KR20170071899A - Composition for improving skin - Google Patents

Abstract

The present invention relates to a composition for improving skin. The sunflower extract according to the present invention can be used for the production of medicines for the improvement of skin texture and skin whitening, cosmetics or foods through the effect of reducing skin roughness and inhibiting melanin synthesis.

Description

[Composition for improving skin]

The present invention relates to a composition for improving skin showing improvement in skin texture and skin whitening effect.

Skin is the most external organ of the body. It is an important organ that has various functions and greatly affects appearance and image. The skin is composed of various cells and unique structure covering the outer surface of the body. It protects the body against external factors such as evaporation of water, temperature control, external stimulation such as ultraviolet rays, and prevention of harmful microorganisms. .

The skin consists of three layers of epidermis, dermis and subcutaneous fat. The dermis inside the epidermis plays the most important role in terms of aging of the skin, and therefore, it is also an object of intensive research in terms of skin cosmetics.

Constituent fibers that play an important role in maintaining the structure of the dermis include cytokaratin, integrin, laminin, and collagen. These fibrous constituent fibers constitute the skeleton of the dermis originally and play a role in maintaining healthy skin. Double cytokeratin, integrin and laminin exist in the dermal epidermal junction, which connects the epidermis and dermis. It plays a role in regulating the intensity of binding of epidermis and dermis. Collagen is present throughout the dermis, And it is known to play a role in regulating elasticity. Particularly, in the case of collagen, it is known that when the normal structure is not formed, the strength and elasticity of the collagen are considerably weaker than those of the normal type, and they do not help to maintain the structure of the dermis.

Therefore, many studies have been conducted to analyze the effect on the skin by promoting the synthesis of the respective constituent fibers in order to improve the skin texture.

However, in the conventional cosmetic composition, only an increase in the amount of each constituent fiber is confirmed, and no substantial research has been conducted on the effect of improving skin texture. Therefore, there is a need for research on substances that have skin irritation-free effects on skin.

In particular, the skin is an organ that shows a significant change in the age of the human organ, and morphological changes according to this age occur in the stratum corneum, epidermis, and whole dermis constituting the skin. The skin texture, which is the shape of the surface of the skin, is characterized by a three-dimensional microstructure formed by fine lines and is significantly different by age and area. The skin texture is divided into primary (about 20-100 μm), secondary (about 5-40 μm), and tertiary (about 0.5 μm) lines depending on the depth of the line. And it has a distinctive feature of polygons and stars that come from the lines. However, as the age and the skin become damaged, the network structure of the dense fine lines collapses, and the fine lines (secondary, tertiary, etc.) disappear and the primary lines become deeper and wrinkled. In other words, wrinkles, which is a typical symptom of skin aging, is a convalescence of minute changes over a long period of time.

Recently, the study of whitening agents as cosmetics has become increasingly necessary as skin blackness is recognized as skin aging due to ultraviolet rays, along with the improvement of living standards in Asian countries, which prefer emotionally white skin. Accordingly, substances exhibiting tyrosinase inhibitory activity such as ascorbic acid, hydroquinone, glutathione, and arbutin have been used in cosmetics and medicines (Patent Documents 1 and 2) 2) Most of them are ineffective or unstable because of their shape. In particular, a compound such as hydroquinone exhibits a strong decolorizing action, has a skin sensitization itself, can induce skin allergies and the like, and exhibits adverse effects such as inducing vitiligo by changing normal skin functions. Thus, Its use is limited.

In addition, even if the substance inhibits the activity of tyrosinase as described above, if such a substance acts on keratinocytes, which are keratinocytes, to increase the production of factors promoting melanin biosynthesis, the whitening action is consequently weakened The activity inhibitor of tyrosinase is not considered to be a good whitening agent because of its adverse effect, and many tyrosinase inhibitors have been developed, but most of them are known to show no excellent whitening effect.

Under such circumstances, it is urgently required to develop a composition which is safe to the living body, stable in its effective component and, more particularly, has better skin texture and whitening effect than the existing skin texture improving and whitening effect.

On the other hand, sunchae (蓴菜) is the scientific name Brasenia schreberi JF Gmel. It is a perennial search plant belonging to the inhabitant fish harbor (Cabombaceae). It is found in a pond rich in organic matter and a lake with a weak acidity, and it is known that one species is distributed worldwide.

