KR20170057580A - Cosmetic Composition for Reducing Skin Wrinkle - Google Patents

Abstract

The present invention relates to a composition for external skin application for promoting collagen biosynthesis containing a Pimenta racemosa extract as an active ingredient, and a cosmetic composition. Since the composition promotes synthesis of collagen, wrinkles on skin can be reduced and keeps skin more firm and elastic.

Description

[0001] Cosmetic Composition for Reducing Skin Wrinkle [

The present invention relates to Pimenta < RTI ID = 0.0 > Racemosa ) and a cosmetic composition for enhancing collagen production.

Skin is the largest tissue in the epidermal system consisting of multiple epithelial tissues that protects the muscles and organs in the body and plays an important role in maintaining the body's homeostasis by protecting the inside of the body from the external environment. As a result, the skin is exposed to external physical and chemical stimuli, stress, nutritional deficiencies, such as the normal function of the skin degraded, elasticity and wrinkles, such as skin aging phenomenon is promoted.

Skin aging can be divided into innate age and extrinsic age. The above-mentioned internal aging is an aging caused by an increase in age, and aging resulting from a decrease in the physiological function of the body. The external aging refers to the aging phenomenon caused by external ultraviolet rays, active oxygen, stress, and the like. These internal and external aging factors aging the skin promote wrinkles on the skin surface, resulting in rough skin texture and reduced elasticity.

According to the mechanism of wrinkle formation, the collagen (collagen) and elastin in the dermis continue to be decomposed and produced. If the supplement after the decomposition is not smooth, the skin loses elasticity and wrinkles .

Retinoic acid, retinol, ascorbic acid, and? -Hydroxy acid are among the representative anti-wrinkle and anti-aging substances reported so far.

Retinoic acid inhibits lipid per-oxidation and ornithine decarboxylase response. However, it is sensitive to ultraviolet rays and has a high irritation, so it has been reported that side effects of redness of the skin have been reported, and its use is restricted.

Retinol is less effective for wrinkle improvement than retinoic acid, but is widely used because of its relatively high safety. However, when retinol is used at a high concentration, skin irritation occurs, skin pigmentation occurs upon exposure to ultraviolet rays, and oxidation is easily caused by oxygen, heat and light in the air.

Ascorbic acid is easily oxidized by the external environment such as air, water, and high temperature, and thus the activity is greatly reduced, thereby lowering the degree of wrinkle improvement and causing discoloration and deterioration of the formulation.

Although α-hydroxy acid is also disclosed as an anti-wrinkle agent, there are side effects of skin irritation such as itching, erythema, and sensitivity to ultraviolet rays, thereby causing a problem of safety of the product.

In addition, cosmetic products containing sunscreen agents, moisturizers, and antioxidants are widely used to prevent wrinkles, but the effect of wrinkles on these products is minimal.

On the other hand, essential oil is extracted from the fruit, leaf, flower, stem, bark and root of the existing tree and named after the extracted plant. Essential oils extracted from plants are a mixture of monoerpene and phenolic compounds which are not a single chemical component but strong volatile components. The genetic characteristics of the extracted plant species and the composition of the essential oil components are different according to the environment and harvesting season in the cultivation area. These essential oils do not directly affect the growth and differentiation of plants, but they have various functions and are widely used in medicines, fragrances, household goods and the like.

For example, Korean Patent Laid-Open Publication No. 2014-0062958 discloses an aroma oil composition and a cosmetic composition having body fat or cellulite reducing and preventing effect including an essential oil fraction extracted from a sesamin extract, and a cosmetic composition for preventing and improving depression Lt; / RTI >

Korean Patent Publication No. 2014-0062958

The present inventors have found that Pimenta Racemosa extract promotes collagen production and is effective for improving skin wrinkles when applied to skin substantially. The present invention has been completed based on this finding.

Thus, the present invention is based on the discovery that Pimenta It is an object of the present invention to provide a composition for promoting collagen synthesis comprising an extract of racemosa as an active ingredient.

The present invention also relates to a process for the preparation of Pimenta < RTI ID = 0.0 > Another object of the present invention is to provide a cosmetic composition for improving wrinkles containing an extract of racemosa as an active ingredient

In order to achieve the above object,

Pimenta The present invention provides a composition for external application for skin for promoting collagen biosynthesis, which comprises an extract of racemosa as an active ingredient.

Also,

Pimenta The present invention provides a cosmetic composition for improving wrinkles containing an extract of Racemosa as an active ingredient.

