KR102304977B1 - Functional cosmetic composition containing a complex of fermented extract of natural products and a method for producing the same - Google Patents

Abstract

The present invention relates to a functional cosmetic composition prepared by fermenting a complex extract of citron, bellflower, and arrowroot, which are natural products, and a method for preparing the same.

Description

Functional cosmetic composition containing natural product complex fermented extract and manufacturing method thereof

The present invention relates to a functional cosmetic composition prepared by fermenting a complex extract of citron, bellflower, and arrowroot, which are natural products, and a method for preparing the same.

Recently, not only has social interest in beauty continuously increased, but aesthetic factors are recognized as an important social competitiveness, and the desire to maintain a more youthful and beautiful appearance is also strong, so the demand and interest in functional cosmetics continues to increase. is currently doing.

According to the Korean Functional Cosmetics Act, the 'functionality' of functional cosmetics was required to be effective in anti-aging, anti-wrinkle, whitening, and skin protection against UV rays. If there is, it has been changed to be recognized as having new functionality.

Among various functional cosmetics, the use of multi-functional cosmetics including whitening function is increasing every year, and as pigmentation remains the number one skin concern for Asian women, including Korea, the growth of the whitening cosmetic market is expected to be steady.

Human skin color is determined by several factors, such as the activity of melanocytes that make melanin pigment, the distribution of blood vessels, the thickness of the skin, and the presence or absence of internal and external pigments such as carotenoids and bilirubin. The most important is the black pigment called melanin, which is produced by the action of various enzymes such as rosinase.

Melanin is a phenolic biopolymer widely distributed in nature and is a complex of black pigment and protein, and melanin synthesis around us occurs when the cut surfaces of apples, potatoes, and bananas are exposed to air. Found on the integument, feathers, skin, head, and eyes

Currently, many whitening cosmetics contain substances that can inhibit the synthesis of melanin in skin cells, but their limitations are continuously pointed out due to the strong toxicity, low stability and skin-permeability, and insufficient activity of most cosmetic compositions. .

On the other hand, since natural plant products have not only excellent safety but also various beneficial ingredients, research and development is required to find plant natural products that have both excellent whitening effect and safety for human skin.

Korean Patent Publication No. 10-2015-0139688

It is an object of the present invention to provide a functional cosmetic composition for skin whitening and skin moisturizing comprising a mixed fermented extract of citron, bellflower and arrowroot.

Another object of the present invention is to provide a method for preparing a functional cosmetic composition comprising mixing extracts of citron, bellflower and arrowroot, and fermenting the extract.

One aspect of the present invention provides a functional cosmetic composition for skin whitening and skin moisturizing comprising a mixed fermented product of a citron extract, a bellflower extract, and an extract of arrowroot.

In the present invention, the "citron" is a kind of citrus produced in Northeast Asia, including Korea, China, and Japan, and the Korean one has the strongest flavor and the thick skin. The major production areas in Korea are Goheung, Wando, Jangheung, Jindo, and Jeju Island in Jeollanam-do. Citron contains 3 times more vitamin C than lemon, so it is good for colds and skin care, contains a lot of organic acids that prevent aging and fatigue, and contains more vitamin B, sugar and protein than other citrus fruits It contains protective flavonoids and is known to prevent cerebrovascular disorders and wind.

In the present invention, citron may be used as a whole citron fruit, or only the citron peel, seeds or pulp may be used separately. Specifically, in the present invention, the extract can be prepared using the whole citron fruit, but is not limited thereto.

In the present invention, the "bellflower ( Platycodon grandiflorum )" is also called Gilgyeong (桔梗) in oriental medicine, and is a perennial plant belonging to the Chorongaceae, which is native to China, Japan, etc. including Korea. Bellflower is a herbal medicine that has been used for respiratory diseases such as drainage, expectoration, bronchitis, and asthma, and various pharmacological actions are known. For example, among the pharmacological components of bellflower, triterpenoid saponin has antitussive, expectorant, central nervous suppression (sedation, analgesic, antipyretic effect), anti-inflammatory action for acute and chronic inflammation, and anti-ulcer action. and gastric juice secretion inhibitory action, anticholinergic action of lowering blood pressure by dilating blood vessels, blood sugar lowering action, cholesterol metabolism improvement action, and the like. In addition, bellflower extract inhibits fungal afratoxin, and inulin fraction is known to have phagocytosis and strong antitumor efficacy against solid cancer and ascites cancer.

