promoter containing, as an active ingredient, a specific collagen peptide which is a decomposition product of collagen or gelatin by collagenase, and a skin external preparation or a food or drink containing the same.
[0002]
[Prior art]
The typical changes in the aging of human skin are wrinkles and lumps, which are one of the major worries for older people and women. However, at present, there are few effective treatments for skin wrinkles and tarmi, and various studies on aging are beginning to take place in an aging society.
[0003]
At present, it is thought that the formation of wrinkles and tarmi on human skin is caused by a decrease in the elasticity of the skin, and the factors include aging, drying, oxidation, and the effects of sunlight (ultraviolet rays). Specific histological phenomena include changes in extracellular matrix components such as collagen, elastin, and glycosaminoglycans in the dermis, and among these glycosaminoglycans, hyaluronic acid is particularly widely distributed in connective tissues. It is known as a high molecular weight polysaccharide that retains a large amount of water in the skin and is in a gel form. Therefore, qualitative and quantitative reduction of hyaluronic acid is considered to be important in skin aging.
[0004]
[Problems to be solved by the invention]
Therefore, the present invention promotes the production of hyaluronic acid, develops a hyaluronic acid production promoter effective for preventing skin aging such as improving skin roughness, imparting skin elasticity, tension, and luster, and improving wrinkles and tallness. It is an object of the present invention to provide a composition for utilizing the natural action of a living body to lead to beauty and maintenance of homeostasis.
[0005]
[Means for Solving the Problems]
In view of these circumstances, the present inventors have made the development theme a useful natural component having a hyaluronic acid production promoting action, and as a result, it is a degradation product of collagen or gelatin by collagenase, and its amino acid sequence is (Gly- XY) (wherein Gly represents a glycine residue, X and Y represent amino acid residues, and X and Y may be the same or different from each other) It was confirmed that collagen peptide (Gly-XY) n (n is a positive integer) consisting of the following significantly promotes hyaluronic acid production. In addition, by increasing the amount of hyaluronic acid distributed in the connective tissue, it has an effect of improving skin roughness, and external preparations and foods and drinks containing these, for the skin, imparting tension and luster, or improving wrinkles and tarmi It was also confirmed that it had an effect of preventing skin aging. Therefore, the present invention has been completed by providing a novel and safe hyaluronic acid production promoter, a skin external preparation, and food and drink.
[0006]
BEST MODE FOR CARRYING OUT THE INVENTION
The active ingredient of the present invention, collagen, which is a starting material for collagen peptide, is not particularly limited, and any of type I to type XIII collagen can be used. Collagen can also be used. In reality, it is assumed that mixed collagen obtained from various animals and fishes is used as collagen. However, the source of this collagen is animal (eg, cow, pig, bird, whale, etc.). And fish (eg, flounder, salmon, sardine, tuna, shark, etc.). Of these, pigs and sharks are particularly preferred.
[0007]
Extraction and purification of collagen from these components can be performed using a generally known method. Specifically, for example, after crushing a tissue containing collagen such as bone, skin, tendon, meat, daffodils and scales, washing with water, extraction with a dilute salt solution, extraction with an acid or alkali solution, pepsin, trypsin and hyaluronidase The collagen can be obtained by extracting with an enzyme such as, and subjecting it to a known purification means such as salting out or dialysis to purify collagen. It is also possible to obtain “regenerated collagen” by a generally known method. In addition, commercially available collagen can be used as a starting material for a collagen peptide.
[0008]
Gelatin is a water-soluble protein obtained by heating and extracting the above-mentioned collagen with water. In the present invention, gelatin produced by a generally known method can be used as a starting material for the collagen peptide, and a commercially available product can also be used.
[0009]
The collagen peptide as an active ingredient of the present invention can be produced by allowing collagenase to act on collagen or gelatin obtained as described above.
[0010]
The collagenase used here is not particularly limited, but is derived from a bacterium such as Clostridium histolyticum Streptomyces parvulus, an actinomycete or a fungus, and has an amino acid sequence unique to collagen [(Gly-XY) n (where Gly is glycine residue). X and Y represent amino acid residues, which may be the same or different from each other, and n represents a positive integer): Hereinafter, this amino acid sequence is also referred to as “unique amino acid sequence” )]), It is preferable to use a collagenase that specifically cleaves the amino group terminal side of the glycine residue, since it is possible to obtain a collagen peptide rich in a peptide having this unique amino acid sequence.
[0011]
The collagenase used here may be, of course, a collagenase obtained as a natural product, for example, a modified collagenase having the above-mentioned specificity, which is obtained by being modified by a protein engineering technique.
[0012]
The types of amino acid residues that X and Y can take are not particularly limited, and are usually amino acid residues of naturally occurring amino acids, specifically, alanine residues, valine residues, and leucine residues. Group, isoleucine residue, proline residue, hydroxyproline residue, phenylalanine residue, tryptophan residue, methionine residue, serine residue, threonine residue, cysteine residue, glutamine residue, glycine residue, asparagine residue , A tyrosine residue, a lysine residue, an arginine residue, a histidine residue, an aspartic acid residue, and a glutamic acid residue (in the present specification, unless otherwise specified, amino acids Residue means L-amino acid residue).
[0013]
The collagen peptide that can be used in the present invention may be a collagenase immobilized on an immobilized carrier such as free or chitovar according to a generally known method, for example, a method described in JP-A-7-82299. Can be produced by a batch method, a column method or a combination of these methods, preferably by setting the reaction temperature to 40 to 45 ° C.
[0014]
A peptide having a unique amino acid sequence which can be produced in this manner, most preferably a collagen peptide rich in the collagen tripeptide in which the positive integer n is 1 (in order to exert the intended effect of the present invention) It is preferable that the positive integer n is as small as possible). In particular, the antigenicity specific to gelatin or collagen is remarkably suppressed. And the like, can be further improved.
[0015]
In addition, the collagen peptide containing a peptide having a unique amino acid sequence has a positive integer n of 1 to 7, that is, an average molecular weight of 280 to 3000. It is preferable in that it can eliminate the antigenicity derived from it and maintain the safety on the skin at a higher level. The minimum value 280 of the average molecular weight is a molecular weight assuming the above-mentioned tripeptide in which the positive integer n is 1, and as described above, it is preferable to take a value as small as possible around 280.
[0016]
Furthermore, the content of the collagen tripeptide having a unique amino acid sequence (where the positive integer n is 1) in the collagen peptide is preferably 25 to 100% based on the collagen peptide.
[0017]
As described above, the fractionation of a degraded product having a specific average molecular weight of a collagen peptide or the degraded product having a specific range of collagen tripeptide having a specific amino acid sequence (where the positive integer n is 1) is usually performed. A known fractionation method, for example, a gel filtration method, a reverse phase chromatography method, or the like can be used alone or in combination in some cases.
[0018]
Collagen peptide can be produced according to the above-mentioned method and contained in the product of the present invention, but a commercially available product such as collagen tripeptide series (manufactured by Miyagi Chemical Industry Co., Ltd.) can also be used. It is possible.
[0019]
The collagen peptide in the present invention can be contained as it is in skin external preparations or foods and drinks, but its content is slightly different depending on the type of applied dosage form, the method of use, and the expected degree of action. 0.001% or more, and particularly preferably 0.01 to 20.0%.
[0020]
If the content is less than 0.001% based on the whole composition, it is difficult to sufficiently exert the hyaluronic acid production promoting action, which is not preferable. Conversely, the content exceeds 20.0%. Even so, the tendency for the enhancement of the above effects to be inadequate in proportion to the increase in the content increases, which is not only unfavorable, but also may cause problems in the preparation, such as safety.
[0021]
In addition, the hyaluronic acid production promoter or skin external preparation of the present invention, the dosage form and form of the food and drink are arbitrary, such as capsules, powders, granules, solids, liquids, gels, bubbles, emulsions, creams, ointments, sheets, etc. It can be used in the form of a mixture.
[0022]
