KR101425031B1 - Cosmetic composition for anti-irritation and skin moisturization containing Citrus junos Siebold seed oil and Mangifera Indica seed oil - Google Patents

Abstract

The present invention relates to a cosmetic composition for alleviating skin irritation and moisturizing skin containing a yuza seed and a mango seed oil, wherein the mixed oil of citrus seed and mango seed is contained in an amount of 0.01 to 10 wt% A cosmetic composition for skin moisturization and skin irritation is provided. The yuza seed and mango seed oil of the present invention has skin moisturizing and anti-inflammatory activity and has an efficacy of greatly suppressing skin irritation caused by skin irritating ingredients such as retinol, and thus has excellent efficacy as a cosmetic composition for relieving skin irritation.

Description

TECHNICAL FIELD The present invention relates to a cosmetic composition for skin irritation and skin moisturization containing citrus seed oil and mango seed oil and a cosmetic composition for skin irritation and skin moisturization containing citrus junos siebold seed oil and mangifera indica seed oil,

The present invention relates to a cosmetic composition for alleviating skin irritation and skin moisturizing containing a mixture oil of citron and mango seeds. More specifically, the present invention relates to a cosmetic composition for skin irritation, , An emulsifier, an antiseptic, a fragrance or other functional substance, or skin irritation caused by irradiation with ultraviolet rays, and is excellent in skin moisturizing effect.

The skin of the human body is an important organ that protects the body from external stimuli. The surface of the skin is composed of the horny layer covering the sebaceous glands secreted from the sebaceous glands and sweat glands, which always replenishes insufficient moisture and remains in a shiny healthy state. In addition, skin protects skin from ultraviolet rays, and plays an important role in the body, such as performing internal temperature control function. Skin is a periodically active organ that causes extinction and production as compared to other organs, and serves to protect physical protection and body water loss. It is ideal when the water content of the stratum corneum is 20%, but when the moisture is lost, the cornea is eliminated and it is easy to be exposed to the outside, causing irritation and skin diseases. Dry skin has weakened immune function, and it is easy to cause allergies such as skin rash, itching, and sting as well as atopic dermatitis. Therefore, it is important to prevent moisture loss of the skin.

Cosmetics that are used to protect such skin and to strengthen the barrier and improve skin are mixed with various ingredients. Soluble agents, emulsifiers, preservatives, fragrances, sunscreen agents and other functional active ingredients, It is known to cause irritation or inflammation, allergies, erythema, edema, itching, and the like. Therefore, there is a growing interest in materials that alleviate the stimulation of cosmetics.

Accordingly, various methods for mitigating skin irritation have been attempted. For example, attempts have been made to remove substances that cause skin irritation. However, such a study is limited because the functional raw material itself acts as a stimulating source, and there is a need to use a substance having the effect of reducing stimulation and reducing side effects while maintaining the effect of the functional raw material.

For example, Korean Patent Publication No. 2009-0073632 discloses a cosmetic composition for relieving irritation and alleviating skin irritation containing a extract of Yedok tree and licorice extract, A composition for relieving skin irritation caused by erythema, stinging or itching, which contains a mixture of ginseng, cinnamon, saururus and turmeric as an active ingredient '.

The present inventors have found that when a substance having an effect of alleviating skin irritation caused by ultraviolet rays or a stimulant component of cosmetics is searched, an excellent skin irritation mitigation effect is obtained when a mixture of citron seed oil and mango seed oil is used, And thus the present invention has been completed.

Accordingly, it is an object of the present invention to provide a cosmetic composition for alleviating skin irritation, which comprises a mixture oil of citron seed and mango seed as an active ingredient.

It is another object of the present invention to provide a cosmetic composition for skin moisturizing which comprises an oil of citron seed oil and a mango seed oil as an active ingredient.

