KR20170076147A - Composition for improving skin - Google Patents

Abstract

The present invention relates to a cosmetic composition comprising a natural protease derived from pineapple and kiwi and an Indian gooseberry extract. Proteinase and Indian gooseberry extracts derived from pineapples and kiwis promote collagen synthesis in the skin fibroblasts to improve wrinkles, improve elasticity, eliminate free radicals, and exhibit antioxidative effects. The removal of stratum corneum outside the skin Thereby enhancing the whitening effect of the skin without irritation, and exhibiting an effect of improving the skin texture due to the improvement of the skin brightness.

Description

[Composition for improving skin]

The present invention relates to a composition for improving skin such as wrinkle improving, elasticity enhancement, antioxidation, whitening, improvement of skin texture and improvement of skin texture.

Skin is the largest organ of the human body that protects our body from external stimuli. It has barrier function to prevent the internal moisture and oil components from flowing out of the skin, temperature control function, excretion function, breathing Function, and so on. The skin consists of epidermis, dermis, and subcutaneous fat layer, and the outermost skin layer functions to protect skin from external environment.

As the cells constituting the epidermal layer, there are various cells such as keratinocytes, Rangelans cells, Merkel cells, and melanocytes. Among them, keratinocytes are the majority of the cells. The keratinocytes undergo cell proliferation in the basal layer to produce a large number of skin cells, which lose their cell life as they pass through the anolyte layer, where the cellular constituents inside are excreted out of the cell. Only the relatively hard keratin, And ultimately reaches the stratum corneum of the skin. However, if there is a problem in the activity of keratinocytes, skin aging, wrinkles and dryness of the skin may occur, resulting in skin sagging, and the elasticity of the skin is lowered. Therefore, it is necessary to activate the keratinocyte which produces the constituent of the epidermal layer of the skin and to improve the function of the stratum corneum to maintain the skin healthy, moisturized and beautiful.

On the other hand, the skin is an organ in which the change in the age of the human organ is evident. The morphological change according to this age occurs in the stratum corneum, the epidermis, and the whole dermis constituting the skin. Skin microrelief, a type of skin surface, is characterized by a three-dimensional microstructure formed by fine lines and is significantly different by age and site. The skin texture is divided into primary (about 20-100 μm), secondary (about 5-40 μm), and tertiary (about 0.5 μm) lines depending on the depth of the line. It is characterized by polygons and star formation due to the formation of structures and lines (Journal of the European Academy of Dermatology and Venereology. 12: 103-114, 1999). However, as the age increases or the skin becomes damaged, the network structure of the dense fine lines collapses, and fine lines (secondary, tertiary, etc.) disappear and the primary lines become deeper and wrinkles are known to be formed (The British Journal of Dermatology. 110: 129-138, 1984, Skin research and technology. 5: 189-194, 1999). In the case of damage to the intracellular DNA by various external factors, cell death and mutation are caused, and the skin is accelerated in aging, the skin function is deteriorated and the skin becomes visually rough and the beauty is greatly reduced.

On the other hand, human skin color is largely determined by the amount of melanin, hemoglobin, carotene and the like, among which melanin plays a most important role. Melanin is a pigment present in the epidermal layer of the skin. It protects skin from ultraviolet rays, protects skin from external harmful factors such as harmful oxygen and free radicals generated in the skin . In skin tissue, melanin is synthesized to protect the body from ultraviolet rays, but melanin is synthesized by various external factors, and the stratum corneum is not smoothly removed due to stress and skin aging. As a result, if the aging keratin does not fall off normally, the skin loses its activity and remains on the surface of the skin, causing pigmentation such as spots and freckles, which darkens the skin tone. Therefore, in order to prevent such pigmentation phenomenon, it is necessary to inhibit the synthesis of melanin pigment or to remove old stratum corneum. However, in order to obtain a skin whitening effect, a method of inhibiting only a process and obtaining an effect has been generally studied, so that it has not yet exhibited excellent whitening effect. Therefore, in order to obtain a sufficient whitening effect, it is necessary to develop a component which suppresses the biosynthesis of melanin and at the same time promotes the detachment of old keratin.