In the present invention, the "arrowhead ( pueraria thunbergiana Benth )" is a perennial deciduous broadleaf plant belonging to the legume family, geographically distributed in Japan, China, Taiwan, and Manchuria in Northeast Asia, and naturalized and distributed in North America. There are 10 species distributed worldwide, and one species is inhabited in Korea. About 16 to 19% of the root of arrowroot is composed of starch, and contains physiologically active substances such as isoflavones, oligosaccharides, peptides, phytic acid, dietary fiber, plant sterols, phenols, and saponins. is widely used as As for the physiological activities of arrowroot, it is effective in preventing deterioration of liver function such as antioxidant function, sultoxin removal and liver protection, and is known to have antipyretic action, blood pressure and heartbeat strengthening action, etc. In addition, it is reported to contain a large amount of estrogenic substances effective in the treatment of osteoporosis. In the present invention, it is possible to use only brown powder, which is sediment that settles after grinding arrowroot, but is not limited thereto.

The “extract” refers to a solvent in which the active ingredient contained in the extraction raw material is transferred by contacting the solvent and the extraction raw material under specific conditions. All can be included regardless. For example, extracting a component soluble in a solvent from a natural product using water or an organic solvent, a specific component of a natural product, for example, extracting only a specific component such as oil, etc. may be included. In addition, extracts of citron, bellflower, or arrowroot, diluted or concentrated solutions of the extracts, dried products obtained by drying the extracts, preparations or purified products of the extracts, or mixtures thereof, etc., all formulations that can be formed using the extract itself and the extract may contain an extract of Furthermore, the extract may be extracted from natural, hybrid or varietal plants, and may also be extracted from plant tissue cultures.

The method of extracting the extract of the present invention can be extracted according to a method commonly used in the art. Non-limiting examples of the extraction method include cold extraction, hot water extraction, ultrasonic extraction, filtration, reflux extraction, extrusion extraction, and the like, and these may be performed alone or in combination of two or more methods.

The type of the extraction solvent used in the present invention is not particularly limited, and any solvent known in the art may be used as needed. Non-limiting examples of the extraction solvent include water; C1-C4 lower alcohols, such as methanol, ethanol, propyl alcohol, and butyl alcohol; polyhydric alcohols such as glycerin, butylene glycol and propylene glycol; and hydrocarbon solvents such as methyl acetate, ethyl acetate, acetone, benzene, hexane, diethyl ether, and dichloromethane; Alternatively, a mixture thereof may be used, and specifically, water, lower alcohol, 1,3-butylene glycol, and ethyl acetate may be used alone or in a mixture of two or more. In addition, the extract extracted as described above is filtered to remove floating solid particles, for example, filtered using nylon or the like, or filtered using freeze filtration, etc. It can be used after drying.

In the present invention, the term “fermentation” refers to a process in which microorganisms decompose organic matter using enzymes. It is known that the effect of the raw material that has undergone the fermentation process by the microorganisms is increased at least 2 times to up to several tens of times compared to before fermentation. Fermented metabolites contain a large amount of amino acids, organic acids and antioxidants that are beneficial to the skin, thus promoting skin metabolism and making the skin texture elastic and smooth. In addition, during the fermentation process, particles become smaller and toxins such as heavy metals are decomposed to increase absorption, and skin problems or allergic side effects can be alleviated.

The "fermented extract" may be prepared by diluting the extract in a solvent, specifically purified water, to a certain concentration, inoculating the microorganism for fermentation and fermenting.

The mixed fermented extract of citron, bellflower and arrowroot can increase the skin-beautifying effects such as skin whitening, moisturizing and antioxidant effects by reducing the physiologically active substances in the fermentation process or interacting with the newly created fermented products, and is safe in the body as well However, the skin penetration ability can be significantly improved.

In the present invention, the fermented extract may be a fermented extract obtained by inoculating a strain of Bifidobacterium spp. in an extract of citron extract, bellflower extract and arrowroot.

The Bifidobacterium spp . strain mainly uses oligosaccharides containing aminosaccharides and inhibits the growth of harmful bacteria in the intestine to promote digestion and absorption of proteins, synthesize vitamins, and provide them to the body, wherein the Bifidobacterium spp. Ferment extract promotes the conversion of profilagrin, a precursor of natural moisturizing factor (NMF), into a natural moisturizing factor, thereby improving skin moisture and providing moisture and radiance to the skin. In addition, the rich polysaccharide component contained in the ferment extract can suppress the evaporation of moisture within the skin surface.

