KR20170035659A - Composition for improving skin comprising perivine as active ingredient - Google Patents

Composition for improving skin comprising perivine as active ingredient Download PDF

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KR20170035659A
KR20170035659A KR1020150134841A KR20150134841A KR20170035659A KR 20170035659 A KR20170035659 A KR 20170035659A KR 1020150134841 A KR1020150134841 A KR 1020150134841A KR 20150134841 A KR20150134841 A KR 20150134841A KR 20170035659 A KR20170035659 A KR 20170035659A
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skin
composition
active ingredient
effect
improving
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KR1020150134841A
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Korean (ko)
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김효진
김도형
이상화
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주식회사 엘지생활건강
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
    • A61K31/4353—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom ortho- or peri-condensed with heterocyclic ring systems
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2200/00—Function of food ingredients
    • A23V2200/30—Foods, ingredients or supplements having a functional effect on health
    • A23V2200/318—Foods, ingredients or supplements having a functional effect on health having an effect on skin health and hair or coat

Abstract

The present invention relates to a skin improving composition. Perivine according to the present invention can be used in producing a pharmaceutical, cosmetic, food, or skin external application agent formulation for regenerating skin, alleviating wrinkles, improving elasticity, whitening skin, alleviating atopic dermatitis, moisturizing skin, improving skin textures, and reinforcing skin barriers through effects in promoting a skin stem cell activity, promoting the collagen synthesis of skin fibroblasts, promoting melanin synthesis, removing free radicals, ensuring an excellent activity of PPAR alpha, improving the expression of filaggrin, and improving the brightness of skin. Moreover, the perivine promotes the proliferation of skin stem cells, and has an effect in maintaining stem cell properties, thereby being able to be used in producing a cosmetic formulation for promoting a stem cell activity.

Description

[0001] The present invention relates to a composition for improving skin comprising peribin as an active ingredient,

The present invention relates to a skin improving composition exhibiting skin regeneration, wrinkle improvement, elasticity enhancement, skin whitening, antioxidation, atopic improvement, skin moisturizing, skin texture improvement and stem cell activity promoting effect.

Collagen is a major substrate protein produced in fibroblasts of the skin and exists in extracellular epilepsy. Its important functions are mechanical rigidity of skin, resistance of connective tissues and binding force of tissues, support of cell adhesion, division of cells and differentiation Growth or wound healing) are known. Such collagen is reduced by aging and photo aging caused by ultraviolet irradiation, which is known to be closely related to the wrinkling of the skin. Also, in recent years, extensive research on skin aging has developed, and important functions of collagen in skin have been revealed.

Effective ingredients promoting collagen synthesis and exhibiting wrinkle-reducing effects are known. For example, retinoic acid, transforming growth factor (TGF) [non-patent document 1], animal placenta-derived protein [Patent document 1], betulinic acid [Patent document 2], chlorella extract [ Patent Documents 3 and 4] are known as collagen synthesis promoting substances. However, the above-mentioned effective ingredients are limited in the use amount due to safety problems such as irritation and redness when applied to the skin, or have insufficient effect, so that the effect of improving the skin function by promoting the collagen synthesis of the skin can not be expected.

On the other hand, active oxygen introduced from the outside of the living body or generated in the living body causes many problems such as promoting aging of the living body, cancer, and the like. Therefore, the development and research of antioxidants that inhibit oxidation by active oxygen have been performed. Antioxidants are widely distributed in copper and plants. Many phenolic compounds, flavonoids, tocopherols, vitamin C and selenium are known in fruits and vegetables. However, the antioxidant substances present in nature can not be expected to have practically sufficient effects in skin application. Therefore, although synthetic antioxidants having excellent antioxidant ability and low cost are widely used, their use is restricted due to safety concerns such as human side effects.

In addition, the epidermis located at the outermost part of the skin protects against various external physical, chemical and mechanical stimuli and protects against excessive divergence of body water through the skin. This protective function is possible by normally forming and maintaining the stratum corneum composed of keratinocytes. The keratinocyte is a cell formed by a stepwise change in morphology and function while a basal cell that continuously proliferates in the stratum basale moves to the stratum corneum, Forming cells are removed from the skin and the new keratinocytes from the epidermis's bottom layer repeat the process of epidermis differentiation or keratinization replacing its function. In this keratinization process, keratinocytes produce intercellular lipids such as natural moisturizing factors (NMF) and ceramides, cholesterol, and fatty acids, which act as barrier layers to the outside, As shown in Fig.

In addition, skin dryness, which is considered to be one of the major diseases of modern society, is one of the symptoms caused by skin barrier function abnormality. Recently, environmental pollution, increase in dry environment such as apartments and high rise building, increase in social stress, Of excessive bathing, skin aging, and the like, and the cases of severe symptoms and the need for treatment are also increasing steadily.

Atopic dermatitis, which is present in 10% of children in recent years, is also known to be a cause of dry skin syndrome, and more fundamentally, abnormal skin barrier function. In order to treat such atopic dermatitis, studies have been carried out in order to supply water from the outside or minimize water loss from the body by focusing on proper moisture retention in the skin in the past. Actually, ceramide or derivatives thereof have been developed and are widely used in the pharmaceutical or cosmetic field.

However, the use of such a moisturizing agent is not only a fundamental treatment but a temporary symptom relief, and thus has not shown sufficient effect for treatment of dry skin and skin barrier function including atopic dermatitis. Therefore, it is urgent to develop a substance that can restore the damaged skin barrier fundamentally.

On the other hand, peroxisome proliferation-activated receptors (hereinafter referred to as PPARs) are ligand-inducible transcription factors belonging to the nuclear hormone receptor superfamily, including cell differentiation and proliferation, lipid homeostasis and energy metabolism, It regulates many cellular and metabolic processes. To date, three PPAR subfamilies, PPARα (NR1C1), PPARδ (NR1C2, also known as PPARβ, FAAR and NUC1) and PPARγ (NR1C3) have been identified. The receptor forms a dimer with retinoid X receptor (RXR) and regulates gene expression by binding to a specific base sequence called PPRE (PPAR response elements) present in the regulatory region of the target gene.

These PPARs are found throughout the skin constituting cells and play an important role in the maintenance and restoration of the skin barrier function, the moisturizing ability, and the expression of the inflammatory healing process. In other words, PPAR is a factor regulating energy homeostasis. Especially, it is known that PPAR is involved in regulation of skin condition such as control of permeability of skin barrier, inhibition of epidermal growth, induction of differentiation of epidermal layer through various mechanisms. Because of this characteristic, PPAR is known to be expressed abundantly in keratinocytes and dermal fibroblasts, which constitute the skin tissue. It is known that PPAR is expressed in atopic dermatitis, inflammation related skin disease, psoriasis, wound healing, It is known that it acts as a key regulator of a variety of skin diseases.