On the other hand, active oxygen introduced from the outside of the living body or generated in the living body causes many problems such as promoting aging of the living body, cancer, and the like. Therefore, the development and research of antioxidants that inhibit oxidation by active oxygen have been performed. Antioxidants are widely distributed in copper and plants. Many phenolic compounds, flavonoids, tocopherols, vitamin C and selenium are known in fruits and vegetables. However, the antioxidant substances present in nature can not be expected to have practically sufficient effects in skin application. Therefore, although synthetic antioxidants having excellent antioxidant ability and low cost are widely used, their use is restricted due to safety concerns such as human side effects.

If the homeostasis of the skin is broken, various problems such as aging of the skin, pigmentation, and reduction of elasticity are caused. Therefore, there is a desperate need for the development of an active ingredient which is able to improve various skin phenomena, is safe to the living body, and more effective than the existing wrinkle improving, elasticity improving, antioxidant, whitening, Is desired.

Accordingly, the inventors of the present invention found that natural protease derived from pineapple and kiwifruit and Indian gooseberry extract promotes collagen synthesis of fibroblasts of the skin, improves wrinkles, promotes elasticity, eliminates free radicals, Thereby inhibiting the synthesis of melanin, thereby exhibiting a whitening effect, and confirming the effect of improving the skin texture due to the improvement in skin brightness, thereby completing the present invention. Accordingly, an object of the present invention is to provide a composition for improving wrinkles, elasticity, antioxidation, whitening, exfoliation and skin texture improvement comprising a pineapple, a kiwi protease and an Indian gooseberry extract as an active ingredient and a cosmetic composition .

In order to solve the above-mentioned problems, the present invention may include the following means.

The cosmetic composition for skin improvement according to an embodiment of the present invention promotes collagen synthesis of fibroblasts of the skin to improve wrinkles, improve elasticity, eliminate free radicals, exhibit antioxidative effect, remove the stratum corneum outside the skin To improve the whitening effect of the skin without irritation, to confirm the effect of improving the skin texture due to the improvement of the skin brightness, and to contain the protease derived from pineapple and kiwi and the Indian gooseberry extract as an active ingredient do.

The Indian gooseberry extract is characterized by containing 0.01 to 50.0% by weight based on the total weight of the composition.

 The pineapple and the kiwi-derived protease are characterized by containing 0.001 to 20.0% by weight based on the total weight of the composition.

 The composition is characterized by having a formulation selected from the group consisting of softening agents, nutritional lotions, nutritional creams, massage creams, essences, eye creams, cleansing creams, cleansing foams, cleansing waters, packs, sprays or powders.

The natural protease derived from pineapple and kiwi according to the present invention and the Indian gooseberry extract promote the collagen synthesis of the fibroblasts of the skin to improve the wrinkles and improve the elasticity and the antioxidative effect by eliminating the free radicals, The present invention can improve the whitening effect of the skin without irritation by promoting the removal of the stratum corneum on the side of the skin,

The cosmetic composition for skin improvement according to the present invention contains natural protease derived from pineapple or kiwifruit and Indian gooseberry extract as active ingredients to exhibit wrinkle improvement, elasticity enhancement, antioxidation, whitening, exfoliation and skin texture improving activity, The ability to permeate through the skin and be absorbed, and to improve skin regeneration, wrinkle improvement, elasticity enhancement, antioxidation, whitening, exfoliation, and skin texture improvement.