The field of use can be used, for example, in all kinds of internal / external preparations (including preparations used for animals), and specifically, ampules, capsules, pills, tablets, powders, granules, solids, liquids 1) pharmaceuticals, 2) quasi-drugs, 3) topical or systemic skin cosmetics (for example, lotion) , Emulsions, creams, ointments, lotions, oils, packs, and other basic cosmetics, facial cleansers, skin cleansers, massage agents, cleansing agents, hair removers, depilatory agents, shaving treatments, after-shave lotions, pre-shaves Makeup cosmetics such as lotions, shaving creams, foundations, lipsticks, blushers, eye shadows, eyeliners, mascaras, perfumes, nail polish, nail enamel, Nail enamel removers, cataplasms, plasters, tapes, sheets, patches, aerosols, etc.), 4) medicinal and / or cosmetic preparations applied to the scalp / hair (eg, shampoos, rinses) , Hair treatment agents, pre-hair treatment agents, permanent liquids, hair dyes, hair styling agents, hair tonics, hair growth and hair restoration, cataplasms, plasters, tapes, sheets, patches, aerosols, etc.) 5) Bathing agents to be poured into bath water, 6) In addition, axillary deodorants, deodorants, deodorants, antiperspirants, hygiene products, sanitary cotton, wet tissues, toothpastes, mouth fresheners, mouthwashes, etc. No.
[0023]
Further, as foods and drinks, oral compositions (gum, candy, etc.), processed fishery products such as kamaboko, chikuwa, livestock products such as sausage, ham, Western confectionery, Japanese confectionery, raw noodles, Chinese noodles, boiled noodles, buckwheat, etc. Noodles, sauces, soy sauce, sauces, sugar, honey, seasonings such as powdered candy, starch syrup, spices such as curry powder, mustard powder, pepper powder, jam, marmalade, chocolate spread, pickles, soy sauce, sprinkles, or Processed vegetables and fruits such as canned and bottled vegetables and fruits, dairy products such as cheese, butter, yogurt, miso soup, soups, fruit juices, vegetable juices, whey drinks, soft drinks, beverages such as alcoholic beverages, etc. It can be used for general foods and drinks such as health foods and health supplements.
[0024]
In addition, the hyaluronic acid production promoter or the external preparation for skin of the present invention, the food and drink, if necessary, in addition to the above-mentioned essential components, optional components and additives exemplified below within a range that does not impair the effects of the present invention. The content in the preparation is not particularly limited, but is usually preferably 0.0001 to 50%.
[0025]
(1) Various fats and oils
Avocado oil, almond oil, fennel oil, sesame oil, olive oil, orange oil, orange luffer oil, sesame oil, cocoa butter, chamomile oil, carrot oil, cucumber oil, tallow fatty acid, kukui nut oil, safflower oil, shea butter, liquid shea Fat, soybean oil, camellia oil, corn oil, rapeseed oil, persic oil, castor oil, cottonseed oil, peanut oil, turtle oil, mink oil, egg yolk oil, palm oil, palm kernel oil, mocro, coconut oil, tallow, lard , Squalene, squalane, pristane, hydrogenated products of these oils and fats (hardened oils, etc.) and the like.
[0026]
(2) waxes
Beeswax, carnauba wax, whale wax, lanolin, liquid lanolin, reduced lanolin, hard lanolin, candelilla wax, montan wax, shellac wax, rice wax and the like.
[0027]
(3) Mineral oil
Liquid paraffin, vaseline, paraffin, ozokeride, ceresin, microcrystalline wax and the like.
[0028]
(4) Fatty acids
Lauric acid, myristic acid, palmitic acid, stearic acid, behenic acid, oleic acid, linoleic acid, linolenic acid, docosahexaenoic acid, eicosapentaenoic acid, 12-hydroxystearic acid, undecylenic acid, tall oil, natural fatty acids such as lanolin fatty acids, Synthetic fatty acids such as isononanoic acid, caproic acid, 2-ethylbutanoic acid, isopentanoic acid, 2-methylpentanoic acid, 2-ethylhexanoic acid, and isopentanoic acid.
[0029]
(5) alcohols
Natural alcohols such as ethanol, isopyropanol, lauryl alcohol, cetanol, stearyl alcohol, oleyl alcohol, lanolin alcohol, cholesterol, phytosterol, and phenoxyethanol; and synthetic alcohols such as 2-hexyldecanol, isostearyl alcohol, and 2-octyldodecanol.
[0030]
(6) Polyhydric alcohols
Ethylene oxide, ethylene glycol, diethylene glycol, triethylene glycol, ethylene glycol monoethyl ether, ethylene glycol monobutyl ether, diethylene glycol monomethyl ether, diethylene glycol monoethyl ether, polyethylene glycol, propylene oxide, propylene glycol, polypropylene glycol, 1,3-butylene glycol , Pentyl glycol, glycerin, pentaerythritol, threitol, arabitol, xylitol, ribitol, galactitol, sorbitol, mannitol, lactitol, maltitol and the like.
[0031]
(7) Esters
Isopropyl myristate, isopropyl palmitate, butyl stearate, hexyl laurate, myristyl myristate, oleyl oleate, decyl oleate, octyl dodecyl myristate, hexyldecyl dimethyloctanoate, cetyl lactate, myristyl lactate, diethyl phthalate, phthalate, phthalate Dibutyl acid, lanolin acetate, ethylene glycol monostearate, propylene glycol monostearate, propylene glycol dioleate and the like.
[0032]
(8) Metal soaps
Aluminum stearate, magnesium stearate, zinc stearate, calcium stearate, zinc palmitate, magnesium myristate, zinc laurate, zinc undecylenate and the like.
[0033]
(9) Gum, sugar or water-soluble polymer compound
Gum arabic, benzoin gum, dammar gum, guaiac oil, Irish moss, karaya gum, tragacanth gum, carob gum, quinseed, agar, casein, lactose, fructose, fructose, sucrose or esters thereof, trehalose or derivatives thereof, dextrin, gelatin, pectin, starch, Carrageenan, carboxymethyl chitin or chitosan, hydroxyalkyl (C2-C4) chitin or chitosan, alkylene (C2-C4) oxide, such as ethylene oxide, low molecular weight chitin or chitosan, chitosan salt, sulfated chitin or chitosan, phosphorus Oxidized chitin or chitosan, alginic acid or a salt thereof, hyaluronic acid or a salt thereof, chondroitin sulfate or a salt thereof, heparin, ethyl cellulose, methyl cellulose, carboxymethyl cellulose, potassium Boxyethyl cellulose, sodium carboxyethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, nitrocellulose, crystalline cellulose, polyvinyl alcohol, polyvinyl methyl ether, polyvinyl pyrrolidone, polyvinyl methacrylate, polyacrylate, polyalkylene oxide such as polyethylene oxide and polypropylene oxide Or a crosslinked polymer thereof, a carboxyvinyl polymer, polyethyleneimine and the like.
[0034]
(10) Surfactant
Anionic surfactants (alkyl carboxylate, alkyl sulfonate, alkyl sulfate, alkyl phosphate), cationic surfactants (alkyl amine salt, alkyl quaternary ammonium salt), amphoteric surfactant: carboxylic acid Type amphoteric surfactant (amino type, betaine type), sulfate ester type amphoteric surfactant, sulfonic acid type amphoteric surfactant, phosphate ester type amphoteric surfactant, nonionic surfactant (ether type nonionic surfactant) Agent, ether ester type nonionic surfactant, ester type nonionic surfactant, block polymer type nonionic surfactant, nitrogen-containing nonionic surfactant), other surfactants (natural surfactant, protein) Derivatives of hydrolysates, polymeric surfactants, surfactants containing titanium and silicon, fluorocarbon surfactants Etc..
[0035]
(11) Various vitamins
Vitamin A group: retinol, retinal (vitamin A1), dehydroretinal (vitamin A2), carotene, lycopene (provitamin A), vitamin B group: thiamine hydrochloride, thiamine sulfate (vitamin B1), riboflavin (vitamin B2), Pyridoxine (vitamin B6), cyanocobalamin (vitamin B12), folic acids, nicotinic acids, pantothenic acids, biotins, choline, inositols, vitamin C group: vitamin C acid or derivatives thereof, vitamin D group: ergocalciferol (vitamin D2 ), Cholecalciferol (vitamin D3), dihydrotaxerol, vitamin E group: vitamin E or its derivatives, ubiquinones, vitamin K group: phytonadione (vitamin K1), menaquinone (vitamin K2), menadione (vitamin 3), menadiol (vitamin K4), other essential fatty acids (vitamin F), carnitine, ferulic acid, .gamma.-oryzanol, orotic acid, vitamin P such (rutin, eriocitrin, hesperidin), vitamin U and the like.
[0036]
(12) Various amino acids
Valine, leucine, isoleucine, threonine, methionine, phenylalanine, tryptophan, lysine, glycine, alanine, asparagine, glutamine, serine, cysteine, cystine, tyrosine, proline, hydroxyproline, aspartic acid, glutamic acid, hydroxylysine, arginine, ornithine, histidine And their amino acids such as sulfates, phosphates, nitrates, citrates, and pyrrolidone carboxylic acids.
[0037]
(13) Various additives derived from plant or animal raw materials
These are processed in a conventional manner according to the type and form of the product to be added (eg, pulverization, milling, washing, hydrolysis, fermentation, purification, squeezing, extraction, fractionation, filtration, drying, pulverization). , Granulation, dissolution, sterilization, pH adjustment, deodorization, decolorization, etc., are arbitrarily selected and combined), and arbitrarily selected from various materials.
[0038]
The solvent used in the extraction may be selected in consideration of the purpose and type of the product to be provided, or the processing to be performed later, but is usually selected from water, methanol, ethanol, propyl alcohol, isopropyl alcohol, and butanol. , One selected from lower alcohols such as isobutanol or water-containing lower alcohols, polyhydric alcohols such as propylene glycol, 1,3-butylene glycol, glycerin or water-containing polyhydric alcohols, acetone and ethyl acetate. Alternatively, it is desirable to use a mixture of two or more. However, when the content of the organic solvent is not preferable depending on the application, only water may be used, or ethanol which is easily removed after extraction may be used, and may be used alone or in an arbitrary mixed solution with water. Exploited extract may be used.
[0039]
In addition, when an additive derived from a plant or animal-based material is applied to a systemic or topical external preparation or cosmetics, the skin and hair are protected, the moisturizer is improved, the feel / feel is improved, flexibility is imparted, and stimulation is provided. Mitigation, alleviation of stress by aroma, cell activation (prevention of cell aging), suppression of inflammation, improvement of skin and hair quality, prevention and improvement of rough skin, hair growth, hair growth, hair loss prevention, gloss imparting, cleaning effect, In addition to cosmetic effects such as alleviation of fatigue, promotion of blood flow, and warm bath effect, effects such as fragrance, deodorization, thickening, preservation, and buffering can be expected.
