JPH1036279A - Fibroblast proliferation promoting agent containing vegetable extract - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain the subject agent effective for the prevention and amelioration of various diseases of the skin and/or scalp and giving a preparation for external use for skin, a bathing agent, etc., having high safety by using a plant extract having fibroblast proliferation promoting action. SOLUTION: This agent contains an extract produced by extracting one or more kinds of plants selected from almond, common dandelion, elder tree, Cnidiumofficinale, Swerrtia japonica, Sohakuhi, Tounin, Panax ginseng, hop, althea and Coix laryma-jobi with water, (anhydrous) ethanol, 1,3-butylene glycol, propylene glycol or hexylene glycol or a mixture of two or more of the above solvents. The amount of the agent is >=0.01wt.%, preferably 0.1-60wt.% in the case of compounding the agent to a preparation for external use for skin, a bathing agent or foods and beverages. Diseases such as eczema, chapped skin, dry skin, etc., can safely and effectively be prevented and ameliorated by the use of the agent.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

The present invention relates to a novel and safe plant extract containing at least one plant extract selected from almonds, dandelion, sambucus nigra, senkyu, assembly, Souakuhaku, tounin, carrot, hop, mugwort, and yokunin. Fibroblast proliferation promoter, and various skin external preparations in the field of drugs, quasi-drugs or cosmetics.
The present invention relates to bath agents and applications to foods and drinks.

[0002] The field of application is generally applicable to various internal and external preparations (including preparations used for animals), and specifically, ampules, capsules, pills, tablets, and powders. 1) Pharmaceuticals, 2) Quasi-drugs, 3) Foods, 4) Topical or systemic skin cosmetics, 5) Scalp / hair Pharmaceutical and / or cosmetic preparations (eg, shampoos, rinses, treatments, permanent solutions, hair dyes,
Hairdressing agents, hair tonics, hair growth / hair restoration, etc.), 6) bath agents for use in bath water, 7) other liquid odor / odor control agents, sanitary articles, sanitary cotton, wet tissues, etc. .

In addition, it is used for various injuries such as skin and scalp, oral cavity, eyes, ears, nose, anus, and genital area for the purpose of promoting the growth of skin and dermis, preventing skin aging, roughening the skin and promoting wound healing. be able to.

[0004] Foods and drinks further include oral compositions (gum, candy, etc.), processed seafood products such as kamaboko and chikuwa, livestock products such as sausage and ham, western confectionery, Japanese confectionery, raw noodles, Chinese noodles, and yuden noodles , Buckwheat and other noodles, sauces, soy sauce, sauce, sugar, honey, powdered candy, water syrup, and other spices, curry powder, mustard powder, pepper powder and other spices, jam, marmalade, chocolate spread, pickles, soy sauce, Processed vegetables and fruits such as sprinkles and canned and bottled vegetables and fruits, dairy products such as cheese, butter, yogurt, miso soup, soup, fruit juice, vegetable juice, whey drinks, soft drinks, alcoholic beverages, etc. Use in general foods and drinks such as health foods.

[0005]

2. Description of the Related Art Individual aging or various diseases caused by the aging have a correlation with aging of all cells that can divide (reduction of division rate and cell function). As a result, a large number of cell activators have been searched for. Among them, one of the cell activators is cell growth factor, and various factors have already been confirmed, and the applicant's connective tissue hydrolyzate (Japanese Patent Application Laid-Open No. 62-84024), and water solubility derived from organs such as thoracic line and spleen Protein (Japanese Unexamined Patent Publication No. 63-188697,63
-188698), or bovine placenta extract (JP-A-03-141299) or an extract of bovine brain, bovine pituitary gland, and cockscomb (JP-A-01-175)
998), which are used for quasi-drugs and cosmetics.

[0006]

However, these are all derived from animal systems. As plant-based ones, wolfberry is a cell activator (such as a cell activator, which promotes cell engraftment rate and colony formation). Japanese Patent Application Laid-Open No. 02-117686), but at present, no plant-derived plant having cell growth ability has been obtained.

[0007] In view of these circumstances, the present inventors have developed various plants or ingredients which are useful as fibroblast growth promoters, and as a result, as a result, almonds, dandelions, western elderberry, senkyu, assembly,
It has been confirmed that the extracts of Sohakuhi, Tonin, Carrot, Hops, Mukuge, and Yokuinin have a fibroblast proliferation promoting effect, and further prevent and improve various diseases of the skin / scalp (eg, eczema, rough skin, skin swelling, etc.). The present invention has been completed by providing a skin external preparation, a bath preparation, and a food or drink which are effective and highly safe.

[0008]

[Means for Solving the Problems]

BEST MODE FOR CARRYING OUT THE INVENTION The term "almond: peach" used in the present invention means "Rosaceae" and "Prunus" (Pr.).
unus) Plant almond (Prunus amygdalus Batsch)
Seeds.

[0009] The "dandelion" is obtained from the roots or whole plants of the plant Dandelion (Taraxacum officnale Weber) of the genus Taraxacum (Compositae). Taraxacum albidum Dahlst., Perilla dandelion (Taraxacum japonicum Koidz.), Moko dandelion (Taraxacum monogolicum Hand.-Mazz), keirin dandelion (Taraxacum monogolicum Hand.-Mazz. Var.
corniculatum Nakai, Taraxacum asiaticum Dahls.),
European dandelion (Taraxacum platycarpum Dahls)
t.), dandelion (Taraxacum alpicola Kita)
m.), red dandelion (Taraxacum laevigatum DC.),
Ezo Dandelion (Taraxacum venustum Koidz.), Chinese dandelion "Taraxacum sinicum Kitag. (= Taraxacum sinic
um Dahlst.), Taraxacum helerolepis Nakai etH.Koid
z., ", Odasum Dandelion (Taraxac)
um platycarpum Diels, Taraxacum erythrodium Kita
g., et al., Taraxacum ceratophorum DC., ", Manxius dandelion" Taraxacum pseudo-albidum Kitag., Tarax
acum pseudo-albidum Kitag.var.lutescens Kitag., Ta
raxacum ohwianum Kitam., Taraxacumdisrectum Lede
b., ”, a Japanese beetle (Taraxacum cuspidatum Dahls
t.), etc., can also be used.

[0010] "Sambucus nigra" refers to those obtained from the fruit, flower or foliage of the plant Sambucus nigra L. of the genus Sambucus (Caprifoliaceae). Elderberry `` Sambucus racemosaL.subsp.sieboldiana (Mi
q.) Hara (= Sambucus sieboldiana (Miq.) Blume ex Gra
ebn.), Sambucus sieboldiana (Miq.) B
lume ex Graebn.var.miquelii (Nakai) Hara), American elderberry (Sambucus candensis L.), and elderberry (Sambucus javanica Reinw.ex Bl.subsp.chinensi
s), fruit / flower or foliage of Sambucus williamsii Hanse.

[0011] "Sengyu: river kyuu" is a rhizome of a plant mushroom (Cnidium officinale Makino) belonging to the genus Umbelliferae (Cnidium).

The term “assembly: this drug” refers to the Gentianaceae (Ge
ntianaceae) Plant assembly of the genus Ophelia "O
phelia japonica (Schult.) Griseb. (= Swertia japoni
ca (Schult.) Makino) ”.

"Sohakuhaku: mulberry bark" refers to the mulberry family (Mo
raceae) Mulberry (Morus) plant mulberry (Morus bombycis)
Koidzumi), magwa (Morus alba L.) or other root plants of the same genus are used. For example, Shimaguwa (Morus austra
lis Poir.), Logwa (Moruslatifolia (Bur.) Poir.),
Moruko monkey (Morus mongolica (Bur.) Schneid.), Kuromigwa (Morus nigra L.) Akamigawa (Morus rubra)
L.).

"Tounin: peach" refers to Rosaceae (Rosaceae).
ae) Prunus peach (Prunus persica B)
atsch) or peach (Prunus persica Batsch var.david)
ianaMaximowicz) Santo “Prunus davidiana (Ca
rr.) Seeds of Franch. "

"Carrot: ginseng" refers to a carrot (Aral)
iaceae) genus Panax ginseng (Pana ginseng)
x) Plant Panax ginseng CAMeyer
(= Panaxschinseng Nees) "or steamed and dried roots.

"Hop" is a plant hop (Humulus) of the genus Moraceae (Humulus).
lupulus Linne).

"Mukuge: Ki-geun" refers to Malvaceae (Malvac)
eae), the astragalus of Hibiscus plant: Tree Geun (Hib
Use those obtained from the flowers, fruits, bark, leaves, and roots of isucus syriacus L., but also other species of the same species, Hibisucus rosa-sinensis L., Furinbussouge (Hibisucus schizopetalus Hook.f.), and Roselle (Hibis)
ucus sabdariffa L.), (Hibisucus arnottianus A. Gr
ay), (Hibiscus kokio Hillebr.), and the like, flowers, fruits, bark, leaves, and roots of plants commonly called hibiscus can also be used.

