KR20150087144A - Composition for improving skin - Google Patents

Abstract

The present invention relates to a composition for enhancing skin. According to the present invention, eleutheroside D promotes skin reproduction by promoting synthesis of collagen of fibroblast, alleviates wrinkles, promotes elasticity, shows an antioxidant effect by eliminating free radicals, has an anti-inflammation effect by inhibiting production of NO, shows an effect for improving skin moisturizing due to increase of expression of filaggrin, and shows skin appearance enhancement due to skin brightness improvement, thereby being used as a pharmaceutical composition, a skin external application, a cosmetic composition, or a health food. Also, the eleutheroside D promotes proliferation of skin stem cells and shows an effect of maintaining stem cells, thereby being used as a composition for promoting activity of stem cells and a cosmetic material comprising the same.

Description

[Composition for improving skin]

The present invention relates to a composition for improving skin such as skin regeneration, wrinkle improvement, elasticity enhancement, antioxidant, anti-inflammation, moisturizing and skin texture improvement.

Collagen is a major substrate protein produced in fibroblasts of the skin and exists in extracellular epilepsy. Its important functions are mechanical rigidity of skin, resistance of connective tissues and binding force of tissues, support of cell adhesion, division of cells and differentiation Growth or wound healing) are known. Such collagen is reduced by aging and photo aging caused by ultraviolet irradiation, which is known to be closely related to the wrinkling of the skin. Also, in recent years, extensive research on skin aging has developed, and important functions of collagen in skin have been revealed.

Effective ingredients promoting collagen synthesis and exhibiting wrinkle-reducing effects are known. For example, retinoic acid, transforming growth factor (TGF) [non-patent document 1], animal placenta-derived protein [Patent document 1], betulinic acid [Patent document 2], chlorella extract [ Patent Documents 3 and 4] are known as collagen synthesis promoting substances. However, the above-mentioned effective ingredients are limited in the use amount due to safety problems such as irritation and redness when applied to the skin, or have insufficient effect, so that the effect of improving the skin function by promoting the collagen synthesis of the skin can not be expected.

On the other hand, active oxygen introduced from the outside of the living body or generated in the living body causes many problems such as promoting aging of the living body, cancer, and the like. Therefore, the development and research of antioxidants that inhibit oxidation by active oxygen have been performed. Antioxidants are widely distributed in copper and plants. Many phenolic compounds, flavonoids, tocopherols, vitamin C and selenium are known in fruits and vegetables. However, the antioxidant substances present in nature can not be expected to have practically sufficient effects in skin application. Therefore, although synthetic antioxidants having excellent antioxidant ability and low cost are widely used, their use is restricted due to safety concerns such as human side effects.

In addition, inflammation is an immune response of a human body in response to a wound or disease, and oxidative stress such as ultraviolet rays, active oxygen, free radicals, etc. activate inflammatory factors and cause aging of various diseases and skin. The vasoactive polypeptide, kinin, plasmin and complement, have vasodilatory and contraction and chemotaxis effects, as well as lymphokines such as interleukin-6 (IL-6) Phosphorus and arachidonic acid are responsible for inflammation. Arachidonic acid is metabolized through inflammatory mediators such as prostaglandin and lukotrienes via two pathways: cyclooxygenase or lipooxygenase, mediating a variety of inflammatory responses.

On the other hand, in order to eliminate inflammation, elimination of inflammation source, reduction of vital reaction and symptoms is called anti-inflammatory. To date, substances used for antiinflammatory purposes include flutenamic acid, ibuprofen, benzydamine, indomethacin, and steroids, prednisolone, , Dexamethasone, and the like. It is also known that allantoin, azene, hydrocortisone and the like are effective for antiinflammation. However, these materials are limited in their use due to safety of skin, .

In addition, the epidermis located at the outermost part of the skin protects against various external physical, chemical and mechanical stimuli and protects against excessive divergence of body water through the skin. This protective function is possible by normally forming and maintaining the stratum corneum composed of keratinocytes. The keratinocyte is a cell formed by a stepwise change in morphology and function while a basal cell that continuously proliferates in the stratum basale moves to the stratum corneum, Forming cells are removed from the skin and the new keratinocytes from the epidermis's bottom layer repeat the process of epidermis differentiation or keratinization replacing its function. In this keratinization process, keratinocytes produce intercellular lipids such as natural moisturizing factors (NMF) and ceramides, cholesterol, and fatty acids, which act as barrier layers to the outside, As shown in Fig.

In addition, the skin texture, which is the shape of the skin surface, is characterized by a three-dimensional microstructure formed by fine lines and is significantly different according to age and site. The skin texture is divided into primary (about 20-100 μm), secondary (about 5-40 μm), and tertiary (about 0.5 μm) lines depending on the depth of the line. And the star formation is evident in the polygon and star formation that the lines meet [Non-Patent Document 2].

However, as the age increases or the skin becomes damaged, the network structure of the dense fine lines collapses and fine lines (secondary, tertiary, etc.) disappear and the primary lines become deeper and wrinkles are known to be produced Literature 3]. In other words, wrinkles, a typical sign of skin aging, are accumulations of minute changes that have already occurred over a long period of time.

