KR102577528B1 - Composition for improving skin - Google Patents

Abstract

The present invention relates to a composition for improving skin. The pea extract according to the present invention can be used in the production of medicines, cosmetics or foods for skin regeneration through its effect in promoting the proliferation of skin stem cells. In addition, it promotes the proliferation of skin stem cells and has the effect of maintaining stem cell properties, so it can be used to manufacture cosmetics for promoting stem cell activity.

Description

Composition for improving skin {Composition for improving skin}

The present invention relates to a composition for improving skin that has the effect of promoting skin regeneration and stem cell activity.

Embryologically, all components of human skin are known to be derived from ectoderm or mesoderm. The epidermis, hair follicles, sebaceous glands, and sweat glands originate from the ectoderm, while melanocytes, nerves, and special sensory receptors originate from the neuroectoderm. Depending on the developmental period, embryonic stem cells undergo repeated differentiation to become cells with characteristics suited to each tissue function, and even after becoming an adult, a certain number of stem cells remain in the tissue. In the skin, stem cells mainly exist in two locations. . The first exists in hair follicles. This is a cell before cell differentiation occurs and is known to play an important role in the regeneration of the epidermis, and also plays an important role in hair regeneration and growth. The second is the basal layer of epidermis. It was revealed that the stem cells located here play an important role in protecting skin health by controlling not only the epidermis but also the fibroblasts of the dermal layer. Stem cells here have the advantage of being relatively large in quantity and easy to obtain, so they are widely used in skin stem cell research. The skin is continuously regenerated, and the stem cells present in the epithelium of the skin, that is, the epidermal stem cells of the skin, are involved in the repair of the epithelium after injury [Non-patent Document 7]. The epithelial stem cells of the skin are also called skin stem cells, and when the skin stem cells are activated, skin wounds such as trauma can be treated or wound healing can be promoted, and skin wrinkles can also be alleviated. there is. Integrin β1 and integrin α6 are used as indicators of skin stem cells, and it is reported that the maintenance of skin stem cells required for epithelial morphogenesis and differentiation is regulated by the p63 protein.

Skin stem cells play an important role in maintaining skin health and physiological and biochemical homeostasis. Stem cells present in the skin also malfunction due to the effects of aging, and as a result, the homeostasis of the skin is broken, causing various problems. Therefore, various phenomena of skin aging may be improved through the activity of these stem cells [Non-patent Document 8].

In addition, there is an urgent need to develop ingredients that are safe for the living body, have stable active ingredients, and, above all, have skin regenerative activity that is more effective than existing skin regenerative substances.

Meanwhile, small beans ( Canavalia gladiata ) is an annual vine plant belonging to the Rosaceae legume family. In Oriental medicine, it is called dodu (刀豆) and its fruit looks like a pea, so it is called a pea. There is no hair on the stem, and each bean grain is 2 to 3 cm long and weighs 1.5 to 3 g, making it the largest among bean types. It is native to tropical Asia and is cultivated for food. The color of black beans is red or white. The red variety is mainly used for pods, and the white variety is mainly used for beans, but all parts are edible. All parts, including seeds, skin, leaves, stems, and roots, can be used as food materials. You can.

Peas are known to contain various bioactive substances such as the hydrolytic enzyme urease, hemagglutinin, canavalin, linolenic acid, saponin, choline, resicin, dietary fiber, and protein. . In addition, it has an excellent effect in eliminating inflammation and increases the body's immunity, so it is effective in various boils and purulent diseases. It warms the stomach and is effective in asthma, phlegm, arthritis, and renal lumbar pain. It strengthens the kidneys and has an emollient effect. It is known to treat a variety of conditions such as arthritis, constipation, and obesity. It is also known to be very effective in hemorrhoids, sinusitis, otitis media, gastritis, and colitis, and is attracting attention as a functional food and pharmaceutical material.

Domestic patent documents related to soybeans include Patent No. 0350911, “Antibacterial activity extract isolated from soybeans and method for producing the same,” Patent No. 0394584, “Soymilk with added soybeans and method for producing the same,” and Patent No. 0324132. Cheonggukjang and its manufacturing method using black beans", Patent Application No. 2002-0055538 "Skin cosmetics using mixed extracts of black beans and golden pine", Patent Application No. 2002-0056697 "Development of an anti-nematode preparation using black beans", Patent Application No. These include No. 2001-0031264, “Development of immune function enhancing and antioxidant functional foods using peas, etc.,” and Patent Application No. 2003-0005294, “Composition for the treatment of allergies and inflammation.”

