KR20130027227A - Citrus flower compositions for improving skin conditions - Google Patents

Abstract

PURPOSE: A cosmetic composition for improving skin condition using citrus flowers is provided to ensure sedation, to protect skin from oxidative materials, and to enhance anti-aging. CONSTITUTION: A cosmetic composition for improving skin condition contains a dried citrus flowers or a citrus flower extract as an active ingredient. An antioxidative composition contains the citrus flower extract as an active ingredient. A composition for suppressing oxidative stress contains the citrus flower extract as an active ingredient. The cosmetic composition is manufactured in the form of a skin, lotion, a cream, an essence, a serum, a foundation, a pack, a soap, cleansers, a shampoo, a rinse, a hair tonic, a patch, and a mist.

Description

Citrus flower compositions for improving skin conditions

The present invention relates to a cosmetic composition for improving skin conditions comprising an extract obtained from citrus flowers of Jeju-do.

In the name of citrus fruits, citrus fruits, citrus fruits, citrus fruits, etc., are used interchangeably. However, if you divide them, citrus fruits are collectively referred to as citrus. Citrus fruits are native to Southeast Asia, including India, but varieties have been improved and cultivated in China, Europe, the United States, and Japan. In Korea, according to Chungam Kim Jong-ui in 1512, one of the treasures of Jeju is tangerine, and it is recorded that there are nine kinds (kumquat, oil, blueberry, dongjeongguk, sugar-dried, potato, wild mandarin orange, red mandarin orange, tangerine). . However, the current citrus cultivars of Korean citrus varieties are almost disappeared, and the predominantly cold-sealed citrus persimmons, which were introduced in Japan in 1911, are the dominant species. Citrus from Jeju has good taste and aroma, has a sweet scent, and contains various vitamins and other physiologically active ingredients. Recently, citrus fruit production is increasing rapidly in Korea, and artificially fallen citrus flowers to control the yield every year. In foreign countries, extracts are extracted from orange flowers in Italy, France, etc. and used and exported as cosmetic fragrances or spices for various foods. As it is used, its use range is limited. Accordingly, the present invention intends to use citrus flowers directly or in the form of extracts using a special technique or in the form of flower water obtained by a new method. Citrus petals, extracts, or flower water produced by these various recipes contain more active ingredients than extracts or flower water produced by conventional methods, showing excellent effects in various biological areas such as skin soothing, moisturizing, whitening, or antioxidant activities. In addition to its efficacy, it has a sweet scent and can give a refreshing and refreshing feeling. In addition, by utilizing the citrus flowers waste resources can be expected to recycle resources.

The skin is an organ that exists at the outermost part of the body. It is an important organ that has various functions and has a great influence on appearance and image.It is composed of various cells and unique structures, covering the outer surface of the body to prevent evaporation of moisture It can be said that it acts as a body's primary defense against external environment such as body temperature control and ultraviolet rays.

Human skin color is determined by the concentration and distribution of melanin in the skin. Melanin pigment produced by melanocytes of human skin is a phenolic polymer material having a complex form of black pigment and protein, and plays an important role in blocking skin damage caused by ultraviolet rays. In melanin biosynthesis, the action of tyrosinase in melanocytes is reported to be the most important. And dopaquinone plays a key role in the skin blackening process.

The human body contains about 72% water, which keeps the skin moist and softly protects it. Therefore, the maintenance of water in the skin is a very important factor to have healthy skin and will be the most basic factor to cope with the weakening of the resistance over time. Moisture is properly maintained in the stratum corneum to maintain the elasticity and flexibility of the skin, normal skin contains about 30% water or water-soluble components.

However, due to the stimulation caused by changes in the environment and life patterns, skin stress caused by various stresses and environmental pollution caused by social life, the moisture of the stratum corneum decreases, so that the skin dries and the surface becomes rough and irritated. Can easily get damaged. Reactive oxygen occurs rapidly in these processes, and damage to cellular proteins, lipids, and nucleic acids due to skin damage caused by oxide causes hypertension, atherosclerosis, hyperlipidemia, diabetes, cancer, rheumatoid arthritis, and nerve damage. It is not only considered to be an important pathogenesis in sexual diseases, but also as a major factor in the aging process. Skin damage immediately leads to damage of the stratum corneum, which is the outermost layer of the skin, through which the loss of transdermal moisture increases skin dryness, which is accompanied by abnormal sebum secretion by a sudden sebum secretion to prevent this, Or, conversely, too intelligent. These various complex skin problems can finally be exacerbated by skin itching, erythema, tissue damage, and the like. Thus, by appropriately treating the above series of steps, skin damage can be minimized. In order to prevent this phenomenon and maintain a healthier and more beautiful skin, bioactive substances obtained from various known animals, plants, and microorganisms are added to cosmetics to maintain the unique function of the skin and to activate skin cells. Efforts have been made to effectively improve skin conditions.