Embryologically, all components of human skin are known to originate from ectoderm or mesodermal lobe. Epidermis, hair follicles, sebaceous glands and glands are originated from ectoderm, and melanocytes, nerves and special sensory receptors are derived from neuroepithelial cells. According to the developmental stage, the embryonic stem cells are repeatedly differentiated and become cells with the characteristics of each tissue function. After the embryo, a certain number of stem cells remain in the tissue. . The first is in the hair follicle. It is known to play an important role in the regeneration of the epidermis into cells before cell differentiation occurs, and plays an important role in hair regeneration and growth. The second is the basal layer of the epidermis. The stem cells found here play an important role in maintaining skin health by administering not only the epidermis but also the fibroblasts of the dermal layer. The stem cells here are relatively large in quantity and easy to obtain, making them widely used in the study of skin stem cells. The skin is constantly renewed, and stem cells present in the epithelium of the skin, that is, epidermal stem cells of the skin, are involved in the repair of the epithelium after injury [Non-Patent Document 4]. The epithelial stem cells of the skin are also referred to as skin stem cells. When the skin stem cells are activated, it is possible to treat skin wounds such as trauma, promote wound healing, have. Integrin β1 and integrin α6 are used as indicators of dermal stem cells, and maintenance of skin stem cells necessary for epithelial morphogenesis and differentiation has been reported to be regulated by p63 protein.

Dermal stem cells play an important role in maintaining the health and physiological and biochemical homeostasis of the skin. Stem cells present in the skin are also abnormally functioned due to the effect of aging, and thus various problems arise as the homeostasis of the skin is broken. Therefore, various phenomena of skin aging can be improved through the activation of stem cells [Non-Patent Document 5].

In addition, it is safe to use in the living body, stable in the active ingredient, and more effective than the substances having the effect of improving the existing skin, wrinkle, elasticity, antioxidant, antiinflammation, There is a desperate need for the development of a composition having an activity of promoting, antioxidation, anti-inflammation, moisturizing and skin-tone improving activity.

Japan Patent No. 8-231370 Japan Patent No. 8-208424 Japan Patent No. 9-40523 Japan Patent No. 10-36283

Cardinale G. et al, Adv. Enzymol., 41, p. 425, 1974 Journal of the European Academy of Dermatology and Venereology. 12: 103-114, 1999 The British journal of dermatology. 110: 129-138, 1984, Skin research and technology. 5: 189-194, 1999 Epidermal Stem Cells of the Skin, Cedric Blanpain, Elaine Fuchs, Annual Review of Cell and Developmental Biology, November 2006, Vol. 22, Pages 339-373 Human skin stem cells and the aging process, Catherine Niemann, Stem Cell Aging and Regenerative Medicine, November 2008, Pages 986-997

Thus, the present inventors have found that Eleutheroside D promotes collagen synthesis of fibroblasts in the skin, promotes proliferation of skin stem cells, promotes skin regeneration, improves wrinkles, promotes elasticity, The present invention has been accomplished by confirming the effect of improving the skin texture due to the improvement of the skin brightness as well as the skin moisturization improvement effect due to the increase of the fila green expression and the anti-inflammatory effect by suppressing the NO production.

Accordingly, an object of the present invention is to provide a composition for improving skin regeneration, wrinkle improvement, elasticity enhancement, antioxidation, antiinflammation, moisturizing and skin texture improvement comprising a compound represented by Chemical Formula 1 as an active ingredient:

[Chemical Formula 1]

Another object of the present invention is to provide a composition for promoting stem cell activity comprising a compound represented by the following formula (1) as an active ingredient and a cosmetic composition comprising the composition:

 [Chemical Formula 1]

Accordingly, the present inventors have found that Eleutheroside D promotes collagen synthesis of fibroblasts of the skin to promote skin regeneration, improve wrinkles, promote elasticity, exert antioxidative effects by eliminating free radicals, In addition to the skin moisturizing effect due to the increased expression of pilar green, it was confirmed that the improvement of the skin texture was improved by the improvement of the skin brightness, and the effect of promoting stem cell proliferation and stem cell maintenance Thereby completing the present invention.

Accordingly, an object of the present invention is to provide a composition for improving skin regeneration, wrinkle improvement, elasticity enhancement, antioxidation, antiinflammation, moisturizing and skin texture improvement comprising a compound represented by Chemical Formula 1 as an active ingredient:

[Chemical Formula 1]

Another object of the present invention is to provide a composition for promoting stem cell activity comprising a compound represented by the following formula (1) as an active ingredient and a cosmetic composition comprising the composition:

 [Chemical Formula 1]

The present invention relates to the use of eruteroside D for promoting collagen synthesis of fibroblasts of the skin to promote regeneration of the skin, to improve wrinkles, to improve elasticity, to eliminate free radicals, to exhibit antioxidative effects, And has an anti-inflammatory effect. It has an effect of improving skin moisturization due to an increase in filla green expression, and exhibits an effect of improving skin texture due to improvement in skin brightness, and thus can be used in pharmaceuticals, cosmetics or health foods. In addition, it promotes proliferation of dermal stem cells and exhibits a stem cell-retaining effect, and thus can be used as a composition for promoting stem cell activity and cosmetics containing the same.

Hereinafter, the configuration of the present invention will be described in detail.

