KR20130023534A - Cosmetic composition for the skin desquamation comprising the citrus junos extracts - Google Patents

Abstract

PURPOSE: A cosmetic composition containing a Citrus junos extract is provided to improve a skin peeling effect. CONSTITUTION: A cosmetic composition for skin peeling contains a Citrus junos extract. The Citrus junos extract is processed by fermentation using microorganisms. The microorganism is Saccharomyces cerevisiae as yeast. The microorganism is selected from a group consisting of Lactobacillus, Bifidobacterium, Streptococcus, Leuconostoc, Pediococcus, Lactococcus, and a mixture thereof.

Description

Cosmetic composition for exfoliating the skin containing citron extracts {Cosmetic Composition for the Skin Desquamation Comprising the Citrus junos Extracts}

 The present invention relates to a cosmetic composition comprising a citron extract, and more specifically, the citron extract obtained by treating the citron processed by citron and fermentation method or foaming method by various methods such as solvent extraction method, ultrasonic extraction method and supercritical extraction method. It relates to a skin exfoliation cosmetic composition comprising and a cosmetic method.

The skin is divided into three parts: the upper layer of skin, the epidermis, the dermis, and the subcutaneous fat layer. The double epidermis is further subdivided into stratum corneum, granule layer, pole layer, and basal layer. The epidermal cells of the basal layer are differentiated as they rise to the upper part of the skin and finally reach the stratum corneum. Epidermal cells that reach the stratum corneum lose their nuclei and are converted to dead cells as they are filled with an insoluble protein called keratin. The stratum corneum consists of differentiated epidermal cells (skin cells) and skin lipids that fill between them, which protects the body from outwards and protects the body from external physical, chemical and biological stimuli. . The keratinocytes are connected by a protein called desmosome, and as they rise up the stratum corneum, the desmosome is broken down to weaken the cohesion between keratinocytes and eventually the keratinocytes are separated from the skin. The stratum corneum consists of 15 to 20 layers for normal skin and the turn-over period when they are completely separated takes about 4 weeks for normal skin. However, aging skin, dry skin, acne skin, etc., the separation of the stratum corneum is slower than normal to produce abnormal keratinocytes, the stratum corneum is thickened to the surface of the skin, rough and elasticity is reduced. In addition, the melanin over-produced by the external environment is not properly discharged, the skin becomes dull.

The stratum corneum, roughened by internal and external factors, promotes exfoliation and increases the activity and regeneration of cells under the stratum corneum, resulting in a transparent and smooth skin. Retinoic acid and alpha hydroxy acids (AHA) are known to be effective in promoting exfoliation. They are also effective in removing wrinkles, synthesizing collagen, and skin regenerating ability, but in order to obtain the above cosmetic effect, alpha hydroxy acid must be contained in high concentration (1.0 ~ 10%) or strong acidity (below pH 3.5) do. However, when using cosmetics containing high concentrations or strongly acidic alpha hydroxy acid may cause skin irritation such as erythema, itching, itching, skin abnormalities or burns. In addition, the use of alpha hydroxy acid increases the sensitivity to sunlight, and eventually skin irritation such as phototoxicity and photosensitivity may occur. Therefore, there are many researches on raw materials and compositions that can remove dead skin cells and improve skin dullness without irritation with regard to exfoliation and skin cell regeneration. However, there are no raw materials with excellent exfoliation and cell regeneration as these raw materials. to be.

The present inventors searched for various skin physiological activities of natural plant components in order to prevent skin aging and promote skin exfoliation, the result of the process of extracting citron from natural plant extracts, such as citron and fermentation method or foaming method, solvent extraction, ultrasonic extraction, supercritical The present invention was completed by confirming that the extract obtained by extraction by various methods such as extraction method promotes exfoliation of the skin to make the skin clean and shiny.

That is, an object of the present invention is to provide a cosmetic composition for exfoliation of the skin containing citron extract.

