KR101515443B1 - The functional cosmetic composition for anti-wrinkle, skin whitening and recovery of distrupted skin containing, comprising the mixture of extract of bergamot, Lonicera japonica Thunberg, Coix lachryma-jobi var. mayuen and wheat bud - Google Patents

Abstract

The present invention relates to a cosmetic composition for wrinkle improvement and whitening comprising bergamot extract, gingerolite extract, yulmu extract and wheat extract as an active ingredient, wherein the cosmetic composition for wrinkle improvement according to the present invention comprises And it can be effectively used as a whitening cosmetic composition. In addition, since the effect of accelerating recovery of skin tissue damage caused by a chemical substance is also excellent, it is possible to provide a cosmetic composition for recovering damaged tissue having excellent damage. In addition, when the mixed extract is mixed with a mangosteen extract, a liquorice extract, a marigold extract, a windbreak extract, a myrtle licorice extract, and a biradja extract, it is more effective. Since the composition exhibits various functionalities, it can be utilized as a multi-functional composition.

Description

TECHNICAL FIELD The present invention relates to a functional cosmetic composition for improving wrinkles, whitening and damaged skin, which comprises bergamot, europaea, yulmu, and germ extract, and which comprises a functional cosmetic composition for anti-wrinkle, bergamot, Lonicera japonica Thunberg, Coix lachryma-jobi var. mayuen and wheat bud}

The present invention relates to a cosmetic composition for improving wrinkles and whitening, and more particularly, to a cosmetic composition for improving wrinkles and whitening, which contains a mixture of bergamot, gingerbread, yulmu and wheat extracts having wrinkle improving effect, whitening activity effect, To a cosmetic composition.

Skin is the primary barrier of the human body. It protects the internal organs of the body from external stimuli such as temperature and humidity changes, ultraviolet rays, and pollutants, and plays an important role in keeping the body homeostatic such as body temperature control. This important skin also becomes aged gradually as the age of the human body grows, and as a result, skin elasticity loss, keratinization, wrinkle formation, skin atrophy and the like appear. This aging of the skin not only causes wrinkles on the skin surface but also changes the entire line of the face. The appearance of wrinkles on the skin is a phenomenon accompanied by aging, which shows various structural changes due to the deterioration of vital function. That is, it is divided into intrinsic aging and aging caused by aging, and photoaging due to the addition of external factors, especially ultraviolet damage.

Much research has been carried out on the importance and role of collagen directly affecting such wrinkles and skin elasticity. When the majority of the skin, 77% of the skin, and 90% of the dermis are rich in quantity and quality of collagen, the skin can also be healthy and beautiful without wrinkles and sagging. Conventionally, collagen has been commercialized in cosmetics. However, when collagen is applied to the skin surface as a cosmetic, it is difficult to absorb the transdermal absorption of collagen, which is a polymer, so that the function thereof can not be sufficiently expected. In addition, there was a method of injecting collagen directly into the dermis of the skin, but this method has not been a solution for improving the wrinkles of the skin due to side effects. In recent years, retinoic acid and proteins derived from animal placenta (Japanese Patent Laid-Open Publication No. 8-231370) have been known as substances promoting collagen synthesis. However, since retinoic acid is unstable, its formulation technique is complicated, and it is limited in terms of stability such as irritation to the skin. Animal placenta-derived protein has a fatal disadvantage that it is possible to use an extract of cattle suffering from mad cow disease.

On the other hand, as a pigment present in the epidermis of the skin, melanin is made from melanocytes existing in the basal layer of the epidermis and transferred to epidermal cells called keratinocytes. In vivo, there is no enzyme that degrades melanin, but it is removed in such a way that keratinocytes fall off the skin as they fall off the epidermis. Melanin has various functions in vivo. For example, melanin absorbs the light energy of sunlight UV and protects skin organs below the dermis from damage by UV. It also acts to remove harmful oxygen and free radicals generated in the skin in vivo, And protects the skin from inflammation. In addition, the amount of melanin in the skin cells determines the skin color of a person. A person with a lot of melanin pigment has a brown or black skin while a person with a little melanin pigment has a white skin.

Depending on the amount of melanin produced, it can cause various diseases. When melanin is abnormally low in production, skin lesions such as vitiligo are induced. On the other hand, when melanin is accumulated too much due to excessive synthesis of melanin due to sunlight, hormonal changes, inflammation, medicines or the like and melanin accumulation is not performed smoothly, it not only damages the skin but also deposits skin pigment and freckles , And these lesions lead to skin cancer. Therefore, in order to prevent such pigment deposition phenomenon, it is necessary to inhibit a part of the melanin production process to reduce the production of melanin.

Today, oriental women prefer white, clean skin and use it as an important criterion of beauty, so development of whitening agents to treat skin pigmentation abnormalities and to meet beauty needs is actively being carried out. Currently, in the development of a whitening agent for skin cosmetics, there is known a method of decolorizing by reducing the produced melanin pigment and a method of inhibiting the activity of tyrosinase, an enzyme that forms a melanin pigment. As a whitening ingredient in the conventional cosmetics field, for example, substances inhibiting tyrosinase enzyme activity such as kojic acid and arbutin, hydroquinone, vitamin C and derivatives thereof, various plant extracts Has been used. However, the use of such whitening ingredients is limited due to poor stability in the preparation, coloration by decomposition, generation of offensive odor, efficacy at a biological level, unclear effect and stability, and the like.

On the other hand, bergamot is an evergreen shrub with dicotyledonous mulberry tree. Bergamot essential oil is a colorless liquid with a fresh and tangy flavor that is a citrus oil that stabilizes the mind, helps in breathing, and relieves tense muscles from mental stress. Gingkohwa refers to the flower bud of Lonicera japonica Thunberg or its varieties. It is used when fever is reduced, chest tightness and thirst are present, and it is known to have antibacterial, anti-inflammatory and antipyretic effects. In addition, Coix lachryma-jobi var. Mayuen is a one-year-old herbaceous plant belonging to the genus Picea. In particular, diuretic, analgesic, epidermal, and tonic effects are known to be used as medicines for edema, neuralgia, rheumatism, and bladder stones. In addition, it is said that the buds of the buds and the monocotyledonous plants, such as wheat, wheat, barley and other cereal sprouts, have the same nutrients as the vegetable before the buds are formed, and the buds and components are completely different. It is also known that wheat contains various vitamins, 82 minerals, 30 yeasts and 17 amino acids.

