KR20170025365A

KR20170025365A - Composition for improving skin

Info

Publication number: KR20170025365A
Application number: KR1020150121706A
Authority: KR
Inventors: 여혜린; 김도형; 김효진; 이상화
Original assignee: 주식회사 엘지생활건강
Priority date: 2015-08-28
Filing date: 2015-08-28
Publication date: 2017-03-08

Abstract

The present invention relates to a composition for improving skin condition. According to the present invention, a Paeoniae Radix Rubra extract shows effects of brightening skin tone and promoting proliferation of skin stem cells, and thus can be used for producing foods, cosmetic ingredients, or drugs for improving skin textures and regenerating skin. Additionally, the Paeoniae Radix Rubra extract also promotes proliferation of skin stem cells and maintains stemness properties, thereby being useful for producing cosmetic ingredients for promoting activities of stem cells.

Description

[Composition for improving skin]

The present invention relates to a skin improving composition exhibiting skin regeneration, skin texture improvement, and stem cell activity promoting effect.

The skin texture, which is the shape of the skin surface, is characterized by a three-dimensional microstructure formed by fine lines and is significantly different by age and area. The skin texture is divided into primary (about 20-100 μm), secondary (about 5-40 μm), and tertiary (about 0.5 μm) lines depending on the depth of the line. And has a characteristic that the polygons and star formation that occur when the lines meet meet [non-patent document 1].

However, as the age increases or the skin becomes damaged, the network structure of the dense fine lines collapses and fine lines (secondary, tertiary, etc.) disappear and the primary lines become deeper and wrinkles are known to be produced Literature 2, 3]. In other words, wrinkles, a typical sign of skin aging, are accumulations of minute changes that have already occurred over a long period of time.

Embryologically, all components of human skin are known to originate from ectoderm or mesodermal lobe. Epidermis, hair follicles, sebaceous glands and glands are originated from ectoderm, and melanocytes, nerves and special sensory receptors are derived from neuroepithelial cells. According to the developmental stage, the embryonic stem cells are repeatedly differentiated and become cells with the characteristics of each tissue function. After the embryo, a certain number of stem cells remain in the tissue. . The first is in the hair follicle. It is known to play an important role in the regeneration of the epidermis into cells before cell differentiation occurs, and plays an important role in hair regeneration and growth. The second is the basal layer of the epidermis. The stem cells found here play an important role in maintaining skin health by administering not only the epidermis but also the fibroblasts of the dermal layer. The stem cells here are relatively large in quantity and easy to obtain, making them widely used in the study of skin stem cells. The skin is constantly renewed, and stem cells present in the epithelium of the skin, that is, epidermal stem cells of the skin, are involved in the repair of the epithelium after injury [Non-Patent Document 4]. The epithelial stem cells of the skin are also referred to as skin stem cells. When the skin stem cells are activated, it is possible to treat skin wounds such as trauma, promote wound healing, have. Integrin β1 and integrin α6 are used as indicators of dermal stem cells, and maintenance of skin stem cells necessary for epithelial morphogenesis and differentiation has been reported to be regulated by p63 protein.

Dermal stem cells play an important role in maintaining the health and physiological and biochemical homeostasis of the skin. Stem cells present in the skin are also abnormally functioned due to the effect of aging, and thus various problems arise as the homeostasis of the skin is broken. Therefore, various phenomena of skin aging can be improved through the activation of stem cells [Non-Patent Document 5].

In addition, it is urgently required to develop a composition which is safe to the living body, has a stable active ingredient, and most of all has a skin regeneration and skin texture improving activity, which is superior to the existing skin regeneration and skin texture improving effect.

On the other hand, 작작작약 赤赤赤赤赤赤赤赤赤赤赤赤赤赤赤赤赤赤赤赤赤赤赤赤赤赤............................................................................................................................................................................................................................................ Flowers are mainly used for gardening, and roots are used as medicine for pain, stomach pain, dysmenorrhea, amenorrhea, hematemesis, anemia, bruise. The roots contain Paeoniflorin, Paeonol, Paeonin, Benzoic acid, essential oils, tannins, β-sistol and triterpenoids. It has been reported that there is inhibitory action on stomach and intestinal smooth muscle and uterine smooth muscle, and suppression of gastric secretion, hypoglycemia, diuretic, analgesic, paralysis, epilepsy and hematopoiesis.

