KR101308669B1 - Cosmetic composition with the nebaneba complex of hibiscus esculentus, laminaria japonica, dioscorea opposita, corchorus olitorius and nelumbo nucifera, polyglutamic acid - Google Patents

Abstract

PURPOSE: A cosmetic composition containing neba-neba complex including Hibiscus esculentus, Laminaria japonica, Dioscorea opposita, Corchorus olitorius, Nelumbo nucifera, and polyglutamic acid is provided to remarkably treat deterioration of skin and to improve anti-wrinkling and anti-aging effects with excellent antioxidation. CONSTITUTION: A cosmetic composition contains polyglutamic acid and an extract containing Hibiscus esculentus, Laminaria japonica, Dioscorea opposita, Corchorus olitorius, and Nelumbo nucifera as active ingredients. The extract is prepared by separately extracting Hibiscus esculentus, Laminaria japonica, Dioscorea opposita, Corchorus olitorius, and Nelumbo nucifera or mixing and extracting the ingredients using a solvent selected among water, anhydrous or hydrous C1-C4 alcohol, acetone, ethyl acetate, butylacetate, and 1,3-butylene glycol.

Description

Cosmetic composition comprising Neva Neva complex composed of okra, kelp, yam, moro haya, lotus root, polyglutamic acid acid}

The present invention relates to a cosmetic composition comprising a so-called 'Neva Neva complex' composed of polyglutamic acid is added to the extract of okra, kelp, yam, morohayiya and lotus root as an active ingredient.

Oxygen, along with water, is an indispensable substance in our lives. However, once in our body, oxygen turns into highly active free radicals that oxidize blood vessel cells for a number of reasons. Today, about 90% of disease causes are identified by these free radicals.

The human body is composed of about 60 trillion cells. The cell membrane is formed of the cortex, and free radicals are easily connected. When oxidation occurs, unsaturated fatty acids turn into peroxide. This lipid peroxide damages genes DNA, RNA, proteins, cell membranes, and cellular structures, leading to cancer or the development of various adult diseases, ultimately causing aging. Antioxidants are substances that act to remove or neutralize these free radicals and prevent the cells from oxidizing.

When lipids, which are components of skin cells, are peroxidated due to free radicals, the structure of the cell membrane is altered, resulting in cell permeability, fluidity, inactivation of membrane enzymes and transport proteins, cleavage of protein chains, DNA and RNA damage, etc. This is known to cause aging of skin cells. For this reason, efforts to remove free radicals have continued, and to date many antioxidants have been developed.

The substances known to have antioxidant activity so far include ascorbic acid, beta-carotene, dibutylhydroxytoluene (BHT), DL-alpha-tocopherol, and the like. . However, these substances are synthetic materials, which may cause problems of skin safety in long-term use or in large amounts on the skin, or may cause stability problems when added to cosmetics. Many researchers in the cosmetics industry is trying to solve these problems.

On the other hand, cosmetics is a sector that combines science with the odor, feeling, container, etc., because it is a consumer's favorite product. The dual usability can be subdivided into spreading, spreading, moist, sticky, cushioning, etc., due to the nature of the cosmetics are mixed with a variety of raw materials is a problem that is inevitable due to the mixing of raw materials is inevitable. Accordingly, many researchers in the cosmetics industry are trying to solve these problems of skin feel.

Korean Patent Publication No. 10-1996-0020979 (published date: July 18, 1996) relates to a cosmetic product that has a good makeup, gives a moist feeling, and improves the feeling of use due to excellent adhesion and durability. As cosmetics in which N-lauroyl-L-lysine is heat-treated or mixed-dispersed together, cosmetics having high water repellency by the action of two components are described.

The present inventors conducted a lot of research to invent a substance effective for improving skin function and feel, and when the composition is prepared by adding polyglutamic acid to extracts of okra, kelp, yam, moroheiya and lotus root, The present invention has been found to be excellent in improving various skin functions and usability.