According to the present invention, there is provided a cosmetic composition for alleviating skin irritation comprising 0.01 to 10% by weight of a mixture of citrus bean and mango seed oil based on the total weight of the cosmetic composition. At this time, the citron seed oil and the mango seed oil mixture are prepared by mixing citron seed oil and mango seed oil at a weight ratio of 1:10 to 10: 1, more preferably 1: 1. The cosmetic composition of the present invention alleviates skin irritation caused by ultraviolet rays, and is also excellent in relieving skin irritation caused by retinol, which is a typical functional ingredient. Therefore, it is possible to alleviate the skin irritation caused by retinol while maintaining the activity of retinol by adding the citron seed oil and the mango seed oil to the cosmetic containing retinol.

According to another aspect of the present invention, there is provided a cosmetic composition for skin moisturizing comprising 0.01 to 10% by weight of a mixture of citrus seed and mango seed oil based on the total weight of the cosmetic composition.

Wherein the citron seed oil and the mango seed oil are obtained by separating seeds from citrus fruits and mangos and washing the seeds with water; Drying the washed seed to adjust the moisture contained in the seed to within the range of 5 to 20 wt%; Pulverizing the dried seed with a pulverizer and pulverizing the dried seed; Applying steam to the pulverized seed powder so that the seed powder is humidified and heated; And warming and humidifying the seed powder by pressing and milking and filtering with a filter to obtain a pure oil.

The cosmetic composition may be prepared in a conventional formulation and may be in the form of a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nutrition lotion, a massage cream, And may be made into any one form selected from the group consisting of a pack, a soap, a shampoo, a cleansing foam, a cleansing lotion, a cleansing cream, a body lotion, a body cleanser, a latex, a press powder, a loose powder and an eye shadow.

The cosmetic composition of the present invention is excellent in skin moisturizing effect, anti-inflammatory activity and skin irritation alleviating effect, and the cosmetic composition containing it as an active ingredient has skin moisturizing effect and skin inflammation reaction induced by ultraviolet rays and skin irritation The effect of mitigating the problem is very excellent.

Hereinafter, the present invention will be described in more detail.

Citron seeds of the invention citron (Citrus As a seed of Junos , Yuzu is originated from China and spread to Korea, and the inside and the outside are well separated and become mature and become thick and bright yellow. Especially using the essential oil component of the skin, it is used in beverages and spices rather than pulp. Limonene is an antimicrobial effect in essential oils. Phenol and flavonoids are antioxidant, and they are used as folk remedies for detoxification, intoxication and cold. Yuza is rich in natural and essential oils and contains amino acids, free sugars and organic acids in the pulp. Despite the fact that it contains beneficial essential oils, Yuza has not been used vigorously until now. Mr. Yuza is generated more than 1,800 tons a year, and there is a need to broaden the scope of use by various methods using it.

Mango seed of the present invention, mango (Mangifera indica ) seeds. The leaves of the mangoes are slanting, thick and thin, with a flat edge, no hair, and a length of 10 to 15 cm. Flowers are bloomed in April and April in Malaysia and Taiwan. They are dioecious, hanging at the tip of the branch, and have a reddish white color. Fruit is wide egg shape, 3 ~ 25cm long, 1.5 ~ 10cm butterfly, yellowish when ripened, fleshy yellow and juicy. There is one seed in the fruit and it is used as a medicine or it is edible. In India, bark is used to treat internal bleeding, dysentery, and throat. Bark, flower, and resin are used as medicines.

In the present invention, citrus seed oil and mango seed oil, which are active ingredients, can be prepared by the following method.

First, the seeds are separated from citron and mangoes and the separated seeds are washed with water. The washed seeds are dried to adjust the moisture contained in the seeds to within the range of 5 to 20 wt%, and the dried seeds are pulverized by a pulverizer to be pulverized. Then, steam at a high temperature is added to the pulverized seed powder so that the seed powder is humidified and heated. At this time, the steam application time can be adjusted within a range of about 5 minutes to 30 minutes. If excessive humidification is performed, the fiber of the seed powder adsorbs moisture and may become bulky as it becomes bulky. Through this humidification and warming process, the oil in the seed is melted by the hot steam and the milking process can be smoothly performed. The thus heated and humidified seed powder is pressed and milked. The seed oil extracted during the milking process is filtered with a filter to obtain a pure oil. By such a method, seed oil with high purity can be produced with high efficiency.

