KR101303295B1 - The cosmetic composition for promoting cell growth and recovery of distrupted skin containing the extracts of fermented Mistletoe, fermented Imperata cylindrica and fermented soybean - Google Patents

Abstract

The present invention relates to a fermentation cosmetic composition for promoting cell proliferation and promoting damaged skin, including mistletoe fermentation extract, hair root fermentation extract and soybean fermentation extract. The cosmetic composition of the present invention is excellent in measuring the proliferative effect of fibroblasts and promoting the differentiation of keratinocytes, and also excellent in promoting the recovery of skin tissue damage caused by chemicals or photostimulation. A composition can be provided.

Description

The cosmetic composition for promoting cell growth and recovery of distrupted skin containing the extracts of fermented Mistletoe, fermented, including mistletoe fermented extract, hair root fermented extract and soybean fermented extract as active ingredients Imperata cylindrica and fermented soybean}

The present invention relates to a fermentation cosmetic composition having an effect of promoting cell proliferation and promoting damaged skin repair.

The skin plays a very important role as a barrier function to protect individuals from the outside. Barrier function is to protect against various stimuli (chemicals, air pollutants, dry environment, ultraviolet rays, etc.) from the outside and to prevent excessive release of moisture in the body through the skin. This protective function is a stratum corneum composed of keratinocytes. The function can be maintained only when is normally formed.

The outermost stratum corneum (horneylayer) of the epidermis is formed from keratinocytes and consists of the differentiated keratinocytes and the surrounding lipid layer (Marcelo C. L. et al, J. Invest. Dermatol., 80 , pp 37-44,1983).

Keratinocytes are characteristic cells whose basal cells, which continuously proliferate in the epidermal layer, undergo morphological and functional changes in stages and rise to the surface of the skin. After a period of time, old keratinocytes are eliminated from the skin. New keratinocytes take over their functions, and this repetitive process of change is called "epidermal differentiation" or "keratinization".

In addition, during keratinization, keratinocytes form a stratum corneum while producing natural moisturizing factors and intercellular fats (ceramides, cholesterol, fatty acids) so that the stratum corneum has firmness and flexibility to retain its function as a skin barrier.

The stratum corneum can easily lose its function due to excessive face washing, lifestyle factors such as bathing, environmental factors such as dry air pollutants, and endogenous diseases such as atopic or aged skin. Recently, due to a variety of factors, more and more skin complaints and dry skin complaints are increasing in recent years.

However, in recent years, the risk factors for the skin have been gradually increased, the changes in dietary patterns slow down the formation and dropping of the stratum corneum, and the amount of moisturizing factors and lipids in the stratum corneum is reduced due to the deterioration of the function of keratinocytes. There is a growing number of people with skin that does not exhibit skin barrier function. This disruption of skin barrier function causes various skin diseases such as dry skin, atopic dermatitis, contact dermatitis and psoriasis. These diseases can be expected to alleviate the symptoms with a conventional moisturizer having a conventional water retention function, but fundamental healing is difficult.

Soybean Edible crops are widely cultivated, also known as soybeans. Soybean (Glycine max (L.) Merrill) is a botanical plant belonging to the species Legumes (Leguminosae), Papilionoideae and Glycine L., with seeds containing 36-45% protein and 17% oil. 22%, in addition to triterpenoids saponin, nitrogen compounds, carbohydrates, candy, pectin, minerals and vitamins. 80-90% of the protein consists of essential amino acids such as arginine, methionine, threonine, leucine, isoleucine, valine, etc. The phospholipid content of seeds contains lecithin, kephalin, etc., and nitrogen compounds contain adenine, guanine, choline, trigonelin, and histidine, an amino acid (interest, ingredient and use of herbal medicine, p. 187, 1999). ).

Mistletoe (Viscum album) is a plant belonging to the family Mistletoe, which is known to have 30 genera and 1,500 species around the world. Mistletoe used as a medicament among several species of mistletoe is a mistletoe of the genus Viscum. In particular, the European mistletoe Viscum album loranthacea is already used as an anticancer and anticancer supplement in Europe. The Korean mistletoe is a species of Viscum album coloratum that differs from the European mistletoe. It is a parasitic plant that hosts various trees such as oak, pear, alder, apple, hackberry, pine and spruce. In herbal medicine, the herb life has been used for soothing, pain, pain, low back pain, high blood pressure, small swelling, prevention of miscarriage, toothache, pain. In addition, Lactobacillus fermenting mistletoe is known in Europe, Lactobacillus plantarum (Lactobacillus plantarum), it is reported that the mistletoe fermented with the lactic acid bacteria has an immune enhancing effect to stimulate the humoral and cellular immune system. Mistletoe fermentation by lactic acid bacteria is known to reduce the direct toxic effect of mistletoe on the cells, while maintaining the enhancer activity of mistletoe.