Accordingly, the present invention is to develop and provide a cosmetic composition with improved skin functionality and usability using Neva Neva complex prepared from okra, kelp, yam, yam, moroheiya, lotus root extract and polyglutamic acid.

The present invention provides a cosmetic composition comprising a mixture consisting of polyglutamic acid to the extract of okra, kelp, yam, morohayiya and lotus root as an active ingredient.

On the other hand, in the cosmetic composition of the present invention, the extracts of okra, kelp, yam, moroheiya and lotus root are mixed after extracting okra, kelp, yam, moroheiya and lotus root, respectively, or okra, kelp, After mixing the yam, moroheiya and lotus root, it may be prepared by extraction.

On the other hand, in the cosmetic composition of the present invention, the cosmetic composition may be for preventing skin aging.

On the other hand, in the cosmetic composition of the present invention, the cosmetic composition may be for improving skin feel.

On the other hand, in the cosmetic composition of the present invention, the cosmetic composition may be for skin elasticity enhancement.

On the other hand, in the cosmetic composition of the present invention, the cosmetic composition may be for skin moisturizing.

On the other hand, in the cosmetic composition of the present invention, the cosmetic composition may be for skin wrinkle improvement.

On the other hand, in the cosmetic composition of the present invention, the cosmetic composition may be for skin barrier strengthening.

On the other hand, in the cosmetic composition of the present invention, the extract is any one selected from the group consisting of water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate and 1,3-butylene glycol It may be extracted using an extraction solvent.

On the other hand, in the cosmetic composition of the present invention, the mixture may be added to 0.0001 to 100.0% by weight based on the total weight of the cosmetic composition.

On the other hand, in the cosmetic composition of the present invention, the cosmetic composition is a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation And it may have one of the formulations selected from the group consisting of a spray.

The nevaneva complex (ocra, kelp, yam, moroheiya, extract of lotus root and polyglutamic acid) cosmetic composition of the present invention exhibits an effect that can significantly improve the problem of deterioration of the skin feeling that is problematic with the addition of various raw materials. .

In addition, the cosmetic composition of the present invention exhibits an excellent antioxidant effect to improve skin wrinkles and prevent skin aging.

In addition, the cosmetic composition of the present invention exhibits excellent effects on skin elasticity enhancing effect, skin moisturizing and skin barrier strengthening.

The present inventors prepared extracts by various extraction methods using a plurality of natural products as a raw material, and conducted a study for selecting substances having excellent effects such as antioxidant effect, moisturizing and improved feeling of use. As a result, it was found that the composition prepared by adding polyglutamic acid to extracts of okra, kelp, yam, moroheiya, and lotus root (hereinafter referred to as 'Nevanevac complex') is most suitable for the above-described object of the present invention. Was done.

Cosmetic composition of the present invention comprises Neva Neva complex as an active ingredient.

Okra ( Hibiscus Esculentus ) Pods can be eaten as vegetables or used as thickening agents to thicken other foods. It has an elegant shape and is also called 'lady's fingers' in English. The world's largest producer of okra is the kamikaze region of Japan, which is said to be healthy because it enjoys eating okra, and it is loved enough to be called the taste of Japan. During the Second World War, the supply of coffee beans became short and Okra seeds were used as coffee substitutes. As ingredient Vitamin A, B, C, potassium, mucin and abundantly contained, and various vitamins are known to suppress fatigue and anti-inflammatory and inhibit acne.

Kelp ( Laminaria) Japonica ) It is called sea vegetable, and it is farmed in Jeonnam sea area, and you can get moisturizing fucoidan. It is known as an algae that has been handed down to the civilian since ancient times, and it is known not only for the prevention of arteriosclerosis and myocardial infarction but also for its anticancer effect and for promoting hair growth. Alginic acid, laminarin, fucoidan, vitamin C, amino acids, iodine and many more.

