KR101668357B1 - Composition for improving skin conditions and method for improving skin conditions using the same - Google Patents

Abstract

The present invention relates to a composition for improving skin condition containing a plant extract as an active ingredient and a method for improving skin condition using the same. More specifically, the present invention relates to a composition for improving skin condition comprising plant extracts of Timus Linnaeus, a collagen root extract, , Winter Begonia root extract, Timus rinarelis leaf extract, Korakapuri extract and Princepsia utiliatus seed extract as an active ingredient, and a skin condition improving method using the composition. The composition according to the present invention is excellent in skin regeneration, skin elasticity and skin whitening efficacy, and has excellent efficacy for preventing, ameliorating or treating skin wrinkles, skin aging, skin inflammation, skin pigmentation.

Description

TECHNICAL FIELD The present invention relates to a composition for improving skin condition and a method for improving skin condition using the composition.

The present invention relates to a composition for improving skin condition containing a plant extract as an active ingredient and a method for improving the skin condition using the same. More specifically, the present invention relates to a composition for improving skin condition comprising plant extracts and a collagen root extract, The present invention relates to a composition for improving skin condition containing an extract, Timus linalis leaf extract, Korakapuri extract and Princepsia utiliatus seed extract as an active ingredient, and a skin condition improving method using the same.

Collagen, a major component of the extracellular matrix, is a major substrate protein produced by the fibroblasts of the skin. In addition, it is an important protein occupying about 30% of the total weight of the bioprotein, and has a solid triple helix structure. Collagen forms most of the organic matter in the skin, tendons, bones and teeth, especially in bone and skin (dermis). In most other body structures, it exists as a fibrous inclusion.

Collagen is a relatively weak immunogen, partly due to the blocking of the potential antigenic determinant by the helical structure of the collagen, which also causes the collagen to be resistant to proteolysis. The main functions of collagen are known to be mechanical rigidity of skin, resistance of connective tissues and binding force of tissues, support of cell adhesion, induction of cell division and differentiation (at the time of organ growth or wound healing) (Non-Patent Document 1) .

Such collagen is reduced by aging and photoaging due to ultraviolet irradiation, which is known to be closely related to the formation of wrinkles of the skin (Non-Patent Document 2). Collagen also plays an important role in wound healing and can accelerate the synthesis of collagen in the damaged epithelium, allowing quick, scarless wound healing.

Conventionally, products using collagen in a composition for external application for skin such as cosmetics or ointment have been marketed in order to utilize the skin moisturizing effect and wound healing effect of collagen. However, these products apply collagen itself to the skin surface, It is impossible to expect a moisturizing action or a wound healing effect, so that it can not be said that it exhibits essential skin function improvement and wound healing function.

In order to solve this problem, there has been a growing interest in collagen synthesis promoting substances. Conventional collagen synthesis promoting substances include vitamin C, retinoic acid, carcinogenic growth factor (non-patent document 3) (Patent Document 1), betulinic acid (Patent Document 2), chlorella extract (Patent Documents 3 and 4), and the like.

However, these materials have limited use due to safety problems such as irritation and reddening when applied to the skin, or the effect is insignificant and virtually no effect of skin function improvement or wound healing can be expected. Therefore, there is a desperate need to develop a novel composition for external skin application that is safer and more effective in living body than existing skin external composition compositions.

Melanin, which determines human skin color, is produced in melanocytes. Specifically, melanin is a black-brown pigment formed by a polymerization oxidation reaction with an enzyme such as tyrosinase existing in melanocytes, using tyrosine as an amino acid present in vivo as a substrate. The melanin thus formed is transferred to the epidermal cell called keratinocyte through the dendrites of melanocytes. Melanin, which has been transferred to keratinocytes, can be removed by keratinocytes falling off the skin when they fall off the epidermis. However, since there is no enzyme that degrades melanin in vivo, the once formed melanin is not degraded in vivo. Therefore, in order to lighten the skin, it is important to suppress the production of melanin.

Recently, the study of whitening agents as cosmetics has become increasingly necessary as skin blackness is recognized as skin aging due to ultraviolet rays, along with the improvement of living standards in Asian countries, which prefer emotionally white skin. Accordingly, substances exhibiting tyrosinase inhibitory activity such as ascorbic acid, hydroquinone, glutathione and arbutin have been used in cosmetics and pharmaceuticals (Patent Documents 5 and 6) 6). Most of them are ineffective or unstable because of their shape. In particular, a compound such as hydroquinone exhibits a strong decolorizing action, has a skin sensitization itself, can induce skin allergies and the like, and exhibits adverse effects such as inducing vitiligo by changing normal skin functions. Thus, Its use is limited.

In addition, even if the substance inhibits the activity of tyrosinase as described above, if such a substance acts on keratinocytes, which are keratinocytes, to increase the production of factors promoting melanin biosynthesis, the whitening action is consequently weakened The active inhibitor of tyrosinase is not considered to be a good whitening agent because it can cause harmful effects, and many tyrosinase inhibitors have been developed, but most of them are known to show no excellent whitening effect.

On the other hand, active oxygen introduced from the outside of the living body or generated in the living body causes many problems such as promoting aging of the living body, cancer, and the like. Therefore, the development and research of antioxidants that inhibit oxidation by active oxygen have been performed. Conventionally, there have been many attempts to obtain an anti-aging effect of skin by adding an antioxidant such as superoxide dismutase (SOD), vitamin E derivative, ascorbic acid and derivatives thereof, and flavonoid to cosmetics. However, The use thereof is limited due to problems in terms of stability in formulation.

Inflammation is the immune response of the body in response to a wound or disease. Oxidative stresses such as ultraviolet rays, active oxygen, and free radicals activate inflammatory factors and cause aging of various diseases and skin. The vasoactive polypeptide, kinin, plasmin and complement, have vasodilatory and contraction and chemotaxis effects, as well as lymphokines such as interleukin-6 (IL-6) Phosphorus and arachidonic acid are responsible for inflammation. Arachidonic acid is metabolized through inflammatory mediators such as prostaglandin and lukotrienes via two pathways: cyclooxygenase or lipooxygenase, mediating a variety of inflammatory responses.

On the other hand, in order to eliminate inflammation, elimination of inflammation source, reduction of vital reaction and symptoms is called anti-inflammatory. To date, there have been a number of nonsteroidal anti-inflammatory agents, including flufenamic acid, ibuprofen, benzydamine, indomethacin, prednisolone, , Dexamethasone, and the like. It is also known that allantoin, azene, hydrocortisone and the like are effective for antiinflammation. However, these materials are limited in their use due to safety of skin, .

Japanese Patent Application Laid-Open No. 8-231370 Japanese Patent Application Laid-Open No. 8-208424 Japanese Patent Application Laid-Open No. 9-40526 Japanese Patent Application Laid-Open No. 10-36283 Korean Patent Registration No. 10-0923141 Korean Patent Registration No. 10-0921947

Van der Rest et al., Ann NY Acad Sci, 1990 Arthur K. Balin et al., Aging and the skin, 1989 Cardinale G. 35 al, Adv. Enzymol., 41, p. 425, 1974

The present invention aims to provide a composition for improving skin condition containing Timus rinarelis leaf extract, collagen root extract or a mixture thereof as an active ingredient and a method for improving skin condition using the same.

