KR20190049067A - A cosmetic composition comprising extract of coffee silver skin and coffee grounds - Google Patents

Abstract

The present invention relates to a cosmetic composition containing an extract of coffee silver skin and coffee waste as an active ingredient, which has an excellent skin improvement effect. The extract is a fermented extract prepared by inoculating a fermentation strain.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a cosmetic composition containing a coffee silver skin and coffee bean extract as an active ingredient,

The present invention relates to a cosmetic composition comprising a coffee silver skin and a coffee bean extract as an active ingredient.

The skin is largely divided into epidermis, dermis and subcutaneous fat, and plays an important role in protecting the body from physical, chemical, or biological stimulation of the human body.

In addition, the skin protects the internal organs of the skin from harmful ultraviolet rays from the outside, plays an important role in maintaining the homeostasis of the body, such as suppressing the evaporation of moisture in the skin and controlling the body temperature.

The epidermis, which is the outermost layer of the skin, protects the human body from the outside, and the stratum corneum, which is the uppermost layer of the epidermis, is an epidermal barrier. It prevents evaporation of moisture and loss of epidermis, prevention of drying of the epidermis, and prevention of bacterial, fungal, Of the skin. Melanocytes, one of the skin's epidermal cells, play a role in protecting the skin from ultraviolet rays by generating melanin.

However, as the age increases, the secretion of various hormones that regulate the metabolism inside the skin decreases, and when the functions of the immune cells and the activity of the skin cells are lowered, various troubles such as inflammation and atopy occur in the skin. In addition, when ultraviolet rays penetrate into the skin due to a decrease in function of the skin, free radicals and active harmful oxygen are increased and melanin is deposited on the cells, and skin is damaged by spots, freckles and wrinkles.

Various external preparations for skin have been developed to prevent such skin troubles and skin damage. However, since general skin external preparations contain chemicals, they are irritating to the skin and have side effects such as erythema and redness in skin application. Further, the effect is insignificant, and it is difficult to expect an effect of substantially improving the skin, and there arises a problem of causing skin troubles such as acne and atopy during long-term use.

Accordingly, the cosmetics industry is developing a number of products using natural products to reduce skin irritation caused by various chemicals. Physiologically active substances obtained from natural products maintain skin's natural function, activate skin cells to maintain skin health, and have high stability, so that the development of cosmetics including natural products is increasing.

On the other hand, the value of byproducts is increasing as recent industrial byproducts are reexamined to other fields. In particular, coffee silver skin is a solid by-product of processing coffee beans, and recent studies have reported that coffee silver skins contain a variety of antioxidants (Francisca Rodrigues et al., 2014, Ana Jimenez-Zamora et al., 2015).

The coffee silver skin is a thin silver film wrapped around a coffee bean, which is naturally peeled off during the roasting process. Generally, the coffee silver skin produced in the coffee process is discarded as waste or part of it is used as fertilizer (Saenger et al., 2001) because if the coffee silver skin is not properly removed from the bean, the quality of the coffee taste deteriorates.

Because coffee silver skin contains antioxidants and is a by-product of the coffee processing process, coffee silver skin has attracted attention as a source of industrially available antioxidants. However, coffee silver skin has not yet been directly applied to cosmetics Have not been reported.

On the other hand, the coffee residue, which is extracted from coffee beans, is a large amount of wastes generated by the world over 6 million tons per year. However, there is a lack of utilization and treatment methods. Therefore, there is a growing interest in the recycling of coffee beans, and research has been conducted on the use of coffee beans as fuel, feed, or fertilizer additives, or as substrates for producing mushrooms and as raw materials for biodiesel.

As a result of careful examination of the possibility and application of coffee byproducts, the inventors of the present invention have confirmed that the effect of improving the skin can be remarkably increased by applying coffee silver skin and coffee bean extract, and utilized this as a material of functional cosmetics.

Disclosure of Invention Technical Problem [8] Accordingly, the present invention has been made keeping in mind the above problems occurring in the prior art, and an object of the present invention is to provide a cosmetic composition having excellent functionality including a coffee by-product extract.

According to one aspect of the present invention, there is provided a cosmetic composition comprising a coffee silver skin and coffee bean extract as an active ingredient.

In one embodiment, the extract may be a fermentation extract prepared by inoculating a fermentation strain.

In one embodiment, the fermentation strain is selected from the group consisting of Hericium erinaceum , Pleurotusus ferulae), Blossom Mushrooms (Sparassis crispa , Ganoderma lucidum , Flammulina velutipes , Phellinus linteus , and Grifola frondosa .

In one embodiment, the composition may further comprise one or more selected from the group consisting of coffee bean oil, coffee bean extract, coffee bean powder, coffee tree leaf extract, coffee tree fruit extract and coffee tree bud extract.

In one embodiment, the composition may further comprise at least one member selected from the group consisting of green tea extract, oolong tea extract, vide tea extract, black tea extract, and black tea extract.

In one embodiment, the composition may further comprise a complex extract consisting of a camellia flower extract, a chamomile flower extract, and a lotus flower extract.

In one embodiment, the composition may be anti-aging.

In one embodiment, the composition may be for skin elasticity and wrinkle improvement.

In one embodiment, the composition is selected from the group consisting of softening longevity, nutritional lotion, moisture cream, nutritional cream, massage cream, nutritional lotion, essence, ampoule, gel, eye cream, oil, foundation, wax, cleansing cream, cleansing foam, , Shampoos, rinses, packs, hair creams, hair waxes, hair gels, sprays, and powders.