In the cosmetic composition of the present invention, the citron seed oil and the mango seed oil are mixed at a weight ratio of 1:10 to 10: 1, preferably 1: 1. The mixed oil may be prepared by preparing yuza seed and mango seed oil followed by mixing, or by mixing yuzu seed and mango seed followed by extraction.

The citron seed oil and the mango seed oil are contained in an amount of 0.01 to 10% by weight based on the total weight of the cosmetic composition. The yuzu and mango seed oil inhibits the expression of tumor necrosis factor (TNF-α) by irradiation with ultraviolet rays, and inhibits skin irritation caused by skin irritants such as retinol, which is one of components contained in functional cosmetics It was confirmed that the effect of alleviating is excellent, and the skin moisturizing effect is also possessed.

Accordingly, the cosmetic composition for relieving irritation comprising the citron seed oil and the mango seed oil of the present invention has an effect of improving skin irritation and skin irritation induced by ultraviolet rays in addition to skin moisturizing effect, Prevention and improvement of dry disease, anti-inflammation, skin trouble prevention or improvement.

It can also be prepared in the form of adjuvants which can be applied topically or systemically, which are conventionally used in the field of dermatology by containing a dermatologically acceptable medium or base. The present invention is also directed to a composition comprising an organic solvent, a solubilizing agent, a thickening and gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizer, a foaming agent, a fragrance, a surfactant, water, an ionic or nonionic emulsifier, Adjuvants such as chelating agents, preservatives, vitamins, blocking agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents.

The cosmetics manufactured from the cosmetic composition containing the citron seed oil and the mango seed oil as an active ingredient of the present invention may be in the form of a lotion, a softener, a milk lotion, a moisturizing lotion, a nutrition lotion, a massage cream, a nutrition cream, a moisturizing cream, It can be applied to formulations such as nutritional essence, pack, soap, shampoo, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, milky lotion, press powder, loose powder, eye shadow and the like.

[Example]

Hereinafter, the constitution and effects of the present invention will be described in more detail with reference to the following examples and test examples. However, these examples and test examples are provided for illustrative purposes only in order to facilitate understanding of the present invention, and the scope and scope of the present invention are not limited by the following examples.

Manufacturing example  1: Production of yuzu seed oil

100 g of yuzu seeds collected from citrus fruit were collected and washed, and the seeds were dried in shade for 3 days to adjust the water content to 15 wt%, and the seeds were pulverized to prepare a powder. After steam was applied on the steamer for 30 minutes, the powder was put into a hydraulic milking machine to extract seed oil, and the oil was filtered through a filter to produce yuza seed oil.

Manufacturing example  2: Manufacture of mango seed oil

100g of mango seed collected from mango fruit was collected and washed, and seeds were dried in shade for 3 days to adjust the moisture content to 15 wt%, followed by pulverization of seeds to prepare powder. After steam was applied on the steamer for 30 minutes, the powder was put in a hydraulic milking machine to extract seed oil, and the oil was filtered through a filter to produce mango seed oil.

Example  1: Manufacture of mixed oil of yuzu and mango seeds

The yuza seeds and the mango seed oil prepared in Preparation Examples 1 and 2 were mixed in a weight ratio of 1: 1 to prepare a mixed oil.

Test Example  1: Cytotoxicity test

A cell toxicity test was conducted by confirming the cell growth rate of the yuzu seed oil and the mango seed oil produced before the efficacy test and calculating the appropriate test sample concentration and confirming whether the concentration to be used had an effect on safety .

The cells used were HaCaT (Human Skin Keratinocyte cell line), Dulbecco's modified Eagle's (Sigma) supplemented with 5% CO ₂ , 10% Fetal bovine serum (FBS, Lonza) and 50 μg / mL streptomycin medium (DMEM, Invitrogen). After the culture, the medium was removed, and the mixed oil prepared in Example 1 was diluted with dimethyl sulfoxide to a final concentration of 100, 500, 1000 and 5000 μg / ml. The diluted solution was cultured and cultured for 24 hours. MTT (100 μl) was added to each well (3 mg / ml), followed by further incubation for 4 hours. After removing the supernatant, 150 μl of dimethylsulfoxide was added, and the resulting formazan was shaken by shaking for 30 minutes. Absorption was measured at 540 nm using a multimicroplate reader (Molecular device Spectra max 190). Cell viability was calculated according to the following equation and the results are shown in Table 1 below.

