KR102400090B1 - Cosmetic Composition for Reducing Skin Wrinkle - Google Patents

Abstract

The present invention relates to a composition for external application for skin and a cosmetic composition for promoting collagen biosynthesis, comprising a complex essential oil as an active ingredient, wherein the composition can further alleviate skin wrinkles by promoting collagen synthesis, and maintain skin firmer and elastic effect can be obtained.

Description

Cosmetic composition for wrinkle improvement {Cosmetic Composition for Reducing Skin Wrinkle}

The present invention relates to a composition for external application for skin and a cosmetic composition for promoting collagen production, comprising a complex essential oil of cypress essential oil, lavender essential oil, rosemary essential oil, geranium essential oil, and bay oil.

The skin is the largest tissue in the integumentary system consisting of multiple epithelial tissues that protect the muscles and organs in the body, and plays an important role in maintaining homeostasis in the body by protecting the inside of the body from the external environment. As a result, the normal skin function of the skin is lowered due to external physical and chemical stimulation, stress, nutritional deficiency, etc., and skin aging phenomena such as reduced elasticity and wrinkle formation are accelerated.

Skin aging can be divided into innate age and extrinsic age. The intrinsic aging is aging that occurs with increasing age, and is aging that occurs due to a decrease in physiological functions of the body. In addition, the extrinsic aging refers to an aging phenomenon caused by external ultraviolet rays, active oxygen, stress, and the like. The aging of the skin proceeds due to these intrinsic and extrinsic aging factors, and as a result, wrinkles are generated on the skin surface, resulting in rough skin texture and reduced elasticity.

According to the mechanism of wrinkle formation, decomposition and production of collagen and elastin in the dermis continue to occur. is created

Representative anti-wrinkle and anti-aging substances reported so far include retinoic acid, retinol, ascorbic acid, and α-hydroxy acid.

Retinoic acid inhibits lipid per-oxidation and ornithine decarboxylase response. However, there is a problem in that it has a sensitive reaction to ultraviolet rays and has a side effect of reddening the skin due to high irritation, and its use is limited.

Although retinol has a weaker wrinkle improvement effect than retinoic acid, it is widely used because of its relatively high safety. However, when retinol is used in high concentration, skin irritation occurs, skin pigmentation occurs when exposed to ultraviolet rays, and it is easily oxidized by oxygen, heat and light in the air, so there is a problem in stability.

Ascorbic acid is easily oxidized by external environments such as air, moisture, and high temperature, and thus its activity is greatly reduced, so the degree of wrinkle improvement is lowered, and there is a problem of causing discoloration and discoloration of the formulation.

In addition, α-hydroxy acid is also disclosed as an anti-wrinkle agent, but there is a problem in the safety aspect of the product because it is accompanied by side effects of skin irritation such as itchiness, erythema, and sensitivity to ultraviolet rays.

In addition, cosmetics containing sunscreens, moisturizers, antioxidants, etc. are widely used to prevent wrinkles, but the actual wrinkle improvement effect of these products is insignificant.

On the other hand, essential oil is extracted from the fruits, leaves, flowers, stems, bark and roots of existing trees, and is named after the extracted plant. Essential oil extracted from plants is not a single chemical component, but a mixture of monoerpene-based and phenolics-based compounds with strong volatile components, and the composition of essential oil components varies depending on the genetic characteristics of the extracted plant species, the environment of the cultivation area, and the collection period. These essential oils do not directly affect the growth and differentiation of plants, but are substances that exhibit various functions, and are widely used in medicines, fragrances, and daily necessities.

As an example, Korean Patent Laid-Open No. 2014-0062958 discloses an aroma oil composition and cosmetic composition having an effect of reducing and preventing body fat or cellulite containing an essential oil fraction extracted from a sesin extract, and a cosmetic composition for preventing and improving depression. is starting

Korean Patent Publication No. 2014-0062958

The present inventors identified the effects of cypress essential oil, lavender essential oil, rosemary essential oil, geranium essential oil and bay oil used as raw materials for various aromatherapy and cosmetic products when applied to the skin. The present invention was completed by confirming that when used together rather than when used together, collagen production was stimulated, thereby showing an effect of improving skin wrinkles.