Since the cosmetic composition according to one aspect of the present invention includes coffee silver skin and coffee ground extract, it can be used not only for sensitive skin but also for improving skin such as heat aging and wrinkle reducing effect.

It should be understood that the effects of the present invention are not limited to the effects described above, but include all effects that can be deduced from the description of the invention or the composition of the invention set forth in the claims.

As used herein, the terminology used herein is intended to encompass all commonly used generic terms that may be considered while considering the functionality of the present invention, but this may vary depending upon the intent or circumstance of the skilled artisan, the emergence of new technology, and the like. Also, in certain cases, there may be a term selected arbitrarily by the applicant, in which case the meaning thereof will be described in detail in the description of the corresponding invention. Therefore, the term used in the present invention should be defined based on the meaning of the term, not on the name of a simple term, but on the entire contents of the present invention.

Unless defined otherwise, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Terms such as those defined in commonly used dictionaries are to be interpreted as having a meaning consistent with the contextual meaning of the related art and are to be interpreted as either ideal or overly formal in the sense of the present application Do not.

The numerical range includes numerical values defined in the above range. All numerical limitations of all the maximum numerical values given throughout this specification include all lower numerical limitations as the lower numerical limitations are explicitly stated. All the minimum numerical limitations given throughout this specification include all higher numerical limitations as the higher numerical limitations are explicitly stated. All numerical limitations given throughout this specification will include any better numerical range within a broader numerical range, as narrower numerical limitations are explicitly stated.

Hereinafter, embodiments of the present invention will be described in detail, but it should be apparent that the present invention is not limited by the following examples.

According to one aspect of the present invention, there is provided a cosmetic composition comprising a coffee silver skin and coffee bean extract as an active ingredient.

The cosmetic composition of the present invention contains an extract of natural origin as an active ingredient, so that it is excellent in skin improving effect and biosafety, and skin irritation can be minimized.

The "coffee silver skin" is called silverskin and is a silver cuticle that surrounds the center cut of the coffee bean and the green bean, the green bean. The coffee beans are usually called beans, but are seeds of coffee trees. The coffee tree has red berries similar to cherries. When the fruit is peeled off, it has a hard skin pachment. When the peach is peeled off, it has a keratinized silver skin and coffee bean. The parchment and silver skin generated during processing of coffee beans are discarded without being used.

According to a recent study, the above coffee silver skin has high antioxidant ability due to its high content of flavonoids. In the stability test, there is no cytotoxicity to fibroblasts and keratinocytes. However, there is no toxicity to harmful bacteria such as K. pneumoniae , S. epidermidis and S. aureus (Francisca Rodrigues et al., 2015).

It is estimated that the above-mentioned "coffee bean" is an extraction residue produced in the process of producing coffee hot-water extract, and is produced over 6 million tons / year worldwide. (Saenger, M., Hartge, E. Werther, J. Ogada, T. and Siagi, Z. 2001. Combustion of coffee husks. Renew. Energ. 23: 103- 121.), a functional material source (Simoes, J., Madureira, P., Nunes, FM, Domingues, MR Vilanova, M. and Coimbra, MA 2009. Immunostimulatory properties of coffee mannans. : 1036-1043.), A biodiesel feedstock (Franca, AS, Oliveira, LS and Ferreira, ME 2009. Kinetics and equilibrium studies of methylene blue adsorption by spent coffee grounds. Desalination 249: 267-272. ought.

As used herein, the phrase " comprising as an active ingredient " may mean an effective amount of the composition capable of exhibiting a skin improving effect, for example, exhibiting collagen synthesis, elasticity improvement or antioxidative effect related to a wrinkle reducing effect.

The coffee silver skin and coffee bean contain various physiologically active ingredients. When the extract is applied to the skin at the same time, the skin improving effect can be remarkably increased due to the synergistic effect.

The " extract " is obtained by separating an active ingredient contained in an extractive raw material by contacting the solvent and an extractive raw material under specific conditions. If the ingredient contained in the raw material is separated from a natural product, can do. For example, it may include extracts of components dissolved in a solvent from natural products by using water or an organic solvent, and specific components of natural products, such as oils obtained by extracting only specific components such as oil.

The extract can be obtained by drying the coffee silver skin and the coffee bean followed by pulverization, or various extraction methods known in the art such as hot water extraction and ethanol extraction.

Wherein the extract is selected from the group consisting of water, an anhydrous or lower alcohol having 1 to 4 carbon atoms, acetone, petroleum ether, ethyl acetate, butyl acetate, trichloromethane, dichloromethane, chloroform, hexane and 1,3-butylene glycol , And may be extracted with ethanol, preferably.

The extraction ratio of the active ingredient contained in the raw material may be different depending on the polarity of the solvent. Since the ethanol is excellent in selectivity for extracting a physiologically active substance from a natural raw material, the ethanol extract can realize an optimal skin improving effect .

The extract is washed with water, dried and pulverized, and then extracted and extracted with a solvent having a volume of 10 to 20 times the weight of the raw material for about 24 to 240 hours by a conventional method such as circulation extraction, pressure extraction, Followed by filtration. In addition, the extract can be obtained in powder form by an additional process such as vacuum distillation or freeze-drying.

The extract may also contain an extract which has been subjected to a conventional purification process. For example, the extract may be subjected to various purification methods such as separation using an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity) Lt; RTI ID = 0.0 > fractions. ≪ / RTI >

The extract may be a fermentation extract prepared by inoculating a fermentation strain.

