KR102054911B1 - Composition for Improving Microbial Flora containing Amygdalin - Google Patents

Abstract

The present invention relates to a composition for improving microbial flora, comprising amygdalin as an active ingredient. The composition can inhibit the growth of harmful bacteria, and promote the growth of beneficial bacteria.

Description

Composition for improving microbial flora containing amidaline as an active ingredient {Composition for Improving Microbial Flora containing Amygdalin}

The present invention relates to a composition for improving the microbial flora comprising amidaline as an active ingredient.

The human body is an active ecosystem, and various microorganisms exist in the stomach and skin of the human body. The microbial community present in the stomach and skin is called a microbial flora, and 500 to 1000 or more different microorganisms symbiize and form a microbial flora. The intestinal microflora present in the stomach synthesizes vitamins and supplies it to the human body, and serves to suppress infection by pathogenic microorganisms introduced through contaminated food.

Microorganisms that live on the surface of the skin affect physiological activities such as human growth, nutrition, immunity, resistance to pathogenicity, etc., and block the invasion of external microorganisms, especially pathogenic microorganisms. In addition, microorganisms residing in the skin are known to perform the pH control and skin barrier function of the skin, change the skin lipid content to affect the skin condition. In patients with atopic dermatitis, the ceramide content of the stratum corneum is reduced, resulting in dry, cracked keratinous skin. In these atopy patients, the skin flora is known to be extremely staphylococcus aureus (Non-Patent Document 1). ).

Probiotics is a compound word of pro and biotics, and the World Health Organization (WHO) defines it as a living microorganism that can have a beneficial effect on health when consumed in sufficient quantities. Most probiotics known to date are lactic acid bacteria, which have beneficial effects such as breaking down foods in the intestines and synthesizing vitamins after reaching the intestines.

Prebiotics are non-digestive nutrients that help the growth and activity of probiotics. They are known to have a beneficial effect on health by increasing the proportion of probiotics in the microbial flora. Prebiotics are mostly dietary fiber, and raffinose, soy oligosaccharide, lactitol, xylitol and the like are known.

Since microorganisms also have a beneficial effect on the human body, it is necessary not only to remove (sterilize) microorganisms, but also to promote the growth of beneficial bacteria while suppressing the growth of harmful bacteria. The present inventors completed the present invention by performing a study for improving the microbial flora using natural products.

 HC Korting et al., Eur. J. Clin. Pharmacol. 39 (1), 33-36. 1990.

One object of the present invention to provide a composition for improving microbial flora comprising amidaline as an active ingredient.

One aspect of the present invention to achieve the above object provides a composition for improving the microbial flora comprising amidaline as an active ingredient.

As used herein, the term "microbial flora" refers to a microbial community including all bacteria, fungi and other prokaryotes present in the human body, and mainly lives in the stomach and skin. For example, intestinal yuikgyun that live in the stomach has a Lactobacillus bacteria (Lactobacillus), Bifidobacterium (Bifidobacterium), harmful bacteria in the intestines is Clostridium (Clostridium) are known. The beneficial bacteria inhabiting the skin include Staphylococcus epidermidis (epidermal staphylococcus) and the harmful bacteria include Staphylococcus aureus ( Staphylococcus aureus ).

As used herein, the term 'microbial flora improvement' refers to a state in which the composition of the microbial flora is changed in a direction in which the specific gravity of harmful bacteria decreases and the specific gravity of beneficial bacteria increases due to the administration of the composition.

Amigdaline, which is used as an active ingredient of the composition for improving microbial flora of the present invention, is a compound having the structure of Formula (I). It is found in apricot and peach seeds, also called vitamin B ₁₇ or laetrile. Amigdalin releases hydrogen cyanide (HCN) by beta-glucosidase, which is present only in cancer cells in large amounts, and is known to have anticancer effects.

[Formula I]

In one embodiment of the present invention, the amidaline may be used both from natural products, synthesized by organic chemical methods known in the art or commercially available.

In one embodiment of the present invention, the composition for improving the microbial flora can comprise amidAGline 0.005 to 10% by weight, or 1 to 5% by weight based on the total weight of the composition. In addition, the composition may include amigdaline from 0.005 mM to 50 mM, or 0.5 mM to 5 mM, and the content of amigdaline may be appropriately adjusted according to the method of use and purpose of use of the composition.

In one embodiment of the present invention, the composition for improving the microbial flora can promote the growth of beneficial bacteria while inhibiting the growth of harmful bacteria. Increasing the proportion of beneficial bacteria in the microbial flora can prevent infection by pathogenic microorganisms and improve immunity.