Accordingly, an object of the present invention is to provide a composition for external application for skin for accelerating collagen synthesis comprising, as an active ingredient, a complex essential oil of cypress essential oil, lavender essential oil, rosemary essential oil, geranium essential oil, and bay oil.

Another object of the present invention is to provide a cosmetic composition for wrinkle improvement comprising a complex essential oil of cypress essential oil, lavender essential oil, rosemary essential oil, geranium essential oil and bay oil as an active ingredient.

In order to achieve the above object, the present invention

Provided is a composition for external application for skin for promoting collagen biosynthesis, which contains a complex essential oil of cypress essential oil, lavender essential oil, rosemary essential oil, geranium essential oil, and bay oil as an active ingredient.

Also, the present invention

Provided is a wrinkle-improving cosmetic composition comprising a complex essential oil of cypress essential oil, lavender essential oil, rosemary essential oil, geranium essential oil and bay oil as an active ingredient.

The composition for external application and the cosmetic composition according to the present invention act on fibroblasts of the dermal layer of the skin to promote collagen production, and thus can be usefully used to prevent or improve skin wrinkles, reduced skin elasticity, or aging.

1 is a graph showing the cytotoxicity results of cypress essential oil against human skin fibroblasts.
2 is a graph showing the collagen production increasing activity in dermal fibroblasts of cypress essential oil (blue bar: after 24 hours, red bar: after 48 hours).
3 is a graph showing the cytotoxicity of lavender essential oil to human skin fibroblasts.
4 is a graph showing the collagen production increasing activity in dermal fibroblasts of lavender essential oil.
5 is a graph showing the cytotoxicity results of rosemary essential oil to human skin fibroblasts.
6 is a graph showing the collagen production increasing activity in dermal fibroblasts of rosemary essential oil.
7 is a graph showing the cytotoxicity results of geranium essential oil to human skin fibroblasts.
8 is a graph showing the collagen production increasing activity in dermal fibroblasts of geranium essential oil.
9 is a graph showing the cytotoxicity results of bayyu to human skin fibroblasts.
10 is a graph showing the collagen production increasing activity in dermal fibroblasts of Bayou.
11 is a graph showing the activity of increasing collagen production in dermal fibroblasts of cypress essential oil, lavender essential oil, rosemary essential oil, bay oil, geranium essential oil and complex essential oil (blue bar: after 24 hours, red bar: after 48 hours) .
12 is a graph showing the activity of increasing collagen production in dermal fibroblasts after culturing for 48 hours by treating each of cypress essential oil, lavender essential oil, rosemary essential oil, bay oil, geranium essential oil and complex essential oil.

Cypress essential oil, lavender essential oil, rosemary essential oil, bay oil, and geranium essential oil used as active ingredients in the present invention are separable by conventional methods in the art, that is, conventional extraction, distillation or compression, or commercially available. , the extraction method of essential oil is omitted in the present invention.

The cypress essential oil is extracted from the flowers, leaves, stems, and roots of the cypress tree (Chamaecyparis obtusa) and is known for its aroma, insecticidal, antibacterial, and antioxidant effects.

The lavender essential oil is lavender angustifolia ), which is extracted from flowers and leaves, and has been used for antibacterial, soothing, antidepressant and skin wounds.

The rosemary essential oil is rosemary ( Rosmarinus officinalis ) Extracted from the whole above-ground part of the plant, it is a natural fragrance with good antiseptic, antibacterial and astringent effects.

The geranium essential oil is geranium ( Pelargonium Graveolens ), which is extracted from flowers, is known to relieve neuralgia pain when applied topically, and has been mainly used as a flavoring and fragrance.

The bay oil is Pimenta racemosa ( Pimenta racemosa ) leaf, and has been used to relieve bronchitis and relax muscles due to its antibacterial and anti-inflammatory properties.