The term " fermentation " refers to a process of decomposing an organic substance using an enzyme possessed by the fermentation strain itself. It is known that raw materials after fermentation through fermentation strains are at least two times to several tens of times more effective than before fermentation. Fermented metabolites contain various amino acids, organic acids, and antioxidants that are good for skin, promoting skin metabolism and smooth skin texture. In addition, as the fermentation process proceeds, the particles become smaller and the toxicity such as heavy metals is decomposed, so that the absorption rate is good, and skin troubles and allergy side effects can be alleviated.

The "fermentation extract" may be prepared by sterilizing the coffee silver skin and the coffee bean, inoculating the fermentation strain, fermenting the fermented coffee silver skin at a predetermined temperature for a certain period of time, and extracting the fermented coffee silver skin with a specific solvent, preferably ethanol have.

The fermentation strains were obtained from Hericium < RTI ID = 0.0 > erinaceum , Pleurotusus ferulae , Sparassis crispa , Ganoderma lucidum , Flammulina velutipes ), mushroom ( Phellinus linteus and Grifola frondosa , but the present invention is not limited thereto.

The composition may further comprise at least one selected from the group consisting of coffee bean oil, coffee bean extract, coffee bean powder, coffee tree leaf extract, coffee tree fruit extract and coffee tree bud extract.

The composition may further enhance the skin-improving effect when applied with coffee silver skins and coffee bean extracts, as well as with coffee-derived extracts such as coffee leaves, berries, sprouts, coffee bean oil or extracts.

The composition may further include at least one selected from the group consisting of green tea extract, oolong tea extract, vide tea extract, black tea extract, and black tea extract.

The tea-derived extract contains various antioxidants such as polyphenol (TPP, tea polyphenol), carotenoid, vitamin E and vitamin C, and can be used on the market as needed.

The cosmetic composition may further comprise a complex extract composed of a camellia flower extract, a chamomile flower extract, and a lotus flower extract.

The " Camellia japonica " is an evergreen broad-leaved arboreous tree. The stem of the camellia is white with many twigs and has an elliptical or hemispherical shape. The Camellia sinensis has five petals, purplish red or white, and from November to March of the following year, and the seedlings are mature at the end of October. The major components of the camellia are tannin, camelliin and leucocyanidin, and the seed has 66% oil and its main component is oleic acid, glyceride.

Camellia (C18H34O7) and camellia saponin (C58H92O25) are present in the seeds of Camellia sinensis (Encyclopedia of Science, Publications, 1999). Camellia is known to have a radiant action, Camellia saponin is known to have a hemolytic action, and Camellia oil is used as an oil-based ointment.

The Camellia sinensis is a red blood flower used as a hematopoietic agent for blood circulation and vaginal bleeding in the private sector. It is used as a powder in the form of urine, ginger juice and alcohol, and antigens and antinociceptors (Namba et al., Shoyakugaku Zasshi, 38 3, pp. 263-264, 1984), inhibition of tartar formation (Yoshikawa et al., Chem. Pharm. Bull. Physiological activity such as whitening action (Sakata et al., Mem. Fac. Agr. Kagoshima Univ., 17, pp. 79-94, 1981) was reported in Patent Document 63-303910, have.

The "Chamomile (Chamomilla recutita ) "is a grass belonging to the dicotyledonous plant. Chamomile is sedative and helps relieve all kinds of pain. Chamomile flower and leaf extracts calm and soften reddened skin. In addition, the above-mentioned chamomile is widely used as a bath agent in Europe because of its high sterilizing effect and systemic cosmetic effect, and is effective for the poor circulation of women, and has the effect of alleviating low saltiness, preservative, anthelmintic and cramps.

The "lotus ( Nelumbo nucifera ) "is a plant that has many years of water life. It is native to southern Asia and northern Australia and has large pink or white flowers in summer. The lotus has various effects such as nourishment, calm convergence, and branches, and its efficacy is used to treat leftover diseases such as gastroenteritis and indigestion insomnia. The lotus extract has a powerful antioxidant effect and soothing effect on the skin, and the lotus germination water has a moisturizing effect that replenishes moisture to the skin and alleviates the texture of the skin.

The camphorol component contained in the lotus is a type of flavonoid, and has excellent effect of preventing skin damage due to oxidation. It inhibits the production of active oxygen and active nitrogen, which are the cause of aging, and protects skin by removing it. It contains anti-aging and wrinkle improving effect because it contains a large amount of vitamin C which helps collagen synthesis. The lotus flower contains enriched linoleic acid, which is an unsaturated fatty acid, so that moisture loss of dry skin is suppressed, so that the moisturizing effect is excellent.

In one embodiment, the extract can be extracted for 2 to 18 hours at a temperature of 50 ° C to 90 ° C using the ethanol as a solvent.

If the extraction temperature is lower than 50 캜, the extraction efficiency may be lowered and the active ingredient may not be eluted properly. If the extraction temperature is higher than 90 캜, the active ingredient contained in the natural raw material may be deformed or lost at high temperature, It is preferable to keep the extraction temperature within an appropriate range. If the extraction time is less than 2 hours, the active ingredient contained in the natural raw material may not be completely extracted. If the extraction time exceeds 18 hours, the content of the remaining active ingredient contained in the natural raw material is inadequate in terms of time and cost It is desirable to appropriately control the extraction time.