Its appearance is 50-100 cm in height, leaves are 6-10 cm in length, and 4-6 cm in width. The shape is elliptical. The leaf is flat on the surface and the back of the leaf is purple and the petiole is in the center. When the leaves are growing, they are surrounded by a lump with a young stalk. Flowers bloom in June ~ August, reddish in black color, running on axillary leaves, and a little immersed in water. The calyx leaf has a wide rosette shape and has 3 pieces, and the petal has 3 long oval shape. There are many stamens and 6-18 pistils. The fruit is ovate and has calyx and style.

According to the Dong-bo-gyung, it is effective for purulent closure, elimination, clearance and detoxification, and is mainly used for treatment of urine discomfort, species, The representative component is alpha-L-Rhamnopyranosyl- (1-> 3) -alpha-D-galactopyranosyl- (1-> 3) -L-fucose.

Korean Patent Registration No. 10-0923141 Korean Patent Registration No. 10-0921947

 Pengxiao Liu et al., Mechanism of Biological Liquid Superlubricity of Brasenia schreberi Mucilage, Langmuir, Vol. 30, Pages 3811-3816, 2014  Hyunsook Kim et al., Polysaccharide gel coating of the leaves of Brasenia schreberi lowers plasma cholesterol in hamsters, Journal of Traditional and Complementary Medicine, Vol. 5, Pages 56-61, 2015

Thus, the inventors of the present invention have completed the present invention by confirming that the mulberry extract reduces the roughness of the skin to exhibit a skin texture improving effect and inhibit melanin synthesis to exhibit a whitening effect.

Accordingly, it is an object of the present invention to provide a composition for improving skin texture and whitening skin, which comprises a snack extract as an active ingredient.

As a means for solving the above problems, the present invention provides a composition for improving skin texture and whitening skin, which comprises, as an active ingredient, a snack extract for the production of medicines, cosmetics or foods.

The sunflower extract according to the present invention can be used for the production of medicines, cosmetics or foods by alleviating the roughness of the skin to exhibit the skin texture improving effect and inhibiting melanin synthesis to exhibit whitening effect.

Fig. 1 shows the result of measuring the skin roughness parameter Rmax (maximum height) according to the net extract.
FIG. 2 shows the results of measurement of the skin roughness parameter Rz (average roughness) according to the safflower extract.

Hereinafter, the configuration of the present invention will be described in detail.

In order to exert an excellent effect when applied to actual skin, improvement of skin texture and skin whitening activity are exhibited at a low concentration, and the ability to permeate and absorb the skin is excellent. It is preferable that the composition is low in volatility so that it can stay for a sufficient time to exhibit a whitening effect and that the active ingredient remains stable on the composition or on the skin and is easily prepared into medicines, cosmetics or foods and is safe for the skin. However, the components satisfying all of the above-mentioned characteristics among the known components are not common. For example, some skin tone improvement and skin whitening ingredients are excellent in skin tone improvement and skin whitening activity even at a low concentration in an in vitro test, but they are difficult to be applied to real skin because of their ability to be absorbed through the skin. Other active ingredients have low hydrophilicity, making them difficult to formulate into medicines, cosmetics, and foods. In addition, some skin tone improvement and skin whitening ingredients may be degraded or transformed into other compounds when they are exposed to heat, light, or oxygen, and the effects may already be lost before application to the skin.

As can be seen in the following examples, the whole herbal extract has excellent skin texture improving effect and melanin formation inhibitory effect at a low concentration, and thus can be used as an effective ingredient of medicines, cosmetics or food compositions for improving skin texture and whitening skin .

Accordingly, the present invention provides a composition for improving skin texture and skin whitening comprising an active ingredient as an effective ingredient.

In the past, it has been a part of waterfowl that has grown up in our country, but now it is part of the marsh area of Halla Mountain (the largest native area in Korea), a clean area of Jeju Island. The juvenile leaves are covered with slippery mucilaginous surface. Unlike normal mucin, this mucilage does not wash off well and keeps mucinous even in water. It is excellent in hydrophilicity and moisture contained in mucinous juice easily escapes There are features that do not. The mucosubstance component of the sweet potato is a type of long-chain polysaccharide linked with a monosaccharide and is composed of monosaccharides such as L-arabinose, L-fucose, D-glucuronic acid and D-mannose have.

