KR100981344B1 - The cosmetic composition for preventing the skin aging - Google Patents

Abstract

The present invention relates to a cosmetic composition for preventing skin aging containing a herbal extract extract fermentation, and more specifically, after extracting hot water from a herbal mixture consisting of sewage, baekbokyeong, dew, donkey, wolfberry, earth and sand and camphor, and using fermented microorganisms. It relates to a cosmetic composition for preventing skin aging containing a fermented product which has been fermented by fermentation.

The cosmetic composition of the present invention prevents skin aging through skin wrinkle improvement and skin elasticity improvement, moisturizing ability and skin immunity enhancement by promoting the proliferation of skin cells, anti-oxidation effect, and promotion of collagen synthesis, and through the fermentation, the toxicity of herbal medicine By removing or mitigating, damage to toxicity can be minimized. Since the active ingredient of the polymer structure is decomposed into a low molecular structure, the skin penetration effect is greatly increased, thereby maximizing the effect of the active ingredient.

Skin aging defense, sewage, baekbokyeong, hyssop, Angelica, wolfberry, earth and sand, camphor, fermentation, cosmetics, composition

Description

The cosmetic composition for preventing the skin aging}

The present invention relates to a cosmetic composition for preventing skin aging containing fermented products fermented using fermentation microorganisms after hot water extraction of a herbal mixture consisting of sewage, baekbokyeong, dew, donkey, goji berry, earth and sand, and camphor ginseng. Prevents skin aging through skin wrinkle improvement, skin elasticity improvement, moisturizing ability, and skin immunity enhancement by promotion, antioxidant effect, and collagen synthesis, and minimizes damage to toxicity by eliminating or alleviating the toxicity of herbal medicine through fermentation The present invention relates to a cosmetic composition for preventing skin aging, in which the active ingredient of the polymer structure is decomposed into a low molecular structure, thereby greatly increasing the skin penetration effect, thereby maximizing the effect of the active ingredient.

As the largest organ of the human body, which occupies about 16% of the total body volume, the skin is in direct contact with the external environment and is important to protect the body from many deadly harmful factors such as temperature, humidity, and ultraviolet radiation. Act as a shield. However, with age, skin cells are damaged by various contaminants, strong ultraviolet rays, stress and malnutrition, and cell proliferation is not properly performed, resulting in wrinkles, loss of elasticity and keratinization. .

Skin aging is largely divided into intrinsic aging and extrinsic aging (Cosmetics & Toiletories, 111: 31-37 (1996)). Endogenous aging is a aging phenomenon that occurs naturally as the physiological function of the skin gradually decreases with age. Clinically, the elasticity is reduced, the skin texture is rough, deep wrinkles are formed, and pigments are deposited. Dermatol., 130: 87-95 (1994). Exogenous aging refers to the aging phenomenon caused by exogenous factors such as ultraviolet light, reactive oxygen species and stress. These factors lead to rapid aging of the skin, resulting in rough wrinkles on the surface of the skin and a marked decrease in elasticity.

Various studies have been conducted to investigate the mechanism of aging of the skin, revealing various causes of skin aging. When the skin is dried by a change in temperature, a decrease in humidity or wind, the skin physiological activity as a protective barrier against the outside is lowered to promote aging. In addition, when the skin is exposed to harmful free radicals or free radicals, lipid peroxide is produced by oxidative action in the body and causes modification of various proteins constituting the skin. Meanwhile, collagen and elastic elastin proteins, which make up most of the dermis (about 70-80% of the total dry weight of the skin), are the major proteins produced by fibroblasts and are involved in the skin's mechanical firmness, tissue adhesion, and elasticity in the dermal layer. Journal of the American Academy of Dermatology, 21: 610-613 (1989). Collagen decreases with age and with increasing exposure to ultraviolet light. Collagen is classified according to form and structural features, the most well known of which are Type I, II, III and IV. Collagen types I and III constitute the constituents of the cytoplasm of the dermis, and collagen type IV is the major component of DEJ (Dermal Epidermal Junction). DEJ is located between the epidermal and dermal layers of the skin to regulate the permeation and transport of substances between the two layers, to control the differentiation of adjacent skin cells and to maintain the firmness of the skin. Collagen and elastin, the major structural components of the skin, are either denatured or reduced in biosynthesis by natural or external stimuli, thereby accelerating skin aging.