Techniques related to cosmetics using peony fungi include a method of preparing a cosmetic soap using a peony seed extract (Korean Patent No. 10-0804993) and an antioxidant and moisturizing detergent composition (Korean Patent Laid-Open Publication No. 2005-0118819) However, there have been no studies on stem cells.

Journal of the European Academy of Dermatology and Venereology. 12: 103-114, 1999 The British journal of dermatology. 110: 129-138, 1984 Skin research and technology.5: 189-194, 1999 Epidermal Stem Cells of the Skin, Cedric Blanpain, Elaine Fuchs, Annual Review of Cell and Developmental Biology, November 2006, Vol. 22, Pages 339-373 Human skin stem cells and the aging process, Catherine Niemann, Stem Cell Aging and Regenerative Medicine, November 2008, Pages 986-997

Accordingly, the present inventors have found that an extract of a herbal medicine promotes the proliferation of skin stem cells, promotes skin regeneration, promotes proliferation of skin stem cells as well as skin texture improving effect due to improvement in skin brightness, exhibits a stem cell- And confirming the stimulatory effect on stem cell activity, thereby completing the present invention.

Accordingly, an object of the present invention is to provide a composition for improving skin regeneration and skin texture, which comprises an effective drug extract as an active ingredient.

Another object of the present invention is to provide a composition for stimulating stem cell activity, which comprises the following active ingredient extract as an active ingredient.

As a means for solving the above problems, the present invention provides a composition for skin regeneration and skin texture improvement, which comprises an effective drug extract for the manufacture of medicines, cosmetics or foods, as an active ingredient.

As another means for solving the above-mentioned problems, the present invention provides a composition for promoting stem cell activity comprising an effective drug extract as an active ingredient for the production of cosmetic formulations.

The extract of the present invention can be used for the production of medicines, cosmetics or foods by promoting the proliferation of skin stem cells and promoting the regeneration of skin and improving the skin texture due to the improvement of skin brightness.

In addition, the extract of the herb extract promotes the proliferation of dermal stem cells and exhibits a stem cell-retaining effect, and thus can be used for the production of cosmetic formulations for promoting stem cell activity.

Hereinafter, the configuration of the present invention will be described in detail.

In order to exert an excellent effect when the skin regeneration and skin texture improving ingredient is applied to the actual skin, it exhibits highly active skin regeneration and skin texture improving activity at a low concentration, has excellent ability to permeate and absorb the skin, It is preferable to have a low volatility so as to be able to stay for a sufficient time to exhibit the improvement effect, to keep the active ingredient stable on the composition or the skin, to be easily produced into medicines, cosmetics or foods, and to be safe for the skin. However, the components satisfying all of the above-mentioned characteristics among the known components are not common. For example, some skin regeneration and skin texture improving agents have excellent skin regeneration and skin texture improving activity even at low concentrations in vitro, but they are difficult to apply to real skin because of their ability to permeate through skin. Other active ingredients have low hydrophilicity, making them difficult to formulate into medicines, cosmetics, and foods. In addition, some skin regeneration and skin regeneration components may be decomposed or transformed into other compounds when they are exposed to heat, light, or oxygen, and the effect may already be lost before application to the skin.

As can be seen in the following examples, the extract of P. edulis shows remarkably excellent stem cell proliferation promoting effect and skin brightness improvement effect at a low concentration, and thus can be used as an effective ingredient of medicines, cosmetics or food compositions for skin regeneration and skin texture improvement have.

Accordingly, the present invention provides a composition for skin regeneration and skin texture improvement comprising an effective drug extract as an active ingredient.

