KR102632084B1 - Cosmetic composition for anti-oxidation, skin whitening, and improving of skin wrinkle containing Sanguisorba officinalis extract - Google Patents

Abstract

The present invention relates to a cosmetic composition for antioxidant, skin whitening, and skin wrinkle improvement containing cucumber grass extract as an active ingredient.
The cosmetic composition for antioxidant, skin whitening and skin wrinkle improvement according to the present invention is composed of natural substances, is safe for the skin, and has excellent skin whitening and skin wrinkle improvement effects. More specifically, the composition of the present invention effectively acts on skin whitening by inhibiting melanin synthesis and tyrosinase activity, effectively inhibits the expression of MMP-1, which directly acts on the creation of skin wrinkles, and increases the expression of collagen protein. It works effectively in preventing and improving skin wrinkles.

Description

Cosmetic composition for anti-oxidation, skin whitening, and improving of skin wrinkle containing Sanguisorba officinalis extract}

The present invention relates to a cosmetic composition for antioxidant, skin whitening and skin wrinkle improvement containing cucumber grass extract as an active ingredient.

Recently, interest in cosmetics derived from natural products is growing. In addition, as the demand for functional cosmetics increases, the problem of the skin's protective ability and regenerative ability being reduced due to various chemical ingredients used as functional ingredients is occurring. Accordingly, it is becoming popular to make and use cosmetics made from natural materials, ranging from toners and lotions to color cosmetics. In the case of people with skin diseases such as atopy or sensitive skin, interest in cosmetics derived from natural products that minimize skin irritation and have excellent skin improving activity is increasing.

The present inventors conducted research on natural materials that are non-toxic and have excellent antioxidant, skin whitening, and skin wrinkle improvement effects, and discovered that cucumber grass extract exhibits excellent antioxidant, skin whitening, and skin wrinkle improvement effects. In addition, while continuing research on the cucumber grass extract, when cucumber grass was used as a complex extract with mistletoe, vitamin tree fruit, Schisandra chinensis, tomato, and buckwheat, not only did the skin whitening and skin wrinkle improvement effects improve, but it also showed excellent antioxidant effects. The present invention was completed by discovering the fact that

KR 10-1020536 B KR 10-2133074 B

The problem to be solved by the present invention is to provide a cosmetic composition for antioxidant, skin whitening and skin wrinkle improvement containing cucumber grass extract as an active ingredient.

In order to achieve the above object, the present invention provides a cosmetic composition for antioxidant, skin whitening and skin wrinkle improvement containing cucumber grass extract as an active ingredient.

According to one embodiment of the present invention, the extract may be a hot water extract, an ultrasonic extract, or an extract of fermented lactic acid bacteria of the Lactobacillus genus.

According to one embodiment of the present invention, the extract may be an extract extracted after irradiating cucumber grass with ultraviolet UVB at 350 to 1000 mJ/cm ² .

According to one embodiment of the present invention, the composition may include the extract at a concentration of 5 to 200 μg/mL.

According to one embodiment of the present invention, the composition may further include mistletoe extract, vitamin tree fruit extract, Schisandra chinensis extract, tomato extract, and buckwheat extract.

According to one embodiment of the present invention, the composition contains cucumber grass extract, mistletoe extract, vitamin tree fruit extract, Schisandra chinensis extract, tomato extract, and buckwheat extract in the ratio of 1: 0.5-2.0: 0.5-2.0: 0.1-1.5: 0.05-1.0: It may be included at a weight ratio of 0.05-1.0.

According to one embodiment of the present invention, the composition may further include 5 to 15 parts by weight of turmeric extract based on the total weight of the extract.

According to one embodiment of the present invention, the cosmetic composition may be a formulation selected from lotions, emulsions, creams, essences, cosmetic ointments, sprays, gels, packs, sunscreens, makeup bases, foundations, powders, and makeup removers.

The cosmetic composition for antioxidant, skin whitening, and skin wrinkle improvement according to the present invention is composed of natural substances, is safe for the skin, and has excellent skin whitening and skin wrinkle improvement effects. More specifically, the composition of the present invention effectively acts on skin whitening by inhibiting melanin synthesis and tyrosinase activity, effectively inhibits the expression of MMP-1, which directly acts on the creation of skin wrinkles, and increases the expression of collagen protein. It works effectively in preventing and improving skin wrinkles.

Hereinafter, the present invention will be described in detail.

According to one embodiment of the present invention, the present invention provides a cosmetic composition for antioxidant, skin whitening and skin wrinkle improvement containing cucumber grass extract as an active ingredient.

The cucumber plant ( Sanguisorba officinalis L. ) is a perennial plant of the Rosaceae family of the dicotyledonous plant dicotyledon group, and is called cucumber plant because it emits a refreshing cucumber smell when the leaves are cut. The roots branched off from the thick rhizome become thick in the shape of a cylinder with sharp ends, and the stem grows straight up to about 1m, with branches branching at the top. The leaves are located alternately at each node and are feather-shaped compound leaves with an odd number of leaf segments. In general, leaves are elongated oval-shaped, have blunt ends, and have rough sawtooth edges. The flowers bloom from July to September, are dark red, and hang on water inflorescences. The flower spike is oval or inverted egg-shaped, and flowers begin to bloom from the top. The fruit is an achene, ripens in October, is square, and is surrounded by a calyx.

The cucumber plant extract may be an extract obtained by extracting one or more parts selected from the group including flowers, leaves, stems, fruits, seeds, roots, and outposts of the cucumber plant. Most preferably, the root of the cucumber plant is extracted, and the extract of the cucumber plant is The root can be referred to as Jiyu.

The cucumber grass extract may be extracted using a conventional method in the art, such as hot water extraction, immersion extraction, reflux cooling extraction, post-fermentation extraction, and ultrasonic extraction, but a method selected from hot water extraction, ultrasonic extraction, and post-fermentation extraction. Extraction using is preferable in terms of efficacy.

The cucumber grass is preferably extracted by mixing it with an extraction solvent at a weight ratio of 1:10 to 30, preferably 1:15 to 25, more preferably 1:18 to 22.

Specifically, the cucumber grass hot water extract can use hot water at 40 ℃ to 100 ℃ as an extraction solvent, preferably hot water at 65 ℃ to 95 ℃, more preferably hot water at 85 ℃ to 95 ℃. However, it is not limited to this. In addition, the extraction time is preferably 1 to 50 hours, more specifically 2 to 20 hours, and more specifically 2 to 10 hours, but is not limited thereto. If the weight ratio of the cucumber grass and the extraction solvent is outside the above range, the active ingredient of the cucumber grass may be extracted in a small amount in the extract.