In the present invention, to provide a cosmetic composition for exfoliation using citron, citron extract was prepared using various extraction methods and applied to the product. To this end, the present inventors extracted the citrons processed by citron and fermentation method or foaming method using a general solvent, or prepared the citron extract obtained by using ultrasonic extraction method, supercritical extraction method, etc. as an active ingredient. The object is achieved by a cosmetic composition for exfoliation of the skin containing citron extract as an active ingredient.

Preferably, the citron extract is characterized in that it contains 0.00001 to 25.0% (v / v) based on the total weight of the cosmetic composition. That is, the content of citron extract is less than 0.00001%, the effect is insignificant, and the skin irritation may be induced at 25.0% or more to limit the content.

In addition, the citron extract is preferably citron and citron processed by fermentation or foaming method such as water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, propylene glycol, butylene glycol, glycerin, acetone, ethyl acetate, butyl Solvent extraction using an extraction solvent selected from the group consisting of acetate, chloroform, diethyl ether, dichloromethane, hexane and mixtures thereof; Ultrasonic extraction method; It is characterized by extracting using a supercritical extraction method.

In addition, preferably, the fermentation method is characterized in that the citron is cultured using the yeast Saccharomyces cerevisiae (Saccharomyces cerevisiae).

Also preferably, the cosmetic composition consists of a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray It is characterized by having a formulation selected from the group.

Another object of the present invention is achieved by a cosmetic method characterized by promoting the exfoliation of the skin by applying the above cosmetic composition to the skin.

The cosmetic composition according to the present invention promotes the exfoliation of keratin generated in the skin by adding the citron extract to the cosmetic, thereby obtaining a very excellent exfoliation effect of the skin.

In order to achieve the above object, the present invention provides a skin exfoliation cosmetic composition comprising a citron extract.

Citron (柚子) used as an active ingredient in the composition of the present invention is a fruit of citrus junos ( Ctrus junos ) is a ball-shaped one side and has a diameter of 4 ~ 7cm. The color is light yellow and the shell is bumpy. The fragrance is good and the pulp is soft but the acidity is strong. It is native to the Yangtze River in China. There are kinds of oil cleaners, yellow oil and oil. Produced in Korea, China, and Japan, Korea has the strongest fragrance and thickest skin. The major mountainous regions in Korea are Goheung, Wando, Jangheung, Jindo and Geoje, Namhae, and Tongyeong, Jeollanam-do.

On the other hand, the term "yuzu" in the present specification means a variety of parts "branch, roots, leaves, flowers" of the citron, especially the fruit portion of the present invention was completed.

     Citron extract of the present invention can be obtained according to a conventional method known in the art, that is, using a conventional solvent, under the conditions of conventional temperature and pressure, preferably water, anhydrous having 1 to 4 carbon atoms or One or more extraction solvents selected from the group consisting of hydrous lower alcohols (e.g. methanol, ethanol, propanol and butanol), acetone, ethyl acetate, chloroform, butyl acetate, 1,3-butylene glycol and mixtures thereof It can be obtained using, more preferably using ethanol, 70% (v / v) aqueous solution of ethanol or water, and most preferably can be obtained using 70% ethanol aqueous solution.

On the other hand, it is apparent to those skilled in the art that the citron extract of the present invention can obtain an extract having substantially the same effect using not only the aforementioned extraction solvent but also other extraction solvents.

According to a preferred embodiment, the method for preparing citron extract of the present invention is as follows. First, the citron is washed with purified water, dried and crushed into small pieces so that the particle size is 300 mesh or less, and then water of 1 to 10 times the dry weight, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, acetone , Ethyl acetate, butyl acetate or 1,3 butylene glycol are added. After cooling condenser is installed to prevent evaporation of the active ingredient in the state to extract for 3 to 20 hours at 40 ~ 100 ℃, or extract for 1 to 15 days at 4 ~ 40 ℃ and completely dried extract by rotary evaporation Manufacture. In this case, 1,3 butylene glycol is difficult to dry using a rotary vacuum evaporator, so that the water content is adjusted by drying in a hot air dryer at 30 ° C. to 60 ° C. such that the drying loss is 1% (w / v) after extraction under the above conditions. It is used for the cosmetic of this invention.