For this reason, there is a need for the development of a substance that can be easily used in medicine, cosmetics, food, etc. as a natural substance that is stable to the human body having antioxidant capacity, soothing effect, moisturizing effect, and whitening effect.

Under this background, the present invention can be used as a cosmetic composition for improving or improving skin condition by providing a skin protection effect on an oxide and having a whitening effect and imparting soothing and moisturizing properties to the skin.

An object of the present invention is a cosmetic composition comprising citrus flower extract as an active ingredient, which has a whitening effect on the skin and gives moisturizing and soothing effects to maintain healthy skin and protect the skin from oxides, thereby preventing skin aging. The present invention provides a composition for improving skin condition.

In the present invention, the skin external preparation for improving the skin condition comprising the citrus flower extract as an active ingredient, the above objects and advantages will be apparent by the following examples and claims.

The composition comprising the citrus petal water extract according to the present invention can be used as a composition for improving skin as well as maintaining a healthy skin by exhibiting a soothing, moisturizing effect and an antioxidant effect.

In order to achieve the object as described above, the cosmetic composition for skin whitening, soothing, moisturizing, antioxidant power enhancement according to the present invention is characterized by containing a citrus flower or a natural extract.

The present inventors have tried to develop a natural plant or plant extract excellent in product stability and safe to use without side effects on the skin and excellent in skin whitening, moisturizing, soothing and antioxidant effects. As a result, it was confirmed that citrus flower water extracted from citrus flowers can give skin condition improvement effect by showing excellent whitening effect, moisturizing effect, soothing effect and antioxidant effect.

Citrus flower production method of the present invention is as follows.

Citrus Flower Harvest-Screening-Washing-Drying

Drying in citrus flowers is not limited to natural drying, vacuum oven drying or hot air drying.

Citrus blossom water production method of the present invention is as follows.

1) Put dried citrus flower into cryogenic liquid nitrogen about 3 to 5 times by weight, and grind completely with propeller rotating at high speed from 3000rpm to 10000rpm for 1-10 minutes with a commonly used brander or mixer. In order to completely grind during the grinding process, liquid nitrogen is injected in multiples of 2 times at intervals of 2 minutes. After grinding, it is left at room temperature for about 10 minutes, and the water of the vesicles constituting the petal plant cells flows out in a state where the petals are physically completely broken to obtain a completely red thick flake petal.

2) This petal crushed product is filtered through 1 um filter paper to obtain a filtrate. This filtrate was named Citrus Petal Stock Solution.

3) Extract the cell lysate on the filter paper from which the petal water stock solution was filtered by cold immersion method using water as a solvent. After extracting by soaking for 3 days at room temperature using water containing 2% of the natural preservative ingredient provided in the prior registration patent (No. 10-0782599) as a solvent and filtered to obtain a citrus petal extract. Named it.

The citrus petals obtained in paragraph 2 were found to be 17% by weight of the total dry weight of the petals. In addition, the citrus petal extract obtained in 3) was found to be about 7% of the dry weight of the petals. Of course, it was confirmed that the content increased by about 1 to 5% by adjusting the immersion time.

The citrus flower or citrus flower water thus obtained was made into a conventional cosmetic composition to evaluate skin improvement effects such as skin whitening, moisturizing, soothing, and antioxidant effects.

Was born whitening and moisturizing jump out of this efficacy, can be suppressed in the case of citrus kkotsu constant moisturizing and closely related hyaluronic Ronnie Days (Hyaluronidase) activity of the enzyme in the skin level, and the synthesis of hyaluronic acid (hyaluronic acid) It has been found to be able to enhance. In addition, the moisturizing power of the human body showed a high degree of moisturizing. Dried citrus petals can be used in packs, baths and various applications. When used as citrus petals packs, the whitening and moisturizing effects were excellent.

The composition of the present invention exhibits an excellent antioxidant effect by having a cytoprotective effect from oxide. Antioxidant compositions of the present invention can prevent or damage the skin damage from the oxide material can inhibit or prevent a variety of diseases or diseases that can be attributed to the oxide material. Preferably, the antioxidant composition of the present invention can be applied to the prevention or suppression of skin aging.