Research has been actively conducted to suppress or slow down the progression of skin, and cosmetics or skin protection agents containing various skin protection ingredients such as retinol, retinoid, vitamin C, flavonoids and tocopherols, and herbal ingredients have been developed. .

On the other hand, the present inventors, as a result of earnest research efforts to develop skin aging prevention, that is, skin protection cosmetic composition using folk medicine and herbal herbal medicines, herbal medicines mixed with sewage, baekbokyeong, dew, donkey, wolfberry, earthenware and camphor ginseng extract in the prior patent The extract has the effect of promoting the proliferation of the skin cells, antioxidant effect, promote collagen synthesis, enhance skin immunity and skin moisturizing effect, cosmetics containing the herbal blend extract as an active ingredient, wrinkles, reduced elasticity and loss of moisture without skin irritation It has been registered as a patent registration No. 10-0753186 by confirming that it can effectively protect the skin by improving the skin immunity by eliminating the complex skin aging phenomenon.

Accordingly, an object of the present invention is to prevent skin aging through skin wrinkle improvement and skin elasticity improvement, moisturizing and skin immunity by promoting the proliferation of skin cells, antioxidant effect, promotion of collagen synthesis, and to prevent skin aging through fermentation The removal or mitigation can minimize the damage to toxicity, and since the active ingredient of the polymer structure is decomposed into a low molecular structure, skin penetration effect is greatly increased and the skin containing the herbal mixed extract fermentation product maximizes the effect of the active ingredient. An anti-aging cosmetic composition is provided.

In order to achieve the above object as well as other objects that are easily expressed in the present invention, after extracting the herbal mixture consisting of sewage, Baekbokryeong, hyssop, Angelica, Gugija, Tosa and camphor ginseng, and then fermented with fermented microorganisms. By preparing a cosmetic composition for preventing skin aging, skin aging is prevented by improving skin wrinkles, improving skin elasticity, enhancing moisturizing power, and enhancing skin immunity by promoting skin cell proliferation, antioxidant efficacy, and promoting collagen synthesis. Toxicity is removed or mitigated to minimize the damage to the toxicity, and since the active ingredient of the polymer structure is decomposed into a low molecular structure, the skin penetration effect is greatly increased and the effect of the active ingredient can be maximized.

That is, as a result of earnest research efforts to maximize the anti-aging effect of the herbal mixed extract consisting of sewage, baekryokyeong, dew, donkey, goji berry, earthenware and camphor ginseng described in the Patent No. The fermented product fermented with fermented microorganisms after extracting the herbal mixture from the hot water, promotes better skin cell proliferation, antioxidant activity, collagen synthesis, improves skin wrinkles, improves skin elasticity, improves moisturizing ability and enhances skin immunity. In addition, by eliminating or alleviating the toxicity of herbal medicines through fermentation, the damage to toxicity can be minimized. Since the active ingredient of the polymer structure is decomposed into a low molecular structure, the skin penetration effect can be greatly increased to maximize the effect of the active ingredient. It was found that the present invention was completed.

The cosmetic composition for preventing skin aging according to the present invention prevents skin aging through skin wrinkle improvement and skin elasticity improvement, moisturizing ability and skin immunity enhancement by promoting the proliferation of skin cells, antioxidant efficacy, promotion of collagen synthesis, and through fermentation. Toxicity of the herbal medicine is removed or mitigated to minimize the damage to the toxicity, and since the active ingredient of the polymer structure is decomposed into a low molecular structure, the skin penetration effect is greatly increased, thereby maximizing the effect of the active ingredient.

The cosmetic composition for preventing skin aging according to the present invention is a herbal mixture consisting of sewage, baekbokyeong, dew, donkey, wolfberry, earth and sand, and camphor ginseng, after hot water extraction (hereinafter referred to as "chilbo yeodan"), fermentation using fermented microorganisms The fermented product (hereinafter referred to as "chilbo fermentation stage") is characterized.