Yam ( Dioscorea opposita ) is a perennial vine grass of Dioscoreaceae genus vine root and its root is called as a tonic. In the mountains, I'm called eel rich in nutrients and active ingredients. It contains a lot of saponins, arginine, mucins, amino acids and diosgenin.

Morchoeirai ( Corchorus Olitorius ) is called Moroheiya in Egypt, but originally Molokia , the Arabic word ' Mor ' means 'Kor' and 'Kia' means 'Mor''Vegetables', that is, it is known to mean vegetables that only the royal family can eat without being open to the public. Since it grows well in the hot, dry, tropical deserts, it has long been considered a valuable green vegetable in the tropics. In ancient Egypt, it is a familiar vegetable in Egyptian life, enough to chop leaves into hammocks. It contains a lot of vitamins A, B, C, E, mucin and the like.

Lotus root ( Nelumbo Nucifera ) is the root of lotus, and lotus is the edible root of lotus root. Lotus root is a lotus root that grows in a shallow pond and is used after digging roots from autumn to early spring. Since ancient times it has been loved a lot for food or medicinal use. It contains a lot of tannins, vitamin C and mucin.

Polyglutamic acid (Polyglutamic acid) is an edible amino acid polymer material and is a sticky mucus substance found in fermented foods such as Korea's own Cheonggukjang and natto in Japan. It is a highly water soluble and biodegradable anionic polymer. It is known to enhance natural moisturizing factor (NMF) to enhance skin moisturizing effect, inhibit the activity of enzymes that break down hyaluronic acid, relieve inflammation of the skin and improve itching.

To date, the extracts of okra, kelp, yams, moro heyiya, lotus root, and polyglutamic acid are used for many different purposes as described above. However, as in the present invention, a mixture form, i.e., nevaneva complex (Okra, kelp, yam, morohay, extract of lotus root and polyglutamic acid) is significantly higher in antioxidant and skin aging than when using each of the above extracts and compounds alone. It is not known whether the skin elasticity improvement, moisturizing feeling, skin barrier strengthening and feeling improvement effect are effective.

Cosmetic composition comprising Neva Neva complex composed of okra, kelp, yam, moro hayiya, extract of lotus root and polyglutamic acid of the present invention has an excellent antioxidant effect to improve skin wrinkles, showing excellent skin anti-aging effect, skin elasticity It is effective in enhancing skin moisturizing, enhancing skin barrier and improving skin feel.

In Nevane Complex, which is an active ingredient of the cosmetic composition of the present invention, the extract can be prepared according to a conventional method known in the art, that is, using a conventional solvent, under the conditions of conventional temperature and pressure. That is, the extracts of okra, kelp, yam, yam, moroheiya and lotus root of the active ingredients of the composition of the present invention, after mixing them, water, anhydrous or hydrous lower alcohols having 1 to 4 carbon atoms, acetone, ethyl Extracted using a solvent selected from the group consisting of acetate, butyl acetate and 1,3-butylene glycol, or each of okra, kelp, yam, moroheiya and lotus root using the above solvent, and then prepared by mixing them Can be.

Among the solvents, alcohol is preferable, and ethanol is more preferable. At this time, the ethanol is preferably 70% ethanol. Suitable amounts of the extraction solvent are 1-15 times the dry weight of okra, kelp, yams, moroheiya, lotus root, more preferably 5-10 times, most preferably 7 times.

The Nevane complexes of the present invention (okra, kelp, yam, morohay, extract of lotus root, polyglutamic acid) can be prepared by a process comprising the following steps as an example.

(a) consisting of okra, kelp, yam, morohay, 1 to 10 times water in lotus root, anhydrous or hydrous lower alcohol of 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate and 1,3-butylene glycol Adding any one solvent selected from the group;

(b) extracting the solvent-added okra, kelp, yam, moroheiya, and lotus root of step (a) at 4-100 ° C. for 1 hour to 5 days;

(c) filtering the extract of step (b) and then aging for 7-10 days at 3-5 ° C., followed by filtration;

(d) drying the filtrate of step (c) with a rotary evaporator,

(e) adding polyglutamic acid.