Cell survival rate (%) = absorbance of sample-added group / absorbance of control group 100

Sample concentration
(占 퐂 / ml) Cell survival rate (%) Citron seed oil Mango seed oil Yuzu seed and mango seed mixed oil Control 100 100 100 100 100 500 100 100 100 1000 100 100 100 5000 99.50 99.40 99.52

As can be seen from the results of Table 1, it was confirmed that the cell survival rate of 99% or more of all the samples to which yuzu seed oil, mango seed oil, yuza seed and mango seed oil were applied did not affect cytotoxicity, Yuza seed oil and mango seed oil were found to be safe.

Test Example  2: Stimulation relaxation test

       To investigate the anti-inflammatory activity of yuza seed oil, mango seed oil, yuza seed oil and mango seed oil of Example 1, the effect on the production of tumor necrosis factor (TNF-α), one of the early inflammatory molecules, was examined.

The formation of pro-inflammatory cytokines such as TNF-a leads to the process of converting arachidonic acid into prostaglandin (prostaglandin) and NO formation due to the activity of phospholipase A2 (phospholipase A2) . The cells used were HaCaT (Human Skin Keratinocyte cell line), Dulbecco's modified Eagle's (Sigma) supplemented with 5% CO ₂ , 10% Fetal bovine serum (FBS, Lonza) and 50 μg / mL streptomycin medium (DMEM, Invitrogen). After incubation, the cells were stressed by UV irradiation for 1 hour. As a control, UV-irradiated cells were used without sample treatment.

For RNA analysis, total RNA in cells was extracted from cell culture using Trizol reagent (Invitrogen, USA). The purity and integrity of the RNA was confirmed by A260 nm / A280 nm ratio measurement, and the RNA yield was measured by absorbance at 260 nm. For cDNA synthesis, 3 μg of total RNA was added at 25 ° C. for 10 minutes with addition of Oligo dT 15 (500 ng / μl) primer, dNTP (10 mM), RTase inhibitor (40 U / μl) and Powerscript II RTase (Clontech, USA) The cDNA was synthesized by primer annealing at 42 ° C for 60 min and RTase denaturation at 95 ° C for 5 min. To amplify TNF-α and GAPDH from cDNA, 3 μl of cDNA, 5 μl of 10 × taq polymerase buffer, 2 μl of 10 mM dNTP, 2 μl of each 10 pmol primer and 0.5 μl of taq polymerase were mixed and adjusted to 50 μl by adding distilled water Lt; / RTI > The primer sequences are shown in Table 2. The PCR product was electrophoresed on 1% agarose gel and analyzed with an image analyzer (UGEN, U: Genius). The density of each band was measured using a density measurement program (Gene Tools from Syngene) Respectively.

Gene  Primer sequence GAPDH Forward    5 '- AAC GAA TTT GGT CGA ACA GC-3' Reverse    5 '- TGA GGA GGG ATT CAG TG - 3' TNF-a Forward    5 '- CAG AGG GAA GAG TTC CCC AG - 3' Reverse    5 '- CCT TGG TCT GGT AGG AGA CG-3'

The results are shown in Table 3 below.

TNF-α expression (%) Unchecked UV irradiation 0.00% 0.00% 0.01% 0.05% 0.10% 0.50% Blank 6.18 100.00 - - - - Citron seed oil - - 53.5 29.31 13.32 9.52 Mango seed oil - - 59.61 37.68 23.85 15.3 Yuzu seed and mango seed mixed oil - - 43.86 23.63 11.20 7.31 α-Bisabolol - - - - - 28.75

As shown in Table 3, the expression of tumor necrosis factor (TNF-α), an inflammatory reaction-induced factor induced by UV, in citron seed oil, mango seed oil, yuza seed oil and mango seed oil according to the present invention Dependent inhibition of the inflammatory response. Therefore, it can be confirmed that the skin inflammation reaction is effectively suppressed in yuzu seed oil, mango seed oil, citron seed oil and mango seed oil, and thus, the effect of alleviating skin irritation and skin troubles is also excellent.

