KR101951646B1 - A cosmetic composition comprising marinobacter-fermented spirulina extract for antioxydant and skin winkle improvement - Google Patents

Abstract

The present invention provides a cosmetic composition for antioxidation and wrinkle amelioration, including a spirulina marinobacter fermented extract as an active ingredient.

Description

TECHNICAL FIELD [0001] The present invention relates to a cosmetic composition for improving antioxidative and anti-wrinkle properties, comprising an extract of Ferulina marinobacter fermented extract as an active ingredient,

The present invention relates to a cosmetic composition comprising a fermented extract of Spirulina marinobacter as an active ingredient.

Skin is the most basic defensive body that protects the body against irritation in contact with the external environment, and is an important body expressing external beauty.

Since external beauty is not only a standard of judgment that symbolizes youth and health but also a competitiveness that affects business ability by expressing self-confidence, the tendency to manage skin in modern society is increasing.

However, as the age increases, the secretion of various hormones that regulate metabolism decreases, the function of immune cells and the activity of cells decrease, and the biosynthesis of immune proteins and biocompatible proteins necessary for the living body is decreased, and the ozone layer destruction , The free radicals and free radicals caused by environmental pollution such as physical and chemical stimuli and stress caused by the increase in the normal function of the skin is weakened to promote aging, the color is bad and the skin is wrinkled.

In order to prevent such phenomenon and to maintain the skin with a bright tone, physiologically active substances obtained from various known animals, plants and microorganisms were added to cosmetics to prevent skin aging caused by oxidation, thereby trying to improve the skin.

The cosmetics industry is developing a number of products using natural products to reduce skin irritation caused by various chemicals. In addition to low adverse effects on skin, natural materials have recently been increasingly developed as cosmetic raw materials, as consumers have become more receptive to cosmetics using natural materials.

However, a cosmetic product containing a natural-derived extract obtained by an ordinary method has not been sufficiently functionalized, such as skin improving effect, and the skin improving activity is not maintained constantly.

Accordingly, there is a continuing need to develop cosmetic products derived from natural products, which have excellent functionalities and excellent biostability and skin improving effect.

Recently, Spirulina has been attracting attention as a functional cosmetic material. Spirulina is a blue-green cyanobacterium that grows wild in the tropics while photosynthesising on the surface of a high-alkaline, salty lake. Spirulina has been attracting attention as a health functional food because it has a high concentration of proteins and various nutrients.

In particular, it has been officially recognized by the United Nations, the World Health Organization and the Food and Drug Administration (FDA) as a complete food product in terms of safety and nutrition as a health food. It is also a decided food.

Spirulina is a high concentration of all the nutrients that are needed by the organism, especially the proteins and the natural chlorophyll nutrients of the greenish-yellow vegetables. It contains five nutrients (protein, carbohydrate, various minerals, fat, various vitamins) necessary for the human body, and even nutrients from 20,000 to 30,000 kinds.

In addition, it has a very high protein content (55 ~ 77%) and various components are composed almost same ratio with the human body, so digestion rate of body is more than 95%, and the digestive function is weakened, the elderly, It is a strong alkaline whole food that does not cause imbalance in nutrition even when used for a long time.

Recently, anticancer effect and various immune function enhancing effects have been reported, and it can be developed as a functional cosmetic material because it contains a large amount of various vitamins. However, the use of Spirulina as a functional cosmetic material has not yet been specifically identified for skin physiological activity and skin efficacy.

On the other hand, it is called fermentation that a substance useful for living is produced in the process of decomposing organic matter using an enzyme possessed by a microorganism. Natural products include physiologically active ingredients such as various amino acids, organic acids, and natural antioxidants that are beneficial to skin. It is known that fermentation of natural products through useful microorganisms increases the biologically active component twice before fermentation.

Various cosmetics industry has continued to develop natural cosmetics with enhanced functionality through such fermentation process. Particularly, the fermented natural product increases the skin absorption rate because particles of various functional ingredients are reduced through the fermentation process. In addition, since a new physiologically active ingredient which has not existed can be produced, various functional effects can be exhibited by fermenting a natural product.

Therefore, the present inventors intend to develop a cosmetic composition having improved functionality by using spirulina.

According to one aspect of the present invention, there is provided a cosmetic composition comprising, as an active ingredient, a spirulina fermented extract obtained by inoculating Marinobacter shengliensis .