At this time, when 1,3-butylene glycol is used as the extraction solvent, it is difficult to dry using a rotary decompression evaporator, so that the drying loss after the extraction step (b) is adjusted to 1% (w / v). It is also necessary.

On the other hand, the Neva Neva complex of the present invention includes not only the extract by the above-described extraction method, but also extracts that have undergone a conventional purification process. Obtained by various additional purification methods, such as, for example, separation using an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity). Fractions are also construed as being included in the Nevaeva complex of the present invention.

In addition, the Nevaeva complex of the present invention may be prepared in a powder state by an additional process such as vacuum distillation and freeze drying or spray drying.

According to a preferred embodiment of the present invention, the content of the Nevaeva complex added to the cosmetic composition is 0.0001 to 100.0% by weight, preferably 0.001 to 60.0% by weight, most preferably 0.1 to 30.0% by weight of the total cosmetic composition Weight percent. When the content of the Neva Neva complex is less than 0.0001% by weight there is a problem in showing the antioxidant effect and the feeling of improving the feeling.

On the other hand, the cosmetic composition of the present invention as an active ingredient may include components commonly used in cosmetic compositions in addition to the Nevaeva complex, for example, conventional auxiliary agents such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavorings. And a carrier.

On the other hand, the cosmetic composition of the present invention can be prepared in any formulation commonly prepared in the art, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactants- Formulated with containing cleansing, oils, powder foundations, emulsion foundations, wax foundations, sprays and the like. However, the present invention is not limited thereto. More specifically, it may be prepared in the form of a flexible lotion, astringent lotion, nutrition lotion, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder.

In case the formulation of the cosmetic composition of the present invention is a paste, cream or gel, the carrier component is animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide. This can be used.

In addition, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component when the formulation of the cosmetic composition of the present invention is a powder or a spray, and in particular, in the case of a spray, additionally chlorofluoro Propellants such as rohydrocarbon, propane / butane or dimethyl ether.

In addition, when the formulation of the cosmetic composition of the present invention is a solution or emulsion, a solvent, a solubilizing agent or an emulsifying agent is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate. Fatty acid esters of propylene glycol, 1,3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

In addition, when the formulation of the cosmetic composition of the present invention is a suspension, a liquid diluent such as water, ethanol or propylene glycol, ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbitan ester are used as carrier components. Suspending agents, microcrystalline cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

In addition, when the formulation of the cosmetic composition of the present invention is a surfactant-containing cleansing agent, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, an isethionate, an imidazolinium derivative, a methyltaurate, a sarco Cinates, fatty acid amide ether sulfates, alkylamidobetaines, fatty alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters, and the like.

On the other hand, the Nevane complexes of the present invention (okra, kelp, yam, morohay, extract of lotus root, polyglutamic acid) exhibit an antioxidant effect (see Experimental Example 1). In addition, in the case of the cosmetic composition prepared by containing the Neva Neva complex having the effect as an active ingredient, the effect of improving the feeling (Reference: Experimental Example 2), excellent skin wrinkle improvement effect (Reference: Experimental Example 3), skin elasticity effect (Reference: Experimental Example 4) was found to be excellent.

Hereinafter, the content of the present invention will be described in more detail through the following examples. However, the following examples are intended to illustrate the present invention in more detail, and the scope of the present invention is not limited only to the following examples.

[ Example  1: Neva Neva Complex ( okra , Kelp, yam , Moroheiya , Extracts of lotus root and Polyglutamic acid Manufacture)

After washing with purified water and crushing 10.0 g each of dried okra, kelp, yam, moroheiya, and lotus root, it was poured into 1.2 l of water and extracted at 65 ° C. for 5 hours, filtered with 300 mesh filter cloth, and then filtered at 4 ° C. for 5 days. After standing to age, it was filtered by Whatman No. 5 filter paper. The filtrate was dried on a rotary immersion evaporator at 65 ° C. to obtain a dry weight of 12.83 g, after which about 2 g of polyglutamic acid was added to obtain Nevane complex (polyglutamic acid, okra, kelp, yam, moroheiya, lotus root extract). Got it.

