KR101901859B1 - Cosmetic composition containing SARGASSUM FULVELLUM extract, CODIUM FRAGILE extract and UNDARIA PINNATIFIDA extract - Google Patents

Abstract

More particularly, the present invention relates to a cosmetic composition containing an extract of Momordicae extract, an auditory extract and a marine marine extract as an active ingredient, and more particularly to a cosmetic composition containing a mordant extract, an auditory extract and a marine marine extract Which is excellent in antioxidant effect and improves skin elasticity and wrinkles.

Description

[0002] Cosmetic composition containing SARGASSUM FULVELLUM extract, CODIUM FRAGILE extract and UNDARIA PINNATIFIDA extract,

More particularly, the present invention relates to a cosmetic composition containing an extract of Momordicae extract, an auditory extract and a marine marine extract as an active ingredient, and more particularly to a cosmetic composition containing a mordant extract, an auditory extract and a marine marine extract Which is excellent in antioxidant effect and improves skin elasticity and wrinkles.

The skin is largely divided into three layers of epidermis, dermis and subcutaneous adipose tissue in order from the outside. It functions to protect the human body from physical and chemical stimulation from the external environment. Especially, skin has about 65 ~ 70% of water is controlled to evaporate out of the body. Among them, the epidermis is divided into the order of stratum corneum, granular layer, superficial layer, and basal layer from the outside. The stratum corneum of the epidermis contains about 10 to 20% of water and exists in the outermost part of the human body to suppress water evaporation from the body ( J. Invest. Dermatol. 80 (Suppl.), 44-49, 1983 ). The dermis consists of the papillary layer and the reticular layer. The papillary layer is composed of connective tissues. The collagenous fibers and some elastic fibers give elasticity to the skin, which is important for skin elasticity (wrinkles). It plays a role. The subcutaneous tissue is a layer of connective tissue and sometimes also a fatty tissue layer. The subcutaneous tissue is basically composed of plastic connective tissue scattered in the subcutaneous fat layer. These adipocytes act as a buffer to preserve and protect heat and store calories.

There are two main causes of skin aging: first, as a result of aging that can not be avoided by aging due to endogenous aging; and second, as extrinsic aging typically causes long-term exposure to sunlight Speaking of it, there is photoaging and aging by active oxygen. As the stratum corneum is damaged by the ultraviolet rays in the sunlight, it begins to overproduce, and as a result, photochromism occurs as the thickness of the stratum corneum increases. In addition, damage caused by ultraviolet rays causes collagen production ability of the dermal layer to decrease, resulting in a thinner dermal layer, resulting in wrinkles that are thicker and deeper than those of endogenous aging. The fact that reactive oxygen species (ROS) is involved in skin aging is well known by many studies. The active acid novel of aging was proposed by Harman in 1956. It is known that the oxidative damage of biocomponents is caused by various active oxygen generated by secondary metabolism during normal metabolism and accumulation of these damages leads to aging and death will be. The damage caused by active oxygen is coped with the defensive ability of the living body, but the defense is not 100% complete, so it is affected by some active oxygen. This harmful effect occurs slowly and, if necessary, chronically through several years or lifetimes, accumulating to diminish the function of cells or tissues of the skin, which is the cause of disease and aging. Research on cosmetics for the purpose of improving skin elasticity and improving wrinkles has been actively carried out as consumers of skin aging have a desire to look youthful and expectations for cosmetics have increased.

In general, cosmetics are intended to clean the body, to make itself beautiful and attractive, and to protect the skin and hair from ultraviolet rays and drying to prevent aging. Recently, with the development of industrial society and the increase of social activities, self-awareness to express oneself positively has increased, and the desire to use cosmetics to keep their own skin and maintain their health is greatly increased. Accordingly, various types and formulations of foundation and color cosmetics have been researched and developed, among which skin elasticity improvement and wrinkle improvement cosmetics are recognized as the most important products in various categories.

Accordingly, a variety of cosmetic raw materials have been developed in order to improve skin wrinkles and skin elasticity, and raw materials derived from natural plants have been attracting attention rather than animal raw materials in recent years. Seaweed extracts, which retain their vitality in extreme environments in the sea and have a lot of good nutrients, are likely to be more favorable to consumers who want more powerful effects. Actual seaweed is a good source of vitamins and minerals as a food, so if you apply it to your skin, it will be a good material to deliver the effect directly to your skin.

