KR20190045455A - Cosmetic composition comprising Phragmites Communis Ferment Extract - Google Patents

Abstract

The present invention relates to a cosmetic composition, a quasi-drug and functional food composition comprising a Galactomyces fermented product of reed extract as an active ingredient. By using the Galactomyces fermented product of the reed extract according to the present invention as a material for functional cosmetics, it is possible to satisfy consumers′ demand for products derived from natural substances which are eco-friendly and have few side effects. Also, by fermenting reeds with Galactomyces, it is possible to develop products with technological competitiveness due to a synergistic effect of the two advantages of reed and Galactomyces yeast.

Description

A cosmetic composition comprising a fermentation product of a reed extract (Phragmites Communis Ferment Extract)

The present invention relates to cosmetic compositions, quasi-drugs and functional food compositions containing galactomith fermented product of reed extract as an active ingredient.

In recent years, consumers are increasingly interested in functional cosmetics, which are nature-friendly and have few side effects. Therefore, Korean cosmetics companies as well as cosmetics companies in the world have been developing cosmetics using various functional natural materials derived from natural products.

As research and development such as whitening functional cosmetics derived from natural products and functional cosmetics improving wrinkles have progressed actively at home and abroad, domestic and foreign researches on most simple natural materials have reached saturation.

Fermentation, which is a traditional technology, is a technology to make useful materials for human body by increasing effective ingredients, absorption rate and safety of various natural materials by using microorganisms. In this process, increase of active ingredient, decrease of molecular structure, generation of new parasites, , It is a tendency that the research direction is being changed by research and development based on the synergy effect of lowering the toxicity of natural products by using such fermentation technology.

    As a specific example of fermentation, Galactomyces is one of the natural yeasts fermented by the yeast, and it is found that the skin color of the artisan leavening in the brewery is fine, clear and elastic, and Galactomyces, The interest in the effectiveness of the fermented product and various active ingredients contained therein has increased. SK-II has been getting attention in the product. It has been started to get attention in SK-II products. It has various vitamins such as vitamin B1, B2, pantothenic acid, B6 and folic acid which gives skin moisturizing, whitening, pore and soothing effects, carbohydrates, And various minerals are abundant. The anti-aging action of nucleic acids, which contain more than seven times the amount of sardines, is known, and kojic acid, also known as a representative whitening agent, was first known in the process of fermenting koji with koji in the early 1900s. On the other hand, various natural enzymes such as glycosylation enzymes and proteases produced during the fermentation process are known to have effects such as antioxidation, anti-aging, whitening, skin moisturizing and the like by enhancing the production and absorption of effective substances.

   Reed refers to species that make up the reed Phragmites of the genus Poaceae. The reed (Phragmites communis) grows from the Arctic to the tropics along lakes, wetlands and streams. The leaves are about 1.5 ~ 5m wide. Feathery flowers bloom and stems are straight and smooth. Other plants, such as Arundo donax, Ammophila arenaria and Palaris and Calamagrostis plants, are also called reed in English and are not paddy plants Sparganium and Typha plants are also called reeds. Dried reed stalks for centuries have been used as materials for roofing and building materials, as well as for basketry, arrows, pens, and musical instruments. It is also harvested in order to obtain cellulose which is rich in reeds. There are three kinds of reed plants that grow in Korea: reed, P. japonicus, and P. karka. It is a perennial herb that grows mainly in wetlands and streams, and there are many beard roots in the nodes of the rootstock. Flowers bloom in September with a lot of branches. The male flower has hairs and a long avalanche. The grasshopper is similar to a reed, but unlike a reed, there is a stem that hovers over the earth. The large grasshopper grass grows only on Ulleungdo Island.

    On the other hand, no method has been disclosed to produce the synergistic effect of fermentation of the above-mentioned reed extract by using galactomies.

Accordingly, the present inventors have made efforts to find a cosmetic composition that is natural-friendly and has less side effects derived from natural products, and as a result, there is a synergy effect of two advantages of reed by fermentation by fermentation of reed extract of Galactomyces And confirmed the invention.