In the present invention, it is also called root of Paeonia genus lactiflora (= Paeonia albiflora), veitchii and the like, and it is also referred to as a herb medicine, a red pepper, a red pepper, a green cedar, a rhododendron, a cauliflower,

The extract of the herbal medicine may be extracted by a method known in the art, and the method is not particularly limited. Alternatively, commercially available extracts can be used.

The extract may be a flower, leaf, branch, fruit, root, stem, seed or a mixed extract thereof.

Preferably, the extract of the present invention may be an extract obtained by extracting an effective amount of an anti-depressant with water and / or an organic solvent. The organic solvent may be a polar organic solvent, a non-polar organic solvent or a mixture thereof. The polar organic solvent may be a lower alcohol having 1 to 5 carbon atoms, ethyl acetate or acetone, and the nonpolar organic solvent may be ether, chloroform, benzene, hexane or dichloromethane. For example, the lower alcohol having 1 to 5 carbon atoms may be methanol, ethanol, propanol, butanol or isopropanol.

In one embodiment, the crude drug extract may include a fraction obtained by fractionating the primary extract extracted using the above-described extraction solvent with an extraction solvent having a different polarity. For example, the extract may be a fraction obtained by extracting with an alcohol having 1 to 5 carbon atoms and then fractionating again with a solvent having a different polarity such as ether, benzene or hexane. Two or more kinds of solvents may be used for the fractionation, and the solvent extracts may be sequentially used or mixed according to the polarity of the solvent.

In the present invention, the extracted extract or the fraction obtained by performing the fractionation process may be filtered, concentrated or dried to remove the solvent, and the filtration, concentration, and drying may all be performed. Specifically, the filtration can be performed using a filter paper or a vacuum filter, and the concentration can be reduced by using a reduced pressure concentrator or the like, and the freeze-drying method can be carried out for drying.

The fractions obtained by performing the above-described extract or the fractionation process can be further purified by silica gel column chromatography, thin layer chromatography or high performance liquid chromatography Further purification can be achieved by purification using various chromatographic techniques.

The compositions of the present invention may be used for the manufacture of pharmaceutical formulations.

The pharmaceutical formulations may be in the form of solutions, suspensions or emulsions in oils or aqueous media, or in the form of excipients, powders, granules, tablets or capsules.

In addition, the composition may further contain one or more active ingredients showing the same or similar functions. For example, it may contain known skin regeneration and skin tone improving ingredients. The skin regeneration and skin texture improving effect of the composition of the present invention may be further enhanced. When the above ingredients are added, skin safety, easiness of formulation, and stability of effective ingredients can be considered according to the combined use. In one embodiment of the present invention, the composition is a skin regeneration component known in the art comprising retinoic acid, TGF, protein from animal placenta, betulinic acid and chlorella extract, antioxidant components known in the art, such as tocopherol, selenium , Vitamin C and phenolic compounds, whitening ingredients known in the art, substances inhibiting the activity of tyrosinase enzymes such as kojic acid and arbutin, hydroquinone, vitamin C (L- Ascorbic acid, derivatives thereof, and various plant extracts. The additional ingredient may be contained in an amount of 0.0001% by weight to 10% by weight based on the total weight of the composition, and the content range may be adjusted according to requirements such as skin safety and easiness in formulation of the extract of the present invention.

In addition, the composition of the present invention may further comprise a pharmaceutically acceptable carrier.

Pharmaceutically acceptable carriers may contain a variety of ingredients such as buffer, injectable sterile water, normal saline or phosphate buffered saline, sucrose, histidine, salts and polysorbates, and the like.

The composition of the present invention can be administered orally or parenterally, and can be administered in the form of a general pharmaceutical preparation, for example, various forms of oral and parenteral administration at the time of clinical administration. In the case of formulation, a filler, , A binder, a wetting agent, a disintegrant, a surfactant, and the like.

Solid formulations for oral administration include tablets, pills, powders, granules, capsules and the like. These solid preparations can be prepared by mixing the pharmaceutical composition of the present invention with at least one excipient such as starch, calcium carbonate, Sucrose, lactose, gelatin and the like can be prepared.