The pineapple used in the present invention grows in a range of 0 to 100 cm, and the leaves are rocket-shaped and grow coarse and long. It gradually becomes narrower at the end, and its tip is pointed. There are thorns and thorns. The front is dark gray. The peduncle comes out from the center and flowers from the end in the form of a pine cone. Fruits are 15 ~ 20cm long and 10 ~ 12cm long as gathering fruits, some of which are about 30cm long and about 17cm in diameter in large varieties. The fruit shape is cylindrical, conical, and egg-shaped. When it is ripe, it turns from orange to yellow and has a scent. High sugar content and high levels of vitamin A, vitamin C and vitamin B. Pineapple-derived proteolytic enzymes are known to have anti-inflammatory and anti-inflammatory properties, with the exception of swelling and strengthening of antibiotics. It is an enzyme that warms the skin and is used for facial massage. It helps to relieve menstrual irregularities, indigestion, each parasite, and nervous fatigue.

Kiwi is a subtropical plant that lives in the vicinity of the Yangtze River in China and has been improved in New Zealand and cultivated worldwide. The kiwi is a deciduous vine plant of the dwarf tree. The leaves are round, round, oval or inverted, with an end pointed, with saw-like sawtooth on the edge, and a dense back side. Flowers bloom in June-July, white, ripening in August-October, round ovate or cylindrical shape, and brown hairs on the surface. The fruit is called kiwi because it looks like a kiwi living in New Zealand. In Korea, it was introduced in New Zealand in 1977 and is grown mainly in the southern coast.

The scientific name of the Indian gooseberry extract, which is the active ingredient of the present invention, is Phyllanthus 　 It is a small, medium-sized deciduous arboreous tree that grows indigenously throughout India. The stem is curved, the gray, smooth bark peeled off, the pale green leaf is in the form of a feather, the lemon scent drifts, and the flower is greenish yellow. The fruit is in the shape of a ball. It is a beautiful greenish-yellow with a six-striped line of 1.5 ~ 3㎝ in diameter with a light yellowish color when ripening. Indian gooseberry fruit contains 20 times more vitamin C than oranges, and it is very stable in heat, so even if exposed to high temperatures for a long time, vitamins are hardly destroyed like freshly harvested from trees, This durability and heat resistance of vitamin C contained in Indian gooseberry is believed to be derived from the combined tannin content. Indian gooseberry fruit is regarded as a natural hair nutrient, and a lot of hair care products such as hair oil and hair conditioner containing Indian gooseberry extract are sold. It is also known that vitamin C is abundant and has excellent whitening and antioxidant effect.

 The active ingredients of the present invention, pineapple and kiwi-derived protease, are thiol-based proteolytic enzymes such as Papain, Ficin and Chymopapain, which have SH groups already known as bromelain and actididin. Belong to the same group. Bromelain extracted from pineapple is an enzyme preparation composed of four enzymes. Bromelain constitutive enzymes hydrolyze various complex proteins such as casein, gelatin, hemoglobin, and cell proteins.

The content of the protease derived from kiwifruit and pineapple is 0.001 to 20.0% by weight. When the content of the composition is less than 0.001% by weight, the skin improving effect is insignificant. When the content is more than 20.0% by weight, And it is difficult to expect the effect.

The Indian gooseberry extract is characterized by containing 0.01 to 50.0% by weight based on the total weight of the cosmetic composition. If the content of the Indian gooseberry extract is less than 0.01% by weight, the skin improvement effect is insignificant. If the content is more than 50.0% by weight, skin irritation may occur.

The active ingredients contained in the cosmetic composition of the present invention include components commonly used in cosmetic compositions, such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavorings, in addition to kiwi, pineapple-derived proteolytic enzymes and Indian gooseberry extracts. And the like.

In the present invention, the term " wrinkle-reducing effect " refers to inhibiting or inhibiting the generation of wrinkles on the skin, or alleviating already-generated wrinkles.

In the present invention, the 'elasticity-enhancing effect' refers to an increase in elasticity to the skin, which suppresses or inhibits the loss of elasticity of the skin, or alleviates the already-reduced elasticity.

In the present invention, the term 'antioxidative effect' refers to the action of free radicals or reactive oxygen species (ROS), which are highly reactive according to oxidative stress caused by intracellular metabolism or ultraviolet rays, Refers to inhibition of oxidation, and includes removal of free radical or reactive oxygen species, thereby reducing damage to the cells.