Specifically, one object of the present invention is to provide a method for improving skin elasticity, preventing wrinkles or improving skin, improving skin irritation, containing the extract of Timus Linneais leaf, Korakapuri extract or a mixture thereof as an active ingredient A cosmetic composition for improving skin whitening, a quasi-drug composition or a food composition.

Another object of the present invention is to provide a pharmaceutical composition for preventing or treating a skin disease, which comprises a leaf extract of Timus Linneais, a collagen root extract or a mixture thereof as an active ingredient.

Another object of the present invention is to provide a method for regenerating skin containing the mixture of Winter Begonia root extract, Timus rinarelis leaf extract, Korakapuri extract and Princepsia utiliatus seed extract as an active ingredient, for improving skin elasticity, A cosmetic composition for improving skin irritation or for improving skin whitening, a quasi-drug composition or a food composition.

Another object of the present invention is to provide a pharmaceutical composition for prevention or treatment of skin diseases containing as an active ingredient a mixture of Winter Begonia root extract, Timus rinarelis leaf extract, Korakapuri extract and Princepsia utiliatus seed extract will be.

It is still another object of the present invention to provide a method for improving the skin condition or preventing or treating skin diseases using the above compositions.

As one embodiment for solving the above-mentioned problems, the present invention provides a composition for improving the skin condition containing Timus linalis leaf extract, collagen root extract or a mixture thereof as an active ingredient.

The present invention also provides a composition for improving skin condition containing as an active ingredient a mixture of Winter Begonia root extract, Timus rinarelis leaf extract, Korakapuri extract and Princepsia uttilis seed extract.

The present inventors have conducted studies on substances that are not toxic to naturally occurring natural resources and have excellent skin condition-improving effects, and the inventors of the present invention have found that the extract of Timus rinarelis leaf, collagen root extract or a mixture thereof is effective in promoting collagen synthesis, Suppression of melanin synthesis and inhibition of NO production, and have completed the composition for improving skin using the same. In addition, the extract of Winter Begonia root extract, Timus linalis leaf extract, Korakapuri extract and Princepsia utiliatus seed extract promotes collagen synthesis, inhibits collagenase activity, inhibits melanin synthesis, inhibits free radical scavenging and NO production And to complete a composition for improving skin using the same.

In the present invention, Angelica Glauca is a mountain type plant which grows in the northwest of the Himalayas at 1500 to 3700 meters above sea level. It is used as a medicinal plant. It is used as a beverage, a stimulant, a blowing agent, an expectorant, a sweating agent, and has been used for digestive disorders, gastrointestinal disorders, menstrual overgrowth and constipation. Roots have been used as spices and the like. Examples of the component include coumarin such as isoimperatorin, prangolarin and tert-C-methyloxypentadine hydrate, lactones such as angelicolide, (lactone), essential oil components (β-phellandrene) and (Z) -ligustilide ((Z) -ligustilide) have been reported.

Winter Begonia ( Bergenia Ciliata ) is a succulent plant that was used as a remedy in traditional Ayurvedic medicine. Nepal has also been used as a remedy for diabetes. Studies on various physiological activities have also been carried out and have been reported to be active against antibacterial, anti-inflammatory, anti-diabetic, and vincristine effects. Examples of the components include bergenin, catechin, gallic acid, gallicin, catechin-7-0-glucoside,? -Sitosterol (? sitosterol) have been reported.

Thymus linearis is a plant of Lamiaceae and grows in rock cliffs 2000 to 4000 meters above sea level in the Himalayan Karakoram area. Flowers and leaves have been used as herbal tea and spices, and have been used for vision loss, gastrointestinal disorders, urination and menstrual irregularities. It has been reported for essential components such as thymol, p-cymene, and γ-terpinene.

Prinsepia Utilis is a Rosaceae plant that is distributed in the Himalayas. Seed oil is used as edible oil or lamp oil for medicinal purposes. It is used for external use in rheumatism or muscular pain. Of the patients. In addition, the oil was used for the stomach of the abdomen, or for the poultice on the white line or the eczema area.

The term "extract" used in the present invention refers to an extract obtained by extracting the plant, a diluted solution or concentrate of the extract, a dried product obtained by drying the extract, a preparation or purified product of the extract, And mixtures thereof, extracts of all the formulations which can be formed using the extract itself and the extract.

In the plant extract of the present invention, the method for extracting the plant is not particularly limited, and may be extracted according to a method commonly used in the art. Non-limiting examples of the extraction method include hydrothermal extraction, ultrasonic extraction, filtration or reflux extraction, and they may be performed alone or in combination of two or more methods.

The kind of the extraction solvent used for extracting the plant in the present invention is not particularly limited, and any solvent known in the art can be used. Non-limiting examples of the extraction solvent include water; C ₁ to C ₄ lower alcohols such as methanol, ethanol, propyl alcohol and butyl alcohol; Polyhydric alcohols such as glycerin, butylene glycol and propylene glycol; And hydrocarbon solvents such as methyl acetate, ethyl acetate, acetone, benzene, hexane, diethyl ether, and dichloromethane; Or a mixture thereof. Water, a lower alcohol, 1,3-butyleneglycol, and ethyl acetate may be used alone or in admixture of two or more. In the present invention, water (or distilled water) or a lower alcohol may be used as a solvent for extracting the plant. The plant extract may be prepared by extracting each plant more than once using the above-mentioned solvent, and the dry extract obtained by vacuum distillation or spray drying the solvent extract may be prepared. In one specific embodiment of the present invention, plant extracts were prepared using water (Example 1).

In the present invention, the extract obtained by heat extraction or cold extraction is filtered to remove suspended solid particles by filtration, for example, nylon or the like, or filtered using a freezing filtration method or the like, Spray drying and the like.

The plant extract of the present invention comprises a fraction of an extract, and the fraction refers to a result obtained by performing fractionation to separate a specific component or a specific component group from a mixture containing various components.

The fractionation method for obtaining the fraction in the present invention is not particularly limited and may be carried out according to a method commonly used in the art. As a non-limiting example of the above-mentioned fractionation method, there can be mentioned a method of obtaining a fraction from the above extract by treating the extract obtained by extracting the plant with a predetermined solvent.

The kind of the fraction solvent used for obtaining the fraction in the present invention is not particularly limited, and any solvent known in the art can be used. Non-limiting examples of the fraction solvent include polar solvents such as water, distilled water and alcohol; And non-polar solvents such as hexane, ethyl acetate, chloroform, and dichloromethane. These may be used alone or in combination of two or more. When alcohols are used in the fraction solvent, C ₁ to C ₄ alcohols can be preferably used.

The composition of the present invention is a mixture of the leaf extract of the present invention, the extract of Coracao root, the mixture of these or a mixture of these, or a mixture of Winter Begonia root extract, Timus linereis leaf extract, Korakapuri extract and Principia utiliatus seed extract Extract of the present invention has an effect of effectively increasing the total amount of collagen, specifically showing an effect of inhibiting collagenase activity and inhibiting the production of collagenase MMP-1 as an enzyme involved in increasing the total amount of collagen .

 According to a specific embodiment of the present invention, when treated with human fibroblast cellulitis leaf extract, collagen root extract or a mixture thereof, or a mixture of four species of Nepal plant extract, human fibroblasts It was confirmed that the total amount of collagen produced was increased (Example 2).

According to another embodiment, there is provided a method for producing MMP-1 which is a collagenase produced by treating a human fibroblast cellulase extract, a collagen extract or a mixture thereof, or a mixture of four plant extracts of Nepal plant, (Tumor necrosis factor-a), which is a human fibroblast, was inhibited (Example 3).