[ Example  2 to 19: Neveva complex  Produce]

Except that the extraction solvent of Table 1 was used, the extraction was carried out in the same manner as in Example 1 to obtain the Nevane complexes of Examples 2 to 19.

extract Extraction solvent Dry weight of the final extract
(Unit: g) Example 2 10% ethanol 12.8 Example 3 20% ethanol 13.9 Example 4 30% ethanol 13.2 Example 5 40% ethanol 14.3 Example 6 50% ethanol 15.1 Example 7 60% ethanol 15.3 Example 8 70% ethanol 14.2 Example 9 80% ethanol 13.3 Example 10 90% ethanol 12.5 Example 11 100% ethanol 13.4 Example 12 Methanol 13.3 Example 13 n-propanol 11.5 Example 14 Isopropanol 10.6 Example 15 2-butanol 4.2 Example 16 Acetone 3.5 Example 17 chloroform 2.5 Example 18 Ethyl acetate 3.3 Example 19 Butyl acetate 2.6

[ Comparative example  1-10]

10.0 g of okra, kelp, yam, moroheiya, and lotus root washed with purified water were crushed, and 500 ml of water was separately added and extracted at 65 ° C. for 5 hours, followed by filtration with 300 mesh filter cloth and at 4 ° C. After aging for 5 days, the resultant was filtered using Whatman 5 filter paper. The filtrate was dried with a rotary evaporator at 65 ° C. to obtain dry weights of okra, kelp, yam, moroheiya and lotus root extracts as shown in Table 2 below. Meanwhile, in Comparative Example 6, polyglutamic acid 2.0 g was prepared, and Comparative Examples 7 to 10 prepared 2.0 g, respectively, according to the components and ratios shown in Table 2 below.

extract Dry weight of the final extract
(Unit: g) Comparative Example 1 (Okra) 1.2 Comparative Example 2 (Kashima) 1.3 Comparative Example 3 (Yama) 0.8 Comparative Example 4 (Moroheiya) 1.5 Comparative Example 5 (Lotus Root) 2.3 Comparative Example 6 (Polyglutamic Acid) 2.0 Comparative Example 7 2.0 (Okra: Kashima = 1: 1) Comparative Example 8 2.0 (Okra: Tashima: Yama = 1: 1: 1) Comparative Example 9 2.0 (Okra: Tashima: Yuma: Moroheiya = 1: 1: 1: 1) Comparative Example 10 2.0 (Okra: Tashima: Yuma: Moroheiya: Lotus Lotus = 1: 1: 1: 1: 1)

[ Example  20: Supercritical  Using fluid extraction Neveva complex  Produce]

Okra, kelp, yams, morohay, and lotus root were washed with purified water, dried and crushed. Subsequently, these samples were mixed to prepare a sample, and 100 g of the sample was placed in a supercritical fluid extractor (Jasco, Japan) and pure carbon dioxide and a cosolvent were prepared by using a liquid chromatography pump (Supercritical fluid chromatograph, Jasco, Japan). 10 ml of the extract for up to 40 minutes every 4 minutes, using a mixture of alcoholic spirits and non-polar solvents squalene in a 1: 1 ratio at 80 ° C and 300 atmospheres. Received in glass test tubes. Then, the same ratio of polyglutamic acid was added to prepare Nevane complexes (okra, kelp, yam, morohay, extract of lotus root and polyglutamic acid).

