KR20130023324A - Cosmetic composition comprising of the mixed herb fermentation extracts - Google Patents

Cosmetic composition comprising of the mixed herb fermentation extracts Download PDF

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Publication number
KR20130023324A
KR20130023324A KR1020130016657A KR20130016657A KR20130023324A KR 20130023324 A KR20130023324 A KR 20130023324A KR 1020130016657 A KR1020130016657 A KR 1020130016657A KR 20130016657 A KR20130016657 A KR 20130016657A KR 20130023324 A KR20130023324 A KR 20130023324A
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South Korea
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cosmetic composition
soybean
mistletoe
fermented extract
present
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KR1020130016657A
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Korean (ko)
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장문식
정욱순
엄선영
조은주
송지은
김동원
김새봄
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장문식
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Publication of KR20130023324A publication Critical patent/KR20130023324A/en

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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783Angiosperms [Magnoliophyta]
    • A61K8/9789Magnoliopsida [dicotyledons]
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783Angiosperms [Magnoliophyta]
    • A61K8/9794Liliopsida [monocotyledons]
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00Preparations for care of the skin
    • A61Q19/007Preparations for dry skin

Abstract

PURPOSE: A cosmetic composition containing a fermented extract of soybeans, Viscum album, and Imperata cylindrical is provided to enhance moisturization and sedation effects. CONSTITUTION: A cosmetic composition contains a fermented extract of soybeans, Viscum album, and Imperata cylindrical. The cosmetic composition has excellent moisturization and sedation effects. The fermented extract is prepared by fermenting a mixture of soybeans, Viscum album, and Imperata cylindrical, filtering, concentrating, and drying. The cosmetic composition is manufactured in the form of a skin softener, a lotion, a nutrition cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, cleansing water, a pack, a spray, or powder.

Description

Highly moisturizing and soothing cosmetic composition comprising a fermented extract consisting of soybean, mistletoe and hair root as an active ingredient {Cosmetic composition comprising of the mixed herb fermentation extracts}

The present invention is a soybean (Soybean), mistletoe ( Viscum Album ), with the root of the hair ( Imperata Cylindrica ) The present invention relates to a cosmetic composition having an excellent moisturizing and soothing effect, comprising the fermented extract as an active ingredient, and more specifically, to the fermentation microorganism Saccharomyces cerevisiae ) or Lactobacillus spp . The present invention relates to a cosmetic composition (trade name: Natural HG or NHG) having excellent moisturizing and soothing effect, containing a fermentation extract obtained by extracting and mixing the first solid fermentation and the second liquid fermentation using).

Patent Document: Korean Application No. 10-2001-0022014

Therefore, the present inventors have tried to find a method to obtain the active ingredient in the highest possible yield than the prior art and to apply it to cosmetics, the method of producing soybean, mistletoe, hair root fermentation extract of the present invention is an antioxidant component It is to provide a cosmetic composition exhibiting abundant concentration of the active ingredient and applying it to cosmetics to exhibit the moisturizing effect, soothing effect, anti-allergic effect and anti-inflammatory effect of the present invention.

The objects and advantages of the present invention will become apparent from the following detailed description and claims.

In order to achieve the above object, the present invention provides a cosmetic composition containing a fermented extract obtained by extracting and filtering the first solid fermentation and the second liquid fermentation of soybean, mistletoe and hair root using yeast or lactic acid bacteria, respectively. .

Preferably, the fermentation is a soybean, mistletoe, hair root is a bacterium selected from the group consisting of yeast, lactic acid bacteria, Bacillus bacteria or mixtures thereof while maintaining a pH of 5 to 7 under temperature conditions of 20 to 40 ℃, 1 to 7 days It provides a cosmetic composition, characterized in that obtained by fermentation.

In addition, the yeast is preferably provided a cosmetic composition, characterized in that Saccharomyces cerevisisae (Saccharomyces cerevisisae).

In addition, the extraction process preferably provides a cosmetic composition, characterized in that obtained by treating for 1 to 24 hours at a temperature of 40 to 90 ℃.

Further, preferably, the extract is a solvent selected from the group consisting of purified water, methanol, ethanol, glycerin, ethyl acetate, butylene glycol, propylene glycol, dichloromethane, chloroform, ethyl ether, butylene glycol, hexane and mixtures thereof. It provides a cosmetic composition, characterized in that the extraction using.

In addition, the soybean, mistletoe, hair root fermentation extract is characterized in that it is contained in an amount of 0.0001 to 30.0% by weight based on the total weight of the cosmetic composition.

