KR100563547B1 - A cosmetic composition containing extract of Lespedeza bicolor turczaninow var and/or Solanum nigrum L - Google Patents

Abstract

The present invention relates to a cosmetic composition containing a plant extract as a main active ingredient, the cosmetic composition of the present invention by containing at least one selected from the extract of Prunus quinquefolia or extract from the crow as the main active ingredient excellent whitening effect and anti-inflammatory skin irritation It provides a mitigating effect.

The extract of Prunus japonica or Corvus alba according to the present invention inhibits the synthesis of a signaling substance related to melanin synthesis, which is a substance causing blemishes, freckles and skin pigmentation, thereby suppressing melanin synthesis and exhibiting a whitening effect. It inhibits the skin cell death caused by the cosmetic substrate, including the skin irritation and immune enhancing effect through the inhibition of inflammation-related enzyme activity.

Therefore, it is possible to prepare a cosmetic composition such as a lotion, cream, milky lotion, pack, powder and the like having excellent skin whitening effect, anti-inflammatory, skin irritation effect by containing the extracts of the barberry and horse.

Prunus vulgaris extract, black berry extract, whitening, skin irritation relief.

Description

A cosmetic composition containing extract of Lespedeza bicolor turczaninow var and / or Solanum nigrum L} as a main active ingredient

The present invention relates to a cosmetic composition containing the plant extract as the main active ingredient.

Human skin is weak or severely irritated in response to ultraviolet light, ozone from environmental pollution or other harmful substances present in the atmosphere. In particular, the skin is susceptible to damage by oxygen radicals and nonspecific proteases released by stimulation.

The factors that determine the color of the skin are basically the partial or total pigmentation such as tanning caused by blemishes, freckles and UV exposure, excluding the differences according to race, region, sex and age, and the distribution of acne, scars and keratin. Blood circulation, stress, and health. Among the above factors, the most important factor is pigmentation.

However, excessive pigmentation is a serious mental burden from the perspective of skin beauty, which impedes normal social activities. Since ancient times, Asian women have preferred white and fair skin, and this has been the standard of beauty. Research to identify and prevent this has been continued.

The pigments that affect skin color include melanin, melazoids, carotene, and hemoglobin, the most important of which is melanin, and the biggest factor affecting melanin biosynthesis is ultraviolet and hormone secretion. Melanin plays an important role in absorbing or scattering ultraviolet rays to prevent damage to the skin from ultraviolet rays. There is no special maximum absorption wavelength and it absorbs light in all areas. In addition, it is excellent in removing the active oxygen species, but sometimes melanin itself generates free radicals, and other substances are reduced or oxidized by catechol or quinone in the melanin structure, and melanin itself shows the properties of free radicals. Pray.

The biosynthesis of melanin is a series of oxidation processes, starting with the conversion of tyrosine, an amino acid, from the melanocytes of melanocytes to tyrocinase and dihydroxy phenylalanine, followed by brown (pheomelanin) and black (eumelanin). It is formed of a polymer of). This biosynthesis process is carried out in a special type of brown intracellular organelles called melanosomes. Melanosomes, including melanin granules, move from the periphery of the nucleus to the dendritic end, and then into the cytoplasm by the phagocytosis of keratinocytes. Accumulate around the nucleus of the site.

The synthesis of melanin, the number of melanosomes, and the movement to surrounding keratinocytes are partially affected by hormones and ultraviolet rays, and primarily by genetic effects. In addition, cytokines, such as cytokines, copper, zinc, and iron, which are involved in the expression of tyrosinase, the synthesis and transfer of melanin, and metal ions such as interferon, prostaglandin, and histamine are known to be involved. Society of Cosmetic Scientists, 25: 77-127, 1999).

Cytokines are molecules involved in biological actions that send signals between cells, and their categories vary widely. Most are proteins or peptides, and sugars are attached. Most are small molecular weight proteins (8-25 kDa), consisting of a single chain. Cytokines regulate all important biological actions such as morphogenesis, cell growth, cell activity, inflammation, immunity, tissue repair, fibrosis (fiber formation), and the like. Examples of cytokines include various interleukins, interferons, growth factors, and the like.