In addition, it is said to be the outpost of Portulaca oleracea L., and is known to have fever, detoxification and antibacterial activity. Other names include Maji, Five Seasons, Shoji Lion, Machi, Machi, and Sanshin. In addition, the term "dried" refers to a dried medicament obtained by removing roots or junks of Atractylodes japonica Koidzumi or Atractylodes macrocephala Koidzumi. It is pharmacologically known to promote cell immune function, anti-ulcer, liver function and immune function. It is also known as Dong-Paek, Daegeum, Pater, Cheon-gang, Dae-jung, Daejung and so on. It is also called Inula britannica var. Chinensis, which means perennial plant of the dicotyledonous plant. Dried flowers are known to have the efficacy of germane, jinhae, rhizome, gilt, and soothing

Also, Eclipta prostrate is an annual plant of Asteraceae with a height of 10 ~ 70 cm. It is a plant that occurs frequently in bean fields in Jeju-do, southern part, and central part of our country. It has been known to be effective in promoting blood flow, pain, immunity, dermatitis, promotion of hair growth, In addition, Paeonia japonica is a perennial herbaceous herbaceous plant belonging to the family Ranunculaceae, which is known to use roots for analgesia, gynecomastia, and women's diseases. In addition, Lepidoptera (Ledebouriella seseloides) is a perennial herbaceous herbaceous plant, which is grown in the northeastern part of China, Habei, and Inner Mongolia, as a medicinal crop. In one room, it is known that the roots of the two-year-olds are used as medicines for colds and headaches, sweating and gossip.

Accordingly, the present inventors have made efforts to develop a whitening composition having excellent stability and excellent wrinkle-reducing effect and inhibiting melanin formation and having an excellent whitening effect. As a result, it has been found that the combination of bergamot extract, giitta extract, yulmu extract, The wrinkle improving effect and the whitening effect are remarkably increased. In addition, it was confirmed that the above-mentioned mixed extract can be effectively used for skin damage, thus completing a multi-functional cosmetic composition.

Korean Patent Publication No. 10-2009-0091547 Summary and claims

It is an object of the present invention to provide a multi-functional cosmetic composition having a wrinkle-improving effect, a whitening effect and a damaged skin recovery effect, which contain bergamot, gingkojum, yulmu and wheat as active ingredients.

In order to accomplish the above object, one embodiment of the present invention provides a multi-functional cosmetic composition for improving wrinkles and whitening comprising bergamot extract, ginger europaea extract, yulmu extract, and wheat extract.

The extract may be prepared using conventional solvents known in the art, that is, under ordinary temperature and pressure conditions, using conventional solvents. Examples of the extraction solvent that can be used in the production of the bergamot, gingkojum, yulmu and wheat extract of the present invention include water, lower alcohol having ₁ to ₃ carbon atoms, acetone, ethyl acetate, butyl acetate and 1,3 butylene glycol And the like can be used alone or in combination. The suitable amount of the extraction solvent may be about 1 to 15 times, 5 to 12 times, and 10 times, but not limited to, about 1 to 15 times the weight of bergamot, gingerbread, yulmu or wheat germ. As the extraction method, hot water extraction, cold extraction, reflux cooling extraction and ultrasonic extraction can be used, and they can be extracted once or repeatedly. Further, the extraction temperature is not particularly limited as long as the effective activity of bergamot, gingkojum, yulmu, or bloom useful components is not removed, but is preferably extracted by immersion at room temperature.

The bergamot, gonghwaseum, yulmu, and ginseng extracts alone are low in collagen production and collagenase inhibitory effect, but they can be used as cosmetics for improving wrinkles when they are mixed and used. Further, the mixing ratio and the content can be appropriately changed by those skilled in the art in order to exhibit the optimum effect.

The bergamot extract, ginger extract, ganoderma lucidum extract and wheat germ extract may be contained at a weight ratio of 1 to 10: 1 to 10: 1 to 10: 1 to 10, and 2 to 8: 2 to 8: 2 to 8: 8 < / RTI > by weight. However, the content ratios can be mixed at various weight ratios depending on the characteristics of the product.

In addition, the mixed extract has a whitening effect as well as a wrinkle-reducing effect.

As used herein, the term "whitening" is understood as a result of inhibition of the production of melanin, specifically as a result of prevention or suppression of symptoms resulting from an increase in melanin as the production of melanin is inhibited. Examples of such symptoms include spots, freckles, skin aging, and the like. However, as shown in the following Examples and Experimental Examples of the present invention, the extract contained in the skin whitening composition of the present invention inhibits the production of melanin As long as the effect is evident, the symptoms should be understood as all the symptoms leading to skin whitening by preventing or inhibiting symptoms resulting from an increase in melanin.

The composition for skin whitening of the present invention may contain a compound or a plant extract already known to have a skin whitening effect so as to enhance or reinforce the skin whitening effect, in addition to the active ingredients, such as bergamot extract, gingkoff extract, yulmu extract and wheat extract , And other known compounds or plant extracts known to have a skin whitening effect, and may further include compounds or plant extracts having a skin whitening effect to be revealed in the future.

Examples of the compounds and plant extracts known to have the above-mentioned skin whitening effect include compounds such as mercaptosuccinic acid, mercaptodextran, teprenone, dihydroxy-isoquinoline, indomethacin, 3-hydroxymannu, vitamin K, thiazolidone But are not limited to, chinorenin, lemon extract, cucumber extract, rhododendron extract, licorice extract, rosemary extract, acerola cherry extract, bank extract, carob extract and geranium extract.

The above-mentioned bergamot extract, gingkohwase extract, yulmu extract, and wheat extract were found to have tyrosinase inhibitory activity and melanin synthesis inhibitory effect similar to arbutin, which is well known as a cosmetic composition for whitening. In particular, the inhibitory activity of tyrosinase inhibition was significantly increased in the treatment of mixed extracts of bergamot, ganghwase, yulmu, and wheat germ, respectively, compared to the treatment of bergamot extract, giitta extract, yulmu extract or ginseng extract.

In addition, one specific example of the present invention provides a cosmetic composition for improving wrinkles and whitening comprising, as an active ingredient, bergamot extract, giitta extract, yulmu extract, moss extract, horsetail extract, chrysanthemum extract and perilla extract.

The mashin extract, mash extract, and mushroom extract can also be extracted by the same extraction method as that of obtaining an extract of bergamot, eucalyptus, yulmu, or wheat.

The combined extracts of bergamot extract, giitta extract, yulmu extract and wheat extract alone have collagen production and collagenase inhibitory effect and can be used alone as a cosmetic product for wrinkle improvement. However, it is preferable that the mashin-like extract, the chrysanthemum extract, and the wild chrysanthemum extract are mixed together because they have a better collagen-producing effect and collagenase-inhibiting effect.

In addition, it was confirmed that the above-mentioned bergamot extract, gingkohwase extract, yulmu extract, ginseng extract, horseradish extract, chrysanthemum extract and perilla extract had similar tyrosinase inhibitory effect and melanin synthesis inhibitory effect compared to arbutin. In particular, the inhibitory activity of tyrosinase inhibition was significantly increased in the treatment of the mixed extracts of bergamot, gingkojum extract, yulmu extract, ginseng extract, mackinel extract, chrysanthemum extract, and wild ginseng extract.