The safflower extract can be extracted by a method known in the art, and the method is not particularly limited. Alternatively, commercially available extracts can be used.

Preferably, the safflower extract may be an extract obtained by extracting the whole dish with water and / or an organic solvent. The organic solvent may be a polar organic solvent, a nonpolar organic solvent or a mixture thereof. The polar organic solvent may be a lower alcohol having 1 to 5 carbon atoms, ethyl acetate or acetone, and the nonpolar organic solvent may be ether, chloroform, benzene, hexane or dichloromethane. For example, the lower alcohol having 1 to 5 carbon atoms may be methanol, ethanol, propanol, butanol or isopropanol.

In one embodiment, the snuff extract may include a fraction obtained by fractionating the primary extract extracted using the above-described extraction solvent with an extraction solvent having a different polarity. For example, the mulberry extract may be a fraction obtained by extracting with an alcohol having 1 to 5 carbon atoms and then fractionating again with a solvent having a different polarity such as ether, benzene or hexane. Two or more kinds of solvents may be used for the fractionation, and the solvent extracts may be sequentially used or mixed according to the polarity of the solvent.

According to one embodiment of the present invention, in order to obtain a polysaccharide at a high ratio from the net, the whole leaves are immersed in primary distilled water at about 20 ° C for 12 hours to 24 hours to remove impurities and 100 parts by weight of impurities Were removed, and the mixture was immersed at a temperature of about 80 캜 for 2 hours to 4 hours to prepare an immersion liquid. After the soaked product was pressurized, it was filtered to isolate the whole leaf and the mucous lyophilic part, and 300 parts of ethanol or more was added to 100 parts of the mucilage of the separated pure mushroom to isolate only the polysaccharide of the net. The separated polysaccharide was washed with ethanol, and 1 part by weight of the purified polysaccharide was dissolved in 99 parts by weight of the third distilled water to prepare a 1% pure polysaccharide extract.

In the present invention, the extracted extract or the fraction obtained by performing the fractionation process may be filtered, concentrated or dried to remove the solvent, and the filtration, concentration, and drying may all be performed. Specifically, the filtration can be performed using a filter paper or a vacuum filter, and the concentration can be reduced by using a reduced pressure concentrator or the like, and the freeze-drying method can be carried out for drying.

The fractions obtained by performing the above-described extract or the fractionation process can be further purified by silica gel column chromatography, thin layer chromatography or high performance liquid chromatography Further purification can be achieved by purification using various chromatographic techniques.

The compositions of the present invention may be used for the manufacture of pharmaceutical formulations.

The pharmaceutical formulations may be in the form of solutions, suspensions or emulsions in oils or aqueous media, or in the form of excipients, powders, granules, tablets or capsules.

In addition, the composition may further contain one or more active ingredients showing the same or similar functions. For example, it may contain known skin texture improvement and skin whitening ingredients. The addition of additional skin texture improvement and skin whitening ingredients may further improve the skin texture and skin whitening effect of the composition of the present invention. When the above ingredients are added, skin safety, easiness of formulation, and stability of effective ingredients can be considered according to the combined use. In one embodiment of the present invention, the composition is a whitening ingredient known in the art and includes substances inhibiting tyrosinase enzyme activity such as kojic acid and arbutin, hydroquinone, vitamin C (L-Ascorbic acid), derivatives thereof, and various plant extracts. The additional ingredients may be included in an amount of 0.0001 to 10% by weight based on the total weight of the composition, and the content range may be adjusted according to requirements such as skin safety, easiness of formulation of the snack extract.

In addition, the composition of the present invention may further comprise a pharmaceutically acceptable carrier.

Pharmaceutically acceptable carriers may contain a variety of ingredients such as buffer, injectable sterile water, normal saline or phosphate buffered saline, sucrose, histidine, salts and polysorbates, and the like.

The composition of the present invention can be administered orally or parenterally, and can be administered in the form of a general pharmaceutical preparation, for example, various forms of oral and parenteral administration at the time of clinical administration. In the case of formulation, a filler, , A binder, a wetting agent, a disintegrant, a surfactant, and the like.

