KR102121006B1 - Cosmetic composition for improvement of skin with Taraxacum officinale - Google Patents

Abstract

The present invention relates to a cosmetic composition for skin improvement containing a Taraxacum officinale leaf extract. The cosmetic composition of the present invention activates the proliferation of lactic acid bacteria in the skin to promote skin beneficial bacteria, and does not kill skin harmful bacteria but inhibits the same, thereby exhibiting an effect of controlling the growth of the skin beneficial bacteria and the skin harmful bacteria.

Description

{Cosmetic composition for improvement of skin with Taraxacum officinale}

The present invention relates to a cosmetic composition for skin improvement comprising an extract of Taraxacum officinale .

Microbiome is a compound word of a microbiota representing a microbial community and a genome representing a genome. Microbiota is a term that focuses on the microbial strain itself, compared to microbiome, and refers to a microorganism inhabiting the human body and having a symbiotic relationship. Interaction refers to research and technology based on genomics. However, recently, microbiota, microflora, and microbiome, which represent microorganisms, are commonly used. The term microbiome is better known as microbiome in the intestine, and inhibits harmful bacteria by ingesting lactic acid bacteria and expanding the power of lactic acid bacteria in the gut, and both lactic acid bacteria and harmful bacteria are microbiomes.

One of the reasons why microbiomes have attracted much attention in recent years is that the applicability of human health and disease problems has been greatly expanded. After the association of metabolic-related diseases such as obesity and diabetes in the body has been revealed, it has been found to be related to cancer, infection, immunity, and mental illness, and microbiome-based treatments are emerging as one of the innovative treatment technologies. Maintaining the balance of the microbiome is well known to affect the functioning of the human body, and interest in microbiome research is increasing as studies reveal that the microbiome in the body is associated with obesity.

The human microbiome market that is currently being formed is the functional food or wennis product market centered on probiotics and prebiotics, accounting for 83% of the total market. With the rapid growth of the health functional probiotics market, the demand for research and development in the fields of cosmetics, medicine and pharmaceuticals as well as food is rapidly increasing.

Recently, research on applying microbiome to the skin has been actively conducted. Known as harmful microorganisms on the skin Staphylococcus aureus (staphylococcus aureus) and propionic sludge bacterium when Solarium acne (Propionibacterium acnes) that increases as Staphylococcus known as beneficial microorganisms to the skin Caucus epidermidis (Staphylococcus epidermidis) the reduction of the skin Increased acne has been known for research. In order to prevent or restore this, many studies have been conducted in the cosmetics field, but most of them focus only on the antiseptic effect on harmful bacteria.

Korean Patent Registration No. 10-1376202 (2014.03.13) discloses a pharmaceutical composition for the prevention or treatment of skin diseases containing a mixture of dandelion and barley. Korean Patent Registration No. 10-1425757 (2014.07.25) discloses a skin massage composition for improving acne and a mask pack comprising the same.

The present invention is to develop and provide a new cosmetic composition having a skin microbiome efficacy using a prebiotic material that increases the growth of lactic acid bacteria in the skin.

The present invention provides a cosmetic composition for skin improvement comprising an extract of Taraxacum officinale .

In the cosmetic composition for skin improvement of the present invention, the skin improvement is preferably an improvement in acne skin and an improvement in skin oil/moisture balance.

The cosmetic composition for skin improvement of the present invention preferably promotes the growth of beneficial skin bacteria and suppresses the growth of harmful skin bacteria.

In the cosmetic composition for improving skin of the present invention, the beneficial bacteria are preferably Lactobacillus plantarum and Staphylococcus epidermidis .

In the cosmetic composition for improving skin of the present invention, the harmful bacteria are preferably Staphylococcus aureus and Propionibacterium acnes .

The cosmetic composition of the present invention promotes skin beneficial bacteria by activating the proliferation of lactic acid bacteria in the skin, and does not kill skin harmful bacteria, thereby suppressing the growth of skin beneficial bacteria and harmful bacteria.