Sewage used in the present invention (scientific name: Pleuropterus multiflorus ) is a perennial herb belonging to the genus Divineaceae and is known from China and is a long-growing vine medicinal plant. Tuber roots are used as tonics, tonics, tong economy, yangjeung, antitussives and used to treat colds, hemorrhage, nervous breakdowns, and arthritis.

In addition, Baek Bok-Ryeong (People name: Poria cocos ) is a medicinal mushroom that has been used as a herbal material since ancient times as a mushroom of the fungus fungi Democracy. Medicinal is the fungus that this mushroom makes on pine roots in the ground, and fruiting bodies rarely form on the surface of the fungus when it is exposed to the surface. The fruiting body of Bokryeong spreads flat on the fungal nucleus, having only 2 to 20 mm of depth and 0.5 to 2 mm of pores arranged on the surface, showing no mushroom shape, and almost white in color. Diuretics, sedatives, tonics, used for the treatment of edema, mental anxiety, palpitations, dyspepsia. In recent years, there has been an announcement that the polysaccharide pakiman has antitumor action (하나로 性 作用) as one of the ingredients, the medicinal effect is expected to be further.

The hyssop (scientific name: Achyranthes japonica ) is a root of perennial grasses of the perennial herb, used for food and medicinal purposes. Its main ingredient contains saponin and large amounts of calcium. Efficacy is under the action of blood circulation (活血 行 下) to induce women's physiology to normal, and treat lobster, postpartum abdominal pain. In addition, palliative pain (緩和 止痛) acts to treat the inflammation caused by arthritis, rheumatoid arthritis, and bruises of the knee disease, and has the action of fish (·) and diuresis. Back and legs are heavy and painful, sometimes used for muscle cramps. In addition, the blood vessels are diminished by the temporary drop in blood pressure, which can be used when you have headaches or dizziness.

Angelica (scientific name: Angelica gigas Nakai ) is the root of Angelica perennial, a perennial aromatic herb belonging to the family Apiaceae. Looking at the plant shape of the donkey 60-90㎝ high. Flowers are white and bloom at the end of main stems and branches in August-September. Fruits are long, long oval, with narrow wings at the edges. Angelica is a representative herbal medicine of blood remedies among herbal medicines to send blood and improve the clearing and blood flow. Therefore, it is greatly used for pain resulting from anemia or blood circulation disorder caused by blood. It also promotes peristalsis of the intestines and helps the body to absorb enough nutrients by releasing the gas smoothly. The components of Angelica mucin include essential oils and pyranucmarins of decursinol, decursin, nadakenetine, umbeliferon, beta-sitosterol and other water-soluble nonvolatile crystals.

Gujija (Scientific name: Lycium chinense ) is a deciduous broad-leaved shrub that grows wild around the village. In the deep soil, the main stem leans obliquely and sags to the bottom and grows well. The leaves are edible and the fruits are used for medicinal purposes. The name of each part is called Goji berries, leaves are Goji leaves, and the roots are called Jigolpi (地骨皮). Since ancient times, it is known as a mysterious medicine of the unbroken life, the fruit contains betaine, the skin is pisalin, and the leaves contain rutin. It contains a lot of vitamin A and contains a lot of minerals such as phosphorus, carbohydrates and protein. The leaves contain vitamin C, which strengthens the walls of blood vessels such as capillaries and prevents arteriosclerosis, and the fruit contains ingredients that improve blood circulation.

Cuscuta japonica Choisy is a perennial herb that grows on vines and grows in hills, fields and hills. The main stem is yellow, wire-shaped, hairless, seeds germinate, parasitic on other trees, absorbs nutrients, and grows tangled about 5m long. The leaves are like scales, 0.2cm long, triangular, and flowers are white in watery inflorescences. Small peduncles are short or short, and several are clustered together. The seeds are called tosa, and the seeds are rich in resin-like glycosides, amylases, and provitamin A. In oriental medicine, it was used as a gangjeong tonic, oil well, low back pain, and also as a sedative, analgesic and diarrhea. In the private sector, the face was washed with starch juice to remove papules. It has also been used as a sputum and blood drops.