Recently, it has been known that when PPAR alpha present in keratinocytes is activated by binding to its ligand, it promotes the differentiation of keratinocytes and reconstructs damaged skin barrier. In the case of applying Clofibrate (non-patent document 2) or WY 14643 (non-patent document 3), which is an agonist of known PPAR alpha, to the skin of which skin barrier is damaged, differentiation of keratinocytes is promoted And recovery of the skin barrier is promoted.

Filament aggregating protein (filaggrin) is a protein that plays an important role in the formation of the stratum corneum along with proteins such as keratin, involucrin, and loricrin. It is mainly involved in the formation of filaments by binding with keratin, and is processed in the form of filaggrin and natural moisturizing factor (NMF) from profilaggrin in the stratum corneum production process. Ultimately, it forms a natural moisturizing factor, which is 20-30% (based on dry weight) of the stratum corneum, and is excellent in the ability to absorb moisture, thus acting as a humectant to control the moisture of the stratum corneum. Moisture content in these stratum corneum is essential for normal activity of various enzymes important for stratum corneum formation.

Therefore, the reduction of the expression level of pilar green causes changes in the function and moisturization of the skin barrier and leads to various lesions. In particular, the formation of normal skin barrier plays an important role in defense against external stimuli, and when this function is lost, it plays a key role in the progression of atopy. In addition, the absorption of antigens through the skin is an important cause of the increase in atopic dermatitis, and pilar green is the target of effective atopic dermatitis improvement.

In addition, it is the desire of everyone to have a whiter skin. It is genetically determined by the concentration and distribution of melanin in human skin, but is also influenced by environmental or physiological conditions such as sunlight, fatigue, and stress. Melanin is produced by a nonenzymatic oxidation reaction after tyrosine, an amino acid, is converted to DOPA or dopaquinone by an enzyme called tyrosinase. Thus, the pathway through which melanin is made is known, but the mechanism by which melanin synthesis, the previous step in which tyrosinase acts, is not yet elucidated.

On the other hand, commonly known whitening ingredients include substances inhibiting the activity of tyrosinase enzymes such as kojic acid and arbutin, hydroquinone, vitamin C (L-Ascorbic acid) There are plant extracts. By inhibiting the synthesis of melanin pigment, they can brighten the skin tone to realize skin whitening, and it is possible to improve skin hypercholesterolemia such as stain or freckles due to ultraviolet rays, hormones or heredity. However, when applied to skin, there is a problem that the use amount is limited due to safety problems such as irritation and redness, or the effect is insignificant so that a substantial effect can not be expected.

In addition, the skin texture, which is the shape of the skin surface, is characterized by a three-dimensional microstructure formed by microscopic lines and is significantly different according to age and area. The skin texture is divided into primary (about 20-100 μm), secondary (about 5-40 μm), and tertiary (about 0.5 μm) lines depending on the depth of the line. And has a distinctive polygonal shape and star formation due to the contact of the lines [Non-Patent Document 4].

However, as the age increases or the skin becomes damaged, the network structure of the dense fine lines collapses and fine lines (secondary, tertiary, etc.) disappear and the primary lines become deeper and wrinkles are known to be produced Literature 5]. In other words, wrinkles, a typical sign of skin aging, are accumulations of minute changes that have already occurred over a long period of time.

Embryologically, all components of human skin are known to originate from ectoderm or mesodermal lobe. Epidermis, hair follicles, sebaceous glands and glands are originated from ectoderm, and melanocytes, nerves and special sensory receptors are derived from neuroepithelial cells. According to the developmental stage, the embryonic stem cells are repeatedly differentiated and become cells with the characteristics of each tissue function. After the embryo, a certain number of stem cells remain in the tissue. . The first is in the hair follicle. It is known to play an important role in the regeneration of the epidermis into cells before cell differentiation occurs, and plays an important role in hair regeneration and growth. The second is the basal layer of the epidermis. The stem cells found here play an important role in maintaining skin health by administering not only the epidermis but also the fibroblasts of the dermal layer. The stem cells here are relatively large in quantity and easy to obtain, making them widely used in the study of skin stem cells. The skin is constantly renewed, and stem cells present in the epithelium of the skin, that is, epidermal stem cells of the skin, are involved in the repair of the epithelium after injury [Non-Patent Document 6]. The epithelial stem cells of the skin are also referred to as skin stem cells. When the skin stem cells are activated, it is possible to treat skin wounds such as trauma, promote wound healing, have. Integrin β1 and integrin α6 have been used as indices of dermal stem cells and maintenance of dermal stemness necessary for epithelial morphogenesis and differentiation has been reported to be regulated by p63 protein .

Dermal stem cells play an important role in maintaining the health and physiological and biochemical homeostasis of the skin. Stem cells present in the skin are also abnormally functioned due to the effect of aging, and thus various problems arise as the homeostasis of the skin is broken. Therefore, various phenomena of skin aging can be improved through the activation of stem cells [Non-Patent Document 7].

In addition, it is more effective than a substance which is safe in the living body, stable in its active ingredient, and most of which has the effect of regenerating the existing skin, improving wrinkles, improving elasticity, skin whitening, antioxidation, atopy, skin moisturizing, Development of ingredients having excellent skin regeneration, wrinkle improvement, elasticity enhancement, skin whitening, antioxidation, atopic improvement, skin moisturizing, skin texture improvement and skin barrier enhancing activity is urgently required.

1. Japanese Patent Publication No. 8-231370 2. Japanese Patent Publication No. 8-208424 3. Japan Pyeonghwa 9-40523 4. Japan Patent No. 10-36283

1. Cardinale G. et al, Adv. Enzymol., 41, p. 425, 1974 2. Feingold et al., J. Invest. Dermatol., 110, pp 368-375, 1998. 3. Feingold et al., J. Invest. Dermatol., 115, pp 353-360, 2000. 4. Journal of the European Academy of Dermatology and Venereology. 12: 103-114, 1999 5. The British journal of dermatology. 110: 129-138, 1984, Skin research and technology. 5: 189-194, 1999 6. Epidermal Stem Cells of the Skin, Cedric Blanpain, Elaine Fuchs, Annual Review of Cell and Developmental Biology, November 2006, Vol. 22, Pages 339-373 7. Human skin stem cells and the aging process, Catherin Niemann, Stem Cell Aging and Regenerative Medicine, November 2008, Pages 986-997

Accordingly, the present inventors have found that Perivine promotes the proliferation of dermal stem cells, maintains stem cell characteristics, exhibits skin regeneration promoting effect, promotes collagen synthesis of fibroblasts of skin, and improves skin wrinkles or elasticity It exhibits whitening effect by suppressing melanin synthesis, exhibits antioxidative effect by eliminating free radicals, promotes activity of PPAR alpha to exhibit atopic improvement effect, increases expression of filar green to show skin moisturizing effect, Promoting the activation of the alpha activity and enhancing the skin barrier through the increase of the expression of filla green and promoting the proliferation of the skin stem cells and promoting the stem cell activity promoting the stem cell activity, The present invention has been completed.