1 is a result of evaluating the effect of controlling the skin lactic acid bacteria of Example 1 ( L. plantarum ), beneficial bacteria ( S. epidermidis ) and harmful bacteria ( S. aureus and P.acne ).
2 is a result of confirming a change in skin oil/moisture and pH of a subject during Example 1 treatment.
Figure 3 is the result of confirming the change in bacterial porphyrin during Example 1 treatment.
Figure 4 is the result of confirming the change in the distribution of skin microbes during Example 1 treatment (Bad: Staphylococcus aureus , Pseudomonas) aeruginosa , Corynebacterium jeikeium , Corynebacterium Diphtheriae , Streptococcus pyogenes, Streptococcus cristatus, Acinetobacter junii, Finegoldia magna, Stenotrophomonas maltophilia, Corynebacterium pseudogenitalium , P.acne: Propionibacterium acnes, Normal: P.Koreensis, Propionibacterium qranulosum, Staphyloccus, Staphylococcus , Acidovorax temperans, Dietzia maris, Bradyrhizobium japonicum , Acinetobater calcoaceticus , Good: Lactobacillus paracasei HS-05, Lactobacillus rhamnosus HK-9, Bacillus licheniformis, Bacillus subtilis ).

Probiotics function to control the composition of the microbiome, which also applies to the microbiome of the skin. A typical example of probiotics is lactic acid bacteria, and lactic acid bacteria use a substance called bacteriocin to suppress harmful bacteria. Existing studies have confirmed that certain lactic acid bacteria and metabolites are effective for skin diseases such as atopy.

However, the use of probiotics (live bacteria) as a cosmetic material exceeds the microbial limit, making it difficult to commercialize. Therefore, it was intended to develop a material having skin microbiome efficacy using a prebiotic material that increases the growth of lactic acid bacteria in the skin.

Accordingly, the present invention provides a cosmetic composition for improving skin comprising an extract of Western dandelion leaf ( Taraxacum officinale ).

In the case of materials containing sugars, which are well-known as nutrients for probiotics, there is a fear of formulation instability when applied to cosmetics in the future. Accordingly, the present applicant confirmed that the probiotic efficacy of the Western Dandelion leaf ( Taraxacum officinale ) is very excellent, and was selected as the material of the present invention.

In the cosmetic composition for skin improvement of the present invention, the skin improvement is preferably an improvement in acne skin and an improvement in skin oil/moisture balance. It was confirmed through the following experiments that the Western dandelion leaf extract of the present invention effectively improved the oil/moisture balance.

The cosmetic composition for skin improvement of the present invention preferably promotes the growth of beneficial skin bacteria and suppresses the growth of harmful skin bacteria. In this case, the beneficial bacteria are preferably Lactobacillus plantarum and Staphylococcus epidermidis , and the harmful bacteria are preferably Staphylococcus aureus and Prophy Propionibacterium acnes are good.

In the following experiment, the extract of the Western dandelion leaf of the present invention increases the growth of the skin beneficial bacteria Lactobacillus plantarum and Staphylococcus epidermidis , and Staphylococcus aureus, which is a skin harmful bacteria ( It was confirmed that Staphylococcus aureus ) and Propionibacterium acnes effectively reduced growth. In particular, the microbiome balance collapses when Staphylococcus epidermidis , a skin-beneficial bacterium, is killed by cosmetic preservatives, and the Western dandelion leaf extract of the present invention treats the concentration at which the preservative formulation is applied to the finished product. As a result of the experiment, it was confirmed that the growth of Staphylococcus epidermidis ( Staphylococcus epidermidis ) was increased (not shown), thereby solving the above-mentioned problem.

On the other hand, in the present invention, the extract is preferably contained 0.00001 to 30.0% by weight relative to the total weight of the cosmetic composition. More preferably, it is preferably contained 0.01 to 10.0% by weight relative to the total weight of the cosmetic composition. When the content of the mixed extract is less than 0.00001% by weight, there is no skin improvement effect including improvement of acne skin and improvement of skin oil/moisture balance, and when it exceeds 30.0% by weight, a marked effect of increasing content does not increase.

Meanwhile, the components included in the cosmetic composition of the present invention may include ingredients commonly used in cosmetic compositions in addition to the mixed extract of the present invention as an active ingredient, for example, antioxidants, stabilizers, solubilizers, vitamins, pigments and fragrances And conventional adjuvants, and carriers.

