KR20170025370A - Composition for improving skin - Google Patents
Composition for improving skin Download PDFInfo
- Publication number
- KR20170025370A KR20170025370A KR1020150121711A KR20150121711A KR20170025370A KR 20170025370 A KR20170025370 A KR 20170025370A KR 1020150121711 A KR1020150121711 A KR 1020150121711A KR 20150121711 A KR20150121711 A KR 20150121711A KR 20170025370 A KR20170025370 A KR 20170025370A
- Authority
- KR
- South Korea
- Prior art keywords
- skin
- composition
- extract
- effect
- stem cell
- Prior art date
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2200/00—Function of food ingredients
- A23V2200/30—Foods, ingredients or supplements having a functional effect on health
- A23V2200/318—Foods, ingredients or supplements having a functional effect on health having an effect on skin health and hair or coat
Abstract
Description
The present invention relates to a skin improving composition exhibiting skin regeneration, elasticity enhancement, skin moisturizing, skin texture improvement, and stem cell activity promoting effect.
Collagen is a major substrate protein produced in fibroblasts of the skin and exists in extracellular epilepsy. Its important functions are mechanical rigidity of skin, resistance of connective tissues and binding force of tissues, support of cell adhesion, division of cells and differentiation Growth or wound healing) are known. Such collagen is reduced by aging and photo aging caused by ultraviolet irradiation, and it is known that it is closely related to elasticity of skin.
Effective substances that promote collagen synthesis and exhibit an elasticity-enhancing effect are known. For example, retinoic acid, transforming growth factor (TGF) [non-patent document 1], animal placenta-derived protein [Patent document 1], betulinic acid [Patent document 2], chlorella extract [ Patent Documents 3 and 4] are known as collagen synthesis promoting substances. However, the above-mentioned effective ingredients are limited in the use amount due to safety problems such as irritation and redness when applied to the skin, or have insufficient effect, so that the effect of improving the skin function by promoting the collagen synthesis of the skin can not be expected.
In addition, the epidermis located at the outermost part of the skin protects against various external physical, chemical and mechanical stimuli and protects against excessive divergence of body water through the skin. This protective function is possible by normally forming and maintaining the stratum corneum composed of keratinocytes. The keratinocyte is a cell formed by a stepwise change in morphology and function while a basal cell that continuously proliferates in the stratum basale moves to the stratum corneum, Forming cells are removed from the skin and the new keratinocytes from the epidermis's bottom layer repeat the process of epidermis differentiation or keratinization replacing its function. In this keratinization process, keratinocytes produce intercellular lipids such as natural moisturizing factors (NMF) and ceramides, cholesterol, and fatty acids, which act as barrier layers to the outside, As shown in Fig.
In addition, skin dryness, which is considered to be one of the major diseases of modern society, is one of the symptoms caused by skin barrier function abnormality. Recently, environmental pollution, increase in dry environment such as apartments and high rise building, increase in social stress, Of excessive bathing, skin aging, and the like, and the cases of severe symptoms and the need for treatment are also increasing steadily.
In addition, the skin texture, which is the shape of the skin surface, is characterized by a three-dimensional microstructure formed by fine lines and is significantly different according to age and site. The skin texture is divided into primary (about 20-100 μm), secondary (about 5-40 μm), and tertiary (about 0.5 μm) lines depending on the depth of the line. And the star formation is evident in the polygon and star formation that the lines meet [Non-Patent Document 2].
However, as the age increases or the skin becomes damaged, the network structure of the dense fine lines collapses and fine lines (secondary, tertiary, etc.) disappear and the primary lines become deeper and wrinkles are known to be produced Literature 3,4]. In other words, wrinkles, a typical sign of skin aging, are accumulations of minute changes that have already occurred over a long period of time.