The Bifidobacterium sp may be a Bifidobacterium breather bracket (Bifidobacterium breve), Bifidobacterium bipyridinium bushes (Bifidobacterium bifidum), Bifidobacterium ronggeom (Bifidobacterium longum), or Bifidobacterium inpeon tooth (Bifidobacterium infantis) However, it may be preferably Bifidobacterium bifidum .

The present inventors confirmed through a specific experiment that the above strain can significantly improve the skin whitening effect by effectively converting the active ingredients of the mixed extract of citron, bellflower and arrowroot and increasing the whitening active ingredient.

In the present invention, the term “skin whitening” refers to suppressing the production of various biomolecules such as melanin, oxidation-reduction hemoglobin, carotene, and melanoids that affect skin color, thereby alleviating skin pigmentation phenomena such as blemishes, freckles, and melasma. It refers to the effect of improving skin brightness and uniformity by alleviating yellowishness and redness.Specifically, in the present invention, skin whitening is used to refer to the effect of increasing the brightness by brightening the skin tone, but is not limited thereto.

In the present invention, the term “skin moisturizing” refers to the effect of improving the moisture retention of the skin, and in order to determine the skin moisturizing effect, the moisture content before and after spraying the cosmetic composition of the present invention was measured using a skin oil and moisture meter.

In the present invention, the cosmetic composition comprises a softening lotion, a nourishing lotion, a moisture cream, a nourishing cream, a massage cream, an essence, an ampoule, a gel, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a mist, a spray and a powder. It can be formulated with one or more selected from. Specifically, the cosmetic composition may be formulated in the form of a mist for skin whitening and moisturizing, but is not limited thereto.

The cosmetic composition may be formulated by a conventional method. In the formulation of external skin preparations, reference may be made to the contents disclosed in Remington's Pharmaceutical Science, Mack Publishing Company, Easton PA, and in the formulation of cosmetic compositions, the International cosmetic ingredient dictionary, 6th ed (The cosmetic, Toiletry and Fragrance Association) , Inc., Washington, 1995) may be referred to.

Specifically, the cosmetic composition can be prepared in general emulsified formulations and solubilized formulations. For example, lotion, such as a softening lotion or a nourishing lotion; emulsions such as facial lotions and body lotions; creams such as nourishing creams, moisturizing creams, and eye creams; essence; makeup ointment; spray; gel; pack; sunscreen; makeup base; foundations such as liquid type, solid type or spray type; powder; makeup removers such as cleansing creams, cleansing lotions, and cleansing oils; Alternatively, it may be formulated as a cleaning agent such as a cleansing foam, soap, body wash, but is not limited thereto. In addition, the cosmetic composition may be formulated as an ointment, patch, gel, cream or spray, but is not limited thereto.

In the cosmetic composition, in addition to the essential components in each formulation, other components may be appropriately blended within the range that does not impair the purpose according to the present invention, depending on the type or purpose of use, etc. of the formulation.

The cosmetic composition may include a conventionally acceptable carrier, for example, oil, water, surfactant, humectant, lower alcohol, thickener, chelating agent, colorant, preservative, fragrance, etc. may be appropriately mixed, but is limited thereto no.

The acceptable carrier may vary depending on the formulation. For example, when formulated into an ointment, paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide or their Mixtures can be used

When the cosmetic composition is formulated as a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder, or mixtures thereof may be used as a carrier component, and in the case of a spray, chlorofluorohydro It may further contain a propellant such as carbon, propane, butane or dimethyl ether.

When the cosmetic composition is formulated as a solution or emulsion, a solvent, solubilizer, or emulsifier may be used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl benzoate, propylene glycol, 1,3-Butylglycol oil may be used, and in particular, cottonseed oil, peanut oil, corn germ oil, olive oil, castor oil and sesame oil, glycerol fatty esters, fatty acid esters of polyethylene glycol or sorbitan may be used.

When the cosmetic composition is formulated as a suspension, a liquid diluent such as water, ethanol or propylene glycol as a carrier component, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester; Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracanth and the like can be used.