Camphor ginseng ginseng ) is a vulgaris perennial plant belonging to the genus Asteraceae, or the root of it Panax Also called ginseng . Originally a wild plant that grows in the forests of temperate mountain areas, it is grown as demand for medicinal plants. The roots of camphor ginseng have various medicinal effects such as nourishment, tonic, kangsim, correction (補 精), health, and soothing, and have little toxicity. In recent years, many such effects have been recognized scientifically. In general, the medicinal properties of camphor ginseng, which are generally recognized in Korean medicine, are widely used as medicines such as tonic, cardiac, health, correction, soothing, and nourishment. It is also used as a drug for stomach ills, indigestion, vomiting, chest pain, laxative diarrhea, and loss of appetite.

As used in the present invention, "Chilbo Miranda" can be obtained by using various extraction methods known in the art for various herbal ingredients in the mixture, and then, for fermentation by fermentation microorganisms, hot water extraction using water is most preferred. desirable.

In the present specification, the term "fermentation" refers to a process in which microorganisms decompose organic materials using enzymes they have. Fermentation and decay reactions proceed by a similar process, but when decomposition results in a substance that is useful in our lives, it is called fermentation. Most organisms need oxygen for breathing, and oxygen breathing gives them the energy they need to work. On the other hand, creatures living in an oxygen-deficient environment, such as deep in the ground or the bottom of a lake, can adapt to the environment and get energy without oxygen. This breathing is called anoxic breathing. Fermentation is one such oxygen-free breath. When a person consumes organic matter such as carbohydrates, oxygen breaks down the organic matter into carbon dioxide and water and gains energy. However, anaerobic breathing organisms do not completely decompose organic matter, but produce other kinds of organic matter. In addition, the amount of energy generated is small. During fermentation, too, organic matter is decomposed, producing another organic matter and generating a small amount of energy. For example, put yeast in a bowl of glucose and close the lid. Yeast, a microorganism, uses enzymes to break down organic glucose. At this time, because the lid is covered, breathing through oxygen is impossible and oxygen-free breathing takes place. Yeast does not completely break down glucose, making ethanol. This fermentation is called alcohol fermentation, and alcohol fermentation can be used to make liquor such as makgeolli or beer. In addition, when lactic acid bacteria are reacted with glucose in anoxic state, lactic acid is produced. Such fermentation is called lactic acid fermentation. Yogurt, cheese, and kimchi are produced by lactic acid fermentation.

Through such fermentation, the substance is naturally decomposed by microorganisms and enzymes secreted by microorganisms, so it becomes smaller than 80 nm on average, which facilitates skin absorption. You have an advantage. In addition, toxic substances such as heavy metals, residual pesticides and the like harmful to the human body are completely decomposed by the microorganisms and are harmless to the human body.

Fermentation microorganisms used in the fermentation of the present invention is not particularly limited, and preferably include lactic acid bacteria (Lactobacillus, Streptococcus), Bacillus subtilis (Bacillus), yeast (Saccharomyces genus), fermentation by the microorganism Is effective for about 24 to 120 hours at 25 to 50 ° C, preferably for 48 to 72 hours at 30 to 40 ° C.

The cosmetic composition having an anti-aging effect according to the present invention is not particularly limited in its formulation, but may be prepared in a formulation such as a lotion, essence, cream, pack, gel, lotion, ointment, patch or spray. In the above formulation, the content of the herbal mixed extract fermentation may be contained in 0.0001 to 30% by weight, preferably 0.001 to 20% by weight based on the total weight of the composition. If the content of the herbal mixed extract fermentation is less than 0.0001% by weight relative to the total weight of the composition, it is difficult to expect the desired effect in the present invention, when it exceeds 30% by weight may cause problems in formulation stability.

The components included in the cosmetic composition of the present invention include components commonly used in cosmetic compositions, in addition to herbal mixed extract fermentation as an active ingredient, such as conventional auxiliaries such as stabilizers, solubilizers, vitamins, pigments and flavors, and carriers. do.