According to one embodiment of the present invention, the complex essential oil of cypress essential oil, lavender essential oil, rosemary essential oil, bay oil and geranium essential oil is cypress essential oil: lavender essential oil: rosemary essential oil: geranium essential oil: 500-1500: bay oil essential oil: 8-12 : 450~550 : 0.5~1.5 : Use a mixed product in a weight ratio of 450~550. This content ratio is in a range to secure the effect to be obtained in the present invention for alleviating skin wrinkles and improving skin firmer and more elastic, and it is preferable to maintain the above range.

In addition, the composition according to the present invention may contain other components capable of giving a synergistic effect to the main effect, preferably within a range that does not impair the main effect of the present invention, in addition to the complex essential oil.

As a result of an experiment to investigate the collagen synthesis effect of the complex essential oil through Experimental Example 11 according to the present invention, the amount of collagen synthesis in the skin dermal fibroblasts was further increased when the complex essential oil was treated than when each essential oil was used alone.

Through these results, the complex essential oil presented in the present invention can be applied as a composition for external application to the skin and a cosmetic composition. You can get the effect of keeping it.

The complex essential oil may include 0.0001 to 30.0 wt%, preferably 0.001 to 30.0 wt%, based on the total weight of the composition for external application. If the content of the compound is less than the suggested range, a clear effect cannot be expected, and on the contrary, if it exceeds the suggested range, the increase in the effect is not reached compared to the increase in the content and may cause some cytotoxicity.

The composition for external application is not particularly limited in its formulation, and may be, for example, an external composition having a formulation of body lotion, ointment, gel, cream, patch or spray. In this case, in the composition for external application of each dosage form, other components other than the above-described complex essential oil may be appropriately selected and formulated by those skilled in the art according to the dosage form or purpose of use of other external preparations.

In particular, the complex essential oil according to the present invention can be applied as a cosmetic composition containing it as an active ingredient through the effect of promoting collagen synthesis.

The complex essential oil may be included in an amount of 0.0001 to 30.0% by weight, preferably 0.001 to 30.0% by weight, based on the total weight of the cosmetic composition. If the content of the compound is less than the suggested range, a clear effect cannot be expected, and on the contrary, if it exceeds the above range, the increase in the effect is not reached compared to the increase in the content and may exhibit some cytotoxicity.

The composition of the cosmetic composition is not particularly limited in its formulation, for example, lotion, emulsion, cream, foam, essence, serum, soft water, emulsion, foundation, makeup base, soap, sunscreen cream and sun oil. It can be prepared in a dosage form.

In addition, the composition of each formulation may contain various bases and additives necessary and appropriate for the formulation of the formulation, and within the range that does not reduce the effect, nonionic surfactants, silicone polymers, extenders, fragrances, preservatives, Fungicides, oxidative stabilizers, organic solvents, ionic or nonionic thickeners, emollients, antioxidants, free radical scavengers, opacifiers, stabilizers, emollients, silicones, α-hydroxy acids, defoamers, humectants, It is prepared with known compounds such as vitamins, insect repellents, fragrances, preservatives, surfactants, anti-inflammatory agents, substance P antagonists, fillers, polymers, propellants, basifying or acidifying agents, or coloring agents.

Hereinafter, preferred examples are presented to help the understanding of the present invention. However, the following examples are only provided for easier understanding of the present invention, and the content of the present invention is not limited by the examples.

Experimental example 1: Confirmation of cytotoxicity of cypress essential oil

1. Culture of Human Skin Fibroblasts

Human dermal fibroblasts were purchased from Gibco (Life Technologies, Carlsbad, CA, USA). Cells were cultured at 37°C in DMEM medium (Gibco). The medium was maintained until the adherent cells were 80% confluent, and subculture was performed at 80% confluence. Medium was changed every 2 days until confluence. For the subculture, the flask from which the culture medium was removed was washed with phosphate buffer saline (PBS), the cells were dropped with 0.25% trypsin-EDTA (Gibco BRL, Grand Island, NY, USA), and the cell suspension was centrifuged. Then, the number of cells was measured, and subcultured in the culture medium three times again.