In addition, the composition of the present invention has a skin whitening purpose. As used herein, the term whitening refers to the action of improving skin trouble that prevents blackening of the skin caused by the deposition of melanin pigment.

The skin whitening composition of the present invention exhibits an excellent whitening effect of inhibiting melanin production by inhibiting the activity of tyrosinase, and has high stability and almost no side effects such as skin irritation.

Therefore, the camellia petals or camellia petals obtained in the present invention can be prepared with various kinds of cosmetic compositions containing suitable additives according to the use and method.

Although the content of this invention is demonstrated to the following implementation, the content of this invention is not limited to this.

Example 1. Citrus petals  Preparation of Stock

500 g of dried citrus fruit raw materials are charged with 2 liters of cryogenic liquid nitrogen, and then thoroughly ground in a mixer at 10,000 rpm for 6 minutes. In order to completely grind during the grinding process, 500 ml of liquid nitrogen was added at two minute intervals. After grinding, if left at room temperature for 10 minutes, the petals are physically completely broken. As a result, water from the vacuoles constituting the petal plant cells flowed out to obtain a thick red flake of petals. The obtained petal crush was filtered through 1 m filter paper to obtain a filtrate, which was named as citrus petal stock solution. The dry weight of the citrus petal stock solution was 70 g.

Example 2. Citrus petals  Preparation of extract

70g of the petal water stock solution was extracted by soaking for 3 days at room temperature using water containing 2% of the natural preservative ingredient provided in the prior registration patent (No. 10-0782599) as a solvent, followed by filtration to obtain a citrus petal extract. . The citrus petal extract was concentrated under reduced pressure at 7 degrees in a distillation apparatus equipped with a cooling capacitor to obtain an extract of 23 g (dry weight).

Example  3: skin improvement Cosmetics  Preparation of the composition

Nutrition Cream

Examples of the preparation of nutritional creams containing the extracts of Examples 1 and 2 are as shown in Table 1 below. Purified water, triethanolamine, and propylene glycol, which were aqueous phases, were dissolved by heating at 70 ° C. The oily fatty acid, an oily component, an emulsifier, and the preservative were melt | dissolved by adding the liquid which heated and melted at 70 degreeC here. After the emulsification was completed, the solution was cooled to 45 ° C., followed by adding and dispersing centella and magnolia extract and flavor, and then cooling to 30 ° C.

Components and Contents of Nutritional Cream Containing Extract ingredient Content (% by weight) Example 1 Extract, Example 2 Extract 0.1 Liquid paraffin 5.0 Cetylaryl alcohol 1.5 Polyglyceryl-3 Methyl Glucose Distearate 1.5 Glyceryl stearate 0.5 Squalane 2.0 Propylene glycol 2.0 glycerin 6.0 Triethanolamine 0.2 Carboxy Vinyl Polymer 0.2 Tocopheryl acetate 0.3 Preservative, incense a very small amount Purified water Balance Sum 100

Comparative manufacturing example: Purified water  Cream containing

In the preparation example of the nutrition cream containing the extract, the composition of the cream replaced with purified water without the extract is as follows.

ingredient Content (% by weight) Jojoba oil 4.0 Liquid paraffin 5.0 Cetylaryl alcohol 1.5 Polyglyceryl-3 Methyl Glucose Distearate 1.5 Glyceryl stearate 0.5 Squalane 2.0 Propylene glycol 2.0 glycerin 6.0 Triethanolamine 0.2 Carboxy Vinyl Polymer 0.2 Tocopheryl acetate 0.3 Preservative, incense a very small amount Purified water Balance Sum 100

Example 4 Moisturizing Test of Human Body of Citrus Flowers

The degree of transepidermal water loss (TEWL) was measured using a TEWA (Tewameter TM210, Germany) evaporator. The measurement was performed by treating the cream containing each extract prepared above and the cream containing no sample at all and measuring the degree of moisturizing once after 4 weeks, and statistically compared. All measurements were taken in a constant temperature and humidity room with no air movement, no direct sunlight, and a room temperature of 22 ° C and a relative humidity of 40%. As shown in Table 3, when the citrus flowers of the comparative example were not contained, the degree of water loss reduction was insignificant. On the other hand, when containing the citrus flowers of the embodiment, it was found that the moisturizing effect is excellent.