In addition, the band (Imperata cylindrica var.koenigii) is a perennial herb with monocotyledonous plant rice plants and is distributed throughout the temperate and tropical regions of North America, Asia, and Africa. The stem comes out from the node of the rhizome and is flat and rows, 20 ~ 50 cm long, 7 ~ 12 mm wide, with pointed end and surrounded by 12 mm long feathers. The Chinese herb is called Baekmo-geun, and it is a perennial herb that is used as a medicinal herb to remove heat, urinate well, and treat jaundice and tree species in Chinese herbal medicine nuclear power plants. It grows on the coast and fields of Jeju Island. It has been fed not only as an old plant but also to a patient with jaundice, and its stem has been regarded as a valuable material for its thatched roof.

The present inventors have completed the present invention by finding a plant extract showing excellent cell proliferation ability during the study on the cosmetic composition that can prevent more efficient skin damage.

KR 10-2000-38058A Green KR 10-2008-54829A Abstract

Accordingly, it is an object of the present invention to provide a fermentation cosmetic composition for promoting cell proliferation or promoting damaged skin, comprising an effective fermentation extract of herbal medicine or a combination thereof as an active ingredient.

In order to achieve the above object, one embodiment of the present invention provides a fermentation cosmetic composition for promoting cell proliferation and / or damaged skin recovery comprising a yeast fermentation extract of the hair root as an active ingredient.

The hair root yeast fermentation extract may be obtained by severing the hair roots, inoculating yeast in the cut hair roots, or obtaining an extract from the hair roots, and then inoculating the yeast strains and fermenting it again.

The extract can be prepared using conventional methods known in the art, i.e., under conventional temperature and pressure conditions, using conventional solvents. Extraction solvents that can be used to prepare the hair root extract of the present invention include, for example, water, lower alcohols having ₁ to ₃ carbon atoms, acetone, ethyl acetate, butyl acetate and 1,3 butylene glycol alone. Can be used or mixed. A suitable amount of the extraction solvent is about 1 to 15 times the dry weight of the hair root extract, may be 5 to 12 times, and may be 10 times, but is not limited thereto. In addition, as the extraction method, heat extraction, cold needle extraction, reflux cooling extraction, ultrasonic extraction, and the like may be used, and may be extracted once or repeatedly. The extraction temperature is not particularly limited as long as the extraction temperature is such that the effective activity of the useful component of the hair root extract is not removed.

The yeast fermentation can be carried out by conventional methods known in the art. Depending on the characteristics of each strain can be cultured under conventional conditions and aerobic or usually anaerobic conditions. In addition, additional carbon sources and / or pH adjusters may be added to further activate the culture of the fermentor. For example, the extract or unfiltered extract is inoculated with yeast at a constant concentration of 10 ⁵ to 10 ⁷ cells / ml, and maintained at a pH of 5.5 to 8.5, a temperature of 32 to 40 ° C, and a humidity of 60 to 80%. Depending on the culture can be fermented for 24 to 96 hours. The yeast cells remaining in the resulting fermentation products are all sterilized under autoclave conditions such as an okclave.

The yeast may be used Saccharomyces sp., Shigazoaccharomyces sp., Etc., Saccharomyces cerevisiae, Saccharomyces cerevisiae, Schizosaccharomyces pombe, Klureromyces lactis, Saccharomyces boulardii, but are not limited thereto.

The hair root yeast fermentation extract is excellent in measuring the proliferative effect of fibroblasts (Fibroblast) and promoting the differentiation of keratinocytes (Table 2, Table 3, Figures 1 and 2), skin tissue by chemical substances or photostimulation It was confirmed that the effect of promoting the recovery of damage is excellent (see Table 4, Table 5, Figures 3 and 4).

In addition, one embodiment of the present invention provides a fermentation cosmetic composition for promoting cell proliferation and / or damaged skin recovery comprising a hair root yeast fermentation extract and mistletoe yeast fermentation extract as an active ingredient.