Solid formulations for oral administration include tablets, pills, powders, granules, capsules and the like. These solid preparations can be prepared by mixing the pharmaceutical composition of the present invention with at least one excipient such as starch, calcium carbonate, Sucrose, lactose, gelatin and the like can be prepared.

In addition to simple excipients, lubricants such as magnesium stearate talc are also used. Examples of liquid formulations for oral administration include suspensions, solutions, emulsions, syrups and the like. Various excipients such as wetting agents, sweeteners, fragrances, preservatives and the like may be included in addition to water and liquid paraffin, which are commonly used simple diluents.

Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. Propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like can be used as the non-aqueous solvent and suspension agent. Examples of the suppository base include witepsol, macrogol, tween 61, cacao butter, laurin, glycerogelatin and the like.

In the present invention, the term " skin texture improving effect " refers to smoothing the skin surface, imparting gloss, and brightening the skin tone by inhibiting or inhibiting roughness of the skin surface due to aging, stress, .

In the present invention, the 'whitening effect' refers to not only brightening the skin tone by inhibiting the synthesis of the melanin pigment but also improving skin hypercholesterolemia due to ultraviolet rays, hormones or heredity, such as spots or freckles.

The composition of the present invention can provide a desirable skin texture improvement and skin whitening effect when an effective amount of the net extract is contained. In the present invention, the term "effective amount" means the amount of the extract capable of exhibiting a whitening effect or improving skin texture. The effective amount of the snack extract contained in the composition of the present invention will vary depending on the form of the composition, the method of applying the extract to the skin, and the length of time on the skin. For example, when the composition is commercialized as a pharmaceutical formulation, it may contain the nettening extract at a higher concentration than the case where it is commercialized as cosmetics that are routinely applied to the skin. Accordingly, the daily dose is 0.1 to 100 mg / kg, preferably 30 to 80 mg / kg, more preferably 50 to 60 mg / kg, and 1 to 6 times / day, ≪ / RTI >

The composition of the present invention may be used alone or in combination with methods using surgery, radiation therapy, hormone therapy, chemotherapy, and biological response modifiers.

The pharmaceutical formulations of the present invention may include external preparation for skin.

In the case of using the safflower extract as an external preparation for skin, it may further comprise at least one selected from the group consisting of fatty substances, organic solvents, solubilizers, thickening and gelling agents, softening agents, antioxidants, suspending agents, stabilizing agents, foaming agents, , Ionic or nonionic emulsifiers, fillers, sequestering and chelating agents, preservatives, vitamins, barrier agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or external preparations for skin ≪ / RTI > any other ingredients that are commonly used in the skin sciences. The components can also be introduced in amounts commonly used in the field of dermatology.

When the sunflower extract is provided as an external preparation for skin, it may have a formulation such as, but not limited to, ointments, patches, gels, creams or sprays.

In addition, the composition for improving skin texture and skin whitening of the present invention can be used for the production of cosmetic formulations.

The cosmetic formulation may be in the form of a conventional emulsion formulation and a solubilized formulation. For example, creams, essences, cosmetic creams, sprays, gels, packs, sunscreens, make-up bases, liquids such as lotions such as lotion, facial lotion, body lotion, A powder, a cleansing lotion, a makeup removing agent such as a cleansing oil, a cleaning agent such as a cleansing foam, a soap, a body wash and the like.

In addition, the cosmetic product may further contain, in addition to the sweet pea extract, a fat substance, an organic solvent, a solubilizing agent, a thickening agent and a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizer, a foaming agent, a fragrance, Or any other conventionally used in cosmetics such as nonionic emulsifiers, fillers, sequestering and chelating agents, preservatives, vitamins, barrier agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, ≪ RTI ID = 0.0 > ingredients. ≪ / RTI >

The cosmetic formulation may contain a relatively high concentration of the net extract in the case of a wash-off type cosmetic such as a make-up remover, a cleanser, etc. in which the effective ingredient remains on the skin in a short period of time. On the other hand, in the case of leave-on type cosmetics such as cosmetics, milky lotions, creams, and essences whose effective ingredients stay on the skin for a long period of time, It will be acceptable. In one embodiment of the present invention, but not limited thereto, the composition may comprise 0.001% to 20% by weight (preferably 0.001% to 1% by weight) of the nettening extract based on the total weight of the composition . When the composition of the present invention contains less than 0.001% by weight of the snack extract, sufficient skin texture and skin whitening effect can not be expected. When the composition contains more than 20% by weight, undesired reactions such as allergy may occur, This is to prevent safety problems.

