KR102031360B1 - Composition for improving microbial flora containing extract of violae herba - Google Patents

Abstract

Disclosed is a composition for improving the microbial flora, including the designated magnetization extract as an active ingredient, the composition can inhibit the growth of harmful bacteria, and promote the growth of beneficial bacteria.

Description

Composition for improving microbial flora containing extract of violae herba}

The present invention relates to a composition for improving microbial flora comprising a designated magnetized extract as an active ingredient.

The human body is a complex and active ecosystem, and various microorganisms exist in the stomach and skin of the human body. The microbial community present in the stomach and skin is called a microbial flora, and 500 to 1000 or more different microorganisms symbiize and form a microbial flora. The intestinal microflora present in the stomach synthesizes vitamins and supplies it to the human body, and serves to suppress infection by pathogenic microorganisms introduced through contaminated food.

Microorganisms that live on the surface of the skin affect physiological activities such as human growth, nutrition, immunity, resistance to pathogenicity, etc., and block the invasion of external microorganisms, especially pathogenic microorganisms. In addition, microorganisms residing in the skin are known to perform the pH control and skin barrier function of the skin, change the skin lipid content to affect the skin condition. For example, in the case of atopic patients it has a horny skin, dry the ceramide content of the horny layer is reduced, and cracks in these atopic patients known to be changed by the skin microflora is extremely Staphylococcus aureus (Staphylococcus aureus) (Non-Patent Document One).

Since microorganisms have microorganisms that have a beneficial effect on the human body, it is necessary not only to remove (sterilize) all the microorganisms, but also to promote the growth of beneficial bacteria while suppressing the growth of harmful bacteria.

The present inventors have completed the present invention as a result of performing research for improving the microbial flora using natural products.

 HC Korting et al., Eur J Clin Pharmacol 39 (1), 33-36. 1990.

It is an object of the present invention to provide a composition for improving microbial flora and skin cosmetic composition for improving the flora of the present invention, including a designated extract as an active ingredient.

One aspect of the present invention to achieve the above object provides a composition for improving microbial flora comprising a magnetization designated extract as an active ingredient.

As used herein, the term "microbial flora" refers to a microbial community including all bacteria, fungi and other prokaryotes present in the human body, and mainly lives in the stomach and skin. For example, intestinal yuikgyun that live in the stomach has a Lactobacillus bacteria (Lactobacillus), Bifidobacterium (Bifidobacterium), harmful bacteria in the intestines is Clostridium (Clostridium) are known.

As used herein, the term 'improvement' means a state in which the composition of the microbial flora is changed in a direction beneficial to the human body due to the administration of the composition.

Violae herba used as an active ingredient of the composition for improving microbial flora of the present invention refers to medicinal herbs made of outposts of Violet Viola mandshurica Baker or Violet Viola yedoensis Makino (Violet and Violaceae), and detoxification by pharmacological action, Antibacterial action, anti-inflammatory action is known. However, the efficacy of microbial flora improvement is not known.

In one embodiment of the present invention, the magnetization directed extract may be prepared according to conventional methods known in the art. For example, the magnetization designation extract may be prepared by pulverizing the magnetization designation, adding a solvent commonly used for extraction, and extracting at an appropriate temperature and pressure.

In one embodiment of the invention, the solvent is selected from the group consisting of water, hexane, 1,3-butylene glycol, alcohol having 1 to 4 carbon atoms, ethyl acetate, diethyl ether, dichloromethane, acetone and mixtures thereof Can be.

In one embodiment of the present invention, the solvent may be an alcohol having 1 to 4 carbon atoms, methanol or ethanol may be used.

On the other hand, the magnetization designated extract includes both the extract by the solvent extraction method and the extract that has undergone a conventional purification process. For example, separation using an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity), etc. Fractions obtained through various purification methods may also be included in the directed magnetized extract of the present invention. In addition, the magnetization designated extract may be prepared in a powder state by an additional process such as distillation under reduced pressure and freeze drying or spray drying.