2. Measurement of Cytotoxicity

DMEM medium (Gibco) with 10% serum added to each 96-well plate was put, and human skin fibroblasts 5 X 10 ² were inoculated and cultured at 5% CO ₂ and 37° C. to attach the cells to the culture dish. Then, after culturing in a culture medium from which serum was removed for 24 hours, they were divided into a negative control group (DMSO treatment) and a group treated with cypress essential oil. 10 μl of cypress essential oil was dissolved in DMSO for each concentration (treatment concentration: 0.0001 wt% to 0.1 wt%).

After the treatment, incubated for 24 hours at 5% CO ₂ and 37° C. conditions. After adding MTT reagent, the medium was removed 4 hours later, 200 μl of DMSO was added to dissolve the formazan crystal, and the absorbance was measured at 570 nm with a microplate reader. The results are shown in FIG. 1 .

From the results of FIG. 1, it can be seen that, based on the control group, there is no cytotoxicity at the final concentration of 0.1 wt% or less under the conditions treated with cypress essential oil, and the cell proliferation is increased by about 2-7% at some of the total concentrations. there was.

Experimental example 2: Effect of increasing collagen synthesis in dermal fibroblasts by cypress essential oil

In order to confirm the effect of cypress essential oil on the amount of collagen synthesis in dermal fibroblasts, a collagen measurement experiment was performed. The dermal fibroblasts were added to DMEM medium supplemented with 10% fetal bovine serum, 100 U/ml penicillin, and 100 μg/ml streptomycin, and cultured at 37° C., 5% CO ₂ , conditions. After culturing for one day, the medium was changed to 0.01 and 0.001 wt% cypress and cultured for 24 hours and 48 hours, respectively. The wells were washed 3 times with PBS, and 100 μl of an antibody-POD conjugate solution was added to the wells and left at 37° C. for 1 hour. The wells were washed 3 times with PBS, and 100 μl of a substrate solution (TMB) was added to the wells and left at 25° C. for 30 minutes. 100 μl of 1N H ₂ SO ₄ was put into the well and absorbance was measured at 450 nm using an ELISA reader (Bio-Tek instrument) for the amount of collagen. At this time, the results were derived by calculating the collagen production increasing activity according to the relative difference in absorbance with the DMSO-treated control group. The results are shown in FIG. 2 .

Referring to FIG. 2 , when the degree of collagen synthesis was measured, when the cypress essential oil was treated for 24 hours, the collagen synthesis amount was about 120% at the 0.01 wt% concentration, and it was confirmed that the collagen synthesis amount was increased at other concentrations. However, when cypress essential oil was treated for 48 hours, the amount of collagen synthesis was rather decreased. It was found that the collagen synthesis promoting effect of cypress essential oil appeared and disappeared very quickly.

Experimental example 3: Confirmation of cytotoxicity of lavender essential oil

The cytotoxicity of lavender essential oil to human skin fibroblasts was confirmed in the same manner as in Experimental Example 1, and the results are shown in FIG. 3 .

From the results of Figure 3, it can be seen that, based on the control group, there is no cytotoxicity at the final concentration of 0.05% or less under the conditions treated with the lavender essential oil of the present invention, and the cell proliferation increases by about 20% at some very low concentrations. can

Experimental example 4: Effect of increasing collagen synthesis in dermal fibroblasts by lavender essential oil

The effect of increasing collagen synthesis in dermal fibroblasts of lavender essential oil was confirmed in the same manner as in Experimental Example 2, and the results are shown in FIG. 4 .

Referring to FIG. 4 , when 0.0001 wt% of lavender essential oil was treated for 24 hours, the amount of collagen synthesis was shown to be about 126%, and when treated for 48 hours, the amount of collagen synthesis decreased to about 91%. It was found that the collagen synthesis promoting effect of lavender essential oil appeared and disappeared very quickly.