According to Imokawa et al., UVB irradiation in human keratinocytes is associated with significant production of interleukin-1a (IL-1a) and interleukin-6 (IL-6). It is known that overexpression of genes can be observed. In addition, IL-1a and IL-6 induce the expression of endothelin-1 (ET-1) in keratinocytes, which are known to act on adjacent melanocytes and play an important role in melanin biosynthesis. (G Imokawa, et al . : J. Biol. Chem. , 267: 24675-24680, 1992, G Imokawa, et al .: Pigment Cell Res. , 10: 218-228, 1997). Therefore, by suppressing the expression of cytokines such as IL-1a and IL-6, the whitening effect can be expected by eliminating the cause of more fundamental pigmentation.

Ascorbic acid (JP-A 4-9320), hydroquinone (JP-A 6-192062), kojic acid (JP-A-56-7710), arbutin (JP-A 4-93150), and some extracts are already tyrosinase. It is used as a whitening cosmetic because it has an inhibitory activity, but its stability in cosmetic formulations is poorly degraded and colored, or its use is limited due to the occurrence of off-flavor, efficacy at the biological level, unclear effect and safety problems.

In addition, consumers are increasingly demanding the use of cosmetics with a minimum of side effects such as skin protection, irritation suppression, inflammation suppression, sun protection, etc., while having high quality functional properties according to a special composition. In addition to the whitening effect, cosmetics can not satisfy the multifunctional aspect of skin irritation.

The present invention has been made in order to solve the above problems, an object of the present invention is selected from one or more natural products selected from the extract of Prunus bark, or Dec. By selecting the extracted extract, confirming its efficacy as a cosmetic raw material and developing a method of using the same, increasing the selection range of various cosmetic raw materials, and using this raw material to produce cosmetics having a predetermined functional effect without further stimulation. It is to provide a method that can be produced.

That is, an object of the present invention is to inhibit melanin synthesis, which is a substance causing blemishes, freckles, and skin pigmentation, to have a whitening effect, and to inhibit skin cell death, to reduce skin irritation caused by cosmetic substrates, and to induce inflammation. It is to provide a natural extract showing a skin irritation-releasing effect and immune enhancing effect such as related enzyme activity inhibitory effect.

Still another object of the present invention is to provide a cosmetic composition such as a lotion, cream, milky lotion, pack, powder, etc., which has excellent effects such as skin whitening effect, anti-inflammatory, and skin irritation effect by containing extracts of Prunus chinensis and Crow.

The present invention for achieving the above object provides a cosmetic composition containing the main ingredient as an ingredient selected from the group consisting of Prunus chinensis extract, black rind extract, and mixtures thereof.

Barberry is a deciduous shrub of the dicotyledonous plant Rosaceae, whose name is Lespedeza bicolor turczaninow var. Commonly growing in mountains and fields. Chin leaves are thin and long, dark brown, about 5mm long. Small leaves are egg-shaped or upside down. The outer side is dark green with snowy hair on the back and flat edges. Flowers bloom reddish purple in July-August. The calyx is shallowly divided into 4, the rear one is divided into 2 again, and the end is pointed. In oriental medicine, it was used for various skin diseases such as athlete's foot, eczema and dry ringworm.

Camellia is the annual year of the branch family, the scientific name is Solanum nigrum L. It grows on the roadside or in the lowland of the lowlands. It is 20 ~ 90cm high, and branches are spread a lot, and some ridges appear on the main stem. Leaves are regenerated, egg-shaped, bottom or blunt, original or broad bottomed. It flows to the upper part of the long leaf, 6-10cm long, 4-6cm wide, with no cradle or superficial. Flowers bloom in May-July, 6-7mm in diameter, white, inflorescences come out from the leaves, and flowers of 1 ~ 3cm in length are split, flowers with 3 ~ 8mm in length, and 7-12mm in length are in the shape of mountains. Run Calyx and corolla split into 5 and spread laterally, with 1 pistil and 5 stamens. Capsules are round, 6-7mm in diameter, ripen in black. In oriental medicine, it was used for gynecological diseases, hemorrhoids, severe wounds, and chronic bronchitis.