In one embodiment of the present invention, the composition for improving the microbial flora can include 0.005 to 10% by weight, or 1 to 5% by weight of the magnetization specified extract based on the total weight of the composition, the method of use and purpose of use of the composition According to the content of the active ingredient can be adjusted appropriately.

In one embodiment of the present invention, the composition for improving the microbial flora can promote the growth of beneficial bacteria while inhibiting the growth of harmful bacteria. Increasing the proportion of beneficial bacteria in the microbial flora can prevent infection by pathogenic microorganisms, improve immunity, improve skin condition and improve body odor.

In one embodiment of the invention, the harmful bacteria is Propionibacterium sludge tumefaciens (Propionibacterium), Staphylococcus (Staphylococcus), Streptococcus (Streptococcus), Candida (Candida), Acinetobacter (Acinetobacter), Corynebacterium (Corynebacterium ) and be at least one selected from the group consisting of Pseudomonas (Pseudomons), but it is not limited thereto. In addition, the harmful bacterium may be Propionibacterium acnes or Candida albicans .

In one embodiment of the present invention, the harmful bacterium may be Staphylococcus aureus ( Staphylococcus aureus ). Staphylococcus aureus is a gram-positive, communicable anaerobic bacterium that is commonly present on the skin and nasal surfaces of healthy humans and livestock. S. aureus is known to cause foodborne pathogens as well as maturation, ear infections, bladder infections such as purulent diseases of the skin.

In one embodiment of the present invention, the beneficial bacteria may be Staphylococcus epidermidis ( S. epidermidis ). Staphylococcus epidermidis is a gram-positive bacterium that is commonly found in human and livestock skin and mucous membranes. Staphylococcus epidermidis produces glycerin to sustain the moisturizing effect of the skin (J. Invest. Dermatol., 1960, 34: 171-174), synthesizes organic acids to keep the skin acidic (Br. J. Dermatol) 1986, 80: 279-281), producing antibiotics that are known to inhibit skin growth of harmful microorganisms such as Staphylococcus aureus (N. Engl. J. Med., 1998, 339: 520-). 532; Nature, 2010, 465: 346-349; Peptides, 2010, 31: 1661-1668; J. Invest.Dermatol., 2010, 130: 192-200).

Another aspect of the present invention provides a cosmetic composition for improving skin flora comprising a designated magnetized extract as an active ingredient.

As used herein, the term 'skin residential flora' refers to a microbial community existing on the skin, and mainly inhabits the skin surface and pores such as the scalp. The skin flora contains skin beneficial bacteria that have a beneficial effect on the skin, such as maintaining skin moisturizing, removing free radicals, and skin harmful bacteria causing skin problems such as acne and purulent.

In one embodiment of the present invention, the cosmetic composition is the cosmetic composition is a gel, emulsion, suspension, microemulsion, microcapsules, microgranulocytes, ionic vesicle dispersant, nonionic vesicle dispersant, cream, skin, lotion, powder, It may be formulated in the form of ointments, sprays, conceal sticks, aerosols, mask packs, body powders, body washes, shampoos, hair conditioners, body lotions, body pacts for deodorants and body mists. Examples of the cosmetic composition may include a basic cosmetic, a makeup cosmetic, a hair cosmetic, a body cosmetic, and the like, and may be appropriately selected according to the purpose.

For example, the cosmetic composition is formulated into solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations, sprays, and the like. It may be, but is not limited thereto. More specifically, softening cream, nourishing cream, lotion, body lotion, nourishing cream, massage cream, moisturizing cream, hand cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, gel, patch, oil in water basic cosmetics such as (oil-in-water, O / W) and water-in-oil (W / O), color cosmetics such as lipstick, makeup base or foundation, shampoo, rinse, body cleanser, It may be formulated as a cosmetic composition for hair, such as cleaning agents such as toothpaste or mouthwash, hair fixatives such as hair tonic, gel or mousse, wool or hair dye.