Accordingly, the present inventors have repeatedly studied skin effect of seaweed extract. As a result, it has been found out that the extract of Momordica mellifera, the extract of audible and the sea bream extract has improved skin oxidative stress and has a wrinkle improving effect on the skin. .

Accordingly, an object of the present invention is to provide a cosmetic composition which is excellent in antioxidative effect, skin elasticity and wrinkle-reducing effect.

In order to achieve the above object, the present invention provides a cosmetic composition for skin antioxidation, skin elasticity and wrinkle as an active ingredient containing mungbean extract, auditory extract and marine marine extract.

The cosmetic composition of the present invention contains an extract of mung bean, an auditory extract and a marine marine extract to enhance the antioxidant activity of the skin, and the skin elasticity improvement effect and wrinkle reduction effect are excellent.

FIG. 1 is a graph showing the protective effect of keratinocyte extract, auditory extract, and sea bream extract of the present invention on damaging keratinocytes by ultraviolet rays.

The present invention includes at least one selected from the group consisting of mung bean extract, auditory extract and marine marine extract as an active ingredient.

Hereinafter, the present invention will be described in more detail.

The Sargassum fulvellum used as an active ingredient in the present invention has distinct roots, stems and leaves, and its roots are vastly shaped. It grows more than 1 ~ 3m with one central branch. The stem is triangular or triangular and twisted. Leaves grow from stem to base, bend, spatulate or oval, and midrib to leaf center. The leaf on the upper part is serpentine, serrated on the edge, and bubbles are formed from the stem on the whole body. It has a deep yellowish brown color and can be seen all over Korea. The main ingredients are alginic acid, chlorophyll ac, β-carotene, fucoxanthin, laminarin, and mannitol. The amount of fat is very low, but when analyzed in powder form, the amount of dietary fiber and fat is over 30%. The mackerel has a relatively high content of dietary fiber than the carbohydrate. In Korea, many kinds of grains are usually edible and used as raw materials for seaweed industry such as alginic acid, or as fertilizer. Alginic acid helps digestion of stomach, helps bowel movements, is good for constipation, and is effective in preventing colon cancer. It is good for hypertension and osteoporosis because of its high mineral content and it is good for thyroid dysfunction because of high iodine content.

In the present invention, the auditory sense ( Codium fragile ) used as an active ingredient is recorded in that the texture is smooth, the color is dark green, and the taste is good and the flavor of the kimchi is enhanced. Thus, it is used as a flavoring material of kimchi . In Japan, it is called "mil" meaning "pine" living in the sea. The height is 10 ~ 30cm, the thickness is 1.5 ~ 3mm, and the lower part is thicker. The branches are divided into the shape of a deer horn and grow straight, all reaching the same height and forming a fan shape. The surface is soft like a jungle, and microscopically, the inside of the branch is irregularly entangled like a transparent thread. The young have hair on the front side, especially on the upper side, but they fall off afterwards and leave hairless marks. The entire body is a structure in which multiple nuclei are contained in one cell, all without any intercellular membrane. The spore-containing pouch is a club-shaped, 5 to 7 times the length, and the apex is pointed. It is rich in dietary fiber and contains a large amount of minerals such as calcium, iron, vitamin A, vitamin C and niacin.

Undaria pinnatifida used in the present invention is a lobular plant with distinct roots, stems and leaves. It is distributed in the coastal area of Korea, but it is not distributed in the area where it is strongly influenced by Han River. They live in rocks near Chosun, but they tend to live deeper in the southern part and shallower in the northern part. It is mainly harvested from winter to spring and is the most delicious at this time. It breeds from spring to summer. It is a major prey of abalone and sole, and is mainly used for food in Korea, Japan, and China. Dietary fiber, potassium, calcium, and iodine are enriched to energize metabolism and have been used since early childbirth cooking, prevention of constipation, obesity, excellent in iron calcium supplement. It has already been exported to China since the Goryeo Dynasty. In Koryo Dharma, "seaweed is eaten widely without religion. It tastes delicious and fishy, but it can only be eaten if it eats for a long time.""Goryeo" means "in seoul 12 years (1058) (The seaweed seaweed picking place on the beach), "and records," Koryo 26 reign of King Chongseon (1301) in the seaweed was offered to the Yuan Dynasty ". There is a record in the Dong-bo-bo-gyeon that "the fever is cold, the taste is woven, and there is no poison. The efficacy is to remove the stiffness while the fever is healing, The dried product contains 12.7% of protein, 52% of carbohydrate, 1.1% of fat, 1300mg of Ca, 0.11mg of vitamin A, 0.14mg of B2, 10mg of nicotinic acid, 15mg of C%. Seaweed is rich in calcium and contains about 100mg of iodine and is a strong alkaline food. In addition, alginic acid is contained in the seaweed as a viscous substance, but its content in the root is smaller than that of leaves or stems. The main carotenoid pigment of the seaweed is fucoxanthine, violaxanthine, and lutein and β-carotene.