"Yokinin" is a graminea (Graminea)
e) The coix plant of the genus Coix (Coix lach)
ryma-jobi L.var.ma-yuen (Roman) Stapf (= Coix ma-yuen
Roman) "seeds.

It should be noted that the almond, dandelion, elderberry, senkyu, assembly, soybean sprouts, tannin, carrot, hops, mugwort and yokunin used in the present invention have no fibroblast proliferation promoting effect. unknown.

[0020]

BEST MODE FOR CARRYING OUT THE INVENTION The extracts of almonds, dandelion, elderberry, scutellaria japonica, scutellaria, radish, carrot, carrot, hops, mugwort and yokunin used in the present invention are defined as the various parts of each plant (whole plant). , Seeds, fruits, ears, gland bodies, root bark, rhizomes, roots, bark, foliage, leaves, branches, flowers, etc.) as they are, or after drying or grinding, and extracted with a solvent.

As the extraction solvent, any one selected from water, ethanol, anhydrous ethanol, 1,3-butylene glycol, propylene glycol, and hexylene glycol, or a combination of two or more thereof may be used. Yes, each water, ethanol, absolute ethanol, 1,3-butylene glycol, propylene glycol,
Hexylene glycol extraction can also be used in combination.

The obtained extract may be optionally dried, concentrated or diluted depending on the type and form of the applied fibroblast growth promoter, skin external preparation and bath preparation, and food and drink. good.

The production method is not particularly limited, but it may be within the range of the boiling point of the solvent at normal temperature to normal pressure. After extraction, the solvent is adsorbed and filtered using an ion exchange resin.
A solution, paste, gel, or powder may be obtained by decoloring and purifying. In many cases, it can be used as it is, but if necessary, further purification treatment such as deodorization and decolorization may be added as long as the effect is not affected. An activated carbon column or the like may be used, and ordinary means generally applied to the extracted substance may be arbitrarily selected.

The almonds, dandelions of the present invention,
Sambucus elder, Senkyu, Assemble, Sawdust, Tonin, carrot, hop, mugwort, and yokunin extract can be directly used as a fibroblast growth promoter, and can be further incorporated into skin external preparations and bath preparations, foods and drinks. The amount is not particularly limited, but is slightly different depending on the type of fibroblast proliferation promoter, external preparation for skin and bath, type of food and drink, quality, expected effect, but usually 0.01 weight. % Or more (hereinafter represented by weight%), preferably 0.1 to 60%. If the amount is less than 0.01%, the effect cannot be expected sufficiently.

In addition, the fibroblast proliferation promoter, skin external preparation, bath preparation, food and drink of the present invention, if necessary, in addition to the above-mentioned essential components, may contain pharmaceuticals, as long as the effects of the present invention are not impaired. It can be produced by arbitrarily selecting and using components and additives used in pharmaceuticals such as quasi-drugs, cosmetics, foods and beverages, and the like.

(1) Various fats and oils Avocado oil, almond oil, fennel oil, perilla oil,
Olive oil, orange oil, orange luffer oil, sesame oil,
Cocoa butter, chamomile oil, carrot oil, cucumber oil, tallow, tallow fatty acid, kukuinut oil, safflower oil, shea butter, soybean oil, camellia oil, corn oil, rapeseed oil, persic oil, castor oil, cottonseed oil, peanut oil, Turtle oil, mink oil, egg yolk oil, cocoa butter, palm oil, palm kernel oil, mocro, coconut oil, tallow, lard, or hydrogenated products of these oils (hardened oil, etc.).

(2) Waxes Beeswax, carnauba wax, whale wax, lanolin, liquid lanolin, reduced lanolin, hard lanolin, candelilla wax,
Montan wax, shellac wax, etc.

(3) Mineral oil Liquid paraffin, vaseline, paraffin, ozokeride, ceresin, microcrystalline wax, squalene, squalane, pristane and the like.

(4) Fatty acids Natural fatty acids such as lauric acid, myristic acid, palmitic acid, stearic acid, behenic acid, oleic acid, 12-hydroxystearic acid, undecylenic acid, tall oil, lanolin fatty acid, isononanoic acid, caproic acid , 2-ethylbutanoic acid, isopentanoic acid, 2-methylpentanoic acid, 2
-Synthetic fatty acids such as ethylhexanoic acid and isopentanoic acid.

(5) alcohols ethanol, isopiranol, lauryl alcohol,
Natural alcohols such as cetanol, stearyl alcohol, oleyl alcohol, lanolin alcohol, cholesterol, and phytosterol; and synthetic alcohols such as 2-hexyldecanol, isostearyl alcohol, and 2-octyldodecanol.

(6) Polyhydric alcohols Ethylene oxide, ethylene glycol, diethylene glycol, triethylene glycol, ethylene glycol monoethyl ether, ethylene glycol monobutyl ether, diethylene glycol monomethyl ether, diethylene glycol monoethyl ether, polyethylene glycol, propylene oxide, propylene glycol , Polypropylene glycol, 1,3-butylene glycol, glycerin, pentaerythritol, sorbitol, mannitol and the like.

(7) Esters Isopropyl myristate, isopropyl palmitate, butyl stearate, hexyl laurate, myristyl myristate, oleyl oleate, decyl oleate, octyl dodecyl myristate, hexyl decyl dimethyloctanoate, cetyl lactate, Myristyl lactate, diethyl phthalate, dibutyl phthalate, lanolin acetate, ethylene glycol monostearate, propylene glycol monostearate, propylene glycol dioleate, and the like.

(8) Metal soaps Aluminum stearate, magnesium stearate, zinc stearate, calcium stearate, zinc palmitate, magnesium myristate, zinc laurate, zinc undecylenate and the like.

(9) Gum, saccharides and water-soluble polymer compounds Arabic gum, benzoin gum, dammar gum, guaiac, Irish moss, karaya gum, tragacanth gum,
Carob gum, quinseed, agar, casein, lactose,
Fructose, sucrose and its esters, trehalose and its derivatives, dextrin, gelatin, pectin, starch, carrageenan, carboxymethyl chitin or hydroxyalkyl (C ₂ -C ₄ ) oxide to which alkylene (C ₂ -C ₄ ) oxide such as ethylene oxide is added _{2 to} C ₄ ) chitin or chitosan, low molecular weight chitin or chitosan, chitosan salt, sulfated chitin or chitosan, phosphorylated chitin or chitosan, alginic acid and its salt, hyaluronic acid and its salt, chondroitin sulfate and its salt, heparin, ethyl cellulose , Methylcellulose, carboxymethylcellulose, carboxyethylcellulose, sodium carboxyethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose, nitrocellulose, crystalline cellulose Polyvinyl alcohol, polyvinyl methyl ether, polyvinyl pyrrolidone, polyvinyl methacrylate, polyacrylic acid salts, polyalkylene oxide or crosslinked polymer thereof such as polyethylene oxide or polypropylene oxide, carboxyvinyl polymers, such as polyethyleneimine.

(10) Surfactant Anionic surfactant (alkyl carboxylate, alkyl sulfonate, alkyl sulfate, alkyl phosphate), cationic surfactant (alkyl amine, alkyl quaternary ammonium salt) ), Amphoteric surfactant:
Carboxylic acid type amphoteric surfactant (amino type, betaine type), sulfate ester type amphoteric surfactant, sulfonic acid type amphoteric surfactant, phosphate type amphoteric surfactant, nonionic surfactant (ether type nonionic) Surfactants, ether ester type nonionic surfactants, ester type nonionic surfactants, block polymer type nonionic surfactants, nitrogen-containing nonionic surfactants), other surfactants (natural surfactants) , Derivatives of protein hydrolysates,
Polymer surfactant, surfactant containing titanium and silicon,
Fluorocarbon surfactants).

(11) Various vitamins Vitamin A group: retinol, retinal (vitamin A ₁ ), dehydroretinal (vitamin A ₂ ), carotene, lycopene (provitamin A), vitamin B group: thiamine hydrochloride, thiamine sulfate (Vitamin B ₁ ), riboflavin (vitamin B ₂ ), pyridoxine (vitamin B ₆ ), cyanocobalamin (vitamin B ₁₂ ), folic acids,
Nicotinic acids, pantothenic acids, biotins, choline,
Inositols, vitamin C group: ascorbic acid and its derivatives, vitamin D group: ergocalciferol (vitamin D ₂ ), cholecalciferol (vitamin D ₃ ),
Dihydrotaxerol, vitamin E group: tocopherol and its derivatives, ubiquinones, vitamin K group: phytonadione (vitamin K ₁ ), menaquinone (vitamin K ₂ ), menadione (vitamin K ₃ ), menadiol (vitamin K ₄ ), etc. Essential fatty acids (vitamin F), carnitine, ferulic acid, γ-oryzanol, orotic acid, vitamin Ps (rutin, eriocitrin, hesperidin), vitamin U and the like.