Test Example  3: Reduction of skin irritation by retinol

The human skin cell HaCaT (Human Skin Keratinocyte cell line) was cultured in a T-75 flask (Falcon, USA) with DMEM (Sigma) medium until it grew to about 80% Plate (Falcon, USA) for 12 hours until 1 × 10 ⁵ cfu / well. After culturing, the cells were completely adhered to each other through a microscope to confirm whether they were growing well, and the following experiment was conducted using 0.005% retinol as a skin cell stimulating agent. At the concentration of retinol, normal skin cells generally all die. 200 쨉 l of 0.005% retinol-containing DMEM (Sigma) medium was added to each of 96 wells of the plate cultured with HaCaT cells. Then, the yuza seed and the mango seed mixed oil prepared in Example 1 were added at 0.01, 0.05, 0.1 And 0.5%. In the control group, retinol and yuzu seed oil, mango seed oil, yuza seed and mango seed oil were not added. As a control group, only retinol was added, retinol was added to yuza seed oil, mango seed oil, Yuzu seed and mango seed oil were separately prepared.

After 12 hours from the addition of the test substance, 10 쨉 l of MTT reagent was added to each well in order to compare the survival rate of the cells. The MTT reagent was allowed to stand for 4 hours, and then the cells were cultured in an ELISA instrument (Molecular Devices) And the cell viability was measured by the following equation. The results are summarized in Table 4 below.

Cell survival rate (%) = [(absorbance of control - absorbance of test substance) / absorbance of control] × 100

Sample concentration (%) Cell survival rate (%) Citron seed oil
+ Retinol Mango seed oil
+ Retinol Yuzu seed and mango seed mixed oil + retinol Retinol 0.01 28.67 27.82 46.80 Death of all cells 0.05 38.33 41.22 62.35 0.1 67.43 64.51 88.94 0.5 82.54 85.63 94.60

As shown in the results of Table 4 above, the yuza seed and the mango seed oil have excellent effects of inhibiting retinol-induced skin cell death, compared to each of citron seed oil and mango seed oil. That is, the mixed oil of yuzu and mango seeds showed excellent effect of relieving skin irritation caused by retinol, a skin irritant.

Example  2: emulsion  Base manufacturing

For the clinical test for the skin moisturizing effect and the effect of suppressing the erythema induced from ultraviolet rays of the cosmetic composition containing the yuzu seed oil, the mango seed oil, the yuza seed and the mango seed oil of the present invention, To prepare an emulsion base.

Comparative Example  1 to 3: emulsion  Base manufacturing

An emulsion base containing citron seed oil or mango seed oil or containing neither the citron seed oil nor the mango seed oil of the present invention was prepared as a comparative example as shown in Table 5 below.

ingredient weight% Example 2 Comparative Example 1 Comparative Example 2 Comparative Example 3 glycerin 3.00 3.00 3.00 3.00 Disodium iodide 0.02 0.02 0.02 0.02 Polyglyceryl-3-methylglucoside distearate 1.50 1.50 1.50 1.50 Cetearyl alcohol 0.50 0.50 0.50 0.50 Caprylic / capric triglyceride 7.00 7.00 7.00 7.00 Polyacrylamide & C13-14 Isoparaffin & Laureth-7 0.60 0.60 0.60 0.60 Citron seed oil + mango seed oil (Example 1) 0.5 - - - Mango seed oil - 0.5 - - Citron seed oil - - 0.5 - Incense, preservative a very small amount a very small amount a very small amount a very small amount Purified water Balance Balance Balance Balance system 100 100 100 100

Test Example  4: Moisture applied to human body Possessive  evaluation

In order to evaluate the skin moisturizing effect of the cosmetic composition containing citron seed oil, mango seed oil, citron seed oil and mango seed oil of the present invention, the test substances of Example 2 and Comparative Examples 1 to 3 were applied The water retention capacity of the skin was measured as follows.