In addition, the ultrasonic extract of cucumber grass may be extracted ultrasonically using water, C ₁ to C ₄ lower alcohol, or a mixture thereof as an extraction solvent. The lower alcohol may include 20 to 80% methanol, ethanol, butanol, or propanol. The extraction solvent for the ultrasonic extract is not particularly limited, but the ultrasonic extract extracted with a 60 to 80% ethanol aqueous solution is preferred for antioxidant, skin whitening, and skin wrinkle improvement.

In addition, the extract of the fermented cucumber grass may be a supernatant obtained after centrifuging the fermented product of cucumber grass, or an extract extracted after adding an extraction solvent to the fermented product of cucumber grass. However, in terms of efficacy, the supernatant obtained after centrifuging the fermented product of cucumber grass It is more desirable. The fermented cucumber grass product can be obtained by fermenting cucumber grass or cucumber grass extract with lactic acid bacteria, preferably by fermenting it with Lactobacillus rhamnosus HK-9 (Accession Number: KCCM11254P). Specifically, the cucumber grass fermentation product was inoculated with Lactobacillus rhamnosus HK-9 in an appropriate amount, for example, at a concentration of 1 × 10 ⁸ to 5 × 10 ⁹ cfu/mL, and then incubated at 25 to 45 ° C. for 36 to 60 hours. , preferably obtained by fermentation at 30 to 40°C for 40 to 55 hours.

The term "extract" used herein when referring to cucumber grass includes not only the crude extract obtained by treatment with an extraction solvent, but also processed products of cucumber grass extract. For example, cucumber plant extract can be prepared in powder form by additional processes such as reduced pressure distillation and freeze-drying or spray drying.

In a preferred embodiment of the present invention, the cucumber grass extract is obtained by irradiating cucumber grass with ultraviolet UVB at 350 to 1000 mJ/cm ² , preferably 600 to 950 mJ/cm ² , and more preferably 800 to 900 mJ/cm ² It may be an extract obtained after extraction. When extracting cucumber grass after irradiating it with ultraviolet UVB radiation in the above range, the skin whitening and skin wrinkle improvement effects of the cucumber grass extract are further improved. If the irradiation amount of ultraviolet UVB is less than the lower limit, the effect of ultraviolet irradiation becomes insignificant, and if it exceeds the upper limit, the skin whitening and skin wrinkle improvement efficacy of the cucumber grass extract may actually decrease.

As used herein, the term “including as an active ingredient” means containing an amount sufficient to achieve the efficacy or activity of the cucumber grass extract. The cosmetic composition of the present invention contains the cucumber extract at a concentration of 5 to 200 μg/mL, preferably 5 to 100 μg/mL, more preferably 5 to 50 μg/mL, and even more preferably 5 to 20 μg/mL. may be included. If the concentration of the cucumber grass extract of the present invention is less than the lower limit, the antioxidant, skin whitening, and skin wrinkle improvement effects may not be achieved to the desired extent, and if it exceeds the upper limit, the antioxidant, skin whitening, and skin wrinkle improvement effects increase as the concentration increases. It is undesirable because it may not increase the effectiveness or may be toxic. Meanwhile, as a result of in vitro experiments, when the concentration of the cucumber grass extract of the present invention was within the above range, significant effects were observed for antioxidant, skin whitening, and skin wrinkle improvement, but no side effects such as cytotoxicity were observed.

The cosmetic composition of the present invention contains cucumber grass extract as an active ingredient and can exert a whitening effect. More specifically, since it contains a whitening active ingredient derived from the cucumber grass extract as an active ingredient, it exhibits a significant tyrosinase activity inhibition effect, thereby inhibiting melanin biosynthesis. It may have an inhibitory effect.

In one embodiment of the present invention, the skin wrinkles may be caused by photoaging or natural aging, more preferably photoaging, and more preferably photoaging due to UV exposure, but are not limited thereto. The skin wrinkles are preferably based on the activity of MMP enzymes, but are not limited thereto. In general, the formation of skin wrinkles is promoted after UV exposure due to an increase in the expression and activity of MMP, an enzyme that decomposes collagen, a major component of the skin.

In addition, the cosmetic composition containing the cucumber grass extract as an active ingredient is characterized by increasing the expression of type I procollagen protein and inhibiting the expression of MMP-1 protein.

Specifically, the cosmetic composition containing the cucumber grass extract of the present invention inhibits the expression of matrix metallo-proteinase-1, which promotes collagen decomposition, while increasing the production of type I procollagen, thereby improving the skin Increased elasticity, skin regeneration, improvement of skin wrinkles, wound healing, repair and regeneration of damaged skin tissue; and may have effects such as preventing skin aging. In other words, it can have the effect of preventing or improving skin wrinkles.

The cucumber grass extract contained in the cosmetic composition for improving skin wrinkles of the present invention has the effect of removing skin wrinkles by itself, and even when mixed with substances known to be effective in improving skin wrinkles, The prevention or improvement effect can be maintained continuously.

In a preferred embodiment of the present invention, the cosmetic composition may contain as an active ingredient a mixed extract further comprising mistletoe extract, vitamin tree fruit extract, Schisandra chinensis extract, tomato extract, and buckwheat extract in addition to the cucumber grass extract.

In this specification, the term "mixed extract" refers to 1) a mixed form of cucumber extract, mistletoe extract, vitamin tree fruit extract, Schisandra chinensis extract, tomato extract, and buckwheat extract, or 2) cucumber grass, mistletoe, vitamin tree fruit, Schisandra chinensis, tomato, and buckwheat. It refers to a form in which the mixture is first mixed and then extracted. It is more preferable to prepare the mixed extract by preparing each extract separately and then mixing them as in 1) above in terms of antioxidant, skin whitening, and skin wrinkle improvement effects. Each of the above extracts may be extracted in the same manner as the cucumber grass extract.

The mixed extract of cucumber grass extract, mistletoe extract, vitamin tree fruit extract, Schisandra chinensis extract, tomato extract, and buckwheat extract of the present invention is a synergistic or collaborative effect between the extracts and/or the active ingredients in the extract. It was confirmed that it had more powerful skin whitening and skin wrinkle effects compared to the single extract.

In a preferred embodiment of the present invention, the cosmetic composition contains cucumber grass extract, mistletoe extract, vitamin tree fruit extract, Schisandra chinensis extract, tomato extract, and buckwheat extract in a ratio of 1:0.5-2.0:0.5-2.0:0.1-1.5:0.05-1.0. :0.05-1.0, preferably 1:0.5-1.5:0.5-1.5:0.1-1.0:0.1-0.5:0.1-0.5, more preferably 1:0.7-1.3:0.7-1.3:0.3- It may be included in a weight ratio of 0.7:0.1-0.4:0.1-0.4, more preferably 1:0.8-1.2:0.8-1.2:0.4-0.6:0.2-0.3:0.2-0.3. When the weight ratio of each extract is within the above range, the synergistic effect of the combined use of the cucumber grass extract, mistletoe extract, vitamin tree fruit extract, Schisandra chinensis extract, tomato extract, and buckwheat extract can be maximized.