(12) Various amino acids Valine, leucine, isoleucine, threonine, methionine, phenylalanine, tryptophan, lysine, glycine, alanine, asparagine, glutamine, serine, cysteine, cystine, tyrosine, proline, hydroxyproline, aspartic acid, glutamic acid , Hydroxylysine, arginine, ornithine, histidine, and the like, and their sulfates, phosphates, nitrates, citrates, and amino acid derivatives such as pyrrolidone carboxylic acid.

(13) Various additives derived from plant or animal raw materials These are usually processed according to the type and form of the product to be added (for example, grinding, milling, washing, hydrolysis, hydrolysis, etc.). Fermentation, purification, squeezing, extraction, fractionation, filtration, drying, pulverization, granulation, dissolution, sterilization, pH adjustment, deodorization, decolorization, etc. You can choose to serve.

The solvent to be used for the extraction may be selected in consideration of the purpose and type of the product to be provided, or the processing to be performed later, but usually, water and a water-soluble organic solvent (for example, ethanol) , Propylene glycol, 1,3-butylene glycol, etc.).
However, when the content of the organic solvent is not preferable depending on the application, only water may be used, or ethanol which is easily removed after the extraction may be used, and may be used alone or in an arbitrary mixed solution with water. Exploited extract may be used.

In addition, additives derived from plant or animal raw materials are
When applied to systemic or topical external preparations and cosmetics, including protection of skin and hair, moisturizing, improvement of feel and texture,
Gives flexibility, relieves irritation, relieves stress due to aroma, activates cells (prevents cell aging), suppresses inflammation, improves skin and hair quality, prevents and improves rough skin, hair growth, hair growth, hair loss prevention, gloss Application, cleaning effect, alleviation of fatigue, promotion of blood flow,
In addition to cosmetic effects such as a warm bath effect, effects such as flavoring, deodorizing, thickening, preserving, and buffering can be expected.

In the case of edible products, health and cosmetic effects such as nutritional supplementation, recovery from fatigue, tonicity, cell activation (prevention of cell aging) and the like, improvement of taste, imparting of color tone, aroma and gloss, stabilization, It can be used for thickening and preservation. Furthermore, in addition to this, various cosmetic and pharmaceutical effects of each known raw material are expected, and by combining them, the intended effect is enhanced or multi-functional. It is also possible to make a product that expects an effect.