In addition, the cosmetic composition preferably provides a cosmetic composition characterized in that it exhibits a moisturizing effect, soothing effect, anti-allergic effect and anti-inflammatory effect.

The cosmetic composition according to the present invention contains active ingredients of soybean, mistletoe and hair root fermentation extract, and has no skin irritation, and has good solubility, affinity, and stability to various bases and additives for cosmetics, organic solvents, and the like. And skin soothing effect was excellent.

The present invention provides a cosmetic composition containing a fermented extract consisting of soybean, mistletoe, hair root obtained by fermenting soybean, mistletoe and hair root, respectively, after extraction and filtration.

According to the invention, the fermented extract consisting of soybean, mistletoe, hair root is excellent in anti-inflammatory effect.

Therefore, the cosmetic composition of the present invention comprising a fermented extract consisting of soybean, mistletoe, hair root having various functions as an active ingredient can be usefully used with excellent anti-allergic effect.

1 is a graph showing the erythema relief effect of the cream containing a fermented extract consisting of soybean, mistletoe, hair roots according to the present invention,
Figure 2 is a graph showing the moisturizing effect over time of the fermented extract consisting of soybean, mistletoe, hair root according to the present invention,
Figure 3 is a graph showing the skin moisture retention rate over time of the fermented extract consisting of soybean, mistletoe, hair root according to the present invention,
Figure 4 is a graph showing the skin soothing and anti-inflammatory effect according to the concentration of the fermented extract consisting of soybean, mistletoe, hair roots according to the present invention,
Figure 5 is a graph showing the anti-inflammatory effect of another form according to the concentration of the fermented extract consisting of soybean, mistletoe, hair roots according to the present invention,
Figure 6 is a graph showing the anti-allergic effect according to the concentration of the fermented extract consisting of soybean, mistletoe, hair roots according to the present invention
Figure 4 is a graph showing the skin soothing and anti-inflammatory effect according to the concentration of the fermented extract consisting of soybean, mistletoe, hair roots according to the present invention,
Figure 5 is a graph showing the anti-inflammatory effect of another form according to the concentration of the fermented extract consisting of soybean, mistletoe, hair roots according to the present invention,
Figure 6 is a graph showing the anti-allergic effect according to the concentration of the fermented extract consisting of soybean, mistletoe, hair roots according to the present invention

The present invention relates to a cosmetic composition having excellent moisturizing and soothing effects, comprising a fermented extract consisting of soybean, mistletoe and hair root obtained by fermenting, extracting and filtering soybean, mistletoe and hair root, respectively. Moisturizing fermentation extract containing fermented extract consisting of soybean, mistletoe and hair root with increased physiologically active substance as fermentation using fermentation microorganism selected from the group consisting of lactic acid bacteria, Bacillus bacteria, and mixtures thereof. A cosmetic composition with excellent soothing effect has been developed.

The cosmetic composition of the present invention as described above is a cosmetic composition with excellent moisturizing and soothing effect, containing a fermentation extract consisting of soybean, mistletoe, hair root obtained by fermenting soybean, mistletoe, hair root and then extracted and filtered.

Fermented extract consisting of soybean, mistletoe, hair root of the present invention composed of the above components is excellent for moisturizing effect and suitable for use as a raw material of the cosmetic composition.

The present inventors extracted and filtered after fermenting soybean, mistletoe and hair root, respectively, in order to find a method to obtain a bioactive substance having various efficacies, especially in the highest yield through a filtration process. It was confirmed that the content of the active ingredient is increased through the process, and applied to the cosmetic to confirm that there is a moisturizing effect to complete the present invention.

Soybean, mistletoe, hair root contained in the cosmetic composition of the present invention is characterized in that consisting of 1 to 50 parts by weight, 1 to 50 parts by weight of mistletoe, 1 to 50 parts by weight of the hair roots based on dry weight and composed of a combination thereof The mixture is a bacterium selected from the group consisting of yeast, lactic acid bacteria, Bacillus bacteria or mixtures thereof, which can be obtained by solid fermentation and liquid fermentation for 1 to 7 days while maintaining pH 5 to 7 under temperature conditions of 20 to 40 ℃. More preferably, it is fermented using Saccharomyces cerevisisae, and most preferably, it is fermented using Saccharomyces cerevisiae KCTC 7904 strain.