The mistletoe yeast fermentation extract can be obtained in the same manner as the hair root yeast fermentation extract. The fermentation cosmetic composition contains the hair root yeast fermentation extract and mistletoe yeast fermentation extract in a weight ratio of 1 to 10: 1 to 10, or in a weight ratio of 2 to 8: 2: 2 to 8, or 4 to 6: 4 to 6 It may be contained in the weight ratio of. However, the content ratio may be mixed in various weight ratios according to the characteristics of the product.

The combination of the hair root yeast fermentation extract and the mistletoe yeast fermentation extract is significantly superior to the fermentation extract of the fibroblast (Fibroblast) proliferation effect and keratinocyte differentiation promoting effect (Table 2, Table 3, Figure 1 and Figure 2). Confirmed. In addition, it was confirmed that the effect of promoting the recovery of skin tissue damage by chemicals or photostimulation is also superior to the single fermentation extract (Table 4, Table 5, Figures 3 and 4).

In addition, one embodiment of the present invention provides a fermentation cosmetic composition for promoting cell proliferation and / or damaged skin recovery comprising a hair root yeast fermentation extract, mistletoe yeast fermentation extract and soybean lactobacillus fermentation extract as an active ingredient.

The soybean lactobacillus fermentation extract may be obtained by a method similar to the hair root yeast fermentation extract. However, Lactobacillus bacteria (Lactobacillus) is used as fermentation bacteria, and Lactobacillus bacteria Lactobacillus brevis, Lactobacillus casei, Lactobacillus plantarum, Lactobacillus ashdophilus (Lactobacillus acidophilus) ), Lactobacillus helveticus and Lactobacillus delbrueckii subspecies Lactobacillus bulgaricus, but are not limited thereto.

The fermentation cosmetic composition may comprise a hair root yeast fermentation extract, mistletoe yeast fermentation extract and soybean lactobacillus fermentation extract in a weight ratio of 1 to 10: 1 to 10: 1 to 10, 2 to 8: 2: 2 to 8: 2: It may be contained in a weight ratio of ~ 8, and may be contained in a weight ratio of 4 ~ 6: 4 ~ 6: 4 ~ 6. However, the content ratio may be mixed in various weight ratios according to the characteristics of the product.

The hair root yeast fermentation extract, mistletoe yeast fermentation extract and soybean lactobacillus fermentation extract cells have a proliferative effect of fibroblasts and keratinocyte differentiation promoting effect (Table 2, Table 3, Fig. 1 and Fig. 2) compared to the single fermentation extract Remarkably excellent. In addition, it was confirmed that the effect of promoting the recovery of skin tissue damage caused by chemicals or photostimulation is also very good compared to the single fermentation extract (Table 4, Table 5, 3 and 4).

In addition, one embodiment of the present invention is damaged skin containing the hair root yeast fermentation extract alone or a mixture of hair root yeast fermentation extract, mistletoe yeast fermentation extract, or hair root yeast fermentation extract, mistletoe yeast fermentation extract and soybean lactobacillus fermentation extract It provides a fermentation cosmetics having a recovery promoting effect.

Fermentation cosmetic composition according to an embodiment of the present invention may be contained in the product 1 to 30% by weight, or may be contained in 5 to 20% by weight.

Cosmetic compositions comprising the extract of the present invention may include, in addition to the extract, conventional adjuvants and carriers such as antioxidants, stabilizers, solubilizers, vitamins, pigments, or flavorings, and any formulation commonly prepared in the art. It can also be prepared as, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations and sprays, etc. It may be formulated as, but is not limited thereto. More specifically, it may be prepared in the form of a flexible lotion, nourishing lotion, nourishing cream, massage cream, essence, eye cream, hair tonic, shampoo, rinse, cleansing cream, cleansing foam, cleansing water, pack, spray or powder. .

When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

In the case where the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. Especially, in the case of a spray, a mixture of chlorofluorohydrocarbons, propane / Propane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol esters and polyoxyethylene sorbitan esters, and microcrystals are used as carrier components. Castle cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, isethionate, an imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

The cosmetic composition comprising the mistletoe fermentation extract, hair root fermentation extract and soybean fermentation extract of the present invention is excellent in measuring the proliferation effect of fibroblasts and promoting the differentiation of keratinocytes, and recovering skin tissue damage by chemicals or photostimulation. Since the promoting effect is also excellent, it can be usefully used for repairing damaged skin tissue.