The content of the coffee silver skin coffee bean extract may be 0.001% by weight to 30.0% by weight based on the total weight of the cosmetic composition. If the content of the coffee silver skin is less than 0.001% by weight, the skin improving effect of the active ingredient may not be sufficiently realized. If the content is more than 30% by weight, the specific gravity of the other ingredients required for commercialization may decrease, , The cost efficiency may be lowered.

The cosmetic composition may be used for prevention of heat aging, skin elasticity and wrinkles.

The " improvement of skin elasticity " means that elastic fibers composed of elastin are present together with collagen, and elastic elasticity is maintained or improved in a state where elastin and collagen are sufficiently present.

Further, the skin elasticity can be improved and the effect of improving the skin wrinkles can be accompanied. The " wrinkle improvement " means a phenomenon that inhibits or inhibits the generation of wrinkles on the skin, or alleviates the already formed wrinkles.

The above-mentioned " thermal aging " is a phenomenon in which skin is aged by infrared rays in addition to ultraviolet rays, and thermal skin aging accelerates photoaging, and a number of experimental results (J. Dermatological Sci. Supplement (2006) S22 " Thermal aging: a new concept of skin aging ", Photodermatol Photoimmunol Photomed (2006) 22, 148-152, " Minimal heating dose: a novel biological unit to measure infrared irradiation " The cosmetic composition can effectively suppress skin temperature rise and heat aging phenomenon caused by infrared rays, and can be utilized as an application for prevention of heat aging.

The cosmetic composition of the present invention can be used as a cosmetic composition for cosmetics such as soft longevity, nutrition lotion, water cream, nutritional cream, massage cream, nutrition lotion, essence, ampoule, gel, eye cream, oil, foundation, wax, cleansing cream, cleansing foam, And may be formulated into one or more selected from the group consisting of a pack, a hair cream, a hair wax, a hair gel, a spray and a powder.

The cosmetic composition may be formulated by a conventional method. The contents disclosed in Remington ' s Pharmaceutical Sciences, Mack Publishing Company, Easton PA in the formulation of the external preparation for skin can be referred to, and in the formulation of the cosmetic composition, International cosmetic ingredient dictionary, 6th ed (The cosmetic, Toiletry and Fragrance Association , Inc., Washington, 1995).

The above-mentioned cosmetic composition can be prepared into a general emulsified formulation and a solubilized formulation. For example, lotion such as soft lotion or nutrition lotion; Lotion such as facial lotion, body lotion; Nourishing cream, moisture cream, cream such as eye cream; essence; Makeup ointment; spray; Gel; pack; Sunscreen; Makeup base; A foundation such as a liquid type, a solid type or a spray type; powder; Make-up removers such as cleansing creams, cleansing lotions and cleansing oils; Or a cleansing agent such as a cleansing foam, a soap, a body wash, and the like, but is not limited thereto. The external preparation for skin may be formulated into ointments, patches, gels, creams or sprays, but is not limited thereto.

The cosmetic composition of the present invention may be appropriately blended with other ingredients within the range of not compromising the object of the present invention depending on the kind of the formulation or the purpose of use, in addition to the essential ingredients in each formulation.

The cosmetic composition may contain a conventional acceptable carrier and may appropriately contain, for example, oil, water, a surfactant, a moisturizer, a lower alcohol, a thickener, a chelating agent, a colorant, no.

The acceptable carrier may vary according to the formulation. For example, when formulated into ointments, pastes, creams or gels, there may be used an animal oil, a vegetable oil, a wax, a paraffin, a starch, a tracer, a cellulose derivative, polyethylene glycol, silicon, bentonite, silica, talc, zinc oxide, Mixture can be used

When the cosmetic composition is formulated into a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder or a mixture thereof may be used as a carrier component. In the case of a spray, Propellants such as carbon, propane, butane or dimethyl ether.

When the cosmetic composition is formulated into a solution or emulsion, a solvent, a solubilizing agent or an emulsifying agent may be used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl benzoate, 1,3-butyl glycol oil may be used, and in particular, fatty acid esters of cottonseed oil, peanut oil, corn oil, olive oil, castor oil and sesame oil, glycerol aliphatic ester, polyethylene glycol or sorbitan may be used.

When formulated into a suspension, the cosmetic composition may contain, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the cosmetic composition is formulated into soap, it is preferable to use, as a carrier component, an alkali metal salt of a fatty acid, a fatty acid hemiester salt, a fatty acid protein hydrolizate, isethionate, a lanolin derivative, an aliphatic alcohol, a vegetable oil, Can be used.

The cosmetic composition may be a cosmetic or dermatological composition which is generally used in the art depending on the quality or function of the final product, such as a lipid, an organic solvent, a solubilizing agent, a thickening agent, a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizer, Surfactants, water, ionic or nonionic emulsifiers, fillers, metal ion sequestrants, chelating agents, preservatives, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, And may further contain adjuvants conventionally used in the field of cosmetics or dermatology, such as any other ingredient.

However, the adjuvant and the mixing ratio thereof can be appropriately selected so as not to affect the preferable properties of the cosmetic composition according to the present invention.

The present invention will be further described with reference to the following examples, but it should be apparent that the present invention is not limited by the following examples.