In an exemplary embodiment, the cucumber extract, mistletoe extract, vitamin tree fruit extract, Schisandra chinensis extract, tomato extract, and buckwheat extract may be present in the composition in any amount desired to provide effective antioxidant, skin whitening, and skin wrinkle improvement effects. You can. According to one embodiment, the cucumber extract, mistletoe extract, vitamin tree fruit extract, Schisandra chinensis extract, tomato extract and buckwheat extract are used in an amount of 5 to 200 ㎍/mL, preferably 5 to 100 ㎍/mL, more preferably 5 to 50 ㎍/mL. It may be contained at a concentration of ㎍/mL, more preferably 5 to 20 ㎍/mL. If the content of the cucumber extract, mistletoe extract, vitamin tree fruit extract, Schisandra chinensis extract, tomato extract and buckwheat extract is less than the above range, it is difficult to expect the effect of the mixed extract, and if it exceeds the above range, the content increases. Not only is the increase in effect negligible, but there is a problem that the stability of the formulation is reduced. In other words, by including the mixed extract of the present invention in the above range, it is possible to realize excellent antioxidant, skin whitening and skin wrinkle improvement effects, and has formulation and product stability, and can also exhibit excellent effects at the optimal content in terms of economic efficiency.

The cosmetic composition of the present invention may further include 5 to 15 parts by weight of turmeric extract based on the total weight of the mixed extract. When the turmeric extract is included in the weight ratio relative to the total weight of the mixed extract, the antioxidant, skin whitening, and skin wrinkle improvement effects are further improved. If the content of the turmeric extract in the mixed extract is less than the lower limit, the effect due to the addition of the turmeric extract becomes insignificant, and if it exceeds the upper limit, the antioxidant, skin whitening, and skin wrinkle improvement effects increase due to the addition of the turmeric extract. There is a problem in that it actually decreases. The turmeric extract is not particularly limited, but may be prepared in the same manner as the cucumber grass extract.

According to a preferred embodiment of the present invention, the formulation of the cosmetic composition for antioxidant, skin whitening and skin wrinkle improvement includes lotion, emulsion, cream, essence, cosmetic ointment, spray, gel, pack, sunscreen, makeup base, foundation, powder and It may be selected from the group consisting of makeup removers.

According to a preferred embodiment of the present invention, the cosmetic composition for antioxidant, skin whitening, and skin wrinkle improvement may additionally include a dermatologically acceptable excipient in addition to the cucumber grass extract or a mixed extract containing cucumber grass extract.

The excipients are not limited thereto, but may include, for example, skin emollients, skin penetration enhancers, colorants, fragrances, emulsifiers, thickeners, and solvents. In addition, it may additionally contain fragrances, pigments, disinfectants, antioxidants, preservatives, and moisturizers, and may include thickeners, inorganic salts, synthetic polymer materials, etc. for the purpose of improving physical properties.

According to a preferred embodiment of the present invention, the moisturizing agent is glycerin, butylene glycol, propylene glycol, sorbitol, hexylene glycol, dipropylene glycol, diglycerin, 1,2-hexanediol, panthenol, betaine, squalane, petrolatum, liquid. It may be one or more selected from paraffin and hydrolyzed wheat gluten, but is not limited thereto.

The cosmetic composition of the present invention can be used alone or in multiple applications, or can be used in multiple applications with other cosmetic compositions other than the present invention. Additionally, the cosmetic composition according to the present invention can be used according to conventional usage methods, and the number of times of use can be varied depending on the user's skin condition or preference.

Hereinafter, the present invention will be described in detail through examples, but the present invention is not limited to the following examples.

<Example>

Example 1-1: Preparation of cucumber grass extract - ultrasonic extraction

Cucumber plant powder was prepared by pulverizing the roots (oil) of the cucumber plant.

Cucumber grass powder and 70% ethanol aqueous solution were mixed at a weight ratio of 1:20, and then extracted for 3 hours at 80°C with an ultrasonic extractor (Natural Solution; 120 kHz/30 min) to obtain an ethanol ultrasonic extract of cucumber grass. The obtained extract was filtered, concentrated under reduced pressure, and powdered by freeze-drying.

Example 1-2: Preparation of cucumber grass extract - ultrasonic extraction + ultraviolet irradiation

Cucumber grass extract was prepared in the same manner as in Example 1-1, except that the cucumber grass powder was irradiated with ultraviolet rays before extraction.

The ultraviolet irradiation is performed by placing the cucumber powder in a container (350*255*40 mm) for ultraviolet treatment and then applying ultraviolet UVB (300 nm) to the top of the container using an ultraviolet irradiation device (HD 2102.1, 2102.2, Delta OHM, Italy). Irradiation was conducted, and the container was turned over and mixed every time the irradiation amount exceeded 100 mJ/㎡. Afterwards, UV irradiation was stopped when the total UVB irradiation amount reached 900 mJ/cm ² .

Example 2-1: Preparation of cucumber grass extract - hot water extraction

Cucumber plant powder was prepared by pulverizing the roots (oil) of the cucumber plant.

Cucumber herb powder and purified water were mixed at a weight ratio of 1:20 and extracted at 90°C for 3 hours to obtain a cucumber herb hot water extract. The obtained extract was filtered, concentrated under reduced pressure, and powdered by freeze-drying.

Example 2-2: Preparation of cucumber grass extract - hot water extraction + ultraviolet irradiation

Cucumber grass extract was prepared in the same manner as in Example 2-1, except that the cucumber grass powder was irradiated with ultraviolet rays before extraction.

The ultraviolet irradiation was performed in the same manner as Example 1-2.

Example 3-1: Preparation of cucumber grass extract - fermentation extraction

Cucumber plant powder was prepared by pulverizing the roots (oil) of the cucumber plant.

Then, Lactobacillus rhamnosus HK-9 (accession number: KCCM11254P) obtained from the Korea Microorganism Conservation Center was placed in a shaking constant-temperature incubator with a medium prepared by adding 55% MRS medium and 0.5% L-Cysteine hydrochloride, and incubated for 50 minutes. Preculture was performed at ~60 rpm and 37°C for 48 hours.

To a 2L flask, 1L of lactic acid bacteria culture medium made from 55% MRS medium and 100g of cucumber grass powder were added, and the pre-cultured Lactobacillus rhamnosus HK-9 culture medium at a concentration of 3 × ¹⁰⁹ cells/ml was added to 5(v/v)%. After inoculation, it was fermented at 37°C for 48 hours.