In addition, the mixture of the extract of Timus rinelis Leaf, the extract of Coracarpa, the mixture thereof or the mixture of four Nepal plant extracts of the present invention effectively inhibits the production of melanin. Specifically, the efficacy of reducing the total amount of synthesized melanin .

Melanin is a dark brown pigment produced in melanocytes, which determines the skin color of a person and can be expected to brighten the skin by inhibiting melanin pigmentation.

According to a specific embodiment of the present invention, a B-16 mouse melanoma cell is administered with a mixture of Timus Linnaeus leaf extract, Korakapuri extract or a mixture thereof, or a mixture of four species of Nepal plant extract It was confirmed that the melanin content of melanoma cells was reduced (Example 4).

The Nepal plant four extract mixture of the present invention exhibits excellent antioxidative efficacy. Specifically, the Nepal plant four-plant extract mixture of the present invention shows an effect of eliminating free radicals, and particularly, it has excellent effect of eliminating DPPH.

Free radicals are introduced from the outside of the living body or occur in the living body, which accelerates aging of the living body, causes cancer, and causes many problems.

According to a specific embodiment of the present invention, when the DPPH was added to the mixture of four plant extracts of Nepal, excellent free radical scavenging rates were observed (Example 5).

In addition, the mixture of the extract of the present invention, Timothy Lineris leaf extract, Coracarpa extract, or a mixture thereof or the Nepal plant four extracts exhibits excellent anti-inflammatory activity. Specifically, the mixture of the extract of T. reneiis leaf extract, collagen extract or mixture thereof or the mixture of four plant extracts of Nepal plant of the present invention exhibits an effect of inhibiting the production of NO, and in particular, as in the case of L-NMMA, The effect of inhibiting the production is excellent.

NO is produced by the NOS (NO synthase) enzyme, which can be activated by immunostimulation such as IFNγ, TNFα, and LPS.

According to a specific embodiment of the present invention, RAW264.7 cells, which are mouse macrophages, are treated with a mixture of Timus Linnea Leaf Extract, Korakapuri Extract or a mixture thereof or a Nepalese Plant Extract It was confirmed that when LPS (Lipopolysaccharide) was treated as an immunostimulant, the amount of NO produced was reduced (Example 6).

The term "improving skin condition" as used in the present invention means skin regeneration, skin elasticity improvement, skin wrinkle prevention or improvement, skin inflammation improvement or skin whitening improvement.

As used herein, the term "skin regeneration" or "skin elasticity improvement" or "skin wrinkle prevention or improvement" means all actions that inhibit collagenase activity and increase the total amount of collagen.

As used herein, the term "skin aging" refers to any disease caused by increased active oxygen, and examples of the skin aging include wrinkles, skin wrinkles, and reduced elasticity.

As used herein, the term "improvement of skin inflammation" means all actions in which the immune response is alleviated and NO production is suppressed.

As used herein, the term "skin whitening" means any action that inhibits the synthesis of melanin and inhibits or prevents skin deposition of melanin.

As used herein, the term "skin pigmentation" refers to any action or condition that causes skin color to darken due to increased synthesis of melanin.

As used herein, the term "improvement" means any action that at least reduces the degree of symptomatology, such as a parameter associated with relief or treatment of a condition.

According to one embodiment of the present invention, there is provided a method for regenerating skin, improving skin elasticity, preventing wrinkle or improving skin, improving skin inflammation, containing Timus Linnea Leaf Extract, Korakapuri Extract or a mixture thereof as an active ingredient Or a cosmetic composition for improving skin whitening.

Also provided is a cosmetic composition for skin regeneration, skin elasticity improvement, skin wrinkle prevention or improvement, skin aging prevention or improvement, skin irritation improvement or skin whitening improvement containing an Nepalese plant extract as an active ingredient .

The cosmetic composition of the present invention can be used as a cosmetic composition in the form of a solution, an external ointment, a cream, a foam, a nutritional lotion, a flexible lotion, a pack, a flexible water, an emulsion, a makeup base, But are not limited to, formulations selected from the group consisting of pastes, gels, lotions, powders, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations, patches and sprays.

The cosmetic composition of the present invention may further comprise at least one cosmetically acceptable carrier to be incorporated in a cosmetic composition for general skin. Examples of the cosmetic composition include ordinary ingredients such as oil, water, a surfactant, a moisturizer, a lower alcohol, , A chelating agent, a coloring agent, a preservative, a perfume, and the like may be appropriately compounded, but the present invention is not limited thereto.

The cosmetically acceptable carrier contained in the cosmetic composition of the present invention varies depending on the formulation of the cosmetic composition.

When the formulations of the present invention are ointments, pastes, creams or gels, the carrier component may be an animal oil, a vegetable oil, a wax, a paraffin, a starch, a tracer, a cellulose derivative, polyethylene glycol, silicon, bentonite, silica, talc, zinc oxide May be used, but is not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder and the like may be used as a carrier component. In particular, But are not limited to, propellants such as rocaborn, propane / butane or dimethyl ether. These may be used alone or in combination of two or more.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent may be used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, Propylene glycol, 1,3-butyl glycol oil and the like can be used, and particularly fatty acid esters of cottonseed oil, peanut oil, corn oil, olive oil, castor oil and sesame oil, glycerol aliphatic ester, polyethylene glycol or sorbitan May be used, but is not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspension such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Crystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, but are not limited thereto. These may be used alone or in combination of two or more.

According to another embodiment of the present invention, there is provided a method for regenerating skin, comprising improving the skin elasticity, preventing or wrinkling the skin, improving skin irritation, containing the extract of Timus Linneais leaf, Korakapuri extract or a mixture thereof as an active ingredient Or a quasi-product composition for skin whitening improvement.

Also provided is a quasi-product composition for skin regeneration, skin elasticity improvement, skin wrinkle prevention or improvement, skin aging prevention or improvement, skin inflammation improvement or skin whitening improvement composition containing a Nepalese plant extract mixture as an active ingredient .

The term "quasi-drug product" used in the present invention means products that are less active than drugs, among the products used for diagnosing, treating, improving, alleviating, treating or preventing diseases of human or animal. For example, Quasi-drugs are products that are used for the purpose of treating or preventing diseases of humans or animals, products that are mild or have no direct action on the human body.

The quasi-drug composition of the present invention can be prepared by a formulation selected from the group consisting of body cleansers, foams, masks, ointments, creams, lotions, essences and sprays, but is not limited thereto.

In the quasi-drug composition of the present invention, a description is given of the extract of Timus Linnea Leaf, the extract of Coracarpa or a mixture thereof, the description of the skin regeneration, skin elasticity improvement, skin wrinkle prevention or improvement, skin inflammation improvement and skin whitening improvement Are the same as those described above in connection with the cosmetic composition.

Further, in the quasi-drug composition of the present invention, explanation about the Nepal plant four-part extract mixture, description of skin regeneration, skin elasticity improvement, skin wrinkle prevention or improvement, skin aging prevention or improvement, skin inflammation improvement and skin whitening improvement, Are the same as those described above in connection with the cosmetic composition.

According to another embodiment of the present invention, there is provided a method for regenerating skin, comprising improving the skin elasticity, preventing or wrinkling the skin, improving skin irritation, containing the extract of Timus Linneais leaf, Korakapuri extract or a mixture thereof as an active ingredient Or for improving skin whitening.