On the other hand, the citron extract of the present invention includes not only the extract by the extraction method described above, but also an extract by supercritical extraction or ultrasonic extraction by reduced pressure and high temperature with carbon dioxide. The decompression by carbon dioxide and the supercritical extraction by high temperature mean supercritical fluid extraction. Generally, the supercritical fluid is a liquid having a gas when the gas reaches a critical point under high temperature and high pressure. It is gaseous, chemically similar in polarity to nonpolar solvents, and supercritical fluids are used for the extraction of fat-soluble substances ( J. Chromatogr . A. 1998; 479: 200-205).

Carbon dioxide becomes a supercritical fluid with both liquid and gaseous properties as the pressure and temperature reach a critical point through the operation of a supercritical fluid device, resulting in increased solubility in fat-soluble solutes. When supercritical carbon dioxide passes through an extraction vessel containing a certain amount of sample, the fat-soluble substance contained in the sample is extracted from the supercritical carbon dioxide.

When the supernatant carbon dioxide containing a small amount of cosolvent is passed through the sample remaining in the extraction vessel after extracting the lipid-soluble substance, components that were not extracted by pure supercritical carbon dioxide can be extracted.

The supercritical fluid used in the supercritical extraction method of the present invention can effectively extract the active ingredient by using a supercritical carbon dioxide or a mixed fluid in which a co-solvent is added to carbon dioxide. Such cosolvents may be selected from the group consisting of chloroform, ethanol, methanol, water, ethyl acetate, hexane diethyl ether and mixtures thereof.

Most of the extracted samples contain carbon dioxide. Since carbon dioxide is volatilized into air at room temperature, the extract obtained by the above method may be used as a cosmetic composition, and the cosolvent may be removed by a reduced pressure evaporator.

As an example of the method for preparing citron extract by supercritical extraction method, the citron is washed with purified water, dried and crushed into smaller pieces of 300 mesh or less, and then the sample is placed in a supercritical extraction vessel and used with a liquid chromatography pump. In this way, pure carbon dioxide can be flowed in a supercritical state at a suitable temperature and pressure to obtain a citron extract in a test tube.

In supercritical extraction, carbon dioxide is at a temperature of 35 to 100 ° C., preferably 50 to 70 ° C., more preferably about 60 ° C., 100 to 500 atmospheres, preferably 200 to 400 atmospheres, more preferably about 300 Supercritical is recommended under pressure. In other words, when the pressure is less than 100 and more than 500 atm, the content of the agonist in the extract is very low, making it difficult to show the effect, and the extract between the two atm shows an excellent effect.

As an embodiment of the method for preparing citron extract by ultrasonic extraction, the citron or crushed citron is put in an extraction solvent selected from chloroform, ethanol, methanol, water, ethyl acetate, hexane, diethyl ether and mixtures thereof, and then ultrasonically Extractor (Supernonic, United States of America) can be extracted for about 1 to 6 hours at 30 ℃ with an intensity of 25KHz, The ultrasonic extraction time may vary depending on the amount of sample to be extracted.

The extraction solvent that can be used in the ultrasonic extraction method may be selected from the group consisting of chloroform, ethanol, methanol, water, ethyl acetate, hexane, diethyl ether and mixtures thereof.

The extracted sample is vacuum filtered to recover the filtrate, and then removed by a vacuum evaporator, the extract can be obtained through a conventional extract preparation method of freeze drying.

In addition, the citron extract of the present invention also includes an extract that has undergone a conventional purification process. Obtained by various additional purification methods, such as, for example, separation using an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity). The fraction is also construed as being included in the citron extract of the present invention.