In one embodiment of the invention, the harmful bacteria is a propionyl sludge tumefaciens (Propionibacterium), Streptococcus (Streptococcus), Candida (Candida), Acinetobacter (Acinetobacter), Corynebacterium (Corynebacterium) and Pseudomonas (Pseudomons) It may be one or more selected from the group consisting of, but is not limited thereto.

In one embodiment of the present invention, the harmful bacterium may be Staphylococcus aureus . Staphylococcus aureus is a gram-positive, communicable anaerobic bacterium that is present on the skin and nasal surfaces of humans and livestock. Staphylococcus aureus is known as a causative agent of not only food poisoning but also purulent diseases such as purulent skin, otitis media and cystitis.

In one embodiment of the present invention, the beneficial bacteria may be Staphylococcus epidermidis . Staphylococcus epidermidis is a gram-positive bacterium that is commonly found in human and livestock skin and mucous membranes. Staphylococcus epidermidis produces glycerin to sustain the moisturizing effect of the skin (J. Invest. Dermatol., 1960, 34: 171-174), and synthesizes organic acids to keep the skin acidic, which has a beneficial effect on the skin (Br. J. Dermatol. 1986, 80: 279-281).

In one embodiment of the present invention, the composition for improving the microbial flora may be a cosmetic composition for improving the skin flora.

As used herein, the term 'skin microbial flora' refers to a microbial community existing on the skin, and mainly inhabits the skin surface and pores such as the scalp. The skin flora contains skin beneficial bacteria that have a beneficial effect on the skin, such as maintaining skin moisturizing, removing free radicals, and skin harmful bacteria causing skin problems such as acne and purulent.

In one embodiment of the present invention, the skin benefit bacteria may be Staphylococcus epidermidis, and the skin harmful bacteria may be Staphylococcus aureus.

In one embodiment of the present invention, the cosmetic composition is a gel, emulsion, suspension, microemulsion, microcapsules, microgranules, ionic vesicle dispersant, nonionic vesicle dispersant, cream, skin, lotion, powder, ointment, spray, It may be formulated in the form of a cone seal stick, aerosol and mask pack. Examples of the cosmetic composition may include a basic cosmetic, a makeup cosmetic, a hair cosmetic, a body cosmetic, and the like, and may be appropriately selected according to the purpose.

For example, the cosmetic composition is formulated into solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations, sprays, and the like. It may be, but is not limited thereto. More specifically, softening cream, nourishing cream, lotion, body lotion, nourishing cream, massage cream, moisturizing cream, hand cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, gel, patch, oil in water basic cosmetics such as (oil-in-water, O / W) and water-in-oil (W / O), color cosmetics such as lipstick, makeup base or foundation, shampoo, rinse, body cleanser, It may be formulated into a cosmetic composition for hair, such as cleaning agents such as toothpaste or mouthwash, hair fixatives such as hair tonic, gel or mousse, wool or hair dye.

In one embodiment of the invention, when the formulation of the cosmetic composition is a solution or emulsion, a solvent, solubilizer or emulsifier is used as the carrier component, for example water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol , Fatty acid esters of benzyl benzoate, propylene glycol, 1,3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

In one embodiment of the invention, when the formulation of the cosmetic composition is a suspension, a liquid diluent such as water, ethanol or propylene glycol, ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbent as carrier components Suspending agents such as non-elastic esters, microcrystalline cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

In one embodiment of the present invention, when the formulation of the cosmetic composition is a paste, cream or gel as a carrier component, animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica , Talc or zinc oxide may be used.

In one embodiment of the present invention, when the formulation of the cosmetic composition is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, particularly in the case of a spray May additionally include propellants such as chlorofluorohydrocarbons, propane / butane or dimethyl ether.

In addition, in the cosmetic composition of each formulation, other components other than amigdaline may be arbitrarily selected and blended according to the formulation or purpose of use of the cosmetic composition. In addition, the compositions of each formulation may contain various bases and additives necessary for the formulation of the formulation, and nonionic surfactants, silicone polymers, extender pigments, fragrances, and preservatives within a range that does not reduce the effect. , Fungicides, oxidation stabilizers, organic solvents, ionic or nonionic thickeners, softeners, antioxidants, free radical destroyers, opacifiers, stabilizers, emolients, silicones, α-hydroxy acids acid), antifoaming agents, humectants, vitamins, preservatives, surfactants, fillers, polymers, propellants, basicizing agents, acidifying agents, or coloring agents.