[ Experimental Example  One: Neveva complex  Antioxidative effect]

DPPH antioxidant experiments were performed to investigate the antioxidant effects of Neva Neva complex. A DPPH (1,1-diphenyl-2-picrylhydrazyl radical) solution (60 M) was prepared. Then, the concentration of Neva Neva complex (Examples 1-20) was adjusted to 1%, and the DPPH solution was mixed at a 1: 1 ratio, and then vortexed (vortex mixed). After reacting at room temperature for 30 minutes, absorbance was measured at 520 nm to obtain free radical scavenging activity (SC) according to the following formula (2). Controls were measured using ethanol instead of samples.

[Equation 1]

% Free radical scavenging activity = (control absorbance-example absorbance) / (control absorbance) × 110

sample Free radical scavenging activity (%) Control group - Comparative Example 1 55.23 Comparative Example 2 44.32 Comparative Example 3 55.77 Comparative Example 4 65.32 Comparative Example 5 42.56 Comparative Example 6 56.12 Comparative Example 7 55.23 Comparative Example 8 61.23 Comparative Example 9 62.35 Comparative Example 10 61.21 Example 1 92.33 Example 2 95.77 Example 3 87.98 Example 4 87.23 Example 5 88.21 Example 6 89.33 Example 7 89.33 Example 8 87.29 Example 9 89.45 Example 10 93.55 Example 11 94.33 Example 12 95.11 Example 13 86.77 Example 14 92.55 Example 15 87.33 Example 16 93.77 Example 17 90.55 Example 18 93.44 Example 19 91.23 Example 20 91.57

As can be seen from the antioxidant effect measurement results of Table 3, the extract of the present invention exhibits a synergistic effect than the extract (Comparative Examples 7 to 10) combined with only a portion of each extract (Comparative Examples 1 to 6), and very excellent oxidation. Inhibition rate and free radical scavenging activity were shown. Thus, it was found that the example composition of the present invention exhibited an excellent antioxidant effect.

[ Preparation Example 1 : Nevaeva complex  Containing Cosmetic  Produce]

Preparation of the cosmetic composition containing 10.0% by weight of the Neva Neva complex of Example 8 to the composition of the following Table 4, which is referred to as Formulation Example 1, to prepare a cosmetic composition does not contain the Neva Neva complex Comparative Comparative Examples 1-4 It was.

ingredient Prescription Example 1
Content (% by weight) Comparative Prescription 1
Content (% by weight) Comparative Prescription 2
Content (% by weight Comparative Prescription 2
Content (% by weight Comparative Prescription 3
Content (% by weight Neva Neva complex (Example 8) 10.0 - - - - 1,3-BG - - 10 - - Comparative Example 8 - - - 10 - Comparative Example 10 - - - - 10 glycerin 5.1 5.1 5.1 5.1 5.1 Propylene glycol 4.2 4.2 4.2 4.2 4.2 Tocopheryl acetate 3.0 3.0 3.0 3.0 3.0 Liquid paraffin 4.6 4.6 4.6 4.6 4.6 Triethanolamine 1.0 1.0 1.0 1.0 1.0 Squalane 3.1 3.1 3.1 3.1 3.1 Macadamia nut oil 2.5 2.5 2.5 2.5 2.5 Polysorbate 60 1.6 1.6 1.6 1.6 1.6 Sorbitan sesquiterate 1.6 1.6 1.6 1.6 1.6 Propylparaben 0.6 0.6 0.6 0.6 0.6 Carboxyl vinyl polymer 1.5 1.5 1.5 1.5 1.5 incense a very small amount a very small amount a very small amount a very small amount a very small amount antiseptic a very small amount a very small amount a very small amount a very small amount a very small amount Purified water Balance Balance Balance Balance Balance system 100 100 100 100 100

[ Experimental Example  2: Nevaeva complex  Containing Cosmetic Feeling  Evaluation of improvement effect]

The effect of improving the feeling of use of the cosmetic of the present invention was evaluated through an actual use test. Evaluation of feeling improvement effect was carried out as follows. Twenty 30-40 year old women were randomly divided into two groups, and the creams of Preparative Examples 1-4 compared to Formula 1 prepared in Preparation Example 1 were washed twice each morning and evening, and then the appropriate amount of cream was continuously corrected around the eyes. It was. Each subject's feelings were evaluated through a user questionnaire. Each evaluation item was evaluated with a perfect score of 10, and the results shown in Table 5 were obtained.