The mash moth extract, the auditory extract and the marine marine extract of the present invention can be produced according to a method common in the art. Specific examples thereof may be obtained by distillation of the lyophilized dried mackerel, the heaak and the sea bream, respectively, by steam, followed by extraction and purification using glycerin as a solvent.

The cosmetic composition of the present invention contains 0.001 to 30% by weight, respectively, based on the total weight of the composition. This is because, if it is less than 0.001% by weight, no remarkable effect can be expected. If it exceeds 30% by weight, the increase in the content does not show a marked effect.

When the cosmetic composition of the present invention is mixed with mash mash extract, auditory extract, and sea bream extract, the effect of skin antioxidation, wrinkle improvement and elasticity improvement can be further increased, and three kinds of mash extract, Containing the mixture, the effect of synergy between the active ingredients is the most effective for improving skin antioxidation, wrinkle and elasticity.

The composition for external application for skin according to the present invention contains a cosmetically and dermatologically acceptable medium and / or base. It may be in any form suitable for topical application, for example as a solution, a gel, a solid or a paste anhydrous product, an emulsion obtained by dispersing an oil phase in water, a suspension, a microemulsion, a microcapsule, a microgranule or an ionic form (liposome) and / In the form of a non-ionic follicle dispersing agent, or in the form of creams, skins, lotions, powders, ointments, sprays or conical sticks. These compositions may be prepared according to conventional methods in the art. In addition, the composition for external application for skin according to the present invention may be used in the form of a foam or in the form of an aerosol composition further containing a compressed propellant.

The composition for external application for skin according to the present invention can be used as a skin external agent composition in the form of a composition containing a lipid, an organic solvent, a solubilizer, a thickening agent and a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizer, a foaming agent, Such as cosmetics or skin, such as fillers, sequestering and chelating agents, preservatives, vitamins, barrier agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or any other ingredient commonly used in cosmetics Adjuvants conventionally used in the scientific field, and the like. These adjuvants are introduced in amounts commonly used in the cosmetic or dermatological fields.

Hereinafter, the present invention will be described in more detail with reference to examples and test examples, but the present invention is not limited to these examples.

 [Preparation Example 1] Preparation of mungbean extract

* 1 kg of lyophilized mung bean was distilled with steam for 12 hours, the distilled product was made 70% glycerin, cooled for 7 days at 4 ° C, and then purified.

[Preparation Example 2] Preparation of auditory extract

1 kg of freeze-dried hearing was distilled with steam for 12 hours, and the distilled product was 70% glycerinated, followed by cooling at 4 ° C for 7 days, followed by purification.

[Preparation Example 3] Preparation of an extract of sea bream

1 kg of freeze-dried seaweed was distilled with steam for 12 hours, and the distilled product was 70% glycerinated, followed by cooling at 4 ° C for 7 days, followed by purification.

[Preparation Example 4] Preparation of mixed extracts of mackerel, hearing and sea bream

The lyophilized moth, hearing and sea bream was mixed at a weight ratio of 1: 1: 1, and the mixture was distilled with steam for 12 hours. The distilled product was 70% glycerinated, cooled for 7 days at 4 ° C and purified to obtain .

[Preparation Example 5] Preparation of mixed extract of mother and child

The lyophilized moth and the hearing were mixed at a weight ratio of 1: 1 and distilled with steam for 12 hours. The distilled product was made into 70% glycerin, cooled for 7 days at 4 캜, and then purified.

[Preparation Example 6] Preparation of mixed extracts of mackerel and seaweed

The lyophilized moth and the sea bream was mixed at a weight ratio of 1: 1 and distilled with steam for 12 hours. The distilled product was made into 70% glycerin, cooled for 7 days at 4 ° C and purified.

[Preparation Example 7] Preparation of mixed extracts of auditory and marinated seaweed

The lyophilized hearing and sea bream was mixed at a weight ratio of 1: 1 and distilled with steam for 12 hours. The distilled product was 70% glycerinated, cooled for 7 days at 4 ° C and purified.