The fermented product was centrifuged at 3,000 rpm for 10 minutes to obtain the supernatant, which was then filtered using 0.25 μM filter paper and used in the present invention.

Example 3-2: Preparation of cucumber grass extract - fermentation extraction + ultraviolet irradiation

Cucumber grass extract was prepared in the same manner as in Example 3-1, except that the cucumber grass powder was irradiated with ultraviolet rays before extraction.

The ultraviolet irradiation was performed in the same manner as Example 1-2.

Example 4-1: Preparation of mixed extract of cucumber grass - ultrasonic extraction

Carried out in the same manner as in Example 1-1, but prepared by adding mistletoe extract, vitamin tree fruit extract, Schisandra chinensis extract, tomato extract, and buckwheat extract, respectively, in the same manner as the cucumber herb extract, the cucumber herb extract, mistletoe extract, A mixed extract of cucumber grass was prepared by mixing vitamin tree fruit extract, Schisandra chinensis extract, tomato extract, and buckwheat extract at a weight ratio of 1:1:1:0.5:0.25:0.25.

Example 4-2: Preparation of mixed extract of cucumber grass - ultrasonic extraction + ultraviolet irradiation

Cucumber grass extract was prepared in the same manner as in Example 4-1, except that the cucumber grass powder was irradiated with ultraviolet rays before extraction.

The ultraviolet irradiation was performed in the same manner as Example 1-2.

Example 5-1: Preparation of mixed extract of cucumber grass - hot water extraction

Carried out in the same manner as in Example 1-1, but prepared by adding mistletoe extract, vitamin tree fruit extract, Schisandra chinensis extract, tomato extract, and buckwheat extract, respectively, in the same manner as the cucumber herb extract, the cucumber herb extract, mistletoe extract, A mixed extract of cucumber grass was prepared by mixing vitamin tree fruit extract, Schisandra chinensis extract, tomato extract, and buckwheat extract at a weight ratio of 1:1:1:0.5:0.25:0.25.

Example 5-2: Preparation of mixed extract of cucumber grass - hot water extraction

Cucumber grass extract was prepared in the same manner as in Example 5-1, except that the cucumber grass powder was irradiated with ultraviolet rays before extraction.

The ultraviolet irradiation was performed in the same manner as Example 1-2.

Example 6-1: Preparation of mixed extract of cucumber grass - fermentation extraction

Carried out in the same manner as in Example 1-1, but prepared by adding mistletoe extract, vitamin tree fruit extract, Schisandra chinensis extract, tomato extract, and buckwheat extract, respectively, in the same manner as the cucumber herb extract, the cucumber herb extract, mistletoe extract, A mixed extract of cucumber grass was prepared by mixing vitamin tree fruit extract, Schisandra chinensis extract, tomato extract, and buckwheat extract at a weight ratio of 1:1:1:0.5:0.25:0.25.

Example 6-2: Preparation of mixed extract of cucumber grass - fermentation extraction + ultraviolet irradiation

Cucumber grass extract was prepared in the same manner as in Example 6-1, except that the cucumber grass powder was irradiated with ultraviolet rays before extraction.

The ultraviolet irradiation was performed in the same manner as Example 1-2.

Example 7-1: Preparation of cucumber grass mixed extract (+turmeric) - ultrasonic extraction

Carried out in the same manner as in Example 4-1, but prepared by adding turmeric extract in the same manner as the cucumber extract, the cucumber extract, mistletoe extract, vitamin tree fruit extract, Schisandra chinensis extract, tomato extract, and buckwheat extract were mixed. A mixed extract of cucumber grass was prepared by mixing 10 parts by weight of turmeric extract with 100 parts by weight of the extract.

Example 7-2: Preparation of mixed extract of cucumber grass (+turmeric) - ultrasonic extraction + ultraviolet irradiation

Cucumber grass extract was prepared in the same manner as in Example 7-1, except that the cucumber grass powder was irradiated with ultraviolet rays before extraction.

The ultraviolet irradiation was performed in the same manner as Example 1-2.

Example 8-1: Preparation of cucumber grass mixed extract (+turmeric) - hot water extraction

Carried out in the same manner as in Example 5-1, but prepared by adding turmeric extract in the same manner as the cucumber extract, the cucumber extract, mistletoe extract, vitamin tree fruit extract, Schisandra chinensis extract, tomato extract, and buckwheat extract were mixed. A mixed extract of cucumber grass was prepared by mixing 10 parts by weight of turmeric extract with 100 parts by weight of the extract.

Example 8-2: Preparation of cucumber grass mixed extract (+turmeric) - hot water extraction + ultraviolet irradiation

Cucumber grass extract was prepared in the same manner as in Example 8-1, except that the cucumber grass powder was irradiated with ultraviolet rays before extraction.

The ultraviolet irradiation was performed in the same manner as Example 1-2.

Example 9-1: Preparation of cucumber grass mixed extract (+turmeric) - fermentation extraction

Carried out in the same manner as in Example 6-1, but prepared by adding turmeric extract in the same manner as the cucumber extract, then mixed with the cucumber extract, mistletoe extract, vitamin tree fruit extract, Schisandra chinensis extract, tomato extract, and buckwheat extract. A mixed extract of cucumber grass was prepared by mixing 10 parts by weight of turmeric extract with 100 parts by weight of the extract.

Example 9-2: Preparation of mixed extract of cucumber grass (+turmeric) - fermentation extraction + ultraviolet irradiation

Cucumber grass extract was prepared in the same manner as in Example 9-1, except that the cucumber grass powder was irradiated with ultraviolet rays before extraction.

The ultraviolet irradiation was performed in the same manner as Example 1-2.

< Test example>

statistical processing

All statistical analyzes were performed using SPSS version 18.0 (IBM, Chicago, IL, USA). Differences in mean values between experimental groups (p <0.05) were determined using one-way ANOVA with Duncan's post-hoc test. The following experiments were each repeated at least three times, and each experimental data was expressed as mean ± standard deviation (SD).

Test Example 1: Cytotoxicity evaluation using MTT assay

Cytotoxicity was measured using the MTT 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) reduction method.

Cultured HaCaT cells were mixed well in DMEM medium and adjusted to 1x10 ⁵ cells/mL, then 1 mL of prepared cells were added to a 24-well culture plate and cultured for 3-4 days. When the cells had grown to about 80%, they were treated with samples of various concentrations (50, 100, 200, and 500 ㎍/mL) in medium without fetal bovine serum and antibiotics, and then cultured for an additional 24 hours, and at the end of the culture. Cell viability was measured using the MTT reduction method.