To regenerate skin, improve wrinkles, improve elasticity, antioxidant, anti-inflammation, moisturizing and skin texture, it is necessary to apply high-active skin regeneration, wrinkle improvement, elasticity enhancement, antioxidant, anti-inflammation So that it can be absorbed through the skin and can stay for a sufficient time to exhibit skin regeneration, wrinkle improvement, elasticity enhancement, antioxidant, anti-inflammation, moisturizing and skin texture improving effect It is preferable to have a low volatility, to stably maintain the active ingredient on the composition or on the skin, to be easily formulated into medicines or cosmetics, and to be safe for the skin. However, the components satisfying all of the above-mentioned characteristics among the known components are not common. For example, some skin regeneration, wrinkle improvement, elasticity enhancement, antioxidant, antiinflammatory, moisturizing and skin tone improving ingredients can be used for skin regeneration, wrinkle improvement, elasticity enhancement, antioxidant, anti- It is excellent in improving activity, but it is difficult to apply to actual skin because of its ability to permeate through the skin and be absorbed. Other active ingredients have low hydrophilicity and are difficult to formulate into medicines or cosmetics. In addition, some skin regeneration, wrinkle improvement, elasticity enhancement, antioxidant, antiinflammatory, moisturizing and skin texture improving ingredients may be decomposed or converted into other compounds when exposed to heat, light or oxygen, In some cases, the effect disappears.

As can be seen in the following examples, the eruteroside D exhibits remarkably excellent collagen synthesis promoting effect, antioxidative effect, anti-inflammatory effect, pilar green expression effect and skin brightness improvement effect at low concentration, It can be used as an effective ingredient for medicines, cosmetics, and health food for improving wrinkles, improving elasticity, antioxidation, anti-inflammation, moisturizing and improving skin texture.

Accordingly, the present invention provides a pharmaceutical composition for skin regeneration, wrinkle improvement, elasticity enhancement, antioxidation, anti-inflammation, moisturizing and skin texture improvement comprising a compound represented by the following formula (1) as an active ingredient.

[Chemical Formula 1]

The compound of the above formula (I) is named [(1S, 3aR, 4S, 6aS) -tetrahydro-1H, 3H-furo [3,4-c] furan-1,4-diyl] bis (2,6- Furan [3,4-c] furan-1,4-dioxane (hereinafter referred to as " [(1S, 3aR, 4S, 6aS) -tetrahydro- bis-beta-D-glucopyranoside) and is also called eruteroside D (D).

The compound of formula (1) may be synthesized or a commercially available compound may be used.

The pharmaceutical composition for skin regeneration, wrinkle improvement, elasticity enhancement, antioxidation, anti-inflammation, moisturizing and skin texture improvement of the present invention may include a pharmaceutically acceptable salt of the compound of formula (1).

The pharmaceutically acceptable salt of the compound of Formula 1 may be an acid addition salt formed using an organic acid or an inorganic acid, and the organic acid may be, for example, formic acid, acetic acid, propionic acid, lactic acid, butyric acid, isobutyric acid, trifluoroacetic acid The organic acid may be selected from the group consisting of hydrochloric acid, hydrobromic acid, hydrochloric acid, hydrobromic acid, hydrobromic acid, hydrobromic acid, hydrobromic acid, hydrobromic acid, malic acid, maleic acid, malonic acid, fumaric acid, succinic acid, monoamide, glutamic acid, tartaric acid, oxalic acid, citric acid, glycolic acid, glucuronic acid, , Benzenesulfonic acid, p-toluenesulfonic acid and methanesulfonic acid-based salts, and the inorganic acid includes, for example, hydrochloric acid, bromic acid, sulfuric acid, phosphoric acid, nitric acid, carbonic acid and boric acid-based salts. Preferably in the hydrochloride or acetate form, more preferably in the hydrochloride form.

The above-mentioned acid addition salts may be obtained by a) directly mixing the compound of formula 1 and an acid, or b) dissolving and mixing one of them in a solvent or a water solvent, or c) Lt; RTI ID = 0.0 > acid < / RTI > in a solvent and mixing them.

In addition to the above, additionally saltable forms include, but are not limited to, the salts of gabapentin, pregabalin, nicotinate, adipate, hemimarate, cysteine, acetylcysteine, methionine, arginine, Aspartate and the like.

When the compound of Chemical Formula 1 of the present invention is used as a medicine, it may further contain one or more active ingredients showing the same or similar functions. For example, it may contain known skin regeneration, wrinkle improvement, elasticity enhancement, antioxidant, anti-inflammatory, moisturizing and skin tone improving ingredients. When the composition of the present invention contains additional ingredients for skin regeneration, wrinkle improvement, elasticity enhancement, antioxidation, anti-inflammation, moisturizing and skin texture improvement, the skin regeneration, wrinkle improvement, elasticity enhancement, antioxidant, anti- Can be further enhanced. When the above ingredients are added, skin safety, easiness of formulation, and stability of effective ingredients can be considered according to the combined use. In one embodiment of the present invention, the composition is a skin regeneration component known in the art comprising retinoic acid, TGF, protein from animal placenta, betulinic acid and chlorella extract, antioxidant components known in the art, such as tocopherol, selenium , Vitamin C and phenolic compounds, whitening ingredients known in the art, substances inhibiting the activity of tyrosinase enzymes such as kojic acid and arbutin, hydroquinone, vitamin C (L- Ascorbic acid, derivatives thereof, and various plant extracts. The additional component may be included in an amount of 0.0001 to 10% by weight based on the total weight of the composition, and the content range may be adjusted according to requirements such as skin safety, ease of formulation of the compound of Formula 1 .