Manufacturing example  1: coffee Silver skin  And Coffee shop  Preparation of extract

The coffee silver skin was washed with water, completely dried at room temperature, and pulverized to obtain 100 g of pulverized product. 100 g of ground coffee silver powder was immersed in a 20-fold volume of ethanol as a solvent for 7 days at room temperature

The extract was filtered through a 250 mesh filter and a 0.5 탆 filter. The remaining raw material was repeatedly extracted three times in the same manner, and concentrated under reduced pressure at 50 캜 and freeze-dried to obtain a solid content of coffee silver skin extract.

Manufacturing example  2: Coffee Silver skin  Preparation of fermented extract

1 to 4% of glucose was added as a carbon source to 5 g of the coffee silver skin concentrated concentrate prepared in Preparation Example 1, 0.2 to 0.5% of peptone was further added, and sterilized. At a rate of 25g / L, Sparassis crispa ) were inoculated and cultured. 5 L fermenter for 7 days at 37 ° C and pH 5-7. After culturing, the culture broth was centrifuged to remove the culture medium first, and sterilized (121 ° C, 15 minutes, 1.5 atm) to prevent further culture.

After the fermentation, the fermented coffee silver skin, from which the culture was firstly removed, was subjected to reflux extraction three times for 5 hours by adding ethanol to make a final 70% (v / v) ethanol aqueous solution, Filtered through filter paper. Upon completion of the filtration, the extract obtained in the extraction step was transferred to a concentration tank and concentrated under reduced pressure or freeze-dried at 50 DEG C or lower to obtain a solid content of the coffee silver skin fermentation extract.

Manufacturing example  3: Coffee Silver skin  Preparation of fermented extract

The fermented extract was prepared in the same manner as in Preparation Example 2 except that only the fermentation strain was changed to Hericium erinaceum .

Manufacturing example  4: Coffee Silver skin  Preparation of fermented extract

A fermented extract was prepared in the same manner as in Preparation Example 2, except that the fermentation strain was changed to Pleurotusus ferulae .

Manufacturing example  5: Coffee Silver skin  Preparation of fermented extract

The fermented extract was prepared in the same manner as in Preparation Example 2 except that only the fermentation strain was changed to Ganoderma lucidum .

Manufacturing example  6: Coffee Silver skin  Preparation of fermented extract

The fermented extract was prepared in the same manner as in Preparation Example 2 except that only the fermentation strain was changed to Flammulina velutipes .

Manufacturing example  7: Coffee Silver skin  Preparation of fermented extract

The fermented extract was prepared in the same manner as in Preparation Example 2 except that the fermentation strain was changed to Phellinus linteus .

Manufacturing example  8: Coffee Silver skin  Preparation of fermented extract

A fermented extract was prepared in the same manner as in Preparation Example 2 except that only the fermentation strain was changed to Grifola frondosa .

Manufacturing example  9: Preparation of flower complex extract

Camellia flower, Chamomile flower, and lotus flower were washed with water, completely dried at room temperature and pulverized to obtain 100 g each of the pulverized material, and they were mixed to obtain an extraction raw material of the flower composite extract.

Extraction was carried out with a 20-fold volume of ethanol as a solvent at a temperature of 50 DEG C for 7 days.

The extract was filtered through a 250 mesh filter and a 0.5 탆 filter. The remaining ingredients were repeatedly extracted three times in the same manner, concentrated at 50 캜 under reduced pressure, and lyophilized to obtain a solid component of the flower composite extract.

Comparative Manufacturing Example  : coffee Silver skin  Preparation of fermented extract

A fermentation extract was prepared in the same manner as in Preparation Example 2 except that only the fermentation strain was changed to Lactobacillus plantarum .

Example  And Comparative Example

In order to verify the skin improvement effect of the obtained samples, the examples and comparative examples were set by mixing the samples as shown in Tables 1 and 2 below.

[Content (parts by weight)] division Example One 2 3 4 5 6 7 8 10 11 12 13 14 15 16 Production Example 1 60 - - - - - - - - - - - - - - Production Example 2 - 60 - - - - - - 30 30 30 30 30 30 30 Production Example 3 - - 60 - - - - - - - - - - - - Production Example 4 - - - 60 - - - - - - - - - - - Production Example 5 - - - - 60 - - - - - - - - - - Production Example 6 - - - - - 60 - - - - - - - - - Production Example 7 - - - - - - 60 - - - - - - - - Production Example 8 - - - - - - - 60 - - - - - - - Coffee bean oil - - - - - - - - 30 - - - - - 5 Coffee bean extract - - - - - - - - 30 - - - - 5 Coffee tree leaf extract - - - - - - - - - - 30 - - - 5 Green tea extract - - - - - - - - - - - 30 - - 5 Oolong tea extract - - - - - - - - - - - - 30 - 5 Production Example 9 - - - - - - - - - - - - 30 5

[Content (parts by weight)] division Comparative Example One 2 3 4 5 6 7 8 9  Comparative Manufacturing Example - - - - - - - - 60 Coffee bean oil 60 - - - - - 12 10 - Coffee bean extract - 60 - - - - 12 10 - Coffee tree leaf extract - - 60 - - - 12 10 - Green tea extract 60 - - 12 10 - Oolong tea extract - - - 60 - 12 10 Production Example 9 - - - - - 60- - 10 -

Experimental Example  1: Skin safety test

MTT assay experiments were performed on fibroblasts (HDF), melanocytes (B16F10), and keratinocytes (HaCaT) to verify skin safety for the samples of the Examples and Comparative Examples.