Accordingly, an object of the present invention is to provide a skin regeneration, wrinkle improvement, elasticity enhancement, skin whitening, antioxidation, atopic improvement, skin moisturizing, skin texture improvement, skin barrier enhancement Which comprises:

[Chemical Formula 1]

Figure pat00001

Another object of the present invention is to provide a composition for stimulating stem cell activity, which comprises, as an active ingredient, a peribin compound represented by the following formula (1)

[Chemical Formula 1]

Figure pat00002

As a means for solving the above-mentioned problems, the present invention provides a method for skin regeneration, wrinkle-improving, anti-wrinkle, anti-wrinkle, anti- Skin whitening, antioxidation, atopic improvement, skin moisturizing, skin texture improvement and skin barrier enhancement.

[Chemical Formula 1]

Figure pat00003

As another means for solving the above problems, the present invention provides a composition for stimulating stem cell activity comprising, as an active ingredient, felibin, which is a compound represented by the following formula (1), for producing a cosmetic formulation:

[Chemical Formula 1]

Figure pat00004

Perivine according to the present invention promotes proliferation of dermal stem cells, maintains stem cell characteristics, exhibits skin regeneration promoting effect, promotes collagen synthesis of fibroblasts of the skin, exhibits skin wrinkle improvement or elasticity enhancing effect , Exhibits whitening effect by inhibiting melanin synthesis, exhibits antioxidative effect by eliminating free radicals, promotes activity of PPAR alpha to exhibit atopy improvement effect, increases expression of filar green to show skin moisturizing effect, and PPAR alpha Enhancing activity of the skin barrier by enhancing activity and increasing the expression of pillar green and exhibiting an effect of improving the skin texture due to the improvement of skin brightness and thus can be used in the manufacture of pharmaceuticals, cosmetics, food or skin external preparation.

In addition, ferbine promotes the proliferation of stem cells and promotes stem cell activity to maintain stem cell activity, and thus can be used in the production of cosmetic formulations for promoting stem cell activity.

Hereinafter, the configuration of the present invention will be described in detail.

Skin regeneration, wrinkle improvement, elasticity enhancement, skin whitening, antioxidation, atopic improvement, skin moisturizing, skin texture improvement and skin barrier enhancement. , Skin whitening, antioxidation, atopic improvement, skin moisturizing, skin texture improvement and skin barrier strengthening activity, and exhibits excellent ability to be absorbed through the skin and is excellent in skin regeneration, wrinkle improvement, elasticity enhancement, skin whitening, , Is low in volatility so as to be able to stay for a sufficient time to exhibit atopic improvement, skin moisturizing, skin texture improvement and skin barrier strengthening effect, and the active ingredient is stably maintained on the composition or skin, Is easy to formulate, and it is preferable that it is safe for the skin. However, the components satisfying all of the above-mentioned characteristics among the known components are not common. For example, some skin regeneration, wrinkle improvement, elasticity enhancement, skin whitening, antioxidation, atopic improvement, skin moisturization, skin texture improvement and skin barrier enhancement components may be used for skin regeneration, wrinkle improvement, It is excellent in whitening, antioxidation, atopic improvement, skin moisturizing, skin texture improvement and skin barrier strengthening activity, but it is difficult to apply to real skin because of its ability to permeate through skin. In addition, some of the ingredients for skin regeneration, wrinkle improvement, elasticity enhancement, skin whitening, antioxidation, atopic improvement, skin moisturizing, skin tone improvement and skin barrier enhancement may be decomposed when the active ingredient is exposed to heat, light, , And the effect may disappear before it is applied to the skin.

As can be seen in the following examples, Perivine is superior to low-concentration skin stem cell activation promoting effect, collagen synthesis promotion effect, melanin formation inhibition effect, antioxidative effect, PPAR alpha activity effect, It can be used as an effective ingredient for pharmacy, cosmetics, food, skin external agent for skin regeneration, wrinkle improvement, elasticity enhancement, skin whitening, antioxidation, atopy improvement, skin moisturization, skin texture improvement and skin barrier enhancement .

Accordingly, the present invention relates to a pharmaceutical composition for skin regeneration, wrinkle improvement, elasticity enhancement, skin whitening, antioxidation, atopic improvement, skin moisturizing, skin barrier improvement and skin barrier strengthening Lt; / RTI >

[Chemical Formula 1]

Figure pat00005

The name of the compound of formula 1 is 4-Demethyl-3-oxovobasan-17-oic acid methyl ester.

The peribaine may be synthesized, or a commercially available compound may be used.

In the present invention, the term " skin regeneration effect " refers to restoration of skin tissue against damage caused by external or internal causes of the skin. Damage due to external causes may include ultraviolet rays, external contaminants, wound, trauma, etc. The damage caused by the internal causes may be stress. Preferably, the skin regeneration is associated with dermal stem cells which play an important role in maintaining the health and physiological and biochemical homeostasis of the skin. The maintenance of the skin stem cell state necessary for epithelial morphogenesis and differentiation is regulated by the p63 protein do. Accordingly, by treating the active ingredient of the present invention, the skin regeneration effect can be remarkably enhanced by promoting the proliferation of the dermal stem cells and maintaining the dermal cellularity.

In the present invention, the term " wrinkle-reducing effect " refers to inhibiting or inhibiting the generation of wrinkles on the skin, or alleviating already-generated wrinkles.

In the present invention, the 'elasticity-enhancing effect' refers to an increase in elasticity to the skin, which suppresses or inhibits the loss of elasticity of the skin, or alleviates the already-reduced elasticity.