The cosmetic composition of the present invention has the use of skin anti-aging (skin protection). As used herein, the term "anti-aging of skin" has the meaning encompassing comprehensively the prevention and treatment of skin aging. Also, in this specification, "anti-aging of skin" and "skin protection" are used in the same sense unless otherwise specified.

The cosmetic composition of the present invention prevents skin aging through the improvement of skin wrinkles and skin elasticity, moisturizing and skin immunity by promoting the proliferation of skin cells, anti-oxidation effect, promotion of collagen synthesis, and the like. It is specified in the example. Thus, skin aging prevention (ie, skin protection), which is a use of the present invention, is construed to include wrinkle improvement, moisturizing improvement and skin elasticity improvement use. Furthermore, the herbal extract extract fermentation in the cosmetic composition of the present invention can minimize the damage to the toxicity by eliminating or mitigating the toxicity of the herb through fermentation, skin penetration effect because the active ingredient of the polymer structure is decomposed into a low molecular structure Is greatly increased to maximize the effect of the active ingredient.

The following examples further illustrate the invention, but are not intended to limit the scope of the invention.

Production Example  One: " Chilbo Beauty Group Manufacture of Herbal Blend Extracts

First, sewage, baekbokyeong, hyssop, donkey, wolfberry, earthenware and camphor ginseng are completely dried, and then mixed with dry weight of 40g, baekbokyeong 40g, 10g, donkey 10g, wolfberry 10g, earthworm 10g, and camphor ginseng 5g, respectively. 10-fold volume of water was added as a solvent. It was extracted by heating at 70 ° C. for about 1 hour, filtered and cooled to room temperature, then aged at low temperature at 4 ° C. for 7 days and filtered.

Production Example  2: " Chilbo Beauty Fermentation Team Herbal Blend Extract Fermented  Produce

200 ml of the "Chilbo Miranda" herbal mixture extract prepared in Preparation Example 1 was placed in a culture vessel and Bacillus subtilis ) was inoculated at 0.01% by weight with respect to the herbal mixture extract, fermented for 72 hours in a shaking incubator at 35 ° C. and used to remove fermentation microorganisms from the “chilbo-fermented soybean” herbal mixture extract fermentation through microfiltration. It was.

Experimental Example  1: skin cell proliferation effect

To examine the effect of "chilbo beauty fermentation group" on skin cell proliferation, human normal fibroblasts (Korea Cell Line Bank) were inoculated to 1 x 10 ⁴ cells in each well of a 96-well microplate. And incubated at 37 ℃ for 24 hours in DMEM medium (Sigma). Subsequently, the experimental group in which the sample was replaced with serum-free DMEM medium containing the final concentrations of 0.01%, 0.05%, 0.1%, 0.5%, and 1% and the control group in which the sample was replaced with serum-free DMEM medium containing no sample were 24 Further incubation for hours. Then, 10 μl each of MTT solution (3- (4,5-dimethylthiazol-2-yl) 2,5-diphenyltetrazolium bromide: 5 mg / ml) was added thereto, followed by incubation for 4 hours. The medium was removed, shaken for 20 minutes by addition of 100 μl of DMSO solution per well, and the absorbance of the supernatant was measured at 570 nm using a microplate reader (Molecular Devices, USA). The measured value was substituted into the following formula to calculate the cell proliferation effect, and the results are shown in Table 1 below.

Cell proliferation effect (%) = [(absorbance of experimental group-absorbance of control group) / absorbance of control group] x 100

density(%) Chilbo Beauty Group Chilbo Beauty Fermentation Team Absorbance (570nm) Cell proliferation effect (%) Absorbance (570nm) Cell proliferation effect (%) Control 0.631 - 0.620 - 0.01 0.765 21.2 0.821 32.4 0.05 0.854 35.3 0.954 53.9 0.10 0.981 55.5 1.214 95.8 0.50 1.064 68.6 1.272 105.2 1.00 1.245 97.3 1.368 120.6

As can be seen in Table 1, when the chilbo beauty fermentation stage is treated, it shows a higher fibroblast proliferation effect than the chilbo beauty end.