Approximately one-tenth of the volume of the growth medium was added with MTT solution (5 mg/mL) to each well, and then cultured at 37°C for another 4 hours. Then, remove the medium, being careful not to let the formazan produced by reducing MTT go out into the medium. After leaving it at room temperature for 30 minutes to completely remove the remaining medium, dissolve the sample using DMSO, and measure the absorbance at 570 nm. was measured. Cell survival rate was calculated according to Equation 1 below and is shown in Table 1 below.

[Equation 1]

Cell viability (%) = (absorbance of sample treatment group / absorbance of control group) × 100

division Cell viability (%) Sample processing concentration (㎍/mL) 10 50 100 200 500 Control group (untreated group) 100 Example 1-1 113.1 132.3 116.5 99.7 94.9 Example 1-2 121.2 141.4 123.1 110.3 98.6 Example 2-1 108.6 124.5 110.2 101.2 95.4 Example 3-1 108.7 126.1 109.9 102.4 95.8 Example 4-1 115.5 136.4 112.7 103.4 96.3 Example 5-1 105.7 123.5 106.3 98.7 89.5 Example 6-1 102.1 120.7 108.4 101.6 93.4 Example 7-1 109.4 126.8 107.6 103.2 95.6 Example 8-1 102.6 122.1 101.7 97.6 91.2 Example 9-1 115.2 137.5 111.0 105.1 94.5

As shown in Table 1 above, it was confirmed that the samples according to the examples of the present invention can be safely used on human skin.

Test Example 2: Antioxidant activity

2-1: DPPH radical scavenging ability

After dissolving the test substance in distilled water, test solutions diluted to various concentrations were prepared.

100 μL of the test solution diluted to various concentrations and 100 μL of 0.15 mM DPPH (2,2-diphenyl-1-picrylhydrazyl) solution were added to a 96-well plate, mixed, and reacted at room temperature for 30 minutes, and then the absorbance was measured at a wavelength of 570 nm. . The results showed the removal activity of free radicals compared to the control group to which no sample was added, and ascorbic acid was used as the positive control group. DPPH radical scavenging ability was calculated using the formula below, and the SC ₅₀ value was calculated from the concentration at which the radical scavenging ability is 50% from the calibration curve for the radical scavenging ability at each concentration and is shown in Table 2 below. For reference, in the case of ascorbic acid, a positive control, the DPPH radical scavenging ability (%) was 15.8 ± 1.9%, 60.9 ± 1.9%, and 83.4 ± 0.8% at concentrations of 6.25, 12.5, and 25 ㎍/mL, respectively, and SC ₅₀ was 11.7 ± 0.4. It was ㎍/mL.

[Equation 2]

DPPH radical scavenging ability (%) = (1-A/B) x 100

(A: Absorbance of sample addition section, B: Absorbance of untreated section)

division DPPH radical scavenging ability (%) SC ₅₀
(㎍/mL) Sample processing concentration (㎍/mL) 7.8 15.6 31.2 62.5 125 Control group (untreated group) 100 Example 1 1-1 27.7±2.5 59.5±0.8 76.1±1.3 78.4±0.9 78.8±0.3 13.5±0.4 1-2 36.8±2.4 67.9±1.2 83.6±0.9 85.3±1.6 87.5±1.7 10.6±1.1 Example 2 2-1 22.8±1.0 55.4±1.7 69.1±0.2 77.5±1.1 79.9±0.7 15.1±0.4 2-2 30.2±1.3 61.6±1.5 74.3±1.1 80.6±1.2 83.2±2.6 14.3±1.2 Example 3 3-1 - - 6.9±0.6 31.9±1.3 65.3±0.9 92.4±3.6 3-2 9.2±2.3 14.6±0.9 21.6±1.4 45.3±2.1 77.5±1.3 65.2±2.7 Example 4 4-1 42.2±1.2 63.6±3.1 81.2±0.6 83.9±3.5 85.4±2.4 8.8±1.3 4-2 50.1±0.6 70.3±2.2 86.6±3.1 89.4±2.6 91.6±1.0 7.8±0.2 Example 5 5-1 37.3±3.6 58.9±0.6 75.8±2.3 81.7±2.9 86.1±1.2 14.8±2.2 5-2 45.2±1.4 64.3±3.5 80.7±1.9 84.1±0.8 90.4±1.3 8.6±0.3 Example 6 6-1 19.6±1.2 36.5±1.5 47.6±3.1 70.8±3.2 77.2±2.9 32.4±1.4 6-2 26.5±1.7 47.3±1.4 53.4±2.7 77.6±2.6 86.3±3.2 30.1±0.6 Example 7 7-1 48.2±3.6 69.8±4.0 86.3±2.4 89.6±1.2 92.7±2.3 8.0±0.7 7-2 53.6±1.5 74.3±2.8 89.1±0.3 91.4±1.9 93.6±1.1 7.5±3.2 Example 8 8-1 43.1±2.9 64.1±2.3 78.6±1.2 85.2±1.6 89.6±2.6 8.7±1.1 8-2 48.3±2.2 69.4±2.0 83.4±0.7 89.2±1.2 91.4±2.1 8.0±1.3 Example 9 9-1 25.4±0.6 44.8±0.8 61.7±2.2 75.3±1.8 81.1±2.0 16.9±2.3 9-2 31.9±1.7 50.7±0.3 67.2±1.8 80.2±0.6 85.4±1.5 15.5±2.1

As shown in Table 2, it can be confirmed that the extract according to the example shows excellent antioxidant effect.

Test Example 3: Whitening effect

3-1: Tyrosinase activity inhibition ability

The ability to inhibit tyrosine hydroxylase activity of tyrosinase, which hydroxylates tyrosine to 3,4-dihydroxyphenylalanine (DOPA), was evaluated. The test substances were dissolved in distilled water and diluted in 0.1 M phosphate buffer (pH 6.5) to prepare test solutions of various concentrations. In a 96-well plate, sequentially add 170 μL of 0.1 M phosphate buffer (pH 6.5), 10 μL of test solution diluted to various concentrations, 10 μL of 2000 unit/mL mushroom tyrosinase, and 10 μL of 1.5 mM L-tyrosine, then mix and cool at 37°C. After reacting for 15 minutes, absorbance was measured at a wavelength of 490 nm. The absorbance of the blank test solution was measured by reacting in the same manner by adding 0.1 M phosphate buffer (pH 6.5) instead of the test solution. The inhibition rate of tyrosinase enzyme activity was calculated using the formula below and is shown in Table 3 below.