TEWL change (unit: g / ㎡ / h) Type of pack Test embroidery Before processing After processing Mean ± mean error Mean ± SD Untreated group 30 23.85 ± 2.42 22.18 ± 3.56 Comparative example 30 22.76 ± 3.15 19.52 ± 3.48 Example 30 23.84 ± 3.43 10.65 ± 2.25

Example 5 Whitening Effect Test of Citrus Flowers on Human Body

In order to verify the direct whitening effect of citrus flowers on the skin, brightness was measured using a chromameter (Minolta, chromameter CR-300) according to the visual inspection method. Sunlight 0.86 J / m ² at the upper part of the arm once a day for 3 days, and then creams containing citrus and non-creams twice a day for 15 days for 20 to 30 males and 15 females. The degree of disappearance of pigmentation was calculated by measuring the lightness before coating and after coating.

[Equation 1]

Whitening effect (%) = (brightness value before application-brightness value after application / brightness value before application) X 100

As shown in Table 4, when the citrus flower of the comparative example is not contained, the whitening effect is hardly observed, whereas when the citrus flower of the example is contained, the whitening effect is excellent.

division Whitening Effect (%) Untreated group 1.5% Comparative example 15.2 Example 65.4

Example 6. Bathing efficacy test of citrus flowers

In order to test the bathing efficacy of citrus flowers, 40 participants (20 males and 20 females) were surveyed for skin soothing and moisturizing effects.

After bathing, fill the bathtub with water, administer 15g of dried citrus petals, and after 5-10 minutes, begin to bathe for 10 ~ 20 minutes when significant ingredients begin to emerge, and the temperature of the bath is maintained at 38-40 degrees. The survey was conducted three times after bathing.

The age-specific number of subjects showing satisfaction with skin moisturizing effect, sedative effect and fatigue recovery, and the number of satisfied subjects compared with respondents were expressed as the effective ratio.

Moisturizing effect Fatigue Recovery Sedation gender Male (20) Male (20) Female (20) Female (20) Male (20) Female (20) 20's 3 3 3 4 3 3 30s 4 4 4 4 4 3 40 spaces 4 3 4 4 3 4 50s 3 3 3 3 3 3 Satisfied Subjects / Respondents 27/40 29/40 26/40 Effectiveness rate 67.5% 72.5% 65%

Moisturizing, soothing effect only by bathing three times using citrus flowers as shown in Table 5 above

It can be seen that there is a significant effect on fatigue recovery.

Example 7 Measurement of Melanin Inhibition Effect

B16 melanoma cells were used to measure the inhibitory effect of melanin production by citrus blossoms and compared with the inhibitory effect of melanin production by arbutin known to inhibit melanogenesis.

In this experiment, B16 melanoma cells were inoculated in DMEM medium containing 10% fetal bovine serum (FBS) in 6-well microplates at 1 × 10 ⁵ per well, and at 5% CO ₂ and 37 ° C, Incubate until at least 80% attachment. The medium was then removed and the sample was replaced with medium diluted to the appropriate concentration and incubated at 5% CO ₂ and 37 ° C. for 3 days. The concentration range of citrus blossoms was determined to be 1 ppm, 10 ppm and 100 ppm without cytotoxicity. The cells from which the medium was removed were washed with PBS (phosphate buffered saline) and treated with trypsin to recover the cells. The recovered cells were measured by using a hematocytometer to measure the cell number, centrifuged at 5,000 to 10,000 rpm for 10 minutes, and the supernatant was removed to obtain a precipitate. The cell precipitate was dried at 60 ° C., and 100 ml of 1 M sodium hydroxide solution containing 10% DMSO was added to obtain intracellular melanin in a 60 ° C. thermostat. The absorbance was measured at a wavelength of 490 nm to determine the amount of melanin per cell.

sample Treatment Concentration (ppm) Melanin production inhibition rate (%) Arbutin 50 30 Citrus One 2 10 16 50 35

As shown in Table 6, it can be seen that citrus blossom water at the treatment concentration has a higher melanin inhibition rate than arbutin. In addition, it can be seen that the melanin production inhibitory activity of citrus blossoms is concentration-dependent.

Example 8. On citrus blossom  by Intracellular Tairosineiz ( tyrosinase ) Activity Inhibition Effect Measurement

Rat pigment cells (B16 melanoma cells) were used to measure the inhibitory effect of melanin production by citrus blossoms and compared it with the inhibitory effect of intracellular tyrosinase activity by arbutin known to inhibit melanin production.