Experimental Example  2: antioxidant effect

A free radical scavenging activity test was conducted to investigate the antioxidant effect of the chilbo beauty fermentation step obtained according to the preparation example of the present invention. The free radical scavenging activity was modified from the method of Kim et al . ( Kor . J. Pharmacogn ., 24 (4), 299-303 (1993)), and the stable free radical DPPH (1,1-diphenyl-2- picryhydrazyl (Sigma) reagent was used. 150 μl of a 0.2mM DPPH solution (ethanol in the case of Blanc) was prepared at various concentrations, and 150 μl of these samples were added and mixed, and allowed to stand at room temperature for 30 minutes, and then an experimental group was measured for absorbance at 517 nm. As a control for this, it was set as the case using purified water. After measuring the absorbance of the experimental group and the control group, the free radical scavenging activity was obtained using the following equation, and the results are shown in Table 2 below.

Free radical scavenging effect (%) = 100-[(experimental absorbance-Blank absorbance) / control absorbance] x 100

density(%) Free radical scavenging effect (%) Cloisonne Beauty Group Chilbo Beauty Fermentation Team 0.01 20.2 32.3 0.05 38.1 49.6 0.10 57.9 62.3 0.50 79.2 95.2 1.00 93.7 95.7

As can be seen from Table 2, it was confirmed that the chilbo beauty fermentation herb herbal extract extract fermentation of the present invention is very excellent free radical scavenging ability.

Experimental Example  3: collagen synthesis promoting effect

Human normal fibroblasts (Korea Cell Line Bank) were seeded to 2 x 10 ⁴ cells in each well of a 96-well microplate and incubated for 24 hours at 37 ° C in DMEM medium. Subsequently, the experimental group in which the sample of the prepared Chilbo beauty fermentation step was replaced with the serum-free DMEM medium containing the final concentration of 0.01, 0.05 and 0.10%, respectively, and the control group replaced with the serum-free DMEM medium containing no sample 48 Further incubation for hours. After incubation, the supernatant of each well was collected and the amount of newly synthesized collagen was measured by measuring the amount of procollagen type I C-peptide (PICP) using a kit (Takara, Japan). PICP amount was converted to ng / 2 × 10 ⁴ cells, and the results are shown in Table 3 below.

 density(%) Collagen Synthesis (ng / 2 x 10 ⁴ ) Chilbo Beauty Group Chilbo Beauty Fermentation Team Control 124 127 0.005 159 175 0.01 187 201 0.05 223 249

As can be seen in Table 3, it can be seen that the synthesis of collagen increases as the concentration of the herbal mixture extract fermentation of the present invention increases, and showed a higher collagen synthesis enhancement effect than the herbal mixture extract.

Experimental Example  4: Skin immune enhancing efficacy test

After shaving the abdomen in 8-10 week-old C57BL / 6 mice, irradiation with ultraviolet B wavelength (UVB) of 2.4KJ / m ² using FS-20 sun lamp resulted in contact hypersensitivity. This results in immunosuppression, which is 44.3% inhibited compared to the control.

Samples of the chilbo beauty fermentation step of the present invention was applied after UV irradiation and after 3 days, sensitized with 3% trinitrochlorobenzene in the UV irradiation area. After 6 days, 1% trinitrogen chloride was applied to the ears of the mice, the edema state was measured, and the results are shown in Table 4.

 density(%) Skin immune enhancement effect (%) Cloisonne Beauty Group Chilbo Beauty Fermentation Team Control - - 0.01 12.6 21.5 0.05 24.5 35.6 0.10 39.5 51.2

As can be seen in Table 4, it can be seen that the herbal mixed extract fermentation product of the present invention increases the skin immune response in a concentration-dependent manner.