In one embodiment of the present invention, the composition for improving the microbial flora can be used as a cleaning composition. When used as a cleaning composition, the specific gravity of harmful bacteria is reduced, and the specific gravity of beneficial bacteria is increased, thereby improving skin condition after washing and improving body odor.

In one embodiment of the present invention, the detergent composition may be selected from the group consisting of shampoo, rinse, hair conditioner, body wash, soap, hand wash, laundry detergent and kitchen detergent.

Amigdalin promotes the growth of beneficial bacteria while inhibiting the growth of harmful bacteria, and thus can be usefully used for improving microbial flora and skin flora.

Figure 1 shows the results of confirming the growth change by the amidaline treatment in the mixed culture of Staphylococcus epidermidis and Staphylococcus aureus .

Hereinafter, one or more embodiments will be described in more detail with reference to Examples. However, these examples are provided to illustrate one or more embodiments illustratively and the scope of the present invention is not limited to these examples.

Example  One: Amigdaline  Growth of Skin-resident Microorganisms

Among the resident microorganisms, Staphylococcus aureus ( Staphylococcus aureus : Staphylococcus aureus: known as S. aureus ), which is known as a causative agent of purulent diseases such as purulent otitis media, otitis media and cystitis, was used. As a beneficial bacterium , Staphylococcus epidermidis ( E. epidermal staphylococci: hereinafter referred to as S. epidermidis ) was used to synthesize glycerin, organic acids and the like, which have a beneficial effect on the skin.

1-1. Microbial culture

S. aureus used in the experiment KCTC 1916 and S. epidermidis KCTC 1917 strains were purchased from the Korean Collection for Type Cultures (South Korea). The medium used for the culture of the microorganisms is shown in Table 1.

S. epidermidis
KCTC 1917 NB
(100 ml) Nutrient Broth 0.8 g (Agar) 1.5 g water Replenish to a total volume of 100 ml S. aureus
KCTC 1916 TSB
(100 ml) Tryptic Soy Broth 3.0 g (Agar) 1.5 g water Replenish to a total volume of 100 ml

1-2. Growth Changes of Skin-resident Microorganisms by Amidaline

S. aureus KCTC 1916 strain stock was plated on TSB (Becton Dickinson and Company, USA) agar plates and incubated at 37 ° C. for 24 hours. Thereafter, a single colony was taken and inoculated in 5 ml of TSB and pre-cultured overnight at 37 ° C. and 250 rpm.

S. epidermidis KCTC 1917 strains were precultured in the same manner as above, except that the stock was plated on a Becton Dickinson and Company (AB) agar plate.

Precultured S. aureus KCTC 1916 strain and S. epidermidis KCTC 1917 strain were mixed and inoculated in 20 ml of NB to make OD ₆₀₀ 0.05. D-amygdalin hydrate (96%: Alfa Aesar, Heysham, England) dissolved in methanol was added at 0.25, 0.5, 1, 2.5 and 5 mM concentrations, respectively, and the control group contained methanol in 1% (v / v) was added at a concentration and incubated for 3 hours at 37 ℃, 250 rpm. Subsequently, 100 μl of the culture solution was plated on an NB agar plate, and cultured at 37 ° C. for 24 hours to determine CFU (Colony forming unit).

As a result, as shown in FIG. 1, the growth of S. epidermidis was increased and the growth of S. aureus was markedly decreased by amigdaline treatment.

Those skilled in the art will appreciate that the present invention can be implemented in a modified form without departing from the essential features of the present invention. Therefore, the disclosed embodiments should be considered in descriptive sense only and not for purposes of limitation. The scope of the present invention is shown in the claims rather than the foregoing description, and all differences within the scope will be construed as being included in the present invention.

Claims (8)

As a composition for improving the skin microbial flora comprising amigdalin (amygdalin) as an active ingredient,
The skin microbial flora includes Staphylococcus aureus and Staphylococcus epidermidis ,
The composition is to inhibit the growth of Staphylococcus aureus, to promote the growth of Staphylococcus epidermidis to improve the skin microbial flora.
delete delete delete The composition of claim 1, wherein the composition is a cosmetic composition for skin improvement.
The method of claim 5, wherein the cosmetic composition is a gel, emulsion, suspension, microemulsion, microcapsules, microgranules, ionic vesicle dispersant, nonionic vesicle dispersant, cream, skin, lotion, powder, spray, conceal stick, aerosol And a mask pack formulation.
The composition of claim 1, wherein the composition is a detergent composition.
8. The composition of claim 7, wherein the detergent composition is selected from the group consisting of shampoos, rinses, hair conditioners, body washes, soaps, hand washes, laundry detergents and kitchen detergents.

Images