Figure 1 is a graph of the proliferative effect of fibroblasts for hair root extract, mistletoe extract and soybean extract, hair root yeast fermentation extract, mistletoe yeast fermentation extract and soybean lactobacilli fermentation extract, respectively, and mixtures thereof.
Figure 2 is a graph of the keratinocyte differentiation promoting effect of the hair root extract, mistletoe extract and soybean extract, hair root yeast fermentation extract, mistletoe yeast fermentation extract and soybean lactobacillus fermentation extract, respectively, and mixtures thereof.
3 is a graph showing the effect of promoting recovery against skin damage due to chemicals for hair root yeast fermented extract, mistletoe yeast fermented extract and soybean lactobacillus fermented extract, respectively, and mixtures thereof.
Figure 4 is a graph of the effect of promoting recovery against skin damage due to photostimulation for each of the hair root yeast fermentation extract, mistletoe yeast fermentation extract and soybean lactobacillus fermentation extract and mixtures thereof.

Hereinafter, the present invention will be described in more detail based on the following Examples and Test Examples, but the present invention is not limited to the following Examples and Test Examples.

< Example  1> Preparation of Mistletoe Extract

5 kg of tertiary distilled water was added to 1 kg of dried mistletoe, extracted three times at 80 to 85 ° C. for 3 hours, filtered through a 400 mesh filter cloth, and filtered through a 0.45 μm filter. The filtrate was filtered under reduced pressure (EYELA, JP / N-). Concentrated and freeze-dried to obtain 83 g of mistletoe hot water extract. The prepared extract was used by irradiation sterilization with 60Co gamma rays (10 kGy / H, 25 kGy).

< Example  2> Preparation of Hair Root Extract

Dried roots (roots of the strip) were washed with purified water, and after cutting, 5 liters of tertiary distilled water was added to 1 kg of cut hair roots, extracted three times at 80-85 ° C. for 3 hours, filtered through a 400 mesh filter cloth, and filtered with a filter of 0.45 μm. The filtrate was concentrated using a distillation under reduced pressure (EYELA, JP / N-1000) and freeze-dried to obtain 89 g of hair root hydrothermal extract. The prepared extract was used by irradiation sterilization with 60Co gamma rays (10 kGy / H, 25 kGy).

< Example  3> Preparation of Soybean Extract

To obtain the soybean extract, the soybeans were washed with purified water and dried, and then finely powdered, 5 liters of tertiary distilled water was added to 1 kg of soybean powder, and extracted three times at 80 to 85 ° C for 3 hours, followed by filtration with 400 mesh filter cloth. The filtrate was concentrated using a 0.45 μm filter, and the filtrate was concentrated using a vacuum distillation machine (EYELA, JP / N-1000), and then freeze-dried to obtain 108 g of soybean hydrothermal extract. The prepared extract was used by irradiation sterilization with 60Co gamma rays (10 kGy / H, 25 kGy).

< Example  4> Preparation of Mistletoe Fermented Yeast Extract

The dried mistletoe was washed with purified water, and after cutting, the dried mistletoe was added 5 liters of tertiary distilled water to 1 kg, extracted three times at 80 to 85 ° C. for 3 hours, filtered through a 400 mesh filter cloth, and filtered with a filter of 0.45 μm. The filtrate was concentrated using a distillation under reduced pressure (EYELA, JP / N-1000), dried, and then sterilized with 500 ml of mistletoe extract with Co gamma rays (10 kGy / H, 25 kGy).

The extract obtained above was inoculated with yeast. The yeast was used Saccharomyces cerevisiae (added at 50,000 cfu / L). The culture was incubated at 36 ° C. for 3 days. After incubation, the culture was first filtered by centrifugation and a filter of 0.45 μm using a filtration membrane, followed by removal of the culture and debris, followed by secondary filtration using a 0.25 μm filtration membrane. The filtrate was concentrated using a vacuum distillation machine (EYELA, JP / N-1000), and then 73 g of mistletoe yeast extract was obtained.

< Example  5> Preparation of Yeast Fermented Extract of Hair Roots

Dried roots (roots of the strip) were washed with purified water, and after cutting, 5 liters of tertiary distilled water was added to 1 kg of cut hair roots, extracted three times at 80-85 ° C. for 3 hours, filtered through a 400 mesh filter cloth, and filtered with a filter of 0.45 μm. The filtrate was concentrated using a distillation under reduced pressure (EYELA, JP / N-1000), dried, and then sterilized by irradiation of 500 ml of hair root extract with Co gamma rays (10 kGy / H, 25 kGy).