[0040]
In foods and drinks, nutritional supplementation, recovery from fatigue, tonicity, cell activation (prevention of cell aging) and other health and cosmetic effects, improvement of taste, color and aroma, gloss imparting, stabilization, thickening, It can be used for antiseptic purposes. Furthermore, in addition to the above, various cosmetic and pharmacological effects of each raw material known so far are expected, and by combining these, the intended effect can be enhanced or multifunctional. It is also possible to make a product that expects an effect.
[0041]
As a specific plant (crude) used as a raw material, for example, almond (amber peach), eye (aiba), blue moss (Seifu wisteria), blue oyster (Aoki), blue ash or Kenasia ogiri (Otogi), blue porphyra (Kimomi) , Aotema, acacia, red ginger (red maize), red crocodile "fruit", red clover, red grape, red akaminoki (logwood), red akameshiwa (red bud), red akamane or rape or madder (akane grass root), red pheasant or pheasant (land yellow), Agi (Awei), Aki larch, Akinire (Lolyu bark), Aguay Guacu, Aguahe (Omiten palm), Akebi (Kidori), Asa (Jinji), Malva Asagao or Asagao (Toshiboshi), Hydrangea (Hydrangea), Ashitaba (morning leaves), Azuki (red azuki bean), Asunaro, Acerola, Acene Ku (Asenyaku), Achilla (Shokuyoukanna), Anise, Abiu, Abiurana, Brassica, Abemaki "fruit", Avocado, Ama, Amacha (sweet tea), Amachaworu, Amadokoro (Tamatake), Amana (Kojingi), Amaranth (Amaranthus, Balsam pear, Hymenoptera, Senninkoku, Sugimorigeito, Hosoaoigato, Aogaito, Haribiyu, Aosuzuran, Amaranthus hippochondriax), Amigasa lily or Bimo (shellfish), Aristolomelia (Yurizisen), Arcana (Alcanabo), Alcana , Altea, Arnica, Alpinia or Katsumadai (Sou-zuku), Angelica, Apricot or Hong-anzu (Anjin), Ansokkou (benzoic), Yellow Sapote, Ikakozolina (Biliary Head), Epimedium or Yasuyamatai Risou (inyokaku), rush (light heart grass), knotweed (tiger cane root), yew (first place), strawberry, fig (fructose "fruits and leaves"), strawberry (first), ichibi (winter aoiko), ichiyakuso (one) Medicinal herbs), Ginkgo (Ginkgo "seed, leaf"), Itohimehagi (Enshi), carob, carob bean, Inouzuna (Teilexi), Inubeyu (Honagainubiyu), rice "seed, seed coat", Inondo "seed," Ibuki-kusou, nettle, ylang-ylang, Iwatobacco (Iwasekichi), Iwahiba or Iwamatsu (makikashi), kidney beans, oolong tea, fennel (fenka), wheat (chibusanoki), ukiyagara (sanryou), Uguisukagura "fruit", Himeuikyo, turmeric (Usukin), duckweed ( Ubu-Pai), Usubasaishin or Keirinsaishin or Shoushusaishin (spicy), Usbe Nyaoi, Utsubenitachioi, Uchoura, Utsubogusa (summer hay), Udo or Shishido (Qanggai, Independent, Tangdokuhi), Unyadegato (Unkaria, Cat's Claw), Uba tea, Ume (Umeume "seed, pulp"), Uragirogashi, Uwaurushi (Kusaco peach), Unshuang mandarin orange (Chen skin), Unboku, Estragon, Ezocogi (Ezo Goka), Ezo lily of the valley, Echinashi (Hosobamurasakibarengiku), Enishida, Enokitake (Enotake mushroom), Ebisugusa or Cassia tora (Keiko), Elkanpreer, Berry "Fruit", Elemi, Eryngii or Pre-Lotus Eryngi, Endu (Gossip, Gossip rice), Pea, Forage or Kibana-ohgi (Astragalus), Narco lily or Kagirumabanarukoyuri (Yoshisei), Ohisasanoyuki or Sasanoyuki, Ouren (Yellow) ), Ogata Hawken, Ookarasuri (Karokon), Ooguruma (Public incense), Ootsu-la-Fuji (self-defense), Oobako (car front child, car front grass), Obanana thistle (Qizhou leash reed, bald head old man), Touashibami (haruko), toucanashi cactus , Obanana okera or Okera (white jujube), Obananasasuberi (banaba), Obanayasabushi "fruit", Oophosigusa (grain sperm), Omiatecatetsu, Omihasashi or Hawthorn (mountain versicolor), Oomiyashi, Barley (barley), Oka, Okaze Flooring), oguruma (turning over), okururihigotai (yushu sashimi, indigo head), panax ginseng or horseshoe ginseng (carrot), hypericum or lycopodium perforatum or perforated periwinkle (brother's cut grass), lycopodium (interrupted), onamiomi (blue) Ears), Onigurumi, Onidokoro or Koro or Nagadokoro (Hikai), Oninoyagara (Tianma), Onyuri or Sasasayuri or Hakatayuri (Yuri), Onoelan, Ononis, Halibut (Hibaha), Ominaeshi (Loose), Oyuco, Dutch mustard (Cresson), Dutch jelly, Dutch biyu , Dutch honeysuckle, olives "fruit, seeds, leaves", oregano, orange "fruits, peels", calyxio (ripe yellow), cacao "fruits, peels, seeds", oysters (persimmon "leaves"), kagikazura (fishing) Fuji hook), Persimmon or Kantriso (Rensenso), Kakiran, Gaku hydrangea, Cassia, Cinnamon (Kozomi "fruit"), Kajutsu (Shazajutsu), Kashiwa (Yamaki, Yuzuha), Kascarilla, Kascara sagrada, Kasumiso , Narcissus, kanikusa (Kin Sato), kaniwa, carnation, valerian (Kikusone), hippo, mosquito Noble or weeping birch (white birch), gerbera, pumpkin, kapok noki "seed", kahoku sansho (shopper), gama (bamboo), chamomile or roman chamomile, chamomile (desert plant), cam cam (camo duck), karagwa or yamaguwa (mulberry straw) , Color, karaksakeman, crow squirrel or cinnamon squirrel (flower melon), crow squirrel (midsummer), mussels, guarana "seed", karahoo (poor), karaya, garyu, karin (mochi), garcinia, kawamidori, kawara saiko Rape, vermilion grass), Kawazusha, Kawaratake, Kawaranadeshiko (stone bamboo) or Ezokawaranadeshiko (Qumugi, Quumiko), Kawarangin (blue ginseng), Japanese sagebrush (Inchinko), Kansui (sweet), licorice (licorice), Kantala Asa, Candelilla, Gantau, Canna, Kanglan, Kiichi Goose (Ezo strawberry, Dutch strawberry, Shrimp strawberry, Sawberry strawberry, Maple strawberry, European strawberry), Kiwi "fruit, leaf", Kikarasuuri (Urorone), Kikyo (Kikyo, Kikyo root), Kiku (Chrysanthemum, Chrysanthemum, Datura) Kikutaniki, Catalpa (Azumitsu), Rigid marble (Yotei), Pheasant (Kikomi), Kizuta, Kidachi aloe, Kachichihakka, Kina, Kinoki (Shincona, Akakinoki), Quinua (Kinoa), Kida (Huangbashi), Kimame, Gimnema sylvesta, Kimmenkaku, cabbage, cabbage "immature fruit", caraway, cucumber, gyoryu (Nishikawayanagi, Ichiyanagi), Kiraja saponaria, Kiraya, Kiranso (gold pea grass), Kumquat "fruit", Kinkiju, Kinsekai, Kimbusen, Kinma, Gold Finch Orchid, ginran, kinryohen, guapeva vermelilla, guava "fruit", guayule, quelux infectria (gallicate), cucuinuts, cugenumaran, wolfberry (cucumber, cucumber, cucumber leaf, ground skin), cucumber (tenmen winter), Kudzu (Kakkon), camphor tree, gooseberry "fruit", ginseng (yellow flower), cutie river, gardenia (garden garden), kunugi (bokusou), cupuas, kubeba, kumazasa, kuromatsuzura (horse whip grass), clara (bitter) ), Cranberry “fruit”, chestnut “seed, fruit, astringent skin”, curculigo lachifolia “fruit”, grapefruit “fruit / leaf”, buckthorn, black grey-mochi (needs rescue), turtle bamboo stalk or black banana stalk or cypress (extended life grass), Cloves (cloves, cloves), Gumbina's (Sekimei, Sekimeishi), Kaigai (Echigo, Ikugoho), Celosia (cow flower, Chicory), Gekkavidin, Gekkeiju (laurel), Kenashisarutoribara (Tokubukuryo, Yamakiko), Gentian, Gennoshoko (Older grass), Kemponashi ( Kigushi), Koukisekikoku, Kishumitsukan (Koji, Tachibana, Obenimikan, Hukuremican, Sagamikouji, Ponkan, Santara (Tachibana bark)), Koushinbara (Monthly flower), Kouzihakka, Kouzo "fruit", Kocha (tea), Kouhone ( River bones), Kouhon (straw and Kara straw), Kouriang, Kourikyo (Korakyo), Cilantro “fruit”, Kourouen (huhuanglian), Koganebana (yellow gon), Cowberry (Koshibachi), Coco palm “fruit”, Goshyu ( Goshuyu), goshoichigo (covered bonko), pepper (pepper), kos Moss, copaiba balsam, coffee "seed, leaves", kobushi or magnolia (hot spring), burdock (burdock, burdock), kobotdur, sesame (sesame), sesame hawakusa (gensin), beetle (gomiko), scallop or binankazura or matsubusa , Wheat (wheat), rice or rice bran "red bran, white bran", rice oil, cola acuminata "seed", cola vera "seed", Coleus forskoli, colony llah, fenugreek "fruit", colombo, conzulango, kelp, Konjac, Comfrey (Finari grass), Sisal (Sisal Asa), Saihylan, Sakaneran (Ezosakaneran), Sakisima button or Sinako button or Sesame ninsou (Eisei sensin), Sakura (Oshiyamazakura, Yamazakura, Oyamazakura, Edhigashi, Mayamazakura, Miyazakura) , Yoshino Somei Takanezakura, Kasumizakura, Kohigan, Satozakura, Kanzakura "leaves, flowers, fruits, bark (cherry bark)"), cherries, pomegranate, sasa, sasabaginran, sasanqua, sagemodaka (sawashatsu), sweet potato, sugarcane, sugar beet, saccharomyces ), Saffron (red safflower, western safflower), sapodilla, sapotea marillo, pomelo "fruit", saponso, salmon berry "fruit", sarashinasho (masuma), saransitou (sand indigo sashimi), salvia (sage), sawagikyo ( Sancho-na), Sawagurumi (Yamawomo), Sankaku-cactus, Sangre-degrado (Croton), Sansouyou-so, Sanshin-jinjin-jin (Seven carrots), Sanshuyu (sanshuyu), Sansho (sansho), Sandzukon (Yamane root) , Shea (carite), cyanoki "fruit", shiitake mushroom ( Mushrooms), Shion (Shien), Deer bean, Dickis, Shikunshi (Kimiko), Perilla or Aodiso or Chirimenji or Katamendiso (Shiyo-leaf, Shiso), Rosewood, Shinanoki, Sinahoonoki, Shinarengyou (Linus edulis), Shimejime , Hatake shimeji, Oshiroishi shimeji, Bunashimeji, Honjimeshi, Shirotamogitake), Shimokren (Spicy), Shimotsukeisou, Potato, Peonies (Shakuyaku), Shajin (Sansan), Jasmine (Matsurika), Janohige (Motomon winter), Jurokusage Gypsophila, gypsophila (sand sand, shrinking sand), juzdama, palm "fruit", shunran, ginger palm, ginger (ginger), jasper hydrangea (Joyama), shobu (iris, iris root), shozuku "fruit", Shirakashi "seed", silane (Biancucumber), Shirogou butterfly “seed”, white clover (clover), white adzuki (chicken bone grass), white rhinoceros (small iris), white rhododendron “flower”, white lunar lupine, white minant (minamitenmi), cinchona sax silbra, jinchoge , Mizuka flower, Shencho flower), Cinnamon, Cinnammum cassia (cinnamon bark), watermelon (watermelon), honeysuckle (gold and silver flower, Shinobi winter), sorrel (acid model), swimberry "fruit", star apple, stevia, strawberry "fruit", Suzu Psycho (Lord Xu), Horsetail (Thorn), Purslane (Horse Tongue, Hen Tonen), Plum "Fruit", Surugaran (Oran), Scots Pine "Koke", Larch, Larch Hawthorn, Acacia catechu, European Dandelion, European horse chestnut (malonnier), pear "fruit", Atlantic pear, elderflower, elder tree, European pear (juniper, juniper), yarrow (milfoil), balsam pear, or euphoria , Horseradish, rosewood (Iris), seaweed (Sakurasekoku, Kosekisekku, Obananasekoku, Okinawasekoku, Honsecekku, Kochoseksoku, Shikaseksekoku, Kibananosekoku) (dendrobium, stones), cedrone , Senega, seri, celery, senkyu (river kyu), senshinren (shinshinren), sendan, sennin cactus, senna "fruit, leaf", senninsou (great tate) , Assemblage (drug), Sengoku, Soka (grass), Elephant Chestnut, Soshinwabai, Buckwheat "seed", Soybean Potato, Broad bean, Rhubarb (Ohio), Radish, Soybean, Daidai (orange peel, Kikomi), Takasagorurigotai (Southeast) Indigo sashimi), Takasaburo (Dried lotus grass), Takatodai (Dai-gei), Takawarabi (Inu spine), Dark sweet cherry "fruit", Tachiaoi, Tachijakosou (thyme, Momoka), Tachidokoro (Hikai), Tachiyanagi, Tamarind " Seeds ”, onion, tamshiba (hot spring), taro tree“ fruit, leaf, root bark ”, tarayou (large leaf winter blue, one leaf tea, bitter tea, bitter tea), dahlia (tenjikubotan), tarui, tanukirimame, tanshiuchiwa, tanjin (Dansin), dandelion (Kabukohide) or white dandelion or moko dandelion, dammar, cherry " Fruits ”, Tigaya“ Fruits, roots, buds ”, Chikusetsu carrots (bamboo ginseng), chicory, chancapiedra (Kidachikomikansou), tulips, chocolate stays, Korean thistles (artichokes), Korean daisies (daihoku), Korean noodles ( Wuyi), Artemisia (Aiba), Choreimaitake (Irei), Chorogi, Tsukimisou, Tsukushikaneran, Tsukuritake (Mushroom), Tsuchiakebi, Camellia, Camellia, Tsumekusa (lacquer primrose), Tsuyukusa (Duckweed), Tsuruazuki (Red azuki bean), sword beetle, sword beetle, sssssssssssssss, ssssssssssssssss, sssssssssssssssssssssss, sssssssssssssssssssssssssssssssssssss.4, c1sssssssssssssssssssssssssssss1, e, sssssssss, Touga (toucan), Toukagika La, Capsicum (Hot Pepper), Touki (Toki), Tokinsenka (Marigold), Touokaika, Toubena (River Cut), Maize or Maize Hair (Nanban Hair), Toad Mizuki (Female Sadako), Torindo or Kosenrindo (Ryubile), Tokusa (pirate), Dokudami (jujutsu), Tokon (root sprout), Toshishi or Mamedaoshi or Porpoise, Eucommia (Tochu "bark, leaves, roots"), Ash (Qin bark), tomato, tragacanth, triacanthos Seeds ", eustoma, tormentilla, dronoki, troloi, tonka bean, Niseria berry" fruit ", potato or yam (herbal medicine), nagikadada (butcher bloom), naginata kouju, nazuna, rape, nata bean or sword bean (sword bean), Jujube, jujube (large jujube), nannywaiva (Kanesakurako), Navena (intermittent), Nameko, Peanut (peanut), Nanten (Namitenmi), Namban-karamushi (ramie), Niosui-mire, Nigaki (Maki), Nigamogi (bitter), Nikuzuku, Kei or Nikkei Or Ceylon Nikkei or Yab Nikkei (cinnamon bark) or Keishi (Keishi), Ginkgo Butterfly, Chinese chive (Nirako), Elderberry (bone-bearing tree "fruit, flower, stem, leaf"), garlic (garlic), nurde (fivefold child), leek, Nemunoki or Nebu or Nebi or Nemurinoki or Jigokubana (Happiness), Nozami (Daichi), Noibara (Fruit), Nori Kazura (Ryoshohana), Nokoto, Yarrow, Nodatake (Maehu), Nobara, Nomomo, Pineapple "Fruit", Hibiscus ( Boussouge, fusulin boussouge, roselle), high gentian, hauchiwa, pau doce, hakamau Labosi (Bone Crushing), Hakuka Yumato, Hakusanchidori, Hakusen (Rinosaur), Huckleberry "Fruit", Hakobe (Fruit), Hazel (Haruko), Hashiridekoro (Roto Root), Basil, Lotus (Lotus, Lotus, Lotus) , Parsley (Netherlands jelly), naked mussels, batata, bees or medakes (bamboo-boiled), patchouli, mint (slightly loaded, thinly loaded leaves), hashi bean, pearl barley (yokuninin), hanasuge (chimbo), banana, hanaminta, hanabishi (satsurishi, (Shisitsuri), vanilla bins, papaya, chickweed (rat malt grass), pahalobobo, hub "whole plant, stem, leaf", paprika, hamadakiran, hamagou or honeysuckle (crop), hamasuge (katsukishi), hamabishi ), Hamanas (maikai flower), Hamabofu (beach storm), Hamamelis, palm, rose (rose), Lata, balata rubber tree, agaric mushroom (aratake mushroom, white agaric mushroom, usukimori no kasa), haran, pariurus (Prunus avium), parietalia, harkanran, balsamina (tsurureishi, bitter gourd), harunire (yu bark, yu bark, yuba), barbasco, haru Gentian, hand daikai (beach large sea), breadfruit, giant forsythia (sun dried), lycopodium carrot (party), pecan nut, giganbana (stone, garland), higuru madaria, higotai, hishi (hishimi), pistachio, beet, Hitotsuba (stone reed), Aedes aegypti, daisies (daisy), hinata nokozuchi (cow knees), hinata bulbul, hinoki, hinoki, castor, sunflower, bell pepper, himeuzu (heavenly aoi), hinimama (fragrance), himematsutake (kawariharatake, hiromatsutake) ), Himemuyo Orchid, Pimenta "Fruit", Juniper, Bianca cucumber, Hydrangea "Fruit", Chickpea, lentil, Below oyster, Hiroha okinagusa (Bakuoka), Loquat "Fruit, Leaf, Stem", Betel nut (Large clothes skin, Betel palm), Pu'er tea , Sperm Kazura (Southern Rattan), Butterflies, Butterbur Dandelion (Winter Flowers, Wheat Leaves), Fuji Bacama (Orchid Plants), Fuji Bean (Flax Beans), Fujimodoki (Costume Cherry, Seed Flowers), Grape "Fruits, Peel, Seeds, Leaves", Beech, Yumusinatsukutake (Cordyceps), Brazilian Licorice, Brazilian Carrot, Brazilian Palm, Brazilian Elephant Palm, Black Currant "Fruit", Blackberry, Bragnien, Plum "Fruit", Flucellaria, Blueberry (Prunus avium), Prune, Floral Blanca, blonde psyllium, bundou (mung bean), haese Lunut, Loofah, Hetkaran, Safflower (Safflower), Henneken, Belladonna, Berry "Fruit", Persea, Perbianberg, Pereschia grandifolia, Benquisou or duckweed (Keiten), Boysenberry "Fruit", Butterfly or Elderweed or Nendowe or Hawakigi Kokia (Skin), Hosairan, Balsam (Hosen, acute child, osteophyte), Potteria sapota, Potteria lukma, Spinach, Spinach, Physalis (Torone), Storm (Wakoboku, Park), Bokeh (Gourd Gourd), Hosoba Aburagiku (Bitter Yoku), Hosobaogoruma, Hosobanaokera (Sojutsu), Hosobanokirinsou (Kageten Sanshichi), Hosobaengengiku, Bodaiju (Bodaiki), Button (peony "flower, leaf, stem, bark") , Buttonpi (peony skin), hop, jojoba, borige (rurichi) ), Bordeaux, Whiteberry berry “fruit”, Hong Onik (Nikujyu Ryo, Daigei), Hongsekok (Iron skin stone, Sparrow mountain stone), Myzuruten Nansha (Tenan star), Maitake (Maitake), Maou (Maoh) ), Maca, Macadamia nuts, Margaret (Mokshungik), Macri (Seaweed), Magwa (Mulberry white bark "bark, leaves"), Magnolia splengeri, Masarampuba, Masaranzuba de Ceara, Masaira de Boi, Masahua (Tamanousenharen, Kyukonkinrenka), Matabi (Kinten Tate), Matsukasa, Pine “leaves, bark, roots”, Matta Oryo, Matsuhodo (Bukuryo), Matico (Cordon Sillo), Mayorum (Hanahakka), Malvanozadine ( Bittersweet), mulberry "fruit", quince, malein (below mousse), mango Mangosteen, Mansagna (Andesukamitsure), Mansugarumi, Mandarin "fruit", Mannentake (Lingzhi), Pheasant (Kikomi "fruit"), Mishimasaiko (Saiko), Mizuobabaco or Mizuasagao (Ryococcus), Mizokakushi (half-sided) Lotus), misohagi (Chikuna), beet willow or elder willow (edition), mitsugashiwa, honeywort, midori sapote, midorihakka, mimosa, myoga, miracle fruit "fruit", myrrh, myrobaran, mugiwaliku, mukuge, kokunoki (Prolonged skin), munya, purple (purple root), purple or purple purple (pearl), purple maize, purple purple (Kunming chicken blood wisteria), megakanza opuntica, mehajiki (mokugi grass), mebogi, Mematsuyoigusa (night primrose), Melaleuca, Meri Sassa, melilot, melon "fruit", moko mugwort, moso bamboo, mok giraffe, moje (koboku), mochinoki, moko (wood incense), maple blossom, peach (peach "leaves, seeds, flowers, fruits"), bean sprouts, Morero cherry "fruit", moroheiya (burlap), yakawamurasakiimo, yakuchi (masuchi), cornflower (cornflower), cornflower mint, yacon, yaba santa, yashabushi (arrow wheel) or himeyashabushi or himeyashabushi "fruit, peel, fruithead" , Willow willow, yatsude (octagonal gold plate), mistletoe (willow parasitism), willow (kawayanagi, tachiyanagi, weeping willow, akameyanagi, pussy willow, dog puppy willow, kinuyanagi, koryanagi, unryuyanagi, yamayanagi, oyster, oyster, oyster Willows and stalks, Willow stalks “leaves and stems”, scabbard, spar beetle (purple gold beef), squirrel tobacco (crane squirrels, Tenmeisei), pokeweed (commercial land), yamahanoki (mountain Haru), yamamomo (yang plum skin), Eucalyptus, Saxifraga (tiger eargrass), Yucca or flevifolia, Yuzu "fruit", lily, yorugusa, mugwort (Aiba), ligan (Rainuma), lime "fruit", rye, lacanca "fruit", raspberry "leaf, fruit" ”, Latania (Clameria), Rakkyo or Shallot (scallop), lavender, longan (longan meat), agave (Aono agave, fukurin agave), ryocha (green tea), apple“ fruit, seeds, leaves, roots ”, gentian, rubus or Suabisimus (sweeten), Ruritama thistle (Urajirohigotai, Xinjiang indigo head) , Litchi (Lichi, lichi nucleus), lettuce (chisha), red currant "fruit", red pitaya, lemon "fruit", lemongrass, forsythia or Chinese forsythia (forsythia), astragalus, waxby, wax palm, rocon ( Reeds, Loganberry "fruit", rosemary (mannen wax), rose hips (nobara), wasabi, cottonweed azalea (Mengmenhua), waremokou (jiyu) and the like.
[0042]
Examples of seaweeds include seaweeds [green algae: Chlorella vulgaris, Chlorella pyrenoidosa, Chlorella ellipsoidia, Aonori (Usbaaonori, Sujiaonori, Hiraaonori, Bowaonori, Hosoedaaonori), and seaweed [Aonaga] brown algae [Aonasa] (Macomb, Resiliconbu, Hosomebu, Mitsuikonbu), Wakame, Hirome, Aookame, Giant Kelp (Macrostistis pilifera, Macrocystis integrifolia, Neocitys Luetokeana), Hijiki, Hibamata, Umiuchiwa, Usubaumiuchiwa, Kiribano Sea Prickly Pear, Akabumi Prickly Pear, Konau Prickly Pear, Okinawa Prickly Pear, Usuki Prickly Pear, Ecchi Prickly Pear], Seaweed [Red Algae: Hijirimen, Maxa (Tengusa), Hiracusa, Onixa, Obaku ) Kainori, Yaleus Banori, Kagius Banori, Sujius Banori, Hyus Banori, Akamomininori] and the like.
[0043]
In addition, other algae, for example, green algae (Chlamydomonas: Chlamydomonas, Akayukimo, Dunaliella: Dunaliella, Chlorococcus: Chlorococcus, Kwanomimo: Genus Kwanomimo, Volvox: Ohigemawari, Volvox, Palmera, Yotsumemo, Aomidro: Hizaori, Spirogyra, Papilio spp., Hibimidro spp .: Hibimidro spp. Echinacea spp .: Echinacea spp., Iwata spp .: Husaiwata, Sricogulata, Helaiwata, Crocita, Janemo, Mir spp .: Mill, Cromil, Sakibuchimil, Nagamil, Hira Le, Casanori: Casanoli, Jusmo: Futozuzumo, Tamazuzumo, Mizojusmo, Mikazukimo, Korekaete, Tsuzumimo, Kikkogusa: Kikkougusa, Hitoexa Genus: Hitougusa, Hirohanohitengusa, Utsutoegumo, Genus Sumiremo: Sumiremo, Hoshimidoro, Fushinashimidoro etc.).
[0044]
