KR20080094457A - Anti-wrinkle cosmetic composition - Google Patents

Abstract

An anti-wrinkle cosmetic composition is provided to be safe to skin and show excellent collagen synthesis promoting effect, thereby providing excellent skin wrinkle improving effect and wound healing effect and maintaining the effect excellently. A cosmetic composition for preventing wrinkles comprises 0.0001-10 wt.% of a fermented extract of at least one medicinal stuff selected from the group consisting of Glycyrrhiza glabra, Angelica tenuissima, Agastache rugosa, Platycodon gradiflorum, Cervus nippon, Angelica gigas, Zizyphus jujube, Cordyceps sinensis, Aucklandia lappa, Poria cocos, Angelica dahurica, Amomum villosum, Thymus vulgalis, Punica granatum, Acorus tatarinowii, Tuber melanosporum, Tricholoma matsutake, Ganoderma lucidum, Lindera strichnifolia, Gentiana scabra, ginseng, Paeonia albiflora, Eugenia caryophyllata, Gleditsia sinensis, Citruunshiu, Atractylodes chinensis, Saussurea involucrate, Zanthoxylum piperitum, Prunus armeniaca and red ginseng, wherein the fermented extract is obtained by adding yeast, lactic acid bacteria or bifidobacterium to the medicinal stuff and then culturing it or by inoculating fungi to the medicinal stuff and then solid-fermenting it.

Description

Anti-wrinkle cosmetic composition

The present invention relates to a cosmetic composition for improving wrinkles, and more particularly, to a cosmetic composition for improving wrinkles having excellent collagen synthesis promoting effect, safe for skin, and an excellent effect for improving skin wrinkles and wound healing and sustaining the effects thereof. .

Collagen, a major component of the extracellular matrix, is present in extracellular epilepsy as a major substrate protein produced by skin fibroblasts. It is also an important protein, accounting for about 30% of the total weight of biological proteins, and has a solid triple helix structure. Collagen forms most of the organic matter in the skin, tendons, bones and teeth, especially in bones and skin (dermis). Most other sieve structures exist as fibrous inclusions.

Collagen is a relatively weak immunogen, partly due to the shielding of latent antigenic determinants by the collagen's helix, which also makes collagen resistant to proteolysis. The main functions of collagen are known as mechanical firmness of skin, reluctance of connective tissue and binding of tissue, support of cell adhesion, induction of cell division and differentiation (in organic growth or wound healing) (Van der Rest et al. , Ann NY Acad Sci, 1990).

This collagen is reduced by age and photoaging by UV irradiation, which is known to be closely associated with wrinkle formation of the skin (Arthur K. Balin et al., Aging and the skin, 1989). Collagen also plays an important role in wound healing and promotes the synthesis of collagen in the damaged epithelium, allowing the wound to recover quickly and without scars.

Conventionally, in order to take advantage of collagen's skin moisturizing effect and wound healing effect, products containing collagen in an external cosmetic composition such as cosmetics or ointment have been released. However, these products are collagen, which is a polymer material, applied to the skin surface. Because it is difficult to absorb the percutaneous effect of moisturizing or wound healing, it could not be said that it exhibits essential skin function improvement and wound healing function.

In order to solve this problem, interest in collagen synthesis promoting substances has been increased, and known collagen synthesis promoting substances are vitamin C, retinoic acid, transforming growth factor (TGF) (Cardinale G. 35 al, Adv. Enzymol., 41, p.425, 1974), protein from animal placenta (Japanese Patent Laid-Open No. 8-231370), betulinic acid (Japanese Patent Laid-Open No. 8-208424), Chlorella extract (Japanese Patent Application Laid-open No. Hei 9-40526, Japanese Patent Application Laid-open No. Hei 10-36283, fibroblast proliferation promoting action) and the like.

However, these materials have limited usage due to safety problems such as irritation and redness during skin application, or the effects are insignificant, and thus the effect of improving skin function or wound healing could not be expected. Therefore, there is an urgent need for the development of a new skin external preparation composition that is safer and more effective in vivo than existing external skin preparation compositions.

On the other hand, fermentation is a process of decomposing organic matter using enzymes of microorganisms. Fermentation and decay are carried out by a similar process, but when decomposition produces a substance that is useful in our lives, it is called fermentation. Therefore, fermenting the herbal medicine using microorganisms reduces the toxicity of the herbal medicine, lowers the molecular weight, facilitates digestion, or facilitates transdermal absorption when applied to the skin, thereby improving bioavailability.

In addition, herbal medicines may exhibit pharmacological effects by themselves, but the pharmacological effects may be enhanced through fermentation, or harmful ingredients may be made by the metabolism of microorganisms, thereby exerting new pharmacological effects that were not found in herbal medicines before fermentation.