Such natural plants have not previously been known to have a whitening effect, and furthermore, there was no example of applying the extract of the natural plant for the whitening effect, and it shows the skin whitening, anti-inflammatory and skin irritation effect through the present invention. Will be revealed.

The extract of Prunus chinensis or Corvus chinensis used in the present invention is a leachate obtained by leaching or transferring lychee from Apricot or Corica, or a concentrate obtained by partially or totally concentrating the leachate, or an extract extracted by drying the concentrate again. And the chemical substance itself having the main effect contained in the extract.

In addition, it is preferable that the content of Prunus chinensis extract or the extract of Camellia according to the present invention or a mixture thereof is 0.001 to 30.0 wt% based on the total lyophilized weight of the cosmetic composition. When the content of the extract is less than 0.001% by weight, there is almost no skin improvement effect, and when the content of the extract is more than 30.0% by weight, it is not economical because the degree of increase of the effect on the content is insignificant.

In addition, the cosmetic composition according to the present invention may contain, in addition to the above-mentioned extract, other ingredients that can give a synergistic effect to the main effect, preferably within the range of not impairing the main effect of the present invention.

The birch extract or the extract in the yam or a mixture thereof may be present in the composition at 0.001 to 30.0% by weight based on the total lyophilized weight. If the plant extract is present in the form of a solution containing the extract, those skilled in the art will be able to adjust the amount of this solution in the composition according to the invention within the concentration range.

The cosmetic composition of the present invention uses a solvent selected from purified water, methanol, ethanol, glycerin, ethyl acetate, butylene glycol, propylene glycol, dichloromethane, hexane and mixtures thereof.

Also subject of the present invention is the use of cosmetic compositions used as skin whitening agents, anti-inflammatory agents, skin irritants.

The compositions of the present invention may be in the form of an aqueous, aqueous-alcoholic or oily solution, oil-in-water or oily water or multiple emulsions, aqueous or oily gels, liquid, pasty or solid anhydrous products, oil dispersions in aqueous phase using globules. And more preferably in the form of lipid vesicles in ionic and / or nonionic form.

The composition may be somewhat fluid and may have the appearance of a white or colored cream, ointment, milk lotion, serum, essence, paste or mousse. It may optionally be applied to the skin in the form of an aerosol or may be in the form of a solid, such as a stick. It may be used as a skin care product and / or makeup product.

In a known manner, the compositions according to the invention are also useful in conventional cosmetics such as hydrophilic or lipophilic gelling agents, hydrophilic or lipophilic active agents, preservatives, antioxidants, solvents, fragrances, fillers, blockers, pigments, odorants and dyes. It may contain. The amounts of these various adjuvants are amounts commonly used in the art and are, for example, from 0.01% to 20% by weight relative to the total weight of the composition. Depending on their nature, such adjuvants may be incorporated into the fatty phase, aqueous phase, lipid vesicles and / or nanoparticles. In any case, the adjuvant and its proportions will be chosen so as not to adversely affect the desirable properties of the cosmetic composition according to the invention.

The invention will now be illustrated by the following examples. However, these Examples are only illustrative description of the present invention and the scope of the present invention is not limited to these Examples.

Example 1 Preparation of Hawthorn Extract

The dried bark was refluxed three times for 5 hours with 95% (V / V) ethanol aqueous solution, and the mixture was cooled and then filtered through Whatman # 5 filter paper. The filtered extract was concentrated under reduced pressure and lyophilized at 50 ° C or lower. The birch extract was prepared by dispersing / dissolving the depressurized concentrate and the lyophilised product using at least one solvent selected from purified water, ethanol, butylene glycol, and propylene glycol.