In addition, the pharmaceutical composition for skin regeneration, wrinkle improvement, elasticity enhancement, antioxidation, anti-inflammation, moisturizing and skin texture improvement of the present invention may further comprise a pharmaceutically acceptable carrier.

Pharmaceutically acceptable carriers may contain a variety of ingredients such as buffer, injectable sterile water, normal saline or phosphate buffered saline, sucrose, histidine, salts and polysorbates.

The pharmaceutical composition of the present invention can be administered orally or parenterally, and can be administered in the form of a general pharmaceutical preparation, for example, various forms of oral and parenteral administration at the time of clinical administration. In the case of formulation, , An extender, a binder, a wetting agent, a disintegrant, a surfactant, and the like.

Solid formulations for oral administration include tablets, pills, powders, granules, capsules and the like, which may be prepared by mixing the pharmaceutical composition of the present invention with at least one excipient such as starch, calcium carbonate, Sucrose, lactose, gelatin, and the like.

In addition to simple excipients, lubricants such as magnesium stearate talc are also used. Examples of liquid formulations for oral administration include suspensions, solutions, emulsions, syrups and the like. Various excipients such as wetting agents, sweeteners, fragrances, preservatives and the like may be included in addition to water and liquid paraffin, which are simple diluents commonly used.

Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. Propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like can be used as the non-aqueous solvent and suspension agent. Examples of the suppository base include witepsol, macrogol, tween 61, cacao butter, laurin, glycerogelatin and the like.

In the present invention, the term " skin regeneration effect " refers to restoration of skin tissue against damage caused by external or internal causes of the skin. Damage due to external causes may include ultraviolet rays, external contaminants, wound, trauma, etc. The damage caused by the internal causes may be stress.

In the present invention, the term " wrinkle-reducing effect " refers to inhibiting or inhibiting the generation of wrinkles on the skin, or alleviating already-generated wrinkles.

In the present invention, the 'elasticity-enhancing effect' refers to an increase in elasticity to the skin, which suppresses or inhibits the loss of elasticity of the skin, or alleviates the already-reduced elasticity.

In the present invention, the term 'antioxidative effect' refers to the action of free radicals or reactive oxygen species (ROS), which are highly reactive according to oxidative stress caused by intracellular metabolism or ultraviolet rays, Refers to inhibition of oxidation, and includes removal of free radical or reactive oxygen species, thereby reducing damage to the cells.

In the present invention, the term 'anti-inflammatory effect' refers to inhibition of inflammation. The inflammation is one of defensive responses of biological tissues to certain stimuli, and involves three types of tissue degeneration, circulatory disorder, exudate, and tissue proliferation Complex lesions. More specifically, inflammation is part of congenital immunity and, like in other animals, human congenital immunity recognizes a pattern of cell surfaces that are specifically present in a pathogen. Phagocytes recognize cells with such surfaces as non-magnetic and attack pathogens. If pathogens break through the physical barriers of the body, an inflammatory reaction occurs. Inflammation is a nonspecific defense that creates hostile environments for microorganisms entering the wound. In the inflammatory response, white blood cells that are responsible for the early stage of immune response, when wounded or infected, enter the body to express cytokines. Therefore, the expression level of intracellular cytokine is an index of inflammatory response activation. Examples of skin diseases associated with inflammation include atopic dermatitis, psoriasis, radiation, chemical substances, inflammatory diseases caused by burns, acid burns, vesicular dermatosis, visceral type diseases, itching due to allergies, seborrheic eczema, rose acne, Inflammatory hair loss such as pemphigus vulgaris, polymorphous exudative erythema, erythema nodosum, erythematosus, pharyngitis, alopecia areata, skin T-cell lymphoma, and the like.

In the present invention, the term 'moisturizing effect' refers to inhibiting or reducing the reduction of moisture in the skin, or increasing the moisture content of the skin to smooth and smooth the surface of the skin.

In the present invention, the term " skin texture improving effect " refers to smoothing the skin surface, imparting gloss, and brightening the skin tone by inhibiting or inhibiting roughness of the skin surface due to aging, stress, .

The pharmaceutical composition of the present invention can provide a desirable skin regeneration effect, wrinkle improvement effect, elasticity enhancement effect, antioxidative effect, anti-inflammatory effect, moisturizing effect and skin texture improving effect when the compound of Chemical Formula 1 is contained in an effective amount . In the present invention, the term "effective amount" refers to an amount effective for promoting the regeneration of damaged skin, improving wrinkles, improving elasticity, inhibiting or alleviating the oxidation of cells, suppressing inflammation, Quot; means the amount of a compound capable of exhibiting a moisturizing effect. An effective amount of the compound of formula (1) contained in the composition of the present invention will vary depending on the form in which the composition is commercialized, how the compound is applied to the skin, and the time on the skin. For example, when the composition is commercialized as a pharmaceutical product, the compound of Formula 1 may be contained at a higher concentration than that of a cosmetic product that is routinely applied to skin. Accordingly, the daily dosage is 0.1 to 100 mg / kg, preferably 30 to 80 mg / kg, more preferably 50 to 60 mg / kg, based on the amount of the compound of formula (1) 6 times a day.