Example  And Comparative example

To prepare a cosmetic composition having a composition ratio of Table 5.

ingredient Example Comparative Example 1 Comparative Example 2 Content (% by weight) Content (% by weight) Content (% by weight) Cloisonne Mirror (Manufacturing Example 1) - 2.0 - Chilbo beauty fermentation stage (manufacturing example 2) 20 - - A Cetostearyl alcohol 2.0 2.0 2.0 Glyceryl Stearate 1.5 1.5 1.5 Mikey-Crystalline 0.7 0.7 0.7 Squalane 5.0 5.0 5.0 Liquid paraffin 3.0 3.0 3.0 Trioctanoine 5.0 5.0 5.0 Polysorbate 1.2 1.2 1.2 Sorbitan stearate 0.5 0.5 0.5 Tocopherol Acetate 0.2 0.2 0.2 Micromethicone 3.0 3.0 3.0 BHT 0.05 0.05 0.05 B glycerin 4.0 4.0 4.0 1,3-butylene glycol 2.0 2.0 2.0 EDTA-2Na 0.05 0.05 0.05 Purified water To 100 To 100 To 100 Incense, preservative Quantity Quantity Quantity

Experimental Example  5: moisturizing effect

In order to evaluate the moisturizing power of each of the cosmetic preparations prepared in Examples and Comparative Examples, the moisture retention ability and the water evaporation amount of the skin to which each cosmetic was applied were measured as follows.

moisture Capacity  Measure

In the constant temperature and humidity room of 22 ° C. and 45% RH, each cosmetic prepared in Examples and Comparative Examples was coated with a predetermined amount (0.03 g / 16 cm 2) in the lower half of 30 test subjects, before application, after 1 hour of application, and The moisture content of the skin 2 hours after application was measured, and normal skin without any treatment was used as a control. The measuring device used a moisture content measuring instrument (corneometer CM825, Conrage + Khazaka, Germany) to measure the moisturizing power by measuring the electric capacity of the skin according to the moisture content of the skin. The measurement results are shown in Table 6 below.

division Example Comparative Example 1 Comparative Example 2 Control Skin electrical conductivity before application 50 50 50 50 Skin electrical conductivity 1 hour after application 89 83 69 50 Skin electrical conductivity 2 hours after application 81 73 57 50

Measurement of water evaporation amount

Tape stripping on the upper arm of the arm weakens the skin's protective film, and then applies each cosmetic to the subject in the same manner as the method of measuring water retention, and then a water evaporator (Tewameter TM300, Conrage + Khazaka) , Germany Federal Bureau) to measure the water evaporation 5 times at 1 minute intervals every 12 hours to obtain an average value, the results are shown in Table 7 below.

division Example (%) Comparative Example 1 (%) Comparative Example 2 (%) Control group (%) Moisture evaporation after 0 hours 100.0 100.0 100.0 100.0 Moisture evaporation after 12 hours 76.3 81.3 89.5 93.0 Moisture evaporation after 24 hours 56.5 63.5 76.6 85.3 Moisture evaporation after 36 hours 41.9 48.0 65.2 79.5 Moisture evaporation after 48 hours 24.7 31.5 53.0 72.6

As can be seen from the results of the above experimental example, it can be seen that the cosmetics of the examples of the present invention have a higher electrical conductivity of the skin and a smaller amount of moisture loss than the cosmetics of the comparative example, and thus exhibit a better moisturizing effect.

Experimental Example  6: skin elasticity effect

The skin elasticity enhancing effect of the cosmetic composition containing the chilbo beauty fermented herb herbal extract extract fermentation of the present invention was measured.

Thirty healthy women (mean age 37 years old) of 20 years or older (mean age 37 years) were divided into three groups under the conditions of the temperature of 24-26 degreeC, and humidity 75, Example A for group A, comparative example 1 for group B, and comparative example for group C Formulation 2 was applied twice a day for 12 weeks (morning and evening) around the eyes, and skin elasticity was measured using a skin elasticity measuring instrument (Cutometer MPA580, Conrage + Khazaka, Germany). The test results were described as R5 (R5 (12 weeks) -R5 (0 weeks)) value of cutometer MPA580, where R5 value represents the nature of skin elasticity (net elasticity), and the results are shown in Table 8 below.

Experimental Formulation Skin elasticity effect Example 0.12 Comparative Example 1 0.08 Comparative Example 2 0.02

As shown in the results of Table 8, it was confirmed that the skin elasticity of the example containing the herbal mixed extract fermentation was increased compared to Comparative Example 1 containing 2% herbal extract extract and Comparative Example 2 containing nothing. .