Blue-green algae (Genus L .: L., L., L .: Kawatake, Ishikurage, Hassai, Juremo, L. (spirulina): Spirulina, Trichodesmium (Aiakashio), etc.).
[0045]
Brown algae (Piraella spp .: Piraella spp., Spirogyra spp .: Namiami spp., Isogrape spp .: Isogrape, Isogawara spp .: Isogawara spp. , Genus Sanadagusa: Sanadagusa, Fukurinamiji, common genus: common rush, terrestrial genus: Ezoyahazu, Yahuzugusa, Uraboshiyahazu, genus Genus: jigamigususa, Umiuchiwa genus: Genus genus, genus genus, genus Genus : Nabarimo, Chasoumen: Motsuchachasomen, Matsumo: Matsumo, Nagamatsumo: Nagamatsumo, Okinawamozuku: Okinawamozuku, fake Tomozuku genus: Nisefumozuku, Futomozuku genus: Futomozuku, Ishimozuku genus: Ishimozuku, Kuromo genus: Chromo, Nisemozuku genus: Nisemozuku, Mozuku genus: Mozuku, Ishige genus: Ishige, Irolo, Ichimegasa genus: Calligraphy Umibos, Urushigusa genus: Urushigusa, Keulushigusa, Tobacco moss, Kombmodoki: Kombudoki, Hambamodoki genus: Havamodoki, Habanori genus: Habanori, Hiranohabanori genus: Habanori, Commonbukuro genus: Commonbukuro, Ezobukurono, Ezobukurono, Ezobukuronozo, genus Genus: genus: genus: kagomenori: kagomenori, muralidori: murachidori, shakusugusa: sharksugusa, iwahige: iwahige, yokojimanori: yokojimanori, Genus: Cyperus, Fennel: Fennel, Turmo: Turmo, Anana: Aname, Sujime: Sujime, Miss kelp: Miss kelp, Atsuba Miss kelp, kelp: Gatsugara kelp, Kakisima kelp, Oni kelp, Gohei sorghum, Gohei kelp, Gohei sorghum Genus Oleoptera, Trolocomb: Trolocomb, Antokume: Antokume, Swordfish: Swordfish, Tsurarame, Kurome, Asterina: Asterine, Nesulecomb: Nesirecomb, Kuroshiome: Kuroshiome, Nekoashikame: Acerium, Araumenu Genus: Ainu-Wakame, Chigaiso, Oniwakamezo-Ezoishige: Ezoishige, Yabanemoku: Yabanmoku, Rapamoku: Rapamoku, Jiromoku: Uganomok, Jo Lomoku, Hiemoku, Hondawara: Tamanamoku, Isomoku, Nagashimamoku, Akamoku, Shidamoku, Hondawara, Nejimoku, Narasamo, Mametara, Tatsukuri, Yasumatamoku, Umitoranoo, Oobamoku, Hashimomoku, Haikumokugi, Hawk Mokumoku , Genus genus: genus auricularia, genus bulumium: genus bulumium, genus kayamoris: kayamoris, etc.).
[0046]
Red algae (Coleoptera genus: genus oxenori, fenori no moss, Amanori genus: Asakusanori, Susabinori, Uppulinori, Oniamanori, Tasa, Filitasa, Benitasa, Rhodocorton genus: Milnoveni, Akebonomozuku, Agenidae, Genus: Sea elephant, Tsukumonori, Kamogashiranori, Benimozuku: Benimozuku, Hosobenimozuku, Kasamatsu: Casamatsu, Fusanori: Fusanori, Nisefusanori: Nisefusanori, Sodegarami: Sodegaramiragara, Gallaghera Genus: Tamaitadaki, swordfish: swordfish, swordfish, slimebrush: slimebrush, swordfish, oobusa, nambugu , Kohira, Yohira, Kinukusa, Hibiroudo: Hibiroudo, Himehibiroudo, Isomemudoki: Isomemudoki, Michigaesou: Michigaesu, Ryumonsou: Ryumonsou, Hera Ryumon, Nisekarekisuba, Nisekareqaba, Nisekarekubasa Genus Naminohana: Hosobanaminohana, Naminohana, Coralthrum Genus: Garagaramodoki, Shiogosagoromo Genus: Shiogosagoromo, Iwanokawa Genus: Etsukiiwanokawa, Kainokawa Genus: Kainokawa, Kaninote Genus: Kaninote, Kanonomi, Genus, Sagomo, Dessertica Cyanophora, Sakuranori, Nikumukade, Tambanori, Smooth, Fudarak, Malbahudarak, Isonohana: Nuracusa, Ronulaxa, Giant Coleoptera, Hirakintoki: Hirakintoki, Pleurotus genus: Matabou, Kintoki: Chabokintoki, Kintoki, Matsunori, Komenori, Tosakamatsu, Aimatsumatsu, Fushikintoki, Tsunomukade, Nagakinotoki, Suzumekito, Kakutoitto, Kakutoto Genus Nagaobane: Nagaobane, Funori: Hanafunori, Fukuronori, Makuhnori, Kalexigusa: Kalexigusa, Tosakamokuki: Hosobanotosakamodo, Hirohanotosakamoki, Yatsudegatatosakamoki, Kurotosakanotoka, Japanese moss Tosakamomodo, Nankaitosakamoki, Himetosakamoki, Kinuhada: Kinuhada, Ezotosaka: Ezotosaka, Tsukasanori: Enashikarimenia, Otsukasanori, Hana Tacarimenia, genus genus: hornworm, lycaenoid genus: lycopodium, cypress, genus genus: nikhonoo, venicenago genus: venicenago, sukakebeni genus: sukakebeni, okamurasa genus: yamadagusa, mirin genus: mirin, saucaminae spp. , Isomocca: Isomocca, Yukari: Yukari, Hosoyukari, Ibaranori: Ibaranori, Rhinoceros, Tachiibara, Kagiibaranori, Kizinoo: Kijinoo, Isosanthus: Isodantsuu, Atsubanori: Atsubanori, Ogononori, Ogonotori, Ogonotori, Mizo-Okonori, Kabanori, Beniogonori, Fukuroshinori, Murasaki Kabanori, Shinkaikabanori, Togekabanori, Kataogonori, Ryukyuogono Li, Sorbus americana, Cyperus sylvestris, Ligustrum serrata, Lily of the valley, Genus sylvestris, L. Genus: crocodile, saimi, crocodile, lotus spp .: lotus spp. Genus goatsaw: goatsaw, hanasakura, smeltfish: smelt, scorpionfish, hananoeda genus: hananoeda, hiratagoats: hiratagoat, darsu : Darus, masagoshibari, anadarus, webagsa genus: webugsa, Benifukuronori: Benifukuronori, Fushitsunagi: Fushitsunagi, Himefushinagi, Hirohafushinagi, Watsunagisou: Hirawagnagisou, Utsuwagisuigi, Iriomota, Iriomota , Genus Egonori: Egonori, Futogigis, Saeda genus: Saeda, Chrysanthemum genus: Chirimomiji, Konohanoriaceae: Habutaenori, Konohanori, Suzurononori, Usveni genus: Usveni, Genus: Hasuziginu, Nagakonosuginonu Genus: Kagiusbanori, Yaleusbanori, Siusiusbanori, Hyusbanori, Usubanorimodoki Genus: Usubanolimodoki, Ayanishiki genus: Ayani Ki, Ayaginu: Ayaginu, Dadia: Enashidazia, Shimadada: Isohagi, Shimadada, Dazimodoki: Dazimomodoki, Itogusa: Moletogusa, Futigusa, Makri: Makuri, Sanaginori: Hanayanagi, Yuna, Yanaginaginamo, Yanaginaginamomori, Yanaginaginomibe, Yanaginaginomibe, and Yanaginaginomibe, Yanaginaginomibe, Yanaginarinamoyana, Yanaginaginoyamo, Yanaginarinamoyana, Yanaginayori, Yanaginayorimo, Yanaginayori, and Yanaginayori-namo-na-yagi-namo-nagi-na-gori , Sasabayanaginori, Sozogenus: Kurosozo, Kobusozo, Hanesozo, Sozonohana, Hanegusa: Hanegusa, Kehanegusa, Kozanemo: Kozanemo, Isomurasaki, Hosokozanemo, Genus: Himegoke, Kurohoshigoshi, Hioudoogoshigu Isomegusa, Aspera spp .: Aspera spp., Aspium spp .: Aspium spp., Aspium spp .: Aspium spp., Sawtooth spp. : Kawamozuku, Aokawamozuku, Himekamozuku, Ideyukogome spp .: Ideyukogome, Okichimozuku: Ochichimozuku, Itogusa, Chinorimo: Chinorimo, Chisinori: etc.).
[0047]
Axle algae (Erythra spp., Echinacea spp., Hoshiturimo genus: Hoshiturimo, Lichnotamus genus, Phalaenopsis genus: Himejirabamo, Chaboflavidium, Tripella genus, etc.), yellow algae (Hikarimo genus: Hikarimo etc.) and the like.
[0048]
In addition, as a material derived from animal-based raw materials, a cockscomb extract, a cow or pig, a human placenta extract, a cow or pig stomach, a duodenum, an intestine, an extract of a spleen or a decomposition product thereof, a cow or pig brain tissue Extract, collagen hydrolyzate (acid, alkali, enzyme, etc.) hydrolyzate of bovine / pork or fish, collagen derivative such as water-soluble collagen or acylated collagen, elastin or elastin hydrolyzate of bovine / pork / fish (acid, Alkali or enzyme) or water-soluble elastin derivative, keratin and its degradation product or their derivative, silk protein and its degradation product or their derivative, pig or bovine blood cell protein degradation product (globin peptide), cow or pig hemoglobin Decomposition products (hemin, hematin, heme, protohem, heme iron, etc.), milk, casein and its decomposition products or derivatives thereof, skim milk powder and its decomposition products or it Derivatives, lactoferrin or decomposed product thereof, egg ingredients, fish hydrolyzate, nucleic acid-related substance (ribonucleic acid, deoxyribonucleic acid), or the like.
[0049]
In addition, as a material derived from a plant or animal material, any site, cell, tissue, organ, metabolite, or the like derived from a genetically modified product or a cell fusion can be used. Furthermore, for example, undifferentiated cells or cells undergoing differentiation, which are obtained by culturing cells at any site, cell, tissue, organ, or the like, can also be used.
[0050]
(14) Marine components
Seawater such as deep water, for example, seawater salt, dried seawater, inorganic salt obtained from the Dead Sea or the Atlantic or Pacific Ocean (sodium chloride, magnesium chloride, potassium chloride, etc.), sea mud or mud (fungo), for example , Italian fango, German fango, Eifelfango, Freiburgfango, etc. sea mud or mud (containing components: silicon dioxide, titanium dioxide, aluminum oxide, iron oxide, manganese oxide, sodium oxide, potassium oxide, magnesium oxide, calcium oxide , Strontium oxide, sodium, potassium, magnesium, calcium, chromium, iron, copper, nickel, zinc, lead, manganese, arsenic, water), saintokushi, black pearl stone, etc.
[0051]
(15) Microbial culture metabolite
Yeast metabolite, yeast extract, bacterial metabolite, bacterial extract, mold or actinomycete metabolite, mold or actinomycete extract, natto metabolite, natto extract, rice fermented extract, rice bran (red bran, white bran) ) Fermented extract, Euglena extract or its decomposition product or water-soluble derivative thereof, trehalose or its derivative, lactic acid fermentation product of raw milk or skim milk powder, lactic acid fermentation product of leguminous plant, lactic acid fermentation product of coconut genus plant, and the like.
[0052]
(16) α-hydroxy acids
Glycolic acid, citric acid, malic acid, tartaric acid, lactic acid, etc.
[0053]
(17) Inorganic pigment
Silicic anhydride, magnesium silicate, talc, kaolin, bentonite, mica, titanium mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, calcium carbonate, magnesium carbonate, yellow iron oxide, red iron oxide, black iron oxide , Gunjou, chromium oxide, chromium hydroxide, carbon black, calamine, etc.
[0054]
(18) UV absorber / blocker