The cosmetic composition of the present invention can be prepared in any formulation conventionally prepared in the art, for example, solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing , Oil, powder foundation, emulsion foundation, wax foundation, pack, massage cream and spray, but may be formulated, but is not limited thereto. More specifically, it can be prepared in the form of flexible lotion, nutrition lotion, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder.

When the formulation of the cosmetic composition of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide, etc. This can be used.

When the formulation of the cosmetic composition of the present invention is a solution or an emulsion, a solvent, a solubilizing agent or an emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, and propylene Glycols, 1,3-butylglycol oil, glycerol aliphatic esters, polyethylene glycol or fatty acid esters of sorbitan.

When the formulation of the cosmetic composition of the present invention is a suspension, liquid diluents such as water, ethanol or propylene glycol as carrier components, suspensions such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester , Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or trakant, and the like.

When the formulation of the cosmetic composition of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in the case of a spray, additionally, chlorofluorohydro Propellants such as carbon, propane/butane or dimethyl ether.

When the formulation of the cosmetic composition of the present invention is a surfactant-containing cleansing, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivatives, methyltaurate, sarcosinate, as carrier components, Fatty acid amide ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

When the cosmetic composition of the present invention is a soap, a surfactant-containing cleansing formulation, or a surfactant-free cleansing formulation, it may be wiped, peeled off, or washed with water after being applied to the skin. As a specific example, the soap is liquid soap, powdered soap, solid soap and oil soap, and the surfactant-containing cleansing formulation is a cleansing foam, cleansing water, a cleansing towel and a cleansing pack, and the surfactant-free cleansing formulation is a cleansing cream , Cleansing lotion, cleansing water and cleansing gel, but are not limited thereto.

Hereinafter, the configuration of the present invention will be described in detail through the following examples and experimental examples. However, the scope of the present invention is not limited only to the following examples and experimental examples, and includes modifications to technical ideas equivalent thereto.

[ Experimental Example 1: Identification of skin lactic acid bacteria separation]

44 male and female subjects were swabbed by the surface, cheek, and palm skin microbes. After the swab swab was placed in a tube, the culture was carried out, and the culture was cultured in a BHI agar medium to obtain colonies. After culturing a plurality of single colonies in each BHI medium, sequencing analysis was commissioned to Macrogen Co., Ltd. to determine lactic acid bacteria for the culture strain.

To identify the isolated strain, the base sequence was analyzed using the 16S rRNA method, and it showed 99% homology with the standard strain, which was deposited with the Biological Resource Center and assigned accession number SD1323. The isolated lactic acid bacteria were named Lactobacillus plantarum RD-02, and were used in the following experiment to confirm the efficacy of the extract of the present invention on skin lactic acid bacteria.

[ Example 1: Preparation of western dandelion leaf extract of the present invention]

The dried dandelion leaf was pulverized into fine powders using a grinder. After mixing 50 g of Western dandelion leaf powder with 1 L of purified water, the mixture was stirred at 20-25° C. for 1 hour using a stirrer to prepare an extraction solution. The extraction solution was first filtered through a non-woven filter paper, and then the primary filtrate was no. Filtered under reduced pressure using 5C filter paper.

[ Experimental Example 2: Evaluation of skin microbial regulation efficacy]

In order to evaluate the efficacy of skin microbial control, the lactic acid bacteria'Lactobacillus plantarum RD-02' and Staphylococcus epidermidis ',' Lactobacillus plantarum RD-02' ( Lactobacillus plantarum RD-02) and Staphylococcus aureus ',' Lactobacillus plantarum RD-02) and Propionibacterium acnes at the same concentration The mixture was mixed with each and cultured together, and treated with the extract of Western dandelion leaf to incubate together. Thereafter, the culture medium was diluted and spread on agar medium, and then the colonies of each cultured microorganism were counted to confirm the change in the number of microorganisms.