[Test Example 1] Protection effect of cell damage by ultraviolet rays

Keratinocytes isolated from human epidermal tissue were added to each well of a 96-well cell culture plate at 1 × 10 ⁴ cells for 24 hours. After 18 hours, the culture medium was removed, and 50 占 퐇 of phosphate buffered saline (PBS) was added to each well. The keratinocytes were irradiated with ultraviolet rays of 30 mJ / cm 2 using an ultraviolet B (UVB) lamp (Model: F15T8, UVB 15W, Sankyo Dennki, Japan), PBS was removed, keratinocyte growth media, Clonetics, BioWhittacker, MD, USA). The mash extract, the auditory extract, the sea bream extract or the mixed extract thereof of the above Preparation Examples 1 to 7 were treated and then cultured for 20 hours. After a certain period of time, an appropriate amount of the culture supernatant was taken and the amount of lactate dehydrogenase (LDH), an indicator enzyme for cell damage, was quantified for 20 hours. The amount of LDH in the culture supernatant was measured using a Cytotox 96 non-radioactive cytotoxicity assay kit from Promega (Madison, WI, USA). The LDH secretion rate (%) was obtained by calculating the relative value of each group by fixing the control vehicle as 100, and the results are shown in FIG.

As can be seen from FIG. 1, the mongolian extracts, auditory extracts, and sea bream extracts were compared with the mongolian, hearing and sea bream extracts, In the treatment of each of the extracts, the increase of LDH due to the damage of keratinocytes by UV rays could be suppressed to some extent, but the increase of LDH in the group treated with two active ingredients was significantly reduced. In particular, treatment of blended extracts of mulberry, hearing and marine swamps reduced LDH uptake to a similar degree to that of the untreated control (vehicle).

Therefore, the moth, moth, and seaweed extracts of the present invention are excellent in protecting skin cells against external stimuli such as ultraviolet rays.

[Examples 1 to 7 and Comparative Example 1]

The cream formulations of Examples 1 to 7 and Comparative Example 1 were prepared with the composition shown in Table 1 below (unit: wt%).

ingredient Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Example 7 Comparative Example 1 One Production Example 1 One 0 0 0 0 0 0 0 2 Production Example 2 0 One 0 0 0 0 0 0 3 Production Example 3 0 0 One 0 0 0 0 0 4 Production Example 4 0 0 0 One 0 0 0 0 5 Production Example 5 0 0 0 0 One 0 0 0 6 Production Example 6 0 0 0 0 0 One 0 0 7 Production Example 7 0 0 0 0 0 0 One 0 8 Purified water Balance Balance Balance Balance Balance Balance Balance Balance 9 glycerin 8 8 8 8 8 8 8 8 10 Butylene glycol 4 4 4 4 4 4 4 4 11 Hyaluronic acid extract 5 5 5 5 5 5 5 5 12 Beta Glucan 7 7 7 7 7 7 7 7 13 Carbomer 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 14 Triethanolamine Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount 15 Caprylic / capric triglyceride 8 8 8 8 8 8 8 8 16 Squalane 5 5 5 5 5 5 5 5 17 Cetearyl glucoside 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 18 Sorbitan stearate 0.4 0.4 0.4 0.4 0.4 0.4 0.4 0.4 19 Cetearyl alcohol 2 2 2 2 2 2 2 2 20 antiseptic Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount

<Production methods of Examples 1 to 7 and Comparative Example 1> 1) The above components (1) to (12) were uniformly mixed while heating to 70 ° C to prepare a water-based part.

2) The components (15) to (20) were uniformly mixed while heating to 70 ° C to prepare an oil phase part.

3) The oil phase part of 2) was put into the water phase part of the above 1) and homomixed at 7,200 rpm for 6 minutes.

4) The mixture of 3) was cooled to room temperature.

[Test Example 2] Skin elasticity enhancement effect

In order to test the effect of enhancing the elasticity in the skin of an actual person, the following tests were conducted using Examples 1 to 7 and Comparative Example 1 as follows. Twenty women aged 25 to 35 were applied to the face in the morning and evening twice a day for 8 weeks. The skin elasticity improvement effect was measured using a skin elasticity tester (Cutometer SEM 575, C + K Electronic Co., Germany) Are shown in Table 2 below. The resultant value refers to the skin viscoelasticity of the skin elasticity measuring device.