The present invention also provides a food composition containing the Nepalese plant extract as an active ingredient for skin regeneration, skin elasticity improvement, skin wrinkle prevention or improvement, skin aging prevention or improvement, skin inflammation improvement or skin whitening improvement.

The food composition of the present invention is not particularly limited and includes a health functional food composition.

When the health functional food composition of the present invention is used as a food additive, the composition may be added as it is or may be used together with other food or food ingredients, and may be appropriately used according to a conventional method.

The kind of the food is not particularly limited, and includes food in a conventional sense. Non-limiting examples of the food to which the substance can be added include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gums, dairy products including ice creams, , A drink, an alcoholic beverage, and a vitamin complex.

When the health functional food composition of the present invention is a beverage composition, various flavoring agents, natural carbohydrates, and the like may be contained as additional components such as ordinary beverages. Non-limiting examples of such natural carbohydrates include monosaccharides such as glucose and fructose; Disaccharides such as maltose and sucrose; Natural sweetening agents such as dextrin, cyclodextrin; Synthetic sweetening agents such as saccharin and aspartame, and the like. The proportion of the additional component added may be appropriately determined by a person skilled in the art.

In addition to the above, the health functional food composition of the present invention may contain various nutrients, vitamins, electrolytes, flavors, colorants, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloid thickening agents, pH adjusting agents, stabilizers, , Alcohols, carbonating agents used in carbonated drinks, and the like. In addition, the health functional food composition of the present invention may contain pulp for the production of natural fruit juice, fruit drink or vegetable drink. These components may be used independently or in combination of two or more. The ratios of these additives can also be suitably selected by those skilled in the art.

In the food composition of the present invention, a description of the timus rinelis leaf extract, collagen root extract or a mixture thereof, description of skin regeneration, skin elasticity improvement, skin wrinkle prevention or improvement, skin inflammation improvement and skin whitening improvement Are the same as those described above in connection with the cosmetic composition.

The description of the Nepal plant four-part extract mixture, description of skin regeneration, skin elasticity improvement, skin wrinkle prevention or improvement, skin aging prevention or improvement, skin inflammation improvement and skin whitening improvement, .

In the cosmetic composition, quasi-drug composition or food composition of the present invention, Timus rinarelis leaf extract, collagen root extract or a mixture thereof may be contained in an amount of preferably 0.0001 to 50% by weight, And more preferably from 0.0005 to 10% by weight. Within the above range, there is an advantage that excellent skin regeneration, skin elasticity improvement, skin wrinkle prevention or improvement, skin inflammation improvement and skin whitening improvement effect are exhibited, and the formulation of the composition is stabilized.

Also, the Nepalese plant extract mixture may preferably be contained in an amount of 0.0001 to 50% by weight, more preferably 0.0005 to 10% by weight, based on the dry weight of the composition. Within the above range, there is an advantage that excellent skin regeneration, skin elasticity improvement, skin wrinkle prevention or improvement, skin aging prevention or improvement, skin inflammation improvement and skin whitening improvement effect, and the formulation of the composition is stabilized.

According to another embodiment of the present invention, there is provided a pharmaceutical composition for preventing or treating a skin disease, which comprises an extract of Timus Linneais leaf, a collagen root extract or a mixture thereof as an active ingredient.

The skin diseases include at least one selected from the group consisting of skin wounds, skin scars, skin inflammation, and skin pigmentation.

The present invention also provides a pharmaceutical composition for preventing or treating skin diseases, which comprises a Nepal plant four-extract mixture as an active ingredient.

The skin diseases include at least one selected from the group consisting of skin wounds, skin scars, skin aging, skin inflammation, and skin pigmentation.

In the above pharmaceutical composition of the present invention, the description of the thymus linea leaf extract, the collagen root extract or the mixture thereof, the skin inflammation, and the description of skin pigmentation are the same as described above in connection with the cosmetic composition .

Further, in the pharmaceutical composition of the present invention, explanation on the Nepal plant four-part extract mixture, skin aging, skin inflammation, and skin pigmentation are the same as described above in connection with the cosmetic composition.

The skin scars and skin scars of the present invention mean all diseases that can be treated due to an increase in the total amount of collagen in skin tissue. Non-limiting examples of skin wounds, skin scars include abrasions, scars caused by abrasions, and the like.

The skin inflammatory disease of the present invention means all diseases caused by immune stimulation. Non-limiting examples of the skin inflammatory diseases include atopic dermatitis, eczema, seborrheic dermatitis, psoriasis, acne and the like.

The skin pigmentation disease of the present invention means all diseases caused by skin pigmentation due to an increase in the production of melanin. Non-limiting examples of the skin pigmentation diseases include stain, freckles, spots, daylight pigment, pigmentation after drug use, inflammation pigmentation, and pregnancy pigmentation.

In the pharmaceutical composition of the present invention, the effective amount of the extract of Timus Linnea Leaf, Korakapuri extract, or a mixture thereof or the mixture of four species of Nepal plant extract is not particularly limited, and is preferably 0.00001% by weight to the total weight of the pharmaceutical composition, 99.99% by weight, or may be included in the total content.

The pharmaceutical composition of the present invention may further comprise a pharmaceutically acceptable carrier in addition to containing, as an active ingredient, a mixture of a Timus Linnaeus leaf extract, a Korakapuri extract or a mixture thereof or a Nepal plant four- .

In the present invention, the expression "pharmaceutically acceptable " means that it can be routinely used in the pharmaceutical field, without disturbing the biological activity and properties of the compound to be administered, without irritating the organism upon administration thereof.

The pharmaceutical composition of the present invention may be formulated together with the carrier to be used as food, medicines, feed additives, drinking water additives, and the like.

In the present invention, the type of the carrier is not particularly limited and any carrier conventionally used in the art can be used. Examples of the carrier include, but are not limited to, saline, sterilized water, Ringer's solution, buffered saline, albumin injection solution, lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, maltodextrin, glycerol, . These may be used alone or in combination of two or more.

In addition, the pharmaceutical composition of the present invention may be supplemented with other pharmaceutically acceptable additives such as excipients, diluents, antioxidants, buffers or bacteriostats, and may be used as fillers, extenders, wetting agents, disintegrants, , A binder, a lubricant, and the like may be additionally used.

The pharmaceutical composition of the present invention may be formulated into various formulations suitable for oral administration or parenteral administration.

Non-limiting examples of such oral dosage forms include troches, lozenges, tablets, aqueous suspensions, oily suspensions, prepared powders, granules, emulsions, hard capsules, soft capsules, syrups or elixirs .

In order to formulate the pharmaceutical composition of the present invention for oral administration, a binder such as lactose, saccharose, sorbitol, mannitol, starch, amylopectin, cellulose, or gelatin; Excipients such as dicalcium phosphate and the like; Disintegrating agents such as corn starch or sweet potato starch; Lubricants such as magnesium stearate, calcium stearate, sodium stearyl fumarate or polyethylene glycol wax may be used, and sweeteners, fragrances, syrups and the like may also be used. .

Furthermore, in the case of capsules, in addition to the above-mentioned substances, liquid carriers such as fatty oils can be further used.

Non-limiting examples of the parenteral preparation include injectable solutions, suppositories, respiratory inhalation powders, aerosol formulations for spray, mouth spray, mouthwash, toothpaste, ointment, powder for application, oil, have.