In addition, the citron extract of the present invention also includes a citron extract undergoing a fermentation process. It is interpreted that fermented products obtained by fermentation with a bacterium selected from the group consisting of yeast, lactic acid bacteria, Bacillus bacteria and mixtures thereof as a fermentation process are included in the citron extract of the present invention.

Yeast used to ferment the citron of the present invention belongs to eukaryotic microorganisms (organisms in which the nucleus and the cytoplasm are divided by the nuclear membrane) and have cell organelles such as cell walls, cell membranes, nuclei, private bodies, and granules. Those without sex distinction breed primarily by budding, among which many of the isolated yeasts have no fermentation capacity, multiply by fission or bud-fission instead of budding, and ascospore. There are also various groups of yeast, such as the formation of spores (ballistospore) or no spore formation is observed. The size is usually 5 × 5 ^-12 ~ 10 × 5 ^-12 ㎛, 5-10 times the size of bacteria, and the optimal pH survives at 4.5-5.5 but 1-10. Yeasts that survive 12 hours at pH 1 are more acid resistant than bacteria, so adjusting the pH of the medium to about 3.5 to 3.8 can reduce bacterial contamination.

Yeast is slightly different depending on the species, but most grow well at 20 ~ 25 ℃. Usually, it is killed above 50 ℃, but it can be overcome to some extent by adding the growth factor which is not synthesized above the temperature directly to the medium. Saccharomyces cerevisiae dies at over 40 ° C, but this can be prevented by adding oleic acid and ergosterol to the medium. Yeast cells themselves are single-celled plants that contain large amounts of protein and trace minerals. A preferred yeast for use in fermenting the citron of the present invention is Saccharomyces cerevisiae .

In addition, lactic acid bacteria that can be used to ferment citron in the present invention is a kind of microorganisms very beneficial to the human body to break down carbohydrates such as glucose or lactose to produce organic acids such as lactic acid (lactic acid) or acetic acid. Generally, lactic acid bacteria make lactic acid from sugar is called fermentation, and fermented food is made through this fermentation process. Is the lactic acid bacteria that can be used in the present invention is preferably Lactobacillus bacteria (Lactobacillus), Streptococcus (Streptococcus), bifidobacteria tumefaciens (Bifidobacterium), flow Pocono stock (Leuconostoc), Phedi O Rhodococcus (Pediococcus), Lactococcus (Lactococcus) And mixtures thereof, most preferably Lactobacillus . Among the Lactobacillus ( Lactobacillus ) is the most preferred lactic acid bacteria to be used to ferment the citron of the present invention is Lactobacillus plantarum ( Lactobacillus plantarum ).

In addition, Bacillus (Bacillus) bacteria can be used as a fermentation bacterium used to ferment citron in the present invention, Bacillus is a spore-forming bacteria, inhabit in soil or water, and most of them are related to decomposition of organic matter. Bacillus bacteria that can be used in the present invention is preferably Bacillus subtilis ( Bacillus subtilis ), Bacillus pumilis ), Bacillus licheniformis ), Bacillus megaterium megaterium), Bacillus poly flops mentee carcass (Bacillus polyfermenticus), Bacillus mesen Terry carcass (Bacillus mesentericus ) and mixtures thereof, and most preferably Bacillus subtilis , which is also known as Bacillus subtilis , which anaerobicly metabolizes various sugars such as glucose, starch, etc. It is an organic substance degrading microorganism that is also used in the manufacture of fermented foods such as.

Moreover, the citron extract of this invention also contains the extract obtained using the preparation method. Foaming agent is a pharmaceutical technology that changes the original properties of the medicine by processing the medicine based on the herbal theory. Mainly refers to the process of steaming, boiling, roasting, roasting or roasting or a process in which these are mixed.

In the present invention was used after steamed and dried citron and to prepare an extract using this. In addition to the solvent extraction method, the processed citron using the foaming method to steam and dry the citron can be extracted not only by the extraction method by the supercritical extraction method or the ultrasonic extraction method by reduced pressure with carbon dioxide and the high temperature, and according to the embodiment of the present invention. Extract with% ethanol.