sample Brittle Stickiness Cushion feeling Softness Adhesiveness Prescription Example 1 9.3 2.1 8.8 9.9 9.4 Comparative Prescription 1 4.5 6.3 5.6 6.7 7.4 Comparative Prescription 2 5.5 5.3 4.5 5.5 6.1 Comparative Prescription 3 6.5 4.3 4.6 7.7 6.4 Comparative Prescription 4 5.5 4.3 6.6 7.7 7.4

According to the results of Table 5, the cream of the present invention containing Neva Neva complex (prescription example 1) is 1,3-butylene glycol commonly used as a moisturizer and a feeling improving agent in cosmetics (comparative example 2) Compared with), the applicability, cushioning, softness, and adhesion are excellent, and it is confirmed that the cosmetic use is excellent, and the stickiness is very small. In addition, it was confirmed that the overall feeling is better than the comparative prescription examples 2 to 3 using only a part of the Nevaeva complex.

Therefore, it was confirmed that the Neva Neva complex extract of the present invention has a very excellent effect as a feeling-improving agent for cosmetics.

[ Experimental Example  3: Nevaeva complex  Containing Cosmetic  Wrinkle improvement effect evaluation]

The wrinkle-improving effect of the cosmetic of the present invention was evaluated through practical use tests. Wrinkle improvement effect was evaluated for Formulation Example 1 and Comparative Formulation Example 1 prepared in Preparation Example 1.

Wrinkle improvement effect evaluation was performed as follows. Twenty 30-40 year old women were randomly divided into two groups and the creams of Formulation Example 1 and Comparative Example 1 were washed twice every morning, and then the appropriate amount of cream was applied continuously for 2 months around the eyes. Wrinkle improvement effect of each subject was evaluated through visual observation. The experimental results were as shown in Table 6 below.

sample Excellent wrinkle improvement Wrinkle improvement No wrinkle improvement Effective rate (%) Prescription Example 1 8 8 4 70.0 Comparative Prescription 1 One 3 16 45.0

According to the results of Table 6, the cream of the present invention containing the Neva Neva complex (prescription example 1) showed a higher wrinkle improvement effect than the cream (Comparative Example 1) does not contain the Neva Neva complex.

[ Experimental Example  4: evaluation of skin elasticity enhancing effect]

The skin elasticity effect of the cosmetics of the present invention was evaluated through an actual use test. It evaluated using the prescription example 1 and comparative prescription example 1 which were manufactured by the manufacture example 1. Thirty 30 to 40 year old women were randomly divided into two groups and the creams of Formulation Example 1 and Comparative Example 2 were washed twice daily each morning and evening, and then the appropriate amount of cream was applied continuously for two months centering on both cheeks. Cutometer (C + K, Germany) was used to evaluate the skin elasticity of each subject. The experimental results are shown in Table 7 below.

Excellent elasticity promoting effect Little elasticity No elasticity promoting effect Effective rate (%) Cream of prescription example 1 14 11 6 83.3 Comparative Preparation 2 Cream 3 5 22 26.7

As a result of the experiment, the cream of Formula 1 containing the Neva Neva complex of the present invention showed a higher skin elasticity enhancing effect than the cream of Comparative Example 2 containing no Neva N complex.