Embryologically, all components of human skin are known to originate from ectoderm or mesodermal lobe. Epidermis, hair follicles, sebaceous glands and glands are originated from ectoderm, and melanocytes, nerves and special sensory receptors are derived from neuroepithelial cells. According to the developmental stage, the embryonic stem cells are repeatedly differentiated and become cells with the characteristics of each tissue function. After the embryo, a certain number of stem cells remain in the tissue. . The first is in the hair follicle. It is known to play an important role in the regeneration of the epidermis into cells before cell differentiation occurs, and plays an important role in hair regeneration and growth. The second is the basal layer of the epidermis. The stem cells found here play an important role in maintaining skin health by administering not only the epidermis but also the fibroblasts of the dermal layer. The stem cells here are relatively large in quantity and easy to obtain, making them widely used in the study of skin stem cells. The skin is constantly renewed, and stem cells present in the epithelium of the skin, that is, epidermal stem cells of the skin, are involved in the repair of the epithelium after injury [Non-Patent Document 5]. The epithelial stem cells of the skin are also referred to as skin stem cells. When the skin stem cells are activated, it is possible to treat skin wounds such as trauma, promote wound healing, have. Integrin β1 and integrin α6 are used as indicators of dermal stem cells, and maintenance of skin stem cells necessary for epithelial morphogenesis and differentiation has been reported to be regulated by p63 protein.
Dermal stem cells play an important role in maintaining the health and physiological and biochemical homeostasis of the skin. Stem cells present in the skin are also abnormally functioned due to the effect of aging, and thus various problems arise as the homeostasis of the skin is broken. Therefore, it is possible to improve various phenomena of skin aging through the activation of stem cells [Non-Patent Document 6].
In addition, there is a desperate need for the development of a composition which is safe in the living body, stable in its active ingredient, and most effective in improving the elasticity, skin moisturizing effect and skin texture improving activity, Is desired.
On the other hand, TYPHAE POLLEN (蒲黄) is a medicinal herb which is obtained by extracting mature pollen of Typha latifolia L., Typha orientalisPresl, Typha angustifolia L. . It is light and fine powder, light yellow to yellowish brown. When observed under a microscope, four pollen grains are combined in a square or ladder shape, and the diameter is 35 to 40 μm (Park Jong Hee, 867-868). According to the Dongbuobogam (汤液 篇) and the 草 部 (草 部), the ingredients are uniform, flavorless, poisonous, stop the bleeding of 九규, bloody blood, It treats the Bongbu Daejang and the morning sickness, the lower blood, and the tongue. It is said that it is in the pond water and it is in various places, that is, it is the sulfur powder in the seedlings, and it falls in the period when the powder is formed (Koo Bon-hong, first edition of Dongbokgol, Korea Education and Culture History, 1411).
As described above, various pharmacological effects of boiling have been reported, but the mechanism of the boiling of the skin has not yet been known, and no studies have been conducted on it.
The present inventors have found that the Chinese cabbage extract promotes the proliferation of skin stem cells, promotes skin regeneration, promotes collagen synthesis of fibroblasts of the skin to promote elasticity, exhibits skin moisturizing effect due to increased fila green expression, The present invention has been accomplished by confirming the effect of stimulating stem cell activity because it promotes the proliferation of skin stem cells and exhibits a stem cell maintenance effect.
Accordingly, an object of the present invention is to provide a composition for skin regeneration, elasticity enhancement, skin moisturization, and skin texture improvement, which comprises a bolus extract as an active ingredient.
Another object of the present invention is to provide a composition for promoting stem cell activity comprising the following components as an active ingredient.
As a means for solving the above problems, the present invention provides a composition for skin regeneration, elasticity enhancement, skin moisturization and skin texture improvement, which comprises, as an active ingredient, a bolus extract for the production of medicines, cosmetics or foods.
As another means for solving the above-mentioned problems, the present invention provides a composition for promoting stem cell activity comprising, as an active ingredient, a bovine extract for the production of cosmetic formulations.
The bamboo extract according to the present invention promotes the proliferation of skin stem cells, promotes regeneration of skin, promotes collagen synthesis of fibroblasts of skin to promote skin elasticity, and also has an effect of improving skin moisturization due to an increase in pilar green expression, And exhibits an effect of improving the skin texture due to the improvement in brightness, so that it can be used in the manufacture of medicines, cosmetics or foods.