In addition to simple excipients, lubricants such as magnesium stearate talc are also used. Examples of liquid formulations for oral administration include suspensions, solutions, emulsions, syrups and the like. Various excipients such as wetting agents, sweeteners, fragrances, preservatives and the like may be included in addition to water and liquid paraffin, which are simple diluents commonly used.

Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. Propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like can be used as the non-aqueous solvent and suspension agent. Examples of the suppository base include witepsol, macrogol, tween 61, cacao butter, laurin, glycerogelatin and the like.

In the present invention, the term 'skin regeneration effect' refers to the recovery of skin tissue against damage caused by external or internal causes of skin as the activity of skin stem cells is promoted. At this time, the damage caused by the external cause may include ultraviolet rays, external pollutants, wound or trauma, and the damage caused by the internal cause may be stress.

In the present invention, the term " skin texture improving effect " refers to smoothing the skin surface, imparting gloss, and brightening the skin tone by inhibiting or inhibiting roughness of the skin surface due to aging, stress, .

In the present invention, the term 'effective amount' means the amount of the extract capable of promoting regeneration of damaged skin or improving skin texture. When the composition of the present invention contains an effective amount of the extract of the present invention, it is possible to provide a skin regenerating effect and a skin texture improving effect. The effective amount of the extract of the present invention contained in the composition of the present invention will vary depending on the form in which the composition is commercialized, the method in which the compound is applied to the skin, and the time on the skin. For example, when the composition is commercialized as a pharmaceutical formulation, it may contain the extract of the present invention in a higher concentration as compared with the case where it is commercialized as cosmetics to be applied to skin on a daily basis. Accordingly, the daily dosage is 0.1 to 100 mg / kg, preferably 30 to 80 mg / kg, more preferably 50 to 60 mg / kg, and 1 to 6 times / day, &Lt; / RTI >

The composition of the present invention may be used alone or in combination with methods using surgery, radiation therapy, hormone therapy, chemotherapy, and biological response modifiers.

The pharmaceutical formulations of the present invention may include external preparation for skin.

When the extract of the present invention is used as an external preparation for skin, it may further contain at least one selected from the group consisting of fatty substances, organic solvents, solubilizers, thickening and gelling agents, softening agents, antioxidants, suspending agents, stabilizing agents, foaming agents, , Ionic or nonionic emulsifiers, fillers, sequestering and chelating agents, preservatives, vitamins, barrier agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or external preparations for skin &Lt; / RTI > any other ingredients that are commonly used in the skin sciences. The components can also be introduced in amounts commonly used in the field of dermatology.

When the drug extract is provided as an external preparation for skin, it may have a formulation such as, but not limited to, ointments, patches, gels, creams or sprays.

In addition, the composition for improving skin regeneration and skin texture of the present invention can be used for the production of cosmetic formulations.

The cosmetic formulation may be in the form of a conventional emulsion formulation and a solubilized formulation. For example, creams, essences, cosmetic creams, sprays, gels, packs, sunscreens, make-up bases, liquids such as lotions such as lotion, facial lotion, body lotion, A powder, a cleansing lotion, a makeup removing agent such as a cleansing oil, a cleansing foam, a soap, a body wash, and the like.

The cosmetic composition may further contain, in addition to the active ingredient extract, a lipid, an organic solvent, a solubilizer, a thickening agent and a gelling agent, a softener, an antioxidant, a suspending agent, a stabilizer, a foaming agent, a perfume, Or any other conventionally used in cosmetics such as nonionic emulsifiers, fillers, sequestering and chelating agents, preservatives, vitamins, barrier agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, &Lt; RTI ID = 0.0 > ingredients. &Lt; / RTI >

The cosmetic formulation may contain a relatively high concentration of the extract of the present invention in the case of a wash-off type cosmetic such as a make-up remover, a cleanser, etc. in which the active ingredient remains on the skin in a short period of time. On the other hand, in the case of leave-on type cosmetics such as lotion, cream, essence and the like in which the active ingredient remains on the skin for a long period of time, It will be acceptable. In one embodiment of the present invention, but not limited thereto, the composition may contain 0.0001 wt% to 10 wt% (preferably 0.0001 wt% to 1 wt%) of the extract of the present invention on the total weight of the composition . When the composition of the present invention contains less than 0.0001% by weight of the extract of the present invention, sufficient skin regeneration and skin texture improving effect can not be expected. When the composition contains more than 10% by weight, undesired reactions such as allergy occur, This is to prevent safety problems.