In one embodiment, the fermentation extract may be obtained by fermenting the fermentation extract at 23 to 27 ° C.

In one embodiment, the fermentation extract may be obtained by fermenting the fermentation extract for 96 to 144 hours.

In one embodiment, the cosmetic composition may be for antioxidant or wrinkle improvement.

In one embodiment, the cosmetic composition is selected from the group consisting of a skin, a lotion, a cream, a serum, an emulsion, an essence, a powder, a foundation, a spray, a mask pack, a sheet pack, a sleep pack, Or more.

The cosmetic composition according to one aspect of the present invention is excellent in antioxidative and wrinkle-reducing effects because it contains a ferulic extract of Spirulina as an active ingredient.

It should be understood that the effects of the present invention are not limited to the effects described above, but include all effects that can be deduced from the description of the invention or the composition of the invention set forth in the claims.

FIG. 1 is a graph illustrating the antioxidative activity of fermented extract according to an embodiment of the present invention.

FIG. 2 is a graph showing the results of measurement of collagenase inhibitory activity of the fermented extract according to an embodiment of the present invention.

FIG. 1 is a graph illustrating the antioxidative activity of fermented extract according to an embodiment of the present invention.
FIG. 2 is a graph showing the results of measurement of collagenase inhibitory activity of the fermented extract according to an embodiment of the present invention.

As used herein, the terminology used herein is intended to encompass all commonly used generic terms that may be considered while considering the functionality of the present invention, but this may vary depending upon the intent or circumstance of the skilled artisan, the emergence of new technology, and the like. Also, in certain cases, there may be a term selected arbitrarily by the applicant, in which case the meaning thereof will be described in detail in the description of the corresponding invention. Therefore, the term used in the present invention should be defined based on the meaning of the term, not on the name of a simple term, but on the entire contents of the present invention.

Unless defined otherwise, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Terms such as those defined in commonly used dictionaries are to be interpreted as having a meaning consistent with the contextual meaning of the related art and are to be interpreted as either ideal or overly formal in the sense of the present application Do not.

The numerical range includes numerical values defined in the above range. All numerical limitations of all the maximum numerical values given throughout this specification include all lower numerical limitations as the lower numerical limitations are explicitly stated. All the minimum numerical limitations given throughout this specification include all higher numerical limitations as the higher numerical limitations are explicitly stated. All numerical limitations given throughout this specification will include any better numerical range within a broader numerical range, as narrower numerical limitations are explicitly stated.

Hereinafter, embodiments of the present invention will be described in detail, but it should be apparent that the present invention is not limited by the following examples.

According to one aspect of the present invention, there is provided a cosmetic composition comprising, as an active ingredient, a spirulina fermented extract obtained by inoculating a fermentation strain.

The cosmetic composition contains Spirulina fermented extract as an active ingredient and has excellent antioxidative and wrinkle-reducing effects, and also has little skin irritation.

The fermentation strain may be Marinobacter , and preferably Marinobacter shengliensis .

The Marinobacter senrygensis is a gram-negative bacterium. It is a horny, aerobic bacterium having a single polar flagellum and can be isolated from the lava seawater of Jeju Island.

The inventors of the present invention have confirmed that the hydrolysis of the active ingredient contained in Spirulina is promoted by the fermentation of Marinobacterium gingensis to thereby increase the diverse skin components and thus the skin improving effect such as anti-stress and anti-inflammatory activity can be remarkably improved .

As used herein, the term " comprising as an active ingredient " means an effective amount that can exhibit a skin improving effect, for example, a degree of collagen synthesis associated with a wrinkle reducing effect, an elasticity improvement, a skin irritation alleviation, can do.

The "Spirulina" is the oldest algae on Earth and is a multi-celled organism with thin walls. The spirulina is naturally grown in a highly salted alkaline environment, and can be mass-produced in an artificial form. The spirulina is spiral in a cyan green color and has a length of 300 to 500 탆 and a width of 8 탆. Approximately 30 species are known and consist of 60 ~ 70% protein, 6 ~ 9% lipid and 15 ~ 20% carbohydrate.

The spirulina includes a variety of known spirulina species, including Spirulina platensis, Spirulina maxima, Spirulina subsalsa , Spirulina major, Spirulina geitleri, Spirulina siamese, Spirulina princeps , Spirulina laxissima , Spirulina curta , Spirulina spirulinoides , Spirulina platensis , Spirulina maxima, Spirulina geitleri and Spirulina siamese .