In addition, the skin tone improving and skin whitening compositions of the present invention can be used for the production of food formulations.

The food formulation refers to a food prepared by adding the snack extract to a food material such as beverage, tea, spice, gum, confectionery, or the like, or by encapsulation, powdering, suspension or the like.

Since the food formulation can be ingested on a daily basis, high skin texture improvement and skin whitening effect can be expected, which is very useful.

In the case of using the safflower extract as a food additive, the safflower extract may be directly added or used together with other food or food ingredients, and may be suitably used according to a conventional method. The amount of the active ingredient to be mixed can be suitably determined according to its intended use (prevention, health or therapeutic treatment). Generally, the composition of the present invention is added in an amount of not more than 15 parts by weight, preferably not more than 10 parts by weight, based on the raw material, when the food or beverage is produced. However, in the case of long-term consumption intended for health and hygiene purposes or for health control purposes, the amount may be less than the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount exceeding the above range .

There is no particular limitation on the kind of the food. Examples of the food to which the above substance can be added include dairy products including meat, sausage, bread, chocolate, candy, snack, confectionery, pizza, ramen, other noodles, gums, ice cream, various soups, drinks, tea, Alcoholic beverages, and vitamin complexes, all of which include health foods in a conventional sense.

When the food is a beverage, various flavors or natural carbohydrates may be added as an additional ingredient such as a normal drink. The above-mentioned natural carbohydrates are sugar alcohols such as monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, polysaccharides such as dextrin and cyclodextrin, and xylitol, sorbitol and erythritol. Examples of sweeteners include natural sweeteners such as tau martin and stevia extract, synthetic sweeteners such as saccharin and aspartame, and the like. The ratio of the natural carbohydrate is generally about 0.01 to 0.04 g, preferably about 0.02 to 0.03 g per 100 mL of the composition of the present invention.

In addition to the above, the food formulations may contain various nutrients, vitamins, electrolytes, flavors, colorants, pectic acid and salts thereof, alginic acid and its salts, organic acids, protective colloid thickeners, pH adjusters, stabilizers, preservatives, glycerin, And the like. Other food formulations may contain flesh for the production of natural fruit juices, fruit juice drinks and vegetable drinks. These components may be used independently or in combination. The proportion of such additives is not critical, but is generally selected in the range of 0.01 to 0.1 parts by weight per 100 parts by weight of the composition of the present invention.

Hereinafter, the present invention will be described in detail with reference to examples. However, the following examples are illustrative of the present invention, and the present invention is not limited to the following examples.

Example  1: Containing snack extract Cosmetics  Preparation of composition

Brasenia schreberi) by adding about 20 5 1 L of deionized water to about 500g immersed for 12 to 24 hours at room temperature to remove the impurities in the sunchae. The first washed distilled water was removed from the sieve by using the first distilled water. 500 mL of distilled water was added to 500 g of the drained net, and the mixture was heated at a temperature of about 80 for 2 hours to 4 hours. After heating, the mixture was allowed to cool to room temperature, and then the mixture was cooled down and filtered to obtain 721.2 g of a sweet potato extract. The thus obtained snack extract was used as a cosmetic composition in the following experimental examples.

Example  2: a solution containing a net polysaccharide aqueous solution Cosmetics  Preparation of composition

About 2 L of ethanol was mixed and mixed well to the net snack extract of Example 1, and only the net polysaccharide was precipitated and filtered. The filtered polysaccharide was washed with ethanol to obtain pure pure polysaccharide (1.39 g). Using the components shown in Table 1 below, the purified polysaccharide was added to the third distilled water and dissolved for 24 to 48 hours to prepare a 1% pure polysaccharide aqueous solution. The 1% solution of the net polysaccharide thus obtained was used as a cosmetic composition in the following experimental examples.