The extract obtained above was inoculated with yeast. The yeast was used Saccharomyces cerevisiae (added at 50,000 cfu / L). The culture was incubated at 36 ° C. for 3 days. After incubation, the culture was first filtered by centrifugation and a filter of 0.45 μm using a filtration membrane, followed by removal of the culture and debris, followed by secondary filtration using a 0.25 μm filtration membrane. The filtrate was concentrated using a vacuum distillation machine (EYELA, JP / N-1000), and then 78 g of hair root yeast extract was obtained.

< Example  6> soybean Lactobacillus fermented extract  Produce

Soybean (soybean) was washed with purified water and dried, and then finely powdered, 5 liters of tertiary distilled water was added to 1 kg of soybean powder, extracted three times at 80 to 85 ° C. for 3 hours, and filtered through a 400 mesh filter cloth and 0.45 μm. The filtrate was concentrated using a vacuum distillation apparatus (EYELA, JP / N-1000), dried, and then dried. 500 ml of soybean extract was sterilized by irradiation with Co gamma rays (10 kGy / H, 25 kGy).

The extract obtained above was inoculated with Lactobacillus. At this time, Lactobacillus was used as Lactbacillus fermentum (added to 50,000 cfu / L). The culture was incubated at 36 ° C. for 3 days. After incubation, the culture was first filtered by centrifugation and a filter of 0.45 μm using a filtration membrane, followed by removal of the culture and debris, followed by secondary filtration using a 0.25 μm filtration membrane. The filtrate was concentrated using a vacuum distillation machine (EYELA, JP / N-1000), and then 98 g of soybean lactobacillus fermented extract was obtained.

< Example  7 to 14> Preparation of Mixed Extract

Mistletoe extract, hair root extract, soybean extract, mistletoe yeast fermentation extract, hair root yeast fermentation extract, and soybean lactobacillus fermentation extract obtained in Examples 1 to 6 were mixed in a weight ratio as shown in Table 1 to prepare a mixed extract. .

Example Mistletoe Extract Hair root extract Bean extract Mistletoe yeast Fermented extract Hair root yeast  Fermented Extract Bean Lactobacillus Fermented extract 7 50 50 - - - - 8 50 - 50 - - - 9 - 50 50 - - - 10 35 35 30 - - - 11 - - - 50 50 - 12 - - - 50 - 50 13 - - - - 50 50 14 - - - 35 35 30

< Experimental Example  1> fibroblasts ( Fibroblast Proliferation efficiency of

Human fibroblasts cultured in DMEM (Doubecco's Modified Eagle's Media) medium containing 3.5% fetal calf serum were dispensed to a 96-well microtiter plate at 5,000 cells / well, and Example 1 was used as a sample. FBS (Fetal bovin serum) was added in an amount of 1% as an extract and a control of 14 to 14, and then incubated at 37 ° C. for 2 days. After incubation, 50 μl of 0.2% MTT (3- [4,5-dimethylthiazol-2-yl] -2,5-diphenyltetrazolium bromide) solution was added to each well, followed by incubation at 37 ° C. for 4 hours. The resulting formazan was dissolved in DMSO (Dimethylsulfoxide). The absorbance of the dissolved formazan was measured at 570 nm using a microplate reader. The MTT test evaluated cell proliferation for each sample. The results are shown in Table 2.

sample cell Proliferative ability (%) Control group 29.31 Example 1 15.4 Example 2 16.3 Example 3 12.2 Example 4 24.1 Example 5 28.4 Example 6 26.3 Example 7 16.2 Example 8 15.1 Example 9 15.8 Example 10 15.9 Example 11 34.5 Example 12 32.5 Example 13 33.7 Example 14 39.4

From Table 2, it can be seen that when the fermented extract is treated, the proliferative effect of the fibroblasts is very high as compared with the case of the general extract. In particular, unlike the general extract, the fermentation extract is found to have a very high proliferative effect of fibroblasts when the fermentation extract is mixed alone.

< Experimental Example  2> keratinocyte differentiation promoting effect

In order to determine the keratinocyte differentiation promoting effect of the extract obtained in Examples 1 to 14, the amount of CE (Cornified Envelop) generated during the differentiation of keratinocytes was measured by using absorbance. First, the keratinocytes of the primary cultured human isolated from the epidermis of the newborn were put in a culture flask and adhered to the bottom, and then treated with 1% of the extract culture solution of Examples 1 to 14 below, and then the cells were 70 to the bottom area. It was incubated for 5 days until it grew about 80%. At this time, the low calcium (0.02 mM) treated group and the high calcium (1.5 mM) treated group were negative and positive controls, respectively.