Preferably, the wrinkle-improving and elasticity-enhancing effects are related to the mechanical rigidity of the skin, the resistance of the connective tissue and the binding force of the tissue, the support of cell adhesion, and the collagen inducing cell division and differentiation. Therefore, by treating the active ingredient of the present invention, collagen synthesis can be promoted and the effect of improving wrinkles and improving the elasticity can be remarkably improved.

In the present invention, the 'whitening effect' refers to not only brightening the skin tone by inhibiting the synthesis of the melanin pigment but also improving skin hypercholesterolemia due to ultraviolet rays, hormones or heredity, such as spots or freckles.

In the present invention, the term 'antioxidative effect' refers to the action of free radicals or reactive oxygen species (ROS), which are highly reactive according to oxidative stress caused by intracellular metabolism or ultraviolet rays, Refers to inhibition of oxidation, and includes removal of free radical or reactive oxygen species, thereby reducing damage to the cells.

In the present invention, the term 'atopic improvement effect' refers to the effect of preventing or treating skin diseases such as atopic dermatitis, dry skin disease, and suppressing, inhibiting or alleviating atopic symptoms. Preferably, the atopic improvement effect is related to PPAR which plays an important role in the maintenance and restoration of the skin barrier function, the moisturizing ability, and the expression of the inflammatory healing process. Accordingly, by treating the active ingredient of the present invention, the activity of PPAR alpha, a key regulator of atopic dermatitis, can be enhanced to control the skin conditions such as the permeability control of skin barrier, the inhibition of epidermal growth and the induction of differentiation, .

In the present invention, the term 'moisturizing effect' refers to inhibiting or reducing the reduction of moisture in the skin, or increasing the moisture content of the skin to smooth and smooth the surface of the skin. Preferably, the moisturizing effect is related to filaggrin, a protein that plays an important role in the formation of the stratum corneum, and the decrease in the expression amount of filagreen changes the function of skin barrier and skin moisturization. Accordingly, by treating the active ingredient of the present invention, the expression of pillared green associated with skin moisturization can be promoted, and skin moisturizing effect can be remarkably improved.

In the present invention, the term " skin texture improving effect " refers to smoothing the skin surface, imparting gloss, and brightening the skin tone by inhibiting or inhibiting roughness of the skin surface due to aging, stress, .

In the present invention, the 'skin barrier' is a skin barrier that performs a defense function against various physical, chemical and mechanical stimuli of the outside. The horny layer formed by repeated epidermal differentiation or keratinization acts as a barrier layer against the outside, It means to function. Accordingly, by treating the active ingredient of the present invention, the activity of PPAR alpha is increased and the differentiation of keratinocytes of the epidermal layer is increased, and the expression level of filagreen protein, which is a moisturizing factor, is increased to make the surface of the skin healthy. Reinforcing effect '.

Compositions for skin regeneration, wrinkle improvement, elasticity enhancement, skin whitening, antioxidation, atopic improvement, skin moisturizing, skin texture improvement and skin barrier enhancement containing the above-mentioned fibrin as an active ingredient of the present invention can be used for the production of cosmetic formulations .

The cosmetic formulations may be prepared in the form of conventional emulsified formulations and solubilized formulations. For example, creams, essences, cosmetic creams, sprays, gels, packs, sunscreens, make-up bases, liquids such as lotions such as lotion, facial lotion, body lotion, A powder, a cleansing lotion, a makeup removing agent such as a cleansing oil, a cleansing foam, a soap, a body wash, and the like.

In addition, the cosmetic may further comprise, in addition to the peribaine, a lipid, an organic solvent, a solubilizing agent, a thickening and gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizing agent, a foaming agent, a fragrance, As used herein means any emulsion or emulsion which is commonly used in emulsifying or emulsifying agents, fillers, sequestering and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or cosmetics Adjuvants commonly used in the cosmetics field, such as other ingredients.

The cosmetic formulation may contain a relatively high concentration of the ferry bean in the case of a wash-off type cosmetic such as a make-up remover, a detergent, etc. in which the active ingredient remains on the skin in a short period of time. On the other hand, in the case of leave-on type cosmetics such as lotion, cream, essence and the like in which the active ingredient remains on the skin for a long period of time, It will be acceptable. In one embodiment of the present invention, but not limited thereto, the composition may comprise 0.0001% to 10% by weight (preferably 0.0001% to 1% by weight) of the feriin relative to the total composition weight . When the composition of the present invention contains less than 0.0001% by weight of the above-mentioned fibrin, sufficient skin regeneration, wrinkle improvement, elasticity enhancement, skin whitening, antioxidation, atopic improvement, skin moisturizing and skin texture improving effect can not be expected, %, It is intended to prevent such an unpleasant reaction such as allergies or a problem of skin safety.

Also, the composition for improving skin regeneration, wrinkle improvement, elasticity enhancement, skin whitening, antioxidation, atopic improvement, skin moisturizing, skin texture improvement and skin barrier enhancement comprising the ferrybine of the present invention as an active ingredient can be used for the preparation of external preparation for skin .

When the peribaine is used as an active ingredient of the external preparation for skin, it may further contain at least one selected from the group consisting of a fatty substance, an organic solvent, a solubilizing agent, a thickening agent and a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizer, a foaming agent, A preservative, a vitamin, a blocking agent, a wetting agent, a essential oil, a dye, a pigment, a hydrophilic or lipophilic active agent, a lipid vesicle or a skin external preparation for oral administration in the form of an active agent, water, ionic or nonionic emulsifier, filler, sequestering agent and chelating agent And may contain adjuvants conventionally used in the field of dermatology, such as any other ingredient commonly used. The components can also be introduced in amounts commonly used in the field of dermatology.

When the peribaine is provided as an external preparation for skin, it may have a formulation such as, but not limited to, ointments, patches, gels, creams or sprays.

The compositions of the present invention may also be used for the manufacture of pharmaceutical formulations. Thus, the composition of the present invention may comprise a pharmaceutically acceptable salt of said ferbine. The pharmaceutically acceptable salt of the peribin may be an acid addition salt formed using an organic acid or an inorganic acid, and the organic acid may be, for example, formic acid, acetic acid, propionic acid, lactic acid, butyric acid, isobutyric acid, trifluoroacetic acid, , Malonic acid, malonic acid, fumaric acid, succinic acid, succinic acid monoamide, glutamic acid, tartaric acid, oxalic acid, citric acid, glycolic acid, glucuronic acid, ascorbic acid, benzoic acid, phthalic acid, salicylic acid, anthranilic acid, dichloroacetic acid, Sulfonic acid, p-toluenesulfonic acid and methanesulfonic acid-based salts, and the inorganic acid includes, for example, hydrochloric acid, bromic acid, sulfuric acid, phosphoric acid, nitric acid, carbonic acid and boric acid-based salts. Preferably in the hydrochloride or acetate form, more preferably in the hydrochloride form.