Samples of Examples and Comparative Examples were suspended in purified water at concentrations of 0, 1, 5, 10, 20, 50, and 100 mg / L, respectively, and cell viability was measured.

Cytotoxicity was performed by modifying the method of Mosmann to measure cell viability using MTT {3- (4,5-dimethylthiazol-2-yl) -2-5-diphenyltetrazolium bromide} reagent.

(HDF), melanocyte-forming cells (B16F10), and keratinocytes (HaCaT) were dispensed in 96-well plates at a concentration of 1 × 10 ⁴ cells / well and cultured at 37 ° C. and 5% CO ₂ for 48 hours Respectively.

After the culture medium was removed and the sample was cultured for 48 hours in a concentration-treated medium, the medium was removed and repeatedly washed with PBS (phosphate buffered saline).

MTT was dissolved in PBS at 5 mg / mL, 50 L was added, and the cells were cultured at 37 ° C and 5% CO ₂ for 48 hours. 100 L of DMSO (dimethyl sulfoxide) was added per well, stirred for 10 minutes, and absorbance was measured at 540 nm.

As a result of measurement, cytotoxicity was not confirmed at all concentrations of each sample. The results suggest that the sample is not only harmless to the human body but also has excellent human safety.

Experimental Example  2 : DPPH  Evaluation of free radical scavenging activity by measuring method

The extracts of Examples and Comparative Examples were suspended in purified water to evaluate free radical scavenging activity.

The DPPH assay is a method of measuring the absorbance at 540 nm of the extent to which the inhibitor decolorizes the stable radical DPPH (2,2-Diphenyl-1-picrylhydrazyl radical).

In the control group, ascorbic acid was used and the 50% scavenging concentration (SC ₅₀ ) of the radical DPPH was measured and compared with the control group (Table 3).

Particularly, Example 1 measured the free radical scavenging activity by concentration and compared with the control group (Fig. 1).

[Concentration in which 50% of radical DPPH is eliminated] division IC ₅₀ ( μg / mL) Example 1 73.5 Example 2 72.2 Example 3 72.0 Example 4 76.7 Example 5 77.7 Example 6 78.0 Example 7 71.4 Example 8 61.0 Example 9 59.4 Example 10 61.8 Example 11 62.3 Example 12 64.7 Example 13 63.7 Example 14 62.7 Example 15 63.6 Example 16 59.8 Comparative Example 1 105.0 Comparative Example 2 111.9 Comparative Example 3 110.2 Comparative Example 4 113.0 Comparative Example 5 116.8 Comparative Example 6 111.2 Comparative Example 7 102.1 Comparative Example 8 100.2 Comparative Example 9 96.3 Positive control group 84.8

The lower the IC ₅₀ value, the higher the inhibition rate of tyrosinase activity. The above tyrosinase plays an important role in the formation of the melanin pigment, and a high inhibitory activity of tyrosinase suggests that the whitening effect is excellent.

Referring to Table 3, the samples of Examples 1 to 16 effectively inhibited tyrosinase activity as compared with Comparative Examples 1 to 9.

In particular, fermented extracts (Examples 2 to 8) were further improved in thyrosinase activity inhibitory effect as compared with Example 1.

Experimental Example  3: ABTS  Evaluation of free radical scavenging activity by measuring method

Samples of Examples and Comparative Examples were suspended in purified water to evaluate free radical scavenging activity.

The ABTS method measures the degree of conversion of the antioxidant to colorless ABTS at 732 nm by dissolving the cyanic radical ABTS (2,2'-azinodi [3-ethylbenzthiazolne-6-sulfonic acid]).

The control group used vitamin C, and a 50% scavenging concentration (SC ₅₀ ) of the radical ABTS was measured and compared with the control group (Table 4).

[Concentration of radical abolition of 50% of ABTS] division SC ₅₀ ( μg / mL) Example 1 55.1 Example 2 45.0 Example 3 46.1 Example 4 50.9 Example 5 50.3 Example 6 49.4 Example 7 50.2 Example 8 52.0 Example 9 41.1 Example 10 41.1 Example 11 37.6 Example 12 35.7 Example 13 34.6 Example 14 34.0 Example 15 36.5 Example 16 31.7 Comparative Example 1 98.7 Comparative Example 2 95.0 Comparative Example 3 97.1 Comparative Example 4 99.0 Comparative Example 5 99.8 Comparative Example 6 96.9 Comparative Example 7 90.7 Comparative Example 8 86.7 Comparative Example 9 81.3 Control group (vitamin C) 19.6

The lower the value of SC _50, the higher the scavenging activity of free radicals. The higher the free radical scavenging activity, the better the antioxidative effect.

Referring to Table 4, the samples of Examples 1 to 16 effectively scavenged free radicals as compared with Comparative Examples 1 to 9.

In particular, the fermentation extracts (Examples 2 to 8) were further improved in the scavenging activity of the free radicals as compared to Example 1. [

Experimental Example  4: Wrinkle improvement evaluation

The effect of improving the wrinkles of the samples of Examples and Comparative Examples was evaluated through the inhibitory activity of elastease. The samples of Examples and Comparative Examples were suspended in purified water and the inhibitory activity of elastase was measured as follows

A 1.6 mM elastase substrate (N-succinyl- (Ala) 3-p-nitroanilide) (SANA) and 0.2 mM Tris-HCl buffer (pH 8.0) were prepared. 0.165 mL of the above Tris-HCl buffer, 0.005 mL of SANA, and 0.01 mL of ELASTIA were dispensed into 96 well plates.