It is an object of the present invention to provide a cosmetic composition comprising a galactomith fermented product of a reed extract as an active ingredient.

It is still another object of the present invention to provide a quasi-drug composition comprising a galactomith fermented product of a reed extract as an active ingredient.

It is still another object of the present invention to provide a functional food composition containing a galactomith fermented product of a reed extract as an active ingredient.

In one aspect of the present invention, the present invention provides a cosmetic composition comprising a galactomith fermented product of a reed extract as an active ingredient.

Specifically, the cosmetic composition of the present invention may contain a galactomith fermented product of a reeds extract.

In the present invention, " Phellinus linteus " includes roots and stem portions called roots, and the root roots used in the herb are dried root roots of reeds and used for gastric motility, diuretic, and hemostasis. Nowadays, reeds are planted where pollutants such as heavy metals are introduced to remove pollutants. Reed extract is known to have elasticity and sedative properties on the skin.

In the present invention, " reed extract " refers to a preparation which is obtained by squeezing reed with an appropriate leaching solution and concentrating by evaporating the leaching solution. The reed extract is obtained by subjecting the reed meal to extraction with water, an organic solvent or a mixed solvent thereof And may include an extract, a diluent or concentrate thereof, or a dry powder of the extract or all the forms formulated with it.

Further, in the extraction method, a method such as hot water extraction, hot water extraction, cold extraction, warm-up extraction, pressure extraction, reflux cooling extraction, or ultrasonic extraction may be used.

In order to obtain a reed extract, it is preferably extracted with water, an organic solvent or a mixed solvent thereof. In the case of extraction using an organic solvent, an organic solvent such as methanol, ethanol, isopropanol, butanol, ethylene, acetone, hexane, ether, chloroform, ethyl acetate, butyl acetate, dichloromethane, N, (DMSO), 1,3-butylene glycol, propylene glycol, or a mixed solvent thereof. The active ingredient of the herbal medicine may be extracted at room temperature or warmed under the condition that the active ingredient is not destroyed or minimized. But it is not limited thereto. The extraction efficiency and the degree of loss of the active ingredient of the medicament may differ depending on the organic solvent to be extracted. Therefore, An appropriate organic solvent can be selected and used.

The solvent extract may further comprise a step of filtering the extract to remove suspended solid particles. Nylon, etc., or ultrafiltration, freezing filtration, centrifugation, etc. may be used.

Concentration of the extract can be carried out by reduced-pressure concentration, reverse osmosis concentration, or the like.

The concentration and drying step includes freeze drying, vacuum drying, hot air drying, spray drying, vacuum drying, foam drying, high frequency drying, or infrared drying. In some cases, it may further comprise a step of pulverizing the finally dried extract.

In addition, the extract may be subjected to an additional fractionation process. For example, the extract is suspended in distilled water to obtain a nonpolar solvent soluble layer by extraction with a nonpolar organic solvent, for example, hexane, ether, dichloromethane, chloroform, ethyl acetate or a mixed solvent thereof, Concentrated and / or dried.

By fermenting the reed extract, a fermented product of the reed extract, which is an effective ingredient of the composition of the present invention, can be produced.

In the present invention, " fermentation " includes a process in which a microorganism or the like uses an enzyme contained therein to decompose an organic substance. Fermentation is one of the anaerobic respiration. Organisms that do anaerobic respiration do not completely decompose organic matter and produce other kinds of organic matter. By fermentation, the enzymes secreted by microorganisms and microorganisms spontaneously decompose organic substances, so that the size is reduced to 80 nm or less on average, thereby facilitating skin absorption. In addition to the strengthening of existing components due to fermentation, Can be increased. In addition, toxic substances such as heavy metals and pesticides which are harmful to human body by microorganisms are completely decomposed and harmless to the human body. The fermentation products are resistant to heat and carbon dioxide and do not change well, so that the preservability of active ingredients may be increased.

The microorganism used in the fermentation of the present invention is a yeast of the genus Galactomyces.