In the present invention, the whitening effect refers to the effect of inhibiting the melanin synthesis by inhibiting the melanocyte production, the blackening process of the released melanin, or inhibiting the activity of tyrosinase, or reducing the produced melanin Lt; RTI ID = 0.0 > melanin. ≪ / RTI >

In the present invention, the term "exfoliation" refers to removal of the stratum corneum outside the skin or normalization of the exfoliation period in order to remove aging dead skin.

In the present invention, the term " skin texture improving effect " refers to smoothing the skin surface, imparting gloss, and brightening the skin tone by inhibiting or inhibiting roughness of the skin surface due to aging, stress, .

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood that both the foregoing description and the following detailed description are exemplary and explanatory and are intended to be illustrative of the invention and are not intended to be exhaustive or to limit the invention to the precise forms disclosed. It will be self-evident

Hereinafter, the configuration of the present invention will be described in detail.

Experimental Example 1: Promotion of collagen synthesis

Example 1 was added to the culture medium of human-derived fibroblasts to examine the promoting effect of collagen synthesis at the cellular level. The biocompatible collagen was quantitated using a PICP EIA kit (Procollagen Type I C-Peptide Enzyme Immunoassay Kit).

Was added to a culture medium of human fibroblasts (7 x 10 ⁴ cells / cm 2) together with vitamin C and a control (no supplement) so that the final concentrations of Example 1 were 0.5% and 1%, and the cells were cultured for 1 day, The culture was taken and the degree of collagen biosynthesis at each concentration was measured with a PICP EIA Kit at 450 nm using a spectrophotometer. The collagen biosynthesis performance was calculated based on the relative performance relative to the control group, and the results are summarized in Table 1 below.

Promotion of collagen synthesis by concentration (number of repeats = 3) Additive sample Application concentration (%) Increase in collagen synthesis (%) compared to control Vitamin C 0.005% 29.6% Example 1 0.5% 39.5% 1.0% 45.1%

As a result, as shown in the results of Table 1, it can be seen that Example 1 of the present invention has excellent collagen synthesis ability for human-derived fibroblasts.

Experimental Example 2: Antioxidant effect - Free radical scavenging rate

The free radical scavenging activity was measured to confirm the antioxidative activity of Example 1. DPPH (2.2-diphenyl-1-picryl hydrezyl) radical scavenging activity was used to measure antioxidant activity. 0.4 mM DPPH (2.2-diphenyl-1-picryl hydrezyl) solution was dissolved in ethanol and filtered. The prepared DPPH solution and the sample solution were mixed at a ratio of 1: 1 and vigorously mixed. Then, the mixture was allowed to stand at room temperature for 10 minutes. After the absorbance at 515 nm was measured using a microplate reader (BioTek, USA), DPPH radical removal rate (%) was calculated by the following equation (1). At this time, 0.1 mg / ml of ascorbic acid was used as a positive control to remove radicals.

[Equation 1]

DPPH radical scavenging rate (%) = (A - B) / A x 100

Where A is the absorbance of the blank test solution and B is the absorbance of the test solution

sample Free radical scavenging rate (%) Ascorbic acid 100 ug / ml 71.2% Example 1 (1.0%) 83.7%

As can be seen from the results shown in Table 2, it can be seen that Example 1 has an excellent antioxidative effect as compared with ascorbic acid which is a known antioxidant.

Example 3: Cell proliferation effect

Keratinocyte, a skin keratinocyte, was inoculated into a DMEM medium containing 4.5 g / L glucose, 10% serum and 1% antibiotic, and cultured at 37 ° C in a 50 ml T-flask. After incubation for 24 hours under 5% CO 2, the cells were treated with 0.05% trypsin containing 0.02% EDTA, and then inoculated in a 50 ml T-flask and cultured for 48 hours. ⁴ / well in 96 wells and cultured for 12 hours. After culturing, Example 1 was added to each well at concentrations of 0.002%, 0.01%, 0.02%, 0.05% and 0.1%. The degree of cell activity at each concentration over 48 hours after the addition of Example 1 was measured by the MTT method and is shown in Table 3 below.