However, there is no research on stem cells in soybeans.

1. Japanese Patent Laid-open No. 8-231370 2. Japanese Patent Laid-open No. 8-208424 3. Japanese Patent Laid-open No. 9-40523 4. Japanese Patent Laid-Open No. 10-36283

1. Cardinale G. et al, Adv. Enzymol., 41, p. 425, 1974 2. Feingold et al., J. Invest. Dermatol., 110, pp 368-375, 1998. 3. Feingold et al., J. Invest. Dermatol., 115, pp 353-360, 2000. 4. Journal of the European Academy of Dermatology and Venereology. 12:103-114, 1999 5. The British journal of dermatology. 110: 129-138, 1984 6. Skin research and technology.5:189-194, 1999 7. Epidermal Stem Cells of the Skin, Cedric Blanpain, Elaine Fuchs, Annual Review of Cell and Developmental Biology, November 2006, Vol. 22, Pages 339-373 8. Human skin stem cells and the aging process, Catherin Niemann, Stem Cell Aging and Regenerative Medicine, November 2008, Pages 986-997

Accordingly, the present inventors completed the present invention by confirming the effect of pea extract on promoting stem cell activity, as it promotes skin regeneration by promoting the proliferation of skin stem cells and maintains stem cell properties.

Accordingly, the purpose of the present invention is to provide a composition for skin regeneration containing pea extract as an active ingredient.

In addition, another object of the present invention is to provide a composition for promoting stem cell activity containing pea extract as an active ingredient.

As a means to solve the above problems, the present invention provides a composition for skin regeneration containing a pea extract as an active ingredient for manufacturing medicine, cosmetics or food.

As another means to solve the above problem, the present invention provides a composition for promoting stem cell activity containing a pea extract as an active ingredient for the production of cosmetic formulations.

The pea extract according to the present invention has the effect of promoting skin regeneration by promoting the proliferation of skin stem cells, and can be used in medicine, cosmetics, or food production.

In addition, soybean extract promotes the proliferation of skin stem cells and has the effect of maintaining stem cell properties, so it can be used in the manufacture of cosmetic formulations for promoting stem cell activity.

Hereinafter, the configuration of the present invention will be described in detail.

In order for skin regeneration ingredients to exhibit excellent effects when applied to actual skin, they must exhibit highly active skin regeneration activity at low concentrations, have an excellent ability to penetrate and be absorbed through the skin, and be volatile enough to remain for a sufficient period of time to exhibit a skin regeneration effect. It is desirable that this is low, that the active ingredient is stably maintained in the composition or on the skin, that it is easy to manufacture into medicine, cosmetics, or food, and that it is safe on the skin. However, among known components, components that satisfy all of the above characteristics are rare. For example, some skin regenerative ingredients have excellent skin regenerative activity even at low concentrations when tested in vitro, but their ability to penetrate and absorb through the skin is poor, making them difficult to apply to actual skin. Other active ingredients have low hydrophilicity, making them difficult to formulate into medicines, cosmetics, or foods. Additionally, some skin rejuvenating ingredients may lose their effectiveness before application to the skin because the active ingredients are decomposed or transformed into other compounds when exposed to heat, light, or oxygen.

As can be seen in the examples below, the pea extract exhibits an excellent effect of promoting skin stem cell proliferation at low concentrations, and can therefore be used as an active ingredient in medicines, cosmetics, or food compositions for skin regeneration.

Accordingly, the present invention provides a composition for skin regeneration containing pea extract as an active ingredient.

The pea extract can be extracted by a method known in the art, and the method is not particularly limited. Alternatively, commercially available extracts can be used.

Preferably, the black bean extract may be an extract obtained by extracting black beans with water and/or an organic solvent, and the organic solvent may be a polar organic solvent, a non-polar organic solvent, or a mixed solvent thereof. The polar organic solvent may be a lower alcohol having 1 to 5 carbon atoms, ethyl acetate, or acetone, and the non-polar organic solvent may be ether, chloroform, benzene, hexane, or dichloromethane. For example, the lower alcohol having 1 to 5 carbon atoms may be methanol, ethanol, propanol, butanol, or isopropanol.