Soybean, mistletoe, hair root used in the present invention is used to dry soybean, mistletoe, hair root prepared by a generally known method, they are made of fine powder to pass through 300 mesh size after sufficient washing and dry grinding To primary cultures by primary solid culture at a temperature of 40 to 60 ℃ for 12 to 24 hours. 100 g / L of soybean, mistletoe and hair roots are added to Saccharomyces genus (Saccharomyces cerevisia KCTC 7904) strain culture. 1 to 4% of glucose is used as the carbon source, preferably 1 to 2% is added, 0.1 to 1% of peptone is added, and preferably 0.2 to 0.5% is further added to the second liquid culture. The culture was incubated for 1 to 7 days using a 5L fermenter while maintaining a pH of 5 to 7, under a temperature condition of 20 to 40 ℃. After the culture is characterized in that the culture medium is first removed by centrifugation.

Also preferably, the extraction is performed for 1 to 24 hours at a temperature of 40 to 90 ℃, preferably 12 to 24 hours at a temperature of 40 to 60 ℃, most preferably at a temperature of 50 ℃ Process for 24 hours.

Also preferably, the extraction solvent is selected from the group consisting of purified water, methanol, ethanol, glycerine, ethyl acetate, butylene glycol, propylene glycol, dichloromethane, chloroform, ethyl ether, butylene glycol, hexane and mixtures thereof Extracted using, more preferably extracted using ethanol, 70% aqueous ethanol or water, most preferably 70% aqueous ethanol.

After fermented extract consisting of soybean, mistletoe, and hair root extracted as described above, the mixture was extracted by refluxing three times for 5 hours by adding ethanol to the final 70% (V / V) ethanol aqueous solution, Whatman # 2 Filter with filter paper. When the filtration is completed in the present invention, the extract obtained in the extraction step is transferred to a concentration tank, concentrated under reduced pressure and freeze-dried. Concentration under reduced pressure was concentrated using a rotary vacuum concentrator and concentrated under reduced pressure for 2 hours while putting 700 ml of the extract in a 1,000 ml round flask and rotating at 50 to 70 ° C. and 600 to 1200 rpm at a pressure of 650 mmHg to 750 mmHg. The concentrated solution concentrated under reduced pressure was pre-freezing to lower the temperature to -40 ° C, maintained at -40 ° C for 2 to 4 hours, and then dropped to -80 through trap refrigeration, and the vacuum was maintained at 10-30 mTorr for 120 minutes. Raise back up to -40C. Thereafter, 240 minutes at -20 ° C, 40 minutes at 0 ° C, 340 minutes at 15 ° C, 130 minutes at 30 ° C and 1300 minutes until complete drying.

For reference, the fermented extract consisting of soybean, mistletoe, hair root is an extract obtained by treating the fermented product obtained by fermenting soybean, mistletoe, hair root after ultra high pressure, or the powder is freeze-dried after vacuum, concentration, or extract powder Compounds blended with are preferred.

In addition, the fermented extract consisting of soybean, mistletoe, hair root is not limited to a specific range, but preferably 0.0001 to 30% by weight relative to the total weight of the cosmetic composition. If the content of the extract is less than 0.0001% by weight, the moisturizing effect does not appear, when more than 30% by weight of the effect of increasing the effect of the content is insignificant, there is a problem in the safety and stability of the formulation and is not economical.

Ingredients included in the cosmetic composition of the present invention as an active ingredient includes components conventionally used in cosmetic compositions in addition to the active ingredient, for example, conventional auxiliaries such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavors, And carriers.

Cosmetic compositions of the present invention may be prepared in any formulation conventionally prepared in the art, including, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing, It may be formulated as an oil, powder foundation, emulsion foundation, wax foundation, spray, and the like, but is not limited thereto.

More specifically, it can be manufactured in the form of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder.

When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular in the case of a spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol oil, glycerol aliphatic ester, polyethyleneglycol or sorbitan.

When the formulation of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol esters and polyoxyethylene sorbitan esters, and microcrystals are used as carrier components. Soluble cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, an isethionate, an imidazolinium derivative, a methyltaurate, a sarcosinate, a fatty acid amide ether. Sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

When the cosmetic composition of the present invention is a soap, surfactant-containing cleansing or surfactant-free cleansing formulation, it may be wiped off, peeled off or washed with water after application to the skin. The surfactant-containing cleansing formulation is a cleansing foam, a cleansing water, a cleansing towel, and a cleansing pack. The surfactant-free cleansing formulation may be a cleansing cream, , Cleansing lotion, cleansing water and cleansing gel, but is not limited thereto.