When the cosmetic composition is formulated as a soap, alkali metal salts of fatty acids, fatty acid hemiester salts, fatty acid protein hydrolysates, isethionates, lanolin derivatives, aliphatic alcohols, vegetable oils, glycerol, sugars, etc. can be used

The cosmetic composition may contain fatty substances, organic solvents, solubilizers, thickeners, gelling agents, softeners, antioxidants, suspending agents, stabilizers, foaming agents, and fragrances commonly used in the industry depending on the quality or function of the final product. , surfactants, water, ionic or nonionic emulsifiers, fillers, sequestering agents, chelating agents, preservatives, blocking agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, commonly used in cosmetics It may additionally contain adjuvants commonly used in cosmetic or dermatological fields, such as any other ingredients.

However, the adjuvant and its mixing ratio may be appropriately selected so as not to affect the desirable properties of the cosmetic composition according to the present invention.

Another aspect of the present invention comprises the steps of: a) grinding 20 to 40 parts by weight of citron and 3 to 7 parts by weight of bellflower to mix the pulverized product;

b) grinding 15 to 25 parts by weight of arrowroot, mixing the pulverized product of step a) with the settled sediment (brown powder), and mixing and pulverizing 10 to 20 parts by weight of water to obtain a mixed pulverized product; and

c) heating the mixed pulverized product at 90 to 100 ° C for 30 minutes to 2 hours, and pressing with a hydraulic press to obtain a mixed extract; provides a method for producing a mixed extract of citron, bellflower and arrowroot .

Specifically, in step a), citron may have 30 parts by weight, bellflower may have 5 parts by weight, and in step b), arrowroot may have 20 parts by weight and water may have 13 parts by weight, but is not limited thereto.

In addition, specifically, the heating temperature in step c) may be 95 °C, and the heating time may be 1 hour, but is not limited thereto.

In addition, the present invention, in addition to the steps a) to c), d) mixing the extract with Bifidobacterium bifidum ( Bifidobacterium bifidum ) and fermenting at 20 to 30 ° C. for 3 to 5 days; further It provides a method for producing a mixed fermented extract of citron, bellflower and arrowroot containing.

Specifically, the fermentation temperature in step d) may be 25° C., but is not limited thereto.

In addition, specifically, the fermentation period in step d) may be fermentation for 4 days, but is not limited thereto.

In an embodiment of the present invention, a mixed extract of citron, bellflower, and arrowroot was prepared using 30Kg of citron, 5Kg of bellflower, 20Kg of arrowroot and 13Kg of water, and it can be modified and applied as needed.

The functional cosmetic composition of the present invention is manufactured by fermenting a complex extract of citron, bellflower, and arrowroot, which is a natural product, has no cytotoxicity and thus has less skin irritation, and is low molecular weight through fermentation or synergistic skin of various fermented products that were not in the original extract. It has a cosmetic effect and is characterized by having excellent whitening, skin regeneration and moisturizing effects.

In addition, the composition can be usefully used as a natural functional cosmetic composition because it has excellent fragrance and lasts for a long time.

It should be understood that the effects of the present invention are not limited to the above-described effects, and include all effects that can be inferred from the configuration of the invention described in the detailed description or claims of the present invention.

Hereinafter, the present invention will be described in detail by way of Examples. However, the following examples are merely illustrative of the present invention, and the present invention is not limited by the following examples.

Example 1. Preparation of mixed extract of citron, bellflower and arrowroot

Prior to preparing the mixed extract of citron, bellflower and arrowroot, all materials were prepared in a state in which water washing and alcohol washing were completed in order to remove contamination such as foreign substances and mold.

A pulverized product was obtained by pulverizing 30 Kg of citron and 5 Kg of bellflower washed through the above process. After crushing the washed arrowroot 20 kg in water, the supernatant except for the sediment (brown powder) that settled down was discarded, and only the settled brown powder was used.

A mixed pulverized product was obtained by mixing the pulverized material of citron, bellflower, and arrowroot and 13 kg of water, and repeating pulverization 5 to 10 times using a large pulverizer.

The mixed and pulverized product was placed in a water bath, heated at 95° C. for 1 hour, and the compressed product obtained by pressing with a hydraulic press was filtered several times through a sieve to obtain a mixed extract.