The external application composition and cosmetic composition according to the present invention act on fibroblasts of the dermal layer to promote collagen production, and thus can be usefully used for preventing or improving skin wrinkles, skin elasticity, or aging.

FIG. 1 is a graph showing the cytotoxicity results of bay oil against human skin fibroblasts in Experimental Example 1. FIG.
FIG. 2 is a graph showing the activity of increasing the production of bay oil against collagen in dermal fibroblasts in Experimental Example 2. FIG.

The composition of the present invention contains, as an active ingredient, Pimenta racemosa extract.

In the present invention, the Pimenta racemosa extract is obtained by extracting from various organs or parts and is characterized in that a solvent is added to at least one selected from leaves, flowers, stems, branches, roots, outposts and fruits of Pimenta racemosa . Leaves are preferably used.

In the present invention, Pimenta < RTI ID = 0.0 > racemosa extracts were obtained from Pimenta racemosa , or a concentrate obtained by partially or entirely concentrating the leach solution, or an extract obtained by drying the concentrate again, and a chemical substance exhibiting the main effect contained in the extract liquid Including itself.

The extract of the present invention can be isolated and purified by conventional methods known in the art, that is, by using conventional solvents such as distillation extraction, cold extraction, reflux cooling extraction, hot water extraction, room temperature extraction, , Supercritical extraction, and the like.

Specific examples thereof include purified water, alcohols having 1 to 6 carbon atoms, glycerin, alkylene glycols having 2 to 6 carbon atoms, alkanes having 1 to 6 carbon atoms, halogenated hydrocarbons having 1 to 6 carbon atoms, alkylalkanoates having 2 to 6 carbon atoms, and mixtures thereof Can be used. More specifically, extraction solvents selected from purified water, methanol, ethanol, 1,2-propanediol, glycerin, ethylene glycol, butylene glycol, propylene glycol, dichloromethane, dichloroethane, hexane, ethyl acetate and mixtures thereof . In this case, the amount of the extraction solvent used is 5 to 30 times the volume of the raw material, more preferably 5 to 20 times.

The extraction temperature can be varied by a person skilled in the art in a variety of temperature ranges suitable for the extraction method, for example, but not limited to, from 20 캜 to 100 캜. In addition, the extraction time differs depending on the extraction method, and a person skilled in the art can adopt an appropriate extraction time, and can be performed in a single or multiple cycles in a range of about 1 hour to several days, though it is not limited thereto. The extract obtained by performing the extraction with the primary extraction solvent may be obtained in the form of a liquid in which impurities are removed by filtration according to a conventional method, or the obtained liquid form extract may be concentrated under reduced pressure and / Can be obtained.

According to one embodiment of the present invention, the extract is bay oil obtained by steam distillation of Pimenta racemosa leaves.

In addition, when the extract according to the present invention is used as a constituent component, it can be used not only in the form of a lyophilized powder, but also in the form of a liquid dispersed or dissolved in water or a common organic solvent.

In addition, the composition according to the present invention may contain, in addition to the above-mentioned extract components, other components which can give a synergistic effect to the main effect, within a range not impairing the intended main effect of the present invention.

Experimental Example 2 according to the present invention was conducted to examine the effect of collagen synthesis on bay oil. As a result, the amount of collagen synthesis increased by more than 104% in 24 hours treatment and 140% in 48 hours treatment.

From these results, it can be seen that the extract of Pimenta racemosa proposed in the present invention can be applied as a composition for external application for skin and a cosmetic composition, and promotes collagen synthesis when applied to skin, And the effect of keeping the skin more firm and elastic can be obtained.

Pimenta racemosa may contain from 0.0001 to 30.0% by weight, preferably from 0.001 to 30.0% by weight, based on the weight of the total external preparation composition. If the content of the compound is below the recommended range, no significant effect can be expected. On the contrary, if the content exceeds the recommended range, the increase of the effect is insignificant and may cause slight cytotoxicity compared with the increase of the content.

The external preparation composition is not particularly limited in its formulation and may be, for example, an external preparation composition having a formulation of a body lotion, ointment, gel, cream, patch or spray. At this time, in the composition for external application for each formulation, components other than the extract of Pimenta racemosa may be selected by those skilled in the art according to the formulation or purpose of use of the other external preparation.

In particular, Pimenta < RTI ID = 0.0 > racemosa extract can be applied as a cosmetic composition containing it as an active ingredient through promoting collagen synthesis.