Example 2: Preparation of extract in black yam

The dried and dried dried black buckwheat was refluxed three times for 5 hours with 95% (V / V) ethanol aqueous solution, and then cooled and filtered through Whatman # 5 filter paper. The filtered extract was concentrated under reduced pressure and lyophilized at 50 ° C or lower. Extracts were prepared by dispersing / dissolving at least one solvent selected from purified water, ethanol, butylene glycol, and propylene glycol such that the vacuum concentrate and the lyophilisate were contained in an amount of 0.001 to 30.0% by weight.

Example 3 Experiment for Determining Melanogenesis Inhibition Using B16F1 Melanogenic Cells

This Example is to evaluate the whitening effect by looking at the degree of inhibition of melanin production on B16F1 melanocytes in order to confirm the whitening effect of the extract of Prunus chinensis and the extract from black yam obtained in Examples 1 to 2.

B16F1 melanocytes used in this example are cell strains derived from mice, and cells that secrete a black pigment called melanin. Samples were treated during the artificial culture of these cells to evaluate the degree of melanin black pigment reduction. B16F1 melanocytes used in this example were used by receiving from the ATCC (American Type Culture Collection, Accession No .: 6323).

The melanin biosynthesis inhibitory effect of B16F1 melanogenesis cells was measured as follows.

B16F1 melanocytes were divided into 6 well plates at a concentration of 2 X 10 ⁶ per well, and the cells were attached and cultured for 72 hours by treating the samples at a concentration that does not cause toxicity. After 72 hours of incubation, the cells were detached with trypsin-EDTA, the cell number was measured, and the cells were recovered by centrifugation. Quantification of intracellular melanin was carried out with a slight modification of the method of Rotan: Cancer Res. , 40: 3345-3350, 1980. The cell pellet was washed once with PBS, and then 1 ml of homogenization buffer solution (50 mM sodium phosphate, pH 6.8, 1% Triton X-100, 2 mM PMSF) was added and vortexed for 5 minutes to disrupt cells. Melt the extracted melanin by adding 1N NaOH (10% DMSO) to the cell filtrate obtained by centrifugation (3,000 rpm, 10 minutes), and measure the absorbance of melanin at 405 nm with a microplate reader. Production inhibition rate (%) was measured. Melanin inhibition rate (%) of B16F1 melanogenesis cells was calculated according to Equation 1, IC ₅₀ value is the concentration of the substance inhibiting 50% melanogenesis.

% Inhibition = [(A-B) / A] X 100

A: Melanin amount in wells without sample

B: Melanin amount in the wells to which the sample was added

Inhibition of melanin production of B16F1 melanogenesis cells showed an IC ₅₀ value of 0.1%, and an IC ₅₀ value of the extract of C. japonica was 0.15%. It showed a similar or superior effect as compared to the extract of the lettuce (Table 1).

 Sample Name  Treatment concentration (%) Inhibitory effect of melanin synthesis (IC ₅₀ )  Pear Tree Extract (Example 1)  0.1  0.1%  Extract of Blackberry (Example 2)  0.1  0.15%  Hydroquinone  0.1  0.03%  Arbutin  0.1  0.2%  Lettuce extract  0.1  5%  Soluble Licorice Extract  0.1  0.03%

Example 4 Measurement of Inhibitory Effect of Cell Signaling Agents Related to Melanin Synthesis Using Keratinocytes

This example targets the interleukin-1α and interleukin-6, which are substances which promote the secretion of keratinocytes by the UVB from the bark extracts and the extracts of the quince extracts obtained in Examples 1 to 2 to promote melanin formation of melanocytes. It is to confirm that it does. Inhibiting the activity of interleukin-1α and interleukin-6 also inhibits their melanin synthesis. This is a method for evaluating a whitening agent by inhibiting melanin synthesis by ultraviolet rays in skin exposed to external ultraviolet rays in daily life.