[ Experimental Example  5: skin barrier function recovery effect]

In order to examine the effect of restoring the skin barrier function of the cosmetic containing Neva Neva complex of the present invention was carried out as follows. The experiment was conducted by using the taping stripping method on the upper arm of the human body. After applying the creams of Formulation Example 1 and Comparative Formulation Examples 1 to 4, respectively, prepared in Preparation Example 1 to an area of 5 cm 2, epidermal moisture loss was measured after 1 hour, 2 hours, 4 hours, and 8 hours. The extent to which barrier function is restored is assessed by evaluating the extent to which it is reduced. At this time, a known lipid mixture (ceramide: 2: 1: 1 mixture of cholesterol: fatty acid) was used as a positive control. The results are shown in Table 8 below comparing the change over time based on the amount of epidermal moisture loss in the initial damaged barrier state (100%).

sample 0 hours 1 hours 2 hours 4 hours 8 hours Prescription Example 1 85 53 25 20 20 Comparative Prescription 1 85 74 56 45 37 Comparative Prescription 2 85 69 55 41 34 Comparative Prescription 3 85 72 50 39 28 Positive control group 85 65 47 35 25

As a result of the experiment, as shown in the results of Table 8, in the case of Formulation Example 1, a cosmetic containing the Neva Neva complex of the present invention, it was confirmed that the skin barrier function recovery effect is very excellent compared to Comparative Examples 1-3. .

[ Experimental Example  6: skin Moisturizing  Improvement effect]

In order to find out the effect of improving the skin moisturizing power of the cosmetic containing Neva Neva complex of the present invention was carried out as follows. For 25 people in their 20s to 40s without skin diseases, divide into two sets of 20 people per set, and the nutrition cream of Formula 1 and Comparative Prescription 1 to 3 prepared in Preparation Example 1 for each group Twice daily for 1 month on face and forearm. The skin conductivity was measured using a corneometer (CM820 courage Khazaka electronic GmbH, Germany) under the constant temperature and humidity conditions (24 ° C, 40% humidity) before the start of application, and the skin conductivity was measured at 1 week, 2 weeks, The rate of increase in skin conductivity (%) was measured and the rate of increase was evaluated. The results are shown in Table 9 below.

sample After 1 week after 2 weeks After 4 weeks Prescription Example 2 57 63 65 Comparative Prescription 1 27 32 39 Comparative Prescription 2 24 35 44 Comparative Prescription 3 28 40 46

As shown in the results of Table 9, in the case of Formulation Example 1, which is a cosmetic containing the Neva Neva complex of the present invention, the skin conductivity increase rate was very excellent compared to Comparative Examples 1-3. In general, the skin conductivity is proportional to the moisture content of the skin, and the above results confirm that the cosmetic containing Nevane complex maintains the skin moisture content higher than that of the non-cosmetic cosmetic.

Claims (11)

Cosmetic composition comprising a mixture consisting of polyglutamic acid to the extract of okra, kelp, yam, moroheiya and lotus root as an active ingredient.
The method of claim 1,
The extract of okra, kelp, yam, moroheiya and lotus root,
Cosmetic composition, characterized in that the oak, kelp, yam, moroheiya, lotus root, respectively, after being extracted or mixed, or mixed with okra, kelp, yam, moroheiya and lotus root, and then extracted.
The method of claim 1,
In the cosmetic composition,
Cosmetic composition for preventing skin aging.
The method of claim 1,
In the cosmetic composition,
Cosmetic composition, characterized in that for improving skin feel.
The method of claim 1,
In the cosmetic composition,
Cosmetic composition, characterized in that for enhancing skin elasticity.
The method of claim 1,
In the cosmetic composition,
Wherein the composition is for moisturizing the skin.
The method of claim 1,
In the cosmetic composition,
Cosmetic composition, characterized in that for improving skin wrinkles.
The method of claim 1,
In the cosmetic composition,
Cosmetic composition, characterized in that for strengthening the skin barrier.
3. The method according to claim 1 or 2,
The above-
Cosmetic composition, characterized in that extracted using any one extraction solvent selected from the group consisting of water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate and 1,3-butylene glycol .
The method of claim 1,
The mixture may contain,
Cosmetic composition, characterized in that 0.0001 to 100.0% by weight based on the total weight of the cosmetic composition.
The method of claim 1,
In the cosmetic composition,
Having any one formulation selected from the group consisting of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations and sprays Cosmetic composition, characterized in that.