Thereafter, the cultured cells were harvested, washed with PBS, and sonicated by adding 1 ml of 10 mM Tris-HCl buffer (pH7.4) containing 2% SDS and 20 mM DTT (Dithiothreitol). After crushing, after boiling, centrifugation, the precipitate was again suspended in 1 ml of PBS to measure the absorbance at 340 nm. Separately, a portion of the solution after the sonication was taken to measure the protein content and used as a reference when evaluating the degree of cell differentiation. The results are shown in Table 3 below.

sample Keratinocyte Ability to distinguish (%) Negative control (calcium 0.02 mM  solution) 100 Positive control (calcium 1.5 mM  solution) 207 Example 1 123 Example 2 129 Example 3 118 Example 4 146 Example 5 151 Example 6 148 Example 7 124 Example 8 126 Example 9 128 Example 10 127 Example 11 167 Example 12 161 Example 13 165 Example 14 172

From Table 3, it can be seen that when the fermented extract is treated, the effect of promoting keratinocyte differentiation is very high as compared with the case of the general extract. In particular, unlike the general extract, when the fermentation extract is mixed with the fermentation extract it can be seen that the keratinocyte differentiation promoting effect is very high compared to the treatment alone.

< Experimental Example  3> Hairless mice  Measurement of skin barrier function recovery effect on skin

In order to measure the effect of the fermentation extract or the mixture of the fermentation extracts obtained from Examples 4 to 6, and 11 to 14 on the recovery of the damaged skin barrier function and the increase of skin moisturizing power due to skin damage, The same experiment was performed.

First, acetone is periodically applied to the back of hairless mice (Charles River, Japan) 8 to 10 weeks after birth for 5 days twice a day to induce a loss of barrier function of the back of the experimental animal, and then with an evaporimeter. TEWL (Transepidermal Water Loss) was measured, and the test substance was mixed with propylene glycol and ethanol at a ratio of 7: 3 as a test material only for experimental animals with skin showing transdermal water loss of 40 g / m ² / hr or more. Samples containing 1% each of the vehicle (vehicle treated group) and each of the fermentation extracts or mixtures of the fermentation extracts obtained from Examples 4 to 6 and 11 to 14 at a dose of 200 μl per 5 cm ² of skin area. TEWL was measured at regular time while applying continuously twice a day for 3 days, and the results are shown in Table 4 and FIG. 3.

sample
TEWL 0h 6h 12h 24h 48h Example 4 47.4 43.5 40.2 28.4 20.7 Example 5 46.7 43.7 39.5 27.2 18.8 Example 6 47.3 43.2 39.2 28.7 19.5 Example 11 48.4 43.7 35.8 22.5 12.1 Example 12 47.7 45.3 38.4 25.2 13.9 Example 13 45.9 41.9 36.8 24.5 13.5 Example 14 46.2 40.5 29.7 24.5 13.5 Control group Purified water ) 47.6 45.8 43.1 31.2 24.5

From the above results, it was confirmed that the mixed extracts of Examples 11 to 14 have excellent effects in promoting the differentiation and proliferation of skin cells, promoting the differentiation of keratinocytes and repairing damaged skin barriers.

< Experimental Example  4> In optical contact  Damage recovery effect measurement test

The initial skin color of the irradiated site was measured using a skin pigment analyzer (Chromameter CM2002). After the UV irradiation, the damage skin recovery effect was measured by using a solar simulator to measure the minimum amount of erythema in both arms of 10 subjects. First, ultraviolet rays (UV) were irradiated to both arms of testers according to the FDA method to cause erythema.

After 8, 16, 24, and 48 hours after ultraviolet irradiation, the extracts of Examples 4 to 6 and 11 to 14 were applied to one arm of the tester, and distilled water was applied to the other arm. Skin color was measured before and after UV light. After UV irradiation, the whole skin color was adjusted to 100 and the erythema index was relatively measured. The Anova test statistical significance is p <0.05.

sample
Erythema index Erythema index ) 8 hours 16 hours 24 hours 48 hours Example 4 137.62 176.52 174.86 142.98 Example 5 131.36 170.12 167.41 141.52 Example 6 132.47 171.68 168.24 142.74 Example 11 121.82 141.34 139.26 124.72 Example 12 121.71 143.52 142.26 124.68 Example 13 123.36 145.74 147.74 132.47 Example 14 117.24 131.23 129.54 119.24 Control group Purified water ) 152.45 196.52 194.34 188.26