In addition, the composition for skin regeneration and skin texture improvement of the present invention can be used for manufacturing food formulations.

The food formulation refers to a food prepared by adding the extract of the present invention to a food material such as beverage, tea, spices, gum, confectionery, or the like, or by encapsulation, powdering, suspension or the like.

Since the food formulation can be ingested on a daily basis, high skin regeneration and skin texture improving effect can be expected, which is very useful.

In case of using the extract of the present invention as a food additive, the extract of the present invention may be added as it is or may be used together with other food or food ingredients, and may be suitably used according to a conventional method. The amount of the active ingredient to be mixed can be suitably determined according to its intended use (prevention, health or therapeutic treatment). Generally, the composition of the present invention is added in an amount of not more than 15 parts by weight, preferably not more than 10 parts by weight, based on the raw material, when the food or beverage is produced. However, in the case of long-term consumption intended for health and hygiene purposes or for health control purposes, the amount may be less than the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount exceeding the above range .

There is no particular limitation on the kind of the food. Examples of foods to which the above substances can be added include dairy products including meats, sausages, breads, chocolates, candies, snacks, confections, pizza, ramen noodles, gums, ice cream, soups, drinks, tea, Alcoholic beverages, and vitamin complexes, all of which include health foods in a conventional sense.

When the food is a beverage, various flavors or natural carbohydrates may be added as an additional ingredient such as a normal drink. The above-mentioned natural carbohydrates are sugar alcohols such as monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, polysaccharides such as dextrin and cyclodextrin, and xylitol, sorbitol and erythritol. Examples of sweeteners include natural sweeteners such as tau martin and stevia extract, synthetic sweeteners such as saccharin and aspartame, and the like. The ratio of the natural carbohydrate is generally about 0.01 to 0.04 g, preferably about 0.02 to 0.03 g per 100 mL of the composition of the present invention.

In addition to the above, the food formulations may contain various nutrients, vitamins, electrolytes, flavors, colorants, pectic acid and salts thereof, alginic acid and its salts, organic acids, protective colloid thickeners, pH adjusters, stabilizers, preservatives, glycerin, And the like. Other food formulations may contain flesh for the production of natural fruit juices, fruit juice drinks and vegetable drinks. These components may be used independently or in combination. The proportion of such additives is not critical, but is generally selected in the range of 0.01 to 0.1 parts by weight per 100 parts by weight of the composition of the present invention.

The present invention also provides a composition for stimulating stem cell activity, comprising the above extract of the present invention as an active ingredient.

The term 'stem cell' in the present invention is a cell capable of cell division by itself and capable of differentiating into a very specific type of specific cell type. The type of such stem cells is not particularly limited, and in one embodiment, the stem cells may be dermal stem cells. The term 'dermal stem cells' refers to stem cells that can be differentiated into cells constituting the skin (epidermis, dermis and subcutaneous fat layer). The cells that make up the skin include keratinocytes, melanocytes, and fibroblasts (mainly responsible for biosynthesis of collagen and elastin) present in the epidermis.

The kind of the skin stem cell is not particularly limited. The dermal stem cells used in the present invention can be used irrespective of where they originate from. For example, dermal stem cells may be obtained from a known source of dermal stem cells, e. G., From the basal layer of hair follicles or epidermis, and the animal to be harvested may be a mammal. In one embodiment, the mammal may include, but is not limited to, a human, a mouse, a rat, a guinea pig, a rabbit, a monkey, a pig, a horse, a cattle, a sheep, Preferably the mammal can be human. Such methods of obtaining dermal stem cells from dermal stem cell sources are well known in the art.