In one embodiment of the invention, when the formulation of the cosmetic composition is a solution or emulsion, a solvent, solubilizer or emulsifier is used as the carrier component, for example water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol , Fatty acid esters of benzyl benzoate, propylene glycol, 1,3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

In one embodiment of the invention, when the formulation of the cosmetic composition is a suspension, a liquid diluent such as water, ethanol or propylene glycol, ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbent as carrier components Suspending agents such as non-elastic esters, microcrystalline cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

In one embodiment of the present invention, when the formulation of the cosmetic composition is a paste, cream or gel as a carrier component, animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica , Talc or zinc oxide may be used.

In one embodiment of the present invention, when the formulation of the cosmetic composition is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as the carrier component, particularly in the case of spray May additionally include propellants such as chlorofluorohydrocarbons, propane / butane or dimethyl ether.

In addition, in the cosmetic composition of each formulation, other components other than the designated magnetization extract may be arbitrarily selected and blended according to the formulation or purpose of use of the cosmetic composition. In addition, the compositions of each formulation may contain various bases and additives necessary for the formulation of the formulation, and nonionic surfactants, silicone polymers, extender pigments, fragrances, and preservatives within a range that does not reduce the effect. , Fungicides, oxidation stabilizers, organic solvents, ionic or nonionic thickeners, softeners, antioxidants, free radical destroyers, opacifiers, stabilizers, emolients, silicones, α-hydroxy acids acid), antifoaming agents, humectants, vitamins, preservatives, surfactants, fillers, polymers, propellants, basicizing agents, acidifying agents, or coloring agents.

Self-designated extract can be useful for microbial flora improvement and skin flora improvement because it inhibits the growth of harmful bacteria and promotes the growth of beneficial bacteria.

Figure 1 shows the results of measuring the growth rate changes of Staphylococcus epidermidis and Staphylococcus aureus by the treatment of the designated magnetized extract.
Figure 2 shows the growth change by the magnetized directed methanol extract treatment when the mixed culture of Staphylococcus epidermidis and Staphylococcus aureus.
Figure 3 shows the change in growth by treatment with a designated 95% ethanol extract of Staphylococcus aureus and Staphylococcus epidermidis.

Hereinafter, one or more embodiments will be described in more detail with reference to Examples. However, these examples are provided to illustrate one or more embodiments illustratively and the scope of the present invention is not limited to these examples.

Example 1 Preparation of Magnetized Specified Extract

50 g of 95% ethanol or methanol (medicinal: solvent ratio 1:10) was added to 5 g of magnetization, and shaken for 3 hours in a 50-temperature water bath to obtain a primary extract. The primary extract was filtered through filter paper, and the filtered extract was concentrated and dried to prepare a magnetized extract. 50 mg of the dried magnetized designated extract was dissolved in 1 ml of each extraction solvent, and the solids not dissolved in the solvent were removed by centrifugation, and only the supernatant was used as a sample.

Example 2 Growth of Skin Microorganisms due to Specified Magnetization Extract

2-1. Microbe and Culture Media

Staphylococcus aureus ( Staphylococcus aureus ; hereinafter referred to as S. aureus ) is used as a skin harmful bacterium, and Staphylococcus epidermidis (Sermatosclerosis; hereinafter S. aureus ) is used as a skin beneficial bacterium . epidermidis ).

S. aureus KCTC 1916 and S. epidermidis KCTC 1917 used in the experiment were purchased from the Korean Collection for Type Cultures (Korea). The medium composition used for the culture of the two microorganisms is shown in Table 1 below.