Wherein said bergamot extract, gingerbread extract, yulmu extract, wheat germ extract, horsetail extract, chrysanthemum extract and geranium extract are mixed at a ratio of 1 to 10: 1 to 10: 1 to 10: 1 to 10: 1 to 10: 1 to 10: And it is preferably contained in a weight ratio of 2 to 8: 2 to 8: 2 to 8: 2 to 8: 2 to 8: 2 to 8: 2 to 8. However, the content ratios can be mixed at various weight ratios depending on the characteristics of the product.

In addition, one specific example of the present invention is a pharmaceutical composition comprising any one selected from the group consisting of bergamot extract, gingerbread extract, yulmu extract, mushroom extract, mashin extract, chrysanthemum extract, The present invention also provides a cosmetic composition for improving wrinkles and whitening comprising a mixture containing

The above-mentioned windshield extract, hanchikcho extract, and vinegar extract can also be extracted by the same extraction method as that for obtaining the above-mentioned bergamot, ganghwase, yulmu, and wheat extracts. The combined extracts of bergamot, gonghwaseum, yulmu, ginseng, mackinaceae, chrysanthemum, and chrysanthemum can be used as cosmetics for improving wrinkles by themselves because they have a collagen production effect and a collagenase inhibitory effect by themselves. However, it is preferable to mix windshield extract, hanchikcho extract or vile extract, since they have better collagen production and collagenase inhibiting effect.

Wherein one of bergamot extract, gingerbread extract, yulmu extract, wheat extract, mashin extract, chrysanthemum extract and wild mulberry extract, windshield extract, myrrhicin extract, and albedo extract is mixed at a ratio of 1 to 10: 1 to 10: 1 to 10: 2 to 8: 2 to 8: 2 to 8: 2 to 8: 2 to 10: 1 to 10: 1 to 10: 8: 2 to 8 by weight. However, the content ratios can be mixed at various weight ratios depending on the characteristics of the product.

In another embodiment of the present invention, there is provided a cosmetic composition for accelerating damaged skin recovery comprising bergamot extract, gingerol extract, yulmu extract and wheat extract as active ingredients.

The above extract can be extracted in the same manner as described above. Wherein the cosmetic composition contains bergamot extract, ginger extract, yulmu extract and wheat extract at a weight ratio of 1: 10: 1 to 10: 1 to 10: 1 to 10, 2: 8: 2 to 8: : 2 to 8 by weight or 4 to 6: 4 to 6: 4 to 6: 4 to 6 by weight. However, the content ratios can be mixed at various weight ratios depending on the characteristics of the product. The mixed extracts of bergamot, giunghwa, yulmu and germ were excellent in the proliferative effect of fibroblast and the effect of promoting recovery of skin tissue damage caused by chemical substances.

In addition, one specific example of the present invention provides a cosmetic composition for promoting damaged skin recovery comprising bergamot extract, gingkojum extract, yulmu extract, moss extract, mashin extract, extract of Lycoris spp.

The cosmetic composition may contain 1 to 10: 1 to 10: 1 to 10: 1 to 10: 1 to 10: 1 to 10: 1 of bergamot, gingerbread, yulmu, shirou, 1 to 10, or 2 to 8: 2 to 8: 2 to 8: 2 to 8: 2 to 8: 2 to 8: 2 to 8, 6: 4 to 6: 4 to 6: 4 to 6: 4 to 6: 4 to 6. However, the content ratios can be mixed at various weight ratios depending on the characteristics of the product.

It was confirmed that the proliferation effect of Fibroblast was significantly superior to that of the single extracts of the bergamot extract, Gingkohwabu extract, Yulmu extract, Mushroom extract, Matsuchi extract, Lycoris spp. In addition, it was confirmed that the effect of accelerating the recovery of skin tissue damage by chemicals was also superior to that of each extract.

In addition, one specific example of the present invention further includes any one selected from the group consisting of bergamot extract, gingerbread extract, yulmu extract, wheat extract, mashin extract, chrysanthemum extract, A cosmetic composition for accelerating the recovery of damaged skin comprising a mixed extract as an active ingredient.

Wherein one of bergamot extract, gingerbread extract, yulmu extract, wheat extract, mashin extract, chrysanthemum extract, wild mugwort extract, windshield extract, myrrhicin extract, and albedo extract is mixed at a ratio of 1 to 10: 1 to 10: 1 to 10: 2 to 8: 2 to 8: 2 to 8: 2 to 8: 2 to 10: 1 to 10: 1 to 10: 8: 2 to 8 by weight. However, the content ratios can be mixed at various weight ratios depending on the characteristics of the product.

The mixed extracts showed that the proliferation effect of fibroblasts on cells was remarkably superior to that of single extracts. In addition, it was confirmed that the effect of accelerating the recovery of skin tissue damage caused by chemicals was also superior to that of each extract.

The mixed extract according to one embodiment of the present invention may be contained in the product in an amount of 1 to 30% by weight or in an amount of 5 to 20% by weight. The cosmetic may be any one selected from the group consisting of softening longevity, nutrition lotion, nutritional essence, nutritive cream and pack, but can be used in various forms as long as it is used for wrinkle remover, skin whitening or damaged skin recovery .

In addition, one specific example of the present invention is a mixed extract of bergamot extract, gingerbread extract, yulmu extract and wheat extract, bergamot extract, gingerbread extract, yulmu extract, moss extract, mash extract, The present invention provides cosmetics containing a mixed extract of an extract, a gold europium extract, a yulmu extract, a wheat extract, a marigold extract, a chrysanthemum extract, a wild chrysanthemum extract, The mixed extract may be contained in an amount of 1 to 30% by weight, and may be contained in an amount of 5 to 20% by weight.

In addition, the cosmetic composition containing the extract of the present invention may contain, in addition to the above extract, conventional supplements and carriers such as antioxidants, stabilizers, solubilizers, vitamins, pigments, and perfumes, have. The cosmetic composition of the present invention may be prepared in any form conventionally produced in the art and may be in the form of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant- But are not limited to, cleansing, oil, powder foundation, emulsion foundation, wax foundation, and spray. More specifically, it can be manufactured in the form of a flexible lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a hair tonic, a shampoo, a rinse, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray and a powder .

When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butylglycol, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

When the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component is selected from aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters.

The mixed extracts of bergamot extract, giitta extract, yulmu extract and germ extract of the present invention have excellent wrinkle-improving effect and whitening effect and can be utilized as a complex functional cosmetic composition. In addition, the effect of accelerating recovery of skin tissue damage caused by chemicals is also excellent. Furthermore, when the mixed extract is mixed with a mangosteen extract, an extract of Lycoris sieboldii, a manganese extract, a windshield extract, a myrtle licorice extract, and a vinegar extract, it is more excellent and can be utilized as a complex functional composition.