In one embodiment, the pea extract may include a fraction obtained by fractionating the primary extract extracted using the above-described extraction solvent using an extraction solvent of different polarity. For example, the soybean extract may be a fraction extracted with an alcohol having 1 to 5 carbon atoms and then fractionated again with a solvent of different polarity such as ether, benzene, or hexane. Two or more types of solvents can be used during the fractionation, and extracts for each solvent can be prepared by using them sequentially or by mixing them depending on the polarity of the solvents.

In the present invention, the prepared extract or the fraction obtained by performing the fractionation process can be filtered, concentrated, or dried to remove the solvent, and the filtration, concentration, and drying can all be performed. Specifically, the filtration can be performed using filter paper or a vacuum filter, the concentration can be performed under reduced pressure using a vacuum concentrator, etc., and the drying can be performed using a freeze-drying method.

In addition, the prepared extract or the fraction obtained by performing the fractionation process may be subjected to silica gel column chromatography, thin layer chromatography, or high performance liquid chromatography. Additional purified fractions can be obtained by purification using various chromatographies.

The compositions of the present invention can be used for preparing pharmaceutical formulations.

The pharmaceutical formulation may be in the form of a solution, suspension or emulsion in oil or aqueous medium, or in the form of extracts, powders, granules, tablets or capsules.

In addition, the composition may further contain one or more active ingredients that exhibit the same or similar functions. For example, it may contain known skin regeneration ingredients. The skin regeneration effect of the composition of the present invention can be further improved by including additional skin regeneration ingredients. When adding the above ingredients, skin safety due to combined use, ease of formulation, and stability of the active ingredients can be taken into consideration. In one embodiment of the present invention, the composition includes skin regeneration ingredients known in the art, such as retinoic acid, TGF, protein derived from animal placenta, betulinic acid and chlorella extract, and antioxidant ingredients known in the art, such as tocopherol and selenium. , vitamin C and phenolic compounds, whitening ingredients known in the art, substances that inhibit tyrosinase enzyme activity such as kojic acid and arbutin, hydroquinone, vitamin-C (L- Ascorbic acid) and its derivatives and various plant extracts may additionally contain one or two or more ingredients selected from the group consisting of. Additional ingredients may be included in an amount of 0.0001% to 10% by weight based on the total weight of the composition, and the content range may be adjusted according to requirements such as skin safety and ease of formulation of the pea extract.

Additionally, the composition of the present invention may further include a pharmaceutically acceptable carrier.

Pharmaceutically acceptable carriers may contain various ingredients such as buffers, sterile water for injection, normal saline or phosphate buffered saline, sucrose, histidine, salts and polysorbates.

The composition of the present invention can be administered orally or parenterally, and can be administered in the form of a general pharmaceutical preparation, for example, various oral and parenteral formulations during clinical administration. When formulated, commonly used fillers and extenders are used. It can be prepared using diluents or excipients such as binders, wetting agents, disintegrants, and surfactants.

Solid preparations for oral administration include tablets, pills, powders, granules, capsules, etc., and these solid preparations contain at least one excipient, such as starch, calcium carbonate, and water, in the pharmaceutical composition of the present invention. It can be prepared by mixing sucrose, lactose, gelatin, etc.

In addition to simple excipients, lubricants such as magnesium styrate talc are also used. Liquid preparations for oral use include suspensions, oral solutions, emulsions, syrups, etc. In addition to the commonly used simple diluents such as water and liquid paraffin, various excipients such as wetting agents, sweeteners, fragrances, and preservatives may be included.

Preparations for parenteral administration include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. Non-aqueous solvents and suspensions may include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, and injectable ester such as ethyl oleate. As a base for suppositories, witepsol, macrogol, tween 61, cacao, laurin, glycerogeratin, etc. can be used.

In the present invention, 'skin regeneration effect' refers to the recovery of skin tissue from damage caused by external and internal causes. Damage due to external causes may include ultraviolet rays, external contaminants, wounds, trauma, etc., and damage due to internal causes may include stress.

In the present invention, 'effective amount' refers to the amount of extract that can promote regeneration of damaged skin. When the composition of the present invention contains an effective amount of the above-mentioned pea extract, it can provide a desirable skin regenerative effect. The effective amount of the pea extract included in the composition of the present invention will vary depending on the form in which the composition is commercialized, the method by which the compound is applied to the skin, and the time it remains on the skin. For example, when the composition is commercialized as a pharmaceutical formulation, it may contain the pea extract at a higher concentration than when it is commercialized as a cosmetic product that is routinely applied to the skin. Therefore, the daily dosage is 0.1 to 100 mg/kg, preferably 30 to 80 mg/kg, more preferably 50 to 60 mg/kg, 1 to 6 times a day, based on the amount of the pea extract. may be administered.

