KR102470781B1 - Cosmetic Composition for Reducing Skin Wrinkle - Google Patents

Abstract

The present invention is Pimenta racemosa ( Pimenta racemosa ) as an active ingredient, and relates to a composition for external application for skin and a cosmetic composition for promoting collagen biosynthesis, wherein the composition can further alleviate skin wrinkles by promoting collagen synthesis, and keeps the skin more firm and elastic. effect can be obtained.

Description

Cosmetic composition for reducing wrinkles {Cosmetic Composition for Reducing Skin Wrinkle}

The present invention is Pimenta racemosa ( Pimenta racemosa ) It relates to a composition for external application for skin and a cosmetic composition for promoting collagen production containing an extract.

Skin is the largest tissue in the integumentary system consisting of a plurality of epithelial tissues that protect muscles and organs in the body, and plays an important role in maintaining homeostasis in the body by protecting the inside of the body from the external environment. As a result, the normal function of the skin is deteriorated due to external physical and chemical stimuli, stress, nutritional deficiency, etc., and skin aging phenomena such as loss of elasticity and wrinkle formation are promoted.

Skin aging can be divided into intrinsic age and extrinsic age. The endogenous aging is aging that occurs as age increases, and is aging that occurs when physiological functions of the body are lowered. In addition, the extrinsic aging refers to an aging phenomenon caused by external ultraviolet rays, active oxygen, stress, and the like. Aging of the skin progresses due to these intrinsic and extrinsic aging factors, and as a result, wrinkles are generated on the skin surface, resulting in rough skin texture and reduced elasticity.

According to the mechanism by which wrinkles are formed, collagen, elastin, etc. in the dermis are continuously decomposed and produced. is created

Representative wrinkle reduction and skin aging prevention substances that have been studied and reported so far include retinoic acid, retinol, ascorbic acid, α-hydroxy acid, and the like.

Retinoic acid inhibits lipid per-oxidation and ornithine decarboxylase response. However, it shows a sensitive reaction to ultraviolet rays and has a side effect of reddening of the skin due to high irritation, and its use is limited, and there is a problem in that the effect appears only when continuously used for 6 months or more.

Retinol has a weak anti-wrinkle effect compared to retinoic acid, but is relatively safe and widely used. However, when retinol is used in high concentration, it causes skin irritation, causes skin pigmentation when exposed to ultraviolet rays, and is easily oxidized by oxygen in the air, heat, and light, so there is a problem in stability.

Ascorbic acid is easily oxidized by external environments such as air, moisture, and high temperature, and thus its activity is greatly reduced, so the degree of wrinkle improvement is lowered and discoloration and odor of the formulation are caused.

In addition, α-hydroxy acid is also disclosed as an anti-wrinkle agent, but it is accompanied by skin irritation side effects such as itching, erythema, and sensitivity to ultraviolet rays, and there is a problem in terms of safety of the product.

In addition, cosmetics containing sunscreens, moisturizers, antioxidants, etc. are widely used to prevent wrinkles, but the actual wrinkle improvement effect of these products is insignificant.

On the other hand, essential oil is extracted from the fruit, leaf, flower, stem, bark and root of an existing tree, and is named after the extracted plant. Essential oil extracted from plants is not a single chemical component, but a mixture of monoerpene and phenolics compounds with strong volatile content. The composition of essential oil components differs depending on the genetic characteristics of the extracted plant species, the environment of the cultivation area, and the time of collection. These essential oils do not directly affect the growth and differentiation of plants, but are substances that exhibit various functions, and are widely used in medicine, perfume, and household goods.

For example, Korean Patent Publication No. 2014-0062958 discloses an aroma oil composition and a cosmetic composition having an effect of reducing and preventing body fat or cellulite containing an essential oil fraction extracted from a seshin extract, and a cosmetic composition for preventing and improving depression. are starting

Korean Patent Publication No. 2014-0062958

The present inventors Pimenta racemosa ( Pimenta The present invention was completed by confirming that the extract of racemosa ) promotes collagen production and exhibits a skin wrinkle improvement effect when applied to the skin.

Therefore, the present invention is Pimenta racemosa ( Pimenta racemosa ) as an active ingredient, and to provide a composition for external application for skin for promoting collagen synthesis.

In addition, the present invention is Pimenta racemosa ( Pimenta racemosa ) as an active ingredient to provide a cosmetic composition for improving wrinkles containing an extract as an active ingredient

In order to achieve the above object, the present invention

Pimenta racemosa ( Pimenta racemosa ) It provides a composition for external application for skin for promoting collagen biosynthesis containing an extract as an active ingredient.

Also, the present invention

Pimenta racemosa ( Pimenta racemosa ) It provides a cosmetic composition for improving wrinkles containing an extract as an active ingredient.