Cell viability (%) 0.002% 0.01% 0.02% 0.05% 0.1% Control group 100 Example 1 108.1 104.7 114.3 117.8 113.5

As shown in Table 3, the cell activity of Example 1 was superior to that of the control group, and it was confirmed that the cell activity was increased in proportion to the concentration particularly in the concentration range of 0.002 to 0.1%.

Example 4: Whitening effect

Melanin formation inhibition experiment

B16F10 melanoma cells (B16F10 melanoma cells) were cultured in DMEM medium supplemented with 10% fetal bovine serum at 37 ° C and 5% CO2. After the cells were cultured in the medium, the cells were washed with PBS, and the cells were removed by adding 0.05% trypsin-EDTA solution. The supernatant was removed by centrifugation at 1000 rpm for 5 minutes. The cell pellet was added to the medium, and the cells were suspended. The cell number was then measured. Cells were diluted to a concentration of 10 ⁵ cells / ml and cultured in a cell culture incubator. After confirming that the cells were completely attached, the cells were exchanged with a medium containing the compound of Example 1 or arbutin and cultured for 3 days. After obtaining the cell precipitate by the above method, the number of cells was measured, and the degree of toxicity in the cells of the sample was measured by comparing the increase and decrease of the cell number with the experimental control group to which no compound was added, and the same number of cells (10 ⁶ cells) The cell pellet was obtained and the color was visually observed.

The cell pellet was added with 1 ml of 1N NaOH, and the cells were completely dissolved by heating at 80 ° C for 1 hour, and the absorbance was measured at 475 nm with an ELISA reader. The melanin production rate of each sample was calculated in comparison with the control group not treated with the complex, and the results are shown in Table 4 below.

0.10% 0.50% One% control 100 MSH 1uM 280.4 Arbutin 0.01% 171.6 Example 1 350.4 200.2 130.0

Example 5: Exfoliating effect

Ten healthy adults (between 30 and 40 years old) were washed with washing oil to measure the L value (Lvalue) of the skin brightness before application, and the pineapple and kiwi protease and Indian gooseberry extract Controls were applied and applied three times a day, respectively. The L value (L-value), which is the degree of skin brightness, was measured at intervals of two days while applying continuously, and this experiment was performed for one week. The increase in the L value shows the extent to which the keratin is removed, and the results are shown in Table 5 below.

Before use 1 time Episode 2 Martial arts 50.22 50.15 50.19 Example 1 50.38 65.12 66.32

Example 6: Skin patch test

Twenty 20 - to - 30 - year - old women who have never been hypersensitive to skin irritation due to a history of skin irritation or skin allergy symptoms have been tested on human skin patches to confirm the skin safety of the present invention. First, the test area was wiped with 70% ethanol and then dried. 15 μg of the prepared test substance was dripped into a Finn chamber (100 × 10, EPITEST, Finland), and the test piece was hermetically sealed to the inside of the forearm. Patches were applied for 24 hours, the patches were removed, and the test area was marked with a pen. After 1 hour and 24 hours after the labeling, the test site was observed using a magnifying glass (8MC-150, DAZOR, USA) to observe erythema and edema. The skin response was determined according to the regulations of the International Contact Dermaitis Research Group (ICDRG), and the mean skin response was calculated according to Equation (2). The results are shown in Table 7.