Pimenta racemosa may be contained in an amount of 0.0001 to 30.0% by weight, preferably 0.001 to 30.0% by weight, based on the total weight of the cosmetic composition. If the content of the compound is below the recommended range, no significant effect can be expected. On the other hand, if the content of the compound exceeds the above range, the effect is not increased as compared with the content of the compound, and slight cytotoxicity may be exhibited.

The composition of the cosmetic composition is not particularly limited in its formulation and may be in the form of a lotion such as a lotion, a milky lotion, a cream, a foam, an essence, a serum, a soft water, an emulsion, a foundation, a makeup base, a soap, ≪ / RTI >

In addition, the compositions of each formulation may contain various bases and additives necessary for formulation of the formulation, and may contain non-ionic surfactants, silicone polymers, extender pigments, flavorings, preservatives, But are not limited to, disinfectants, oxidative stabilizers, organic solvents, ionic or nonionic thickeners, plasticizers, antioxidants, free radical scavengers, opacifiers, stabilizers, emollients, silicones, A preservative, a surfactant, an anti-inflammatory agent, a substance P antagonist, a filler, a polymer, a propellant, a basicizing or acidifying agent, or a coloring agent.

Hereinafter, preferred embodiments of the present invention will be described in order to facilitate understanding of the present invention. However, the following examples are provided to further understand the present invention, and the present invention is not limited by the examples.

Experimental Example  One: Bay  Cytotoxicity check

1. Culture of human skin fibroblasts

Human dermal fibroblast was purchased from Gibco (Life Technologies, Carlsbad, Calif., USA). The cells were cultured in DMEM medium (Gibco) at 37 ° C. The medium was maintained until the adhered cells were confluent at 80% and subcultured at 80% confluence. The medium was replaced every two days until confluence. For the subculture, the flask in which the culture solution had been removed was washed with phosphate buffered saline (PBS), and the cells were suspended in 0.25% trypsin-EDTA (Gibco BRL, Grand Island, NY, USA) After that, the number of cells was measured and subcultured again in the culture solution three times.

2. Cytotoxicity measurement

DMEM medium (Gibco) supplemented with 10% serum was added to each well of a 96-well plate, human fibroblasts were inoculated with 5 x 10 ² cells, cultured at 5% CO ₂ and 37 ° C, Then, the cells were cultured in a serum-free medium for 24 hours, and then divided into negative control (DMSO treatment) and bee treatment. The beads were dissolved in DMSO at a concentration of 10 μl (treatment concentration 0.00001 wt% to 0.08 wt%).

After the treatment, the cells were cultured at 5% CO ₂ and 37 ° C for 24 hours. After 4 hours, MTT reagent was added and the medium was removed. 200 μl of DMSO was added to dissolve the formazan crystal, and the absorbance was measured at 570 nm with a microplate reader. The results are shown in Fig.

From the results shown in FIG. 1, it can be seen that the cells are not cytotoxic at a final concentration of 0.005 wt% or less, and that the cell proliferation is increased by about 10% at a very low concentration when the control group is used as a reference.

Experimental Example  2: Increased amount of collagen synthesis in dermal fibroblasts

To investigate the effect of bay oil on the amount of collagen synthesis in dermal fibroblasts, a collagen measurement experiment was conducted. Dermal fibroblast cells were added to DMEM medium supplemented with 10% fetal bovine serum, 100 U / ml penicillin, and 100 μg / ml streptomycin, and cultured at 37 ° C and 5% CO ₂ . After 1 day of culture, the medium was changed to 0.0005 wt% of bee culture medium and cultured for 24 hours and 48 hours, respectively. The wells were washed 3 times with PBS, and 100 쨉 l of antibody-POD conjugate solution was added to the wells and left at 37 째 C for 1 hour. The wells were washed three times with PBS, and 100 μl of the substrate solution (TMB) was added to the wells and left at 25 ° C for 30 minutes. 1N H ₂ SO ₄ was added to the well and the absorbance of the collagen was measured at 450 nm using an ELISA reader (Bio-Tek instrument). At this time, the activity of increasing collagen production was calculated according to the difference in absorbance relative to the control group treated with DMSO, and the results are shown in Fig.

2, when the degree of collagen synthesis was measured, the amount of collagen synthesis was about 104% when the bean curd was treated for 24 hours, whereas the amount of collagen synthesis was about 140% when treated for 48 hours. Respectively. This indicates that the collagen synthesis effect is increased when the bean is continuously used.

Hereinafter, a preferred formulation example is provided to facilitate understanding of the present invention. However, the following formulation examples are provided only for a better understanding of the present invention, and the present invention is not limited by the formulation examples.