In this example, HaCaT (Boukamp P., et al., The Journal of Cell Biology , 106: 761-771, 1988) was used as a keratinocyte cell line isolated from human skin. The cytokine inhibitory effect of the cell signal transduction agent was measured as follows.

HaCaT was dispensed into 24 well plates at a concentration of 1 × 10 ⁵ per well and cells were attached for one day. The next day, the cell culture medium was removed and washed with PBS to irradiate the cells with 6.8 mJ UVB to promote cytokine secretion, and 10 ppm of extracts of Prunus chinensis and Black Root were treated to the cells and incubated for 5 hours. At this time, bovine serum was not added to the cell culture medium used. After 5 hours, 150 μl of cell culture medium was placed in a 96 well plate and coated at 4 ° C. for one day. The medium was removed, washed three times with PBS-T (PBS with 0.2% Tween20), and then treated with blocking buffer (dissolved in 3% Bovine Serum Albumin in PBS-T) for two hours. The buffer was removed and the primary antibodies, anti-IL-1α and anti-IL-6, were diluted and treated with blocking buffers, respectively. After reacting at 37 ° C. for 90 minutes, the plate was washed three times, and then treated at 37 ° C. for 90 minutes after treatment with the anti-lebitt-IgG, which is a secondary antibody. After washing five times, the substrate reaction solution (833 mM sodium citrate, 385 mM sodium phosphate, 0.1% H ₂ O ₂ , 0.0005% O-phenylenediamine) is added to 150ul per well and reacted for 40 minutes at 37 ℃. 50 μl of 2NH ₂ SO ₄ was added per well to stop the reaction, and the absorbance was measured at 490 nm.

% Inhibition = [(A-B) / A × 100]

A: absorbance of wells after treatment with UVB without treatment

B: absorbance of wells treated with samples after UVB treatment

As a result of measuring the inhibitory effect of cytokine synthesis of HaCaT keratinocytes, IC ₅₀ values of P. japonica extract and C. japonica extract were 0.005-0.01% for interleukin-1α and 0.001-0.008% for interleukin-6. It showed a superior effect compared to phosphorus arbutin (Table 2).

As a result, in the case of the extract of Prunus chinensis and Corvus of the present invention, unlike the conventional whitening agent, which merely inhibits the activity of tyrosinase enzyme, the whitening effect is inhibited by inhibiting the synthesis of cell signaling material involved in melanin biosynthesis. It can be seen that.

          sample Cytokine Synthesis Inhibitory Effect (IC ₅₀ )  Interleukin-1α  Interleukin-6  Arbutin  0  0  Birch Extract  0.01%  0.008%  Black berry extract  0.005%  0.001%

Example 5 Experiment for Determining Inhibitory Effect of Inflammation-Related Enzyme (hyaluronidase) Activity

This Example is determined by measuring the enzyme activity of hyaluroniadiaze, an inflammation-associated enzyme, hyaluroniadiasis, in order to confirm the anti-inflammatory effects of the extract of Prunus chinensis and the extract from Crow.

Hyaluronidase is an enzyme that hydrolyzes hyaluronic acid and has a function of causing inflammation. In this example, the anti-inflammatory effect was determined by applying a method (Kakegawa Y., Japanese J. of Inflammantion , 4: 437-438, 1984) to inhibit the activity of this enzyme to measure the anti-inflammatory effect.

The inhibitory effect of hyaluronidiase activity was investigated using comfrey, seesaw, triticale, oil-soluble licorice extract, Prunella vulgaris, and extracts from black yam as samples.

Inhibitory activity of hyaluronidiases of each sample was performed as follows.