FIG. 1 shows the amount of collagen biosynthesis according to the treatment of the mixed extract prepared by various mixing methods.
FIG. 2 shows the inhibition of collagenase activity by treatment of mixed extracts prepared by various mixing methods.
FIG. 3 shows the degree of melanin production according to the treatment of mixed extracts prepared by various mixing methods.
FIG. 4 shows the effect of whitening on the treatment of mixed extracts prepared by various mixing methods.
FIG. 5 shows the degree of cell proliferation according to the treatment of the mixed extract prepared by various mixing methods.
FIG. 6 shows the degree of TEWL according to the treatment of mixed extracts prepared by various mixing methods.
Figure 7 shows cell viability in the treatment of mixed extracts prepared by various mixing methods.

The present invention will be described in more detail with reference to the following Examples and Test Examples, but the present invention is not limited to the following Production Examples, Examples and Experimental Examples.

Manufacturing example . Preparation of mixed extracts

Production Example 1. Preparation of bergamot extract

The dried bergamot leaves were ground and 500 g of crushed bergham leaves were added to 5 l of tertiary distilled water and heated at 75 캜 for 24 hours. This was filtered with a paper filter, Watman # 2, to remove solids. The filtrate was lyophilized using a vacuum distillation apparatus (EYELA, JP / N-1000) to obtain 42 g of bergamot extract. The extracts were sterilized by irradiation with 60Co gamma rays (10 kGy / H, 25 kGy).

Manufacturing example  2. Manufacture of Euglena extract

The dried gold silver was pulverized and 500 g of ground gold silver was added to 5 liters of tertiary distilled water and heated at 75 占 폚 for 24 hours. The filtrate was concentrated by using a vacuum distillation apparatus (EYELA, JP / N-1000) and then lyophilized to obtain 45 g of a gingko biloba extract. The extracts were sterilized by irradiation with 60Co gamma rays (10 kGy / H, 25 kGy).

Manufacturing example 3. Preparation of yulmu extract

The dried yulmu was pulverized, and 5 l of tertiary distilled water was added to 1 kg of pulverized yulmu powder and extracted three times at 90 ° C to 95 ° C for 3 hours. The filtrate was concentrated by using a vacuum distillation apparatus (EYELA, JP / N-1000) and then lyophilized to obtain 118 g of a Yulmu extract. The extracts were sterilized by irradiation with 60Co gamma rays (10 kGy / H, 25 kGy).

Manufacturing example  4. Manufacture of extracts

To 500 g of dried wheat germ, 5 L of distilled water was added and the mixture was heated at 75 캜 for 24 hours. The filtrate was filtered through a paper filter, Watman # 2, to remove the solids. The filtrate was concentrated using a vacuum distillation apparatus (EYELA, JP / N-1000) and lyophilized to obtain 38 g of the extract. The extracts were sterilized by irradiation with 60Co gamma rays (10 kGy / H, 25 kGy).

Manufacturing example  5. Machi  Preparation of extract

To 500 g of dried seaweed, 5 L of tertiary distilled water was added and the mixture was heated in a hot water bath at 75 캜 for 24 hours. The filtrate was concentrated by using a vacuum distillation apparatus (EYELA, JP / N-1000) and lyophilized to obtain 45 g of a crude extract. The extracts were sterilized by irradiation with 60Co gamma rays (10 kGy / H, 25 kGy).

Manufacturing example  6. Preparation of Lycopersicon esculentum Extract

To 500 g of dried liquor, 5 L of tertiary distilled water was added and the mixture was heated in a hot water bath at 75 캜 for 24 hours. The filtrate was concentrated by using a vacuum distillation apparatus (EYELA, JP / N-1000) and lyophilized to obtain 41 g of an extract. The extracts were sterilized by irradiation with 60Co gamma rays (10 kGy / H, 25 kGy).

Manufacturing example  7. Manufacture of Dried Fruits

5 g of distilled water was added to 500 g of dried persimmon and heated at 75 ° C for 24 hours. The filtrate was concentrated by using a vacuum distillation apparatus (EYELA, JP / N-1000), and then lyophilized to obtain 42 g of a dried extract. The extracts were sterilized by irradiation with 60Co gamma rays (10 kGy / H, 25 kGy).

Manufacturing example  8. Preparation of windproof extract

5 L of distilled water was added to 500 g of dried windshield, and the mixture was heated with stirring at 75 DEG C for 24 hours. The filtrate was filtered through a paper filter, Watman # 2, to remove the solids. The filtrate was concentrated using a vacuum distillation apparatus (EYELA, JP / N-1000) and lyophilized to obtain 46 g of a windshield extract. The extracts were sterilized by irradiation with 60Co gamma rays (10 kGy / H, 25 kGy).

Manufacturing example  9. Preparation of the extract

5 g of distilled water was added to 500 g of dried persimmon and heated at 75 캜 for 24 hours. The filtrate was concentrated by using a vacuum distillation apparatus (EYELA, JP / N-1000) and then lyophilized to obtain 42 g of the extract. The extracts were sterilized by irradiation with 60Co gamma rays (10 kGy / H, 25 kGy).

Manufacturing example  10. Manufacture of Viroxx extract

To 500 g of the dried glacial acetic acid, 5 L of distilled water was added and heated at 75 캜 for 24 hours. The filtrate was concentrated by using a vacuum distillation apparatus (EYELA, JP / N-1000), and then lyophilized to obtain 44 g of an albedo extract. The extracts were sterilized by irradiation with 60Co gamma rays (10 kGy / H, 25 kGy).

Manufacturing example  11. Manufacturing example  18. Bergamot  Preparation of Mixed Extracts of Extracts, Gold Euphoria Extracts, Yulmu Extracts and Milk Extracts

Bergamot extract, gingkojum extract, yulmu extract and wheat extract were mixed in the weight ratios shown in Table 1 below to obtain a mixed extract. Specifically, each of the extracts obtained in Preparation Examples 1 to 10 was dissolved in a tertiary distilled water at a concentration of 20 mg / ml, and each extract solution was mixed with the respective aqueous extract solutions so that the weight ratio of each extract was as shown in Table 1.