Component of cosmetic composition content Net polysaccharide 1g Distilled water 99g Total amount 100g

Experimental Example  1. First skin irritation test ( A closed pile  exam)

Experiments were conducted to examine the skin irritation of the cosmetic composition prepared according to Examples 1 and 2 above. (0.2 g) of each sample was applied to the back region of a healthy adult male and female for 24 hours, and the pin chamber was removed. After 4 hours, the change of the skin condition was visually observed, Table 2 shows the results. The degree of irritation was calculated by the following equation. In Experimental Example 1, distilled water was used as a non-treated control, and SLS (sodium lauryl sulfate) was used as a positive control.

[Mathematical Expression]

division Tested embroidery Judgment result Irritation degree ++ + + - - Distilled water
(Negative control) 50 people - One 3 46 0.10 SLS 0.1%
(Positive control group) One 2 7 40 0.28 Example 1 - One 6 43 0.16 Example 2 - One 4 45 0.12 -: No erythema or no abnormality
+ -: slightly reddish from the surroundings
+: Significantly reddened than surrounding
++: More reddening and swelling than the surrounding area

As shown in Table 2, it was confirmed that the composition according to the present invention was not irritating to the skin and was harmless to the human body.

Experimental Example  2: Skin texture  Evaluation of improvement efficacy

The skin-improving effect of the above Examples 1 and 2 was evaluated as follows. Twenty two women in their 40s and 50s were allowed to apply the test product twice a day for 6 weeks to the entire face. The skin roughness of the cheek area was photographed and analyzed using a 3-D 3D Imaging System (PRIMOS PICO) at 3 weeks (3W) and 6 weeks (6W) at each evaluation point. The skin roughness parameter Rmax (maximum height) and the parameter Rz (average roughness) were measured. The skin roughness parameter Rmax (maximum height) was obtained by taking the surface of the reference length as long as the distance between two parallel lines contacting the highest mountain and the deepest bone , The parameter Rz (average roughness) is calculated by drawing a line (parallel to the line) parallel to the average line of the cross-sectional curve and calculating the difference between the average value of the distance from the baseline of the highest 5 mountains and the average value of the distance from the baseline of the lowest 5 , And the Rmax value and the Rz value indicate a larger value as the roughness increases. The results are shown in Fig. 1 and Fig. Fig. 1 shows the measurement result of the parameter Rmax (maximum height), and Fig. 2 shows the measurement result of the parameter Rz (average roughness).

According to Figs. 1 and 2, the skin texture of Examples 1 and 2 was significantly improved compared with that before use, and the improvement rate was higher than that of distilled water (control group) at all evaluation points (3 and 6 weeks). In particular, in Example 2, it was confirmed that the skin-rupture parameter Rmax value decreased by 6.14% and Rz value by 6.32% after 6 weeks of use, compared with before use.

Experimental Example  3: Whitening effect - evaluation of inhibitory effect on melanin formation

Examples 1 and 2 were added to the culture of mouse melanoma cells (B-16 mouse melanoma cell) to determine the whitening effect by measuring the total amount of melanin at the cellular level (Lotan R., Lotan D., Cancer Res. 40: 3345-3350, 1980).

To evaluate the toxicity of melanoma cells in rats before the experiment, whitening evaluation was performed by selecting a concentration that is not toxic. Examples 1 and 2 were diluted to 1/100 and 1/1000, respectively, in the culture medium using cell culture medium to give final concentrations of 10 ppm and 100 ppm, respectively. The control group, arbutin, was added to the medium to a concentration of 100 ppm, And then cultured for 3 days. Then, the cells were treated with trypsin, removed from the culture vessel, centrifuged, and extracted with melanin. The removed cells were incubated with 1 mL of 1 N sodium hydroxide solution, boiled for 10 minutes to dissolve melanin, and the absorbance was measured at 400 nm using a spectrophotometer to determine the amount of melanin produced. The amount of melanin was measured by an absorbance of 1 × 10 ⁶ cells per unit cell, and the total amount of melanin production relative to the control group was calculated as an inhibition rate (%). The results are summarized in Table 3 below.