The pharmaceutical composition of the present invention can be used alone or in combination with methods using surgery, radiation therapy, hormone therapy, chemotherapy, and biological response modifiers.

The present invention can also be provided as a formulation for external skin preparation for skin regeneration, wrinkle improvement, elasticity enhancement, antioxidation, anti-inflammation, moisturizing and skin texture improvement comprising the compound of formula 1 as an active ingredient.

When the compound of formula (1) is used as an external preparation for skin, it may further comprise at least one selected from the group consisting of fatty substances, organic solvents, solubilizers, thickeners and gelling agents, softeners, antioxidants, suspending agents, stabilizers, foaming agents, , Water, ionic or nonionic emulsifiers, fillers, sequestering agents and chelating agents, preservatives, vitamins, blocking agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or external preparations for skin And any other ingredients used in the skin sciences. The components can also be introduced in amounts commonly used in the field of dermatology.

When the compound of Formula 1 is provided as an external preparation for skin, it may have a formulation such as, but not limited to, ointments, patches, gels, creams or sprays.

The present invention can also be provided as a cosmetic formulation for improving skin regeneration, wrinkle improvement, elasticity enhancement, antioxidation, anti-inflammation, moisturizing and skin texture improvement comprising the compound of formula (1) as an active ingredient.

When the compound of Chemical Formula 1 is used as a cosmetic, the cosmetic product containing the compound of Chemical Formula 1 as an active ingredient may be prepared in the form of a general emulsified formulation and a solubilized formulation. For example, creams, essences, cosmetic creams, sprays, gels, packs, sunscreens, make-up bases, liquids such as lotions such as lotion, facial lotion, body lotion, A powder, a cleansing lotion, a makeup removing agent such as a cleansing oil, a cleaning agent such as a cleansing foam, a soap, a body wash and the like.

The cosmetic composition may further contain, in addition to the compound of Formula 1, a lipid, an organic solvent, a solubilizing agent, a thickening agent and a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizer, a foaming agent, a fragrance, , Ionic or nonionic emulsifiers, fillers, sequestering and chelating agents, preservatives, vitamins, barrier agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or cosmetics And may contain adjuvants commonly used in the cosmetics field, such as any of the other ingredients.

In the case of a wash-off type cosmetic such as a make-up removing agent, a detergent, etc. in which the active ingredient remains on the skin in a short period of time when the compound of formula (1) is produced into a cosmetic product, . On the other hand, in the case of leave-on type cosmetics, such as lotion, cream, essence, etc., in which the active ingredient remains on the skin for a long period of time, It may be included. In one embodiment of the present invention, although not limited thereto, the composition may contain 0.0001 wt% to 10 wt% (preferably 0.0001 wt% to 1 wt%) of the compound of Formula 1 based on the total weight of the composition . When the composition of the present invention contains less than 0.0001% by weight of the compound of Formula 1, sufficient skin regeneration, wrinkle improvement, elasticity enhancement, antioxidative, antiinflammatory, moisturizing and skin texture improving effects can not be expected, It is intended to prevent an undesired reaction such as allergy or a problem of skin safety.

The present invention also relates to a health food for skin regeneration, wrinkle improvement, elasticity enhancement, antioxidation, anti-inflammation, moisturizing and skin texture improvement comprising the compound of formula (1).

The term 'health food' as used herein means a food prepared by adding the compound of formula (1) to food materials such as beverage, tea, spice, gum and confectionery, or by encapsulation, powdering or suspension, But it has the advantage that there are no side effects that can occur when a drug is used for a long period of time using a food as a raw material.

Since the health food of the present invention thus obtained can be routinely ingested, it is very useful because high skin regeneration, wrinkle improvement, elasticity enhancement, antioxidation, anti-inflammation, moisturizing and skin texture improving effect can be expected.

When the compound of Chemical Formula 1 is used as a food additive, the compound of Chemical Formula 1 may be added as it is or may be used together with other food or food ingredients, and may be suitably used according to a conventional method. The amount of the active ingredient to be mixed can be suitably determined according to its intended use (prevention, health or therapeutic treatment). Generally, the composition of the present invention is added in an amount of not more than 15 parts by weight, preferably not more than 10 parts by weight, based on the raw material, when the food or beverage is produced. However, in the case of long-term consumption intended for health and hygiene purposes or for health control purposes, the amount may be less than the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount exceeding the above range .

There is no particular limitation on the kind of the food. Examples of the food to which the above substance can be added include dairy products including meat, sausage, bread, chocolate, candy, snack, confectionery, pizza, ramen, other noodles, gums, ice cream, various soups, drinks, tea, Alcoholic beverages, and vitamin complexes, all of which include healthy foods in a conventional sense.

The health beverage composition of the present invention may contain various flavors or natural carbohydrates as an additional ingredient such as ordinary beverages. Such natural carbohydrates are monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, and polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol and erythritol. Examples of sweeteners include natural sweeteners such as tau martin and stevia extract, synthetic sweeteners such as saccharin and aspartame, and the like. The ratio of the natural carbohydrate is generally about 0.01 to 0.04 g, preferably about 0.02 to 0.03 g per 100 mL of the composition of the present invention.