Weight ratio (%) of each extract component in the mixed extract Manufacturing example Bergamot extract Gold Euphoria extract Yulmu extract Germ extract 11 50 50 0 0 12 50 0 50 0 13 50 0 0 50 14 0 50 50 0 15 0 50 0 50 16 0 0 50 50 17 35 35 30 0 18 25 25 25 25

Manufacturing example  19. Manufacturing example  23. Bergamot  Extract, Ganoderma extract, Yulmu extract, Grape extract, Machi  Preparation of mixed extracts further comprising a wind-resistant extract, a herb extract or a vinegar extract in a mixed extract of an extract, a lycopene extract, and a wild-caught extract

Bergamot extract, giitta extract, yulmu extract, germ extract, horsetail extract, chrysanthemum extract, and wild ginseng extract were mixed at the same weight ratio to obtain a mixed extract. Specifically, each of the extracts obtained in Preparation Examples 1 to 10 was dissolved in a third distilled water at a concentration of 20 mg / ml, and each extract solution was mixed with each extract solution so that the weight ratio of each extract was as shown in Table 2. [

Weight ratio (%) of each extract component in the mixed extract Manufacturing example Bergamot extract Gold Euphoria extract Yulmu extract Germ extract Marchia extract Dried extract Wild chrysanthemum extract Windshield extract Hanchuncho extract Peony extract 19 14 14 14 14 14 14 16 20 12.5 12.5 12.5 12.5 12.5 12.5 12.5 12.5 21 12.5 12.5 12.5 12.5 12.5 12.5 12.5 12.5 22 12.5 12.5 12.5 12.5 12.5 12.5 12.5 12.5 23 11 11 11 11 11 11 11 11.5 11.5

Experimental Example  1. Check wrinkle improvement effect

1.1. Collagen biosynthesis promoting effect experiment

In order to confirm the effect of the mixed extract used as a skin wrinkle remedy in the present invention, the effect of collagen biosynthesis in human fibroblasts was examined by the following method using retinoic acid as a positive control substance. First, human fibroblasts were seeded on a plate with 6 wells and cultured for 24 hours. The culture was incubated with serum-free culture medium for 16 hours, and the test substances were added to each well containing 0.05% fetal bovine serum (FBS; FBS; 2 μg / ml, 10 μg / ml and 20 Mu] g / ml, and then cultured again for 48 hours.

After 48 hours, the cell culture medium was collected, and 100 ㎕ of each sample was put into each well of an antibody-coated plate and cultured at 37 캜 for 2 hours. Each well was washed with 400 μl of phosphate buffer solution (PBS), 100 μl of Solution I (Antibody-Peroxidase conjugate solution) was added to each well, incubated at 37 ° C. for 1 hour, Washed with 400 [mu] l PBS. 100 μl of a substrate solution (TMBZ, 3, 3 ', 5, 5'-Tetramethyl benzidine) was added to each well, followed by incubation at room temperature for 15 minutes. Then, 100 μl of 1 N sulfuric acid was added to each well, mixed well, and then analyzed by ELISA reader (enzyme-linked immunosorbant assay) at 450 nm for analysis. The positive control The results obtained are shown in Table 3 and Fig. 1 in comparison with retinoic acid.

In FIG. 1, the negative control is a test compound that contains 100% of the collagen production amount when no wrinkle improving substance is added, and the test substances such as bergamot extract, ginger extract, yulmu extract, moss extract, Extracts, windshield extracts, licorice extracts, peony extracts or their mixed extracts and retinoic acid, a positive control.

As shown in the following Table 3, the retinoic acid used showed an increase effect of 21.6% at a concentration of 10 占 퐂 / ml. On the other hand, the mixed extract showed an increase of 27.2% at 10 占 퐂 / ml (Production Example 23), confirming that collagen biosynthesis is promoted more than retinoic acid.

Promoting collagen biosynthesis
The amount of collagen (% compared with the control) 2 Mu g / ml 10 Mu g / ml 20 Mu g / ml Production Example 1 103.6 106.9 115.6 Production Example 2 103.1 105.8 113.8 Production Example 3 101.5 104.2 110.2 Production Example 4 101.4 103.8 105.3 Production Example 5 103.8 110.2 116.8 Production Example 6 103.4 108.2 110.2 Production Example 7 102.5 106.4 111.6 Production Example 8 102.6 104.4 109.2 Production Example 9 102.1 107.6 110.3 Production Example 10 103.3 105.2 109.4 Production Example 11 103.5 105.8 112.8 Production Example 12 102.8 105.1 111.4 Production Example 13 101.8 104.2 110.8 Production Example 14 102.8 104.8 112.7 Production Example 15 102.8 104.8 112.8 Production Example 16 102.8 104.2 108.8 Production Example 17 104.8 108.8 118.8 Production Example 18 106.2 118.8 127.2 Production Example 19 107.2 119.6 128.8 Production example 20 107.8 120.4 130.2 Production Example 21 108.2 121.2 131.4 Production Example 22 108.0 120.8 130.8 Production Example 23 108.5 127.2 143.7 Retinoic acid 106.2 121.6 136.5 Negative control group 100 100 100

1.2. Collagenase  Active inhibition test

In order to examine the effect of the bergamot extract, Gingko Ehwa extract, Yulmu extract, Mushroom extract, Matsuchiyu extract, Lycoris spp., Wild mushroom extract, Windshield extract, To examine the effect of inhibiting collagenase activity promoting wrinkle formation. In order to measure the degree of inhibition of collagenase (MMP-1, matrix metal protease-1) activity, an antibody of collagenase was used. In this test, the type I collagenase assay kit Absorbance was measured by ELISA and tested by the following method.

First, human fibroblasts were cultured in a suitable medium at 37 ° C under 5% carbon dioxide. After that, a certain number of cells were dispensed in different culture vessels for a certain period of time, and cell adhesion was confirmed, followed by culturing in serum-free culture medium for 16 hours. The concentrations of bergamot, gingkohwaso extract, yulmu extract, mulberry extract, mashii extract, mulberry extract, mulberry extract, windshield extract, myrrhicin extract, vinegar extract or mixed extract thereof were 2 ㎍ / ㎖, 10 ㎍ / ㎖ , TNF-alpha (tumor necrosis factor) was added to 50 ㎍ / ml of collagenase-activating substance and cultured for 24 hours. The cell culture medium was collected and 100 ㎕ was added to a 96-well tissue culture plate (Corning, USA). Enzyme was added as a detection reagent to each well, followed by incubation at 37 캜 for 90 minutes. And measured at 450 nm. In FIG. 2, compared with the case where the wrinkle-improving raw material was not added in the negative control group, bergamot extract, gingerbread extract, yulmu extract, moss extract, mashin extract, chrysanthemum extract, And the collagenase inhibition efficiency (%) as the concentration of the extract increased.

As shown in Table 4 below, the concentration of bergamot extract, gingerbread extract, yulmu extract, mulberry extract, mashin extract, mulberry extract, mulberry extract, windshield extract, The activity of collagenase was inhibited as the concentration was increased to 50 / / ㎖ and 50 ㎍ / ㎖ of Preparation Example 23, and the highest collagenase inhibitory effect of 62.4% at 50 ㎍ / ㎖ of Preparation Example 23 was observed. Thus, bergamot extract, , Extracts of Mushroom, Lycoris sieboldii, Lycoris spp., Lycoris spp., Windshield extract, Hanshikoscho extract, and Vaccine extract inhibited collagenase activity.