As a specific plant (crude) used as a raw material,
For example, Ai (Aiba), Aokazura (Seifu Fuji), Aotsuji Laji (Kibo), Acacia, Akasugi fruit, Akagrape, Akamegashiwa (Akabu Kashiwa), Akane (Akane grass root), Akajioji-jiou (Jihuang), Agi (Awei) , Akinire (royu skin),
Akebi (Kidori), Asa (Asako Jin), Malva Asagao or Asagao (Toshibushi), Ashitaba (Asuka), Azuki (Red Azuki), Acerola, Asenyaku (Asenyaku), Anise, Abemaki Fruit,
Avocado, Ama, Amacha (sweet tea), Amachaworu, Amadokoro (Tamatake), Amigasa lily (Shellfish), Altea, Arnica, Aloe (lux), Aloe vera, Angelica, Anzu Honganzu (Anjin), Ansokkou (Azusa), Igakozolina (Bile head), knotweed (tiger root), strawberry, fig
(Fructose) or its leaves, Ichibai (Aoiko), Ichiyakuso,
Ginkgo (Ginkgo biloba, Ginkgo biloba), Itohimemegi (Enshi), Carob, Rice seed or seed coat, Inondo seed, Ibuki-kusou-so, Nettle, Ylang-ylang, Fennel,
Ukiyagara (Mitsuryo), Uguisukagura fruit, Himeuikyo, Turmeric (Utsukin), Usubasaishin Keirinsaishin
(Spicy), Usbania oyster, Usbenitachioi, Utsubogusa (summer hay), Udo or Shishido (Qang, independent, Tang alone), Ume (Ume), or its flesh, Vladigashi, Uwaurushi, Unshu mandarin (Chin skin), Estragon, Eleuthero (Ezo Goka), Enoki, Ebisugusa (Keiko), Elderberry fruit, Elemi, Enju (Gossip, Gossip rice), Ogi / Kibanaogi (Associated Radish), Euren (Yellow), Ookarasuri (Karonin), Oooo Tsuzuraji (self-defense), psyllium (vegetative child, vegetative grass), toucan hazel (haruko), Obananaokera okera (white jujutsu), Obayashishabushi fruit, O hawthorn hawthorn (Yamako), barley (barley), oguruma (turning over), Okra fruit, St. John's wort, St. John's wort
Onigurumi, Oninoyagara (Tenma), Onyuri Sasasayuri Hakatayuri (Yuri), Ononis, Halibut (Hibaha Yu), Ominaeshi (Loose), Dutch Strawberry, Dutch Karash (Cresson), Dutch Jelly, Dutch Mitsuba, Olive,
Oregano, orange fruit or pericarp, calyxio (ripe yellow), cacao seeds, oysters or their leaves (persimmon), kagi kazura (chou chou), cassia, cassia ki fruit (kozomi), gajutsu
(Shazajutsu), Kashiwa (Kaju, Shaba), Cascalila, Kanixa
(Kin-sato), Valeriana officinalis (Kikose root), Birch or weeping birch (Birch birch), Pumpkin, Capoknoki seed, Kahokoku-sansho (Shupper), Gama (Kaba yellow), Chamomile-Roman chamomile, Kamiyatsude (Tsou-cho), Karasuri ), Rasabishaw (mid-summer), oats, guarana seeds, karaya, karin
(Gourd), Kawayanagi, Kawatake mushroom, Kawamuragi (Inchinko), Kansui (Sweet), Kanzo (licorice), Candelilla, Kantou, Canna, Raspberry, Kiwi fruit, Kikarasuuri (Ururone), Kikyo (Kikyo, Bellflower) Root), chrysanthemum (chrysanthemum), catalpa (Azumi), sagebrush (yotei), pheasant (kotomi), kizuta, kidachi aloe, kidachi mint, chinoki, kinada (huangkashiwa), gimnema sylvester, cabbage,
Cabbage immature fruit, cucumber, Kiraja saponaria, Kiraya, kumquat fruit, betel, kinmizukiki (senkaku grass),
Guava fruit, guayule, Quercus infectria (gallicia), cucumber nuts, wolfberry (Gugi, gugi, gugi leaf, skeletal bark), cedar spruce (Tenmen winter), kuzu (kuzukane),
Camphor tree, gooseberry fruit, gardenia, gardenia, blackberry, kumazasa, black pine, horseshoe
(Cranberry), cranberry fruit, chestnut or its astringent skin, curculigo-latifolia fruit, grapefruit fruit, buckthorn, black crocodile (rescue), clove (clove,
Dingka), Kaigai (Epispermia japonica), Celosia (cow flower, Chicken corolla), Bayberry (laurel), Gentiana, Gennoshoko (Old grass), Koshinbara (Monthly flower), Kosuihakka (Melissa), Kozo fruit, Kocha (tea), Kouhone
(River bone), kouhon (straw and kara straw), koulyang, koendro fruit, koganebana (yellow gon), lingonberry (koshibachi), coconut fruit, goshyu (goshuyu), goshoichigo (covered bonsai),
Pepper (pepper), copaiba balsam, coffee beans, kobushi tamshiba magnolia (hot spring), burdock (burdock, burdock), kobotdur, sesame (sesame), sesame grassland (wort), wheat (wheat), rice / rice bran ( Red bran, white bran), rice oil,
Cola acuminata seeds, cola vera seeds, fenugreek fruits, colombo, kondurango, kombu, konjac, comfrey (finnari grass), saxima button pulp (weird sen), cherry leaves, flowers, fruits, bark (cherry bark), Pomegranate, Sasa, Sasasa lily (Yuri), Sazanka, Sadomodaka (Sawashatsu), Sweet potato, Sugar cane, Sugar beet, Sunebunt jujube (Acid jujube), Saffron (Bamboo safflower, West safflower), Pomelo fruit, Sabonsou, Salmonberry fruit, Sarashinashoma ( Masu-mae), Salvia (sage), Sawagikyo (Yamakona), Hawthorn (Yamazako), Sanshin ginseng (Sanjin ginseng), Sanshuyu (sanshuyu), Sansho (sansho), Sandskon (Yamazone),
Shea (carite), cyanoki fruit, shiitake (shiitake), shion
(Shiwon), Red squirrel, Shikunshi (Kimiko), Perilla, Aojiso, Chirimenjiso, Catamendiso (Shiyoha, Shiso), Rosewood, Shinanoki, Chinese forsythia, Lily of the valley
(Chrysanthemum flower), Spiraea, Potato, Peony (Shakuyaku), Shajin (Sasan), Janohige (Motomon winter), Juzudama, Palm fruit, Ginger (Ginger), Shobu (Iris, Iris root), Shozuku fruit, Shirakashi seed, Seeds of white butterfly, white blossoms, white blossoms, white blossoms, white clover, cinnamon, watermelon (watermelon), honeysuckle (gold and silver blossoms, venomous winter), watermelon (acid model), swimberry fruit, strawberry fruit, Horsetail (Thorn), Stevia, Plum fruit, Pinus sylvestris, Larch of Europe, Larch of Europe, Walnut of Europe, Walnut, Hawthorn, Horse chestnut (Maloney),
Pear fruit, papaver rhododendron, papaver rhododendron, papaver rhododendron (milfoil), papaver rhoeas, papaver rhododendron, papaver rhododendron, papaver rhododendron, pear horticulum, pear squirrel , Celery, sendan, senna fruit or leaf, primrose (large tate), souka (grass), buckwheat seed, soybean potato, rhubarb (daihuang), radish, soybean, daidai
(Orange, kikomi), takatodai (daigeki), takarababi (guin), dark sweet cherry fruit, hollyhock, hollyhock thyme (thyme, momoka), hollyhock (tachibana skin), hollyhock willow, tamarind seeds, onion, tamshiba ),
Tara tree or its root bark, tanjin (dansin), dammar, cherry fruit, chiyaya or its root (chinese radish), chikusetsu carrot (bamboo ginseng), chicory, Korean ginseng (Gomiko), Korean daisou (Ohaku), Korean ginseng Ii), Ginseng (carrot), Artemisia (Aiba), Choreimaitake (Irei), Tsukimiso, Camellia, Camellia,
Tsumekusa (lacquer lacustrine), Tsuyukusa (duckling grass), Tsuruazuki (red azuki bean), Tsurudukudami (what's a crow), Tsuruna (apricot), Carrot (four-leaf ginseng), Tsuwabuki, Deiko, Teuchigulumi, Duberry fruit, Tengusa, Tendaiyaku (crow) Medicine), red pepper, winter pepper, red pepper, pepper, touki, marigold, corn or corn hair (nanban hair), red horned mochi (female child), torindo (dragon gall), Dokudami (jujutsu), radish (root sprout), ginseng (bamboo carrot), eucommia (tochu, tochuha), tomato, tragacanth, triacanthus seed, tormentilla, dronoki, trollooi, nizeleria berry fruit, yam
Yamanoimo (Yakuyaku), Nagi-kida (Butcher Bloom),
Koji Naginata, Nazuna, Rape, Natsumikan, Jujube (daijutsu), Naniwaibara (Kanekurako), Narukoyuri (Yellow),
Peanut (peanut), Nanten (Minami Tenmi), Nigaki (Makinoki), Nigamogi (bitumen), Nikuzuku, Nikkei (Cinnamon bark),
Chinese chive (Nirako), Garlic (garlic), Nurde (Fivefold child), Leek,
Noa Thistle (Oogi), Noibara (Fruit), Yarrow, Nodake (Maehu), Nobara, Nomomi leaves, fruit, palm, pineapple fruit, Hibiscus, Hakata lily (Yuri), Hakamauraboshi (Bone crushing), Hakusen ( Ringworm skin), huckleberry fruit, chickweed (red), hazel (haruko),
(Roth root), basil, lotus (lotus, lotus), parsley (holland jelly), hadakamugi, batata, hachiku madake (bamboo-boiled), patchouli, mint (lightly loaded, lightly loaded leaves), adlay (yokuinin), hanasuge ( Chichimo), banana, hanamint, vanilla bins, papaya, lycopodium (rat), hub grass,
Paprika, Hamago and Mitsuba Hamago (vine thorns), Hamasuge (Katsubushi), Hamabishi (Situriko), Hamanas (maikai flower), Hamabofu (beach storm), Hamamerisu, rose (rose), Parietaria, harunire (yuhi, yuyu) White skin, yuba),
Breadfruit, Hiougi (sun-dried), Lycopodium carrot (party party), pecan nut, giganbana (stone garlic, mulberry flower),
Hikiokoshi (prolonged life grass), Hishi (Hishimi), pistachio, beet, hitotsuba (stone reed), hinotainokozuchi (cow knee), hinoki, hiba, castor, sunflower, bell pepper, hinimama (incense blossom), himematsutake, pimenta fruit, juniper , Hiyu fruit, below mallow, Hiroha okinagusa (white head), loquat
(Loquat, loquat leaf), betel nut (large clothing skin, betel palm), squash (southern rattan), butterflies and dandelions (winter flowers, winter leaves),
Fujibakamama (Ranbusa), Fujimame (tonboshi), grape fruit or pericarp and leaves, beech, Fuyushinatsukutaketake (Cordyceps sinensis), Brazilian liquorice, black currant fruit, blackberry, plum fruit, flucellaria, blueberry (Acacia catechu), Prunes, blond psyllium, bundo (green beans), loofah, safflower (safflower), belladonna, berry fruit, boysenberry fruit, boufu (windbreak), spinach, physalis (Torone), honoki (thick, sumpaku), bokeh (Gourd), Hosoba-naokera (blue Ajutsu), Hosobabarengiku, Bodaiju (Bodai tree), Button (peony, peony skin),
Jojoba, White berry fruit, Hongshimeji, Mesulten Nanshaw (Tennan star), Ephedra (Masochism), Macadamia nut, Macri (Martian grass), Magwa (Mulberry leaf), Madake (Bamboo boiled),
Matatabi (Kiten Tate), Matsukasa, Matsuba, Matsuhodo (Bukuryo),
Marjoram (Hanaminka), Malvanozadin (Bitterfruit), Mulberry fruit, Quince, Marronnier, Mango, Mangosteen, Manshugulumi, Mandarin fruit, Mannentake (Lingzhi), Fruits of Citrus genus (Komi), Mishimasaiko (Saiko), Mizokashi ( Hanabe Lotus), Misohagi (Chisenbana), Mitsugashiwa, Mitsuba, Mitsubahamago (Tenshiko), Midorihakka, Mimosa, Myoga, Miracle Fruit Fruit, Myrrh, Milobalan, Mukunoki, Mukuroji, Murasaki (purple root), Purple Sorghum, Swordfish (meadow grass), scallop, melaleuca, melissa, melilot, melon fruit, sagebrush, sagebrush, mocktail (wood incense), maple smelt, peach leaf (peach leaf), fruit, sprouts, morello cherry fruit, morroheya (yellow), Yakawamurasakiimo, yakuchi (masuchi), cornflower Chrysanthemum) ,, Yagurumahakka, Alnus firma (vane wheel), Yachiyanagi, Fatsia Japonica
(Octagonal gold plate), mistletoe (parasitic willow), willow leaf, yabugarashi, yabukouji (purple gold beef), pokeweed (commercial land), yamahanoki (yamaharu), yamamomo (yangmei), yamakomogi, eucalyptus, yukinoshita (tiger ear) Grass), Yucca flebifolia, Yuzu fruit, lily, yogurusa, mugwort (mushroom leaf), lime fruit, rye, lacanca fruit, raspberry fruit, lavender, longan (longan meat), ryocha (green tea), apple fruit, gentian, rubus suavisimus (Sweet beet), lettuce, red currant fruit, lemon fruit, lemongrass, forsythia (forsythia), astragalus, wax palm, loganberry fruit, logwood, rosemary (mannenrow), rosehip (nobara), wakame, wasabi, Waremokou (chiyu) and the like.

Examples of the seaweeds include seaweeds [green algae: Chlorella vulgaris, Chlorella pyrenoidosa, Chlorella ellipsoidia, Usbaaonori, Suziahonori, Hiraaonori, Bouuaonori, Hosoedaoonori], and seaweeds [Brown algae: Macombum, Lyconib, Hosoibu] Honey kelp, wakame, hirome, blue seaweed, macrocystis pilifera, macrocystis integrifolia, neocitys luetokeana, hijiki, hibamata], seaweed [red algae: red alga: jijirimen, maxa (tengusa), hirakusa, onyxa, obaxa, kataobaxa ,
Yatabegusa, Yuikiri, Shimatengusa, Tosakanori, Togekirinsai, Akumasakirinsai, Kirinsai, Byaksinkirinsai, Tsunomata, Obatsunomata, Tochaka (Yahatsunomatata), Ezotunomata, Togetsunomata, Hirakotonogi, Kotoji, Kotoji , Heius Banori] and the like.