Inhibitory effect of melanin formation at the cellular level (number of repeats = 3) sample Melanin production (Abs) Inhibition rate (%) Example 1 (100 ppm) 0.223 32.42 Example 1 (10 ppm) 0.260 21.21 Example 2 (100 ppm) 0.219 33.64 Example 2 (10 ppm) 0.251 23.94 Positive control (arbutin, 100 ppm) 0.228 30.91 Negative control (DMSO, 10 ppm) 0.33 -

As can be seen from the results of Table 3, it can be confirmed that Examples 1 and 2 exhibit excellent whitening effect compared to arbutin, which is a positive control group, at the same concentration (100 ppm). Also, it can be confirmed that the amounts of melanin produced in Examples 1 and 2 having a concentration of 10 ppm are also remarkably high even when the control group is compared. Therefore, it was confirmed that the whitening effect was improved in proportion to the treatment concentration by the decrease of the amount of melanin production as the treatment concentration was increased.

Hereinafter, formulation examples of the composition containing the extract of the present invention will be described, but the present invention is not intended to be limited thereto but is specifically described.

Formulation example  1: Manufacture of pharmaceutical preparations

1. Preparation of tablets

Sunflower extract 0.2 mg

100 mg of corn starch

100 mg of milk

2 mg of magnesium stearate

After mixing the above components, tablets were prepared by tableting according to a conventional method for producing tablets.

Formulation example  2: Manufacture of cosmetics

1. Manufacture of nutritional cream

As shown in the following composition, a nutritional cream containing an orange peel extract as an active ingredient was prepared according to a conventional method.

The net weight

Beta-1,3-glucan 5.0 wt%

Wax 10.0 wt%

Polysorbate 60 1.5 wt%

≪ tb > < tb > < tb >

0.5% by weight of sorbitan sesquioleate

Liquid paraffin 10.0 wt%

Squalane 5.0 wt%

Caprylic / capric triglyceride 5.0 wt%

Glycerin 5.0 wt%

3.0% by weight of butylene glycol

3.0% by weight of propylene glycol

0.2% by weight triethanolamine

Preservative 0.05 wt%

0.05% by weight of pigment

0.05% by weight fragrance

Purified water to 100%

Formulation example  3: Preparation of external preparation for skin

1. Manufacture of ointment

As shown in the following composition, an ointment containing an orange juice extract as an active ingredient was prepared according to a conventional method.

Suyuchae extract 0.5 wt%

Beta-1,3-glucan 10.0 wt%

Wax 10.0 wt%

Polysorbate 60 5.0 wt%

≪ tb > < tb > < tb >

0.5% by weight of sorbitan sesquioleate

Vaseline 5.0 wt%

Liquid paraffin 10.0 wt%

Squalane 5.0 wt%

SHARE BUTTER 3.0 wt%

Caprylic / capric triglyceride 5.0 wt%

Glycerin 10.0 wt%

Propylene glycol 10.2 wt%

0.2% by weight triethanolamine

Preservative 0.05 wt%

0.05% by weight of pigment

0.05% by weight fragrance

Purified water to 100%

Claims (10)

A composition for improving skin texture, comprising as an active ingredient a mulberry extract.
The method according to claim 1,
The composition for improving skin texture according to claim 1, wherein the extract is a mixture of at least one selected from the group consisting of water and an organic solvent.
3. The method of claim 2,
The organic solvent may be a polar solvent containing a lower alcohol having 1 to 5 carbon atoms, ethyl acetate, or acetone; A nonpolar solvent comprising ether, chloroform, benzene, hexane or dichlorohexane; Or a mixed solvent thereof.
The method according to claim 1,
The herbal extract of the present invention has a content of 0.001% by weight to 20% by weight based on the total weight of the composition.
The method according to claim 1,
Wherein the composition is for the manufacture of a medicament for the improvement of skin texture, a cosmetic preparation or a food product.
The method according to claim 1,
The composition for improving skin texture according to claim 1, wherein the safflower extract is a polysaccharide.
A whitening composition for skin whitening, comprising as an active ingredient, a mulberry extract.
8. The method of claim 7,
Wherein the composition is for skin whitening medicines, cosmetics or foods.
8. The method of claim 7,
The whitening extract of claim 1, which contains 0.001% to 20% by weight of the composition.
8. The method of claim 7,
Wherein said nettening extract is a polysaccharide.

Images