In addition, the fermentation method of the present invention can be carried out by a conventional fermentation method. Preferably, the microorganism used for the fermentation is inoculated with the organic material to be fermented and then cultured under a predetermined condition. The fermentation medium may be a conventional yeast fermentation medium, and may be supplied with an energy source of microorganisms by including yeast extract, glucose, sugar, syrup and the like.

The microorganism used in the fermentation of the present invention can be fermented by inoculating 0.1% (v / v) to 10% (v / v), preferably 1% (v / v) based on the total weight of the reed extract. The fermentation may be carried out at a temperature of 15 ° C to 45 ° C for 12 hours to 240 hours, preferably at a temperature of 21 ° C to 36.5 ° C for 20 hours to 120 hours, but is not limited thereto.

In addition, when the microorganism used for the fermentation is a yeast of the genus of Galactomyces, (i) 1 to 2% (v / v) glucose, 1 to 2% (v / v) 10 mg of the dried form of Galactomyces strain was added to 10 ml of fermentation medium containing 2% (v / v) of starch syrup and 0.1 to 0.5% (v / v) yeast extract, Pre-cultivation was performed; (Ii) adding 5 to 50% (v / v) of a reed extract to 250 ml of a medium having the same composition as the fermentation medium; (Iii) 2.5 ml of 1.0 ml of the culture medium is inoculated with the galactomycete precursor culture medium; (Iv) at a culture temperature of 19.5 to 25 占 폚; (V) culturing for 72 to 120 hours, a galactomithase fermentation product of the reed extract as an active ingredient of the present invention can be obtained.

The reed extract of the present invention can remove toxic components during fermentation by microorganisms, and can obtain a large amount of excellent active ingredients produced in yeast. In addition, the active ingredients are lowered in fermentation, Can be provided.

In addition, the galactomith fermented product of the reed extract of the present invention has a skin fibroblast proliferation effect for synthesizing collagen (Experimental Example 4). Finally, it was confirmed that the galactomith fermented product of the reed extract of the present invention had no irritation on the skin, and that the stability of the product after formulation was also excellent (Experimental Example 2 and Experimental Example 3).

In the present invention, " wrinkle improvement " means not only reducing the number of wrinkles of the skin or decreasing the depth, but also inhibiting skin aging through wrinkle improvement. To improve skin wrinkles, it can include materials that activate dermal fibroblasts and promote collagen production in the dermis. For the purpose of the present invention, the wrinkle improvement can be carried out through the production of collagen by galactomith fermentation of the reed extract, but the present invention is not particularly limited thereto, and the content of the galactomith fermented product of the reed extract, And is not particularly limited.

In the experimental example of the present invention, when the galactomized fermented product of the reed extract was added to the cultured human fibroblasts, the absorbance was higher than that of the ascorbic acid added to the human fibroblasts, And the galactomith fermented product of the reed extract showed excellent wrinkle-reducing effect (Experimental Example 4, FIG. 4).

The cosmetic composition of the present invention may comprise a galactomithase fermentation product of reed extract in an amount of 0.001 to 50% by weight, more preferably 0.2 to 20% by weight, most preferably 2 to 10% by weight, But is not limited to, galactomith fermentation in weight percent reed extract. When the content of the galactomith fermented product of the reed extract is less than 0.001% by weight, it is difficult to expect a significant effect of increasing the skin fibroblast proliferation. When the galactomized fermented product of the reed extract is more than 50.0% by weight, It is difficult to increase and the shape of the product and the stability of the product may cause some problems.

In addition, the cosmetic composition of the present invention may include, without limitation, commonly accepted ingredients other than the above-mentioned effective ingredients, and includes conventional auxiliary agents such as antioxidants, stabilizers, solubilizers, vitamins, pigments and fragrances, and carriers can do.

The cosmetic composition according to the present invention can be used as a cosmetic composition in the form of a solution, an ointment for external use, a cream, a foam, a nutritional lotion, a softening water, a pack, a soft water, an emulsion, a makeup base, But are not limited to, emulsions, pastes, gels, lotions, powders, soaps, surfactant-containing cleansers, oils, powder foundations, emulsion foundations, wax foundations, patches and sprays.