The above-mentioned acid addition salts may be prepared by a) directly mixing the feriin and the acid, or b) dissolving and mixing one of them in a solvent or a water solvent, or c) adding the feriin to the acid in a solvent or an anhydrous solvent ≪ / RTI > and mixing them.

In addition to the above, additionally saltable forms include, but are not limited to, the salts of gabapentin, pregabalin, nicotinate, adipate, hemimarate, cysteine, acetylcysteine, methionine, arginine, Aspartate and the like.

The pharmaceutical formulations comprising the composition may further comprise one or more active ingredients which exhibit the same or similar functions. For example, it may contain known skin regeneration, wrinkle improvement, elasticity enhancement, skin whitening, antioxidation, atopic improvement, skin moisturizing and skin tone improving ingredients. When the composition of the present invention contains the ingredient for improving skin regeneration, wrinkle improvement, elasticity enhancement, skin whitening, antioxidation, atopic improvement, skin moisturization and skin texture improvement, skin regeneration, wrinkle improvement, elasticity enhancement, skin whitening, antioxidation, atopy improvement , The skin moisturizing effect and the skin texture improving effect can be further enhanced. When the above ingredients are added, skin safety, easiness of formulation, and stability of effective ingredients can be considered according to the combined use. In one embodiment of the present invention, the composition is a skin regeneration component known in the art comprising retinoic acid, TGF, protein from animal placenta, betulinic acid and chlorella extract, antioxidant components known in the art, such as tocopherol, selenium , Vitamin C and phenolic compounds, whitening ingredients known in the art, substances inhibiting the activity of tyrosinase enzymes such as kojic acid and arbutin, hydroquinone, vitamin C (L- Ascorbic acid, derivatives thereof, and various plant extracts. The additional ingredients may be included in an amount of 0.0001 wt% to 10 wt% based on the total weight of the composition, and the content range may be adjusted according to requirements such as skin safety, easiness in formulating the peribin.

In addition, the composition of the present invention may further comprise a pharmaceutically acceptable carrier.

Pharmaceutically acceptable carriers may contain a variety of ingredients such as buffer, injectable sterile water, normal saline or phosphate buffered saline, sucrose, histidine, salts and polysorbates, and the like.

The composition of the present invention can be administered orally or parenterally, and can be administered in the form of a general pharmaceutical preparation, for example, various forms of oral and parenteral administration at the time of clinical administration. In the case of formulation, a filler, , A binder, a wetting agent, a disintegrant, a surfactant, and the like.

Solid formulations for oral administration include tablets, pills, powders, granules, capsules and the like, which may be prepared by mixing the pharmaceutical composition of the present invention with at least one excipient such as starch, calcium carbonate, Sucrose, lactose, gelatin, and the like.

In addition to simple excipients, lubricants such as magnesium stearate talc are also used. Examples of liquid formulations for oral administration include suspensions, solutions, emulsions, syrups and the like. Various excipients such as wetting agents, sweeteners, fragrances, preservatives and the like may be included in addition to water and liquid paraffin, which are commonly used simple diluents.

Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. Propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like can be used as the non-aqueous solvent and suspension agent. Examples of the suppository base include witepsol, macrogol, tween 61, cacao butter, laurin, glycerogelatin and the like.

When the composition of the present invention contains an effective amount of the above-mentioned peribaine, the skin regeneration effect, the wrinkle improvement effect, the elasticity enhancement effect, the skin whitening effect, the antioxidation effect, the atopy improvement effect, the moisturizing effect, Can be provided. In the present invention, the term "effective amount" refers to an amount effective for promoting regeneration of damaged skin, improving wrinkles, improving elasticity, exhibiting a whitening effect, inhibiting or alleviating oxidation of cells, Means the amount of a compound capable of improving dry skin such as atopy, improving moisturizing effect, improving skin barrier, or improving skin texture. The effective amount of the ferbine contained in the composition of the present invention will vary depending on the form in which the composition is commercialized, the method in which the compound is applied to the skin, and the time on the skin. For example, when the composition is commercialized in a pharmaceutical formulation, it may contain the feriBin at a higher concentration than when commercialized into cosmetics that are routinely applied to the skin. Accordingly, the daily dose is 0.1 to 100 mg / kg, preferably 30 to 80 mg / kg, more preferably 50 to 60 mg / kg, based on the amount of the ferbine, ≪ / RTI >

The composition of the present invention may be used alone or in combination with methods using surgery, radiation therapy, hormone therapy, chemotherapy, and biological response modifiers.

Further, the composition for skin regeneration, wrinkle improvement, elasticity enhancement, skin whitening, antioxidation, atopic improvement, skin moisturizing, skin texture improvement and skin barrier enhancement of the present invention can be used for manufacturing food formulations.

The food formulation, preferably a health food, means a food prepared by adding the fermented beverage to a food material such as a beverage, a tea, a spice, a gum, a confectionery, or the like, or encapsulating, pulverizing or suspending the fermented beverage. The food preparation, preferably a health food, is advantageous in terms of health, and unlike a pharmaceutical formulation, there is no side effect that may occur when a drug is taken for a long time using a food as a raw material.

Since the food preparation can be routinely ingested, it is very useful because it can expect high skin regeneration, wrinkle improvement, elasticity enhancement, skin whitening, antioxidation, atopic improvement, skin moisturizing, skin texture improvement and skin barrier enhancement.

When the ferbine is used as a food additive, the ferbine may be directly added or used together with other food or food ingredients, and may be suitably used according to a conventional method. The amount of the active ingredient to be mixed can be suitably determined according to its intended use (prevention, health or therapeutic treatment). Generally, the composition of the present invention is added in an amount of not more than 15 parts by weight, preferably not more than 10 parts by weight, based on the raw material, when the food or beverage is produced. However, in the case of long-term consumption intended for health and hygiene purposes or for health control purposes, the amount may be less than the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount exceeding the above range .

There is no particular limitation on the kind of the food. Examples of foods to which the above substances can be added include dairy products including meats, sausages, breads, chocolates, candies, snacks, confections, pizza, ramen noodles, gums, ice cream, soups, drinks, tea, Alcoholic beverages, and vitamin complexes, all of which include health foods in a conventional sense.