In order to formulate the pharmaceutical composition of the present invention for parenteral administration, sterilized aqueous solutions, non-aqueous solvents, suspensions, emulsions, freeze-dried preparations and external preparations may be used. Examples of the non-aqueous solutions and suspensions include propylene glycol, polyethylene Glycerol, vegetable oils such as olive oil, injectable esters such as ethyl oleate, and the like.

More specifically, when the pharmaceutical composition of the present invention is formulated into an injection, the composition of the present invention is mixed with water or a stabilizer or buffer in water to prepare a solution or suspension, which is then mixed with an ampoule or a vial Unit dosage form. When the pharmaceutical composition of the present invention is formulated into an aerosol formulation, a propellant or the like may be added together with the additive such that the water-dispersed concentrate or the wet powder is dispersed.

When the pharmaceutical composition of the present invention is formulated into an ointment, cream, or the like, it is possible to use an animal oil, a vegetable oil, a wax, a paraffin, a starch, a tracer, a cellulose derivative, a polyethylene glycol, a silicone, a bentonite, Or the like can be used as a carrier.

The pharmaceutical composition of the present invention may preferably be formulated into a parenteral dosage form, and more preferably, it may be a gel, a patch, a spray, an ointment, a warning agent, a lotion agent, a liniment agent, a pasta agent and a cataplasma agent Lt; RTI ID = 0.0 > a < / RTI >

The pharmaceutically effective amount and the effective dose of the pharmaceutical composition of the present invention may be varied depending on the formulation method, administration method, administration time and / or administration route of the pharmaceutical composition, and the reaction to be achieved by the administration of the pharmaceutical composition The type and severity of the subject to be treated, the type of subject to be treated, age, weight, general health condition, symptom or degree of disease, sex, diet, excretion, drugs used simultaneously or simultaneously with the subject, And other factors well known in the medical arts, and those skilled in the art will readily determine and prescribe dosages that are effective for the desired treatment. The pharmaceutical composition of the present invention may be administered once a day or divided into several doses. Accordingly, the dosage is not limited in any way to the scope of the present invention.

A preferred dosage of the pharmaceutical composition of the present invention may be from 1 mg / kg to 1, OOO mg / kg per day.

The route of administration and the mode of administration of the pharmaceutical composition of the present invention may be independent of each other, and the method is not particularly limited, and any route of administration and administration method may be used as long as the pharmaceutical composition can reach the desired site You can follow. The pharmaceutical composition may be administered orally or parenterally.

The parenteral administration may be, for example, intravenous administration, intraperitoneal administration, intramuscular administration, transdermal administration or subcutaneous administration, and a method of applying, spraying or inhalation the composition to a diseased site may also be used But are not limited to.

The pharmaceutical composition of the present invention may preferably be administered parenterally, more preferably, in a manner such that it is topically applied to a site where improvement of the skin condition, prevention, improvement or treatment of skin disease is required have.

As used herein, the term "prevention" refers to the administration of the pharmaceutical composition of the present invention to a subject to inhibit or delay the onset of skin diseases, including skin wounds, skin scars, skin inflammatory diseases or skin pigmentation diseases It means all acts.

As used herein, the term "treatment" refers to any action which, by administering the pharmaceutical composition of the present invention to a subject, may improve or improve the symptoms of skin wounds, skin scars, skin inflammatory diseases and skin pigmentation diseases it means.

According to another embodiment of the present invention, a cosmetic composition, a quasi-drug composition or a food composition containing an extract of Timus rinarelis leaf, a collagen root extract or a mixture thereof, or a mixture of four Nepalese plant extracts And a method for improving the skin condition.

The term "individual" as used herein refers to all animals, including mammals including rats, livestock, humans, and the like.

In the present invention, the cosmetic composition, the quasi-drug composition or the food composition containing the extract of Timus Linneais leaf, Korakapuri extract or a mixture thereof, or a mixture of four species of Nepal plant extract as an active ingredient is the same as described above, The meaning of improvement is also the same as described above.

In the present invention, the description of the dose, route of administration, mode of administration, and the like of the composition is the same as that described in connection with the pharmaceutical composition of the present invention.

According to another embodiment of the present invention, there is provided a method of treating skin, comprising administering to a subject a pharmaceutical composition comprising as an active ingredient, a mixture of a leaf extract of Timus linereis leaf, a collagen root extract or a mixture thereof, Thereby preventing or treating the disease.

In the present invention, the pharmaceutical composition containing the extract of Timus rinareis leaf, collagen root extract or a mixture thereof or a mixture of four species of Nepal plant extract as an active ingredient is the same as that described above, Is also the same as that described above.

In the present invention, the dosage, route of administration, mode of administration and the like of the pharmaceutical composition are the same as those described in connection with the pharmaceutical composition of the present invention.

The composition of the present invention containing a mixture of the extract of Timus rinarelis leaf, the extract of Coracarpa or a mixture thereof, or the mixture of four kinds of Nepal plant extracts is excellent in skin regeneration, skin elasticity, skin whitening effect, Skin aging, skin inflammation, and skin pigmentation. Specifically, the composition of the present invention is excellent in inhibiting the increase of the total amount of collagen, the decrease of the collagenase activity, the increase of the total amount of melanin, the free radical scavenging and the inflammatory reaction.

In addition, since the composition of the present invention is a substance derived from a natural material, it is not toxic and does not cause side effects even when it is applied to a human body, and thus its application to cosmetics, quasi-drugs, foods or pharmaceutical compositions is high.

Hereinafter, embodiments of the present invention will be described in detail to facilitate understanding of the present invention. However, the embodiments according to the present invention can be modified into various other forms, and the scope of the present invention should not be construed as being limited to the following embodiments. Embodiments of the invention are provided to more fully describe the present invention to those skilled in the art.

Preparation of extract

Winter Begonia root, Timus linalis leaf, Coracarpus and Prunus sepia utiliens were each well dried and finely divided, and 60 g of dry weight was added to each of the flasks and subjected to hot extraction with 1000 g of extraction solvent (water) for 4 hours . The hot water extract was filtered with a filter having a pore size of 0.2 탆 to prepare each plant extract. The mixture of Timus linalis leaf extract and Korakapuri extract mixture was prepared by mixing Timus rinelis leaf extract and Korakapuri extract in a ratio of 10: 1. Nepal plant extracts were prepared by mixing Winter Begonia root extract, Timus rinarelis leaf extract, Korakapuri extract and Princepsia utiliatus seed extract at a ratio of 10: 10: 1: 10. Each extract was concentrated under reduced pressure and then lyophilized or spray dried to obtain a dry extract.

Increase effect of total collagen

The extracts of the following Table 1 were added to the culture medium of human-derived fibroblasts to examine the effect of increasing the total amount of collagen at the cellular level.

sample One Timus lineris leaf extract 2 Korakapuri extract 1 + 2 1 + 2 mixture 1 + 2 + 3 + 4 Four Nepal plant extract mixture

The total amount of collagen was measured using a PICP EIA kit (Procollagen Type I C-Peptide Enzyme Immunoassay Kit). The human fibroblasts before the experiment were evaluated for their cytotoxicity by varying the concentration (ppm) of each of the plant extracts prepared in Example 1 and the mixture of the extracts at the above ratios (MTT test was performed by culturing fibroblasts The cytotoxicity-free concentration (100 ppm) was determined by the method of Mossman T. (1983) Rapid Colorimetric Assay for Cellular Growth & Survival: Application to proliferation and cytotoxicity assays. Journal of Immunological Methods 65, 55-63) Or less) to evaluate the degree of increase in the total amount of collagen.