[Equation 3]

Enzyme activity inhibition rate (%) = {(A-B)/A} x 100

(A: Absorbance after reaction of blank test solution, B: Absorbance after reaction of test solution)

division Tyrosinase activity inhibition rate (%) Sample processing concentration (㎍/mL) 5 10 15 20 Control group (untreated group) 100 Example 1 1-1 73.3±3.0 61.1±0.5 93.5±1.3 98.5±1.3 1-2 77.4±1.5 65.2±1.3 96.2±1.0 98.7±0.5 Example 2 2-1 62.9±4.2 61.6±1.2 87.6±0.6 97.7±1.0 2-2 67.5±2.6 66.2±0.7 90.4±1.1 98.3±1.2 Example 3 3-1 - - - 25.2±0.6 3-2 3.7±0.2 6.8±0.4 11.5±0.5 32.1±2.4 Example 4 4-1 79.5±2.3 72.2±1.5 96.3±2.7 99.6±0.5 4-2 83.6±1.2 75.4±0.8 97.5±1.9 99.5±0.1 Example 5 5-1 67.5±2.0 65.3±0.7 91.1±0.5 98.5±0.6 5-2 71.2±1.9 69.5±1.3 94.5±0.8 99.4±0.3 Example 6 6-1 14.3±1.3 19.5±2.2 27.7±2.6 45.6±1.9 6-2 18.5±1.9 25.6±0.9 33.4±1.2 52.8±2.1 Example 7 7-1 83.4±1.7 75.4±1.2 97.5±0.8 99.7±0.2 7-2 87.2±1.6 81.5±2.7 98.1±0.5 99.2±0.1 Example 8 8-1 71.6±1.0 69.5±2.1 93.4±0.7 98.8±0.2 8-2 74.5±1.1 72.7±0.8 95.1±0.6 99.1±0.4 Example 9 9-1 19.3±0.6 25.3±1.7 33.3±0.2 51.4±2.1 9-2 24.2±0.4 29.4±0.6 37.8±2.1 56.3±3.0

As shown in Table 3, the tyrosinase inhibitory effect of the extract according to the example was confirmed to be very excellent.

3-2: Melamine synthesis inhibition ability

B16F10 cell culture

B16F10 cells, melanoma cells derived from mice, were grown in Dulbecco's modified Eagle's medium (DMEM, Gibco-Invitrogen, Carlabad, CA, USA) with 10% fetal bovine serum (FBS), 100 units/mL penicillin, and 100 μg/mL streptomycin. The added cell culture medium was used to culture in a humid CO ₂ incubator (5% CO ₂ /95% air) at 37°C. When the culture dish was about 80% filled with cells, the cell monolayer was washed with phosphate buffer saline (PBS, pH 7.4), cell culture medium was added, cells were detached and subcultured, and the medium was changed every two days.

Measurement of melanin content produced in B16F10 cells

B16F10 cells were distributed in a 12-well plate at 5 × 10 ⁴ cells/well and cultured for 24 hours. After culturing the cells for 24 hours, the cell culture medium was exchanged with a culture medium containing 100 nM α-melanocyte-stimulating hormone (α-MSH, Sigma-Aldrich) and various concentrations of test substances. After culturing the cells for 72 hours, the cell monolayer was washed with PBS, treated with 0.25% trypsin-2.65 mM EDTA to recover the cells, and centrifuged at 12,000 rpm for 10 minutes to obtain a pellet. 1 N NaOH containing 10% dimethylsulfoxide (DMSO) was added to the obtained pellet to dissolve it at 60°C, and the absorbance was measured at a wavelength of 490 nm. The amount of melanin was calculated from a calibration curve using synthetic melanin, and the protein was measured using a BCA protein assay kit (Thermo Scientific). The amount of melanin produced was normalized to the amount of protein, and the value of the group treated only with α-MSH was set to 100. Expressed as a relative value. At this time, the positive control group is a test group treated with only 100 nM of α-MSH, and the comparison group is a test group treated with 10 ng/mL of TGF-β1. For reference, the melanin content of the untreated group in which neither α-MSH nor the sample was treated was measured to be 61.1 ± 8.8%.

division Melanin content (% of control) Sample processing concentration (㎍/mL) 5 10 20 50 Positive control group 100 TGF-β1 75.6±4.7 Example 1 1-1 74.9±2.4 82.7±6.2 80.2±1.5 56.0±2.7 1-2 71.3±1.5 77.6±2.1 74.1±0.7 53.2±1.6 Example 2 2-1 89.3±2.1 80.3±3.4 86.8±2.7 68.6±2.5 2-2 86.2±1.3 76.5±2.7 81.5±2.4 65.3±2.1 Example 3 3-1 86.5±2.5 80.0±2.9 80.0±2.4 88.4±8.1 3-2 83.1±1.2 77.1±0.8 75.6±0.3 86.1±1.6 Example 4 4-1 70.4±0.6 78.2±2.7 75.1±1.2 52.3±1.5 4-2 66.5±1.3 72.3±1.1 71.6±2.9 49.6±2.2 Example 5 5-1 85.2±2.6 77.3±2.0 83.1±2.6 62.3±3.1 5-2 81.9±2.1 72.1±1.3 78.2±1.5 61.4±1.3 Example 6 6-1 82.1±0.2 76.2±1.8 75.3±0.7 77.1±0.3 6-2 79.6±2.3 75.4±1.5 72.6±0.1 72.6±0.4 Example 7 7-1 68.1±1.2 74.3±1.4 71.6±0.9 50.1±1.0 7-2 67.2±0.6 71.6±0.8 67.4±1.8 46.5±2.6 Example 8 8-1 83.2±1.3 74.2±1.4 78.6±1.7 60.2±2.2 8-2 80.5±2.0 72.6±0.8 74.5±0.2 57.3±1.5 Example 9 9-1 78.0±0.6 73.5±0.4 73.7±0.9 78.4±2.1 9-2 77.4±0.4 71.3±1.2 69.6±0.7 73.1±1.6

As shown in Table 4, the melanin synthesis inhibition effect of the extract according to the example was confirmed to be very excellent.

Test Example 4: Skin wrinkle improvement effect

4-1: Promotes synthesis of type 1 procollagen protein

HS68 cell culture

HS68 cells, human-derived skin fibroblasts, were purchased from the Korea Cell Line Bank. HS68 cells were grown in DMEM medium (Welgene) using cell culture medium (complete DMEM medium) supplemented with 10% fetal bovine serum (FBS), 100 units/mL penicillin, and 100 μg/mL streptomycin, and incubated at 37°C in a humidified CO2 incubator (5 % CO2/95% air). When the culture dish was about 80% filled with cells, the cell monolayer was washed with phosphate buffer saline (PBS, pH 7.4), trypsin-2.65mM EDTA was added, cells were detached and subcultured, and the medium was changed every 2 days. .