In addition to the above, the health food of the present invention may contain various nutrients, vitamins, electrolytes, flavors, colorants, pectic acids and salts thereof, alginic acid and its salts, organic acids, protective colloid thickeners, pH adjusting agents, stabilizers, preservatives, , A carbonating agent used in carbonated drinks, and the like. In addition, the health food of the present invention may contain flesh for the production of natural fruit juice, fruit juice drink and vegetable drink. These components may be used independently or in combination. The proportion of such additives is not critical, but is generally selected in the range of 0.01 to 0.1 parts by weight per 100 parts by weight of the composition of the present invention.

The present invention also provides a composition for promoting stem cell activity comprising the compound of Formula 1 as an active ingredient.

The term 'stem cell' in the present invention is a cell capable of cell division by itself and capable of differentiating into a very specific type of specific cell type. The type of such stem cells is not particularly limited, and in one embodiment, the stem cells may be dermal stem cells. The term 'dermal stem cells' refers to stem cells that can be differentiated into cells constituting the skin (epidermis, dermis and subcutaneous fat layer). The cells that make up the skin include keratinocytes, melanocytes, and fibroblasts (mainly responsible for biosynthesis of collagen and elastin) present in the epidermis.

The kind of the skin stem cell is not particularly limited. The dermal stem cells used in the present invention can be used irrespective of where they originate from. For example, dermal stem cells may be obtained from a known source of dermal stem cells, e. G., From the basal layer of hair follicles or epidermis, and the animal to be harvested may be a mammal. In one embodiment, the mammal may include, but is not limited to, a human, a mouse, a rat, a guinea pig, a rabbit, a monkey, a pig, a horse, a cattle, a sheep, Preferably the mammal can be human. Such methods of obtaining dermal stem cells from dermal stem cell sources are well known in the art.

In the present invention, 'stem cell activity promoting effect' refers to a stem cell proliferation promoting effect and / or a stem cell maintaining effect.

Dermal stem cells play an important role in maintaining the health and physiological and biochemical homeostasis of the skin. Stem cells present in the skin are also abnormally functioned due to the effect of aging, and thus various problems arise as the homeostasis of the skin is broken. Non-Patent Document 5: In the present invention, skin regeneration, wrinkle improvement, elasticity enhancement, antioxidation, anti-inflammation, moisturizing and skin texture improving effect can be improved through the activity of stem cells Respectively.

Accordingly, the present invention can provide a cosmetic composition comprising the composition for promoting stem cell activity. These cosmetic formulations are the same as those described in the description of cosmetic formulations including the compound of formula (1).

Hereinafter, the present invention will be described in detail with reference to examples. However, the following examples are illustrative of the present invention, and the present invention is not limited to the following examples.

Reference Example  One: Eruteroside  D ( Eleutheroside  D) Material information

[Chemical Formula 1]

CAS No .: 79484-75-6

Where to buy: Sichuan Hongjie Import & Export CO., LTD

Reference Example  2: Serum-free Under medium conditions  Cultivation of dermal stem cells

Human epidermal stem cells purchased from Cellntec were added to 48-well plates (6 × 10 ³ cells / well), supplemented with BPE (bovine pituitary extract) similar to fetal bovine serum And cultured for 24 hours at 5% CO ₂ and 37 ° C using CNT-57 medium (Cellntec). Thereafter, the culture medium was removed through a suction tube, and the medium was removed using a PBS solution (GibcoBRL). 20 / / mL of the compound of formula (2) was treated with CNT-57 medium containing no BPE, ₂ and 37 < 0 > C for 72 hours.

Experimental Example  One: CCK Promoting the proliferation of skin stem cells through the -8 evaluation method

The skin stem cells cultured in Reference Example 2 were evaluated for CCK-8 (Cell counting kit-8). The CCK-8 evaluation is an indirect method of measuring the density of living cells by measuring the absorbance of Formazan, a dehydrogenase in the intracellular electron transport system, which is formed by the decomposition of tetrazolium salt.

Cells were spectrophotometrically measured by treating CCK-8 solution (treated with 1/10 of the medium volume) at 37 ° C for 2 hours and then measuring the absorbance at 450 nm. The proliferation rate (%) of the compound of the formula (1) was determined from the measured absorbance value according to the following equation (1) with respect to the control group (CNT-57 medium supplemented with BPE) .

[Equation 1]

Growth rate (%) = (absorbance of sample-treated group / absorbance of control group) x 100

Promoting the proliferation of dermal stem cells Additive sample density Cell proliferation (%) versus control The compound of formula (1) 20 / / mL 103.5% Control group - 100%

As can be seen from the results of Table 1, the dermal stem cells in the medium treated with the compound of formula (I) of the present invention showed an excellent proliferation promoting effect.

Experimental Example  2: Stem Cellularity of Dermal Stem Cells stemness ) Confirm maintenance effect

The expression level of p63 was evaluated in order to confirm the stem cell maintenance effect of the dermal stem cells cultured in Reference Example 2 above. p63 is an intracellular transcription factor and is well known as a maintenance marker for dermal stem cells. Cells were isolated and cDNA was synthesized. Then, real-PCR was performed with Taqman staining solution to measure the expression level of p63. The concentration of RNA in this experiment was normalized to S16 ribosomal RNA.