In addition, the bovine extract promotes the proliferation of dermal stem cells and exhibits a stem cell-retaining effect, and thus can be used for the production of cosmetic formulations for promoting stem cell activity.
Hereinafter, the configuration of the present invention will be described in detail.
In order to exert an excellent effect when the skin regeneration, elasticity enhancement, skin moisturizing and skin texture improving ingredient is applied to the actual skin, it exhibits highly active skin regeneration, elasticity enhancement, skin moisturizing and skin texture improving activity, Is low in volatility so as to be able to stay for a sufficient time to exhibit skin regeneration, elasticity enhancement, skin moisturization and skin texture improving effect and is capable of stably maintaining the active ingredient on the composition or skin, Etc., and it is preferable that it is safe for the skin. However, the components satisfying all of the above-mentioned characteristics among the known components are not common. For example, some skin regeneration, elasticity enhancement, skin moisturizing and skin texture improving agents are excellent in skin regeneration, elasticity enhancement, skin moisturizing and skin texture improving activity even at a low concentration in an in vitro test, It is difficult to apply to real skin. Other active ingredients have low hydrophilicity, making them difficult to formulate into medicines, cosmetics, and foods. In addition, some skin regeneration, elasticity enhancement, skin moisturizing and skin texture improving agents may be degraded or transformed into other compounds when they are exposed to heat, light, or oxygen, .
As can be seen in the following examples, the extract of Choshu exhibits remarkably excellent proliferation promoting effect of collagen, expression of pillar green, and improvement of skin brightness, which are superior in low density. Therefore, the regeneration of skin, the enhancement of elasticity, And as medicines, cosmetics or food compositions for improving skin texture.
Accordingly, the present invention provides a composition for skin regeneration, elasticity enhancement, skin moisturization, and skin texture improvement, comprising a bolus extract as an active ingredient.
In the present invention, TYPHAE POLLEN (蒲alkon) is obtained by extracting mature pollen of Typha latifolia L., Typha orientalisPresl, Typha angustifolia L., to be.
The above-mentioned bolus extract can be extracted by a method known in the art, and the method is not particularly limited. Alternatively, commercially available extracts can be used.
Preferably, the extract is obtained by extracting with a water and / or an organic solvent, and the organic solvent may be a polar organic solvent, a nonpolar organic solvent or a mixture thereof. The polar organic solvent may be a lower alcohol having 1 to 5 carbon atoms, ethyl acetate or acetone, and the nonpolar organic solvent may be ether, chloroform, benzene, hexane or dichloromethane. For example, the lower alcohol having 1 to 5 carbon atoms may be methanol, ethanol, propanol, butanol or isopropanol.
In one embodiment, the cholesterol extract may include a fraction obtained by fractionating the primary extract extracted using the above-described extraction solvent with an extraction solvent having a different polarity. For example, the sulfur extract may be a fraction obtained by extracting with an alcohol having 1 to 5 carbon atoms and then fractionating again with a solvent having a different polarity such as ether, benzene or hexane. Two or more kinds of solvents may be used for the fractionation, and the solvent extracts may be sequentially used or mixed according to the polarity of the solvent.
In the present invention, the extracted extract or the fraction obtained by performing the fractionation process may be filtered, concentrated or dried to remove the solvent, and the filtration, concentration, and drying may all be performed. Specifically, the filtration can be performed using a filter paper or a vacuum filter, and the concentration can be reduced by using a reduced pressure concentrator or the like, and the freeze-drying method can be carried out for drying.
The fractions obtained by performing the above-described extract or the fractionation process can be further purified by silica gel column chromatography, thin layer chromatography or high performance liquid chromatography Further purification can be achieved by purification using various chromatographic techniques.
The compositions of the present invention may be used for the manufacture of pharmaceutical formulations.
The pharmaceutical formulations may be in the form of solutions, suspensions or emulsions in oils or aqueous media, or in the form of excipients, powders, granules, tablets or capsules.