In the present invention, the term 'promoting effect on stem cell activity' refers to an effect of promoting stem cell proliferation and / or an effect of maintaining stem cell characteristics, which is a specific indicator molecule and a self-cleaving property of stem cells.

In addition, the compositions of the present invention can be used for the production of cosmetic formulations. These cosmetic formulations are the same as those described in the description of the cosmetic formulation containing the active ingredient extract.

Hereinafter, the present invention will be described in detail with reference to examples. However, the following examples are illustrative of the present invention, and the present invention is not limited to the following examples.

Manufacturing example 1: Preparation of extract

The roots of the herbal medicine were washed and powdered. After cooling for 5 days in a 10-fold amount of ethanol solvent, the extract solution was filtered and concentrated to obtain an extract. To this was added a mixed solution of purified water and ethanol to dissolve and then filtered to prepare a final extract.

Manufacturing example 2: Serum-free Under medium conditions Of dermal stem cells culture

Human epidermal stem cells purchased from Cellntec were added to 48-well plates (6 × 10 ³ cells / well), supplemented with BPE (bovine pituitary extract) similar to fetal bovine serum And cultured for 24 hours at 5% CO ₂ and 37 ° C using CNT-57 medium (Cellntec). Subsequently, the culture medium was removed through a suction tube, and then the medium was removed using PBS solution (GibcoBRL). 5 urn of CO ₂ and 37% of CNT-57 medium containing no BPE were treated with 5 ug / Lt; 0 > C for 72 hours.

Experimental Example One: CCK -8 Through evaluation method Dermal stem cells Proliferation promoting effect

The skin stem cells cultured in Reference Example 2 were evaluated for CCK-8 (Cell counting kit-8). The CCK-8 assay is an indirect method of measuring the density of living cells by measuring the absorbance of Formazan, a dehydrogenase in the intracellular electron transport system, produced by the decomposition of tetrazolium salt.

Cells were spectrophotometrically measured by treating CCK-8 solution (treated with 1/10 of the medium volume) at 37 ° C for 2 hours and then measuring the absorbance at 450 nm. The proliferation rate (%) of the stem cell extract of the present invention was determined from the measured absorbance value in accordance with the following equation (1) with respect to the control group (CNT-57 medium supplemented with BPE), and the results are shown in Table 1 below.

[Equation 1]

Growth rate (%) = (absorbance of sample-treated group / absorbance of control group) x 100

Promoting the proliferation of dermal stem cells Additive sample density Cell proliferation (%) versus control An effective drug extract 5 ug / ml 159% Control group - 100%

As shown in Table 1, the skin stem cells under the medium conditions treated with the extract of the herb extract showed excellent growth promoting effect.

Experimental Example 2: Of dermal stem cells Stem cell ( stemness ) Confirm maintenance effect

The expression level of p63 was evaluated in order to confirm the stem cell maintenance effect of the dermal stem cells cultured in Reference Example 2 above. p63 is an intracellular transcription factor and is well known as a maintenance marker for dermal stem cells. Cells were isolated and cDNA was synthesized. Then, real-PCR was performed with Taqman staining solution to measure the expression level of p63. The concentration of RNA in this experiment was normalized to S16 ribosomal RNA.

The experimental results are shown in Table 2 below. In Table 2 below, the numerical value of the increase in p63 is a multiple of the control group (CNT-57 medium supplemented with BPE).

Stem cell (stem cell) maintenance effect (number of repeats = 3) Additive sample density p63 Incremental multiple (times) An effective drug extract 5 / / mL 2.32 Control group - 1.0

As shown in Table 2, the medium conditions treated with the extract of P. aeruginosa stimulated the expression of p63 in dermal stem cells, thereby exhibiting excellent stem cell retention.

Example 1: For the improvement of the turn-over of the crust Efficacy

About the nutritional cream of the following formulation example 2, the effect of the improvement of the exfoliation of the skin in the healthy twenties to 50 women was tested as follows.