MTT test

Each sample was added to the culture medium of human fibroblasts and cultured for 1 day. Then, the culture solution was taken and the degree of increase in the total amount of collagen at each concentration was measured at 450 nm using a PICP EIA kit using a spectrophotometer. For the comparison of the effects, the increase in the total amount of collagen was measured by the same method in the culture medium of fibroblasts (control group) to which nothing was added and the sample added with vitamin C to a final concentration of 52.8 ppm. The total amount of collagen was measured as the UV absorbance. The increase rate of the total amount of collagen was calculated as a ratio of the total amount of collagen relative to the control, and the effect of increasing the total amount of collagen (number of repeats = 3) is summarized in Table 2 below.

sample Absorbance average Collagen growth rate (%) ① Timus lineraris leaf extract (100ppm) 579 127 ② Korakapuri extract (100ppm) 565 124 ① + ② mixture (100ppm) 583 128 Four plant extracts of Nepal plant (100ppm) 617 135 Control group (no addition) 456 - Vitamin C (52.8ppm) 571 125

As can be seen from the results in Table 2, the extracts of Timus rinarelis leaf, collagen root extract, or a mixture thereof showed an effect of promoting collagen synthesis and increasing the total amount of collagen. In particular, there was an increase in the total amount of collagen.

Therefore, it was found that a mixture of the extract of Timus rinareis leaf, the extract of Korakapuri, a mixture thereof, or the Nepal plant four extracts can be used for skin regeneration and wrinkle improvement.

Collagenase  Activity inhibitory effect

The inhibitory effect on collagenase activity (number of repeats = 3) was confirmed as follows in the extracts of Table 3 below.

Human normal skin cells, fibroblasts, were inoculated into 24-well microplates at 2.5 × 10 ⁴ cells per well, cultured in 10% serum DMEM medium and 37 ° C. for 24 hours, and 10% serum DMEM medium was removed The cells were washed once with phosphate-buffered saline, and further incubated for 30 minutes in serum-free DMEM medium supplemented with each of the extracts shown in Table 3 below and in serum-free DMEM medium containing no extract.

After 30 minutes of sample treatment, the cells were cultured for 24 hours after stimulation with 50 ng / ml TNF-α (tumor necrosis factor-α), a substance known to produce collagenase MMP-1. The TNF-α-untreated group and the treated group were treated with TNF-α-untreated control group and TNF-α-treated control group, respectively.

The supernatant of each well was collected and the amount (ng / ml) of the newly synthesized MMP-1 was measured using an MMP-1 assay kit (Amersham, USA). The inhibition rate of collagenase activity was determined by MMP -1 production inhibition rate (%) was calculated. The results are shown in Table 3 below.

The amount of MMP-1 in the TNF-treated group represents the amount of MMP-1 in the negative control group and the amount of MMP-1 in the positive control group represents the amount of MMP-1 in the TNF treated group. Were added at concentrations of 100 ppm, respectively.

[Equation 1]

MMP-1 production inhibition rate (%) =

(X = amount of test group MMP-1, Y = amount of positive control MMP-1, Z = amount of negative control MMP-1)

sample The amount of MMP-1 (ng / ml) Inhibition rate (%) ① Timus lineraris leaf extract (100ppm) 10.75 60.11 ② Korakapuri extract (100ppm) 11.38 54.27 ① + ② mixture (100ppm) 10.12 65.96 Four plant extracts of Nepal plant (100ppm) 9.54 71.34 Negative control (TNF untreated) 6.45 - The positive control (TNF treatment) 17.23 -

As can be seen from the results in Table 3, it can be seen that the mixture of the extract of Timus rinareis leaf, Korakapuri extract, or a mixture thereof and the Nepal plant 4 extract has excellent inhibitory ability of collagenase MMP-1 . Considering that each is a simple extract of plant rather than a single ingredient, it has a very good collagen-increasing effect.

Therefore, it was found that a mixture of the extract of Timus rinareis leaf, the extract of Korakapuri, a mixture thereof, or the Nepal plant four extracts can be used for skin regeneration and wrinkle improvement.

Whitening effect - Confirmation of total melanin reduction effect

The extracts of the following Table 4 were added to the culture medium of rat melanoma cells (B-16 mouse melanoma cells) to examine the whitening effect at the cellular level.

Each of the extracts shown in Table 4 was diluted to a final concentration of 100 ppm and the control group, arbutin, was added to the medium to a concentration of 100 ppm, which was then treated with B-16 melanoma cells and cultured for 3 days.

Cells were then trypsinized, detached from the culture, centrifuged, and extracted with melanin. The removed cells were melted with 1 ml of sodium hydroxide solution (1N concentration), boiled for 10 minutes, melanin was dissolved, and the amount of melanin produced by measuring the absorbance at 400 nanometers (nm) was measured using a spectrophotometer.

The amount of melanin was measured by an absorbance of 10 ⁶ cells per unit cell, calculated as the inhibition rate (%) relative to the total amount of melanin relative to the control, and the result (the effect of decreasing total melanin) is summarized in Table 4 below. The experiment was repeated three times.

sample Total melanin (Abs) Inhibition rate (%) ① Timus lineraris leaf extract (100ppm) 0.065 20.73 ② Korakapuri extract (100ppm) 0.062 24.39 ① + ② mixture (100ppm) 0.064 21.95 Four plant extracts of Nepal plant (100ppm) 0.055 32.93 Control group (no addition) 0.082 - Arbutin (100 ppm) 0.060 26.83

As can be seen from the results of Table 4, the extract of Timus Linnea Leaf, Coracarpa extract or a mixture thereof is less effective than arbutin, which is a positive control, but is a simple extract and exhibits excellent melanin total reducing effect inhibiting melanin synthesis And it was confirmed that the mixture of four plant extracts of Nepal showed a better total melanin reduction effect than arbutin, which is a positive control, and thus it can be used for whitening purposes.

Antioxidative effect- Free radical  Erasure rate

Free radical scavenging activity was measured to examine the antioxidative activity of the four plant extracts of Nepal. Free radical scavenging activity was measured using DPPH. DPPH is a relatively stable free radical, which shows maximum absorption at 517 ㎚ in the presence of radicals and loses its absorbance when radicals are eliminated. DPPH was purchased from Sigma Co., Ltd, USA and dissolved in methyl alcohol at a concentration of 0.15 mM.

First, 100 μl of the Nepal plant four-extract mixture and ascorbic acid were added to each well of a 96-well plate. 100 μl of DPPH solution was added thereto, and the mixture was allowed to stand at room temperature for 30 minutes. Then, the absorbance at 517 nm was measured using a microplate reader (BioTek EL-340). At this time, methyl alcohol was added in place of the sample to give a control group. Free radical scavenging activity and the results are obtained for the IC ₅₀ half maximal inhibitory concentration (median inhibition) shown in Table 5. IC ₅₀ is a common method of expressing free radical scavenging ability as a concentration of ascorbic acid and a mixture of four plant extracts of Nepal plant, which is required to remove 50% of the free radicals of the no-added control group.

division IC ₅₀ Four Nepal plant extract mixture 0.0032% Ascorbic acid 0.0007%

As shown in Table 5 above, the Nepal plant four-extract mixture of the present invention has a lower free radical scavenging ability than that of ascorbic acid, considering that it is a simple extract of plants.