In addition, the composition may further contain one or more active ingredients showing the same or similar functions. For example, it may contain known skin regeneration, elasticity enhancement, skin moisturizing and skin tone improving ingredients. The addition of additional skin regeneration, elasticity enhancement, skin moisturizing and skin texture improving ingredients may further enhance the skin regeneration, elasticity enhancement, skin moisturization and skin texture improvement effects of the composition of the present invention. When the above ingredients are added, skin safety, easiness of formulation, and stability of effective ingredients can be considered according to the combined use. In one embodiment of the present invention, the composition is a skin regeneration component known in the art comprising retinoic acid, TGF, protein from animal placenta, betulinic acid and chlorella extract, antioxidant components known in the art, such as tocopherol, selenium , Vitamin C and phenolic compounds, whitening ingredients known in the art, substances inhibiting the activity of tyrosinase enzymes such as kojic acid and arbutin, hydroquinone, vitamin C (L- Ascorbic acid, derivatives thereof, and various plant extracts. The additional ingredients may be included in an amount of 0.0001 wt% to 10 wt% based on the total weight of the composition, and the content range may be adjusted according to requirements such as skin safety, ease of formulating the bolus extract, and the like.
In addition, the composition of the present invention may further comprise a pharmaceutically acceptable carrier.
Pharmaceutically acceptable carriers may contain a variety of ingredients such as buffer, injectable sterile water, normal saline or phosphate buffered saline, sucrose, histidine, salts and polysorbates, and the like.
The composition of the present invention can be administered orally or parenterally, and can be administered in the form of a general pharmaceutical preparation, for example, various forms of oral and parenteral administration at the time of clinical administration. In the case of formulation, a filler, , A binder, a wetting agent, a disintegrant, a surfactant, and the like.
Solid formulations for oral administration include tablets, pills, powders, granules, capsules and the like. These solid preparations can be prepared by mixing the pharmaceutical composition of the present invention with at least one excipient such as starch, calcium carbonate, Sucrose, lactose, gelatin and the like can be prepared.
In addition to simple excipients, lubricants such as magnesium stearate talc are also used. Examples of liquid formulations for oral administration include suspensions, solutions, emulsions, syrups and the like. Various excipients such as wetting agents, sweeteners, fragrances, preservatives and the like may be included in addition to water and liquid paraffin, which are simple diluents commonly used.
Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. Propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like can be used as the non-aqueous solvent and suspension agent. Examples of the suppository base include witepsol, macrogol, tween 61, cacao butter, laurin, glycerogelatin and the like.
In the present invention, the term 'skin regeneration effect' refers to the recovery of skin tissue against damage caused by external or internal causes of skin as the activity of skin stem cells is promoted. At this time, the damage caused by the external cause may include ultraviolet rays, external pollutants, wound or trauma, and the damage caused by the internal cause may be stress.
In the present invention, the 'elasticity-enhancing effect' refers to an increase in elasticity to the skin, which suppresses or inhibits the loss of elasticity of the skin, or alleviates the already-reduced elasticity.
In the present invention, the term 'moisturizing effect' refers to inhibiting or reducing the reduction of moisture in the skin, or increasing the moisture content of the skin to smooth and smooth the surface of the skin.
In the present invention, the term " skin texture improving effect " refers to smoothing the skin surface, imparting gloss, and brightening the skin tone by inhibiting or inhibiting roughness of the skin surface due to aging, stress, .
In the present invention, the term "effective amount" means the amount of the extract capable of promoting regeneration of damaged skin, improving elasticity, improving moisturizing effect, and improving skin texture. When the composition of the present invention contains the effective amount of the above-described cholesterol extract, it is possible to provide a desirable skin regeneration effect, a resilience enhancing effect, a moisturizing effect and a skin texture improving effect. The effective amount of the above-described bolus extract contained in the composition of the present invention will vary depending on the form in which the composition is made into a product, the method in which the compound is applied to the skin, and the time on the skin. For example, when the composition is commercialized as a pharmaceutical formulation, it may contain a higher concentration of the extract than the cosmetic product that is routinely applied to the skin. Accordingly, the daily dosage is 0.1 to 100 mg / kg, preferably 30 to 80 mg / kg, more preferably 50 to 60 mg / kg, and 1 to 6 times / day, ≪ / RTI >
The composition of the present invention may be used alone or in combination with methods using surgery, radiation therapy, hormone therapy, chemotherapy, and biological response modifiers.