Citron extract of the present invention may be prepared in a powder state by an additional process such as vacuum distillation and freeze drying or spray drying.

     In addition, according to a preferred embodiment of the present invention, the content of the citron extract of the present invention is 0.00001 to 25.0% by weight, preferably 0.0001 to 20.0% by weight relative to the total weight of the cosmetic composition. At this time, when the total weight of the citron extract is less than 0.00001% by weight, the effect is less likely to appear, when it exceeds 25.0% by weight is likely to cause irritation to the skin and may have a significant effect on the stabilization of the formulation.

The components included in the cosmetic composition of the present invention may include components conventionally used in cosmetic compositions in addition to the citron extract as an active ingredient, for example, conventional adjuvants such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavorings. And a carrier.

Cosmetic compositions of the present invention may be prepared in any formulation conventionally prepared in the art, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing , Oils, powder foundations, emulsion foundations, wax foundations and sprays, and the like, but are not limited thereto. More specifically, it may be prepared in the form of a flexible lotion (skin), nutrition lotion (milk lotion), nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder. .

When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

In the case where the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. Especially, in the case of a spray, a mixture of chlorofluorohydrocarbons, propane / Propane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 , 3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan and the like can be used.

In the case where the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives, or ethoxylated glycerol fatty acid esters.

In addition, the present invention provides a makeup method characterized in that to clean and smooth the skin using a skin exfoliation cosmetic composition comprising a citron extract.

The cosmetic process of the present invention refers to all the cosmetic processes for applying the cosmetic composition of the present invention to human skin. That is, all methods known in the art for applying the cosmetic composition to the skin belong to the cosmetic method of the present invention.

The cosmetic composition of the present invention may be used alone or in combination, or may be used by overlapping with other cosmetic compositions other than the present invention. In addition, the cosmetic composition excellent in the exfoliation effect of the skin according to the present invention can be used according to a conventional method of use, the number of times of use can be varied according to the user's skin condition or taste.

When the cosmetic composition of the present invention is a soap, a surfactant-containing cleansing or a surfactant-free cleansing formulation, it may be wiped off after application to the skin, or may be washed off with water. As a specific example, the soap is liquid soap, powdered soap, solid soap and oil soap, the surfactant-containing cleansing formulation is a cleansing foam, cleansing water, cleansing towel and cleansing pack, the surfactant-free cleansing formulation is a cleansing cream , Cleansing lotion, cleansing water and cleansing gel, but are not limited thereto.

When the cosmetic method including the citron extract of the present invention is applied to a human skin, a cosmetic method may be performed, thereby removing the dead skin cells and exfoliating the skin.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are only for describing the present invention in more detail and that the scope of the present invention is not limited by these embodiments in accordance with the gist of the present invention .

Example 1: Citron Extract Preparation

To 200 g of the dried citron fruit, 1,000 ml of an aqueous 70% ethanol solution in which the ratio of water and ethanol was adjusted to 7: 3 was added and leached at room temperature for 4 days. It was filtered and the filtrate was concentrated in vacuo to give a dry extract, the extraction yield was 19.8g / 200g. The dried extract was dissolved in 50% 1,3-butylene glycol solution to a concentration of 1% (w / v), filtered and applied to the experiment of the present invention.

Example 2-Example 14 Preparation of Citron Extract

Examples 2 to 14 were extracted in the same manner as in Example 1 except that the extraction solvent of Table 1 was used to obtain a citron extract.