Benzophenone derivatives (2-hydroxy-4-methoxybenzophenone, 2-hydroxy-4-methoxybenzophenone-5-sulfonic acid, sodium 2-hydroxy-4-methoxybenzophenone-5-sulfonate, dihydroxydimethoxybenzophenone, dihydroxydimethoxybenzophenone-sulfone Sodium acid, 2,4-dihydroxybenzophenone, tetrahydroxybenzophenone, etc.), paraaminobenzoic acid derivatives (paraaminobenzoic acid, ethyl paraaminobenzoate, glyceryl paraaminobenzoate, amyl paradimethylaminobenzoate, octyl paradimethylaminobenzoate, etc.) , Methoxycinnamic acid derivatives (ethyl paramethoxycinnamate, isopropyl paramethoxycinnamate, octyl paramethoxycinnamate, paramethoxycinnamate 2-ethoxyethyl, sodium paramethoxycinnamate, potassium paramethoxycinnamate, glyceryl mono-2-ethylhexanoate diparamethoxycinnamate, etc., salicylic acid derivatives (octyl salicylate, phenyl salicylate, homomenthyl salicylate, dipropylene glycol salicylate, ethylene glycol salicylate, Myristyl salicylate, methyl salicylate, etc.), anthranilic acid derivatives (methyl anthranilate, etc.), urocanic acid derivatives (urocanic acid, ethyl urocanate, etc.), coumarin derivatives, amino acid compounds, benzotriazole derivatives, tetrazole derivatives, imidazoline derivatives, pyrimidine derivatives , Dioxane derivative, camphor derivative, furan derivative, pyrone derivative, nucleic acid derivative, allantoin derivative, nicotinic acid derivative , Vitamin B6 derivatives, umbelliferone, esculin, benzyl cinnamate, sinoxate, oxybenzone, dioxybenzone, octabenzone, slisobenzone, benzoresorcinol, arbutin, guaiazulene, shikonin, baicalin, baicalein, berberine, neoheliopan, escalol, zinc oxide, Talc, kaolin, etc.
[0055]
(19) Whitening agent
Paraaminobenzoic acid derivatives, salicylic acid derivatives, anthranilic acid derivatives, coumarin derivatives, amino acid compounds, benzotriazole derivatives, tetrazole derivatives, imidazoline derivatives, pyrimidine derivatives, dioxane derivatives, camphor derivatives, furan derivatives, pyrone derivatives, nucleic acid derivatives, allantoin derivatives , Nicotinic acid derivatives, vitamin C or derivatives thereof (vitamin C phosphate magnesium salt, vitamin C glucoside, etc.), vitamin E or derivatives thereof, kojic acid or derivatives thereof, oxybenzone, benzophenone, arbutin, guaiazulene, shikonin, baicalin, baicalein , Berberine, placenta extract, ellagic acid, lucinol, etc.
[0056]
(20) Tyrosinase activity inhibitor
Vitamin C or its derivative (vitamin C phosphate magnesium salt, vitamin C glucoside, etc.), hydroquinone or its derivative (hydroquinone benzyl ether, etc.), kojic acid or its derivative, vitamin E or its derivative, N-acetyltyrosine or its derivative , Glutathione, hydrogen peroxide, zinc peroxide, placenta extract, ellagic acid, arbutin, rucinol, silk extract, plant extract (chamomile, mulberry, gardenia, touki, waremokou, clara, mugwort, honeysuckle, yellowfin, dokudami, matsuhodo, Adlay, Pompilot, Hops, Hawthorn, Eucalyptus, Yarrow, Altea, Calypt, Mankeishi, Hamamelis, Karaguawa or Yamaguwa, Life-extended grass, Bellflower, Toshishi, Japanese sponge, Sun dried, Mao, Sengoku, Dokkatsu, Psycho, Ufuu, littoralis, Scutellaria, peony bark, peony, cranesbill, kudzu root, licorice, Fushi aloe, Cimicifuga, safflower, green tea, black tea, Ali elixir), and the like.
[0057]
(21) Melanin pigment reduction / decomposition substance
Phenylmercury hexachlorophene, mercuric oxide, mercurous chloride, aqueous hydrogen peroxide, zinc peroxide, hydroquinone or its derivatives (hydroquinone benzyl ether) and the like.
[0058]
(22) Turnover promoting action / cell activator
Hydroquinone, lactic acid bacterium extract, placenta extract, reishi extract, vitamin A, vitamin E, allantoin, spleen extract, thymus extract, yeast extract, fermented milk extract, plant extract (aloe, ogon, horsetail, gentian, burdock, sicon, carrot, Hamamelis, hops, okinin, edulis, assembly, touki, balsam, amacha, hypericum, cucumber, scotch, mannenrou, parsley) and the like.
[0059]
(23) Astringent
Succinic acid, allantoin, zinc chloride, zinc sulfate, zinc oxide, calamine, zinc paraphenol sulfonate, potassium aluminum sulfate, resorcinol, ferric chloride, tannic acid (including catechin compounds) and the like.
[0060]
(24) Active oxygen scavenger
SOD, catalase, glutathione peroxidase and the like.
[0061]
(25) Antioxidant
Vitamin C or a salt thereof, stearic acid ester, vitamin E or a derivative thereof, nordihydroguaseletenic acid, butylhydroxytoluene (BHT), butylhydroxyanisole (BHA), hydroxytyrosol, parahydroxyanisole, propyl gallate, sesamol , Sesamolin, gossypol, propolis etc.
[0062]
(26) Lipid peroxide production inhibitor
β-carotene, plant extracts (sesame cultured cells, amacha, Hypericum perforatum, hamamelis, clove, melissa, emisoso, birch, salvia, mannenrou, Nantenmi, Eijitsu, Ginkgo, green tea) and the like.
[0063]
(27) Anti-inflammatory agent
Icutamol, indomethacin, kaolin, salicylic acid, sodium salicylate, methyl salicylate, acetylsalicylic acid, diphenhydramine hydrochloride, d-camphor, dl-camphor, hydrocortisone, guaiazulene, camazulene, chlorpheniramine maleate, glycyrrhizic acid or a salt thereof, glycyrrhetinic acid or a salt thereof Salt, licorice extract, sicon extract, age extract, propolis and the like.
[0064]
(28) Antibacterial, disinfectant, disinfectant
Acrinol, sulfur, calcium gluconate, chlorhexidine gluconate, sulfamine, mercurochrome, lactoferrin or a hydrolyzate thereof, alkyl diaminoethyl glycine chloride solution, triclosan, sodium hypochlorite, chloramine T, salami powder, iodine compound, iodoform, sorbin Acid or a salt thereof, propionic acid or a salt thereof, salicylic acid, dehydroacetic acid, parahydroxybenzoic acid esters, undecylenic acid, thiamine lauryl sulfate, thiamine lauryl nitrate, phenol, cresol, p-chlorophenol, p-chloro-m -Xylenol, p-chloro-m-cresol, thymol, phenethyl alcohol, O-phenylphenol, Irgasan CH3565, halocarban, hexachlorophen, chlorohexidi , Ethanol, methanol, isopropyl alcohol, benzyl alcohol, ethylene glycol, propylene glycol, 2-phenoxyethanol, 1,2-pentanediol, zinc pyridione, chlorobutanol, isopropylmethylphenol, nonionic surfactant (polyoxyethylene lauryl ether , Polyoxyethylene nonyl phenyl ether, polyoxyethylene octyl phenyl ether, etc.), amphoteric surfactants, anionic surfactants (sodium lauryl sulfate, lauroyl sarcosine potassium, etc.), cationic surfactants (cetyltrimethylammonium bromide, benza chloride) Ruconium, benzethonium chloride, methylrosaniline chloride), formaldehyde, hexamine, brilliant green, malachite green, Star Le violet, Jamaru photosensitive No. 101-containing, photosensitizer No. 201, photosensitizer No. 401, N- long chain acyl basic amino acid derivative and its acid shark salts, zinc oxide, hinokitiol, Sophora root, propolis, and the like.
[0065]
(29) Moisturizer
Glycerin, propylene glycol, 1,3-butylene glycol, polyethylene glycol, glycerin tricaprylcaprate, glycolic acid (α-hydroxy acid), hyaluronic acid or its salt, chondroitin sulfate or its salt, water-soluble chitin or its derivative or chitosan derivative , Pyrrolidonecarboxylic acid or a salt thereof, sodium lactate, urea, sorbitol, amino acid or a derivative thereof (valine, leucine, isoleucine, threonine, methionine, phenylalanine, tryptophan, lysine, glycine, alanine, asparagine, glutamine, serine, cysteine, cystine, Tyrosine, proline, hydroxyproline, aspartic acid, glutamic acid, hydroxylysine, arginine, ornithine, histidine and their sulfates, Acid, nitrate, citrate, or pyrrolidone carboxylic acid), oils and fats such as avocado oil, almond oil, fennel oil, perilla oil, olive oil, orange oil, orange raffer oil, sesame oil, cocoa butter, chamomile oil, carrot oil, Cucumber oil, beef tallow fatty acid, quince seed oil, kukuinut oil, safflower oil, shea butter, liquid shea butter, soybean oil, camellia oil, corn oil, rapeseed oil, jojoba oil, persic oil, castor oil, sunflower oil, cottonseed oil, Peanut oil, turtle oil, tea tree oil, mink oil, egg yolk oil, palm oil, palm kernel oil, mokurou, coconut oil, eucalyptus oil, tallow, lard, squalene, squalane, pristane or hydrogenated products of these oils (hardened) Oil) ", waxes (bee wax, carnauba wax, whale wax, lanolin, liquid lanolin, reduced lanolin) , Hard lanolin, candelilla wax, montan wax, shellac wax, rice wax), mineral oil (liquid paraffin, vaseline, paraffin, ozokeride, ceresin, microcrystalline wax), natto metabolite, natto extract, silk fiber extract, plant extract (Akamatsu sap, aloe, kakkon, chamomile, licorice, cucumber, licorice, rice or rice bran, purple root, birch or birch sap, assemblage, mulberry bark, biwa leaf, loofah, peony, penguin) and the like.
[0066]
(30) Elastase activity inhibitor
Diisopropyl fluorophosphate, plant extracts (Ougon, Hypericum perforatum, Clara, Mulberry leaf, Calyx, Gennoshoko, Comfrey, Salvia, Agaricus japonicus, Bodaiju, Botanpi), seaweed extract and the like.
[0067]
(31) Hair agent
Selenium disulfide, alkylisoquinolinium bromide solution, zinc pyrithione, biphenamine, thianthol, castal tincture, ginger tincture, capsicum tincture, quinine hydrochloride, strong aqueous ammonia, potassium bromate, sodium bromate, thioglycolic acid and the like.
[0068]
(32) Antiandrogen agent
Estrogen (estrone, estradiol, ethinylestradiol, etc.), isoflavones, oxendrone, etc.
[0069]
(33) Peripheral vascular blood flow promoter
Vitamin E or its derivatives, assembly extract, garlic extract, ginseng extract, aloe extract, gentian extract, touki extract, cepharanthin, carpronium chloride, minoxidil and the like.
[0070]
(34) Stimulant
Pepper tincture, nonyl acid vanillamide, cantaris tincture, ginger tincture, peppermint oil, l-menthol, camphor, benzyl nicotinate and the like.
[0071]
(35) Metabolic activator
Photosensitizer 301, hinokitiol, pantothenic acid or derivatives thereof, allantoin, placental extract, biotin, glyceride pentadecanoate, and the like.
[0072]
(36) Antiseborrheic
Pyridoxine or a derivative thereof, sulfur, vitamin B6 and the like.
[0073]
(37) Keratolytic agent
Resorcinol, salicylic acid, lactic acid, urea, etc.
[0074]
(38) Oxidizing agent
Hydrogen peroxide solution, sodium persulfate, ammonium persulfate, sodium perborate, urea peroxide, sodium percarbonate, sodium tripolyphosphate, sodium bromate, potassium bromate, sodium pyrophosphate, sodium orthophosphate , Sodium silicate hydrogen peroxide adduct, sodium sulfate hydrogen peroxide adduct, sodium chloride hydrogen peroxide adduct, β-tyrosinase enzyme solution, mushroom extract and the like.
[0075]
(39) Hair remover
Inorganic reducing agents such as strontium sulfate, sodium sulfide, barium sulfide and calcium sulfide; thioglycolic acid or salts thereof (calcium thioglycolate, sodium thioglycolate, lithium thioglycolate, magnesium thioglycolate, strontium thioglycolate) etc.
[0076]
(40) Hair swelling agent
Ethanolamine, urea, guanidine, etc.
[0077]
(41) Dye agent