Formulation Example  1: Softening longevity ( skin )

The softening water containing bay oil was prepared according to a conventional method as shown in Table 1 below.

ingredient Content (% by weight) Bay oil 0.3 glycerin 5.0 1,3-butylene glycol 3.0 Betaine 1.0 Allantoin 0.1 EDTA-2Na 0.02 Sodium hyaruronate powder 0.05 ethanol 5.0 Octyldodec-16 0.2 Polyoxyethylene hardened castor oil 0.2 antiseptic Suitable amount Purified water Balance Sum 100

Formulation Example  2: Nourishing lotion (lotion)

A nutrition lotion containing bay oil was prepared according to a conventional method as shown in Table 2 below.

ingredient Content (% by weight) Bay oil 0.5 Glyceryl stearate SE 1.5 Cetearyl alcohol 1.0 Sheer butter 1.5 Polysorbate 60 1.3 Sorbitan stearate 0.5 Hardened vegetable oil 1.0 Mineral oil 5.0 Squalane 3.0 Cyclomethicone 2.0 Dimethicone 0.8 Tocopheryl acetate 0.5 Carbomer 0.12 glycerin 5.0 1,3-butylene glycol 3.0 Sodium hyaruronate powder 0.05 Triethanolamine 0.12 antiseptic Suitable amount Purified water Balance Sum 100

Formulation Example  3: Massage cream

A massage cream containing bay oil was prepared according to a conventional method as shown in Table 3 below.

ingredient Content (% by weight) Bay oil 0.5 Glycerin monostearate 1.5 Cetearyl alcohol 1.5 Stearic acid 1.0 Polysorbate 60 1.5 Sorbitan stearate 0.6 Isostearyl isoserate 5.0 Squalane 5.0 Mineral oil 35.0 Dimethicone 0.5 Hydroxyethylcellulose 0.12 glycerin 6.0 1,3-butylene glycol 3.0 Triethanolamine 0.3 antiseptic Suitable amount Purified water Balance Sum 100

Formulation Example  4: essence

Essences containing bay oil were prepared according to the conventional method as shown in Table 4 below.

ingredient Content (% by weight) Bay oil 0.5 glycerin 6.0 Betaine 5.0 PEG 1500 2.0 Allantoin 0.1 EDTA-2Na 0.02 Hydrogenated lecithin 0.6 Hydroxyethylcellulose 0.1 Sodium hyaruronate powder 0.08 Carboxyvinyl polymer 0.2 Triethanolamine 0.2 Ceramide 0.2 Octyldodecanol 3.0 Squalane 3.0 Polysorbate 60 0.4 Glyceryl stearate SE 1.5 antiseptic Suitable amount Purified water Balance Sum 100

Formulation Example  5: Ointment

Ointment containing bay oil was prepared according to a conventional method as shown in Table 5 below.

ingredient Content (% by weight) Purified water Balance Bay oil 0.5 glycerin 8.0 Butylene glycol 4.0 Liquid paraffin 15.0 Carbomer 0.1 Caprylic / capric triglyceride 3.0 Squueline 1.0 Cetearyl glucoside 1.5 Sorbitan stearate 0.4 Cetearyl alcohol 1.0 Wax 4.0

Claims (9)

Pimenta Racemosa extract as an active ingredient for promoting collagen biosynthesis. The composition for external application for skin for promoting collagen biosynthesis according to claim 1, wherein the extract is bay oil. The composition for external application for skin for promoting collagen biosynthesis according to claim 1 or 2, wherein the composition comprises the extract in an amount of 0.0001 to 30.0% by weight based on the total weight of the composition. The composition for external application for skin for promoting collagen biosynthesis according to claim 1 or 2, wherein the composition for promoting collagen production is an ointment, a gel, a cream, a patch or a spray. Pimenta A cosmetic composition for improving wrinkles comprising an extract of Racemosa as an active ingredient. The cosmetic composition for wrinkle improvement according to claim 5, wherein the extract is bay oil. The cosmetic composition for improving wrinkles according to claim 5 or 6, wherein the extract has an effect of promoting collagen synthesis. The cosmetic composition for wrinkle improvement according to claim 5 or 6, wherein the composition contains the extract in an amount of 0.0001 to 30.0% by weight based on the total weight of the composition. The cosmetic composition for wrinkle improvement according to claim 5 or 6, wherein the wrinkle improving cosmetic composition is at least one selected from the group consisting of lotion, milky lotion, cream, foam, essence, serum, soft water, emulsion, foundation, makeup base, soap, sunscreen cream, Wherein the cosmetic composition has one type of formulation selected.

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