Collagenase  Measurement of inhibition of activity
Collagenase  Active inhibition (%) 2 [mu] g / ml 10 [mu] g / ml 50 [mu] g / ml Production Example 1 4.8 8.4 17.8 Production Example 2 4.2 7.6 16.5 Production Example 3 2.6 6.1 13.9 Production Example 4 3.2 6.6 12.8 Production Example 5 5.1 9.1 18.7 Production Example 6 5.0 9.1 18.7 Production Example 7 3.8 7.8 16.2 Production Example 8 4.0 7.2 15.8 Production Example 9 3.8 7.8 16.2 Production Example 10 4.4 8.2 18.1 Production Example 18 10.2 20.6 44.6 Production Example 19 11.2 23.6 48.6 Production example 20 11.8 24.8 52.2 Production Example 21 12.2 23.9 53.4 Production Example 22 12.1 24.1 52.8 Production Example 23 15.3 28.8 62.4 TGF -β (50 ng / Ml) 57.4

1.3. Skin elasticity improvement effect

The compositions of Preparations 18, 19 and 23, which proved to be effective, were tested as follows to test the effect of improving the elasticity in real human skin. The skin elasticity was measured by using a skin elasticity tester (Cutometer SEM 575, C + K Electronic Co., Germany) after applying to the face in the morning and evening 2 times for 12 weeks. And the results are shown in Table 5 below. The results in Table 5 indicate the viscoelasticity of the skin elasticity measuring device. It was confirmed that the extract of Preparation Example 23 significantly improved the skin elasticity effect as compared with the control.

Measuring skin elasticity improvement effect Experimental group Skin elasticity effect Production Example 18 0.66 Production Example 19 0.76 Production Example 23 0.88 Control group 0.14

In addition, at the end of the test, subjects were asked to fill out questionnaires and perform subjective evaluation of efficacy at the same time as the device evaluation. The results are shown in Table 6.

Skin elasticity improvement effect survey result Application group
Number of Respondents (sign) Very good Good usually Inadequate Production Example 18 4 12 4 0 Production Example 19 6 11 3 0 Production Example 23 8 10 2 0 Control group 2 5 11 2

Experimental Example  2. Check the whitening effect

2.1. Tyrosinase  Measurement of inhibitory activity

Tyrosinase activity was used with minor modifications to the method of Pomerants S. H., 1963 J. Biol. Chem., 238, 2351-2357. 40 μl of a 5 mM L-DOPA solution, 80 μl of 68 mM phosphate buffer (pH 6.8) and 40 μl of a test sample solution (Preparation Examples 1 to 23 and a test solution concentration of arbutin of 5 mg / ml) . Next, 40 [mu] l (100 units / ml) of human tyrosinase was added to prepare a sample sample.

The final concentration of the test sample solution thus obtained was 1 mg / ml. To make the final concentration of the test solution 0.5 mg / ml, 40 μl of 5 mM L-DOPA solution, 100 μl of 67 mM phosphate buffer (pH 6.8) and 20 μl (Preparation Examples 1 to 23 and test of arbutin Solution concentration 2.5 mg / ml) were mixed, and then 40 μl (100 units / ml) of mushroom tyrosinase was added to the reaction mixture to prepare sample samples.

In the preparation of the sample, the test sample solution was not added and used as a control. When the enzyme activity of the control group was taken as 100, the enzyme inhibitory activity of each enzyme was expressed as% in comparison with the control group. In order to observe the relative intensities of the tyrosinase inhibitory activities of the compositions of Production Examples 1 to 23, the relative activities with respect to arbutin, which is widely used for whitening cosmetics, were compared. The percent inhibition of tyrosinase activity was calculated as follows.

Where A is the absorbance at 490 nm without the sample and B is the absorbance at 490 nm when the sample is present.

Tyrosinase  Measure inhibitory activity sample Content (mg / ml) Inhibitory activity (%) Arbutin
0.5 20.2 One 52.4 Manufacturing example  One 0.5 10.4 One 17.5 Manufacturing example  2
0.5 12.0 One 18.6 Manufacturing example  3 0.5 5.8 One 8.5 Manufacturing example  4
0.5 6.2 One 10.5 Manufacturing example  5
0.5 11.2 One 18.2 Manufacturing example  6
0.5 9.2 One 12.5 Manufacturing example  7
0.5 7.4 One 11.8 Manufacturing example  8 0.5 8.2 One 13.2 Manufacturing example  9
0.5 9.8 One 16.7 Manufacturing example  10
0.5 8.4 One 15.2 Manufacturing example  18 0.5 16.8 One 34.2 Manufacturing example  19 0.5 17.2 One 45.7 Manufacturing example  20 0.5 17.6 One 44.8 Manufacturing example  21 0.5 18.2 One 49.2 Manufacturing example  22 0.5 18.6 One 48.6 Manufacturing example  23 0.5 20.5 One 51.5

2.2. Melanin synthesis inhibitory effect

2.2.1. B16 Melanoma  Cell culture

B16 melanoma cells were purchased from KTCC (The Korean collection for Type Culture). B16 Melanoma cells are melanin-producing cell lines and are generally used for screening whitening substances.

DMEM medium containing 10% FBS, 1% penicillin / streptomycin was prepared and sterilized to sterilize the medium for culturing the cells. Cell cultures were incubated in an incubator at 37 ° C, 5% CO ₂ . Melanoma cells were cultured for about 3 days and passaged when 100 ㎎ Patrydish was about 90% full.

2.2.2. Measurement of inhibition of melanin synthesis by absorbance measurement

In order to determine the whitening effect of bergamot extract, gingerbread extract, yulmu extract, germanium extract, mashin extract, chrysanthemum extract, wild ginseng extract, windshield extract, After the same amount of each extract or arbutin was treated, the change in the amount of melanin produced was measured. Cells were inoculated in a 96-well plate. After 24 hours, the extracts or arbutin prepared in Preparation Examples 18 to 23 were treated at a constant concentration for 48 hours, and cells were recovered using trypsin-EDTA. The recovered cells were reacted with 1 N NaOH containing 10% DMSO (dimethyl sulfoxide) at 80 ° C for 1 hour to allow the melanin to fully dissolve. Then, the cells were subjected to enzyme-linked immunosorbent assay (ELISA) Absorbance was measured at 405 nm. Melanin production was calculated using the following equation (2). As a result, the amount of melanin was 73.6% at the concentration of 500 ㎍ / ㎖, compared with that of the untreated group, which is known as a general whitening substance, but the content of bergamot extract, It was confirmed that the amount of melanin was lowered at a concentration of 500 ㎍ / ㎖ in the mixed extracts of Chinese cabbage extract, windshield extract, In particular, in Production Example 23, 75.2% was produced (Table 8 and FIG. 3).