Since the composition for external use and the cosmetic composition according to the present invention promote collagen production by acting on fibroblasts in the dermal layer of the skin, they can be usefully used to prevent or improve skin wrinkles, decrease in skin elasticity, or aging.

1 is a graph showing the cytotoxicity results of bay oil on human dermal fibroblasts in Experimental Example 1.
2 is a graph showing the production increasing activity of bay oil for collagen in dermal fibroblasts in Experimental Example 2;

The composition of the present invention as an active ingredient Pimenta racemosa ( Pimenta racemosa ) extract.

In the present invention, the Pimenta racemosa (Pimenta racemosa) The extract is obtained by extracting from various organs or parts, Pimenta racemosa (Pimenta racemosa) It is characterized by extracting by adding a solvent to any one or more selected from leaves, flowers, stems, branches, roots, outposts, and fruits. Leaves are preferably used.

Pimenta racemosa used as a raw material in the present invention racemosa ) Extract is Pimenta racemosa ( Pimenta racemosa ), a leachate obtained by leaching or transfer, or a concentrate obtained by partially or entirely concentrating the leachate, or an extract prepared by drying the concentrate again, and a chemical substance containing the main effect contained in the extract contains itself

The extract of the present invention is prepared according to a conventional method known in the art, that is, using a conventional solvent, distillation extraction, cooling, reflux cooling extraction, hot water extraction, room temperature extraction, warming extraction, ultrasonic extraction , supercritical extraction, and the like.

Specifically, purified water, alcohol having 1 to 6 carbon atoms, glycerin, alkylene glycol having 2 to 6 carbon atoms, alkanes having 1 to 6 carbon atoms, halogenated hydrocarbons having 1 to 6 carbon atoms, alkyl alkanoates having 2 to 6 carbon atoms, and mixtures thereof Extraction solvents selected from may be used. More specifically, an extraction solvent selected from purified water, methanol, ethanol, 1,2-propanediol, glycerin, ethylene glycol, butylene glycol, propylene glycol, dichloromethane, dichloroethane, hexane, ethyl acetate, and mixtures thereof may be used. can At this time, the amount of the extraction solvent is 5 to 30 times the volume of the raw material weight, more preferably 5 to 20 times.

The extraction temperature may be selected by those skilled in the art in various temperature ranges suitable for the extraction method, and may be performed at, for example, 20 °C to 100 °C, but is not limited thereto. In addition, the extraction time differs depending on the extraction method, and a person skilled in the art may select an appropriate extraction time, but is not limited thereto, and may be performed single or multiple times in the range of about 1 hour to several days. The extract obtained by performing the extraction with the primary extraction solvent is obtained in liquid form from which impurities are removed by filtering according to a conventional method, or the obtained liquid extract is concentrated under reduced pressure and / or dried according to a conventional method to obtain a powder form. You can get it.

According to one embodiment of the present invention, the extract is a bay oil obtained by steam distillation of Pimenta racemosa leaves.

In addition, when using the extract according to the present invention as a composition component, it can be used not only in the form of a lyophilized powder, but also in the form of a liquid dispersed or dissolved in water or a common organic solvent.

In addition, the composition according to the present invention may contain, in addition to the above extract components, other components capable of giving a synergistic effect to the main effect, preferably within a range that does not impair the main effect intended by the present invention.

As a result of experiments to determine the collagen synthesis effect of bay oil through Experimental Example 2 according to the present invention, the amount of collagen synthesis increased by 104% or more when treated for 24 hours and 140% or more when treated for 48 hours.

Through these results, the extract of Pimenta racemosa presented in the present invention can be applied as an external composition for skin and a cosmetic composition, and when applied to the skin, by promoting collagen synthesis, skin wrinkles can be further alleviated. It has the effect of keeping the skin firmer and more elastic.

Pimenta racemosa ( Pimenta racemosa ) may be included in an amount of 0.0001 to 30.0% by weight, preferably 0.001 to 30.0% by weight, based on the total weight of the composition for external application. If the content of the compound is less than the suggested range, a clear effect cannot be expected, and conversely, if the content exceeds the suggested range, the increase in effect is insignificant compared to the increase in the content and may cause slight cytotoxicity.

The composition for external application is not particularly limited in its formulation, and may be, for example, a composition for external application having a formulation of body lotion, ointment, gel, cream, patch, or spray. At this time, in the composition for external use of each dosage form, other components other than the extract of Pimenta racemosa described above may be appropriately selected and blended by a person skilled in the art according to the formulation or purpose of use of other external preparations.

In particular, Pimenta racemosa according to the present invention ( Pimenta racemosa ) can be applied as a cosmetic composition containing it as an active ingredient through the collagen synthesis promoting effect.