Other algae, for example, green algae (Chlamydomonas: Chlamydomonas, Akayukimo, Dunaliella: Dunaliella, Chlorococcus: Chlorococcus, Kwanomimo: Kwanomimo, Volvox: Ohigewari, Volvox, Palmera, Yotsumemo, Spirogyra: Hizaori, Spirogyra, Tulsimidro, Hibimidro: Hibimidoro, Aosa: Anaoasa, Amiosa, Nagaosa, Kawanori: Kawanori,
Genus Fricciella, genus genus: medusa, asamidori ogusa, kawashiogusa, marimo, valonia: tamagobaronia, tamabaronia, magnum: tamatamamo, iwata: husaiwata, suricogita, heraiwirata, crocodile, crocus Sakibutimimil, Nagamil, Hiramil, and Casanori:
Casa nori, Juzumo
Species of the genus Mizudzumo, Genus genus, Colecaetus, Genus genus genus, Species of the genus Rosa: Rosa spp. Genus (Suizenjinori: Suizeninori, Aokko, Nostoc: Kawatake, Ishikurage, Hassai, Yuremo, Racenmo (Spirulina): Spirulina,
Genus trichodesmium (Aiakashio)), brown algae (Piraella spp .: Pyraella spp., Shiomidoro spp .: Nagamioshidoro, Isogrape spp .: Isogrape, Isogawara spp .: Isogawara, Krogashira spp .: Gunsenkurogashira, Kasilaki spp .: Kashirazaki, Muchimo spp .: Muchimo, Hiramchimo, Kebelligsa, Amidixa: Amidixa, Sakibiroamiji,
Sanadagusa: Sanadagusa, Fukurinamiji, Commongusa: Commongusa, Yahuzugusa: Ezoyahazu, Yahuzugusa, Uraboshiyahazu, Jingamigusa: Jigamigusa, Umiuchiwa: Genus Umiuchiwa, Konamiumichusawa, Akabaumikawasuna Genus Nabarimo, Chasoumen spp. , Mozuku: Mozuku, Ishige: Ishige, Irolo, Icmegasa: Ichimegasa, Carapacea: Callidia, Umibos: Umibos, Urushigusa: Urushi Sa, Keurushigusa, Tabakogusa, Konbumodoki genera: Konbumodoki, Habamodoki genera:
Genus Habanomoki, Genus Habanori: Havanori, Genus Habanori: Habanori, Common Bukuro: Common Bukuro, Ezobukuro: Ezobukuro, Fukuronori: Fukuronori, Watamo, Chishimafukuronori Genus: Genus Genus, Kagomenorimura, Genus Kamuramemura ： Genus Shark, Genus Genus: Genus Genus, Genus Genus: Genus Genus, Genus Genus: Genus Lily, Genus Genus: Genus Genus, Genus Genus:
Turmo, Anatome: Aname, Siberium: Sujime, Misujikombu: Misjikombu, Atsubamisujikombu, Kombu: Gatsugarakomubu, Oysterfish, Onicombu, Goheikombu, Nagakombu, Pea, Ootomikombu, Torokomokuto: Torokontokuto , Genus genus: swordfish, swordfish, black squid, genus scorpionum: squirrel, squirrel genus: squirrelcomb, kuroshiome: kuroshiome, scorpion genus: feline sacion, alame genus: alame, Ainuokame: Ainuwakame, squid, squid Ezoishige, Yabamoku: Yabamoku, Rappermoku: Rappermoku, Jiromoku: Uganomok,
Joromok, Hyemok, Hondawara: Tamanashimoku,
Isomok, Nagashimamoku, Akamoku, Shidamoku, Hondawara, Nejimoku, Narasamo, Mametara, Tatsukuri, Yasumatamoku, Umitranoo, Oobamoku, Fushizushimoku, Hahakimoku, Togemoku, Jolemoku, Sawtooth moku,
Obanokogirimoku, Sugimoku: Sugimoku, Ukimo: Oukimo, Buruquimo: Burukimo, Kayamonoli: Kayamonori, etc., Red algae (Ushikenori: Ushikenori, Funorinoushike, Amanori: Asakusanori,
Susabinori, Uppluinori, Onimanori, Tasa, Filitasa, Benitasa, Rhodocorton spp. Pine genus:
Pine L., Fusanori: Fusanori, Nisefusanori: Nisefusanori, Sodegarami: Sodegarami, rattle:
Rattle rattle, rattle rattle, broad-spotted genus: broad-spotted scorpion, scorpion genus: small-spotted squirrel, genus Kagikenori: Kaginori, Kakenenori, Tengusa genus: Himetengusa, Hytengusa, Oobusa, Nambugusa, Kohido, Kohira, Kojira, Ohihime, Kohido, Kouhira, Ohihime ： Genus genus: genus genus: genus genus: genus ryegrass, genus ryumunsou, genus rhyme, genus rhinoceros, genus nisekarekigusa genus: genus foxes, genus okitsubara: genus syllabus, genus akaba: genus akaba, malvagana, sagogana : Genus Shiogosagoromo, Genus Iwanoka: Genus Etsukiiwanokawa, Genus Kinokawa: Genus Kinokawa, Genus Kaninote: Genus Kaninote, Coral: Coral genus, Mucadenori: Mucadenori, Suzukade, Catanori, Hiramukade, Kyounohimo, Sakuranori, Nikumukade, Tambanori, Crane, Isonohana: Nuraksa, Cronulaxa, Omodenori, Hirakintoki: Hirakintoki
Genus: scorpionfish, genus: serrata,
Kintoki, Matsunori, Komenori, Tosukamatsu, Atsumatsu, Kakurite spp. Genus: genus genus: genus genus: quinuhada; Species of Susqueke Beni: Species of Suska Kebeni, Species of Okamuragusa: Yamadagusa, genus of Mirin: Mirin,
Hosobamilin, Tosakanori: Kikutosaka, Ezonameshi: Ezonameshi, Isomokka: Isomokka, Yukari: Yukari, Hosoyukari, Ibaranori: Ibaranori,
Rhinoceros rose, Tachiibara, Kagibaranori, Kijinoo genus: Chidinooo, Isodanthus: Isodanthu, Atsubanori genus: Atsubanori, Ogonori: Ogonori, Tsurushiramo, Silamo, Ogonori, Mizokonori, Kabanori,
Genus genus: beetle scrophulari, fushikrenori genus: fushikrenori, namitake mushroom: namiitake mushroom, sponge genus: sponge beetle, okinori genus: okitsunori, saimi genus: itanigusa, simi, harugane,
Lotus spp .: Lotus spp.
Hosoibonori, Nobonori, Scrophulariaceae: Kurohaginnansou, Akabaginansou: Akabaginnansou, Hishibukuro: Hishibukuro, Madaragusa: Togemadara, Ekimadara, Taoyanagisou: Hanagusa ： Hanaedae, Hirata goats ：
Hirata goat, genus Darus: dars, masagoshibari, anadarus, eubagusa genus: eubagusa, Benifukuronori genus: Benifukukuronori, fushitsunagi: fushitsunagi, himefushinushigi, hirohafushinagi, kawanatsugisugisai, genus tsutsugigigi , Honey Negis, Amixa, Egonori: Egonori, Futigis, Saeda: Saeda,
Chrysanthemum spp .: Chrysanthemum spp. Genus: Aristolochia, Ayanishi, Genus: Ayanishi,
Genus Ayaginu: Genus Ayaginu, Genus Dadia: Enashidazia, Genus Shimadada: Isohagi, Shimadadia, Genus Dazimodoki: Dazimomodoki, Itogusa: Moletogusa, Futigusa, Macri Genus: Makri, Sanaginori: Hanayanagi, Yuna, Yanaginori, Motsunagi
Sasabayanaginori, genus Sozo: Closozo, Kobusoso, Hanesozo, Sozonohana, Hanegusa: Hanegusa, Kehanegusa, Kozanemo genus: Kozanemo, Isomurasaki, Hosokozanemo, Himegoke genus: Himegoke, Kurohimegoshi, Hioudoogushigu , Genus genus: genus cypress, genus genus: genus isola, genus genus: genus genus: genus genus, genus syllabus: genus sylvestris, genus sculpture: genus sculpture: genus genus, genus genus, and : Chinolimo, Chisinori: Chisinori, etc., and axle algae (Shajikumo, Shiratamamo, Hossiturimo: Hossiturimo, Lichnotatum, Fussurimo) Rascomo genus: Himeimo moth, Chabo hiramomo, Tripera genus, etc., yellow algae (Hikarimo genus: Hikarimo etc.) and the like.

[0045] Materials derived from animal raw materials include a cockscomb extract, a cow / human placenta extract, a pig / cattle stomach, a duodenum or an intestinal extract or a decomposition product thereof, and a pig / cattle spleen extract. Products or their degradation products, extracts of swine or bovine brain tissue, water-soluble collagen, collagen derivatives such as acylated collagen, collagen hydrolysates, elastin, elastin hydrolysates, water-soluble elastin derivatives, keratin and its decomposition products Or a derivative thereof, a silk protein and its degradation product or a derivative thereof, a pig / bovine blood cell protein degradation product (globin peptide), a pig / bovine hemoglobin degradation product (hemin, hematin, heme, protohem, heme iron, etc.), milk , Casein and its decomposition products or their derivatives, skim milk powder and its decomposition products or their derivatives, lactoferrin or its decomposition products, chicken egg ingredients Such as fish meat decomposition products.