Anti-inflammatory effect - NO  Production inhibitory effect

In order to confirm the anti-inflammatory effect and the skin troubles-relieving effect of the mixture of the extracts of Tymoschinelis leaf, Korakapuri extract or mixture thereof and the four plant extracts of Nepal plant, RAW264.7 cell line (ATCC number: CRL-2278) The nitric oxide (NO) formation inhibition test was performed by GRIESS method.

Specifically, RAW264.7 cells, macrophages of mice, were subcultured several times, placed in 24-well plates at 3 × 10 ⁵ per well, and cultured for 24 hours. Then, the cell culture medium containing the extracts of Timus rinelis leaf extract, collagen root extract or a mixture thereof, and a mixture of four plant extracts of Nepal plant was diluted with the concentrations shown in Table 6 below. At this time, L-NMMA (L-NG-Monomethylarginine), a NO-production inhibitor, was treated together as a positive control and cultured for 30 minutes. Lipopolysaccharide (LPS) . 100 μl of the supernatant was transferred to a 96-well plate, and 100 μl of GRIESS solution was added thereto at room temperature for 10 minutes. The absorbance at 540 nm was measured to determine the NO inhibitory effect of each extract and extract mixture. Are shown in Table 6 below.

sample NO production inhibition rate (%) ① Timus lineraris leaf extract (10ppm) 31.34 ② Korakapuri extract (10ppm) 38.95 ① + ② mixture (10ppm) 35.82 Four plant extracts of Nepal plant (10ppm) 43.14 L-NMMA (10 ppm) 39.54

As shown in Table 6, the efficacy of the extract of the present invention of the present invention is lower than that of L-NMMA, which is a representative efficacious substance, Of the respondents.

In addition, it was confirmed that the mixture of four plant extracts of Nepal showed a superior NO production inhibitory effect than L-NMMA.

Formulation example  1: Preparation of pharmaceutical preparations

The following Table 7 shows the extract and the mixture of the extract of the present invention.

sample ① Timus lineraris leaf extract ② Korakapuri extract ① + ② mixture Four Nepal plant extract mixture

1. Manufacturing of powder

One of the extracts or extract mixtures of Table 7 above, 0.001 g

Lactose 1g

The above components were mixed and packed in airtight bags to prepare powders.

2. Preparation of tablets

One of the extracts or extract mixtures of Table 7 above, 0.2 mg

100 mg of corn starch

100 mg of milk

Stearic acid  Magnesium 2 mg

After mixing the above components, tablets were prepared by tableting according to a conventional method for producing tablets.

3. Preparation of capsules

One of the extracts or extract mixtures of Table 7 above, 0.2 mg

100 mg of corn starch

100 mg of milk

Stearic acid  Magnesium 2 mg

After mixing the above components, the capsules were filled in gelatin capsules according to the conventional preparation method of capsules.

4. Manufacture of rings

One of the extracts or extract mixtures of Table 7 above, 0.003 g

Lactose 1.5 g

Glycerin 1 g

Xylitol  0.5 g

After mixing the above components, they were prepared so as to be 4 g per one ring according to a conventional method.

5. Manufacture of granules

2 mg < / RTI > of either the extract or the extract mixture of Table 7 above

Soybean extract 50 mg

200 mg of glucose

600 mg of starch

After mixing the above components, 100 mg of 30% ethanol was added and dried at 60 캜 to form granules, which were then filled in a capsule.

Formulation example  2: Manufacture of cosmetics

1. Manufacture of softening longevity (skin lotion)

As the following composition, the softening longevity was manufactured according to a conventional method.

One of the extracts or extract mixtures of Table 7 above, 0.1%

Beta-1,3-glucan 1.0 wt%

Butylene glycol 2.0 wt%

Propylene glycol 2.0 wt%

Carboxyvinyl polymer 0.1 wt%

0.2% by weight of phage-12 nonylphenyl ether

Polysorbate 80 0.4 wt%

Ethanol 10.0 wt%

0.1% by weight triethanolamine

Preservative 0.05 wt%

0.05% by weight of pigment

0.05% by weight fragrance

Purified water balance

2. Manufacture of nutrition lotion (milk lotion)

A nutritional lotion was prepared according to a conventional method as shown below.

One of the extracts or extract mixtures of Table 7 above, 0.1%

Beta-1,3-glucan 1.0 wt%

4.0 wt%

Polysorbate 60 1.5 wt%

1.5% by weight of sorbitan sesquioleate

0.5% by weight liquid paraffin

Caprylic / capric triglyceride 5.0 wt%

Glycerin 3.0 wt%

3.0% by weight of butylene glycol

3.0% by weight of propylene glycol

Carboxyvinyl polymer 0.1 wt%

0.2% by weight triethanolamine

Preservative 0.05 wt%

0.05% by weight of pigment

0.05% by weight fragrance

Purified water balance

3. Manufacture of nutritional cream

Nutritive creams were prepared according to the usual methods, with the following composition.

One of the extracts or extract mixtures of Table 7 above, 0.2%

Beta-1,3-glucan 5.0 wt%

Wax 10.0 wt%

Polysorbate 60 1.5 wt%

≪ tb > < tb > < tb >

0.5% by weight of sorbitan sesquioleate

Liquid paraffin 10.0 wt%

Squalane 5.0 wt%

Caprylic / capric triglyceride 5.0 wt%

Glycerin 5.0 wt%

3.0% by weight of butylene glycol

3.0% by weight of propylene glycol

0.2% by weight triethanolamine

Preservative 0.05 wt%

0.05% by weight of pigment

0.05% by weight fragrance

Purified water balance

4. Manufacture of massage cream

A massage cream was prepared according to the usual method, with the following composition.

One of the extracts or extract mixtures of Table 7 above, 0.1%

Beta-1,3-glucan 3.0 wt%

Wax 10.0 wt%

Polysorbate 60 1.5 wt%

≪ tb > < tb > < tb >

0.8% by weight of sorbitan sesquioleate

Liquid paraffin 40.0 wt%

Squalane 5.0 wt%

Caprylic / capric triglyceride 4.0 wt%

Glycerin 5.0 wt%

3.0% by weight of butylene glycol

3.0% by weight of propylene glycol

0.2% by weight triethanolamine

Preservative 0.05 wt%

0.05% by weight of pigment

0.05% by weight fragrance

Purified water balance

5. Manufacture of pack

As in the following composition, packs were prepared according to conventional methods.

One of the extracts or extract mixtures of Table 7 above, 0.2%

Beta-1,3-glucan 1.0 wt%

Polyvinyl alcohol 13.0 wt%

0.2% by weight of sodium carboxymethylcellulose,

Glycerin 5.0 wt%

Allantoin 0.1 wt%

6.0% by weight of ethanol

0.3% by weight of phage-12 nonylphenyl ether

Polysorbate 60 0.3 wt%

Preservative 0.05 wt%

0.05% by weight of pigment

0.05% by weight fragrance

Purified water balance

Formulation example  3: Topical  Produce

1. Manufacture of gel

As in the following composition, the gel was prepared by a conventional method.

One of the extracts or extract mixtures of Table 7 above, 0.1%

0.1% by weight of beta-1,3-glucan

0.05% by weight of sodium ethylenediaminetetraacetate

Glycerin 5.0 wt%

Carboxyvinyl polymer 0.3 wt%

5.0% by weight of ethanol

≪ tb > < tb > < tb >

Triethanolamine 0.3 wt%

Preservative 0.05 wt%

0.05% by weight of pigment

0.05% by weight fragrance

Purified water balance

2. Manufacture of ointment

Ointment was prepared according to the usual method, with the following composition.