The Spirulina contains proteins, carbohydrates, water-soluble dietary fibers as well as antioxidant coloring matter components (chlorophyll, carotenoid, picocyanin) and antioxidant enzyme (SOD). Vitamins include beta carotene, vitamin B1, B2, B6, B12, vitamin E, inositol, and folic acid. Calcium, iron, potassium, magnesium, zinc, manganese, selenium and germanium. It contains all the essential amino acids, linolenic acid and gamma linolenic acid are essential fatty acids.

The term " fermentation " refers to a process of decomposing an organic substance using an enzyme possessed by the fermentation strain itself.

It is known that the fermented raw material has an effect of at least 2 times to several tens of times greater than before fermentation. Fermented metabolites contain various amino acids, organic acids, and antioxidants that are good for skin, promoting skin metabolism and smooth skin texture. In addition, as the fermentation process proceeds, the particles become smaller and the toxicity such as heavy metals is decomposed, so that the absorption rate is good and the skin troubles and allergy side effects are alleviated.

The Spirulina fermented extract may be obtained by successively sterilizing, fermenting, extracting, filtering, and concentrating.

Specifically, Spirulina is dried, pulverized, mixed with purified water, and sterilized by heating at 120 to 125 ° C for 10 to 20 minutes. By sterilizing the spirulina, it is possible to prevent intrusion of other contaminants other than the fermentation strain in the subsequent fermentation step. Because spirulina has a high concentration of various nutrients, it can be contaminated with microorganisms that produce toxic substances during fermentation.

Therefore, spores of most microorganisms can be destroyed and sterilized for 10 to 20 minutes at a temperature range where the useful components contained in Spirulina are not destroyed.

If the sterilization is done at a temperature higher than 125 ° C or if the sterilization period is longer than 20 minutes, the useful ingredient contained in Spirulina may be destroyed. Also, if the sterilization time is less than 120 ° C or the sterilization period is shorter than 10 minutes, the harmful microorganisms and spores are not destroyed and may be contaminated in the subsequent fermentation step.

The sterilized spirulina may be inoculated with a fermentation strain and fermented under predetermined conditions.

Concretely, the fermentation conditions of the fermentation extract are not particularly limited, but can be obtained by fermentation at 23 to 27 캜, and fermentation can be obtained at 96 to 144 hours.

The content of the active ingredient in the extract may vary according to the fermentation temperature and the fermentation time of the fermentation extract, and thus the skin improving activity may significantly differ, so that the fermentation conditions are suitably controlled.

The "extract" is a natural extract using various natural products as an extraction raw material. If the extract is a substance in which a solvent and an extraction raw material are brought into contact with each other under specific conditions to separate the components contained in the raw material from the natural product as an active ingredient contained in the extraction raw material, Regardless of the kind of the component or the component.

For example, it may include extracts of components dissolved in a solvent from natural products by using water or an organic solvent, and specific components of natural products, such as oils obtained by extracting only specific components such as oil. The extract may be dried and pulverized, or may be obtained by various extraction methods known in the art such as a hot water extraction method and an ethanol extraction method.

Wherein the extract is selected from the group consisting of water, an anhydrous or lower alcohol having 1 to 4 carbon atoms, acetone, petroleum ether, ethyl acetate, butyl acetate, trichloromethane, dichloromethane, chloroform, hexane and 1,3-butylene glycol , And can be extracted with ethanol in particular.

The extraction ratio of the active ingredient contained in the raw material may be different depending on the polarity of the solvent. Since the ethanol is excellent in selectivity for extracting a physiologically active substance from a natural raw material, the ethanol extract can realize an optimal skin improving effect .

Since the water and ethanol have different polarities and the effective components to be extracted depend on the polarity, the concentration of ethanol can be appropriately controlled so that an optimal skin improving effect can be realized.

The extract may also contain an extract which has been subjected to a conventional purification process. For example, the extract may be subjected to various purification methods such as separation using an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity) Lt; RTI ID = 0.0 > fractions. ≪ / RTI >

The cosmetic composition may be formulated into at least one member selected from the group consisting of a skin, a lotion, a cream, a serum, an emulsion, an essence, a powder, a foundation, a spray, a mask pack, a sheet pack, a sleep pack, have.