The experimental results are shown in Table 2 below. In Table 2 below, the numerical value of the increase in p63 is a multiple of the control group (CNT-57 medium supplemented with BPE).

Stem cell (stem cell) maintenance effect (number of repeats = 3) Additive sample density p63 Incremental multiple (times) The compound of formula (1) 20 / / mL 3.8 Control group - 1.0

As can be seen from the results of Table 2, the medium condition treated with the compound of Chemical Formula 1 of the present invention promotes the expression of p63 in dermal stem cells, and thus has excellent stem cell maintenance effect.

Example  1: Promoting collagen synthesis

The compound of Chemical Formula 1 was added to the culture medium of human fibroblasts to examine the effect of promoting collagen synthesis at the cellular level. The biocompatible collagen was quantitated using a PICP EIA kit (Procollagen Type I C-Peptide Enzyme Immunoassay Kit).

(1 x 10 < ⁴ > cells / cm < ² >) together with vitamin C and a control (no supplement) After culturing for 1 day, the culture broth was taken and the degree of collagen biosynthesis at each concentration was measured at 450 nm using a spectrophotometer with PICP EIA Kit. The collagen biosynthesis performance was calculated by the relative performance relative to the control group and the results are summarized in Table 3 below.

Promotion of collagen synthesis by concentration (number of repeats = 3) Additive sample Application concentration (/ / ml) Increase in collagen synthesis (%) compared to control The compound of formula (1) 10 [mu] g / ml 46.3% The compound of formula (1) 20 ug / ml 58.0% Vitamin C 50 ug / ml 30.6%

As a result, as shown in the results of Table 3, the compound of Chemical Formula (1) of the present invention has excellent collagen synthesis ability against human-derived fibroblasts, and has a lower concentration than that of vitamin C, It is possible to obtain a better collagen synthesis effect.

Example  2: Antioxidant effect - Free radical  Erasure rate

The free radical scavenging activity was measured to confirm the antioxidative activity of the compound of formula (1). Free radical scavenging activity was measured using DPPH. DPPH was purchased from Sigma Co. (Sigma Co., Ltd, USA) and used. First, a standard DPPH ethanol solution of 1.5 mM (0.06 mg / ml) was prepared. Then, ethanol was added to ascorbic acid, which is an antioxidant, as a standard compound and a compound of formula (1) to prepare samples at concentrations of 50 / / . Then, the sample and standard DPPH solution were added at the same ratio, stirred well, reacted at 37 ° C for 30 minutes, and absorbance was measured at 520 nm. At this time, ethanol was added instead of the sample to give a control group. IC ₅₀ , which is half maximal inhibitory concentration (inhibition value), was determined for the free radical scavenging ability, and the results are shown in Table 4 below. IC ₅₀ is a common method of expressing the free radical scavenging ability as a concentration of ascorbic acid and the compound of Formula 1 required to remove 50% of the free radicals of the no-added control group.

Free radical scavenging rate (IC ₅₀ ) sample Free radical scavenging rate (ug / ml) Ascorbic acid 5.2 The compound of formula (1) 3.6

As can be seen from the results of Table 4, the compound of formula (1) shows high antioxidative effect as compared with ascorbic acid which is known antioxidant.

Example  3: Anti-inflammatory effect - NO  Production inhibitory effect

In order to confirm the antiinflammatory effect and the skin trouble relieving effect of the compound of Chemical Formula 1, nitric oxide (NO) formation inhibition experiment was performed by GRIESS using RAW264.7 cell line (ATCC number: CRL-2278).

Specifically, RAW264.7 cells, macrophages of mice, were subcultured several times, placed in 24-well plates at 3 × 10 ⁵ per well, and cultured for 24 hours. Subsequently, the compound of Chemical Formula 1 was diluted with the concentrations shown in Table 5 below and replaced with a cell culture medium containing the compound. At this time, 20 μg / ml of L-NMMA (L-NG-Monomethylarginine), which is an inhibitor of NO production, was treated with positive control and cultured for 30 minutes. Lipopolysaccharide (LPS) Lt; / RTI > 100 μl of the supernatant was transferred to a 96-well plate, and 100 μl of the GRIESS solution was added thereto at room temperature for 10 minutes. The absorbance at 540 nm was measured to determine the NO inhibitory effect of Compound 1, Table 5 shows the results.

NO generation inhibitory effect sample NO production inhibition rate (%) L-NMMA (positive control) 41.53 Compound (1) (0.1%) 32.82 Compound (1) (1) 45.02

As can be seen from the results in Table 5, the compound of Chemical Formula 1 has the same inhibitory effect on NO production as that of L-NMMA, which is a known anti-inflammatory substance.

Example  4: Filaggrin's  Promoting effect of expression

The cells were incubated for 1 day in a culture medium supplemented with human keratinocyte primary HaCaT cells at a concentration of 5 μg / ml and 10 μg / ml, respectively. Cellular RNA was isolated, cDNA was synthesized, and Taqman staining solution Real-time PCR was performed. The RNA concentration was normalized to S16 ribosomal RNA.