Based on the results of the above experimental example, a cosmetic composition containing the herbal mixed extract fermentation product of the present invention is prepared and presented. However, the composition of the present invention is not limited to the following formulation examples.

Formulation example  1: Softener (skin lotion)

To the flexible composition according to the composition shown in Table 9 was prepared according to a conventional method.

Compounding ingredient Content (% by weight) Production Example 2 1.0 1.3-butylene glycol 6.0 glycerin 4.0 Oleyl alcohol 0.1 Polysorbate 20 0.5 ethanol 15.0 Benzophenone-9 0.05 Incense, preservative a very small amount Purified water to 100

Formulation example  2: nutrition cosmetics Milk Croissant )

Nutrients according to the composition shown in Table 10 was prepared according to a conventional method.

Compounding ingredient Content (% by weight) Production Example 2 3.0 Propylene glycol 6.0 glycerin 4.0 Triethanolamine 1.2 Tocophenyl Acetate 3.0 Liquid paraffin 5.0 Squalane 3.0 Macadamia Nut Oil 2.0 Polysorbate 60 1.5 Sorbitan sesquioleate 1.0 Carboxyvinyl Polymer 1.0 Bietchiti 0.01 EDTA-2Na 0.01 Incense, preservative a very small amount Purified water to 100

Formulation example  3: nutrition cream

Nutritional creams were prepared according to the conventional method in the composition shown in Table 11.

Compounding ingredient Content (% by weight) Production Example 2 10.0 Cetostearyl alcohol 2.0 Glyceryl Stearate 1.5 Trioctanoine 5.0 Polysorbate 60 1.2 Sorbitan stearate 0.5 Squalane 5.0 Floating paraffin 3.0 Cyclomethicone 3.0 BH 0.05 Delta-tocopherol 0.2 Concentrated glycerin 4.0 1,3-butylene glycol 2.0 Santa sword 0.1 EDTA-2Na 0.05 Incense, preservative a very small amount Purified water to 100

Formulation example  4: massage cream

Massage cream was prepared according to the conventional method in the composition shown in Table 12.

Compounding ingredient Content (% by weight) Production Example 2 1.0 Propylene glycol 6.0 glycerin 4.0 Triethanolamine 0.5 Beeswax 2.0 Tocopheryl acetate 0.1 Polysorbate 60 3.0 Sorbitan sesquioleate 2.5 Cetearyl Alcohol 2.0 Liquid paraffin 30.0 Carboxy Vinyl Polymer 0.5 Incense, preservative a very small amount Purified water to 100

Formulation example  5: pack

The pack was prepared according to a conventional method with the composition shown in Table 13 below.

Compounding ingredient Content (% by weight) Production Example 2 2.0 Propylene glycol 2.0 glycerin 4.0 Carboxyvinyl Polymer 0.3 ethanol 7.0 Fiji-40 Hydrogenated Castor Oil 0.8 Triethanolamine 0.3 Bietchiti 0.01 EDTA-2Na 0.01 Incense, preservative a very small amount Purified water to 100

Claims (6)

At least one selected from the group consisting of lactic acid bacteria of the genus Lactobacillus, Streptococcus, Bacillus subtilis, and Saccharomyces sp. Skin inoculation prevention cosmetic composition comprising a fermented product inoculated with the above fermentation at 25 ~ 50 ℃ for 24 to 120 hours. The cosmetic composition for preventing skin aging according to claim 1, wherein the herbal extract extract fermentation is contained in an amount of 0.0001 to 30% by weight based on the total weight of the composition. delete delete The cosmetic composition for preventing skin aging according to claim 1, wherein the composition exhibits skin wrinkle improvement and skin elasticity improvement and a combination thereof by promoting skin cell proliferation, antioxidant efficacy, and collagen synthesis. The composition of claim 1, wherein the composition has a formulation selected from the group consisting of flexible lotion, nutrition lotion, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray and powder. A cosmetic composition for preventing skin aging.