5-aminoorthocresol, 2-amino-4-nitrophenol, 2-amino-5-nitrophenol, 1-amino-4-methylaminoanthraquinone, 3,3'-iminodiphenol, 2,4-diaminophenoxyethanol hydrochloride 2,4-diaminophenol hydrochloride, toluene-2,5-diamine hydrochloride, nitroparaphenylenediamine hydrochloride, paraphenylenediamine hydrochloride, N-phenylparaphenylenediamine hydrochloride, metaphenylenediamine hydrochloride, orthoaminophenol, N-phenyl acetate Paraphenylenediamine, 1,4-diaminoanthraquinone, 2,6-diaminopyridine, 1,5-dihydroxynaphthalene, toluene-2,5-diamine, toluene-3,4-diamine, nitroparaphenylenediamine, paraaminophenol P-nitroorthophenylenediamine, p-phenylenediamine, p-methylaminophenol, picramic acid, sodium piclamate, N, N'-bis (4-aminophenyl) -2,5-diamino-1,4-quinonediimine, 5- ( 2-hydroxyethylamino) -2-methylphenol, N-phenylparaphenylenediamine, metaaminophenol, metaphenylenediamine, 5-aminoorthocresol sulfate, 2-amino-5-nitrophenol sulfate, orthoaminophenol sulfate, sulfuric acid Orthochloroparaphenylenediamine, 4,4'-diaminodiphenylamine sulfate, 2,4-diaminophenol sulfate, toluene-2,5-diamine sulfate, nitroparaphenylenediamine sulfate, paraaminophenol sulfate, paranitrophenol sulfate Tophenylenediamine, paranitrometaphenylenediamine sulfate, paraphenylenediamine sulfate, paramethylaminophenol sulfate, metaaminophenol sulfate, metaphenylenediamine sulfate, catechol, diphenylamine, α-naphthol, hydroquinone, pyrogallol, phloroglucin, gallic acid, resorcinol , Tannic acid, sodium 2-hydroxy-5-nitro-2 ', 4'-diaminoazobenzene-5'-sulfonate, hematein and the like.
[0078]
(42) Fragrance
Natural animal flavors such as musk, civet, castorium, ambergris, anise essential oil, angelica essential oil, ylang ylang essential oil, iris essential oil, fennel essential oil, orange essential oil, cananga essential oil, caraway essential oil, cardamom essential oil, guaiac wood essential oil, cumin essential oil, black character Essential oils, cinnamon essential oil, cinnamon essential oil, geranium essential oil, copaiba balsam essential oil, coriandel essential oil, perilla essential oil, cedarwood essential oil, citronella essential oil, jasmine essential oil, ginger grass essential oil, cedar essential oil, spearmint essential oil, western mint essential oil, large perfume essential oil, Tuberose essential oil, T-shaped essential oil, orange flower essential oil, winter green essential oil, true balsam essential oil, baturi essential oil, rose essential oil, palmarosa essential oil, cypress essential oil, hiba essential oil, sandalwood essential oil, petitglen essential oil, bay essential oil, vetiver essential oil, bergamot essential oil, perl Vegetable flavors such as balsam essential oil, boad rose essential oil, camphor essential oil, mandarin essential oil, eucalyptus essential oil, lime essential oil, lavender essential oil, linaloe essential oil, lemongrass essential oil, lemon essential oil, rosemary (mannen wax) essential oil, Japanese mint essential oil, etc. Synthetic fragrances, etc.
[0079]
(43) Dyes / colorants
Red cabbage pigment, red rice pigment, Akane pigment, anato pigment, squid pigment, turmeric pigment, endju pigment, krill pigment, persimmon pigment, caramel, gold, silver, gardenia pigment, corn pigment, onion pigment, tamarind pigment, spirulina pigment, Buckwheat whole plant pigment, cherry pigment, laver pigment, hibiscus pigment, grape juice pigment, marigold pigment, purple potato pigment, purple yam pigment, lac pigment, rutin, etc.
[0080]
(44) Sweetener
Sugar, sweet tea, fructose, arabinose, galactose, xylose, stevia, mannose, maltose, honey, glucose, miraculin, monelin, licorice extract and the like.
[0081]
(45) Nutrition enhancer
Shell calcined calcium, cyanocorabamine, yeast, wheat germ, egg yolk powder, hemicellulose, heme iron and the like.
[0082]
(46) Dairy products
Milk, cheese, fresh cream, butter, margarine, milk powder, whey, condensed milk, etc.
[0083]
(44) Other
Hormones, sequestering agents, pH adjusters, chelating agents, preservatives / antibiotics, cooling agents, stabilizers, emulsifiers, animal / vegetable proteins and their degradation products, animal / vegetable polysaccharides and their degradation products , Animal and plant glycoproteins and their degradation products, blood flow enhancers, anti-inflammatory agents, anti-inflammatory agents, anti-allergic agents, wound healing agents, foaming agents, thickening agents, enzymes, purified water (electronic water, small clusters) Etc.), oral agents, deodorants / deodorants, bittering agents, seasonings, etc., are expected to be added or synergistically improved in the effects of the present invention or other cosmetic effects when used in combination therewith. it can.
[0084]
Incidentally, the method of adding the hyaluronic acid production promoter or skin external preparation of the present invention to the food or drink may be added in advance or may be added during the production, and may be appropriately selected in consideration of workability. good.
[0085]
【Example】
Hereinafter, the present invention will be described with reference to Production Examples, Test Examples, and Formulation Examples, but the present invention is not limited thereto.
[0086]
(Production Example 1)
50 g of high-purity gelatin (manufactured by Miyagi Chemical Industry Co., Ltd.) was dissolved in 1,000 mL of 20 mM Tris-HCl buffer (pH 7.4) /0.1 M NaCl while heating, and then cooled to 50 ° C. The immobilized enzyme for enzymatic degradation was prepared by binding 100 mg of collagenase enzyme (high-purity purified from type IV, manufactured by Washington, Inc.) to 50 g of a particle carrier. The amount of binding to the carrier was calculated by measuring the change in absorbance at 280 nm before and after binding, and the binding rate was 99% or more. At the time of use, the immobilized enzyme is packed in a double column type bioreactor in which a pH sensor is installed between columns, and a 20 mM Tris-HCl buffer (pH 7.4) /0.1 M NaCl buffer is used. Then, washing and equilibration were performed well. The pH is measured by a system in which a pH sensor installed between the columns of the double column senses a change and a concentrated Tris buffer flows from a connected tube.
[0087]
The high-purity collagen obtained in the above step was applied to the vertical double-column collagenase enzyme-immobilized column prepared in the above step, and subjected to enzymatic degradation by a column method. During this time, the flow rate was controlled at 20 to 60 mL / min, and the column temperature was controlled at 39 ± 1 ° C. The enzyme reaction-terminated liquid that came out of the final (second column) column was collected and filtered with a 0.45 μm filter.
[0088]
This filtrate was powdered by spray drying (spray dryer), and then a collagen peptide having a tripeptide content of at least 10% or more was obtained by gel filtration using a gel filter (trade name: Superdex 30 pg, manufactured by Pharmacia). Was purified. The purified collagen peptide showed a peak at a molecular weight of about 280 due to the tripeptide. The tripeptide content can be increased by purification, and a collagen peptide having a tripeptide content of 80% or more can be obtained.
[0089]
After freeze-drying the collagen peptide obtained in the above step, that is, the collagen peptide having a tripeptide content of 10% or more, each NH 3 ₂ Terminal amino acid and NH ₂ The second amino acid from the terminal was assayed by the Edman degradation method. As a result, NH ₂ It was found that 97.5% of each terminal amino acid was glycine, which proved that the amino acid retained a (Gly-XY) n structure which is a characteristic amino acid sequence.
[0090]
(Test 1) Hyaluronic acid production promoting action test
"Test method and evaluation method"
Each well of a 24-well plate (Corning 3526) contains 5 × 10 ⁴ (Cell / mL) 1 mL of normal human neonatal foreskin skin fibroblast NHDF (NF) was seeded. Using a DMEM medium (manufactured by Kurabo Industries) containing 5% FCS (fetal calf serum) at 37 ° C., 5% CO 2 ₂ For 3 days, the medium was replaced with a DMEM medium containing 0.5% FCS, and a sample was added. Furthermore, the amount of hyaluronic acid (HA) was measured by ELISA using the culture supernatant after culturing for 24 hours as a sample. At the same time, the number of cells was counted and evaluated as the amount of hyaluronic acid per cell. Note that purified water was used as a blank and N-acetylglucosamine was used as a positive control, and the final concentration was 0.1 mM in a test container. The collagen peptide of the present invention was added to the test container to a final concentration of 10 ppm. FIG. 1 shows the result.
"sample"
In the test sample, the pig-derived collagen peptide and the shark-derived collagen peptide obtained in Production Example 1 of the present invention were adjusted to a solid content of 0.1% and then subjected to the test.
[0091]
(Test results)
As shown in FIG. 1, it was confirmed that the swine-derived collagen peptide and the shark-derived collagen peptide of the present invention have an effect of significantly promoting the production of hyaluronic acid.
[0092]
(Test 2) Skin roughness improvement action test
In order to evaluate the improvement effect of the product of the present invention on rough skin, a sample application test was performed using a guinea pig having a rough skin model. The sample was prepared by drying the pig-derived collagen peptide and the shark-derived collagen peptide obtained in Production Example 1, and re-dissolving the obtained solid in purified water so as to have a solid concentration of 0.1%. As a comparison, only purified water was used.
[0093]
"Method"
To a Hartley-type guinea pig (female, 5 weeks old, 3 animals per group) with depilated back, sodium lauryl sulfate (0.2 g) adjusted to 3% by mass with white petrolatum was continuously released for 3 days to remove rough skin. Created. The rough skin preparation site was divided into four minutes, each sample (1.0 mL) was applied three times / day, and three days later, the degree of rough skin was evaluated according to a criterion (score) determined by observation. The results are shown in Table 1.