Experimental example 5: Rosemary Confirmation of Cytotoxicity of Essential Oils

The cytotoxicity of rosemary essential oil to human skin fibroblasts was confirmed in the same manner as in Experimental Example 1, and the results are shown in FIG. 5 .

As shown in FIG. 5 , it was found that there was no cytotoxicity at the final concentration of 0.1% or less in the condition treated with rosemary essential oil, and the cell proliferation increased by about 10% at a portion of the total concentration in the condition treated with rosemary essential oil as a reference.

Experimental example 6: Rosemary Effect of increasing collagen synthesis in dermal fibroblasts by essential oil

The effect of increasing collagen synthesis in dermal fibroblasts of rosemary essential oil was confirmed in the same manner as in Experimental Example 2, and the results are shown in FIG. 6 .

Referring to FIG. 6 , when 0.01 wt% of rosemary essential oil and 0.005 wt% were treated for 24 hours, the collagen synthesis amount was about 130% at a concentration of 0.005 wt%. However, when rosemary essential oil was treated for 48 hours, the amount of collagen synthesis was rather decreased. It was found that the collagen synthesis promoting effect of rosemary essential oil appeared and disappeared very quickly.

Experimental example 7: Confirmation of cytotoxicity of geranium essential oil

The cytotoxicity of geranium essential oil to human skin fibroblasts was confirmed in the same manner as in Experimental Example 1, and the results are shown in FIG. 7 .

From the results of FIG. 7 , it can be seen that there is no cytotoxicity at a final concentration of 0.01 wt% or less in the condition treated with geranium essential oil when compared to the control group, and cell proliferation is increased by about 135% at a very low concentration.

Experimental example 8. Effect of increasing collagen synthesis in dermal fibroblasts by geranium essential oil

The effect of increasing collagen synthesis in dermal fibroblasts of rosemary essential oil was confirmed in the same manner as in Experimental Example 2, and the results are shown in FIG. 8 .

As shown in FIG. 8 , when 0.00001 wt% of geranium essential oil was treated for 24 hours, about 130% of collagen synthesis was shown, and when treated for 48 hours, there was no change in the amount of collagen synthesis. It was found that the collagen synthesis promoting effect of geranium essential oil appeared and disappeared very quickly.

Experimental example 9: bayou's Cytotoxicity check

The cytotoxicity of Bayou to human skin fibroblasts was confirmed in the same manner as in Experimental Example 1, and the results are shown in FIG. 9 .

From the results of FIG. 9 , it was found that there was no cytotoxicity at the final concentration of 0.005 wt% or less under the conditions of treatment with bayyu, and the cell proliferation increased by about 10% at a very low concentration, based on the control group.

Experimental example 10: to the bay Effect of increasing collagen synthesis in dermal fibroblasts by

The effect of increasing the collagen synthesis in dermal fibroblasts of bayyu was confirmed in the same manner as in Experimental Example 2, and the results are shown in FIG. 10 .

Referring to FIG. 10 , when the degree of collagen synthesis was measured, when 0.0005 wt% of bay oil was treated for 24 hours, about 104% collagen synthesis was shown, and the degree of increase was weak, whereas the amount of collagen synthesis when treated for 48 hours increased by about 140%. This indicates that the collagen synthesis effect is increased when bayyu is continuously used.