In this experiment, B16 melanoma cells were inoculated with DMEM medium containing 10% of FBS (fetal bovine serum) in 6-well microplates at 1 × 10 ⁵ per well, and the cells were placed at the bottom of the well at 5% CO ₂ and 37 ° C. Incubated until at least about 80% adhered. After incubation, the medium was removed, the sample was replaced with a medium diluted to an appropriate concentration, and then incubated at 5% CO ₂ , 37 ° C for 3 days. The concentration range of citrus blossoms was determined to be 1 ppm, 5 ppm and 10 ppm without cytotoxicity. The cells from which the medium was removed were washed with PBS (phosphate buffer saline) and treated with trypsin to recover the cells. The recovered cells were measured by using a hematocytometer (hematocytometer) and then centrifuged at 5,000 to 10,000 rpm for 10 minutes, and then the supernatant was removed to obtain a precipitate. The cell precipitate was pulverized using lysis buffer and then centrifuged at 12,000 rpm for 10 minutes to collect supernatant. This solution was measured for absorbance at 492 nm wavelength and cell constant allowance tyrosinase activity was determined. The results are shown in Table 7 below.

sample Treatment concentration (ppm) Inhibition rate of intracellular tyrosinase activity (%) Arbutin 5 40
Citrus
One 10 5 32 10 45

Example 9. Hyaluronidays  Activity inhibition test

100 μl of the hyaluronic acid-agarose mixture warmed to 55 ° C. was dispensed on a 96-well microplate and gelated by standing at room temperature. 100 μl hyaluronidase (40 unit) was added thereto, and cultured at 37 ° C., and then the supernatant was discarded. 100 μl of 10% (w / v) cetylpyridinium chloride was added thereto and left at room temperature for 30 minutes, and the absorbance was measured at 595 nm. The results are shown in Table 8.

sample Treatment concentration (ppm) Hyaluronidase Activity (%) Untreated group 0 100%
Citrus One 95 10 50.5 20 32.4

Hyaluronidase is an enzyme that hydrolyzes hyaluronic acid. Hyaluronic acid is a constituent substance present in the extracellular matrix of skin or cartilage tissue. It is responsible for space-filling, structure-stabilizing, and cell-coating, and is an important substance in the moisturizing function of skin. Known. In particular, hyaluronic acid is a substance that can contain 200 times more moisture than its own. The reduction of this substance in the skin may cause the skin to dry. In this experiment, the effect of citrus flower water on hyaluronidase activity was observed. Observation results showed that citrus blossom water treatment group significantly inhibited hyaluronidase activity compared to the untreated group.

Example 10. Hyaluronic Acid Synthesis Enhancement Test

After treating the sample extract with 10ppm for 24 hours to human normal fibroblast cells, the cells were finally lysed to obtain a cell sample. The concentration of hyaluronic acid was measured using a Hyaluronan Enzyme-linked Immunosorbent Assay (ELISA) kit (Echelon Biosciences Inc., UT, U.S.A.). The results are shown in Table 9 below.

sample Treatment concentration (ppm) Hyaluronic Acid Concentration (? G / ml) Untreated group 0 4.86
Citrus One 6.21 10 19.64 20 25.56

As shown in Table 9, it can be seen that citrus blossom water promotes hyaluronic acid biosynthesis to a higher level than the untreated group.

Example 11. Citrus  Safety test for human skin

A cumulative skin irritation test was conducted to verify the safety of citrus blossoms on human skin.

By diluting the extracted citrus blossoms with purified water at 10 ppm, 20 ppm, and 50 ppm concentrations, the citrus fruits were subjected to a total of 9 24-hour cumulative patching on the upper forearm every other day for 30 healthy adults. Whether the number of flowers irritated the skin was measured.

Pinnacle method (Finn chamber, Epitest Ltd, Finland) was used. 15 µl of each of the external preparations for skin was added dropwise to the chamber, followed by patching. The degree of response to the skin each time was scored using Equation 2 below, and the results are shown in Table 10 below.