Measurement of type 1 procollagen content produced and secreted by HS68 cells

HS68 cells were distributed in a 24-well plate at 1 × 10 ⁵ cells/well and the cells were cultured for 24 hours. After culturing the cells for 24 hours, they were replaced with serum-free cell culture medium containing various concentrations of each test substance, and the cells were cultured for 24 hours. After culturing the cells for 24 hours, the cell culture medium was collected and the procollagen type I C-peptide (PICP) content was measured according to the method suggested by the manufacturer using the Procollagen Type I C-Peptide (PIP) EIA Kit (Takara). was measured and shown in Table 5 below. At this time, the comparison group was a test group treated with 10 ng/mL of TGF-β1. For reference, the type 1 procollagen content of the untreated group was measured to be 280.0 ± 0.4 ng/mL.

division Type 1 procollagen content (ng/mL) Sample processing concentration (㎍/mL) 5 10 20 50 100 TGF-β1 497.0±6.0 Example 1 1-1 386.6±22.7 304.1±20.7 140.8±15.9 60.4±0.7 62.9±5.1 1-2 394±11.0 326.1±12.6 145.4±9.7 64.8±8.9 65.1±6.5 Example 2 2-1 279.5±4.6 273.7±7.9 189.5±7.3 92.5±10.4 52.9±5.8 2-2 283.7±13.8 279.3±3.4 195.4±6.7 95.8±9.1 57.5±1.9 Example 3 3-1 346.2±12.4 367.0±15.0 315.8±14.8 300.0±5.4 222.0±4.0 3-2 352.1±10.8 378.6±11.6 327.9±9.2 305.9±1.6 227.5±6.2 Example 4 4-1 423.6±2.3 349.2±10.4 186.4±11.5 105.9±10.1 112.6±4.7 4-2 430.8±11.2 362.5±11.0 203.7±13.6 126.1±12.7 139.5±6.3 Example 5 5-1 316.2±10.5 328.6±12.4 215.4±9.5 123.2±10.2 76.6±8.1 5-2 331.4±5.1 356.1±10.1 234.8±11.3 142.8±9.9 97.2±10.8 Example 6 6-1 364.8±13.5 396.4±11.0 352.8±16.1 338.1±7.6 264.1±7.3 6-2 381.6±15.4 415.2±6.1 376.2±10.4 352.8±9.2 281.6±6.6 Example 7 7-1 452.1±1.6 379.3±16.4 259.6±13.7 147.5±10.6 166.7±8.9 7-2 471.9±11.8 390.5±10.8 292.5±15.8 166.2±12.7 181.6±11.1 Example 8 8-1 332.1±12.0 341.1±11.9 231.8±14.6 153.8±10.1 96.4±10.5 8-2 351.6±10.4 356.0±10.6 254.6±9.7 172.9±6.4 116.2±5.7 Example 9 9-1 361.5±10.9 421.6±12.6 335.3±12.3 329.4±10.2 272.1±10.5 9-2 372.6±12.5 442.5±13.4 359.2±10.7 348.1±9.7 293.2±9.4

As shown in Table 5, it can be confirmed that the extract according to the example effectively prevents the formation of wrinkles and improves the formed wrinkles by promoting the production of type 1 procollagen.

4-2: Inhibition of synthesis of MMP-1 protein

HS68 cells were distributed in a 24-well plate at 1 × 10 ⁵ cells/well and the cells were cultured for 24 hours. After culturing the cells for 24 hours, they were replaced with serum-free cell culture medium containing various concentrations of each test substance, and the cells were cultured for 24 hours. After culturing the cells for 24 hours, the cell culture medium was collected and the MMP-1 content was measured using the Human MMP-1 ELISA Kit (Sigma-Aldrich) according to the method suggested by the manufacturer, and is shown in Table 6 below. For reference, the MMP-1 content of the untreated group was measured to be 4.21 ± 0.10 ng/mL.

division MMP-1 content (ng/mL) Sample processing concentration (㎍/mL) 5 10 20 50 100 TGF-β1 2.80±0.33 Example 1 1-1 3.56±0.19 2.47±0.22 2.17±0.12 2.01±0.24 1.81±0.09 1-2 3.46±0.06 2.41±0.18 2.09±0.21 1.99±0.08 1.80±0.04 Example 2 2-1 2.69±0.36 1.68±0.35 0.73±0.14 0.39±0.04 0.34±0.02 2-2 2.61±0.24 1.63±0.21 0.71±0.06 0.35±0.02 0.30±0.04 Example 3 3-1 3.10±0.22 2.14±0.07 0.96±0.11 0.54±0.07 0.34±0.02 3-2 2.98±0.16 2.10±0.10 0.92±0.05 0.49±0.02 0.31±0.06 Example 4 4-1 3.15±0.12 2.08±0.11 1.82±0.08 1.64±0.05 1.45±0.03 4-2 3.10±0.05 2.02±0.02 1.79±0.11 1.60±0.02 1.41±0.12 Example 5 5-1 2.50±0.21 1.47±0.29 0.59±0.13 0.28±0.03 0.25±0.04 5-2 2.46±0.15 1.42±0.12 0.54±0.04 0.25±0.05 0.21±0.03 Example 6 6-1 2.86±0.21 2.02±0.09 0.79±0.05 0.46±0.07 0.28±0.04 6-2 2.69±0.14 1.98±0.20 0.75±0.02 0.42±0.01 0.26±0.03 Example 7 7-1 3.02±0.09 1.96±0.15 1.70±0.04 1.53±0.07 1.38±0.02 7-2 2.97±0.10 1.92±0.03 1.66±0.10 1.50±0.09 1.34±0.07 Example 8 8-1 2.41±0.13 1.33±0.21 0.49±0.12 0.22±0.05 0.19±0.01 8-2 2.37±0.22 1.29±0.16 0.45±0.09 0.19±0.06 0.17±0.01 Example 9 9-1 2.71±0.19 1.89±0.13 0.71±0.08 0.39±0.06 0.22±0.02 9-2 2.67±0.11 1.84±0.08 0.67±0.12 0.35±0.03 0.19±0.01

As shown in Table 6, it can be confirmed that the extract according to the example effectively prevents the formation of wrinkles and improves the wrinkles formed through the action of suppressing MMP-1 expression.

Test Example 5: Human patch test

The human patch test was conducted in accordance with the ethical regulations based on the Declaration of Helsinki and the Ministry of Food and Drug Safety Cosmetics Human Application Testing Guidelines, using the method devised by Frosch & Kligman (J. Am. Acad. Dermatol, 1(1) 35 (1979) ) was applied to 32 healthy female subjects aged 20 to 60 (mean age 41.34 ± 8.06 years) without specific skin diseases or allergies. First, the patch area was disinfected with 70% ethanol, and then a Van der Bend (Van der Bend, Netherlands) patch containing 20 μl of the samples (200 μg/mL) of Examples 1 to 9 was applied. After 48 h, the patch was removed, the test site was marked with a skin marker (Chemotechnique Diagnostics AB, Sweden), and the reactivity of each test site was evaluated after 20 min and 24 hours. The evaluation criteria for the primary skin irritation test were in accordance with the PCPC Guidelines shown in Table 7 below, and the degree of skin reaction (average reactivity) evaluated twice was calculated according to Equation 4 below: Scored.