Effect of expression of pillar green depending on concentration (number of repeats = 3) Additive sample Increase in pillar green compared to control Control group (no addition) 1.00 The compound of formula (1) (5 [mu] g / ml) 31.2 The compound of formula (1) (10 [mu] g / ml) 48.0

As can be seen from the results of Table 6, the compound of Chemical Formula 1 has the ability to promote pilar green expression of human horny cell primary HaCaT cells.

Example  5: exfoliation of the keratin turn - over ) For improvement Efficacy

About the nutritional cream of the following formulation example 2, the effect of the improvement of the exfoliation of the skin in the healthy twenties to 50 women was tested as follows.

Twenty of 20 women aged 20 to 50 years were given a 1.5% solution of DHA (Dihydroxyacetone, Sigma Aldrich, USA) for 8 hours, followed by deposition. The preparation cream was applied twice daily to the test site. After 4 days of application, 8 days after application, 12 days after application, photographs were taken using instrument Chromameter CR-400 (Minolta, Japan) and skin lightness of DSLR and DSLR. The measured values were evaluated by three mean values excluding the maximum value and the minimum value. The higher the improvement in the skin brightness of the deposition site, the better the improvement of the exfoliation.

The results are shown in Table 7 below.

Skin brightness improvement effect division Four days after application Eight days after application After 12 days of application Improvement rate (%) 1.52 5.02 10.5

As shown in Table 7, when the nutritional cream according to the present invention was used, it was found to have an effect of improving the exfoliation of the skin.

Formulation example  1: Preparation of pharmaceutical preparations

1. Preparation of tablets

0.2 mg of the compound of formula (1)

100 mg of corn starch

100 mg of milk

Stearic acid  Magnesium 2 mg

After mixing the above components, tablets were prepared by tableting according to a conventional method for producing tablets.

Formulation example  2: Manufacture of cosmetics

1. Manufacture of nutritional cream

A nutritional cream containing the compound of formula (1) as an active ingredient was prepared according to a conventional method, as shown below.

0.2% by weight of the compound of formula (1)

Beta-1,3-glucan 5.0 wt%

Wax 10.0 wt%

Polysorbate 60 1.5 wt%

≪ tb > < tb > < tb >

0.5% by weight of sorbitan sesquioleate

Liquid paraffin 10.0 wt%

Squalane 5.0 wt%

Caprylic / capric triglyceride 5.0 wt%

Glycerin 5.0 wt%

3.0% by weight of butylene glycol

3.0% by weight of propylene glycol

0.2% by weight triethanolamine

Preservative 0.05 wt%

0.05% by weight of pigment

0.05% by weight fragrance

Purified water to 100%

Formulation example  3: Topical  Produce

1. Manufacture of ointment

An ointment containing the compound of formula (I) as an active ingredient was prepared according to a conventional method, as shown below.

0.5% by weight of the compound of formula (1)

Beta-1,3-glucan 10.0 wt%

Wax 10.0 wt%

Polysorbate 60 5.0 wt%

≪ tb > < tb > < tb >

0.5% by weight of sorbitan sesquioleate

Vaseline 5.0 wt%

Liquid paraffin 10.0 wt%

Squalane 5.0 wt%

SHARE BUTTER 3.0 wt%

Caprylic / capric triglyceride 5.0 wt%

Glycerin 10.0 wt%

Propylene glycol 10.2 wt%

0.2% by weight triethanolamine

Preservative 0.05 wt%

0.05% by weight of pigment

0.05% by weight fragrance

Purified water to 100%

Claims (15)

A skin external preparation for skin regeneration and wrinkle improvement comprising a compound represented by the following formula (1) as an active ingredient:
[Chemical Formula 1]

A cosmetic composition for skin regeneration and wrinkle improvement comprising a compound represented by the following formula (1) as an active ingredient:
[Chemical Formula 1]

An external skin preparation for skin elasticity enhancement comprising a compound represented by the following formula (1) as an active ingredient:
[Chemical Formula 1]

A cosmetic composition for enhancing skin elasticity comprising a compound represented by the following formula (1) as an active ingredient:
[Chemical Formula 1]

An external skin preparation for antioxidation comprising a compound represented by the following formula (1) as an active ingredient:
[Chemical Formula 1]

A cosmetic composition for antioxidation comprising a compound represented by the following formula (1) as an active ingredient:
[Chemical Formula 1]

An antiinflammatory skin external preparation comprising a compound represented by the following formula (1) as an active ingredient:
[Chemical Formula 1]

An anti-inflammatory cosmetic composition comprising a compound represented by the following formula (1) as an active ingredient:
[Chemical Formula 1]

A skin external preparation for skin moisturizing comprising a compound represented by the following formula (1) as an active ingredient:
[Chemical Formula 1]

A cosmetic composition for skin moisturizing comprising a compound represented by the following formula (1) as an active ingredient:
[Chemical Formula 1]

An external skin preparation for improving skin texture comprising a compound represented by the following formula (1) as an active ingredient:
[Chemical Formula 1]

A cosmetic composition for improving skin texture comprising a compound represented by the following formula (1) as an active ingredient:
[Chemical Formula 1]

A composition for stimulating stem cell activity comprising a compound represented by the following formula (1) as an active ingredient:
[Chemical Formula 1]

14. The method of claim 13,
Wherein said stem cell activity is promoted stem cell proliferation or stem cell maintenance.
A cosmetic composition comprising the composition for stimulating stem cell activity according to claim 13.