100 μl of sample and 50 μl of hyaluronidiase solution (type IV-S, Sigma, 400 U / ml) were added and reacted at 37 ° C. for 20 minutes, followed by enzyme activation solution (compound 48/80 CaCl 2 ?? 2H 2 O, Sigma). Inc., 0.1 mg / ml) was added to 100 µl and reacted again at 37 ° C. for 20 minutes. 250 μl of hyaluronic acid solution (0.4 mg / ml) was added and reacted at 37 ° C. for 40 minutes. 100 μl of 0.4N NaOH was added to terminate the reaction. 100 μl of potassium borate solution was added thereto, reacted at 95 ° C. for 3 minutes, cooled, and 3 ml of ρ-dimethylaminobenzaldehyde solution was added thereto. Absorbance was measured at 585 nm to determine the inhibition rate for hyaluronidiases. Inhibition rate (%) for hyaluronidiases was calculated by the equation (3), IC ₅₀ value is the concentration of a substance that inhibits the hyaluronidiase enzyme activity by 50%.

% Inhibition = [(A-B) / A] × 100

A: Enzyme activity of wells without sample

B: Enzyme Activity of Well Added Samples

As a result of testing the inhibitory effect of hyaluroniidase enzyme, IC ₅₀ values of P. japonica extract and C. japonica extracts were 0.08 to 0.1%, which is a useful anti-inflammatory agent, a useful licorice extract and a comfrey extract, which are known to be excellent in inhibiting hyaluronidase. Compared to the three hundred seconds extract, seesaw extract and the like or showed an excellent effect (Table 3).

              Sample Name Melanin Synthesis Inhibitory Effect (IC ₅₀ )  Birch Extract  0.1%  Black berry extract  0.08%  Comfrey Extract  0.22%  Seesaw extract  0.58%  Soluble Licorice Extract  0.08%  Triticale extract  0.3%

Example 6 Evaluation of Stimulation Relaxation Effect Using Cell Culture Technology

This Example is a sodium lauryl sulfate (Sodium Lauryl Sulfate; SLS) that is a substance that causes skin irritation by using a cell culture technology in order to evaluate the stimulating relaxation effect of the extracts of Prunus chinensis and the extracts from the yam obtained in Examples 1 to 2 The effect of stimulating relaxation was evaluated to the extent of inhibiting cell death by).

SLS is a substance that is used as a criterion for evaluation of skin irritation based on cosmetics. It is bound to cell membranes to inhibit cell metabolism and destroys cell membranes to cause skin irritation.

This example evaluates the effect of reducing the cell death by SLS when SLS, fern, and horsetail extract were simultaneously added to human fibroblasts.

The Hs68 fibroblasts used in this example were used as a dermal dermal cell in humans, sold at ATCC (American Type Culture Collection, Accession No. 1635).

Stimulation relaxation evaluation experiment using the cell culture technology was carried out as follows.

Human-derived fibroblasts were dispensed in 96-well plates at a concentration of 1 × 10 ⁵ per well and incubated for 24 hours, followed by 0.01% SLS alone, 0.01% SLS and 0.01% (w / w) extracts of Prunus japonica and black yam respectively. Treated and further incubated for 24 hours. After incubation, MTT (Sigma) reagent was added and incubated for 4 hours, the culture solution was removed, 1N NaOH / isopropanol solution was added and stirred for 20 minutes, and the absorbance was measured at 565 nm using a microplate reader. The effect of the extract on inhibiting apoptosis by SLS was measured.

As a result, it was found that the extract of Prunus japonica and the extract in the black yam are irritant substances that reduce skin irritation that can occur when the cosmetic base is prescribed with the cosmetic base by inhibiting apoptosis induced by SLS (Table 4).

                sample         Fibroblast survival rate (%)  0.01% SLS  25  0.01% SLS + 0.01% Birch Extract  57  0.01% SLS + 0.01% extract in black yam  54

Examples 7 to 8 and Comparative Example 1

The present Example was prepared by the cosmetics containing the extract of Prunus chinensis and the extract from the black horse obtained in Examples 1 and 2 to evaluate the skin whitening effect in Comparative Experiment with Comparative Example 1.