Experimental example 11: Effect of complex essential oil to increase collagen synthesis in dermal fibroblasts

In order to confirm the effect of the four essential oils and bay milk on the amount of collagen synthesis in dermal fibroblasts, a collagen measurement experiment was conducted using a sample mixed with five essential oils. The dermal fibroblasts were added to DMEM medium supplemented with 10% fetal bovine serum, 100 U/ml penicillin, and 100 μg/ml streptomycin, and cultured at 37° C., 5% CO ₂ , conditions. After culturing for one day, the medium was changed to a medium containing 5 types of essential oils (cypress essential oil 0.01 wt%, lavender essential oil 0.0001 wt%, rosemary 0.005 wt%, geranium 0.00001 wt%, bay milk 0.005 wt%) and cultured for 24 hours and 48 hours, respectively. . The wells were washed 3 times with PBS, and 100 μl of an antibody-POD conjugate solution was added to the wells and left at 37° C. for 1 hour. The wells were washed 3 times with PBS, and 100 μl of a substrate solution (TMB) was added to the wells and left at 25° C. for 30 minutes. 100 μl of 1N H ₂ SO ₄ was put into the well and absorbance was measured at 450 nm using an ELISA reader (Bio-Tek instrument) for the amount of collagen. At this time, the results were derived by calculating the collagen production increasing activity according to the relative difference in absorbance with the DMSO-treated control group. The results are shown in FIGS. 11 and 12 .

Referring to FIGS. 11 and 12 , a higher amount of collagen synthesis was observed when each essential oil was mixed and applied than when treated alone. This indicates that using a mixture of essential oils having collagen synthesis ability can increase the amount of collagen synthesis in dermal dermal fibroblasts compared to when each essential oil is used alone. In particular, the amount of collagen synthesis decreased after 48 hours of treatment with cypress essential oil, lavender essential oil, rosemary essential oil, and geranium essential oil, respectively.

Hereinafter, preferred formulation examples are presented to help the understanding of the present invention. However, the following formulation examples are only provided for easier understanding of the present invention, and the content of the present invention is not limited by the formulation examples.

Formulation example 1: Softening lotion ( skin )

As shown in Table 1 below, softened lotion containing complex essential oil was prepared according to a conventional method. In this case, the compound essential oil was mixed with cypress essential oil: lavender essential oil: rosemary essential oil: geranium essential oil: bay oil in a weight ratio of 9:0.09:4.5:0.009:4.5.

ingredient content (wt%) compound essential oil 0.3 glycerin 5.0 1,3-butylene glycol 3.0 betaine 1.0 allantoin 0.1 EDTA-2Na 0.02 Sodium Hyaluronate Powder 0.05 ethanol 5.0 Octyldodeces-16 0.2 Polyoxyethylene hydrogenated castor oil 0.2 antiseptic appropriate amount Purified water remaining amount Sum 100

Formulation example 2: Nutrient lotion (lotion)

Nutrient lotion containing complex essential oil was prepared according to a conventional method as shown in Table 2 below. In this case, the compound essential oil was mixed with cypress essential oil: lavender essential oil: rosemary essential oil: geranium essential oil: bay oil in a weight ratio of 9:0.09:4.5:0.009:4.5.

ingredient content (wt%) complex essential oil 0.5 Glyceryl Stearate SE 1.5 cetearyl alcohol 1.0 shea butter 1.5 Polysorbate 60 1.3 Sorbitan Stearate 0.5 hydrogenated vegetable oil 1.0 mineral oil 5.0 squalane 3.0 cyclomethicone 2.0 dimethicone 0.8 tocopherol acetate 0.5 Carbomer 0.12 glycerin 5.0 1,3-butylene glycol 3.0 Sodium Hyaluronate Powder 0.05 triethanolamine 0.12 antiseptic appropriate amount Purified water remaining amount Sum 100

Formulation example 3: Massage Cream

As shown in Table 3 below, a massage cream containing complex essential oil was prepared according to a conventional method. In this case, the compound essential oil was mixed with cypress essential oil: lavender essential oil: rosemary essential oil: geranium essential oil: bay oil in a weight ratio of 9:0.09:4.5:0.009:4.5.

ingredient content (wt%) compound essential oil 0.5 Glycerin Monostearate 1.5 cetearyl alcohol 1.5 stearic acid 1.0 Polysorbate 60 1.5 Sorbitan Stearate 0.6 isostearyl isosterate 5.0 squalane 5.0 mineral oil 35.0 dimethicone 0.5 Hydroxyethyl Cellulose 0.12 glycerin 6.0 1,3-butylene glycol 3.0 triethanolamine 0.3 antiseptic appropriate amount Purified water remaining amount Sum 100