< Experimental Example  5> toxicity test

The cytotoxicity test of each fermented extract obtained from Examples 4 to 6 was carried out. After 24 hours of inoculation of cells into 96-well plates, each fermentation extract obtained from Examples 4 to 6 was treated at a constant concentration and cultured in an incubator at 37 ° C., 5% CO ₂ . After 48 hours of incubation, the medium was removed and 100 μl of MTT (3- (4,5-dimethythiazol-2-yl) -2,5-diphenyltetrazoliu, bromide) solution was added and reacted at 37 ° C. for 4 hours. After the reaction, the solution was removed, and 100 μl of DMSO was added to dissolve the stained cells, and then the absorbance was measured at a wavelength of 540 nm using an ELISA reader. Cell viability was calculated using Equation 1 below. As a result, the cells treated with the fermented extracts (0.5 mg / ml or 1 mg / ml) obtained from Examples 4 to 6 showed 96.2% to 98.3% cell viability when compared to the untreated group (Table 6).

Cytotoxicity of Extracts content Untreated group Example 4 Example 5 Example 6 Cell survival rate (%) 0.5 mg / ml 100 98.3 97.2 97.6 1 mg / ml 100 97.1 96.2 98.3

The composition of the present invention can be prepared in the following formulations, the following formulations are merely illustrative of the present invention, whereby the content of the present invention is not limited.

< Formulation example  1> flexible lotion

Butylene glycol, glycerin, polyoxyethylene hydrogenated pirage oil, betaine, citric acid, sodium citrate, and preservatives were added to the purified water, followed by stirring and dissolving. Mistletoe fermented extract, hair root fermented extract and soybean fermented extract according to Example 14 were added thereto, followed by sufficient stirring to prepare a flexible lotion containing the mixed extract according to the present invention. Table 7 shows the content of each component.

ingredient Content (% by weight) Mistletoe fermentation extract, hair root fermentation extract and soybean fermentation extract 4.0 Butylene glycol 3.5 glycerin 2.5 Polyoxyethylene hardened castor oil 0.1 ethanol 2.5 Betaine 1.0 Citric acid 0.01 Sodium citrate 0.03 antiseptic a very small amount Spices a very small amount Purified water Balance

< Formulation example  2> nutrition lotion

Mistletoe fermentation extract, hair root fermentation extract and soybean fermentation extract, butylene glycol, glycerin, carboxyvinyl polymer, arginine, preservative and purified water according to Example 14 were heated with stirring at 70 ℃ ~ 75 ℃. Squalane, butylene glycol dicaprylate / dicaprate, sorbitan stearate, polysorbate 60, glyceryl stearate and stearyl glycerate mixture, which were heated by stirring at 75 ℃ to 80 ℃ was added to emulsify After stirring, by adding a fragrance while cooling to about 45 ℃ while stirring, and then cooled to 30 ℃ and matured to prepare a nutrient cosmetics containing the mixed extract according to the present invention. The content of each component is shown in Table 8 below.

ingredient Content (% by weight) Mistletoe fermentation extract, hair root fermentation extract and soybean fermentation extract 6.0 Butylene glycol 8.0 glycerin 5.0 Squalane 4.0 Butylene Glycol Dicaprylate / Dicaprate 5.0 Sorbitan stearate 1.5 Polysorbate 60 1.0 Glyceryl stearate 0.5 Stearyl glycyrrhetinate 0.2 Carboxyvinyl polymer 0.1 Arginine 0.1 antiseptic a very small amount Spices a very small amount Purified water Balance

< Formulation example  3> nutrition essence

Cytocyterol, polyglyceryl-2-oleate, ceramide, ceteareth-4 and cholesterol were stirred and mixed, and then the mistletoe fermentation extract, hair root fermentation extract and soybean fermentation extract, dicetyl phosphate, After emulsifying by adding a mixed solution of concentrated glycerin and purified water, the mixture was cooled to 45 ° C. while stirring, and then, flavored with stirring, and cooled to 30 ° C. to mature. Carboxyvinyl polymer, xanthan gum and preservatives were added thereto, stabilized and aged to prepare a nutrient essence containing the mixed fermentation extract according to the present invention. Table 9 shows the content of each component.