Samples of the Examples and Comparative Examples were added to the positive control group, followed by reaction at 25 ° C for 15 minutes, and the absorbance was measured at 405 nm. Percent (%) of the elastase inhibitory activity was calculated as a percentage of the difference in absorbance with the addition of the sample to the absorbance without the sample.

As a positive control, oleanolic acid was used, and the concentration (IC ₅₀ ) at which 50% inhibition of elastase of the samples of Examples and Comparative Examples was measured was shown in Table 5.

Particularly, in Example 1, the inhibition rate of the elastase activity by concentration was measured and compared with the control group (FIG. 3).

[50% inhibitory concentration of elastase] division IC ₅₀ ( μg / mL) Example 1 391.8 Example 2 305.0 Example 3 304.9 Example 4 308.4 Example 5 309.3 Example 6 308.8 Example 7 308.9 Example 8 308.7 Example 9 293.1 Example 10 289.7 Example 11 293.8 Example 12 296.1 Example 13 295.2 Example 14 294.2 Example 15 292.2 Example 16 275.1 Comparative Example 1 522.1 Comparative Example 2 528.2 Comparative Example 3 535.3 Comparative Example 4 537.8 Comparative Example 5 549.7 Comparative Example 6 501.8 Comparative Example 7 493.8 Comparative Example 8 494.7 Comparative Example 9 480.1 Positive control group 64.8

The lower the value of IC _50, the higher the inhibition of elastase activity. The above-mentioned Elastase is an enzyme that degrades elastin, which is an important substrate for maintaining skin elasticity in the dermis. Elastase is also a nonspecific hydrolytic enzyme capable of degrading collagen, another important substrate protein.

Therefore, a high inhibition rate of elastase activity means that elastin and collagen, which are important for maintaining skin elasticity, are maintained for a long time, suggesting that the effect of improving skin wrinkles is excellent.

Referring to Table 5, the samples of Examples 1 to 16 effectively inhibited elastase activity as compared with Comparative Examples 1 to 9.

In particular, the fermented extracts (Examples 2 to 8) were further improved in inhibiting elastase activity than Example 1.

Experimental Example  5: Evaluation of skin elasticity improvement

The skin elasticity improving effect of the cosmetic composition containing the samples obtained in Examples and Comparative Examples was evaluated.

Each of the above samples was formulated into cream of the same composition, and samples of the Examples and Comparative Examples were different.

Healthy women aged 20 years or older (average age 37 years old) were divided into groups in the conditions of temperature of 24 to 26 캜 and humidity of 75%, and the creams of the examples and the comparative examples were respectively applied.

The formulated cream was applied for 12 weeks twice a day (morning and evening) around the eyes, and skin elasticity was measured using a skin elasticity analyzer (Cutometer MPA580, Conrage + Khazaka, Germany).

The test results are shown as R8 (R8 (12 weeks) -R8 (0 weeks)) value of cutometer MPA580, R8 value indicates the property of skin viscoelasticity, and the results are shown in Table 6 below

division Skin elasticity improvement effect Example 1 0.70 Example 2 0.75 Example 3 0.74 Example 4 0.75 Example 5 0.73 Example 6 0.74 Example 7 0.75 Example 8 0.83 Example 9 0.78 Example 10 0.81 Example 11 0.79 Example 12 0.85 Example 13 0.86 Example 14 0.86 Example 15 088 Example 16 0.91 Comparative Example 1 0.62 Comparative Example 2 0.44 Comparative Example 3 0.42 Comparative Example 4 0.64 Comparative Example 5 0.54 Comparative Example 6 0.65 Comparative Example 7 0.65 Comparative Example 8 0.67 Comparative Example 9 0.70 Positive control (vitamin C) 0.92 Negative control (no treatment) 0.31

Referring to Table 6, the samples of Examples 1 to 15 exhibited remarkably superior skin elasticity improving effect as compared with Comparative Examples 1 to 8.

In particular, the coffee silver-skin fermented extracts (Examples 2 to 8) significantly improved the skin elasticity effect compared to the coffee silver skin extract (Example 1), and the results showed that the coffee silver- .

In addition, the samples of the examples including the coffee silver-silver fermented extract and the combined extract showed the same level of improvement in skin elasticity as the positive control vitamin C.

Experimental Example  6: Evaluation of skin soothing effect (skin temperature reduction rate)

In order to evaluate the anti-aging effect of the cosmetic composition containing the samples obtained in the above Examples and Comparative Examples, the following experiment was conducted.

Each of the above samples was formulated into cream of the same composition, and samples of the Examples and Comparative Examples were different.

20 to 30 healthy subjects were exposed to direct sunlight for 20 minutes before the test.

Test specimens and control specimens were applied to the selected test site (2 cm × 2 cm) of the forearm using a micropipette in an amount of 50 μL, and the temperature was measured before application of the sample, immediately after application, 5 minutes after application and 20 minutes after application Respectively.

Water was used as a negative control for comparison of anti-aging efficacy. The temperature decrease rate of each sample after the measurement was derived according to the following equation.