The moisture retention capacity was measured by applying a predetermined amount (0.01 g / 4 cm 2) of 20 g / 4 cm 2 to the inside of each of the 20 testees of Example 2 and Comparative Examples 1 and 3 in a constant temperature and humidity room having an indoor temperature of 22 ° C. and a relative humidity of 45% The moisture content of the skin was measured before 1 hour of application and 2 hours after application. The measuring instrument used was a moisture meter (corneometer CM820, Courage + Khazaka, Cologne, Germany) which measures the moisture content of the skin according to the moisture content of the skin. The results are shown in Table 6 below.

division Example 2 Comparative Example 1 Comparative Example 2 Comparative Example 3 Skin electrical conductivity before application 50 50 50 50 Skin electrical conductivity after 1 hour of application 79 69 70 58 Skin electrical conductivity after 2 hours of application 88 72 78 53

From Table 6, the skin conductivity of the emulsion type containing the citron seed oil and the mango seed oil mixed oil of the present invention is higher than that of the comparative examples. As a result, it can be seen that the material having moisture retention ability is a material having moisturizing effect.

Test Example  5: Human skin Patch test

In order to confirm the stimulation-relieving effect and skin safety of the cosmetic composition containing the citron seed oil and the mango seed oil of the present invention, no skin irritation was observed in the past, Twenty women underwent a human skin patch test. 5.0% of lactic acid, which is a skin stimulant, was added to Example 2 of the present invention and Comparative Examples 1 to 3 and used for the test. First, the test area was wiped with 70% ethanol and then dried. 15 μg of the prepared test substance was dripped into a Finn chamber (100 × 10, EPITEST, Finland), and the test piece was hermetically sealed to the inside of the forearm. After 24 hours of exposure, the patches were removed and the test area was marked with a pen. After 1 hour and 24 hours after the labeling, the test site was observed using a magnifying glass (8MC-150, DAZOR, USA) to observe erythema and edema. The skin response was determined according to the rules of the International Contact Dermatitis Research Group (ICDRG), and the mean skin response (response rate) was determined according to the following formula, and the results are shown in Table 8. Evaluation criteria and scores of skin reactions are shown in Table 7.

Mean response rate = (score × number of reactions × 100 × ½) / [3 (maximum score) × total number of subjects (n)]

Symbol score Evaluation standard - 0 No reaction ± 0.5 Faint or mild erythema + One The border is clear but weak erythema, edema and palsy ++ 2 Clear erythema, papules and small follicles +++ 3 Severe erythema and alveoli, scab formation

Test substance After 1 hour After 24 hours Response (%)
(n = 20) ± + ++ ± + ++ Example 2 2 - - One - - 1.50 Comparative Example 1 3 One - 1.5 - - 1.80 Comparative Example 2 2 0.5 - 1.5 - - 1.70 Comparative Example 3 7 4 - 3 - - 8.50

As can be seen from the above Table 8, the cosmetic composition of Example 2, which is an emulsion type containing the yuza seed and mango seed oil of the present invention, has excellent skin irritation mitigation effect as compared with the cosmetic composition of Comparative Examples 1 to 3 And it was found.

Test Example  6: Stability test of formulation

The stability test of the cosmetic composition containing the yuza seed and the mango seed mixed oil of the present invention was carried out by the following method. To test the stability of the cosmetic, Example 2 and Comparative Examples 1 to 3 were placed in an opaque glass container in a thermostatic chamber maintained at 45 DEG C for a period of 12 weeks, and a completely shaded refrigerator And stored for 12 weeks. The degree of discoloration, separation, and separation were compared, and the results are shown in Table 9 below. At this time, the product discoloration, separation and degree of separation were classified into the following six grades and evaluated.