(14) Microbial culture metabolite Yeast metabolite, yeast extract, rice ferment extract, rice bran extract, euglena extract, lactic acid fermentation product of skim milk powder, trehalose or a derivative thereof.

(15) α-hydroxy acid Glycolic acid, citric acid, malic acid, tartaric acid, lactic acid and the like.

(16) Inorganic pigments Silicic anhydride, magnesium silicate, talc, kaolin,
Bentonite, mica, titanium mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide,
Titanium oxide, calcium carbonate, magnesium carbonate, yellow iron oxide, red iron oxide, black iron oxide, gunjou, chromium oxide,
Chromium hydroxide, carbon black, calamine, etc.

(17) UV absorber p-aminobenzoic acid derivative, salicylic acid derivative, anthranilic acid derivative, coumarin derivative, amino acid compound,
Benzotriazole derivatives, tetrazole derivatives, imidazoline derivatives, pyrimidine derivatives, dioxane derivatives, camphor derivatives, furan derivatives, pyrone derivatives,
Nucleic acid derivatives, allantoin derivatives, nicotinic acid derivatives,
Vitamin B ₆ derivatives, oxybenzone, benzophenone, guaiazulene, shikonin, baicalin, baicalein, berberine and the like.

(18) Astringent succinic acid, allantoin, zinc chloride, zinc sulfate, zinc oxide, calamine, zinc p-phenolsulfonate, potassium aluminum sulfate, resorcinol, ferric chloride, tannic acid (including catechin compounds) Such.

(19) Antioxidants ascorbic acid and its salts, stearic acid ester, tocopherol and its ester derivatives, nordihydroguaseletenic acid, butylhydroxytoluene (BHT),
Butylhydroxyanisole (BHA), hydroxytyrosol, parahydroxyanisole, propyl gallate, sesamol, sesamolin, gossypol and the like.

(20) Anti-inflammatory agent Ictamole, indomethacin, kaolin, salicylic acid, sodium salicylate, methyl salicylate, acetylsalicylic acid, diphenhydramine hydrochloride, d or dl-
Camphor, hydrocortisone, guaiazulene, camazulene, chlorpheniramine maleate, glycyrrhizic acid and its salts, glycyrrhetinic acid and its salts and the like.

(21) Disinfectant / Disinfectant Acrinol, sulfur, benzalkonium chloride, benzethonium chloride, methylrosaniline chloride, cresol, calcium gluconate, chlorhexidine gluconate, sulfamine, mercurochrome, lactoferrin or a hydrolyzate thereof.

(22) Humidifier Glycerin, propylene glycol, 1,3-butylene glycol, hyaluronic acid and its salts, polyethylene glycol, chondroitin sulfate and its salts, water-soluble chitin or chitosan derivatives, sodium lactate and the like.

(23) Hair preparations Selenium disulfide, alkylisoquinolinium bromide solution, zinc pyrithione, biphenamine, thianthol, castal tincture, ginger tincture, capsicum tincture, quinine hydrochloride, strong aqueous ammonia, potassium bromate, bromate Sodium, thioglycolic acid, etc.

(24) Flavors Natural animal flavors such as musk, civet, castorium, ambergris, anise essential oil, angelica essential oil, ylang ylang essential oil, iris essential oil, fennel essential oil, orange essential oil, cananga essential oil, caraway essential oil, cardamom essential oil, Guayakwood essential oil, cumin essential oil, black letter essential oil, cinnamon essential oil, cinnamon essential oil, geranium essential oil, copaiba balsam essential oil, coriandel essential oil, perilla essential oil, cedarwood essential oil, citronella essential oil, jasmine essential oil, gingergrass essential oil, cedar essential oil, spearmint essential oil, western Mentha essential oil, Great Fengxiang essential oil, Tuberose essential oil, T-shaped essential oil, Orange flower essential oil, Winter green essential oil, Truval sam essential oil, Batturi essential oil, Rose essential oil, Palmarosa essential oil, Cypress essential oil, Hiba essential oil,
Sandalwood essential oil, Petitgren essential oil, Bay essential oil, Vetiva essential oil, Bergamot essential oil, Peruvian balsam essential oil, Boad rose essential oil, Camphor essential oil, Mandarin essential oil, Eucalyptus essential oil, Lime essential oil, Lavender essential oil, Linaloe essential oil, Lemongrass essential oil, Lemon essential oil, Rosemary Vegetable flavors such as essential oils and Japanese peppermint essential oils, and other synthetic flavors.

(25) Dyes / colorants Red cabbage dye, red rice dye, madder dye, anato dye, squid dye, turmeric dye, orange dye, krill dye, persimmon dye, caramel, gold, silver, gardenia dye, corn dye , Onion pigment, tamarind pigment, spirulina pigment, buckwheat whole plant pigment, cherry pigment, laver pigment, hibiscus pigment, grape juice pigment, marigold pigment, purple potato pigment, purple yam pigment, lac pigment, rutin, etc.

(26) Sweeteners Sugar, sweet tea, fructose, arabinose, galactose, xylose, mannose, maltose, honey, glucose, miraculin, monelin, licorice extract and the like.

(27) Nutrition enhancer Shell calcined calcium, cyanocorabamine, yeast, wheat germ, egg yolk powder, hemicellulose, heme iron and the like.

(28) Dairy products Milk, cheese, fresh cream, butter, margarine, powdered milk, whey, condensed milk and the like.

In addition, humectants, hormones, sequestering agents, pH adjusters, chelating agents, preservatives / anti-binders, cooling agents, stabilizers, emulsifiers, animal and vegetable proteins and their decomposed products, animal and vegetable Vegetable polysaccharides and their degradation products, animal and vegetable glycoproteins and their degradation products, blood flow enhancers, anti-inflammatory and anti-allergic agents, cell activators, keratolytic agents, wound healing agents, foam enhancers, thickeners Agents, oral preparations, deodorants / deodorants, bitters, seasonings, enzymes, and the like can be used, and additive and synergistic effects can be expected by using them in combination.

The fibroblast proliferation-promoting agent of the present invention, as well as the external preparation for skin and bath, and the food and drink can be in any dosage form, such as ampules, capsules, powders, granules, pills and tablets. , Pharmaceuticals such as solid, liquid, gel, foam, emulsion, cream, ointment, sheet, etc., quasi-drugs,
It can be used by blending it into cosmetics for skin and hair, bath preparations and foods and drinks.

[0063] Specifically, for example, basic preparations such as medicinal preparations for internal and external use, lotions, emulsions, creams, ointments, lotions, oils, packs, etc., facial cleansers and skin cleansers, shampoos, rinses and hair treatments , Hair creams, pomades, hair sprays, hair styling products, perm agents, hair tonics, hair dyes, hair growth and hair growth products, and other hair cosmetics
Makeup cosmetics such as foundation, white powder, whitening, lipstick, blusher, eyeshadow, eyeliner, mascara, eyebrows, eyelashes, etc.
Includes perfumes, bath agents, other toothpastes, mouth fresheners / gargles, liquid odor / deodorant inhibitors, sanitary products, sanitary cotton, wet tissues, etc., and also used in general foods and drinks It is possible.

In addition, the almond, dandelion, crocodile, senkyu, assembly, soybean sprouts, tannin, carrot, hops, mugwort, fibroblast growth promoter containing extract of yokuninin, skin external preparation and bath preparation, food and drink of the present invention May be added in advance or may be added during production, and may be appropriately selected in consideration of workability.

[0065]

EXAMPLES The present invention will now be described with reference to production examples, test examples, and prescription examples, but the present invention is not limited to these examples.

(Production Example 1) 100 g of almonds, dandelion, sambucus nigra, senkyu, assembly, soybean sprouts, carrots, carrots, hops, mugwort, and yokunin were each extracted by heating with purified water (about 80 ° C.) for about 5 hours. After filtration, about 1.0 kg of an extract (dry solid content: about 0.01 to 10.0% by weight) is obtained.

(Production Example 2) 100 g of each of almond, dandelion, European elderberry, primrose, assembly, Sorrel albino, carrot, carrot, hop, mugwort, and yokunin, were either a 50% ethanol solution or a 30% 1,3-butylene glycol solution, or It is immersed in a 60% propylene glycol solution or purified water (about 30 ° C.), extracted at room temperature for 5 days and nights, and then filtered to obtain an extract (dry solid content: about 0.01 to 1).
0.0% by weight).

(Test 1) Fibroblast proliferation promotion test The present inventor used the MTT based on normal fibroblasts of the skin.
Using the reduction method, the effect of promoting cell growth was tested and evaluated.