[Mathematical Expression]

Thermal Reduction Rate (%) = (1 - Temperature of sample application site / Temperature of non-use site) x 100

[Heat reduction rate (%)] division Immediately after application 5 minutes later After 20 minutes Control (water) 5.8 0.6 0 Example 1 13.1 4.1 1.2 Example 2 15.4 7.2 2.3 Example 3 14.9 6.9 1.9 Example 4 16.5 6.5 1.5 Example 5 15.6 6.3 1.7 Example 6 16.2 7.1 2.1 Example 7 15.8 6.6 1.4 Example 8 15.6 6.9 1.5 Example 9 17.2 7.3 2.5 Example 10 17.5 7.1 2.1 Example 11 17.2 7.3 2.3 Example 12 16.9 6.8 1.9 Example 13 17.1 6.9 1.8 Example 14 17.9 7.1 2.1 Example 15 17.2 7.2 2.0 Example 16 18.5 8.2 2.8 Comparative Example 1 9.5 2.7 0.1 Comparative Example 2 9.2 2.5 0.2 Comparative Example 3 8.6 1.9 0.2 Comparative Example 4 9.4 2.2 0.1 Comparative Example 5 10.3 1.7 0.2 Comparative Example 6 7.4 2.5 0.1 Comparative Example 7 9.6 3.6 0.2 Comparative Example 8 10.5 3.4 0.1 Comparative Example 9 11.3 2.3 0.3

Referring to Table 7, the temperature reduction rates of the samples 1 to 16 were significantly superior to those of Comparative Examples 1 to 9, and the skin soothing effect was evaluated to be high.

In particular, the fermented extracts (Examples 2 to 8) were significantly improved in skin soothing effect as compared with Example 1.

Experimental Example  7: Evaluation of collagen synthesis amount

The effect of collagen synthesis on the samples of 1.0% concentration obtained in the above Examples and Comparative Examples was measured.

Human normal fibroblast cells were seeded at 1 × 10 ⁵ cells / well in a 24-well plate, cultured for 24 hours in 10% FBS / DMEM medium, and irradiated with infrared light.

Each extract was treated with serum free medium and cultured in a CO ₂ incubator for 24 hours. The amount of collagen synthesized was measured by using a Procollagen Type C-peptide EIA kit (Takara, Japan), and the amount of collagen synthesized was measured.

As a negative control for comparison of anti-aging effects, infrared irradiation was performed without a sample. As a positive control, 0.3 mM ascorbic acid (vitamin C) was used.

division Collagen synthesis amount (ng / mL) Example 1 133.2 Example 2 137.5 Example 3 136.1 Example 4 135.7 Example 5 138.4 Example 6 137.2 Example 7 136.6 Example 8 138.2 Example 9 142.8 Example 10 144.6 Example 11 141.9 Example 12 140.5 Example 13 143.3 Example 14 141.6 Example 15 142.5 Example 16 145.2 Comparative Example 1 114.8 Comparative Example 2 116.5 Comparative Example 3 119.4 Comparative Example 4 117.6 Comparative Example 5 119.2 Comparative Example 6 118.5 Comparative Example 7 120.2 Comparative Example 8 124.5 Comparative Example 9 127.3 Negative control group 105.3 Positive control group 141.4

Referring to Table 8, the amounts of collagen synthesis according to the applications of Examples 1 to 16 were significantly higher than those of Comparative Examples 1 to 9, suggesting that the above results are excellent in suppressing and improving skin damage caused by heat aging .

In particular, the fermented extracts (Examples 2 to 8) promoted the collagen synthesis amount more effectively than Example 1.

It will be understood by those skilled in the art that the foregoing description of the present invention is for illustrative purposes only and that those of ordinary skill in the art can readily understand that various changes and modifications may be made without departing from the spirit or essential characteristics of the present invention. will be. It is therefore to be understood that the above-described embodiments are illustrative in all aspects and not restrictive. For example, each component described as a single entity may be distributed and implemented, and components described as being distributed may also be implemented in a combined form.

The scope of the present invention is defined by the appended claims, and all changes or modifications derived from the meaning and scope of the claims and their equivalents should be construed as being included within the scope of the present invention.

Claims (9)

Coffee silver skin and coffee bean extract as an active ingredient. The method according to claim 1,
Wherein the extract is a fermented extract prepared by inoculating a fermentation strain. 3. The method of claim 2,
The fermentation strains were obtained from Hericium < RTI ID = 0.0 > erinaceum , Pleurotusus ferulae , Sparassis crispa , Ganoderma lucidum , Flammulina velutipes ), mushroom ( Phellinus linteus and Grifola frondosa . < RTI ID = 0.0 > 1. < / RTI > The method according to claim 1,
Wherein the cosmetic composition further comprises at least one selected from the group consisting of coffee bean oil, coffee bean extract, coffee bean powder, coffee tree leaf extract, coffee tree fruit extract and coffee tree bud extract. The method according to claim 1,
Wherein the composition further comprises at least one selected from the group consisting of green tea extract, oolong tea extract, Boi tea extract, black tea extract, and black tea extract. The method according to claim 1,
A cosmetic composition further comprising a camphor flower extract, a camphor flower extract, and a lotus flower extract. 7. The method according to any one of claims 1 to 6,
A cosmetic composition for preventing heat aging. 7. The method according to any one of claims 1 to 6,
A cosmetic composition for skin elasticity and wrinkle improvement. 6. The method according to any one of claims 1 to 5,
Nourishing lotion, nutrition lotion, water cream, nutritional cream, massage cream, nutrition lotion, essence, ampoule, gel, eye cream, oil, foundation, wax, cleansing cream, cleansing foam, cleansing water, shampoo, rinse, pack, hair cream , Hair wax, hair gel, spray, and powder.