Pimenta racemosa ( Pimenta The extract of racemosa ) may be included in an amount of 0.0001 to 30.0% by weight, preferably 0.001 to 30.0% by weight, based on the weight of the total cosmetic composition. If the content of the compound is less than the suggested range, a clear effect cannot be expected, and on the contrary, if it exceeds the above range, the increase in the effect is not achieved compared to the increase in the amount contained and may exhibit slight cytotoxicity.

The cosmetic composition is not particularly limited in its dosage form, and for example, lotion, lotion, cream, foam, essence, serum, softening water, lotion, foundation, makeup base, soap, sunscreen cream and sun oil, etc. It can be made into a formulation.

In addition, the composition of each formulation may contain various bases and additives necessary and appropriate for formulation of the formulation, and within the range that does not reduce the effect, nonionic surfactants, silicone polymers, extender pigments, fragrances, preservatives, Bactericides, oxidative stabilizers, organic solvents, ionic or nonionic thickeners, softeners, antioxidants, free radical destroyers, opacifying agents, stabilizers, emollients, silicones, α-hydroxy acids, antifoaming agents, humectants, Vitamins, insect repellents, fragrances, preservatives, surfactants, anti-inflammatory agents, Substance P antagonists, fillers, polymers, propellants, basifying or acidifying agents, or colorants.

Hereinafter, a preferred embodiment is presented to aid understanding of the present invention. However, the following examples are only provided to more easily understand the present invention, and the content of the present invention is not limited by the examples.

Experimental example One: bayou's Confirmation of cytotoxicity

1. Culture of human dermal fibroblasts

Human dermal fibroblasts were purchased from Gibco (Life Technologies, Carlsbad, CA, USA). Cells were cultured at 37°C in DMEM medium (Gibco). The medium was maintained until the attached cells reached 80% confluence and subculture was performed at 80% confluence. Medium was changed every 2 days until confluence. In the subculture, the flask from which the culture medium was removed was washed with phosphate buffer saline (PBS), the cells were dropped with 0.25% trypsin-EDTA (Gibco BRL, Grand Island, NY, USA), and the cell suspension was centrifuged. After that, the number of cells was measured and subcultured three times again in the culture medium.

2. Cytotoxicity measurement

DMEM medium (Gibco) supplemented with 10% serum was added to a 96-well plate, inoculated with 5 X 10 ² human skin fibroblasts, and cultured under 5% CO ₂ and 37 ° C conditions to attach the cells to the culture dish. Next, after culturing in the culture medium from which the serum was removed for 24 hours, it was divided into a negative control group (DMSO treatment) and a bayou treatment group. Bayou was dissolved in DMSO by concentration and treated with 10 μl (treatment concentration 0.00001 wt% to 0.08 wt%).

After the treatment, the cells were cultured for 24 hours at 5% CO ₂ and 37°C. Thereafter, the MTT reagent was added, and after 4 hours, the medium was removed, and 200 μl of DMSO was added to dissolve the formazan crystal, and the absorbance was measured at 570 nm using a microplate reader. The results are shown in Figure 1.

From the results of FIG. 1, it was found that, based on the control group, there was no cytotoxicity at a final concentration of 0.005 wt% or less in the condition treated with bayou, and cell proliferation increased by about 10% at a very low concentration.

Experimental example 2: Effect of increasing the amount of collagen synthesis in dermal fibroblasts

In order to confirm the effect of bay oil on the amount of collagen synthesis in dermal fibroblasts, a collagen measurement experiment was conducted. The dermal fibroblast cell line was added to DMEM medium supplemented with 10% fetal bovine serum, 100 U/ml penicillin, and 100 μg/ml streptomycin, and cultured at 37°C, 5% CO ₂ , conditions. After culturing for 1 day, the culture medium was changed to 0.0005 wt% of bayou, followed by culturing for 24 hours and 48 hours, respectively. The wells were washed three times with PBS, and 100 μl of the antibody-POD conjugate solution was put into the wells and left at 37° C. for 1 hour. The wells were washed three times with PBS, and 100 μl of a substrate solution (TMB) was added to the wells and allowed to stand at 25° C. for 30 minutes. 100 μl of 1N H ₂ SO ₄ was added to the well, and absorbance at 450 nm was measured for the amount of collagen using an ELISA reader (Bio-Tek instrument). At this time, the result was derived by calculating the collagen production increasing activity according to the relative absorbance difference from the control group treated with DMSO, and the results are shown in FIG. 2.

Referring to Figure 2, when the amount of collagen synthesis was measured, when treated with bayou for 24 hours, the amount of collagen synthesis was shown to be about 104%, and the increase was insignificant, whereas, when treated for 48 hours, the amount of collagen synthesis was about 140% increased. This indicates that the effect of collagen synthesis is increased when the bayou is continuously used.