Skin elasticity improvement effect Test substance Skin elasticity effect Example 1 0.31 0.10 Example 2 0.27 ± 0.10 Example 3 0.37 + - 0.10 Example 4 0.49 + - 0.10 Example 5 0.33 + - 0.10 Example 6 0.40 0.10 Example 7 0.41 + - 0.10 Comparative Example 1 0.22 0.09

As can be seen from the results in Table 2, the group treated with the moth, the hearing and the sea bream extract alone increased skin elasticity, and the skin elasticity of the group treated with the mixture was improved. In particular, the group treated with the mixture of mulberry, hearing and marine marine extract showed the best effect of enhancing skin elasticity. Thus, the mulberry extract, audible extract, and marine marine extract of the present invention were excellent in improving skin elasticity.

[Test Example 3] Promotion of collagen production promotion

 For the comparison of collagen production, no negative control was used and vitamin C was used as a positive control. The fibroblast cell line hs68 was used to compare the productivity of type 1 procollagen and inhibition of collagenase synthesis.

Fibroblasts were plated on a FALCON 48-well plate at 5 × 10 ⁴ / well, and then subjected to treatment with 1 ppm of mulberry extract, audible extract, marigold extract or mixed extract thereof. After 24 hours of culture, the amount of type 1 procollagen produced in the culture layer was determined using a Type 1 pN collagen EIA kit. The amount of type 1 procollagen produced was corrected for total protein content and compared with the control and experimental groups. The results are shown in Table 3 below.

group Collagen production ability Vitamin C 27.8% increase Mushroom extract 15.2% increase Auditory extract 11.5% increase Seaweed extract 18.1% increase Mixed extract of moth and hearing 15.2% increase Mixed extract of mackerel and seaweed 20.4% increase Mixed extracts of hearing and seaweed 19.2% increase Moth, hearing and seaweed
Mixed extract 22.9% increase

As shown in the results of Table 3, the group treated alone with the mackerel, the hearing and the sea bream extract promoted the production of collagen, and the effect of promoting the production of collagen in the group treated with the mixture was further increased . In particular, the group treated with the mixture of mulberry, hearing and sea bream extracts showed the best effect of promoting collagen production. Therefore, the moth, moth, and seaweed extracts of the present invention promoted collagen production, and thus had an excellent effect of improving skin wrinkles.

[Test Example 4] Collagenase synthesis inhibitory effect

For the inhibition of collagenase synthesis, no substance was treated as a negative control and retinoic acid was treated as a positive control.

Fibroblasts were plated on a FALCON 48-well plate at a density of ⁵ x 10 ⁴ cells / well and irradiated with 15 mJ / cm 2 of UVB. Then, the cells were treated with mash extract, auditory extract, Was treated at a concentration of 1 ppm. After 24 hours of culture, the amount of collagenase MMP-1 (matrix metalloproteinases-1) produced in the culture supernatant was measured using an MMP-1 ELISA kit. The amount of MMP-1 was corrected to the total protein amount, and the results are summarized in Table 4 below.

group MMP-1 sum performance Retinoic acid (1M) 51.6% decrease Mushroom extract 23.1% decrease Auditory extract 19.7% decrease Seaweed extract 28.1% decrease Mixed extract of moth and hearing 23.6% decrease Mixed extract of mackerel and seaweed 33.1% decrease Mixed extracts of hearing and seaweed 30.9% decrease Moth, hearing and seaweed
Mixed extract Decreased by 46.7%

As can be seen from the results of Table 4, the group treated with the extracts of mackerel, hearing and sea bream exclusively inhibited the synthesis of collagenase, and the inhibitory effect on the synthesis of collagenase . In particular, the group treated with a mixture of mulberry, hearing and sea bream extracts showed the most inhibitory effect on the synthesis of collagenase. Accordingly, the moth, moth, and seaweed extracts of the present invention effectively inhibited the synthesis of collagenase, thereby exerting an effect of improving skin wrinkles.

Claims (4)

A cosmetic composition for inhibiting skin cell damage caused by ultraviolet rays, which comprises an extract of mackerel, hearing, and seaweed as an active ingredient,
The content of the moth, the hearing, and marine marine extract is 0.001 to 30% by weight based on the total weight of the composition,
A cosmetic composition for inhibiting skin cell damage by ultraviolet rays, characterized in that the mixed extracts of moth, hearing and sea bream ranges are prepared by mixing lyophilized moth, hearing and marinated seaweed at a weight ratio of 1: 1: 1. delete delete [3] The method according to claim 1, wherein the mixed extract of the moth, the hearing and the sea bream is prepared by mixing a lyophilized moth, an audible and a sea bream starved with steam, distilling the distilled product by 70% glycerin, Wherein the cosmetic composition is obtained by purifying the composition.

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