Extraction yield of citron extract division Extraction solvent From 1 kg of citron
Weight of extract obtained in g Example 2 Purified water 18.4 Example 3 25% ethanol aqueous solution 18.8 Example 4 50% aqueous ethanol solution 18.4 Example 5 100% ethanol 17.7 Example 6 80% methanol aqueous solution 16.5 Example 7 100% methanol 15.8 Example 8 100% acetone 16.7 Example 9 100% diethyl ether 17.4 Example 10 100% Hexane 15.5 Example 11 100% normal-propanol 19.3 Example 12 100% isopropanol 18.6 Example 13 100% normal-butanol 17.5 Example 14 100% ethyl acetate 18.7

Example  15: Preparation of Citron Extract

The dried citron fruit was washed with purified water, dried and crushed into small pieces, and then 1 g of the sample was placed in an extraction container (Supercritical Fluid extractor, Jasco, Japan), and pure carbon dioxide was added at a temperature of 60 ° C. using a liquid chromatography pump. At 300 atm under pressure, the supernatant was put in a 10 mL glass test tube for up to 50 minutes every 5 minutes while flowing with 10% by volume of 95% alcohol. In the preliminary experiment, 60 ° C. and 300 atm showed the best results for the supercritical extraction. In the present invention, this condition was used for the experiment. The extract was dried with a rotary evaporator to obtain a dry weight of 5.2 g / 100 mL. The dried extract was dissolved in 50% 1,3-butylene glycol solution and used again.

Example  16: Preparation of Citron Extract

200 g of the dried citron fruit was crushed into small pieces and placed in purified water, and then extracted at 30 ° C. for about 2 hours at an intensity of 25 KHz using an ultrasonic extractor (Supernonic, United States). It was filtered and the filtrate was concentrated in vacuo to give a dry extract, the extraction yield was 10.2g / 200g. The dried extract was dissolved in 50% 1,3-butylene glycol solution to a concentration of 1% (w / v), filtered and applied to the experiment of the present invention.

Example  17: Preparation of Citron Yeast Fermented Extract

The citron fruit was washed with purified water and dried to make fine powder using 300 mesh. This was added to yeast culture medium (Potato Dextrose Broth, Accumedia, United States) to 10 g / L.

Saccharomyces cerevisiae was used as a yeast strain for fermentation, and was added at 50,000 cfu / L per culture. The culture was carried out using a 5L fermenter for 7 days, maintained at 30 ℃, pH 5-7. After culturing, the fermented yeast fermentation broth was centrifuged to remove primary cultures, followed by final filtration using 0.25 μM filter paper. The filtrate thus obtained was aged again in a cold warehouse at 4 ° C. for about 10 days, and the filtrate was filtered again using a filter of 0.25 μM, and used for the present invention. Called. Citron yeast fermentation extract used in the following experiment was used to maintain a final concentration of 1g / 100mL.

Example  18: Preparation of Citron Lactic Acid Bacteria Fermented Extract

The citron fruit was washed with purified water and dried to make fine powder using 300 mesh. Add 10 g / L of lactic acid bacteria culture medium (Lactobacillus MRS Broth, Difco, USA) and mix After sterilization at 121 ℃ was inoculated with lactic acid bacteria.

Lactobacillus used for fermentation was used Lactobacillus plantarum ( Lactobacillus plantarum ), was added at 50,000 cfu / L per culture. Cultivation was carried out using a 5L fermenter for 3 days, maintained at 30 ℃, pH 5-7. The cultured citron fermentation broth was centrifuged to remove cultures first, and then filtered through 0.25 μM filter paper. The filtrate thus obtained was aged for about 10 days in a low temperature warehouse at 4 ° C., and the filtrate was filtered again using a filter of 0.25 μM and used for the present invention. Called. Citron lactobacillus fermented extract used in the following experiment was used to maintain a final concentration of 1g / 100mL.

Example  19: Citron Bacillus  Preparation of Fermented Extract

The citron fruit was washed with purified water and dried to make fine powder using 300 mesh. This was added to the Bacillus culture medium at 10 g / L.

Bacillus strains used for fermentation were used Bacillus subtilis ( Bacillus subtilis ), was added at 50,000 cfu / L per culture. Cultivation was carried out using a 5L fermenter for 3 days, maintained at 30 ℃, pH 5-7. The cultured citron fermentation broth was centrifuged to remove cultures first, and then filtered through 0.25 μM filter paper. The filtrate thus obtained was aged for about 10 days in a low temperature warehouse at 4 ° C., and the filtrate was filtered again using a filter of 0.25 μM and used in the present invention. Called. Citron Bacillus fermented extract used in the following experiment was used to maintain a final concentration of 1g / 100mL.