The composition of the present invention can be used alone or in combination with surgery, radiation therapy, hormone therapy, chemical therapy, and methods using biological response modifiers.

The pharmaceutical formulation of the present invention may include a skin topical formulation.

When the pea extract is used as an external skin agent, fatty substances, organic solvents, solubilizers, thickeners and gelling agents, softeners, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, surfactants, and water are added. , ionic or non-ionic emulsifiers, fillers, sequestering agents and chelating agents, preservatives, vitamins, blocking agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles, or commonly used in external preparations for the skin. It may contain adjuvants commonly used in the field of dermatology, such as any other ingredients. Additionally, the ingredients may be introduced in amounts commonly used in the field of dermatology.

When the black bean extract is provided in a skin external formulation, it is not limited thereto, but may have formulations such as ointment, patch, gel, cream, or spray.

Additionally, the composition for skin regeneration of the present invention can be used to prepare cosmetic formulations.

The cosmetic formulation may be in the form of a general emulsified formulation or solubilized formulation. For example, lotion such as flexible lotion or nourishing lotion, emulsion such as facial lotion, body lotion, cream, essence, cosmetic ointment, spray, gel, pack, sunscreen, makeup base, liquid such as nutritional cream, moisture cream, eye cream, etc. It may have a formulation such as foundation type, solid type or spray type, powder, makeup remover such as cleansing cream, cleansing lotion, cleansing oil, cleansing foam, soap, body wash, etc.

In addition to the pea extract, the cosmetics contain fatty substances, organic solvents, solubilizers, thickeners and gelling agents, softeners, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, surfactants, water, and ionic agents. or non-ionic emulsifiers, fillers, sequestering and chelating agents, preservatives, vitamins, blocking agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or any other commonly used in cosmetics. It may contain auxiliaries commonly used in the field of cosmetics, such as ingredients.

The cosmetic formulation may contain a relatively high concentration of the pea extract in the case of wash-off type cosmetics such as makeup removers and detergents in which the active ingredient stays on the skin for a short period of time. On the other hand, in the case of leave-on type cosmetics such as lotions, emulsions, creams, and essences, in which the active ingredients stay on the skin for a long period of time, even if they contain a lower concentration of the pea extract than wash-off type cosmetics, It will be fine. Although not limited thereto, in one embodiment of the present invention, the composition may include the pea extract in an amount of 0.0001% to 10% by weight (preferably 0.0001% to 1% by weight) based on the total weight of the composition. . If the composition of the present invention contains less than 0.0001% by weight of the pea extract, sufficient skin regenerative effect cannot be expected, and if it contains more than 10% by weight, unwanted reactions such as allergies may occur or problems with skin safety may occur. This is to prevent this from happening.

Additionally, the composition for skin regeneration of the present invention can be used to prepare food formulations.

The food formulation refers to a food manufactured by adding the pea extract to food materials such as beverages, teas, spices, gum, and confectionery, or by encapsulating, powdering, or suspending it.

Since the food formulation can be consumed on a daily basis, it is very useful as it can be expected to have a high skin regeneration effect.

When using the pea extract as a food additive, the pea extract can be added as is or used together with other foods or food ingredients, and can be used appropriately according to conventional methods. The mixing amount of the active ingredient can be appropriately determined depending on the purpose of use (prevention, health, or therapeutic treatment). Generally, when producing a food or beverage, the composition of the present invention is added in an amount of 15 parts by weight or less, preferably 10 parts by weight or less, based on the raw materials. However, in the case of long-term intake for the purpose of health and hygiene or health control, the amount may be below the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount above the above range. .

There are no special restrictions on the types of foods above. Examples of foods to which the above substances can be added include meat, sausages, bread, chocolate, candies, snacks, confectionery, pizza, ramen, other noodles, gum, dairy products including ice cream, various soups, beverages, tea, drinks, etc. These include alcoholic beverages and vitamin complexes, and include all health foods in the conventional sense.