Measurement of inhibition of melanin synthesis content Arbutin Production Example 18 Production Example 19 Production example 20 Production Example 21 Production Example 22 Production Example 23 Melanin production rate
(%)
0.5 mg / ml 73.6 84.4 82.2 79.2 80.6 78.8 75.2 1 mg / ml
47.8 76.2 72.4 69.6 67.2 68.4 51.8

2.2.3. Test for whitening effect on human skin

Twenty healthy men and women aged 25 years or older who were healthy as subjects underwent an opaque tape with 6 holes of 2 cm in diameter on the upper arm of the subject and they were exposed to ultraviolet rays (UVB) 1.5 to 2 times the minimum erythema of each subject After the induction of the blackening of the skin, the test materials were applied, and the darkness and darkness of the skin were measured using a colorimeter every two weeks from one month after. Each sample was dissolved in a vehicle of propylene glycol / ethanol (8/2) at a concentration of 1%, and was perfected daily twice a day (morning and evening). The intensity of skin was expressed as 'L' (In Korean) is usually between 50 and 70. When each sample is applied, the L value gradually increases when the sample is effective, and the comparison between the samples is ΔL (Final L value-value immediately before application of the sample) (FIG. 4).

Measurement of whitening effect on human skin L value 0 weeks 4 weeks 6 weeks 8 weeks Control group (no addition ) 57.32 + - 2.31 57.55 ± 2.26 57.60 ± 2.13 57.75 + - 2.62 Arbutin 57.22 + - 2.25 58.54 + - 2.69 59.82 ± 2.61 60.68 + - 2.34 Production Example 18 57.62 + - 2.42 58.48 + - 2.51 59.02 + - 2.42 59.48 ± 3.12 Production Example 19 57.43 + - 2.25 58.32 ± 1.86 59.18 ± 2.21 59.56 ± 2.58 Production Example 23 57.51 ± 2.23 58.78 + - 2.52 59.58 ± 2.27 60.24 + - 2.62

* The above values are expressed as mean ± standard deviation.

As can be seen from the results shown in Table 9 and FIG. 4, the mixed extracts of bergamot extract, giitta extract, yulmu extract, mushroom extract, mashin extract, chrysanthemum extract, wild mushroom extract, windshield extract, Similar or superior whitening effect.

Experimental Example  3. Identify damaged skin's ability to heal

3.1. Fibroblast Fibroblast ) ≪ / RTI >

Human fibroblasts cultured in DMEM (Doubecco's Modified Eagle's Media) medium containing 3.5% fetal bovine serum were dispensed in a 96-well microtiter plate to a concentration of 5,000 cells / well. To 10, 18 to 23 and FBS (Fetal bovine serum) as a control were added in an amount of 1%, respectively, and then cultured at 37 DEG C for 2 days. After the incubation, 50 μl of 0.2% MTT (3- [4,5-dimethylthiazol-2-yl] -2,5-diphenyltetrazolium bromide) solution was added to each well and incubated at 37 ° C for 4 hours. The resulting formazane was dissolved in DMSO (Dimethylsulfoxide). The absorbance of dissolved formazan was measured at 570 nm using a microplate reader. Cell proliferation was evaluated for each sample through this MTT test. The results are shown in Table 2.

sample cell Proliferative ability (%) Control group 30.21 Production Example 1 14.4 Production Example 2 15.3 Production Example 3 14.1 Production Example 4 16.2 Production Example 5 14.6 Production Example 6 17.3 Production Example 7 16.5 Production Example 8 17.1 Production Example 9 17.6 Production Example 10 15.9 Production Example 18 24.5 Production Example 19 26.6 Production example 20 25.8 Production Example 21 25.5 Production Example 22 26.7 Production Example 23 28.4

It can be seen from Table 10 and FIG. 5 that when the mixed extracts of bergamot extract, giitta extract, yulmu extract, wheat extract, horseradish extract, chrysanthemum extract, geranium extract, windshield extract, Very high.

3.2. Reckless mouse  Measuring skin barrier function recovery effect in skin

In order to measure the effects of the mixed extracts obtained from Preparation Examples 18, 19 and 23 on the recovery of skin barrier function damaged by skin damage and on the increase in skin moisturizing ability, the following experiment was conducted.

First, acetone was applied to the mice twice a day for 5 days periodically, and the loss of barrier function of the skin such as an experimental animal was induced by an evaporimeter (Charles River, Japan) at 8 to 10 weeks after birth Propane glycol and ethanol were mixed in a ratio of 6: 4 as a test substance only to experimental animals having skin showing a transdermal water loss (TEWL) of 40 g / m ² / hr or more. A sample containing 1% of each of the extracts obtained from Vehicle (vehicle-treated group) and each of the extracts obtained from Preparations 18, 19 and 23 or their mixed extracts was administered twice at a dose of 200 μl per 5 cm ² of skin area TEWL was measured at constant time while continuously applying for 3 days, and the results are shown in Table 11 and FIG.

sample
TEWL 0h 6h 12h 24h 48h Production Example 18 48.6 46.8 43.6 34.2 28.5 Production Example 19 47.6 44.5 41.2 29.8 22.6 Production Example 23 46.8 43.8 38.5 27.8 19.8 Control group ( Purified water ) 46.2 40.8 34.8 24.8 17.5

From the above results, it was confirmed that the mixed extracts of Production Examples 18 to 23 were excellent in the promotion of differentiation of skin cells, inhibition of proliferation and recovery of damaged skin barrier.

Experimental Example  4. Confirm skin safety

4.1. Toxicity test

The cytotoxicity tests of the mixed extracts of bergamot extract, giitta extract, yulmu extract, mulberry extract, horsetail extract, mulberry extract, mulberry extract, windshield extract, haniksikcho extract, and albedo extract of Preparations 18-23 were carried out. Cells were inoculated in a 96-well plate, and after 24 hours, the black mold extract was treated at a constant concentration and incubated in an incubator of 5% CO ₂ at 37 ° C. After culturing for 48 hours, the medium was removed and 100 μl of a solution of MTT (3- (4,5-dimethythiazol-2-yl) -2,5-diphenyltetrazoliu, bromide) was added and reacted at 37 ° C for 4 hours. After the reaction was completed, the solution was removed, 100 쨉 l of DMSO was added to dissolve the stained cells, and the absorbance at 540 ㎚ was measured using an ELISA reader. Cell viability was calculated using the following equation (2). As a result, the extracts (0.5 mg / ml or 1 mg / ml) of bergamot extract, gingerbread extract, yulmu extract, wheat extract, mashin extract, Ml), the cell survival rate was 97.6% to 98.8% (Table 12 and Fig. 7) when compared with the control.

Cytotoxicity measurement of mixed extracts content Untreated group Arbutin Production Example 18 Production Example 19 Production example 20 Production Example 21 Production Example 22 Production Example 23 cell survival rate
(%)
0.5 mg / ml 100 98.8 98.8 98.6 98.2 98.4 98.5 98.1 1 mg / ml
100 97.8 98.1 97.9 97.8 97.6 97.8 97.6

The composition of the present invention can be prepared by the following formulation, and the following formulation exemplifies the present invention, and thus the content of the present invention is not limited thereto.