Responsiveness Clinical symptoms (reaction) 0 No response One faint or mild erythema 2 Moderate erythema; and barely detectable swelling and/or papule formation at the patch border. 3 Moderate erythema; and edema spread throughout the patch area. 4 severe erythema; and severe edema; or blisters 5 Severe reactions spread beyond the patch site

[Equation 4]

Average reactivity = [{(reactivity × number of subjects who responded)/(total number of subjects × highest score (5 points))} × 100] × 1/2

In order to evaluate the degree of skin irritation on human skin, a human patch test was performed by applying 20 μL of the samples of Examples 1 to 9 in liquid state to each of 32 subjects, and the results are shown in Table 8 below.

division Number of subjects who responded (people) Responsiveness 24 hours 48 hours average negative control 0 0.0 0.0 0.0 Example 1-1 0 0.0 0.0 0.0 Example 1-2 0 0.0 0.0 0.0 Example 2-1 0 0.0 0.0 0.0 Example 3-1 0 0.0 0.0 0.0 Example 4-1 0 0.0 0.0 0.0 Example 5-1 0 0.0 0.0 0.0 Example 6-1 0 0.0 0.0 0.0 Example 7-1 0 0.0 0.0 0.0 Example 7-2 0 0.0 0.0 0.0 Example 8-1 0 0.0 0.0 0.0 Example 8-2 0 0.0 0.0 0.0 Example 9-1 0 0.0 0.0 0.0 Example 9-2 0 0.0 0.0 0.0

Looking at Table 8, as a result of evaluating the degree of skin irritation by applying the sample according to the example of the present invention, all 32 subjects showed a negative reaction with a reaction grade of 0.0, so the composition of the present invention is a primary irritant to human skin. From this perspective, it is considered a non-irritating material.

The following illustrates a preparation example for the cucumber grass extract of the present invention.

<Manufacture example> Manufacture of cosmetics

Preparation Example 1: Soft lotion (skin)

To prepare an antioxidant, skin whitening, and skin wrinkle-improving softening lotion containing the cucumber grass extract of the present invention, it can be mixed as shown in Table 9 below and manufactured according to a conventional manufacturing method in the cosmetics field.

ingredient Content (% by weight) Cucumber grass extract of Example 1-2 50 μg/mL glycerin 6.0 1,3-butylene glycol 3.0 PEG 1500 1.0 allantoin 0.1 DL-panthenol 0.3 EDTA-2Na 0.02 Benzophenone-9 0.04 Sodium Hyaluronate 5.0 ethanol 10.0 Polysorbate 20 0.2 Preservatives, fragrance, coloring a very small amount Distilled water remaining amount Sum 100

Manufacturing Example 2: Nutritional lotion (lotion)

To prepare a nutritional lotion containing the cucumber grass extract of the present invention for antioxidant, skin whitening, and skin wrinkle improvement, it can be mixed as shown in Table 10 below and manufactured according to a conventional manufacturing method in the cosmetics field.

ingredient Content (% by weight) Cucumber grass extract of Example 1-2 50 μg/mL propylene glycol 6.0 glycerin 4.0 Triethanolamine 1.2 Tocopheryl Acetate 3.0 liquid paraffin 5.0 squalane 3.0 macadami nut oil 2.0 Polysorbate 60 1.5 Sorbitan sesquioleate 1.0 Carboxy vinyl polymer 1.0 Preservatives, fragrance, coloring a very small amount Distilled water remaining amount Sum 100

Manufacturing Example 3: Nutritional cream

To prepare a nutritional cream for antioxidant, skin whitening and skin wrinkle improvement containing the cucumber grass extract of the present invention, it can be mixed as shown in Table 11 below and manufactured according to a conventional manufacturing method in the cosmetics field.

ingredient Content (% by weight) Cucumber grass extract of Example 1-2 50 μg/mL Lipophilic glycerin monostearate 1.5 cetearyl alcohol 1.5 stearic acid 1.0 Polysorbate 60 1.5 Sorbitan Stearate 0.6 Isostearyl isostearate 5.0 squalane 5.0 mineral oil 35.0 Dimethicone 0.5 Hydroxyethylcellulose 0.12 glycerin 6.0 Triethanolamine 0.7 Preservatives, fragrance, coloring a very small amount Distilled water remaining amount Sum 100

Preparation Example 4: Emulsion for mask pack

To prepare a mask pack emulsion for antioxidant, skin whitening and skin wrinkle improvement containing the cucumber plant extract of the present invention, it can be mixed as shown in Table 12 below and prepared according to a conventional manufacturing method in the cosmetics field.

ingredient Content (% by weight) Cucumber grass extract of Example 1-2 50 ㎍/mL polyvinyl alcohol 15.0 cellulose gum 0.15 glycerin 3.0 PEG 1500 2.0 Cyclodextrin 0.15 DL-Panthenol 0.4 allantoin 0.1 Monoammonium glycyrrhizinate 0.3 nicotinamide 0.5 ethanol 6.0 PEG 40 Hydrogenated Castor Oil 0.3 Preservatives, fragrance, coloring a very small amount Distilled water remaining amount Sum 100

Although the present invention has been described in terms of the above-mentioned preferred embodiments, various modifications and variations can be made without departing from the gist and scope of the invention. Additionally, the appended claims cover such modifications or variations as fall within the subject matter of the present invention.

Claims (7)

Contains cucumber plant extract as an active ingredient,
A cosmetic composition for antioxidant, skin whitening and skin wrinkle improvement, characterized in that the extract is an extract extracted after irradiating cucumber grass with ultraviolet UVB at 350 to 1000 mJ/cm ² . According to paragraph 1,
A cosmetic composition, characterized in that the extract is a hot water extract, an ultrasonic extract, or an extract of fermented lactic acid bacteria of the Lactobacillus genus. delete According to paragraph 1,
A cosmetic composition comprising the extract at a concentration of 5 to 200 μg/mL. According to paragraph 1,
The cosmetic composition further comprises mistletoe extract, vitamin tree fruit extract, Schisandra chinensis extract, tomato extract, and buckwheat extract. According to clause 5,
The composition contains cucumber extract, mistletoe extract, vitamin tree fruit extract, Schisandra chinensis extract, tomato extract and buckwheat extract in a weight ratio of 1: 0.5-2.0: 0.5-2.0: 0.1-1.5: 0.05-1.0: 0.05-1.0. Characterized cosmetic composition. According to clause 6,
The cosmetic composition further comprises 5 to 15 parts by weight of turmeric extract based on the total weight of the extract.