In addition, the cosmetic composition of the present invention may further comprise at least one cosmetically acceptable carrier to be incorporated in a general skin cosmetic composition, and examples thereof include oil, water, a surfactant, a moisturizer, A thickening agent, a chelating agent, a coloring matter, an antiseptic, a perfume, and the like may be appropriately compounded, but the present invention is not limited thereto. The cosmetically acceptable carrier to be contained in the cosmetic composition of the present invention varies depending on the formulations.

When the formulation of the present invention is an ointment, a paste, a cream or a gel, the carrier component may be an animal oil, a vegetable oil, a wax, a paraffin, a starch, a tracer, a cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide Mixtures of these may be used.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder or a mixture thereof may be used as the carrier component, Propellants such as fluorohydrocarbons, propane / butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, 1,3-butyl glycol oil may be used, in particular fatty acid esters of cottonseed oil, peanut oil, corn oil, olive oil, castor oil and sesame oil, glycerol aliphatic esters, polyethylene glycols or sorbitan may be used have.

When the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspension such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Crystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is a soap, an alkali metal salt of a fatty acid, a fatty acid hemiester salt, a fatty acid protein hydrolizate, isethionate, a lanolin derivative, an aliphatic alcohol, a vegetable oil, glycerol, .

When the formulation of the present invention is an interface-active agent-containing cleansing, the carrier component is selected from the group consisting of aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters.

In another aspect, the present invention provides a quasi-drug composition comprising a galactomith fermented product of a reed extract as an active ingredient.

Specifically, the galactomith fermented product of the reed extract of the present invention can be incorporated into the quasi-drug composition.

The reed extract and the fermented product thereof are as described above.

The term " quasi-drug " in the present invention means a fiber, a rubber product or the like used for the purpose of treating, alleviating, treating or preventing a disease of a human or an animal, a weak action on the human body, Or products similar to those which are not machinery, preparations used for sterilization, insecticides and similar uses for the prevention of infections, for the purpose of diagnosis, treatment, alleviation, treatment or prevention of diseases of human beings or animals Machinery, or apparatus, and that is not an apparatus, machine, or apparatus of an article used for the purpose of giving pharmacological effects to the structure or function of a person or animal, It also includes supplies.

When the composition of the present invention is contained in a quasi-drug for the purpose of skin improvement or the like, the composition may be used as it is or may be used together with other quasi-drugs, and may be suitably used according to a conventional method. The amount of the active ingredient to be mixed can be appropriately determined depending on the purpose of use.

The external preparation for skin may be prepared and used in the form of, for example, an ointment, a lotion, a spray, a patch, a cream, a powder, a suspension or an ointment.

In another aspect, the present invention provides a health supplement, a functional food, and a food additive composition containing a galactomith fermented product of a reed extract as an active ingredient.

Specifically, the galactomith fermented product of the reed extract of the present invention can be incorporated into the functional food composition.

The reed extract and the fermented product thereof are as described above.

In the present invention, the term " functional food " means a food group or a processed food which is imparted with added value to function or express the function of the food by using physical, biochemical, biotechnological techniques or the like. When the composition of the present invention is incorporated into a functional food for the purpose of skin improvement or the like, the composition may be used as it is, or may be used together with other functional food components, and may be appropriately used according to a conventional method. The amount of the active ingredient to be mixed can be appropriately determined depending on the purpose of use.

The formulation of the functional food is not particularly limited, but may be prepared and used in the form of, for example, a liquid, a granule, a powder, a capsule, a tablet, a pill, a suspension or an gel.

By using the galactomith fermented product of the reed extract according to the present invention as a material for functional cosmetics, it is possible to satisfy consumers' demand for a product derived from a natural product which is friendly to nature and less side effect, By fermenting, it is possible to develop products with technological competitiveness by synergy of two advantages of fermentation reed and galactomiss.