[Evaluation Criteria and Score of Skin Response] sign score Evaluation standard - 0 No reaction ± 0.5 Faint or mild erythema + One Blurred or weak erythema, edema and palsy ++ 2 Clear erythema, papules and small follicles +++ 3 Severe erythema and alopecia, avoidant formation

Equation 2

Average Skin Response Table = (Score X Reaction Number X 100 X 1/2) / [3 (Maximum Score) X Total Number of Subjects (n)]

Test substance After 1 hour After 24 hours Response (%) (n = 20) ± + ++ ± + ++ 0.00 Martial arts - - - - - - 0.00 15% glycerin - - - - - - 0.00 Example 1 - - - - - - 0.00

Production Example 1: Softening longevity (skin) in cosmetics containing a complex containing pineapple and kiwi protease and Indian gooseberry extract

ingredient content (%) Example 1 3.0 Butylene glycol 4.5 glycerin 5.0 ethanol 10.0 Oleyl alcohol 0.1 Polysorbate 20 0.5 Propylparaben 0.1 Spices 0.3 Purified water Balance system 100

Production Example 2: Softening longevity (lotion) in cosmetics containing a complex containing pineapple and kiwi protease and Indian gooseberry extract

ingredient content (%) Example 1 3.0 Butylene glycol 4.5 glycerin 5.0 Triethanolamine 5.0 Liquid paraffin 5.0 Squalane 3.0 Macadamia nut oil 2.0 Polysorbate 60 1.0 Sorbitan sesquioleate 1.0 Carboxyvinyl polymer 1.0 Propylparaben 0.03 Spices 0.09 Purified water Balance system 100

Production Example 3: Nourishing cream among cosmetics containing a complex containing pineapple and kiwi protease and Indian gooseberry extract

ingredient content (%) Example 1 5.0 glycerin 4.0 Vaseline 3.5 Triethanolamine 0.5 Liquid paraffin 24.0 Squalane 3.0 Wax 2.1 Tocopheryl acetate 0.1 Polysorbate 60 2.4 Carboxyvinyl polymer 1.0 Sorbitan sesquioleate 2.3 Spices a very small amount antiseptic a very small amount Purified water Balance system 100

Production Example 4: Composition containing pineapple and kiwi-derived protease and Indian gooseberry extract.

ingredient content (%) Example 1 5.0 Ethyl alcohol 1.0 EDTA-2Na 0.05 Propylene glycol 5.0 Carbopol 4.5 Polyoxide 1.5 antiseptic 0.2 Spices a very small amount antiseptic a very small amount Purified water Balance system 100

Production Example 5: Body cleanser in cosmetics containing a complex containing pineapple and kiwi protease and Indian gooseberry extract

ingredient content (%) Example 1 10.0 Alkyl ether sulphate (30%) 30.0 Alkylether sulfosuccinate (30%) 20.0 Alkylamido betaine (30%) 5.0 Propylene glycol 5.0 Betaine 5.0 Laura Midi Dei 4.0 Cellulose sword 0.05 Hydrolyzed protein 0.1 Citric acid a very small amount NaCl a very small amount antiseptic a very small amount Spices a very small amount Purified water Balance system 100

Claims (5)

Pineapple, and kiwi, as an active ingredient. The cosmetic composition for wrinkle improvement, elasticity enhancement, antioxidation, whitening, exfoliation, and skin texture improvement.

Pineapple and kiwi, and indian gooseberry extract as an active ingredient. The cosmetic composition for wrinkle improvement, elasticity enhancement, antioxidation, whitening, exfoliation, and skin texture improvement is characterized by containing natural protease and Indian gooseberry extract derived from pineapple and kiwi.
The method according to any one of claims 1 to 2, wherein the protease derived from pineapple and kiwifruit is contained in an amount of 0.001 to 20.0% by weight based on the total weight of the composition. And a cosmetic composition for improving skin texture.
3. The cosmetic composition according to claim 2, wherein the extract of Indian gooseberry contains 0.01 to 50.0% by weight based on the total weight of the composition, wherein the cosmetic composition for improving wrinkles, elasticity, antioxidant, whitening, exfoliation and skin texture is improved.
The composition according to any one of claims 1 to 4, wherein the composition is selected from the group consisting of softening agents, nutritional lotions, nutritional creams, massage creams, essences, eye creams, cleansing creams, cleansing foams, cleansing waters, packs, Wherein the cosmetic composition has a formulation selected from the group consisting of: a cosmetic composition for improving wrinkles, elasticity, antioxidant, whitening, exfoliation, and skin texture.