The cosmetics used in the comparative experiments are in the form of a cream, the composition of which is shown in Table 5. First, the phase b) recorded in Table 5 is heated and stored at 70 ° C. To this, a) phase is added, and after pre-emulsification, it is emulsified uniformly with a homomixer and then cooled slowly to prepare a cream (Example 7, 8, Comparative Example 1). For 20 experimenters (20-35 year old female), the cream prepared in Examples 7 to 8 on the right side of the face and the cream prepared in Comparative Example 1 on the left side of the face for 2 consecutive days Applied. After completion of the test, the color of the applied areas on both sides of the face was measured using an image analyzer and the color of the skin using chromameter (Minolta CR300) to measure the change in brightness of the color (ΔL). Subjective visual observation was performed and the effect was measured according to the following classification. The results are shown in Table 6 below. At this time, the degree of whitening efficacy was classified into the following seven ratings.

Whitening efficacy evaluation criteria:

-3: very worse, -2: worse, -1: slightly worse,

 0: no change, 1: slightly improved, 2: improved, 3: greatly improved

As shown in Table 5, it can be seen that the whitening effect is excellent in the facial skin of the experimenter who applied the cream containing Prunus vulgaris and the extract of Crow.

                       Raw material    Example 7    Example 8    Comparative Example 1          end  Stearyl alcohol  8  8  8  Stearic acid  2  2  2  Stearic Acid Cholesterol  2  2  2  Squalane  4  4  4  2-octyldodecyl alcohol  6  6  6  Polyoxyethylene (25 mol addition) alcohol ester  3  3  3  Glyceryl monostearic acid ester  2  2  2      I  Pear Tree Extract (Example 1)  One  -  -  Extract of Black Horse (Example 2)  -  One  -  Propylene glycol  5  5  5  Quantity  Quantity  Quantity  Quantity

Note) Unit: Weight%

 Skin color change (L)     Objective evaluation of the skilled person     Subjective Evaluation of the Subject  Example 7  Example 8  Comparative Example 1  Example 7  Example 8  Comparative Example 1  Example 7  Example 8  Comparative Example 1  medium    5.32    4.82    2.03    2.9    2.3    1.9    2.7    2.2    1.7

Other examples are shown below. That is, lotion, milky lotion and essence containing the extract of Prunus chinensis and the extract from black yam obtained in Examples 1 and 2 were prepared in Examples 9-14.

The lotion, milky lotion and essence containing these extracts of hawthorn extract and black berry showed excellent effects on skin improvement such as excellent skin whitening effect and skin damage suppression or irritation relief function by ultraviolet rays. These birch extracts and extracts of black yam can be added to the formulations of the lotion, gel, water-soluble liquid, cream, essence, oil-in-water (O / W) and water-in-oil (W / O) type, which is a person skilled in the art It can be easily applied in the technical field to which it belongs, but the content of the present invention is not limited thereto.

Example 9: Preparation of a lotion containing a birch extract obtained in Example 1

To 8 g of 95% ethanol, 0.05 g of polypyrrolidone, 0.1 g of oleyl alcohol, 0.2 g of polyoxyethylene monooleate, 0.2 g of fragrance, 0.1 g of paraoxybenzoic acid methyl ester, a small amount of antioxidant and a small amount of pigment are dissolved. . 0.05 g and 4 g of glycerol extract obtained in Example 1 were dissolved in 85.33 g of purified water, and then the mixed solution was added and stirred to obtain a lotion having a skin improvement effect.

Example 10 Preparation of Lotion Containing the Extract of Black Root Obtained in Example 2

8 g of 95% ethanol, 0.05 g of polypyrrolidone, 0.1 g of oleyl alcohol, 0.2 g of polyoxyethylene monooleate, 0.2 g of fragrance, 0.1 g of paraoxybenzoic acid methyl ester, a small amount of antioxidant and a small amount of pigment do. 0.05 g of the extract obtained in Example 2, 5 g of glycerine was dissolved in 85.33 g of purified water, and then the mixed solution was added and stirred to obtain a lotion having a skin improvement effect.