Hereinafter, preferred formulation examples are presented to aid understanding of the present invention. However, the following formulation examples are only provided to more easily understand the present invention, and the content of the present invention is not limited by the formulation examples.

formulation example 1: Softening lotion ( skin )

As shown in Table 1 below, softening lotion containing bay oil was prepared according to a conventional method.

ingredient Content (% by weight) bay oil 0.3 glycerin 5.0 1,3-butylene glycol 3.0 betaine 1.0 allantoin 0.1 EDTA-2Na 0.02 Sodium Hyaluronate Powder 0.05 ethanol 5.0 Octyldodeces-16 0.2 Polyoxyethylene Hydrogenated Castor Oil 0.2 antiseptic Appropriate amount Purified water balance Sum 100

formulation example 2: Nourishing lotion (lotion)

As shown in Table 2 below, nutrient lotion containing bay oil was prepared according to a conventional method.

ingredient Content (% by weight) bay oil 0.5 Glyceryl Stearate SE 1.5 Cetearyl Alcohol 1.0 shea butter 1.5 Polysorbate 60 1.3 Sorbitan Stearate 0.5 hydrogenated vegetable oil 1.0 mineral oil 5.0 squalane 3.0 cyclomethicone 2.0 dimethicone 0.8 Tocopherol Acetate 0.5 carbomer 0.12 glycerin 5.0 1,3-butylene glycol 3.0 Sodium Hyaluronate Powder 0.05 triethanolamine 0.12 antiseptic Appropriate amount Purified water balance Sum 100

formulation example 3 : Massage Cream

As shown in Table 3 below, a massage cream containing bay oil was prepared according to a conventional method.

ingredient Content (% by weight) bay oil 0.5 Glycerin Monostearate 1.5 Cetearyl Alcohol 1.5 stearic acid 1.0 Polysorbate 60 1.5 Sorbitan Stearate 0.6 isostearyl isosterate 5.0 squalane 5.0 mineral oil 35.0 dimethicone 0.5 Hydroxyethyl Cellulose 0.12 glycerin 6.0 1,3-butylene glycol 3.0 triethanolamine 0.3 antiseptic Appropriate amount Purified water balance Sum 100

formulation example 4 : Essence

As shown in Table 4 below, an essence containing bay oil was prepared according to a conventional method.

ingredient Content (% by weight) bay oil 0.5 glycerin 6.0 betaine 5.0 PEG 1500 2.0 allantoin 0.1 EDTA-2Na 0.02 Hydrogenated Lecithin 0.6 Hydroxyethyl Cellulose 0.1 Sodium Hyaluronate Powder 0.08 carboxyvinyl polymer 0.2 triethanolamine 0.2 ceramide 0.2 Octyldodecanol 3.0 squalane 3.0 polysorbate 60 0.4 Glyceryl Stearate SE 1.5 antiseptic Appropriate amount Purified water balance Sum 100

formulation example 5: ointment

As shown in Table 5 below, an ointment containing bay oil was prepared according to a conventional method.

ingredient Content (% by weight) Purified water balance bay oil 0.5 glycerin 8.0 Butylene Glycol 4.0 liquid paraffin 15.0 carbomer 0.1 Caprylic/Capric Triglyceride 3.0 squaline 1.0 Cetearyl Glucoside 1.5 Sorbitan Stearate 0.4 cetearyl alcohol 1.0 beeswax 4.0

Claims (9)

Pimenta racemosa ( Pimenta racemosa ) A composition for external application for skin for promoting collagen biosynthesis containing as an active ingredient bay oil obtained by steam distillation of leaves. delete The composition for external application for skin for promoting collagen biosynthesis according to claim 1, wherein the composition comprises bay oil in an amount of 0.0001 to 30.0% by weight based on the total weight of the composition. The composition for external application for skin according to claim 1, wherein the composition for external application for skin for promoting collagen production is in the form of an ointment, gel, cream, patch or spray. Pimenta racemosa ( Pimenta racemosa ) A cosmetic composition for improving wrinkles containing bay oil obtained by steam distillation of leaves as an active ingredient. delete The cosmetic composition for improving wrinkles according to claim 5, wherein the bay oil has an effect of promoting collagen synthesis. The cosmetic composition for improving wrinkles according to claim 5, wherein the composition comprises bay oil in an amount of 0.0001 to 30.0% by weight based on the total weight of the composition. The method of claim 5, wherein the cosmetic composition for improving wrinkles is one selected from the group consisting of lotion, emulsion, cream, foam, essence, serum, softening water, emulsion, foundation, makeup base, soap, sunscreen cream and sun oil A cosmetic composition for improving wrinkles, characterized in that it has a formulation of.

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