When the food is a beverage, various flavors or natural carbohydrates may be added as an additional ingredient such as a normal drink. The above-mentioned natural carbohydrates are sugar saccharides such as monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, polysaccharides such as dextrin and cyclodextrin, and xylitol, sorbitol and erythritol. Examples of sweeteners include natural sweeteners such as tau martin and stevia extract, synthetic sweeteners such as saccharin and aspartame, and the like. The ratio of the natural carbohydrate is generally about 0.01 to 0.04 g, preferably about 0.02 to 0.03 g per 100 mL of the composition of the present invention.

In addition to the above, the food formulations may contain various nutrients, vitamins, electrolytes, flavors, colorants, pectic acid and salts thereof, alginic acid and its salts, organic acids, protective colloid thickeners, pH adjusters, stabilizers, preservatives, glycerin, And the like. Other food formulations may contain flesh for the production of natural fruit juices, fruit juice drinks and vegetable drinks. These components may be used independently or in combination. The proportion of such additives is not critical, but is generally selected in the range of 0.01 to 0.1 parts by weight per 100 parts by weight of the composition of the present invention.

The present invention also provides a composition for stimulating stem cell activity comprising the above-mentioned fibrin as an active ingredient.

The term 'stem cell' in the present invention is a cell capable of cell division by itself and capable of differentiating into a very specific type of specific cell type. The type of such stem cells is not particularly limited, and in one embodiment, the stem cells may be dermal stem cells. The term 'dermal stem cells' refers to stem cells that can be differentiated into cells constituting the skin (epidermis, dermis and subcutaneous fat layer). The cells that make up the skin include keratinocytes, melanocytes, and fibroblasts (mainly responsible for biosynthesis of collagen and elastin) present in the epidermis.

The kind of the skin stem cell is not particularly limited. The dermal stem cells used in the present invention can be used irrespective of where they originate from. For example, dermal stem cells may be obtained from a known source of dermal stem cells, e. G., From the basal layer of hair follicles or epidermis, and the animal to be harvested may be a mammal. In one embodiment, the mammal may include, but is not limited to, a human, a mouse, a rat, a guinea pig, a rabbit, a monkey, a pig, a horse, a cattle, a sheep, Preferably the mammal can be human. Such methods of obtaining dermal stem cells from dermal stem cell sources are well known in the art.

In the present invention, 'stem cell activity promoting effect' refers to a stem cell proliferation promoting effect and / or a stem cell maintaining effect. The stem cell maintenance is effected by promoting expression of p63, which is a maintenance marker of dermal stem cells, as an intracellular transcription factor when treating ferbain into stem cells.

In addition, the composition for promoting stem cell activity of the present invention can be used for the production of cosmetic formulations. The skin stem cells play an important role in maintaining the health, physiological and biochemical homeostasis of the skin. When the skin stem cells become dysfunctional due to aging, various problems arise due to breakdown of the homeostasis of the skin. Therefore, by using the composition for promoting stem cell activity of the present invention as a cosmetic formulation, the activity of skin stem cells can be promoted, and skin regeneration, wrinkle improvement and elasticity enhancement effect can be exhibited. These cosmetic formulations are the same as those described in the description of cosmetic formulations including peribin.

Hereinafter, embodiments of the present invention will be described in detail to facilitate understanding of the present invention. However, the embodiments according to the present invention can be modified into various other forms, and the scope of the present invention should not be construed as being limited to the following embodiments. Embodiments of the invention are provided to more fully describe the present invention to those skilled in the art.

Reference Example  One: Perivine  Material Information

[Chemical Formula 1]

Figure pat00006

CAS No .: 2673-40-7

Where to buy: Sichuan Hongjie Import & Export CO., LTD

Reference Example  2: Serum-free Under medium conditions Of dermal stem cells  culture

Human epidermal stem cells purchased from Cellntec were added to 48-well plates (6 × 10 3 cells / well), supplemented with BPE (bovine pituitary extract) similar to fetal bovine serum And cultured for 24 hours at 5% CO 2 and 37 ° C using CNT-57 medium (Cellntec). Thereafter, the culture medium was removed through a suction tube, and the culture medium was removed using a PBS solution (GibcoBRL). Perivine (0.1 ㎍ / mL) was treated with CNT-57 medium containing no BPE, CO 2 and 37 ° C for 72 hours.

Experimental Example  One: CCK -8 Through evaluation method Dermal stem cells  Proliferation promoting effect

The skin stem cells cultured in Reference Example 2 were evaluated for CCK-8 (Cell counting kit-8). The CCK-8 evaluation is an indirect method of measuring the density of living cells by measuring the absorbance of Formazan, a dehydrogenase in the intracellular electron transport system, produced by decomposition of tetrazolium salt.

Cells were treated spectrophotometrically by treating CCK-8 solution (treated with 1/10 of the medium volume) at 37 ° C for 2 hours and then measuring the absorbance at 450 nm. The proliferation rate (%) of the skin stem cell of Perivin was calculated from the measured absorbance value in accordance with the following equation (1) with respect to the control group (CNT-57 medium supplemented with BPE), and the value thereof is shown in Table 1 below.

[Equation 1]

Growth rate (%) = (absorbance of sample-treated group / absorbance of control group) x 100

Promoting the proliferation of dermal stem cells Additive sample density Cell proliferation (%) versus control Ferry bin 0.1 / / mL 212.2% Control group - 100%

As shown in Table 1, the skin stem cells in the medium treated with feriBin showed excellent proliferation promoting effect.

Experimental Example  2: Of dermal stem cells  Stem cell ( stemness ) Confirm maintenance effect

The expression level of p63 was evaluated in order to confirm the stem cell maintenance effect of the dermal stem cells cultured in Reference Example 2 above. p63 is an intracellular transcription factor and is well known as a maintenance marker for dermal stem cells. Cells were isolated and cDNA was synthesized. Then, real-PCR was performed with Taqman staining solution to measure the expression level of p63. The concentration of RNA in this experiment was normalized to S16 ribosomal RNA.

The experimental results are shown in Table 2 below. In Table 2 below, the numerical value of the increase in p63 is a multiple of the control group (CNT-57 medium supplemented with BPE).

Stem cell (stem cell) maintenance effect (number of repeats = 3) Additive sample density p63 Incremental multiple (times) Ferry bin 0.1 / / mL 1.59 Control group - 1.0

As shown in Table 2, the medium condition treated with peribin promotes the expression of p63 in dermal stem cells, and thus has excellent stem cell maintenance effect.