Example  20: Forbidden  Preparation of Citron Extract

Boiled, steamed, roasted, roasted or baked or a mixture thereof to obtain the extract of citron of the present invention was carried out as follows. That is, after the powdered citron fruit washed and dried with purified water and finely made using 300 mesh, steaming was carried out under a condition of 60 ℃ ~ 150 ℃, 10 minutes ~ 2 hours and then dried. Citron extract thus obtained was extracted using a 70% ethanol solution.

The following prescription examples are provided, but the prescription of the present invention is not limited thereto.

Citron extract of the following formulation example can be prepared by applying all the citron extract prepared in Examples 1 to 20.

Prescription 1: Softener

Prescription examples of softening water in cosmetics containing citron extract are as follows.

ingredient content(%) Citron Extract 0.5 glycerin 5.0 1.3-butylene glycol 3.0 Allantoin 0.1 DL-Panthenol 0.3 Lee D. TEE-2NA 0.02 Benzophenone-9 0.04 Sodium hyaruronate 5.0 Nicole HCO 60 0.4 Methyl paraben 0.2 incense, 0.01 Distilled water Balance Sum 100

Prescription Example 2: Nutritional Cosmetics

 Prescription examples of nutrient cosmetics in cosmetics containing citron extract are as follows.

ingredient Content (unit: weight%) Citron Extract 2.0 Glyceryl stearate SE 1.5 Stearyl alcohol 1.5 lanolin 1.5 Polysorbate 60 1.3 Sorbitan stearate 0.5 Hardened vegetable oil 1.0 Mineral oil 5.0 Squalane 3.0 Trioctanoine 2.0 Dimethicone 0.8 Tocopherol Acetate 0.5 Carboxyvinyl polymer 0.12 glycerin 5.0 1.3-butylene glycol 3.0 Sodium hyaluronate 5.0 Triethanolamine 0.12 Methyl paraben 0.2 incense 0.02 Distilled water Balance Sum 100

Prescription example  3: Nourishing cream

 Prescription example of nutrition cream among cosmetics containing citron extract is as follows.

ingredient Content (unit: weight%) Citron Extract 3.0 Lipophilic monostearate 2.0 Cetearyl alcohol 2.2 Stearic acid 1.5 Wax 1.0 Polysorbate 60 1.5 Sorbitan stearate 0.6 Hardened vegetable oil 1.0 Squalane 3.0 Mineral oil 5.0 Trioctanoine 5.0 Dimethicone 1.0 Sodium magnesium silicate 0.1 glycerin 5.0 Betaine 3.0 Triethanolamine 1.0 Sodium hyaluronate 4.0 Methyl paraben 0.2 incense 0.05 Distilled water Balance Sum 100

Experimental Example  One.

In order to find out the exfoliation effect of the citron extract according to the formulation example 3 of the present invention, the product containing no citron extract in the prepared example 3 is referred to as a control example, and the product containing the citron extract is called as a preparation example. Measured. Experimental method measured the exfoliation effect of the skin by chromaticity change according to the color change of the protein. 15 healthy adults and men and women were identified with clean spots without pigmentation or acne, gently washed with tap water, dried, and then skin-tanned by applying 5% DHA (Dihydroxyacetone) solution twice a day for 2 days. The color of the site colored brown by DHA was measured and compared with the color difference before sample application. After applying the sample twice a day for 2 weeks, the degree of discoloration every day was measured by the skin color measuring instrument (Chromameter, Minolta, Japan) to measure the value of L * was calculated by the exfoliation rate of the following equation 1, the results Table 5 shows.