Twenty of 20 women aged 20 to 50 years were given a 1.5% solution of DHA (Dihydroxyacetone, Sigma Aldrich, USA) for 8 hours, followed by deposition. The preparation cream was applied twice daily to the test site. After 4 days of application, 8 days after application, 12 days after application, photographs were taken using instrument Chromameter CR-400 (Minolta, Japan) and skin lightness of DSLR and DSLR. The measured values were evaluated by three mean values excluding the maximum value and the minimum value. The higher the improvement in the skin brightness of the deposition site, the better the improvement of the exfoliation. The results are shown in Table 3 below.

Skin brightness improvement effect division Four days after application Eight days after application After 12 days of application Improvement rate (%) 1.78 4.76 15.35

As shown in Table 3, when the nutritional cream according to the present invention was used, it was found that the exfoliation of the skin was improved.

Formulation example 1: Manufacture of pharmaceutical preparations

1. Preparation of tablets

0.2 mg

100 mg of corn starch

100 mg of milk

2 mg of magnesium stearate

After mixing the above components, tablets were prepared by tableting according to a conventional method for producing tablets.

Formulation example 2: Manufacture of cosmetics

1. Manufacture of nutritional cream

As shown in the following composition, a nutritional cream containing an effective drug extract as an active ingredient was prepared according to a conventional method.

0.2%

Beta-1,3-glucan 5.0 wt%

Wax 10.0 wt%

Polysorbate 60 1.5 wt%

&Lt; tb > < tb > < tb >

0.5% by weight of sorbitan sesquioleate

Liquid paraffin 10.0 wt%

Squalane 5.0 wt%

Caprylic / capric triglyceride 5.0 wt%

Glycerin 5.0 wt%

3.0% by weight of butylene glycol

3.0% by weight of propylene glycol

0.2% by weight triethanolamine

Preservative 0.05 wt%

0.05% by weight of pigment

0.05% by weight fragrance

Purified water to 100%

Formulation example 3: Preparation of external preparation for skin

1. Manufacture of ointment

As shown in the following composition, an ointment containing an effective drug extract as an active ingredient was prepared according to a conventional method.

0.5%

Beta-1,3-glucan 10.0 wt%

Wax 10.0 wt%

Polysorbate 60 5.0 wt%

&Lt; tb > < tb > < tb >

0.5% by weight of sorbitan sesquioleate

Vaseline 5.0 wt%

Liquid paraffin 10.0 wt%

Squalane 5.0 wt%

SHARE BUTTER 3.0 wt%

Caprylic / capric triglyceride 5.0 wt%

Glycerin 10.0 wt%

Propylene glycol 10.2 wt%

0.2% by weight triethanolamine

Preservative 0.05 wt%

0.05% by weight of pigment

0.05% by weight fragrance

Purified water to 100%

Claims

A composition for regenerating skin comprising an effective drug extract as an active ingredient.

The method according to claim 1,
The composition for skin regeneration is an extract obtained by extracting an effective medicament with at least one selected from the group consisting of water and an organic solvent.

3. The method of claim 2,
The organic solvent may be a polar solvent containing a lower alcohol having 1 to 5 carbon atoms, ethyl acetate, or acetone; A nonpolar solvent comprising ether, chloroform, benzene, hexane or dichlorohexane; Or a mixed solvent thereof.

The method according to claim 1,
Wherein the composition is for the manufacture of a medicament for skin regeneration, a cosmetic preparation or a food product.

A composition for improving skin texture comprising an effective drug extract as an active ingredient.

6. The method of claim 5,
Wherein the composition is for the manufacture of a medicament for the improvement of skin texture, a cosmetic preparation or a food product.

A composition for stimulating stem cell activity comprising an effective drug extract as an active ingredient.

8. The method of claim 7,
Wherein the composition is for promoting stem cell proliferation or stem cell retention.

8. The method of claim 7,
Wherein the composition is for the production of a cosmetic for promoting stem cell activity.