The cosmetic composition may be formulated by a conventional method. The contents disclosed in Remington ' s Pharmaceutical Sciences, Mack Publishing Company, Easton PA in the formulation of the external preparation for skin can be referred to, and in the formulation of the cosmetic composition, International cosmetic ingredient dictionary, 6th ed (The cosmetic, Toiletry and Fragrance Association , Inc., Washington, 1995).

Specifically, the cosmetic composition may be prepared into a general emulsified formulation and a solubilized formulation. For example, lotion such as soft lotion or nutrition lotion; Lotion such as facial lotion, body lotion; Nourishing cream, moisture cream, cream such as eye cream; essence; Makeup ointment; spray; Gel; pack; Sunscreen; Makeup base; A foundation such as a liquid type, a solid type or a spray type; powder; Make-up removers such as cleansing creams, cleansing lotions and cleansing oils; Or a cleansing agent such as a cleansing foam, a soap, a body wash, and the like, but is not limited thereto. In addition, the external preparation for skin may be formulated into ointments, patches, gels, creams or sprays, but is not limited thereto.

The cosmetic composition of the present invention may be appropriately blended with other ingredients within the range of not compromising the object of the present invention depending on the kind of the formulation or the purpose of use, in addition to the essential ingredients in each formulation.

The cosmetic composition may contain a conventional acceptable carrier and may appropriately contain, for example, oil, water, a surfactant, a moisturizer, a lower alcohol, a thickener, a chelating agent, a colorant, no.

The acceptable carrier may vary according to the formulation. For example, when formulated into ointments, pastes, creams or gels, there may be used an animal oil, a vegetable oil, a wax, a paraffin, a starch, a tracer, a cellulose derivative, polyethylene glycol, silicon, bentonite, silica, talc, zinc oxide, Extracts may be used.

When the cosmetic composition is formulated with a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder or an extract thereof may be used as a carrier component. In the case of a spray, Propellants such as carbon, propane, butane or dimethyl ether.

When the cosmetic composition is formulated into a solution or emulsion, a solvent, a solubilizing agent or an emulsifying agent may be used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl benzoate, 1,3-butyl glycol oil may be used, and in particular, fatty acid esters of cottonseed oil, peanut oil, corn oil, olive oil, castor oil and sesame oil, glycerol aliphatic ester, polyethylene glycol or sorbitan may be used.

When formulated into a suspension, the cosmetic composition may contain, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the cosmetic composition is formulated into soap, it is preferable to use, as a carrier component, an alkali metal salt of a fatty acid, a fatty acid hemiester salt, a fatty acid protein hydrolizate, isethionate, a lanolin derivative, an aliphatic alcohol, a vegetable oil, Can be used.

The cosmetic composition may be a cosmetic or dermatological composition which is generally used in the art depending on the quality or function of the final product, such as a lipid, an organic solvent, a solubilizing agent, a thickening agent, a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizer, Surfactants, water, ionic or nonionic emulsifiers, fillers, metal ion sequestrants, chelating agents, preservatives, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, And may further contain adjuvants conventionally used in the field of cosmetics or dermatology, such as any other ingredient.

However, the adjuvant and the mixing ratio thereof can be appropriately selected so as not to affect the preferable properties of the cosmetic composition according to the present invention.

The present invention will be further described with reference to the following examples, but it should be apparent that the present invention is not limited by the following examples.

Production Example: Spirulina Fermented Extract

Spirulina was completely dried at room temperature and then pulverized. The above Spirulina pulverized product and 20 times volume of purified water were mixed and sterilized and extracted at a temperature of 121 ° C for 15 minutes.

The sterilized Spirulina was inoculated with 1% (w / v) of Marinobacter shengliensis isolated from Jeju Lava Seawater.

Spirulina was inoculated into a liquid fermenter and fermented at different temperatures and times.

The fermented spirulina fermented material was filtered through a 250 mesh and 3 탆 filter, concentrated under reduced pressure at 60 캜 and lyophilized to obtain a solid content of the extract.

Comparative Preparation Example 1: Spirulina extract

Spirulina was completely dried at room temperature and pulverized to obtain a pulverized product.

Twenty times as much volume of purified water was mixed with the above Spirulina pulverized product and extracted at a temperature of 121 ° C for 15 minutes.

The extract was filtered through 250 mesh and 3 mu m filters, concentrated under reduced pressure at 60 DEG C, and lyophilized to obtain a solid content of the extract.

Comparative Preparation Example 2: Spirulina fermentation extract

The fermented extract was prepared in the same manner as in the preparation example except that only the fermentation strain was changed to Bacillus subtilis .