The cosmetic composition of the present invention can be applied to cosmetics that can help to maintain the skin's moisturizing and elasticity to improve the skin's defense in winter.

In addition, the cosmetic composition of the present invention can be applied to cosmetics in anticipation of skin soothing effect and anti-inflammatory effect.

The cosmetic method of the present invention refers to all cosmetic methods using the cosmetic composition of the present invention. That is, all the methods known in the art using the cosmetic composition belong to the makeup method of the present invention.

In addition, the present invention provides a cosmetic method that can obtain a skin moisturizing effect using a cosmetic composition comprising the soybean, mistletoe, hair root fermentation extract.

It refers to all the cosmetic methods for applying the cosmetic composition of the present invention to human skin. That is, all methods known in the art for applying the cosmetic composition to the skin belong to the cosmetic method of the present invention.

The cosmetic composition of the present invention may be used alone or in combination, or may be used by overlapping with other cosmetic compositions other than the present invention. In addition, the cosmetic composition with excellent skin moisturizing effect, soothing effect, anti-allergic effect and anti-inflammatory effect according to the present invention may be used according to a conventional method of use, and the number of times of use may vary depending on the user's skin condition or taste.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood that the scope of the present invention is not limited by these examples in accordance with the gist of the present invention, and it is to be understood by those skilled in the art that the present invention is not limited thereto It will be obvious.

<Example 1> Preparation of fermented extract consisting of soybean, mistletoe, hair root (trade name: Natural HG or NHG)

30 g of soybean, 30 g of mistletoe, and 40 g of hair roots were selected as a sample, thoroughly washed and dried, and finely ground to pass through a 300 mesh sieve, and then subjected to primary solid fermentation with Lactobacillus lactic acid bacteria at a temperature of 40 to 60 ° C for 12 hours. . Saccharomyces genus (Saccharomyces cerevisia KCTC 7904) strain culture solution was added to the primary fermentation at a level of 0.5% based on solids. 1 to 4% of glucose was used as the carbon source, preferably 2% (W / V) was added, and 0.1% of peptone was added to the culture. The culture was incubated for 5 days using a 5L fermenter while maintaining the pH at 5-7 at 37 ° C. After the culture of the microorganism, the culture was centrifuged to remove the culture. Soybean, mistletoe and hair roots from which the cultures were first removed were heated to 50 ° C. and subjected to ultra high pressure for 24 hours, followed by cooling to room temperature. Secondary fermented product consisting of ultra-high pressure soybean, mistletoe and hair roots was refluxed and refrigerated three times for 5 hours by adding ethanol to the final 70% (V / V) ethanol aqueous solution, and then Whatman # 2 Filter by filter paper. When the filtration is completed in the present invention, the extract obtained in the extraction step was transferred to a concentration tank and concentrated under reduced pressure at 50 ℃ or less and then freeze-dried. The lyophilisate was dissolved in 2% using 50% glycerin to prepare 20 g of fermented extract (Example 1) consisting of soybean, mistletoe and hair root.

Example 2 Preparation of a Nourishing Cream Containing Fermented Extract Composed of Soybean, Mistletoe, and Hair Roots According to the Present Invention

Dissolving the purified water portion by heating it to 80 ° C., emulsifying by adding the dissolved oily part heated to 80 ° C., adding and mixing a thickener dissolved in warm water at 60 ° C., and cooling and adding and mixing cosmetic ingredients. Cooling to 30 ℃ and defoaming, performing the step of filtration and aging prepared at 50 ℃ to prepare a nutrient cream as shown in Table 1.

Classification Raw material name Weight (% by weight) Purified water Purified water 60.36 Propylene glycol 5.0 glycerin 6.0 Xanthan gum 0.1 Triethanolamine 0.7 Methylparaben 0.2 Phenoxyethanol 0.1 Fermented extract of the present invention 2.0 Oil component Squalane 8.0 Cetyl alcohol 2.2 Cyclopentasiloxane 2.0 Stearic acid 1.5 Glyceryl monostearate 1.0 Microcrystalline Wax 0.6 Fiji-100 0.3 Tocopherol Acetate 1.0 Shea butter 1.0 Propylparaben 0.1 Oryzanol 0.1 Bisabolol 0.2 Dimethicone 0.5 Monostearic acid polyoxyethylene sorbitan 0.8 Thickener Carbomer 0.24 Purified water 2.4 Beauty ingredients Sodium ascorbate 0.1 Phospholipids 1.0 Placenta Extract 0.5 Aloe extract 0.5 Tocopherol acetate 0.5 Aloe extract 1.0 Sum 100.00