&Quot; (2) "

Average Responsiveness = [[{(Response X Responsiveness) / (Total Number of Subjects X Highest Score (4 points))}] X 100] ÷ Number of Tests (9)

In the reactivity, ± (a) is 1 point, + (b) is 2 points, and ++ (c) is 4 points, and when the average reactivity is less than 3, it is determined as a safe composition.

exam
matter Number of subjects who responded Average
Reactivity 1 week 2 weeks 3 weeks Primary Secondary Third Primary Secondary Third Primary Secondary Third a b c a b c a b c a b c a b c a b c a b c a b c a b c Control group
(Purified water) 0 - - --- --- --- --- --- One - - --- --- 0.09 Citrus
Flower 10ppm 0 - - --- --- --- --- --- --- --- --- 0.00 20 ppm 0 - - --- --- --- --- --- --- --- --- 0.00 50 ppm 0 - - --- --- --- --- --- One - - --- --- 0.09

As shown in Table 10, citrus blossoms were found to have no irritation to human skin.

Example 12. Antioxidant Effect

Superoxide dismutase (SOD) is known as an antioxidant enzyme that converts superoxide anions into H ₂ O ₂ and O ₂ . This experiment is a method of evaluating the antioxidant capacity of a sample by observing the rate at which the superoxide anion generated by Xanthine oxidase was removed by the sample used in the test. The experiment was carried out using a kit purchased from Dojindo (Japan) (SOD Assay Kit-WST), and was tested according to the manufacturer's protocol. In brief, the effect was determined by observing the removal rate of superoxide anion by the sample, the presence or absence of antioxidant function. The experimental results are summarized in Table 11.

sample Treatment Concentration (ppm) Superoxide Anion Removal Rate (%) Citrus One 5 10 14 20 32

As shown in Table 11, it can be seen that citrus blossoms of the present invention have an antioxidant effect in a concentration-dependent manner.

Example 13. Oxidative  Cytoprotective effect against stress

The cytoprotective effect of citrus blossoms against oxidative stress using epidermal human fibroblasts was measured by comparing cell viability.

This study was a skin fibroblast cells in the well bottom under 10% of FBS (fetal bovine serum) is in a DMEM medium was inoculated to 12-well microplate 5 × 10 ⁴ pieces per well 5% CO _2, 37 ℃ Incubated until at least about 80% adhered. After incubation, the medium was removed, the sample was replaced with a medium diluted to an appropriate concentration, and then incubated for 2 days by treatment with H ₂ O ₂ after pretreatment at 5% CO ₂ , 37 ° C. for 1 hour. The concentration range of citrus blossoms was determined to be 10 ppm, 20 ppm and 100 ppm without cytotoxicity. The cells from which the medium was removed were washed with PBS (phosphate buffer saline) and replaced with DMEM medium containing 1% MTT ((3- [4, 5-dimethylthiazol-2-yl] -2, 5-diphenyl tetrazolium bromide). After 3 hours, the supernatant was dissolved in DMSO, and the absorbance was measured at a wavelength of 540 nm.

Oxidative stress sample Treatment concentration (ppm) Cell survival rate (%) No treatment No treatment No treatment 100 H ₂ O ₂
process No treatment No treatment 56 Citrus 10 67 20 69 50 71

As shown in Table 12, it can be seen that citrus blossoms have a protective effect on H ₂ O ₂ treatment.

Claims (11)

Cosmetic composition for improving skin condition comprising dried citrus flower or citrus flower extract as an active ingredient According to claim 1, citrus fruits are Wenzhou mandarin, Hallabong, Jeju lemon, kumquat, bottle tangerine, tangerine tangerine, red tangerine, mandarin orange, blue mandarin orange, cinnamon tangerine, inchang mandarin orange, young mandarin orange, mandarin orange, cheonhyehyang, jinjihyang, hearing, paragraph, sugar yuja When generically citrus such as dead skin, semi-nut, etc. Antioxidant composition comprising citrus flower extract as an active ingredient Oxidative stress inhibitory composition comprising citrus flower extract as an active ingredient According to claim 1, wherein the condition of the skin improvement is characterized in that the skin whitening, true information moisture Cosmetic composition containing citrus saline solution as an active ingredient to prevent drying of the skin or scalp or for additional moisturizing The composition according to any one of claims 1 to 5, wherein the composition is a cosmetic composition. 8. The composition of claim 7, wherein the composition has one or more formulations in the group consisting of skin, lotion, cream, essence, serum, foundation, pack, soap, cleanser, shampoo, rinse, hair tonic, patch, mist. Characteristic composition The method according to claim 8, wherein the stock solution is used in an amount of 0.01% to 10% based on the composition. The method of claim 8 wherein the citrus flower extract is used from 10% to 100% The method of claim 8 used in the form of dried petals, petals or petal fragments.