The pharmaceutical formulations of the present invention may include external preparation for skin.
When the above-described bolus extract is used as an external preparation for skin, it may further contain at least one selected from the group consisting of fatty substances, organic solvents, solubilizers, thickening and gelling agents, softening agents, antioxidants, suspending agents, stabilizing agents, foaming agents, , Ionic or nonionic emulsifiers, fillers, sequestering agents and chelating agents, preservatives, vitamins, barrier agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or external preparations for skin ≪ / RTI > any other ingredients that are commonly used in the skin sciences. The components can also be introduced in amounts commonly used in the field of dermatology.
When the bolus extract is provided as an external preparation for skin, it may have a formulation such as, but not limited to, ointments, patches, gels, creams or sprays.
In addition, the composition for improving skin regeneration, elasticity enhancement, skin moisturizing and skin texture of the present invention can be used for the production of cosmetic formulations.
The cosmetic formulation may be in the form of a conventional emulsion formulation and a solubilized formulation. For example, creams, essences, cosmetic creams, sprays, gels, packs, sunscreens, make-up bases, liquids such as lotions such as lotion, facial lotion, body lotion, A powder, a cleansing lotion, a makeup removing agent such as a cleansing oil, a cleansing foam, a soap, a body wash, and the like.
In addition, the cosmetic composition may further contain, in addition to the sulfuric extract, a lipid, an organic solvent, a solubilizing agent, a thickening agent and a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizer, a foaming agent, a fragrance, Or any other commonly used in cosmetics such as nonionic emulsifiers, fillers, sequestering and chelating agents, preservatives, vitamins, barrier agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or cosmetics ≪ RTI ID = 0.0 > ingredients. ≪ / RTI >
The cosmetic formulation may contain a relatively high concentration of the above-described bolus extract in the case of a wash-off type cosmetic such as a make-up remover, a detergent, etc. in which the active ingredient remains on the skin in a short period of time. On the other hand, in the case of leave-on type cosmetics such as lotion, cream, essence and the like in which the active ingredient remains on the skin for a long period of time, It will be acceptable. In one embodiment of the present invention, but not limited thereto, the composition may comprise 0.0001 wt% to 10 wt% (preferably 0.0001 wt% to 1 wt%) of the bolus extract, based on the total weight of the composition . When the composition of the present invention contains less than 0.0001% by weight of the above-described extract, the effect of skin regeneration, elasticity enhancement, skin moisturization and skin texture improvement can not be expected sufficiently. It is intended to prevent this reaction because it may cause a reaction or skin safety problem.
Further, the composition for improving skin regeneration, elasticity enhancement, skin moisturizing and skin texture of the present invention can be used for the production of food formulations.
The food formulation refers to a food prepared by adding the above-described bolus extract to a food material such as beverage, tea, spice, gum, confectionery, or the like, or by encapsulation, powdering, suspension or the like.
Since the food preparation can be ingested routinely, it is very useful because high skin regeneration, elasticity enhancement, skin moisturization and skin texture improvement effect can be expected.
When the above-mentioned chorioallantoic extract is used as a food additive, the above-mentioned chorus extract may be directly added or used together with other food or food ingredients, and suitably used according to a conventional method. The amount of the active ingredient to be mixed can be suitably determined according to its intended use (prevention, health or therapeutic treatment). Generally, the composition of the present invention is added in an amount of not more than 15 parts by weight, preferably not more than 10 parts by weight, based on the raw material, when the food or beverage is produced. However, in the case of long-term consumption intended for health and hygiene purposes or for health control purposes, the amount may be less than the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount exceeding the above range .
There is no particular limitation on the kind of the food. Examples of foods to which the above substances can be added include dairy products including meats, sausages, breads, chocolates, candies, snacks, confections, pizza, ramen noodles, gums, ice cream, soups, drinks, tea, Alcoholic beverages, and vitamin complexes, all of which include health foods in a conventional sense.