<Experiment 1> Confirm the skin irritation and protective effect of the nutrient cream containing fermented extract consisting of soybean, mistletoe, hair root according to the present invention

Apply the nutrient cream prepared in Example 3 (10m / 5cm 2 ) to the forearm of 40 healthy men and women (20 males and 20 females), and cover the rest with aluminum foil for 30 minutes. Subsequently, a long wavelength ultraviolet ray was irradiated at a dose of 10.2 J / cm 2 using an ultraviolet irradiator, and skin conditions (erythema, edema, papules, etc.) were observed 24 hours later. As a result of observation, no side effects such as erythema were generated on the skin, and thus the nourishing cream had no phototoxicity and had a protective effect on the skin. In addition, a group of 40 healthy men and women (20 men, 20 women) divided into two groups, the experimental group and the soybean, mistletoe according to the present invention and the experimental group to apply a certain amount (10m / 5cm 2 ) of the nutrition cream prepared in Example 2 , Example 2 was prepared without containing the fermented extract consisting of the hair root as a control and after 1 hour, 3 hours, 6 hours, the skin elasticity is a hollow cylinder consisting of a 10mm diameter border to give a force of about 5N to disappear marks As a result of comparing the time, it is disappeared from the experimental group at about 17% level in each hour compared to the control group, and the skin elasticity is improved by using the nourishing cream containing fermented extract composed of soybean, mistletoe and hair root of the present invention. It could be confirmed.

Experimental Example 2 Test for confirming calming effect of nourishing cream containing fermented extract consisting of soybean, mistletoe and hair root according to the present invention

A human patch test was performed on the nourishing cream prepared in Example 2 on the forearm of 40 healthy adult men and women (20 males, 20 females). After wiping the forearm of the subject well with 70% ethanol, the pin chamber containing 20 µl of the nourishing cream prepared in Example 2 in a common cosmetic base was closed and attached to the site 24 hours using an adhesive tape. After 24 hours, the adhesive tape and the pin chamber were removed, and the sample remaining on the skin with a gauze was lightly removed, and then the skin condition was observed, and again after 24 hours. As a result of the experiment, as shown in Figure 1 in the human patch test to confirm the skin irritation of the nourishing cream containing a fermented extract consisting of soybean, mistletoe, hair roots according to the present invention did not show a positive response to the human skin at all It was confirmed.

Experimental Example 3 Experiment for Measuring Moisturizing Effect of Fermented Extract Composed of Soybean, Mistletoe and Hair Roots According to the Present Invention

In the same manner as in Experiment 3, a sample of a control group of soybean, mistletoe, hair root fermentation extract and a comparative experiment group containing 0.2% of hyaluronic acid was used. In general, the evaluation method, which is called the hydration degree of the stratum corneum, that is, the skin moisture content, is mainly evaluated using the electrical properties of the stratum corneum. The most commonly used method of evaluating water content is by capacitance. Healthy subjects are selected and cosmetics are applied to the forearm to evaluate the capacitance over time. In this experiment, the skin capacitance was measured using the moisture checker MY-808S (Scalar, Tokyo), and the results were expressed as moisture content (%). 2, the moisture content (%) of the control group (sample 1) and hyaluronic acid using a 0.2% hyaluronic acid compared with the time of the fermentation extract consisting of soybean, mistletoe, hair roots according to the present invention (sample 2) was confirmed that the relative increase in water content (%) It was. In the case of the fermented extract consisting of soybean, mistletoe, hair root of the present invention showed a difference in water retention nearly two times after 1 hour immediately after application compared to the control group, the water content is maintained constant up to 3 hours after the experiment It was found that the moisturizing effect of the cosmetic composition containing the fermentation extract consisting of soybean, mistletoe, hair root of the present invention is excellent.

In addition, after applying the natural HG to the forearm of the fermentation extract consisting of soybean, mistletoe, hair root according to the present invention in FIG. 3 after 2, 4, 6, 8 hours, the skin moisture retention rate was measured, to confirm a significant difference Could.