When the food formulation is a beverage, it may contain various flavoring agents or natural carbohydrates as additional ingredients, like regular beverages. The above-mentioned natural carbohydrates include monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As a sweetener, natural sweeteners such as thaumatin and stevia extract or synthetic sweeteners such as saccharin and aspartame can be used. The proportion of the natural carbohydrate is generally about 0.01 to 0.04 g, preferably about 0.02 to 0.03 g, per 100 mL of the composition of the present invention.

In addition to the above, food formulations include various nutrients, vitamins, electrolytes, flavors, colorants, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, carbonated drinks. It may contain carbonating agents used in. Additionally, the food formulation may contain pulp for the production of natural fruit juices, fruit juice drinks and vegetable drinks. These ingredients can be used independently or in combination. The ratio of these additives is not very important, but is generally selected in the range of 0.01 to 0.1 parts by weight per 100 parts by weight of the composition of the present invention.

The present invention also provides a composition for promoting stem cell activity containing the above-mentioned pea extract as an active ingredient.

In the present invention, the term ‘stem cell’ is a cell that is capable of cell division on its own and has the ability to differentiate into a wide variety of specific cell types. The type of these stem cells is not particularly limited, and in one embodiment, the stem cells may be skin stem cells. The ‘skin stem cells’ refer to stem cells that can differentiate into cells forming the skin (epidermis, dermis, and subcutaneous fat layer). The cells that make up the skin include keratinocytes and melanocytes present in the epidermis, and fibroblasts (mainly responsible for the biosynthesis of collagen and elastin) present in the dermis.

The type of skin stem cells is not particularly limited. The skin stem cells used in the present invention can be used regardless of where they originate. For example, skin stem cells can be obtained from a known skin stem cell source, for example, hair follicles or the basal layer of the epidermis, and the animal to be collected may be a mammal. In one embodiment, the mammal may include, but is not limited to, a human, mouse, rat, guinea pig, rabbit, monkey, pig, horse, cow, sheep, antelope, dog, or cat. Preferably the mammal may be a human. Methods for obtaining skin stem cells from skin stem cell sources are well known in the art.

In the present invention, the term 'stem cell activity promotion effect' refers to a stem cell proliferation promotion effect and/or a stem cell property maintenance effect.

Additionally, the composition of the present invention can be used for preparing cosmetic formulations. The contents of this cosmetic formulation are the same as those previously described in the description of the cosmetic formulation containing pea extract.

Hereinafter, the present invention will be described in detail by examples. However, the following examples only illustrate the present invention, and the content of the present invention is not limited to the following examples.

Manufacturing example 1: Preparation of pea extract

The soybeans were washed, powdered, and cold-soaked in 10 times ethanol (w/v) for 5 days, and then the extracted crude solution was filtered to obtain a concentrated extract. A mixed solution of purified water and ethanol was added thereto, dissolved, and then filtered to prepare the final extract.

Manufacturing example 2: Serum-free Under badge conditions skin stem cells culture

Human epidermal stem cells purchased from Cellntec were placed in a 96-well plate (5×10 ³ cells/well) and BPE (Bovine pituitary extract) similar to fetal bovine serum was added. CNT-57 medium (Cellntec) was used and cultured under conditions of 5% CO ₂ and 37°C for 24 hours. Afterwards, the culture medium was removed through the suction tube, the medium components were removed using a PBS solution (GibcoBRL), and the CNT-57 medium without BPE was treated with 5㎍/ml of the pea extract and incubated with 5% CO ₂ and Cultured for 72 hours at 37°C.

Experiment example One: CCK -8 Through evaluation method skin stem cells Proliferation promoting effect

CCK-8 (Cell counting kit-8) evaluation was performed on the skin stem cells cultured in Preparation Example 2. CCK-8 evaluation is an analysis method that indirectly indicates the density of living cells by measuring the absorbance of formazan, which is produced by dehydrogenase in the intracellular electron transport system by decomposing tetrazolium salt.

The cells were treated with CCK-8 solution (1/10 of the medium volume) at 37°C for 2 hours, and then the absorbance was measured spectroscopically at a wavelength of 450 nm. The effect of the pea extract on promoting the proliferation of skin stem cells was determined by calculating the proliferation rate (%) compared to the control group (CNT-57 medium without extract) from the measured absorbance value according to Equation 1 below, and the values are shown in Table 1 below. .