4.2. Evaluation of skin safety

In order to confirm the skin safety of the composition of the present invention, the skin stability of the composition of the present invention was evaluated by performing a patch test in which 20 adult women and 20 men were applied.

Twenty - eight hours after the application of the patch, the patch was removed, and the first reading was performed 30 minutes later and the second reading after 96 hours. To determine the intensity of the skin irritation of the sample, weights were assigned according to the degree of the positive reaction of the skin, and the average skin response was obtained to visually determine the skin irritation of the sample. The results are shown in Table 13 below.

Test substance Average reactivity Judgment grade Production Example 18 0.00 No stimulation Production Example 19 0.00 No stimulation Production example 20 0.00 No stimulation Production Example 23 0.00 No stimulation

As shown in Table 13, all of Preparation Examples 18 to 20 and Preparation Example 23 do not stimulate the skin. Therefore, the cosmetic composition of the present invention can be judged to have excellent skin safety.

Formulation example  1. Flexible lotion

Examples of the preparations of the flexible lotion containing the mixed extracts of Bergamot, Gingkohwil, Yulmu, Maesil, Maehyunhyang, Baekjung, Ganhwasa, Hansikucho and Viroxx were prepared according to the composition of the following Table 14 according to the present invention.

ingredient Content (% by weight) Mixed extract of bergamot, ganghwase, yulmu, wheat germ, mashinhyeong, bokjung, ginkgohwa, 4.0 Polyoxyethylene hardened castor oil 0.4 Glycine 3.5 Dipotassium glycyrrhizate 0.1 1,3-butylene glycol 3.0 Sodium hyaruronate 0.1 ethanol 5.0 Antioxidant 0.1 Triethanolamine 0.1 EDTA 0.1 Spices 0.05 Purified water Balance

Formulation example  2. Nourishing Lotion

The preparation examples of the nutritional lotion containing the mixed extracts of bergamot, gingkohwil, yulmu, mulch, mackinjia, mulberry, mulberry, hanchikwon, and vinegar according to the present invention were prepared as shown in the following Table 15.

ingredient Content (% by weight) Mixed extract of bergamot, ganghwase, yulmu, wheat germ, mashinhyeong, bokjung, ginkgohwa, 4.0 glycerin 7.0 Sorbitan stearate sucrose cocoate 2.0 Mineral oil 4.0 Trioctanoin 1.0 Stearic Acid 1.0 Glyceryl stearate 0.5 Sorbitan monostearate 1.0 Dimethicone 0.5 Antioxidant 0.2 Triethanolamine 0.1 Carbomer 0.2 EDTA 0.1 Spices 0.05 Purified water Balance

Formulation example  3. Nutritional Essence

Examples of formulations of nutritional essences containing a mixed extract of bergamot, gingkojum, yulmu, mugwort, majiwon, mulberry, mulberry, hansikucho, and vinegar according to the present invention were prepared as shown in the following Table 16.

ingredient Content (% by weight) Mixed extract of bergamot, ganghwase, yulmu, wheat germ, mashinhyeong, bokjung, ginkgohwa, 7.0 glycerin 5.0 1,3-butylene glycol 2.0 Polyethylene glycol 2.0 Carbomer 1.0 Sodium hydrulonate 0.1 Glycine 3.0 Polyacrylamide 2.0 Hydroxyethyl cellulose 0.2 ethanol 3.0 Polyoxyethylene hardened castor oil 1.0 Antioxidant 0.3 Triethanolamine 0.1 EDTA 0.1 Spices 0.05 Purified water Balance

Formulation example  4. Nourishing Cream

Examples of formulations of nutritional creams containing mixed extracts of bergamot, gingkojum, yulmu, mugwort, majiwon, mulberry, mulberry, hansikucho, and vinegar according to the present invention were prepared as shown in the following Table 17.

ingredient Content (% by weight) Mixed extract of bergamot, ganghwase, yulmu, wheat germ, mashinhyeong, bokjung, ginkgohwa, 10.0 1,3-butylene glycol 3.0 glycerin 3.0 Hydrogenated lecithin 1.0 Octyldodecanol 3.0 Trioctanoin 2.0 Stearic Acid 1.5 Cetostearyl alcohol 2.0 Polysorbate 60 1.5 Sorbitan sesquioleate 2.0 Dimethicone 3.0 Antioxidant 0.3 Xanthan gum 0.2 Triethanolamine 0.1 EDTA 0.1 Spices 0.05 Purified water Balance

The present invention provides a cosmetic composition containing, as an active ingredient, bergamot extract, gingerbread extract, yulmu extract, moss extract, mashin extract, chrysanthemum extract, geranium extract, windshield extract, But it is also excellent in the recovery of damaged skin tissues and is utilized as a multi-functional cosmetic composition.

Claims (12)

A multifunctional cosmetic composition for improving wrinkles and whitening comprising bergamot extract, gingerberry extract, yulmu extract, and wheat extract. The multi-functional cosmetic composition for wrinkle improvement and whitening according to claim 1, wherein the bergamot extract, gingerberry extract, yulmu extract and wheat extract are contained in a ratio of 1 to 10: 1 to 10: 1 to 10: 1 to 10 . The multifunctional cosmetic composition according to claim 1, wherein the extract is extracted using a solvent selected from the group consisting of water, a C ₁ to C ₃ alcohol, and a mixture thereof. The multifunctional cosmetic composition according to claim 1, further comprising a marigold extract, a liquorice extract and a marigold extract. The multi-functional cosmetic composition according to claim 4, wherein the cosmetic composition further comprises at least one selected from the group consisting of a windshield extract, a myrrhicin extract, and a vile extract. A functional cosmetic composition for accelerating the recovery of damaged skin comprising bergamot extract, gingerol extract, yulmu extract, and wheat extract. [Claim 7] The functional cosmetic composition according to claim 6, wherein the bergamot extract, ginger extract, yulmu extract, and wheat extract are contained at a ratio of 1 to 10: 1 to 10: 1 to 10: 1 to 10. The cosmetic composition according to claim 6, wherein the extract is extracted using a solvent selected from the group consisting of water, C ₁ to C ₃ alcohol, and mixtures thereof. The functional cosmetic composition according to claim 6, which further comprises a marigold extract, a liquorice extract and a marigold extract. The functional cosmetic composition for promoting damaged skin recovery according to claim 9, which further comprises any one selected from the group consisting of windshields, hanchikcho, and vinegar. A multi-functional cosmetic composition for promoting wrinkle improvement, whitening, and damaged skin recovery comprising bergamot extract, gingerbread extract, yulmu extract, ginseng extract, mashin extract, chrysanthemum extract, geranium extract, The cosmetic composition according to any one of claims 1 to 11, wherein the cosmetic composition is formulated with a cream, a softening agent, a convergent lotion, a milk lotion, a foundation, a soap or a cleansing foam.

Images