When the food is a beverage, various flavors or natural carbohydrates may be added as an additional ingredient such as a normal drink. The above-mentioned natural carbohydrates are sugar alcohols such as monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, polysaccharides such as dextrin and cyclodextrin, and xylitol, sorbitol and erythritol. Examples of sweeteners include natural sweeteners such as tau martin and stevia extract, synthetic sweeteners such as saccharin and aspartame, and the like. The ratio of the natural carbohydrate is generally about 0.01 to 0.04 g, preferably about 0.02 to 0.03 g per 100 mL of the composition of the present invention.
In addition to the above, the food formulations may contain various nutrients, vitamins, electrolytes, flavors, colorants, pectic acid and salts thereof, alginic acid and its salts, organic acids, protective colloid thickeners, pH adjusters, stabilizers, preservatives, glycerin, And the like. Other food formulations may contain flesh for the production of natural fruit juices, fruit juice drinks and vegetable drinks. These components may be used independently or in combination. The proportion of such additives is not critical, but is generally selected in the range of 0.01 to 0.1 parts by weight per 100 parts by weight of the composition of the present invention.
The present invention also provides a composition for stimulating stem cell activity, comprising the above-described chorioallantoic extract as an active ingredient.
The term 'stem cell' in the present invention is a cell capable of cell division by itself and capable of differentiating into a very specific type of specific cell type. The type of such stem cells is not particularly limited, and in one embodiment, the stem cells may be dermal stem cells. The term 'dermal stem cells' refers to stem cells that can be differentiated into cells constituting the skin (epidermis, dermis and subcutaneous fat layer). The cells that make up the skin include keratinocytes, melanocytes, and fibroblasts (mainly responsible for biosynthesis of collagen and elastin) present in the epidermis.
The kind of the skin stem cell is not particularly limited. The dermal stem cells used in the present invention can be used irrespective of where they originate from. For example, dermal stem cells may be obtained from a known source of dermal stem cells, e. G., From the basal layer of hair follicles or epidermis, and the animal to be harvested may be a mammal. In one embodiment, the mammal may include, but is not limited to, a human, a mouse, a rat, a guinea pig, a rabbit, a monkey, a pig, a horse, a cattle, a sheep, Preferably the mammal can be human. Such methods of obtaining dermal stem cells from dermal stem cell sources are well known in the art.
In the present invention, the term 'promoting effect on stem cell activity' refers to an effect of promoting stem cell proliferation and / or an effect of maintaining stem cell characteristics, which is a specific indicator molecule and a self-cleaving property of stem cells.
In addition, the compositions of the present invention can be used for the production of cosmetic formulations. These cosmetic formulations are the same as those described in the description of cosmetic formulations including the above-mentioned burgundy extract.
Hereinafter, the present invention will be described in detail with reference to examples. However, the following examples are illustrative of the present invention, and the present invention is not limited to the following examples.
Manufacturing example One: Booming Preparation of extract
After boiling for 5 days in a 10-fold volume of ethanol solvent, the extract solution was filtered and concentrated to obtain an extract. To this was added a mixed solution of purified water and ethanol to dissolve and then filtered to prepare a final extract.
Manufacturing example 2: Serum-free Under medium conditions Of dermal stem cells culture
Human epidermal stem cells purchased from Cellntec were added to 48-well plates (6 × 10 3 cells / well), supplemented with BPE (bovine pituitary extract) similar to fetal bovine serum And cultured for 24 hours at 5% CO 2 and 37 ° C using CNT-57 medium (Cellntec). Then, the removal through the medium-introducing tube, and then, PBS solution (GibcoBRL Co.) to remove the medium components, processes the pohwang extract 0.0005 wt% in the CNT-57 medium that does not include BPE 5% CO 2 and 37 using Lt; 0 > C for 72 hours.