0: No change 1: Very little separation (discoloration, change)

2: little separation (discoloration, discoloration) 3: slight separation (discoloration, discoloration)

4: Severely separated (discolored, detached) 5: Severely separated (discolored, detached)

division Stability of formulation Example 2 Comparative Example 1 Comparative Example 2 Comparative Example 3 45 ° C 4 ℃ 45 ° C 4 ℃ 45 ° C 4 ℃ 45 ° C 4 ℃ discoloration 0 0 One 0 0 0 0 0 Fling 0 0 One 0 One 0 0 0 detach 0 0 0 0 0 0 0 0

As can be seen from the above Table 9, Example 2 shows that the mixture of yuza seed and mango seed oil was stable in the formulation at 45 ° C and 4 ° C with almost no discoloration, .

The cosmetic composition containing the citron seed oil and the mango seed oil mixture of the present invention can be produced by the following various formulations.

Example  3: Nourishing lotion ( Milk lotion )

Nutritional lotion containing yuza seed and mango seed mixed oil in the composition shown in the following Table 10 was prepared by a conventional method.

ingredient Content (% by weight) Citron seed oil and mango seed oil (Example 1) 1.0 glycerin 5.1 Propylene glycol 4.2 Tocopheryl acetate 3.0 Liquid paraffin 4.6 Triethanolamine 1.0 Squalane 3.1 Macadamia nut oil 2.5 Polysorbate 20 1.6 Sorbitan sesquiterate 1.6 Propylparaben 0.6 Carboxyl vinyl polymer 1.5 incense a very small amount antiseptic a very small amount Purified water Balance system 100

Example  4: Nourishing cream

Nutrition creams containing yuza seed and mango seed mixed oil in the composition shown in the following Table 11 were prepared by a conventional method.

ingredient Content (% by weight) Citron seed oil and mango seed oil (Example 1) 1.0 Pro-type glycerin monostearate 2.0 Stearyl alcohol 2.2 Stearic acid 1.8 Wax 1.0 Polysorbate 60 1.5 Sorbitan stearate 0.6 Purified vegetable oil 1.0 Squalane 3.0 Mineral oil 5.0 Trioctanoin 5.0 Dimethicone 1.0 Sodium magnesium silicate 0.1 glycerin 5.0 Betaine 3.0 Triethanolamine 0.8 Sodium hyaruronate 4.0 Preservative, fragrance, pigment a very small amount Purified water Balance Sum 100

Example  5: Massage  cream

A massage cream containing a yuza seed and a mango seed mixed oil in the composition shown in Table 12 below was prepared by a conventional method.

ingredient Content (% by weight) Citron seed oil and mango seed oil (Example 1) 1.0 glycerin 4.0 vaseline 3.5 Triethanolamine 0.2 Liquid paraffin 24.0 Squalane 3.0 Wax 2.1 Tocopheryl acetate 0.1 Polysorbate 60 2.4 Carboxyl vinyl polymer 0.2 Sorbitan sesquioleate 2.3 incense a very small amount antiseptic a very small amount Purified water Balance system 100

Example  6: Pack

A pack containing the yuza seed and the mango seed mixed oil in the composition shown in the following Table 13 was prepared by a conventional method.

ingredient Content (% by weight) Citron seed oil and mango seed oil (Example 1) 1.0 Ethyl alcohol 4.0 EDTA-2Na 0.02 Propylene glycol 5.1 glycerin 4.5 Carbopol 1.0 Polyoxide 0.3 antiseptic a very small amount incense a very small amount Purified water Balance system 100

Claims (6)

Citron seed oil and mango seed oil in an amount of 0.01 to 10% by weight based on the total weight of the cosmetic composition.  The cosmetic composition for skin irritation alleviation according to claim 1, wherein the mixed oil is a mixture of citron seed oil and mango seed oil at a weight ratio of 1:10 to 10: 1. The cosmetic composition for relieving skin irritation according to claim 1, wherein the cosmetic composition alleviates skin irritation caused by ultraviolet rays. The cosmetic composition for relieving skin irritation according to claim 1, wherein the cosmetic composition is to alleviate skin irritation caused by retinol. delete Citron seed oil and mango seed oil in an amount of 0.01 to 10% by weight based on the total weight of the cosmetic composition.