Experimental Example 4 Experiment of Determination of Skin Soothing and Anti-Inflammatory Effects According to Concentration of Soybean, Mistletoe and Hair Root Fermentation Extracts According to the Present Invention

In the same manner as in Experiment 3, soybean, mistletoe, and the control group and the sample of the experimental group of the hair root fermentation extract were used. Various mechanisms are involved in the mechanism of expression of inflammation, and the causes of the disease also vary. Treatment of lipopolysacharide (LPS), known as endotoxin, in macrophages increases NO biosynthesis and induces inflammatory responses by iNOS (inducible NOS) expression. NO is a pathological secondary signaling agent secreted by many cells and regulates COX activity and acts synergistically in the inflammatory response. The NO produced by LPS-induced NOS in Raw264.7 cells can be measured to determine the inhibitory effects of fermented extracts consisting of soybean, mistletoe and hair roots according to the present invention. In Figure 4 it was confirmed that the skin sedation effect is increased by about 23% compared to the control group according to the concentration of the fermented extract consisting of soybean, mistletoe, hair root according to the present invention.

In addition, lipoxygenase (5-LO, 5-lipoxygenase) adds molecular oxygen to unsaturated fatty acids having 1,4- cis and cis- pentadiene (1,4-pentadiene) structures in the molecule, such as linoleic acid, linolenic acid, and arachidonic acid. It is an oxidase that produces 1,3- cis and trans -diene 5-hydroperoxide. It is an enzyme involved in the first reaction of leucotriene biosynthesis from arachidonic acid, which is involved in various inflammatory and allergic diseases. Therefore, by measuring the inhibitory activity of lipoxygenase using SLO (soybean lipoxygenase) similar to 5-LO structurally and mechanically, the fermentation extract consisting of soybean, mistletoe and hair roots according to the present invention has an anti-inflammatory effect. It confirmed that it was excellent.

<Experiment 5> Anti-allergic effect measurement experiment according to the concentration of soybean, mistletoe, hair root fermentation extract according to the present invention

In the same manner as in Experiment 3, soybean, mistletoe, and the control group and the sample of the experimental group of the hair root fermentation extract were used. Chemical allergens such as Histamine may cause characteristic reactions that increase vascular permeability, dilate blood vessels, contract smooth muscles, and increase the function of the secretory glands. Histamine and -hexosaminidase act as an important mediator of allergy and the release of -hexosaminidase from cells is also used as an indicator of immune cell degranulation. In Figure 6, according to the concentration of the fermented extract consisting of soybean, mistletoe, hair roots according to the present invention, the anti-allergic effect was confirmed that the skin soothing effect increased about 25% compared to the control.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, but on the contrary, &Lt; RTI ID = 0.0 &gt; and / or &lt; / RTI &gt;

Claims (7)

Cosmetic composition containing fermented extract consisting of soybean, mistletoe and hair root The cosmetic composition of claim 1, wherein the fermented extract is manufactured by filtering, concentrating, and drying the soybean, mistletoe, and hair root mixture through a fermentation step. The cosmetic composition according to claim 1, wherein the fermented extract is contained in an amount of 0.0001 to 30.0% by weight in dry weight. The cosmetic composition of claim 1, wherein the cosmetic composition including the fermented extract has a flexible lotion, a nourishing lotion, a nourishing cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray, or a powder formulation. Cosmetic composition The cosmetic composition according to claim 1, wherein the fermented extract has a skin moisturizing effect.
The cosmetic composition according to claim 1, wherein the fermented extract has a soothing and anti-inflammatory effect. The cosmetic composition according to claim 1, wherein the fermented extract has an antiallergic effect.
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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101634670B1 (en) 2015-07-27 2016-06-30 강원대학교산학협력단 Cosmetic composition comprising fermented extract of mistletoe for anti-inflammation or anti-allergy
CN106619379A (en) * 2016-12-27 2017-05-10 广东芭薇生物科技股份有限公司 Composition containing plant fermentation product as well as preparation method and application of composition
KR20210001935A (en) 2019-06-28 2021-01-06 강원대학교산학협력단 Anti-allergy composition comprising mistletoe extract or fraction

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101634670B1 (en) 2015-07-27 2016-06-30 강원대학교산학협력단 Cosmetic composition comprising fermented extract of mistletoe for anti-inflammation or anti-allergy
CN106619379A (en) * 2016-12-27 2017-05-10 广东芭薇生物科技股份有限公司 Composition containing plant fermentation product as well as preparation method and application of composition
CN106619379B (en) * 2016-12-27 2018-04-06 广东芭薇生物科技股份有限公司 A kind of composition of the product containing plant fermentation and its preparation method and application
KR20210001935A (en) 2019-06-28 2021-01-06 강원대학교산학협력단 Anti-allergy composition comprising mistletoe extract or fraction

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