Comparative Preparation Example 3: Spirulina fermented extract

The fermentation extract was prepared in the same manner as in the production example except that only the fermentation strain was changed to Lactobacillus plantarum .

Manufacturing example setting

The fermentation conditions of the Spirulina fermented extract (1000 μg / mL) were evaluated with different fermentation conditions as described below.

division Temperature (℃) Fermentation time (h) Production Example 1 25 24 Production Example 2 25 48 Production Example 3 25 72 Production Example 4 25 96 Production Example 5 25 120 Production Example 6 25 144 Production Example 7 30 24 Production Example 8 30 48 Production Example 9 30 72 Production Example 10 30 96 Production Example 11 30 120 Production Example 12 30 144 Production Example 13 35 24 Production Example 14 35 48 Production Example 15 35 72 Production Example 16 35 96 Production Example 17 35 120 Production Example 18 35 144

Experimental Example  1: Functional evaluation according to fermentation condition

Experimental Example  1-1. ABTS  Evaluation of free radical scavenging activity by measuring method

Samples of the preparation examples were suspended in purified water to evaluate free radical scavenging activity.

The ABTS method measures the degree of conversion of the antioxidant to a colorless ABTS at 732 nm by dissolving the blue-green radical, ABTS (2,2'-azinodi [3-ethylbenzthiazolne-6-sulfonic acid]).

The radical ABTS scavenging activity of the diluted samples at the concentration of 1000 μg / mL was measured (Table 2).

division ABTS Scatters (1000 μg / mL) Production Example 1 83.66% Production Example 2 90.23% Production Example 3 96.45% Production Example 4 98.09% Production Example 5 98.47% Production Example 6 98.32% Production Example 7 85.99% Production Example 8 91.45% Production Example 9 95.70% Production Example 10 95.34% Production Example 11 97.39% Production Example 12 95.23% Production Example 13 89.30% Production Example 14 90.62% Production Example 15 93.48% Production Example 16 93.87% Production Example 17 95.58% Production Example 18 94.13%

As shown in Table 2, the antioxidative activity of the samples of the present invention was evaluated to be the most excellent when cultured at 25 ° C for 96 to 144 hours, and the antioxidative activity was partially reduced when the culture conditions were different.

Experimental Example  1-2. Collagenase  Evaluation of anti-aging activity through inhibitory activity

The sample of the preparation example was suspended in purified water by concentration to evaluate the collagenase inhibition activity.

Pro-Leu-Gly-Pro-D-Arg (Sigma, USA) dissolved in 0.3 mg / mL of 4-phenylazobenzyloxycarbonyl-Pro-Leu-Gly-Pro-D-Arg (Sigma, USA) was added to 4 mM CaCl ₂ in 0.1 M tris- μL was mixed with 50 μL of the sample.

After adding 75 μL of 0.2 mg / mL collagenase (Sigma, U.S.A) for 20 min at room temperature, 250 μL of 6% (w / w) citric acid was added to stop the reaction.

1.5 mL of ethyl acetate was added and absorbance was measured at 320 nm. The inhibitory activity of collagenase was expressed by the absorbance reduction rate of the sample solution added and the non-added sample (Table 3).

division Collagenase  Inhibitory activity (1000 μg / mL) Production Example 1 52.09% Production Example 2 53.23% Production Example 3 55.94% Production Example 4 59.54% Production Example 5 60.80% Production Example 6 59.29% Production Example 7 52.28% Production Example 8 52.32% Production Example 9 55.06% Production Example 10 53.52% Production Example 11 52.30% Production Example 12 50.21% Production Example 13 50.13% Production Example 14 51.64% Production Example 15 57.78% Production Example 16 54.21% Production Example 17 52.65% Production Example 18 51.95%

The results are shown in Table 3. As shown in Table 3, the collagenase inhibitory activity of the samples of the Examples was found to be the best when cultured at 25 ° C for 96 to 144 hours.

Experimental Example  2 : Comparative Example  Functional evaluation based on setting

Among the fermented extracts obtained by different fermentation conditions, the samples having the best skin - improving activity were selected.

Referring to Experimental Example 1, the samples of Preparation Examples 4 to 6 exhibited excellent skin improving activity, and the samples of Preparation Example 5 (fermentation at 25 ° C for 120 hours) increased the skin improving activity in proportion to the concentration (FIG. 1 And 2).