[0094]
<Table 1>

[0095]
As shown in Table 1, it was confirmed that the collagen peptide of the present invention was effective in improving skin roughness.
[0096]
(Test 3) Safety test
(1) Skin primary irritation test
The collagen peptide obtained in Production Example 1 was prepared so as to have a solid content of about 2.0%, and the skin was shaved on the back of a Hartley-type guinea pig (female, 3 per group, weighing 300 to 350 g). 2x2cm ² 0.03 mL per square of was applied. The evaluation was performed using erythema and edema as indices by the primary irritation scoring method at 24, 48 and 72 hours after application. As a result, in all animals, no erythema and edema were observed, and the animals were determined to be negative.
[0097]
(Test 4) Safety test
(2) Skin irritation test
The collagen peptide obtained in Production Example 1 was prepared so as to have a solid concentration of about 2.0%, and the flank was shaved (2 × 4 cm). ² ) Was applied to the skin of Hartley-type guinea pigs (female, 3 animals per group, body weight of about 320 g) once a day, 5 times a week, 0.5 mL / animal. Application was over two weeks and shaving was performed on the last application day of each week. The determination was made on the day following each application day and the day after the last application day by the primary irritation scoring method using erythema and edema as indices. As a result, in all animals, no erythema and edema were observed for 2 weeks after application, and the animals were judged to be negative.
[0098]
(Test 5) Safety test
(3) Single dose toxicity test
Production Example 1 The obtained collagen peptide was adjusted with purified water to a concentration of 12.5%, and a 16 ml / kg amount per animal was fasted for 16 hours before the test, and ddy mice (male and female, A group of 5 animals, 5 weeks old) was orally administered with a sonde, and the appearance and degree of toxicity symptoms were observed over time. As a result, no abnormality was observed in all the mice for 14 days, and no abnormalities were found in the dissection results. Therefore, LD ₅₀ Is 2,000 mg / kg or more, which was judged to be a safe substance.
[0099]
(Prescription example) Manufacture of hyaluronic acid production promoter, skin external preparation and food and drink
In accordance with the above evaluation results, formulation examples are shown below. Each formulation example may be one manufactured by a usual method for manufacturing each product, and only the content is shown. Further, the present invention is not limited to these.
[0100]

[0101]

[0102]

[0103]

[0104]

[0105]

[0106]

[0107]

[0108]

[0109]

[0110]

[0111]

[0112]

[0113]

[0114]

[0115]

[0116]

[0117]

[0118]

[0119]

[0120]

[0121]

[0122]

[0123]

[0124]

[0125]

[0126]

[0127]

[0128]
(Formulation Example 29) Coating protective agent
A gauze or liniment cloth is impregnated with a formulation liquid prepared by mixing an appropriate amount of a pig-derived collagen peptide and a shark-derived collagen peptide of Example 1, an antibiotic, an anti-inflammatory agent, and the like, and attached to the wound. Alternatively, the pig-derived collagen peptide and the shark-derived collagen peptide of Production Example 1 may be directly applied locally and covered with gauze or the like.
[0129]

[0130]

[0131]

[0132]

[0133]

[0134]

[0135]

[0136]

[0137]

[0138]

[0139]
(Test 6) Use effect test
The effect when the skin external preparation of the present invention was actually used was examined. The use test was performed by applying a healthy amount of the milky lotion of Formulation Example 1 to the face over three months after washing the face twice daily, morning and evening, with 10 healthy adult women 25 to 50 years old as panelists. . Incidentally, a control prepared by the same method except that the collagen peptide of the present invention was removed from the emulsion was used as a control. The evaluation was performed according to the following criteria. The results are shown in Table 2 and the numbers in the table represent the number of persons. No one complained of abnormal skin during the use period.
[0140]
"Effect of improving skin tension and gloss"
Effective: Skin tension and gloss increased.
Slightly effective: Skin tension and gloss increased slightly.
Ineffective: No change from before use.
[0141]
<Table 2>

[0142]
(Test results)
As shown in Table 2, it was confirmed that the emulsion containing the product of the present invention significantly provided skin firmness and luster, and had effects such as skin wrinkle reduction.
[0143]
(Test 7) Use effect test
The effect when the food of the present invention was actually used was examined. The usage test consisted of 10 healthy adult women, 25 to 50 years old, as panelists, who had three cookies (Prescription 50g at a time) twice daily in the morning and at night for one month. Was. As a control, a cookie prepared by removing the collagen peptide of the present invention from the cookie in the same manner was used. The evaluation was performed according to the following criteria. The results are as shown in Table 3 and the numerical values in the table represent the number of persons. No one complained of abnormal skin during the use period.
[0144]
"Effect of improving skin tension and gloss"
Effective: Skin tension and gloss increased.
Slightly effective: Skin tension and gloss increased slightly.
Ineffective: No change from before use.
[0145]
<Table 3>

[0146]
(Test results)
As shown in Table 3, it was confirmed that the cookie containing the product of the present invention significantly provided skin firmness and luster, and also reduced skin thickening.
[0147]
【The invention's effect】
It is a degradation product of collagen or gelatin of the present invention by collagenase, and its amino acid sequence is (Gly-XY) (where Gly represents a glycine residue, X and Y represent amino acid residues, and X and Y may be the same or different), the collagen peptide (Gly-XY) n (n represents a positive integer) consisting of a collagen tripeptide having high safety is high. , Which significantly promoted hyaluronic acid production and had an effect of improving skin roughness. Further, a skin external preparation or a food or drink containing them has an effect of preventing skin aging such as imparting firmness and luster to the skin, or improving wrinkles and tarmi.
[Brief description of the drawings]
BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a graph showing the hyaluronic acid production promotion rate of a pig-derived collagen peptide (designated as “shark-derived CP”) and a shark-derived collagen peptide (designated as “porcine-derived CP”) of the present invention.

Claims (3)

Collagenase degradation product of collagen or gelatin, whose amino acid sequence is (Gly-XY) (where Gly represents a glycine residue, X and Y represent amino acid residues, and X and Y Wherein the hyaluronic acid comprises a collagen peptide (Gly-XY) n (n represents a positive integer) consisting of a collagen tripeptide represented by the following formulas: Acid production promoter. An external preparation for skin, comprising the hyaluronic acid production promoter according to claim 1. A food or drink comprising the hyaluronic acid production promoter according to claim 1.

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