ingredient Content (% by weight) Mistletoe fermentation extract, hair root fermentation extract and soybean fermentation extract 7.0 Sitosterol 1.7 Polyglyceryl-2-oleate 1.5 Ceramide 0.5 Setares-4 1.2 cholesterol 1.5 Dicetyl phosphate 0.4 Concentrated glycerin 5.0 Carboxyvinyl polymer 0.2 Xanthan gum 0.2 antiseptic a very small amount Spices a very small amount Purified water Balance

< Formulation example  4> nutrition cream

1,3-butylene glycol, glycerine, hydrogenated resin, octyldodecanol, trioctanoin, stearic acid, polysorbate 60, and sorbitan sesquioleate are stirred and mixed, and then Mistletoe fermented extract, hair root fermented extract and soybean fermented extract, triethanolamine, dimethicone, EDTA, glycerin, and purified water mixed solution according to Example 14 were added to emulsify, and then stirred and cooled by stirring at 45 ° C. It cooled to 30 degreeC and aged. Xanthan gum and preservatives were added thereto, stabilized, and aged to prepare a nourishing cream containing the mixed extract according to the present invention. Table 10 shows the content of each component.

ingredient Content (% by weight) Mistletoe fermentation extract, hair root fermentation extract and soybean fermentation extract 10.0 1,3-butylene glycol 3.0 glycerin 3.0 Hydrogenated lecithin 1.0 Octyldodecanol 3.0 Trioctanoin 2.0 Stearic Acid 1.5 Cetostearyl alcohol 2.0 Polysorbate 60 1.5 Sorbitan sesquioleate 2.0 Dimethicone 3.0 Antioxidant 0.3 Xanthan gum 0.2 Triethanolamine 0.1 EDTA 0.1 Purified water Balance

< Formulation example  5> pack

Glycerin, 1,3-butylene glycol, sodium hyaluronate, glycine, and polyacrylamide by stirring and mixing, the mistletoe fermentation extract, hair root fermentation extract and soybean fermentation extract according to Example 14, squalene, dimethicone, Triethanolamine and EDTA solution were added to emulsify and then cooled to 45 ° C. while stirring, followed by the addition of fragrance, followed by cooling to 30 ° C. for aging. Xanthan gum and preservatives were added thereto, stabilized, and then aged to prepare a pack by applying the mixed extract according to the present invention to a nonwoven fabric. Table 11 shows the content of each component.

ingredient Content (% by weight) Mistletoe fermentation extract, hair root fermentation extract and soybean fermentation extract 8.0 glycerin 7.0 1,3-butylene glycol 3.0 Squalene 3.0 Dimethicone 3.0 Sodium hyuronate 0.1 Glycine 2.0 Polyacrylamide 5.0 Antioxidant 0.3 Triethanolamine 0.1 EDTA 0.1 Purified water Balance

Claims (8)

A fermented cosmetic composition for promoting damaged skin recovery comprising the yeast fermented extract of hair root and yeast fermented extract of mistletoe as an active ingredient. delete According to claim 1, Fermented cosmetic composition for promoting damaged skin recovery comprising the hair root yeast fermentation extract and mistletoe yeast fermentation extract in a weight ratio of 1 to 10: 1 to 10. According to claim 1, Lactobacillus fermented extract of soybean, further comprising a fermentation cosmetic composition for promoting damaged skin recovery. The fermentation cosmetic composition for promoting damaged skin recovery of claim 4, wherein the hair root yeast fermentation extract, mistletoe yeast fermentation extract and soybean lactobacillus fermentation extract are contained in a weight ratio of 1 to 10: 1 to 10: 1 to 10. The method of claim 1, wherein the yeast is at least one damaged skin repair selected from the group consisting of Saccharomyces cerevisiae, Shigeru caromyces Pombe, Clereromyces lactis and Saccharomyces bolladi Fermentation cosmetic composition for promotion. The method according to claim 4, wherein the Lactobacillus is selected from the group consisting of Lactobacillus brevis, Lactobacillus casei, Lactobacillus plantarum, Lactobacillus ashdophyllus, Lactobacillus helvetus and Lactobacillus delbruechi subspecies Bulgaricus. Fermented cosmetic composition for promoting damaged skin recovery of at least one. According to any one of claims 1, 3 to 5, wherein the cosmetic composition promotes damaged skin recovery, characterized in that formulated as a cream, softening water, astringent makeup, milk lotion, foundation, soap or face wash foam Fermentation cosmetic composition for.

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