Formulation example 4: Essence

As shown in Table 4 below, an essence containing complex essential oil was prepared according to a conventional method. In this case, the compound essential oil was mixed with cypress essential oil: lavender essential oil: rosemary essential oil: geranium essential oil: bay oil in a weight ratio of 9:0.09:4.5:0.009:4.5.

ingredient content (wt%) compound essential oil 0.5 glycerin 6.0 betaine 5.0 PEG 1500 2.0 allantoin 0.1 EDTA-2Na 0.02 Hydrogenated Lecithin 0.6 Hydroxyethyl Cellulose 0.1 Sodium Hyaluronate Powder 0.08 Carboxyvinyl Polymer 0.2 triethanolamine 0.2 ceramide 0.2 Octyldodecanol 3.0 squalane 3.0 Polysorbate 60 0.4 Glyceryl Stearate SE 1.5 antiseptic appropriate amount Purified water remaining amount Sum 100

Formulation example 5: Ointment

As shown in Table 5 below, an ointment containing a complex essential oil was prepared according to a conventional method. In this case, the compound essential oil was mixed with cypress essential oil: lavender essential oil: rosemary essential oil: geranium essential oil: bay oil in a weight ratio of 9:0.09:4.5:0.009:4.5.

ingredient content (wt%) Purified water remaining amount compound essential oil 0.5 glycerin 8.0 butylene glycol 4.0 liquid paraffin 15.0 Carbomer 0.1 Caprylic/Capric Triglycerides 3.0 squalin 1.0 cetearyl glucoside 1.5 Sorbitan Stearate 0.4 cetearyl alcohol 1.0 beeswax 4.0

Claims (9)

A composition for external application to the skin for promoting collagen biosynthesis, comprising as active ingredients a complex essential oil of cypress essential oil, lavender essential oil, rosemary essential oil, geranium essential oil, and bay oil. The method of claim 1, wherein the complex essential oil is mixed with cypress essential oil: lavender essential oil: rosemary essential oil: geranium essential oil: bay oil essential oil in a weight ratio of 500-1500: 8-12: 450-550: 0.5-1.5: 450-550 A composition for external application for skin for promoting collagen biosynthesis, characterized in that. [Claim 3] The composition for external application for skin application for promoting collagen biosynthesis according to claim 1 or 2, wherein the composition contains the complex essential oil in an amount of 0.0001 to 30.0% by weight based on the total weight of the composition. According to claim 1 or 2, wherein the composition for external application to the skin for promoting collagen production is an ointment, gel, cream, patch or spray formulation, characterized in that the composition for external application to the skin for promoting collagen biosynthesis. A cosmetic composition for wrinkle improvement comprising a complex essential oil of cypress essential oil, lavender essential oil, rosemary essential oil, bay oil and geranium essential oil as an active ingredient. The method of claim 5, wherein the complex essential oil is mixed with cypress essential oil: lavender essential oil: rosemary essential oil: geranium essential oil: bay oil essential oil in a weight ratio of 500-1500: 8-12: 450-550: 0.5-1.5: 450-550 A cosmetic composition for wrinkle improvement, characterized in that. The cosmetic composition for improving wrinkles according to claim 5 or 6, wherein the complex essential oil has a collagen synthesis promoting effect. The cosmetic composition for improving wrinkles according to claim 5 or 6, wherein the composition contains the complex essential oil in an amount of 0.0001 to 30.0% by weight based on the total weight of the composition. 7. The method of claim 5 or 6, wherein the wrinkle-improving cosmetic composition is selected from the group consisting of lotion, emulsion, cream, foam, essence, serum, soft water, emulsion, foundation, makeup base, soap, sunscreen cream and sun oil. A cosmetic composition for wrinkle improvement, characterized in that it has one selected formulation.

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