On the other hand, the samples of Comparative Production Examples 2 and 3 were selected in the same manner as in Experimental Example 1, and the samples having the highest activity were selected by varying the fermentation conditions, and the following examples and comparative examples were set as shown in Table 4.

division Contents Example 1 Production Example 4 Example 2 Production Example 5 Example 3 Production Example 6 Comparative Example 1 Comparative Preparation Example 1 Comparative Example 2 Comparative Production Example 2 Comparative Example 3 Comparative Production Example 3

Experimental Example  2-1. Wrinkle improvement activity evaluation

In order to evaluate the effect of improving the wrinkles of the skin, the following experiment was conducted.

Adult women over 30 years old were divided into 6 groups. The subjects were uniformly applied cosmetics formulated in cream form after morning and evening cleansing for 4 weeks.

ANTERA 3D (Miravex, Ireland) was applied for evaluation of wrinkle improvement. Instrumentation was performed before the test material was used, after 2 weeks, and after 4 weeks use.

The same test person measured the left part of the left eye of each subject and the same area was measured by overlapping with the image measured before the test material for the reproducibility of the measurement.

The captured images were matched using ANTERA pro software, which is dedicated to ANTERA 3D, and the matched measurement sites were used for analysis.

The measured values were analyzed using the Indentation index, which is a measurement variable.

Table 5 shows the variation of the Wrinkles small value of the testees after 4 weeks according to the use of the cosmetics formulated into the samples of the examples and the comparative examples. The better the wrinkle improvement effect is, the larger the variation of the wrinkles small value is.

division Wrinkles small average Example 1 1.56 Example 2 1.59 Example 3 1.57 Comparative Example 1 0.87 Comparative Example 2 1.02 Comparative Example 3 0.98

Referring to Table 5, the cosmetic composition in which the samples of the examples were formulated exhibited remarkably excellent skin wrinkle-improving effect as compared with the comparative example. The above results suggest that the spirulina extract can be remarkably improved in skin wrinkle improving activity by fermentation of Marinobacter aglynisis.

Experimental Example 2-2. Antioxidant activity evaluation

The extracts of Examples and Comparative Examples were suspended in purified water by concentration to evaluate free radical scavenging activity.

The DPPH assay measures absorbance at 540 nm to the extent that the inhibitor decolorizes the stable radical DPPH (2,2-Diphenyl-1-picrylhydrazyl radical).

Samples of Examples and Comparative Examples were diluted to a concentration of 1000 μg / mL. 100 μL of 5 × 10 ^-4 M DPPH (2,2-Diphenyl-1-picrylhydrazyl) was added to 100 μL of the diluted solution, stirred for 1 minute, reacted at 25 ° C. for 30 minutes, (Table 6).

division DPPH Scatters (1000 μg / mL) Example 1 78.9% Example 2 81.4% Example 3 79.5% Comparative Example 1 62.1% Comparative Example 2 64.6% Comparative Example 3 63.4%

Referring to Table 6, the samples of the examples showed remarkably excellent free radical scavenging ability as compared with the comparative examples. The above results indicate that the antioxidant activity of the fermented Spirulina extract was significantly changed due to the difference of fermentation strains and that the antioxidant activity could be remarkably improved by fermentation of Marinobacter aglynisis.

It will be understood by those skilled in the art that the foregoing description of the present invention is for illustrative purposes only and that those of ordinary skill in the art can readily understand that various changes and modifications may be made without departing from the spirit or essential characteristics of the present invention. will be. It is therefore to be understood that the above-described embodiments are illustrative in all aspects and not restrictive. For example, each component described as a single entity may be distributed and implemented, and components described as being distributed may also be implemented in a combined form.

The scope of the present invention is defined by the appended claims, and all changes or modifications derived from the meaning and scope of the claims and their equivalents should be construed as being included within the scope of the present invention.

Claims (5)

Marinobacter shengliensis ) as an active ingredient. The method according to claim 1,
The fermented extract is obtained by fermenting at 23 to 27 占 폚. 3. The method of claim 2,
Wherein the fermentation extract is fermented for 96 to 144 hours. The method of claim 3,
Cosmetic composition for antioxidant or wrinkle improvement. 5. The method according to any one of claims 1 to 4,
The cosmetic composition is formulated as at least one selected from the group consisting of skin, lotion, cream, serum, emulsion, essence, powder, foundation, spray, mask pack, sheet pack, sleep pack, wash off pack and peel off pack.

Images