(Test Method) The MTT reduction method is a method for evaluating cytotoxicity and cell activation (rapid color quantification of growing and viable cells: Tim Mosmann; Journal of Immunological Met).
hods p55-63 (1983)) and MTT (3- (4,
5-dimethyl-2-thiazolyl) -2,5-diphenyltetrazolium br
omide) is taken up by cells, is reduced and cleaved by NADH present in mitochondria in the cells, and utilizes the property of producing water-insoluble blue formazan.This blue formazan is dissolved in acidic isopropanol, and the absorbance is measured. It is to be measured. That is, since the amount of blue formazan is expressed as the absolute amount of mitochondria, the amount of blue formazan that changes over time was measured, and the cell proliferation rate was calculated.

(Quantification of Fibroblast Proliferation-Promoting Activity) The present inventor has proposed that FBS in which cells do not grow or decrease in advance.
(Fetal calf serum) The concentration was examined and MEM containing 0.5% FBS
It was confirmed that this condition was satisfied when cultured in a medium, and the serum concentration of the test medium was adjusted to 0.5%, and it was decided that the fibroblast proliferation promoting effect of only the test sample could be quantitatively evaluated.

(Measurement of fibroblast proliferation promoting action) 5% F
Using a MEM medium containing BS (fetal calf serum), human dermal fibroblasts (NB1RGB purchased from RIKEN Genebank / Cell Development Bank) at a density of 5 × 10 ³ Cell / Well for 96 Wells pl
ate and cultured for 24 hours under the conditions of 5% CO ₂ and 37 ° C.
Thereafter, the sample was replaced with a MEM medium containing 0.5% FBS to which samples of each concentration (Production Example 1: various plant extracts) were added. At this time, the blank was a MEM containing 0.5% FBS containing no sample.
Change the medium to the same medium every 3 days for 9 days
Culture was continued in a CO ₂ incubator, and the absorbance difference between 570 nm and 620 nm was measured by the MTT reduction method. At this time, the absorbance difference of the blank was set to 100, and the cell growth rate of the test sample-added group with respect to the difference was measured.

[0072]

FIG.

[0073]

FIG. 2

[0074]

FIG. 3

(Test Results) As shown in FIGS. 1-3, the extracts of almonds, dandelions, elderberry, sunflower, siberium, soybean sprouts, tonin, carrot, hops, mugwort, and yokuninin of the present invention have a fibroblast proliferation promoting effect. That was confirmed.

(Test 2) Safety test (1) Primary skin irritation test Various plant extracts obtained in Production Examples 1 and 2 were prepared in purified water so as to have a dry solid concentration of about 0.5% by weight. Japanese rabbits with female back shaved (female, 3 per group, weight 2.3kg)
Before and after). The judgment was made 24, 48, and 72 hours after application by the primary irritation scoring method using erythema and edema as indices. The result was negative in all animals without any erythema and edema.

(Test 3) Safety test (2) Cumulative skin irritation test Similarly, various plant extracts obtained in Production Examples 1 and 2 were added to purified water so that the dry solid concentration was about 0.5% by weight. Guinea pigs (female,
Once a day, 5 times a week on the skin of 3 animals per group, weight around 320g)
0.5 ml / animal was applied. Application was performed over two weeks and shaving was performed on the last application day of each week. Judgment was made on the day of each application and on the day after the last application using the primary irritancy scoring method using erythema and edema as indices. As a result, all animals were judged as negative without any erythema and edema over 2 weeks.

(Test 4) Safety test (3) Acute toxicity test Similarly, various plant extracts obtained in Production Example 1 were concentrated under reduced pressure.
The dried powder was fasted for 4 hours before the test.
2,000m in mice (male and female, 5 mice per group, 5 weeks old)
g / kg was orally administered, and the appearance and degree of toxicity symptoms were observed over time. As a result, no abnormalities were observed in all the mice for 14 days, and no abnormalities were observed in the dissection results. Therefore, the LD ₅₀ was determined to be 2,000 mg / kg or more.

(Prescription examples) Production of various fibroblast cell growth agents, skin external preparations and bath preparations The following formulation examples are shown below according to the above evaluation results, and each formulation example is produced by a conventional method in the production of each product. And only the amount was shown. Further, the present invention is not limited to these.

(Formulation Example 1) Emulsion weight% 1. Squalane 5.0 2. Olive oil 5.0 3. Jojoba oil 5.0 4. Cetyl alcohol 1.5 5. Glycerin monostearate 2.0 6. Polyoxyethylene (20) cetyl ether 3.0 7. Poly Oxyethylene (20) soorbitan monooleate 2.0 8.1,3-butylene glycol 1.0 9. Glycerin 2.0 10. A: Almond hot water extract B: Atlantic dandelion hot water extract C: Senkyu 20% ethanol extract D: Assembly 30 % 1,3-butylenglycol extract E: somakumoku hot water extract F: tounin 20% anhydrous ethanol extract G: sambucus 30% ethanol extract H: carrot 20% 1,3-butylenglycol extract I: hop 30% ethanol extract J: 30% rosewood extract, Kokumitsu 30% ethanol extract K: Yokuinin 30% ethanol extract * Any one of AK extract 5.0 11. Appropriate amount of fragrance and preservative

(Formulation Example 2) Lotion weight% 1. Sorbit 2.0 2.1.3-butylene glycol 2.0 3. Polyethylene glycol 1000 1.0 4. Polyoxyethylene oleyl ether (25E.O.) 2.0 5. Ethanol 10.0 6. Almond heat Water extract 3.0 7.pH adjuster qs 8.preservative qs 9.Purified water 100 residue

(Formulation Example 3) Peel-off pack (% by weight) 1. Glycerin 5.0 2. Propylene glycol 4.0 3. Polyvinyl alcohol 15.0 4. Ethanol 8.0 5. Polyoxyethylene glycol 1.0 6. European dandelion 20% 1,3-butylene glycol extract 4.0 7. Appropriate amount of fragrance and preservative

(Formulation Example 4) Cold cream weight% 1. Salicone beeswax 11.0 2. Liquid paraffin 22.0 3. Lanolin 10.0 4. Almond oil 15.0 5. Borax 0.5 6. Ethanol 30% ethanol extract 2.0 7. Perfume, preservative Adequate amount 8.Purified water 100 residue

(Formulation Example 5) Shampoo wt% 1. Triethanolamine lauryl sulfate 5.0 2. Sodium polyoxyethylene lauryl ether sulfate 12.0 3.1,3-butylene glycol 4.0 4. Diethanolamide laurate 2.0 5. Disodium edetate 0.1 6. Assembly hot water extract 5.0 7. Appropriate amount of fragrance and preservatives 8. Residual to make purified water 100

(Formulation Example 6) Body soap weight% 1. Potassium laurate 15.0 2. Potassium myristate 5.0 3. Propylene glycol 5.0 4. 30% anhydrous ethanol extract in soybean ash 7.0 5. Appropriate amount of pH adjuster 6. Appropriate amount of preservative 7. Residual with 100 purified water

(Formulation Example 7) Rinse weight% 1. Stearyltrimethylammonium chloride 2.0 2. Cetostearyl alcohol 2.0 3. Polyoxyethylene lanolin ether 3.0 4. Propylene glycol 5.0 5. Hot water extract of tounin 4.0 6. pH adjuster Appropriate amount 7.Preservatives Appropriate amount 8.Purified water 100 residue

(Formulation Example 8) Hair liquid weight% 1. Ethanol 29.0 2. Polyoxypropylene butyl ether phosphoric acid 10.0 3. Polyoxypropylene monobutyl ether 5.0 4. Triethanolamine 1.0 5. Eel extract of 30% Sambucus nigra 30% ethanol 2.0 6 .Carrot hot water extract 3.0 7. Preservative appropriate amount 8. Purified water 100 residue

(Formulation Example 9) Hair tonic weight% 1. Ethanol 40.0 2. Ethyl oleate 1.0 3. Polyoxyethylene (40) hydrogenated castor oil 2.0 4. A: Almond 20% ethanol extract B: Dandelion dandelion hot water extraction Liquid C: Extract of hot water from Senkyu D: Extract of 30% phyllopolyphenol recall E: Extract of 40% ethanol of Sophora serrata F: Extract of hot water from Tonin G: Extract of 20% ethanol from Elderberry H: Extract of hot carrot water I: Hot water extract from hops J: 50% ethanol extract from Mukugum K: Hot water extract from Yokuinin ※ Extract of any one of AK 5.0 5. Preservatives qs 6. Purified water 100

(Formulation Example 10) Granule bath agent weight% 1. Sodium bicarbonate 61.0 2. Anhydrous sodium sulfate 35.0 3. Borax 3.0 4. Hops hot water extract 3.0 5. Musket hot water extract 3.0

(Prescription example 11) Coating protective agent A gauze or liniment cloth is impregnated with a prescription solution prepared by mixing an appropriate amount of a mixed solution of an elderberry / Yoquinin with hot water, an antibiotic, an anti-inflammation, and attached to the wound. Alternatively, the chlorella extract may be directly sprayed locally and covered with gauze or the like.