Example 11 Preparation of Latex Containing Botanica Extract Obtained in Example 1

1.2 g of cetyl alcohol, 10 g of squalane, 2 g of petrolatum, 0.2 g of paraoxybenzoic acid ethyl ester, 1 g of glycerin monoesteralide, 1 g of polyoxyethylene (20 mole added) monooleate and 0.1 g of fragrance were mixed and dissolved at 70 ° C. , 0.5 g of the cedar extract obtained in Example 1, 5 g of dipropylene glycol, 2 g of polyethylene glycol-1500, 0.2 g of triethanolamine, and 76.2 g of purified water were dissolved by heating to 75 ° C. Both mixtures were emulsified and cooled to obtain an emulsion having an oil-in-water (O / W) type skin improvement effect.

Example 12 Preparation of an Milky Milk Containing an Extract of Black Root Obtained in Example 2

1.2 g of cetyl alcohol, 10 g of squalane, 2 g of petrolatum, 0.2 g of paraoxybenzoic acid ethyl ester, 1 g of glycerin monoesteralide, 1 g of polyoxyethylene (20 mole added) monooleate and 0.1 g of fragrance were mixed and dissolved at 70 ° C. , 0.5 g of the extract obtained from Example 2, 5 g of dipropylene glycol, 2 g of polyethylene glycol-1500, 0.2 g of triethanolamine, and 76.2 g of purified water were heated to 75 ° C. to dissolve it. Both mixtures were emulsified and cooled to obtain an emulsion having an oil-in-water (O / W) type skin improvement effect.

Example 13: Preparation of Beauty Solution Containing Botanica Extract Obtained in Example 1

5 g of 95% ethyl alcohol, 1.2 g of polyoxyethylene sorbitan monooleate, 0.3 g of kitulose, 0.2 g of sodium hyaluronate, 0.2 g of vitamin E-acetate, 0.2 g of sodium licorice, 0.1 g of paraoxybenzoic acid ethyl ester, 1 g of the birch extract obtained in Example 1 and an appropriate amount of pigments were mixed to obtain a essence having a skin improvement effect.

Example 14 Preparation of a Cosmetic Solution Containing the Extract of Black Root Obtained in Example 2

5 g of 95% ethyl alcohol, 1.2 g of polyoxyethylene sorbitan monooleate, 0.3 g of kitulose, 0.2 g of sodium hyaluronate, 0.2 g of vitamin E-acetate, 0.2 g of sodium licorice, 0.1 g of paraoxybenzoic acid ethyl ester, In the extract obtained in Example 2, 1g of the extract and a suitable amount of pigments were mixed to obtain a cosmetic liquid having a skin improvement effect.

As described above, the present invention not only suppresses the synthesis of melanin by inhibiting the synthesis of signal transduction agents related to melanin synthesis, which is a cause of blemishes, freckles and skin pigmentation, but also exhibits a whitening effect on the cosmetic substrate. The present invention provides a cosmetic composition exhibiting a skin stimulating alleviation effect and an immune enhancing effect such as skin cell death suppressing effect, which reduces skin irritation caused by inflammation, and an enzyme activity inhibitory effect related to inflammation induction.

In the above description of the preferred embodiment of the present invention, those skilled in the art will be able to practice various modifications without departing from the gist of the present invention, and such modifications are within the scope of protection of the present invention. The protection scope of the present invention should be construed as described in the claims.

Claims (8)

A cosmetic composition for skin whitening, comprising any one of 0.001 to 30.0% by weight of Prunus chinensis extract, 0.001 to 30.0% by weight of the extract of Crow as a main active ingredient. delete delete delete delete The method of claim 1, Cosmetic composition for skin whitening, characterized in that it is used for anti-inflammatory. The method of claim 1, Cosmetic composition for skin whitening, characterized in that it is used to relieve skin irritation. The emulsion according to any one of claims 1, 6 and 7, wherein the cosmetic composition is a lotion, gel, water-soluble liquid, cream, essence, oil-in-water (O / W) or water-in-oil (W / O) type emulsion. , Cosmetic composition for skin whitening, characterized in that it has a formulation selected from the group consisting of ointments.