Fig. 1 is a spectrum showing the results of UV spectrum analysis of a fermentation medium containing a reed extract and a fermented product after fermentation with galactomith.
Fig. 2 is a photograph showing a result of performing QC / QA on the galactomith fermented product of the reed extract. Fig.
FIG. 3 is a graph showing the fibroblast cytotoxicity of the reed extract and the reed extract according to the treatment concentration of the galactomith fermented product. FIG.
Fig. 4 shows changes in absorbance (proliferative capacity) of fibroblasts treated with the fermentation extract of Galactomyces on ascorbic acid, reed extract and reed extract.
5 is a photograph showing the result of the microbial stability test of the formulation formulated as shown in Table 3. Fig.
FIG. 6 is a graph showing the stability of a formulation formulated as shown in Table 3 by a severe test, and shows a change in specific gravity of a preparation to which a galactomith fermented product of a reed extract was added.
FIG. 7 is a graph showing the stability of the formulation formulated as shown in Table 3 by the severe test, showing changes in the viscosity of a preparation to which a galactomith fermented product of a reed extract was added.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are for further illustrating the present invention, and the scope of the present invention is not limited by these examples.

Preparation of reed extract

0.1 kg of reed was washed thoroughly with distilled water, and about 10 times of distilled water was added thereto so that the total weight became 1 kg. Using the filter paper, only the extract was taken to produce the mushroom extract.

Fermentation of Galactomycetes by Reed Extract

A fermentation medium containing glucose, sugar, millet, and yeast extract was used to produce fermented products of the genus Galactomyces. The reed extract prepared in Example 1 was added to the medium and fermented by inoculating yeast. Fermentation conditions are shown in Table 1 below. Galactomyces cultures can increase glucose and sugar content to speed up proliferation.

Fermentation conditions of Galactomyces Fermentation condition Incubation temperature 21 ^o C Incubation time 72-120 hours Medium composition Glucose 1.5%, sugar 1.5%, starch syrup 1.5%, yeast extract 0.3% Reed extract 20% content

Experimental Example 1: Analysis of Galactomissae Fermentation

To analyze the compositional changes of galactomisses fermentation broth, the UV spectrometric method was used. The analysis conditions are shown in Table 2 below.

UV spectrum analysis conditions Analysis condition Detection Scanning from 200nm to 600nm

The results of the UV spectra of the fermented extract prepared after the fermentation with the galactomeys before and after the fermentation with the Galactomyces containing the reed extract of Table 1 were as shown in Fig.

According to the result of UV spectrum analysis of the culture medium of Galactomyces 3 days in a fermentation medium containing a reed extract, the center axis of the spectrum moved from 270 nm before fermentation to 290 nm before fermentation, Respectively.

Experimental Example 2: Confirmation of conformity to QC / QA protocol for commercialization

 The fermentation material was immersed in water at room temperature for 24 hours using 1,3-butylene glycol (1,3-Butylene Glycol), and the fermented material was filtered to recover the filtrate. As a result of confirming the suitability as the raw material of cosmetic materials according to the KFDA by conducting the QC / QA on the standards and specifications of the KFDA standards, characteristics, pH, specific gravity, microbial stability, lead / arsenic content, (Fig. 2).

Experimental Example 3: Cytotoxicity of Galactomith fermented by Reed extract

 The cytotoxic effect of the reed extract on the collagen-forming human fibroblast of the skin dermal layer of the galactomith fermented product was examined. Specifically, human fibroblasts cultured in DMEM medium were seeded into 96-well plates at 10,000 cells per well. The reed extract of Experimental Example 1 and the galactomithase fermentation product of the reed extract were respectively added to 0.125% to 8% and cultured for 72 hours. After incubation, Easytox kit reagent was used to stain living cells. One hour later, absorbance was measured at 450 nm using an ELISA reader (FIG. 3).

As a result, in case of Galactomith fermented with reed extract, no toxicity was observed even at the highest concentration of 8%, whereas in case of reed extract, 75% of fibroblasts were killed by 8% Suggesting that cellulosic toxicity of galactomith extract is reduced by decomposition or transformation of galactomithase fermented materials. In addition, it suggests that the wrinkle improving effect is enhanced by increasing the fibroblast growth rate from 100% to 150% from 0.125% to 8% addition of the galactomized fermented product of the reed extract.