Example  1: Promoting collagen synthesis

Peribin was added to the culture medium of human - derived fibroblasts to examine the effect of promoting collagen synthesis at the cellular level. The biocompatible collagen was quantitated using a PICP EIA kit (Procollagen Type I C-Peptide Enzyme Immunoassay Kit).

Perivene was added to the culture medium of human fibroblasts (7 × 10 4 cells / cm 2 ) together with vitamin C and a control (no supplement) to a final concentration of 0.05 μg / mL and 0.1 μg / After culturing, the culture was taken and the degree of collagen biosynthesis at each concentration was measured at 450 nm using a spectrophotometer with a PICP EIA Kit. The collagen biosynthesis performance was calculated by the relative performance relative to the control group and the results are summarized in Table 3 below.

Promotion of collagen synthesis by concentration (number of repeats = 3) Additive sample Application concentration (㎍ / mL) Increase in collagen synthesis (%) compared to control Ferry bin 0.05 / / mL 35.2% Ferry bin 0.1 / / mL 48.3% Vitamin C 50 / / mL 30.6%

As shown in Table 3, peribin has excellent collagen synthesis ability against human-derived fibroblasts, and it is possible to obtain more excellent collagen synthesis effect at a lower concentration than in the case of using vitamin C, which is generally known to have a capability of collagen synthesis .

Example 2: Whitening effect - Confirmation of melanin formation inhibitory effect

Perivene was added to the culture medium of rats' B-16 mouse melanoma cells to examine the whitening effect at the cellular level (Lotan R., Lotan D. Cancer Res. 40: 3345-3350, 1980). At this time, the toxicity of the melanoma cells of the rats before the experiment was evaluated, and the whitening evaluation was performed by selecting the concentration without toxicity.

Perivin was added to the culture medium to a final concentration of 0.05 μg / mL and 0.1 μg / mL, and the control group, arbutin, was added to the medium to a concentration of 200 μg / mL, and treated with B-16 melanoma cells for 3 days Respectively.

Cells were then trypsinized, detached from the culture, centrifuged, and extracted with melanin. The removed cells were incubated with 1 mL of sodium hydroxide solution (1N), boiled for 10 minutes to dissolve melanin, and the absorbance was measured at 400 nm using a spectrophotometer to measure the amount of melanin produced.

The amount of melanin was measured by an absorbance of 10 6 cells per unit cell, and the amount of melanin produced relative to the control group was calculated as inhibition (%). The results are summarized in Table 4 below.

Inhibitory effect of melanin formation at the cellular level (number of repeats = 3) sample Inhibition rate (%) Control group (no addition) - Control group 1: Arbutin (200 쨉 g / mL) 30.8 Perivene (0.1 [mu] g / mL) 40.2 Perivene (0.05 / / mL) 25.6

As shown in Table 4, it can be seen that peribin has remarkably superior melanin production inhibitory effect on melanoma cells of the rat cultured as compared with albutin, which is a known whitening substance.

Example  3: Antioxidant effect - Free radical scavenging rate

Free radical scavenging activity was measured to confirm antioxidative activity of feriBin. Free radical scavenging activity was measured using DPPH. DPPH was purchased from Sigma Co. (Sigma Co., Ltd, USA) and used. First, a standard DPPH ethanol solution of 1.5 mM (0.06 mg / mL) was prepared. Then, ethanol was added to peroxide and ascorbic acid, which is an antioxidant, as a reference material, respectively, to prepare samples at concentrations of 50 ㎍ / mL, 25 ㎍ / mL, 12.5 ㎍ / mL, 6.25 ㎍ / mL and 3.125 ㎍ / mL. Then, the sample and standard DPPH solution were added at the same ratio, stirred well, reacted at 37 ° C for 30 minutes, and absorbance was measured at 520 nm. At this time, ethanol was added instead of the sample to give a control group. Free radical scavenging activity and the results are obtained for the IC 50 half maximal inhibitory concentration (median inhibition) shown in Table 5. IC 50 is a common method of expressing free radical scavenging activity as a concentration of ascorbic acid and ferbine required to remove 50% of the free radicals of the no-added control group.

Free radical scavenging rate (IC 50 ) sample Free radical scavenging rate (占 퐂 / mL) Ascorbic acid 5.2 Ferry bin 3.9

As shown in Table 5, ferbine exhibits antioxidative effects because it shows high activity as compared with ascorbic acid, which is a known antioxidant.

Example  4: PPAR  Verification of Alpha Activation Promotion Effect

In order to confirm the PPAR alpha activation promoting effect of peribin, the following experiment was performed using peribin. C2C12 cells (ATCC CRL-1772), a mouse myoblast cell line, were subcultured in DMEM (Dulbecco's Modified Eagle's Medium) medium containing 10% fetal bovine serum. As the vector used herein,

1) A DNA fragment encoding GAL4-DBD (DNA Binding Domain), which is a transcription factor of yeast, and Ser 167 to Tyr 468 in human PPAR alpha LBD (Ligand Binding Domain), into a SV40 promoter of pZEO vector (Invitrogen) NM 005036);

2) a GAL4 response sequence inserted into a multiple restriction enzyme recognition site of a pGL3 vector (Promega) expressing luciferase and 3) a vector inserted into a transfection internal control with? -Galactosidase β-galactodisase) was used.

The cultured cells were plated on a 24-well plate at a concentration of 4 × 10 4 and cultured for 12 hours. The three types of plasmid genes were transiently transfected using lipopectamine . After 8 hours, the compound of formula (1) was treated and incubated for 12 hours, washed with 1 x PBS, lysed with 1 x reporter lysis buffer, and luciferase assay kit (Promega) and The activity of luciferase was measured using? -galactosidase assay kit (Promega). That is, the PPAR alpha activity was measured as " luciferase activity / beta -galactose activity ".

Wy-14,643 (Calbiochem), the most potent ligand of PPAR alpha, was used as a positive control in this experiment, and DMSO 0.05% was used as a negative control.

According to the above experimental method, the activity of PPAR alpha was measured according to the concentration by treating the ferbain with C2C12 cells. The results are shown in Table 6 below. The values of PPAR alpha activity in Table 6 below are percentages relative to the negative control (0.05% DMSO).

Activity of PPAR alpha sample PPAR alpha activity (%) DMSO (0.05%) 100 Wy-14,643 (0.5 [mu] M) 280 Wy-14,643 (1 [mu] M) 935 Peribin (0.01%) 583 Peribin (0.1%) 972

As shown in Table 6, compared with the positive control, it was confirmed that feriBin exhibited a very strong PPAR alpha activity.