Example 2. Preparation of mixed fermented extract of yuzu, bellflower and arrowroot

Bifidobacterium bifidum was mixed with the mixed extract of citron, bellflower and arrowroot obtained in Example 1 and fermented at 25° C. for 4 days to prepare a mixed fermented extract of citron, bellflower and arrowroot.

Comparative Example 1. Preparation of mixed extract of yuzu and bellflower

A mixed extract of citron and bellflower was prepared in the same manner as in Example 1, except that arrowroot was not mixed.

Comparative Example 2. Preparation of mixed fermented extract of yuzu and bellflower

In the same manner as in Example 2, Bifidobacterium bifidum was mixed with the mixed extract obtained in Comparative Example 1 and fermented at 25° C. for 4 days to prepare a mixed fermented extract of citron and bellflower.

Experimental Example 1. Experiment to confirm the whitening effect of mixed fermented extracts of citron, bellflower and arrowroot

In order to confirm the whitening effect of the mixed fermented extract of the present invention, a human application experiment was performed on the compositions of Examples and Comparative Examples.

The whitening effect was confirmed for 45 men and women in their 30s and 50s for 3 weeks, and the compositions of Examples and Comparative Examples were sprayed on the back of the hand with a sprayer to measure the degree of improvement in pigmentation and skin tone (brightness).

Specifically, the 45 males and females were divided into 4 groups of 9 people, and the compositions of Examples 1 and 2 and Comparative Examples 1 and 2 were sprayed on the back of the hand 3 times daily for 3 weeks for each group, and the 9 control group was whitening commercially available. Mist (containing niacinamide) was sprayed 3 times daily for 3 weeks.

Thereafter, the difference in skin tone (brightness) of the skin after 3 weeks was measured using a colorimeter to determine the whitening effect. At this time, prior to skin tone measurement, all test subjects washed their hands thoroughly and recorded the average value measured three times, and the color difference meter was measured using a commercially available portable color difference meter (TES-135A).

The colorimeter displays colors using the L*a*b* colorimetric system, and in this experiment, it was determined whether the skin tone was improved using the L* value (brightness) as an index. That is, since the L* value represents from black (L*=0) to white (L*=100), the value obtained by subtracting the first L* value from the L* value after 3 weeks is the change in skin brightness (ΔL*) , and the average value of Δ after 3 weeks for each group was obtained and shown in Table 1.

experimental composition Change value of brightness (ΔL*) Example 1 12.2 Example 2 18.3 Comparative Example 1 9.7 Comparative Example 2 14.6 control 17.8

As shown in Table 1, when extracts of yuzu, bellflower and arrowroot were fermented (Example 2 and Comparative Example 2), the whitening effect was superior to that of non-fermented (Example 1 and Comparative Example 1). It was confirmed to have a more excellent whitening effect when additionally included. Furthermore, the mixed fermented extract of citron, bellflower and arrowroot of Example 2 of the present invention has a ΔL* value of 18.3, similar to a whitening mist containing niacinamide, a component approved by the Ministry of Food and Drug Safety (ΔL*=17.8) It was confirmed that the skin whitening effect of the composition of the present invention was very excellent by showing a more excellent whitening effect.

Experimental Example 2. Experiment to confirm moisturizing effect of mixed fermented extract of citron, bellflower and arrowroot

In order to test the moisturizing effect of the mixed fermented extract of the present invention, after washing hands for 20 men and women in their 30s and 50s, dry them sufficiently for 10 minutes, and spray purified water (control group) and compositions according to Examples and Comparative Examples on the back of the hand to 15 The moisture of the skin was measured every minute. The moisture content of the skin was measured using a commercially available skin oil and moisture meter (daom-609).

Moisture content (%) was expressed by subtracting “value before spraying” from “value after spraying”, and the experimental conditions were set at room temperature of 25°C and relative humidity of 25%. In order to obtain an objective result, each subject was measured three times, and the average value of each is shown in Table 2.

hours (minutes) 15 minutes 30 minutes 45 minutes 60 minutes Example 1 63.5 53.6 44.6 32.7 Example 2 65.8 56.2 45.8 35.2 Comparative Example 1 60.5 49.8 37.4 25.6 Comparative Example 2 61.7 50.1 39.5 26.7 Control (purified water) 9 3 0 0

As shown in Table 2 above, both the Examples and Comparative Examples of the present invention showed significantly superior effects compared to the control sprayed with purified water, but the Example containing arrowroot showed excellent moisturizing effect compared to the comparative example that did not include arrowroot. was confirmed to be indicated. Furthermore, it was confirmed that the composition of Example 2, which had undergone the fermentation process, had a very good moisturizing effect as it was confirmed that the moisture content was 35% or more even after 60 minutes had elapsed. Experimental Example 3. Overall preference measurement of mist formulation

For the overall preference when each composition prepared in Examples and Comparative Examples was used as a mist formulation, 24 adults in their 20s to 60s were selected and sensory tests were performed.