Experimental Example 4: Improvement of wrinkles of Galactomith fermented by Reed extract

 The effect of improving the wrinkles was examined by measuring the proliferative capacity of human fibroblasts, which are collagen-forming cells of the dermal layer of the skin by the fermentation of galactomith of reed extract. Specifically, human fibroblasts cultured in DMEM medium were seeded into 96-well plates at 10,000 cells per well. As a positive control, ascorbic acid, which is known to have the effect of improving skin wrinkles, was diluted to an optimal concentration of 0.005%, and the Galactomithase fermented product of Reed Extract and Reed Extract of Experimental Example 1 3%, respectively, and the proliferative activity of fibroblasts was examined by the method of Example 3 (Fig. 4).

As a result, the reed extract obtained in Example 2 exhibited the highest absorbance at the time of addition of the galactomith fermented product, showing excellent skin cell proliferation efficiency, which was higher than that of the ascorbic acid added as the positive control. This suggests that the galactomith fermented product of the reed extract proliferates the skin cells and has an excellent wrinkle-reducing effect.

Experimental Example 5: Formulation and stability evaluation of Galactomith fermented product of Reed extract

Various emulsifiers, thickeners, surfactants, moisturizers and preservatives can be formulated into compositions having the compositions shown in Table 3 below.

Furtherance weight % Purified water Balance glycerin 7 Triethanolamine 5 Butylene glycol 4 Sheer butter 4 Cyclopentasiloxane 4 PEG-100 stearate 4 Cetearyl alcohol 3 Petrolatum 3 Polysolphate-16 3 Disodium iodide 3 Dimethicone 3 Phenylalanine 3 Hydroxycellulose 2 Carbomer 2 Galactomith fermentation of reed extract 2

As a result of examining the microbial stability of the formulation, it was found that the microorganisms were not found in all of the conditions 1, 2, and 3 in which the formulation was treated (FIG. 5).

On the other hand, in order to investigate the stability by the harshness test, the formulations were stored at 50 ° C, room temperature, and -20 ° C for 12 days repeatedly, and changes in viscosity and specific gravity were examined (FIGS. 6 and 7).

As a result of the investigation on the stability according to the severe condition, it was found that (i) the variation of the specific gravity of the formulation was stable with almost no change within 0.05% (FIG. 6), (ii) The viscosity of the formulation was stable by having a variation width of 10 or less as compared with the basic formulation (Fig. 7). This suggests that the galactomires fermented product of the reed extract has excellent formulation stability.

From the above description, it will be understood by those skilled in the art that the present invention may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. In this regard, it should be understood that the above-described embodiments are to be considered in all respects as illustrative and not restrictive. The scope of the present invention should be construed as being included in the scope of the present invention without departing from the scope of the present invention as defined by the appended claims.

Claims (6)

A cosmetic composition for wrinkle improvement, comprising a fermented yeast extract of Reed as an active ingredient, wherein the yeast is a yeast of Galactomyces genus. The composition according to claim 1, wherein the reed extract is extracted with water, an alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof. The composition of claim 1, wherein the yeast fermented product of the reed extract is 0.5 to 10% by weight based on the weight of the total composition. The composition according to claim 1, wherein the fermentation product is fermented by a yeast of the genus Galactomyces under the following conditions.
(a) a culture temperature of 19.5 DEG C to 25 DEG C;
(b) a culturing time of 72 to 120 hours;
(c) from 1 to 2% (v / v) glucose, from 1 to 2% (v / v) sugar, from 1 to 2% (v / v) Yeast extract; And
(d) 5 to 50% (v / v) reed extract. A quasi-drug composition for improving wrinkles, comprising a galactomith fermented product of a reed extract as an active ingredient, wherein the yeast is a yeast of Galactomyces genus. A functional food composition for improving wrinkles, comprising a galactomith fermented product of a reed extract as an active ingredient, wherein the yeast is a yeast of the genus Galactomyces.

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