As a result of the experiment, the growth of Staphylococcus epidermis, a beneficial bacterium of the skin, was significantly increased by activating the proliferation of Lactobacillus plantarum RD-02 (Accession No. SD1323), which is a skin lactic acid bacterium. It was confirmed that Staphylococcus aureus and Propionibacterium acnes significantly reduced growth (FIG. 1). 1 is a result of evaluating the effect of controlling the skin lactic acid bacteria of Example 1 ( L. plantarum ), beneficial bacteria ( S. epidermidis ) and harmful bacteria ( S. aureus and P.acne ).

[ Experimental Example 3: Skin safety evaluation]

In order to confirm skin safety, a human patch test was performed. 20 healthy adult women were treated with a diluted sample using the Finn chamber kit on the inner part of the arm and fixed to the skin. The criteria were based on the criteria of the International contact dermatitis research group. As a result of the experiment, it was confirmed that the extract of Western dandelion of the present invention had no irritation (Table 1).

Skin stability evaluation results sample Number of subjects Judgment result Irritation ++ + ± - Example 1 20 0 0 0 20 0 Control 20 0 0 0 20 0 (Criteria)
-: No erythema or unusual phenomena
±: slightly redder than the surroundings
+: Significantly reddening than the surroundings
++: Redden and swell more severely than the surroundings
(Calculation formula for stimulation)
Stimulus = [{(±)Number×1}+{(+)Number×2}+{(++)Number×3}/Number of Subjects

[ Experimental Example 4: Skin improvement efficacy evaluation]

When applied to actual skin, after confirming the improvement effect, after applying the sample of diluted Example 1 (concentration of dry weight 0.05%) to the skin, changes in the distribution of oil/moisture, pH, bacterial porphyrin and microorganisms in the skin Was confirmed.

For oil/moisture and pH measurements, a Soft Plus instrument from Callegari s.r.l was used, and an OBSERV instrument from Sylton diagnostic system was used for bacterial porphyrin measurements. Changes in skin microbes were analyzed by Seva Biotech.

As a result of the experiment, the oil/moisture level of the skin was improved to the level of normal skin according to the treatment of Example 1. When viewed as an average for all subjects, the moisture was improved by 10% and the oil content was improved by 20% (FIG. 2 ). 2 is a result of confirming a change in skin oil/moisture and pH of a subject during Example 1 treatment.

Bacteria porphyrin was reduced by about 25% when treated with Example 1. This figure was derived for the bacterial porphyrin at the tip of the nose, and it was confirmed that the Dot representing the bacterial porphyrin was also reduced at sites other than the tip of the nose (FIG. 3 ). Figure 3 is the result of confirming the change in bacterial porphyrin during Example 1 treatment.

On the other hand, as a result of confirming the microbial distribution change, in Example 1 treatment, the distribution of the bad type microbial community and the P. acne community decreased, and the normal type and good type microbial community distribution increased (FIG. 4 ). Figure 4 is the result of confirming the change in the distribution of skin microbes during Example 1 treatment (Bad: Staphylococcus aureus , Pseudomonas aeruginosa, Corynebacterium) jeikeium , Corynebacterium diphtheriae , Streptococcus pyogenes , Streptococcus cristatus , Acinetobacter junii , Finegoldia magna, Stenotrophomonas maltophilia , Corynebacterium pseudogenitalium , P.acne: Propionibacterium acnes , Normal: P. Koreensis , Propionibacterium qranulosum , Staphylococcus epidermidis , Streptococcus salivarius , Neisseria subflava, Staphylococcus warneri , Pseudomonas tolaaii , Acidovorax temperans , Dietzia maris , Bradyrhizobium japonicum , Acinetobater calcoaceticus , Good: Lactobacillus paracasei HS-05, Lactobacillus rhamnosus HK-9, Bacillus licheniformis, Bacillus subtilis ).

Claims (5)

Contains extract of Taraxacum officinale , activates the proliferation of skin lactic acid bacteria, promotes growth of beneficial skin bacteria and suppresses the growth of harmful skin bacteria,
The lactic acid bacteria is Lactobacillus plantarum ( Lactobacillus plantarum ),
The beneficial bacteria are Staphylococcus epidermidis ,
The harmful bacteria are Staphylococcus aureus ( Staphylococcus aureus ) and propionibacterium acne ( Propionibacterium acnes ) characterized in that the improvement of acne skin and skin oil / moisture balance cosmetic composition for improving. delete delete delete delete

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