Equation 1

Keratin exfoliation effect (%) = (measured after X day-DHA) / (measured before DHA-DHA)

Keratin exfoliation effect (%) Experimental Example Keratin exfoliation effect (%) Control Example 16.8 Formulation Example 1 (70% Ethanol Extract of Example 1) 45.8 Formulation Example 2 (purified water extract of Example 2) 40.2 Formulation Example 3 (25% Ethanol Extract of Example 3) 48.6 Formulation Example 4 (50% Ethanol Extract of Example 4) 44.6 Formulation Example 5 (100% Ethanol Extract of Example 5) 46.8 Formulation Example 6 (80% Methanol Extract of Example 6) 43.6 Formulation Example 7 (100% Methanol Extract of Example 7) 44.8 Formulation Example 8 (100% Acetone Extract of Example 8) 42.6 Formulation Example 9 (100% diethyl ether extract of Example 9) 40.2 Formulation Example 10 (100% Hexane Extract of Example 10) 41.3 Formulation Example 11 (100% Normal-Propanol Extract of Example 11) 42.2 Formulation Example 12 (100% Isopropanol Extract of Example 12) 40.6 Formulation Example 13 (100% Normal-Butanol Extract of Example 13) 48.6 Formulation Example 14 (100% Etal Acetate Extract of Example 14) 40.8 Formulation Example 15 (Supercritical Extract of Example 15) 48.7 Formulation Example 3 (Ultrasonic Extract of Example 16) 46.6 Formulation Example 17 (Yeast Fermented Extract of Example 17) 45.2 Formulation Example 18 (Lactic acid bacteria fermentation extract of Example 18) 47.3 Formulation Example 19 (Bacillus fermented extract of Example 18) 46.8 Formulation Example 5 (foam extract of Example 20) 44.5

As can be seen from the results of Table 5, it can be seen that the product containing the citron extract has an excellent skin exfoliation effect.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the same is by way of illustration and example only and is not to be construed as limiting the scope of the present invention. Thus, the substantial scope of the present invention will be defined by the appended claims and equivalents thereof.

Claims (9)

 Cosmetic composition for exfoliation of skin containing citron extract as an active ingredient. According to claim 1, wherein the citron extract is a cosmetic composition, characterized in that the extract of the citron processed by the fermentation method or the preparation method using a microorganism. The cosmetic composition according to claim 2, wherein the microorganism is Saccharomyces cerevisiae as yeast. The method of claim 2, wherein the microorganism is a lactic acid bacterium, Lactobacillus (Lactobacillus), Bifidobacterium (Bifidobacterium), Streptococcus, Streptococcus (Leuconostoc), Pediococcus (Ledococcus) ) And a mixture thereof. According to claim 2, wherein the microorganism is Bacillus (Bacillus sp.), Bacillus subtilis (Bacillus subtilis), Bacillus pumilis (Bacillus pumilis), Bacillus licheniformis (Bacillus licheniformis), Bacillus megaterium (Bacillus megaterium), Bacillus polyfermenticus (Bacillus polyfermenticus), Bacillus mesentericus (Bacillus mesentericus) and a cosmetic composition, characterized in that selected from the group consisting of. The cosmetic composition according to any one of claims 1 to 5, wherein the citron extract contains 0.00001 to 25.0% (v / v) based on the total weight of the cosmetic composition. The citron extract according to any one of claims 1 to 5, wherein the citron extract is water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, propylene glycol, butylene glycol, glycerin, acetone, ethyl acetate, butyl acetate, Cosmetic composition, characterized in that obtained by any one method selected from solvent extraction method, ultrasonic extraction method, supercritical extraction method using an extraction solvent selected from the group consisting of chloroform, diethyl ether, dichloromethane, hexane and mixtures thereof. The cosmetic composition according to any one of claims 1 to 5, wherein the cosmetic composition is a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion Cosmetic composition, characterized in that it has a formulation selected from the group consisting of foundation, wax foundation and spray. The cosmetic composition according to any one of claims 1 to 5, wherein the cosmetic composition is applied to the skin to have a skin exfoliation effect.