Experimental Example One: CCK -8 Through evaluation method Dermal stem cells Proliferation promoting effect
The skin stem cells cultured in Preparation Example 2 were evaluated for CCK-8 (Cell counting kit-8). The CCK-8 assay is an indirect method of measuring the density of living cells by measuring the absorbance of Formazan, a dehydrogenase in the intracellular electron transport system, produced by the decomposition of tetrazolium salt.
Cells were treated spectrophotometrically by treating CCK-8 solution (treated with 1/10 of the medium volume) at 37 ° C for 2 hours and then measuring the absorbance at 450 nm. The proliferation rate of the stem stem cells of the bovine extract was determined from the measured absorbance value in accordance with the following equation (1) with respect to the control group (CNT-57 medium supplemented with BPE), and the growth rate (%) was shown in Table 1 below.
[Equation 1]
Growth rate (%) = (absorbance of sample-treated group / absorbance of control group) x 100
As shown in Table 1, the dermal stem cells in the medium treated with the extract of burgdorfer showed excellent proliferation promoting effect.
Experimental Example 2: Of dermal stem cells Stem cell ( stemness ) Confirm maintenance effect
The expression level of p63 was evaluated in order to confirm the stem cell maintenance effect of the dermal stem cells cultured in Production Example 2 above. p63 is an intracellular transcription factor and is well known as a maintenance marker for dermal stem cells. Cells were isolated and cDNA was synthesized. Then, real-PCR was performed with Taqman staining solution to measure the expression level of p63. The concentration of RNA in this experiment was normalized to S16 ribosomal RNA.
The experimental results are shown in Table 2 below. In Table 2 below, the numerical value of the increase in p63 is a multiple of the control group (CNT-57 medium supplemented with BPE).
As shown in Table 2, the media conditions in which the bovine extract was treated promoted the expression of p63 in dermal stem cells, and thus the stem cell maintenance effect was excellent.
Example 1: Promoting collagen synthesis
Bovine extracts were added to the culture medium of human - derived fibroblasts to test the promoting effect of collagen synthesis at the cellular level. The biocompatible collagen was quantitated using a PICP EIA kit (Procollagen Type I C-Peptide Enzyme Immunoassay Kit).
(7 × 10 4 cells / cm 2 ), together with vitamin C and a control (no supplement), to a final concentration of 10 μg / mL and 20 μg / mL, After culturing, the culture was taken and the degree of collagen biosynthesis at each concentration was measured at 450 nm using a spectrophotometer with a PICP EIA Kit. The collagen biosynthesis performance was calculated by the relative performance relative to the control group and the results are summarized in Table 3 below.
As shown in Table 3, the bovine extract has superior collagen synthesis ability against human-derived fibroblasts and can obtain a better collagen synthesis effect at a lower concentration than that of vitamin C, which is generally known to have collagen synthesis ability .
Example 2: Filaggrin's Promoting effect of expression
After incubation for 1 day, the cell RNA was isolated and cDNA was synthesized. The cDNA was synthesized with Taqman staining solution and Real-Sepharose extract was added to the culture medium of human keratinocyte HaCaT cells for 5 days, time PCR. The RNA concentration was normalized to S16 ribosomal RNA.
As shown in Table 4, the Chinese cabbage extract has the ability to promote pilar green expression of human keratinocyte HaCaT cells.
Example 3: To improve the turn-over of the skin About Efficacy
About the nutritional cream of the following formulation example 2, the effect of the improvement of the exfoliation of the skin in the healthy twenties to 50 women was tested as follows.
Twenty of 20 women aged 20 to 50 years were given a 1.5% solution of DHA (Dihydroxyacetone, Sigma Aldrich, USA) for 8 hours, followed by deposition. The preparation cream was applied twice daily to the test site. After 4 days of application, 8 days after application, 12 days after application, photographs were taken using instrument Chromameter CR-400 (Minolta, Japan) and skin lightness of DSLR and DSLR. The measured values were evaluated by three mean values excluding the maximum value and the minimum value. The higher the improvement in the skin brightness of the deposition site, the better the improvement of the exfoliation. The results are shown in Table 5 below.
As shown in Table 5, when the nutritional cream according to the present invention was used, it was found that the exfoliation of the skin was improved.