One of the extracts or extract mixtures of Table 7 above, 0.5%

Beta-1,3-glucan 10.0 wt%

Wax 10.0 wt%

Polysorbate 60 5.0 wt%

≪ tb > < tb > < tb >

0.5% by weight of sorbitan sesquioleate

Vaseline 5.0 wt%

Liquid paraffin 10.0 wt%

Squalane 5.0 wt%

SHARE BUTTER 3.0 wt%

Caprylic / capric triglyceride 5.0 wt%

Glycerin 10.0 wt%

Propylene glycol 10.2 wt%

0.2% by weight triethanolamine

Preservative 0.05 wt%

0.05% by weight of pigment

0.05% by weight fragrance

Purified water balance

3. Preparation of topical medicament (gel ointment)

As the following composition, a gel ointment agent was prepared according to a conventional method.

One of the extracts or extract mixtures of Table 7 above, 0.5%

Beta-1,3-glucan 10.0 wt%

1.5% by weight of polyacrylic acid (Carbopol 940)

5.0% by weight of isopropanol

Hexylene glycol 25.0 wt%

Triethanolamine 1.7 wt%

Deionized water balance

4. Preparation of topical medicines (patches)

As in the following composition, a patch was prepared according to a conventional method.

One of the extracts or extract mixtures of Table 7 above, 0.5%

Beta-1,3-glucan 3.0 wt%

Hexylene glycol 20.0 wt%

Diethylamine 0.7 wt%

1.0% by weight of polyacrylic acid (Carbopol 934P)

0.1% by weight of sodium sulfite

1.0% by weight of polyoxyethylene lauryl ether (E.O. = 9)

1.0% by weight of polyhydroxyethylene cetyl stearyl ether (Cetomacrogol 1000)

Viscous paraffin oil 2.5 wt%

2.5% by weight of caprylic acid ester / capric acid ester (Cetiol LC)

Polyethylene glycol 400 3.0 wt%

Deionized water balance

Formulation example  4: Manufacturing of food

Foods comprising the extract of the present invention, the extract of Coracao root, a mixture thereof, or a mixture of four plant extracts of Nepal plant were prepared as follows.

1. Manufacture of Flour Food

0.05-1.0 parts by weight of one of the extracts or extract mixtures of Table 7 was added to wheat flour, and bread, cake, cookies, crackers and noodles were prepared using the mixture to prepare health foods.

2. Manufacture of dairy products

0.2 part by weight of either the extract or the extract mixture of Table 7 above was added to milk and the milk was used to make a variety of dairy products such as butter and ice cream.

3. Manufacturing of wire

Brown rice, barley, glutinous rice, and yulmu were dried by a known method and dried, and the mixture was granulated to a powder having a particle size of 60 mesh. Black soybeans, black sesame seeds, and perilla seeds were steamed and dried by a known method, and then they were prepared into powders having a particle size of 60 mesh by a grinder. One of the extracts or the mixture of the extracts of Table 7 was concentrated under reduced pressure in a vacuum concentrator, sprayed and dried with a hot air drier, and the resulting dried material was pulverized to a particle size of 60 mesh with a pulverizer to obtain a dry powder.

The grains, seeds, and dry powder of one of the extracts or extract mixtures of Table 7 were prepared by blending 100 parts by weight of the mixed powder in the following proportions.

(30 parts by weight of brown rice, 15 parts by weight of yulmu, 20 parts by weight of barley)

Seeds (7 parts by weight of perilla, 8 parts by weight of black beans, 7 parts by weight of black sesame seeds)

One of the extract or extract mixture of Table 7 (0.1 part by weight),

(0.5 part by weight),

Rhubarb (0.5 Weight portion )

Formulation example  5: Manufacture of beverages

1. Manufacture of health drinks

0.1 mg of one of the extracts or extract mixtures of Table 7 above

Citric acid 1000 mg

100 g of oligosaccharide

Plum concentrate 2 g

Taurine 1 g

Purified water  All 900 mL

The above components were mixed according to a conventional health drink manufacturing method, and the mixture was heated at 85 DEG C for about 1 hour with stirring. Then, the solution thus prepared was filtered and sterilized in a sterilized 2L-container, Lt; RTI ID = 0.0 > health < / RTI >

Although the composition ratio is a mixture of the components suitable for the preferred beverage as a preferred embodiment, the blending ratio may be arbitrarily varied according to the regional and national preferences such as the demand level, the demanding country, and the intended use.

2. Manufacture of vegetable juice

1 g of one of the extracts or extract mixtures of Table 7 of the present invention was added to 1,000 mL of tomato or carrot juice to prepare health promotion vegetable juice.

3. Manufacture of fruit juice

1 g of one of the extracts or extract mixtures of Table 7 was added to 1,000 mL of apple or grape juice to prepare fruit juice for health promotion.

Claims (9)

A cosmetic composition for improving the skin condition containing, as an active ingredient, Timus linalis leaf extract, collagen root extract or a mixture thereof,
Wherein the composition further comprises Winter Beanonia root extract, Principia utiliatus seed extract or a mixture thereof,
Wherein the improvement of the skin condition is skin regeneration, skin elasticity improvement, skin wrinkle prevention or improvement, skin inflammation improvement or skin whitening. A quasi-drug composition for improving the skin condition, which contains, as an active ingredient, a leaf extract of Timus Linneais, a collagen root extract or a mixture thereof,
Wherein the composition further comprises Winter Beanonia root extract, Principia utiliatus seed extract or a mixture thereof,
Wherein the improvement of the skin condition is skin regeneration, skin elasticity improvement, skin wrinkle prevention or improvement, skin inflammation improvement or skin whitening. A composition for improving skin condition, comprising as an active ingredient a leaf extract of Timus Linneais, a collagen root extract or a mixture thereof,
Wherein the composition further comprises Winter Beanonia root extract, Principia utiliatus seed extract or a mixture thereof,
Wherein the skin condition improvement is skin regeneration, skin elasticity improvement, skin wrinkle prevention or improvement, skin inflammation improvement or skin whitening. The present invention relates to a pharmaceutical composition for preventing or treating a skin disease, which contains, as an active ingredient, a leaf extract of Timus rinareis, a collagen root extract or a mixture thereof,
Wherein the composition further comprises Winter Beanonia root extract, Principia utiliatus seed extract or a mixture thereof,
Wherein the skin disease is skin wounds, skin scars, skin inflammation or skin pigmentation. The method of claim 1, wherein the tea leaf extract or a mousse Linea less collaboration car root extract were tea leaves or mousse Linea less collaboration Ka root water, alcohols of C ₁ to C _4, 1,3- butylene glycol and ethyl acetate Wherein the cosmetic composition is obtained by extracting with at least one solvent selected from the group consisting of: delete The method of claim 1, wherein the winter Begonia root extract or print in sepia right subtilis seed extract were frozen begonia roots or print in sepia right subtilis seed water, C ₁ to C ₄ alcohols, 1,3- butylene glycol, and ethyl Acetate, and the like. The cosmetic composition according to claim 1, A cosmetic improvement method of a skin condition comprising administering the cosmetic composition of claim 1 to a subject,
Wherein the improvement of the skin condition is skin regeneration, skin elasticity improvement, skin wrinkle prevention or improvement, skin inflammation improvement or skin whitening. delete