S. epidermidis
KCTC 1917 NB Badge
(100 ml) Nutrient Broth 0.8 g (Agar) 1.5 g water Replenish to a total volume of 100 ml S. aureus
KCTC 1916 TSB badge
(100 ml) Tryptic Soy Broth 3.0 g (Agar) 1.5 g water Replenish to a total volume of 100 ml

2-2. Growth of Individual Skin-Resident Microorganisms Caused by Magnetized Extract

S. aureus KCTC 1916 strain stock was plated on TSB (Tryptic Soy Broth, Becton Dickinson and Company, USA) agar plates and incubated at 37 ° C. for 24 hours. A single colony was then inoculated in 5 ml of TSB liquid medium and preincubated overnight at 37 ° C. and 250 rpm. Precultured S. aureus KCTC 1916 strain was inoculated in 20 ml of TSB liquid medium such that OD ₆₀₀ was 0.05, and 200 µl (1% (v / v)) of magnetized methanol extract was added. In the control group, methanol was added at a concentration of 1% (v / v) instead of the designated magnetization extract. Subsequently, the degree of growth was confirmed by measuring absorbance at 600 nm using an Optizen 2120 UV plus spectrophotometer (Mecasys Co., Ltd., Daejeon, Korea) every 1 hour while incubating at 37 ° C. and 250 rpm.

S. epidermidis KCTC 1917 In addition, the stock was cultured in the same manner as above, except that the stock was grown on NB (nutrients broth, Becton Dickinson and Company) agar plates, and the growth was measured.

As a result, as shown in FIG. 1, the growth rate of S. epidermidis was increased and the growth rate of S. aureus was decreased by the designated methanol extract. Specifically, the doubling time of S. epidermidis was reduced by about 33.51% in the magnetized extract extract treatment group (51.34 minutes) compared to the control group (77.22 minutes). On the other hand, the doubling time of S. aureus was increased by 43.65% in the magnetized extract extract treatment group (50.45 minutes) compared to the control group (35.12 minutes).

2-3. Growth Changes of Skin-resident Microorganisms by Magnetized Methanol Extract

Microorganisms were precultured in the same manner as in Example 2-2, and the precultured S. aureus KCTC 1916 strain and S. epidermidis KCTC 1917 strain were mixed and inoculated in 20 ml of NB liquid medium such that OD ₆₀₀ was 0.05. Magnetized directed methanol extract was added to 200 μl (1% (v / v)), and the control group was incubated at 37 ° C., 250 rpm for 3 hours by adding methanol at a concentration of 1% (v / v). Thereafter, 100 μl of the culture solution was plated on an NB agar plate, and cultured at 37 ° C. for 24 hours to confirm CFU (Colony forming unit).

As a result, as shown in FIG. 2, the growth of S. epidermidis was increased and the growth of S. aureus was significantly decreased by the magnetization-designated methanol extract treatment.

2-4. Growth Changes of Skin-resident Microorganisms by 95% Ethanol Extract

Except for treating the 95% ethanol extract of the magnetization directed at different concentrations (0 to 0.5 g / L), the growth of the microorganism was confirmed in the same manner as in the 2-3.

As a result, as shown in FIG. 3, the growth of S. aureus was remarkably decreased by the treatment of 95% ethanol extract designated by magnetization, and the growth of S. epidermidis was remarkably increased.

Those skilled in the art will appreciate that the present invention can be implemented in a modified form without departing from the essential features of the present invention. Therefore, the disclosed embodiments should be considered in descriptive sense only and not for purposes of limitation. The scope of the present invention is shown in the claims rather than the foregoing description, and all differences within the scope will be construed as being included in the present invention.

Claims (5)

In the cosmetic composition for skin microbial flora improvement to inhibit the growth of harmful bacteria containing a designated magnetized extract as an active ingredient, promoting the growth of beneficial bacteria,
The harmful bacterium is Staphylococcus aureus ,
The beneficial bacteria is Staphylococcus epidermidis ,
Extraction solvent of the designated magnetized extract is ethanol aqueous solution or methanol,
The ethanol aqueous solution is a mixture of ethanol and water in a volume ratio of 95: 5
Cosmetic composition for improving skin microbial flora.
delete The composition of claim 1, wherein the magnetized extract is included in an amount of 0.005 to 10% by weight based on the total weight of the composition.
delete delete

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