Formulation example 1: Manufacture of pharmaceutical preparations
1. Preparation of tablets
Extract of chorus 0.2 mg
100 mg of corn starch
100 mg of milk
2 mg of magnesium stearate
After mixing the above components, tablets were prepared by tableting according to a conventional method for producing tablets.
Formulation example 2: Manufacture of cosmetics
1. Manufacture of nutritional cream
As shown in the following composition, a nutritional cream containing a cholesterol extract as an active ingredient was prepared by a conventional method.
0.2%
Beta-1,3-glucan 5.0 wt%
Wax 10.0 wt%
Polysorbate 60 1.5 wt%
≪ tb > < tb > < tb >
0.5% by weight of sorbitan sesquioleate
Liquid paraffin 10.0 wt%
Squalane 5.0 wt%
Caprylic / capric triglyceride 5.0 wt%
Glycerin 5.0 wt%
3.0% by weight of butylene glycol
3.0% by weight of propylene glycol
0.2% by weight triethanolamine
Preservative 0.05 wt%
0.05% by weight of pigment
0.05% by weight fragrance
Purified water to 100%
Formulation example 3: Preparation of external preparation for skin
1. Manufacture of ointment
As in the following composition, an ointment containing a bolus extract as an active ingredient was prepared according to a conventional method.
0.5%
Beta-1,3-glucan 10.0 wt%
Wax 10.0 wt%
Polysorbate 60 5.0 wt%
≪ tb > < tb > < tb >
0.5% by weight of sorbitan sesquioleate
Vaseline 5.0 wt%
Liquid paraffin 10.0 wt%
Squalane 5.0 wt%
SHARE BUTTER 3.0 wt%
Caprylic / capric triglyceride 5.0 wt%
Glycerin 10.0 wt%
Propylene glycol 10.2 wt%
0.2% by weight triethanolamine
Preservative 0.05 wt%
0.05% by weight of pigment
0.05% by weight fragrance
Purified water to 100%
Claims (13)
The composition for skin regeneration is an extract obtained by extracting a bouleum with at least one selected from the group consisting of water and an organic solvent.
The organic solvent may be a polar solvent containing a lower alcohol having 1 to 5 carbon atoms, ethyl acetate, or acetone; A nonpolar solvent comprising ether, chloroform, benzene, hexane or dichlorohexane; Or a mixed solvent thereof.
Wherein the composition is for the manufacture of a medicament for skin regeneration, a cosmetic preparation or a food product.
Wherein the composition is for the manufacture of a skin elasticity-enhancing drug, a cosmetic composition or a food product.
Wherein the composition is for skin moisturizing medicines, cosmetics or foods.
Wherein the composition is for the manufacture of a medicament for the improvement of skin texture, a cosmetic preparation or a food product.
Wherein the composition is for promoting stem cell proliferation or stem cell retention.
Wherein the composition is for the production of a cosmetic for promoting stem cell activity.
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| WO2018155997A1 (en) | 2017-02-27 | 2018-08-30 | 재단법인 대구경북과학기술원 | Use of erythropoietin-derived peptide through effect on cell damage prevention thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH08208424A (en) | 1994-12-20 | 1996-08-13 | Unilever Nv | Cosmetic composition containing betulinic acid |
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2015
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| JPH08208424A (en) | 1994-12-20 | 1996-08-13 | Unilever Nv | Cosmetic composition containing betulinic acid |
| JPH08231370A (en) | 1995-02-23 | 1996-09-10 | Taiyo Kagaku Co Ltd | Skin cosmetic |
| JPH0940523A (en) | 1995-07-28 | 1997-02-10 | Ichimaru Pharcos Co Ltd | Fibroblast proliferation promoter containing water extract form chlorella |
| JPH1036283A (en) | 1996-07-18 | 1998-02-10 | Ichimaru Pharcos Co Ltd | Fibroblast proliferation promoting agent |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018155997A1 (en) | 2017-02-27 | 2018-08-30 | 재단법인 대구경북과학기술원 | Use of erythropoietin-derived peptide through effect on cell damage prevention thereof |
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