The overall preference of the mist formulation containing the composition of the present invention was measured as a sensory test for oily feeling, absorption power, moisturizing power, stickiness, flexibility, odor, odor lasting power, odor strength, and overall feeling of use. For the rating, a 10-point rating method was used, and the higher the preference, the higher the score. The results are shown in Table 3 below.

item Example 1 Example 2 Comparative Example 1 Comparative Example 2 oily feeling 8.3 8.2 7.8 7.7 absorptivity 8.3 8 7.5 7.3 moisturizing power 8.5 9.3 8.1 8.3 stickiness 9.4 9.2 8.8 8.6 dreadlocks 9.0 7.6 9.1 8.0 Persistence of scent 8.8 8.1 9 8.2 intensity of odor 8.4 7.5 8.5 7.6 overall feeling of use 8.6 8.3 8.4 7.9

As shown in Table 3, it was confirmed that all of the Examples and Comparative Examples of the present invention showed a high satisfaction level of about 8 points when used as a mist formulation. Specifically, it was confirmed that Examples 1 and 2 containing arrowroot showed a high level of satisfaction related to moisturizing power, while the preference for fragrance slightly decreased.

In addition, in the case of Example 2 and Comparative Example 2 including the fermentation process, the preference related to the fragrance decreased and the overall feeling of use was low, but it was confirmed that, nevertheless, overall high satisfaction was exhibited.

In other words, it was confirmed that, although the brown powder of arrowroot is effective in moisturizing the skin, the preference may decrease due to the characteristic scent, and the preference related to the fragrance may be lowered overall because the characteristic fragrance is generated during the fermentation process.

To summarize these results, when the mixed fermented extract of citron, bellflower, and arrowroot of the present invention is used as a cosmetic in a mist formulation, even if it includes the fermentation process and brown arrowroot powder (Example 2), It was confirmed that the preference did not decrease significantly, and thus it could have excellent application potential as a cosmetic.

The above description of the present invention is for illustration, and those of ordinary skill in the art to which the present invention pertains can understand that it can be easily modified into other specific forms without changing the technical spirit or essential features of the present invention. will be. Therefore, it should be understood that the embodiments described above are illustrative in all respects and not restrictive. For example, each component described as a single type may be implemented in a dispersed form, and likewise components described as distributed may be implemented in a combined form.

The scope of the present invention is indicated by the following claims, and all changes or modifications derived from the meaning and scope of the claims and their equivalents should be construed as being included in the scope of the present invention.

Claims (6)

A functional cosmetic composition for skin whitening and skin moisturizing, comprising a mixed fermented product of citron extract, bellflower extract and arrowroot extract. According to claim 1,
The fermentation is a functional cosmetic composition that is fermented using Bifidobacterium bifidum. According to claim 1,
Skin whitening and moisturizing mist composition comprising the functional cosmetic composition. a) grinding 20 to 40 parts by weight of citron and 3 to 7 parts by weight of bellflower to mix the pulverized product;
b) grinding 15 to 25 parts by weight of arrowroot in water, mixing the pulverized product of step a) with the settled sediment (brown powder), and mixing and pulverizing 10 to 20 parts by weight of water to obtain a mixed pulverized product; and
c) heating the mixed pulverized product at 90 to 100° C. for 30 minutes to 2 hours, and compressing it with a hydraulic press to obtain a mixed extract; A method for producing a mixed extract of citron, bellflower and arrowroot. 5. The method of claim 4,
d) mixing the extract with Bifidobacterium bifidum and fermenting the mixture at 20 to 30° C. for 3 to 5 days; a method for producing a mixed fermented extract of citron, bellflower and arrowroot further comprising. 6. The method of claim 5,
The fermentation period is 4 days, the method for producing a mixed fermented extract of citron, bellflower and arrowroot.