(Prescription Example 12) Juice drink weight% 1. Glucose liquid sugar 33.0 2. Grapefruit juice 60.0 3. Fragrance 1.0 4. Sambucus elderberry hot water extraction powder 3.0 5. Carrot hot water extraction powder 3.0 6. Acidulant proper amount

(Prescription example 13) Mentsuyu liquid wt% 1. Soy sauce 76.0 2. Black vinegar 2.0 3. Glucose 15.0 4. Sodium glutamate 3.0 5. Assembly hot water extraction powder 1.0 6. Sawberry hot water extraction powder 2.0 7. Tonin heat Water extraction powder 1.0

(Formulation Example 14) Udon or buckwheat weight% 1. Flour or buckwheat flour 93.0 2. Salt 1.0 3. Mukuge 50% water extract 3.0 4. Yokuinin hot water extract 3.0

(Formulation Example 15) Bread wt% 1. Wheat flour 88.5 2. Salt 0.5 3. Glucose 6.5 4. Almond hot water extraction powder 1.5 5. Dandelion hot water extraction powder 1.5 6. Assembly hot water extraction powder 1.5

(Formulation Example 16) Cookie weight% 1. Milk 62.0 2. Whole egg 14.0 3. Sugar 15.0 4. Constarch 5.0 5. Salt 0.5 6. A: Hot water extract of almond B: Hot water extract of dandelion C: Hot water extract of Senkyu D: Hot water extract of Japanese cabbage E: Hot water extract of Sophora serrata F: Hot water extract of Ginseng G: Elderberry hot water extract H: Carrot hot water extract I: Hop hot water extract J: Mukuge hot water extract K: Yokuinin hot water extract * Extract of any one of AK 3.5 7. Appropriate amount of fragrance

(Test 5) Effect test of use The almond, dandelion, elderberry, Sengoku, assembly, Sahakuhi, Tonin of the present invention,
The effects of using a skin external preparation and a bath preparation containing a carrot, hop, mugwort, and yokunin extract were examined. The test used is to worry about dry skin, rough skin, eczema, atopic dermatitis and other skin diseases.
Twenty 50-year-olds are panelists, twice daily, morning and night,
After washing the face, an appropriate amount of the emulsion of Formulation Example 1 was applied to the face for 3 months. Also, for 20 people (5 to 50 years old) who have the same skin disease on the scalp and hairline, apply the appropriate amount of the hair tonic of Formulation Example 9 to the scalp for 2 months after daily hair washing. The use test was carried out by.

Further, the bath preparation of Formulation Example 10 was also applied to 20 people aged 1 to 65 years old who suffer from skin diseases such as dry skin, rough skin, eczema, and atopic dermatitis. For a month, the children were immersed in bath water in which an appropriate amount of bath agent was dissolved once a day, and a use test was conducted. As controls, those prepared by removing the various plant extracts of the present invention from milky lotions, hair tonics, and bath preparations by the same method were used. The evaluation was performed according to the following criteria. The results are as shown in Tables 1-3, and the numbers in the table represent the number of persons. No one complained of abnormalities on the skin or scalp during the use period.

"Skin (scalp) disease improving effect" Efficacy: Redness, itching, dry skin, and rough skin due to inflammation such as eczema were improved. Somewhat effective: Redness and itching, dry skin, and rough skin due to inflammation such as eczema were slightly improved. Ineffective: No change from before use.

(Test Results) As is clear from the results shown in Tables 1-3, an external preparation for the skin containing the almond, dandelion, sambucus nigra, senkyu, assembly, soba sap, tonin, carrot, hops, sycamore and yokuninin extract of the present invention And the use of bath agents may cause inflammation due to eczema,
A favorable effect was confirmed on the improvement of skin diseases such as itching, dry skin and rough skin.

[0100]

[Table 1]

[0101]

[Table 2]

[0102]

[Table 3]

(Test 6) Effect test of use The almonds, dandelion, elderberry, Sengoku, assembly, Sahakuhi, Tonin of the present invention,
The effect of using a food containing carrot, hop, mugwort, and yokuinin extracts was examined. The test used was a panel of 10 people aged 5 to 50 who were suffering from skin disorders such as dry skin, rough skin, eczema, and atopic dermatitis. We had you drink for three months.

As a control, a cookie prepared by removing the plant extract of the present invention from a cookie in the same manner was used. The evaluation was performed according to the following criteria. The results are as shown in Table 1 and the numerical values in the table represent the number of persons. No one complained of abnormalities during the use period.

"Effect of improving skin (scalp) disease" Efficacy: Redness, itching, dry skin, and rough skin due to inflammation such as eczema were improved. Somewhat effective: Redness and itching, dry skin, and rough skin due to inflammation such as eczema were slightly improved. Ineffective: No change from before use.

(Test results) As shown in Table 4, the results are shown in Table 4. The almond, dandelion, elderberry,
Use of foods containing extracts of Sengoku, Assemblage, Sahakuhi, Tonin, Carrot, Hops, Mukuge, and Yokuinin has been confirmed to be effective in improving skin diseases such as inflammation due to eczema, itching, dry skin, and rough skin. Was done.

[0107]

[Table 4]

[0108]

According to the present invention, one or more plant extracts selected from almonds, dandelion, elderberry, primrose, assembly, sorrel, tounin, carrot, hop, mugwort, and yokuinin promote fibroblast proliferation by normal cells. It is clear that it has a remarkable effect, and furthermore, it does not show cytotoxicity, so that it is safe to use internally or externally for humans or animals. Therefore, it can be used as a fibroblast growth promoter, eczema, rough skin,
Skin / scalp, oral cavity, eyes, ears, nose, anus, genital area for the purpose of skin dryness, skin diseases such as atopic dermatitis, skin and dermal growth promotion, skin aging prevention, and wound healing promotion It is effective in preventing and treating various traumas. Furthermore, it can be applied to all forms of pharmaceuticals (pharmaceuticals, quasi-drugs, cosmetics, foods).
When used as a skin external preparation, bath preparation or food or drink, it can be used for the prevention and improvement of skin and scalp such as inflammation due to eczema, itching, rough skin and rough skin.

[Brief description of the drawings]

FIG. 1 shows the relationship between the concentration in the medium and the cell growth rate when the almond, dandelion, sambucus nigra, and rosewood extracts of the present invention are added.

FIG. 2 shows the relationship between the concentration in the medium and the cell proliferation rate when the extract of the present invention, the soybean scab and the tonin extract are added.

FIG. 3 shows the relationship between the concentration in the medium and the cell growth rate when the carrot, hop, rose, and yokunin extracts of the present invention were added.

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[Procedure amendment]

[Submission date] August 27, 1996

[Procedure amendment 1]

[Document name to be amended] Statement

[Correction target item name] 0089

[Correction method] Change

[Correction contents]

(Formulation Example 10) Granule bath agent weight% 1. Sodium bicarbonate 61.0 2. Anhydrous sodium sulfate 30.0 3. Borax 3.0 4. A: Almond 20% ethanol extract B: Common dandelion hot water extract C: Senkyu Hot water extract D: Assemble 30% Fluorescent liquid extract E: Sophora oleracea 40% ethanol extract F: Tonin hot water extract G: Atlantic elderberry 20% ethanol extract H: Carrot hot water extract I: Hop hot water extract Liquid J: 50% Euglena extract, K: Yokuinin hot water extract * Any one of AK extract 6.0

[Procedure amendment 2]

[Document name to be amended] Statement

[Correction target item name] 0090

[Correction method] Change

[Correction contents]

(Prescription example 11) Coating protective agent A gauze or liniment cloth is impregnated with a prescription solution prepared by mixing an appropriate amount of a mixed solution of an elderberry / Yoquinin with hot water, an antibiotic, an anti-inflammation, and attached to the wound. Alternatively, the elderberry extract may be directly applied locally and covered with gauze or the like.

──────────────────────────────────────────────────の Continuation of the front page (51) Int.Cl. ⁶ Identification number Agency reference number FI Technical display A61K 35/78 A61K 35/78 W ADA ADAC A21D 2/36 A21D 2/36 A23G 3/00 102 A23G 3/00 102 A23L 1/221 A23L 1/221 C 1/30 1/30 B A61K 7/00 A61K 7/00 K 7/06 7/06 7/075 7/075 7/08 7/08 7/48 7/48 7/50 7/50 // A23L 1/238 A23L 1/238 E

Claims (3)

[Claims] 1. One or more plants selected from the group consisting of almonds, dandelions, elderberry, sambucus japonicus, scutellaria japonica, soybean arbor, spinach, carrot, hop, mugwort, and yokunin,
A fibroblast proliferation promoter comprising an extract obtained by using one or a mixture of two or more of 1,3-butylene glycol, propylene glycol, and hexylene glycol. . 2. An external preparation for skin and a bath preparation, which comprises the fibroblast proliferation promoter according to claim 1. (3) A food or drink comprising the fibroblast proliferation promoter according to (1).