[Equation 1]

Proliferation rate (%) = (absorbance of sample treatment group/absorbance of control group) × 100

Effect of promoting proliferation of skin stem cells (number of repetitions = 3) Added sample density Cell proliferation rate (%) compared to control group Pea Extract 5㎍/ml 132% control group - 100%

As shown in Table 1, skin stem cells in medium conditions treated with pea extract showed an excellent proliferation promotion effect.

Experiment example 2: skin stem cells Stem cell type ( stemness ) Check the maintenance effect

To confirm the effect of maintaining stemness of skin stem cells cultured in Preparation Example 2, the level of p63 expression was evaluated. p63 is an intracellular transcription factor and is well known as a maintenance marker for skin stem cells. Cell RNA was isolated, cDNA was synthesized, and real-time PCR was performed using Taqman staining solution to measure the expression level of p63. In this experiment, the concentration of RNA was normalized to S16 ribosomal RNA.

The experimental results are shown in Table 2 below. In Table 2 below, the p63 fold increase value refers to the fold increase compared to the control group (CNT-57 medium with BPE added).

Effect of maintaining stemness (number of repetitions = 3) Added sample density p63 increase fold (fold) Pea Extract 5 ㎍/mL 2.54 control group - 1.0

As shown in Table 2, the medium conditions treated with pea extract promote the expression of p63 in skin stem cells and have an excellent effect of maintaining stem cell properties.

example 1: Preparation of pharmaceutical preparations

1. Preparation of tablets

Pea extract 0.2mg

Corn starch 100mg

Lactose 100mg

Magnesium stearate 2mg

After mixing the above ingredients, tablets were manufactured by tableting according to a conventional tablet manufacturing method.

example 2: Manufacturing of cosmetics

1. Manufacturing of nutritious cream

As shown in the composition below, a nutritional cream containing pea extract as an active ingredient was prepared according to a conventional method.

Pea extract 0.2% by weight

Beta-1,3-glucan 5.0% by weight

Beeswax 10.0% by weight

Polysorbate 60 1.5% by weight

PEG 60 hydrogenated castor oil 2.0% by weight

Sorbitan sesquioleate 0.5% by weight

Liquid paraffin 10.0% by weight

Squalane 5.0% by weight

Caprylic/capric triglyceride 5.0% by weight

Glycerin 5.0% by weight

Butylene glycol 3.0% by weight

Propylene glycol 3.0% by weight

Triethanolamine 0.2% by weight

Preservative 0.05% by weight

Pigment 0.05% by weight

Fragrance 0.05% by weight

Purified water to 100% by weight

example 3: Preparation of external skin preparations

1. Preparation of ointment

As shown in the composition below, an ointment containing pea extract as an active ingredient was prepared according to a conventional method.

Pea extract 0.5% by weight

Beta-1,3-glucan 10.0% by weight

Beeswax 10.0% by weight

Polysorbate 60 5.0% by weight

PEG 60 hydrogenated castor oil 2.0% by weight

Sorbitan sesquioleate 0.5% by weight

Vaseline 5.0% by weight

Liquid paraffin 10.0% by weight

Squalane 5.0% by weight

Shea butter 3.0% by weight

Caprylic/capric triglyceride 5.0% by weight

Glycerin 10.0% by weight

Propylene glycol 10.2% by weight

Triethanolamine 0.2% by weight

Preservative 0.05% by weight

Pigment 0.05% by weight

Fragrance 0.05% by weight

Purified water to 100% by weight

Claims (7)

A cosmetic composition for skin regeneration containing pea extract as an active ingredient.
According to paragraph 1,
The soybean extract is obtained by extracting the soybeans with one or more selected from the group consisting of water and an organic solvent, wherein the organic solvent includes a polar solvent including a lower alcohol having 1 to 5 carbon atoms, ethyl acetate, or acetone; Nonpolar solvents including ether, chloroform, benzene, hexane, or dichlorohexane; Or a cosmetic composition for skin regeneration, which is a mixed solvent thereof.
A food composition for skin regeneration containing pea extract as an active ingredient.
According to paragraph 3,
The soybean extract is obtained by extracting the soybeans with one or more selected from the group consisting of water and an organic solvent, wherein the organic solvent includes a polar solvent including a lower alcohol having 1 to 5 carbon atoms, ethyl acetate, or acetone; Nonpolar solvents including ether, chloroform, benzene, hexane, or dichlorohexane; Or a food composition for skin regeneration, which is a mixed solvent thereof.
A pharmaceutical composition for healing skin wounds containing pea extract as an active ingredient. delete delete