Example  5: Filaggrin's  Promoting effect of expression

Perivene was added to the culture medium of human keratinocyte HaCaT cells at a concentration of 0.05 μg / mL and 0.1 μg / mL for 1 day. RNA was isolated from the cells, cDNA was synthesized, and Real- time PCR. The RNA concentration was normalized to S16 ribosomal RNA.

Effect of expression of pillar green depending on concentration (number of repeats = 3) Additive sample Increase in pillar green compared to control Control group (no addition) 1.00 Perivene (0.05 / / mL) 15.0 Perivene (0.1 [mu] g / mL) 24.4

As shown in Table 7, ferbine has the ability to promote pilar green expression of human keratinocyte HaCaT cells.

Example  6: To improve the turn-over of the skin  About Efficacy

About the nutritional cream of the following formulation example 2, the effect of the improvement of the exfoliation of the skin in the healthy twenties to 50 women was tested as follows.

Twenty of 20 women aged 20 to 50 years were given a 1.5% solution of DHA (Dihydroxyacetone, Sigma Aldrich, USA) for 8 hours, followed by deposition. The preparation cream was applied twice daily to the test site. After 4 days of application, 8 days after application, 12 days after application, photographs were taken using instrument Chromameter CR-400 (Minolta, Japan) and skin lightness of DSLR and DSLR. The measured values were evaluated by three mean values excluding the maximum value and the minimum value. The higher the improvement in the skin brightness of the deposition site, the better the improvement of the exfoliation. The results are shown in Table 8 below.

Skin brightness improvement effect division Four days after application Eight days after application After 12 days of application Improvement rate (%) 1.12 3.98 10.47

As shown in Table 8, when the nutritional cream according to the present invention was used, it was found to have the effect of improving the exfoliation of the skin.

Formulation example  1: Preparation of pharmaceutical preparations

1. Preparation of tablets

Ferribine 0.2 mg

100 mg of corn starch

100 mg of milk

2 mg of magnesium stearate

After mixing the above components, tablets were prepared by tableting according to a conventional method for producing tablets.

Formulation example  2: Manufacture of cosmetics

1. Manufacture of nutritional cream

As in the following composition, a nutrition cream containing peribaine as an active ingredient was prepared according to a conventional method.

Peribin 0.2 wt%

Beta-1,3-glucan 5.0 wt%

Wax 10.0 wt%

Polysorbate 60 1.5 wt%

≪ tb > < tb > < tb >

0.5% by weight of sorbitan sesquioleate

Liquid paraffin 10.0 wt%

Squalane 5.0 wt%

Caprylic / capric triglyceride 5.0 wt%

Glycerin 5.0 wt%

3.0% by weight of butylene glycol

3.0% by weight of propylene glycol

0.2% by weight triethanolamine

Preservative 0.05 wt%

0.05% by weight of pigment

0.05% by weight fragrance

Purified water to 100%

Formulation example  3: Preparation of external preparation for skin

1. Manufacture of ointment

As in the following composition, an ointment containing peribaine as an active ingredient was prepared by a conventional method.

Feribene 0.5 wt%

Beta-1,3-glucan 10.0 wt%

Wax 10.0 wt%

Polysorbate 60 5.0 wt%

≪ tb > < tb > < tb >

0.5% by weight of sorbitan sesquioleate

Vaseline 5.0 wt%

Liquid paraffin 10.0 wt%

Squalane 5.0 wt%

SHARE BUTTER 3.0 wt%

Caprylic / capric triglyceride 5.0 wt%

Glycerin 10.0 wt%

Propylene glycol 10.2 wt%

0.2% by weight triethanolamine

Preservative 0.05 wt%

0.05% by weight of pigment

0.05% by weight fragrance

Purified water to 100%

Claims (12)

A composition for skin regeneration comprising perivine as an active ingredient. A composition for improving skin wrinkles or elasticity, comprising peribene as an active ingredient. A composition for skin whitening comprising feribene as an active ingredient. An antioxidative composition comprising peribene as an active ingredient. A composition for improving atopy comprising feribene as an active ingredient. A composition for moisturizing skin comprising peribene as an active ingredient. A composition for improving skin texture comprising peribene as an active ingredient. A composition for stimulating stem cell activity comprising ferbain as an active ingredient. 9. The composition according to claim 8, wherein the composition promotes stem cell proliferation or maintains stem cells. [Claim 11] The composition according to claim 9, wherein the composition promotes the expression of p63 gene to maintain stem cell characteristics. A composition for strengthening skin barrier comprising peribin as an active ingredient. The composition according to any one of claims 1 to 8 and 11, wherein the composition is for the manufacture of pharmaceutical, cosmetic, food or dermatological formulation.
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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH08208424A (en) 1994-12-20 1996-08-13 Unilever Nv Cosmetic composition containing betulinic acid
JPH08231370A (en) 1995-02-23 1996-09-10 Taiyo Kagaku Co Ltd Skin cosmetic
JPH0940523A (en) 1995-07-28 1997-02-10 Ichimaru Pharcos Co Ltd Fibroblast proliferation promoter containing water extract form chlorella
JPH1036283A (en) 1996-07-18 1998-02-10 Ichimaru Pharcos Co Ltd Fibroblast proliferation promoting agent

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH08208424A (en) 1994-12-20 1996-08-13 Unilever Nv Cosmetic composition containing betulinic acid
JPH08231370A (en) 1995-02-23 1996-09-10 Taiyo Kagaku Co Ltd Skin cosmetic
JPH0940523A (en) 1995-07-28 1997-02-10 Ichimaru Pharcos Co Ltd Fibroblast proliferation promoter containing water extract form chlorella
JPH1036283A (en) 1996-07-18 1998-02-10 Ichimaru Pharcos Co Ltd Fibroblast proliferation promoting agent

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
1. Cardinale G. et al, Adv. Enzymol., 41, p. 425, 1974
2. Feingold 외, J. Invest. Dermatol., 110, pp 368-375, 1998.
3. Feingold 외, J. Invest. Dermatol., 115, pp 353-360, 2000.
4. Journal of the European Academy of Dermatology and Venereology. 12:103-114, 1999
5. The British journal of dermatology. 110: 129-138, 1984, Skin research and technology.5:189-194, 1999
6. Epidermal Stem Cells of the Skin, Cedric Blanpain, Elaine Fuchs, Annual Review of Cell and Developmental Biology, November 2006, Vol. 22, Pages 339-373
7. Human skin stem cells and the ageing process, Catherin Niemann, Stem Cell Aging and Regenerative Medicine, November 2008, Pages 986-997

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