In order to solve the problems of the prior art as described above, an object of the present invention is to provide a cosmetic composition for wrinkle improvement excellent in the skin wrinkles improvement effect and wound healing effect and excellent durability of the collagen synthesis safe and excellent effect It is done.

Another object of the present invention is to further enhance the pharmacological effect of each medicine through the fermentation to provide a cosmetic composition for improving wrinkles improved collagen synthesis further.

In order to achieve the above object, the present invention is licorice, Gobon, Gwakyang, Gilgyeong, Deer Antler, Angelica ear, Jujube, Cordyceps sinensis, Mokyang, Baekbokyeong, Baekji, Sinsa, muskcho, pomegranate, Seokchangpo, truffles, Songi, ganoderma lucidum, oak Wrinkles, characterized in that it comprises fermented extract of medicinal herbs selected from the group consisting of gentian, ginseng, peony, cloves, sculptural, dermis, creation, cheonsan snow softening, cheonhwabun, bokcho, algae and red ginseng as an active ingredient It provides a cosmetic composition for improvement.

The fermented extract may be obtained by adding and culturing the medicine to yeast, lactic acid bacteria or bifidus bacteria.

The fermented extract can be obtained by inoculating fungi on fungus and solid fermentation.

The herbal fermentation extract is preferably contained in 0.0001 to 10% by weight in the cosmetic composition.

The cosmetic composition may be prepared in the form of external skin ointment, flexible cosmetics, nourishing cosmetics, nutrition cream, massage cream, essence, pack, emulsion, oil gel and the like.

Hereinafter, the cosmetic composition for wrinkle improvement of the present invention will be described in detail.

The medicinal extracts described in the present invention are licorice, Gobon, Gwakyang, Gilgyeong, Deer Antler, Angelica, Jujube, Cordyceps sinensis, Mokyang, Baekbokyeong, Baekji, Cod, Muskcho, Pomegranate, Seokchangpo, Mt. , Ginseng, peony, cloves, sculptor, dermis, creation, cheonsanol softening, cheonhwabun, bokcho, algae and red ginseng extract means obtained by extracting according to a conventional method. In addition, the medicinal herb extract refers to a secondary extract obtained by inoculating and cultivating the medicinal herb extract in yeast, lactic acid bacteria or bifidus bacteria or by solid fermentation by adding fungi to commercially available medicinal herbs or ground products.

The present inventors conducted a study to develop a new collagen synthesis promoting material for animals and plants fermented by microorganisms useful to develop a substance having a collagen synthesis promoting effect at the cellular level using human-derived fibroblasts, licorice , Gobon, Gwakhyang, Gilgyeong, Deer Antler, Angelica, Jujube, Cordyceps sinensis, Mokyang, Baekbokyeong, White paper, Cod, Musk vinegar, Pomegranate, Seokchangpo, Songro mushroom, Pine mushroom, Ganoderma lucidum, Ojak, Gentian, Ginseng, Peony, Clove, Carver, Dermis It has been found that fermentation broths such as creation, cheonsan snow softening, cheonhwabun, bokcho, passerby, red ginseng have an excellent effect of promoting collagen synthesis compared to the respective extracts, and completed the present invention.

The cosmetic composition for improving wrinkles of the present invention is licorice, Gobon, Gwakhyang, Gilgyeong, Deer Antler, Angelica, Jujube, Cordyceps sinensis, Mokyang, Baekbokyeong, White paper, Sign, Musk vinegar, Pomegranate, Seokchangpo, Mt. It contains, as an active ingredient, fermented extract of medicinal herbs selected from the group consisting of ginseng, peony, cloves, sculptural, dermis, creation, cheolsan softening, cheonhwabun, bokcho, almond and red ginseng.

The licorice (Glycyrrhiza glabra) is a perennial herb belonging to the legume (Legumisosae), dried in the shade and used as medicinal or tea, and used for neuralgia, cough, arteriosclerosis, and difficulty urination. Angelica tenuissima is a perennial herb belonging to the umbelliferae and has an effect on abdominal pain, toothache and diarrhea. The Gwaghyang (Agastache rugosa) is an annual herb belonging to the Lamiaceae (Labiatae) is used for gastropathy, sweating, fever, dry stomach. The Gilt (Platycodon gradiflorum) is a perennial herb belonging to the genus Campanulaceae, which is used for post-human pain, toothache and so on. The antler (Cervus nippon) is dried by harvesting the camouflage of the plum or aquatic animal belonging to the family Deeraceae (Cervidae), protect the liver and gods and strengthen bones and muscles. Angelica gigas is a perennial root belonging to the genus Umbelliferae and is used for physical weakness, headache, constipation, and the like. The jujube (Zizyphus jujube) is a fruit of the broad-leaved arborescent in Rhamnaceae, and is used for nourishment, tonic, detoxification, Jinhae, and pain. Cordyceps sinensis belongs to the fungus (Hypocreaceae), and is used for anti-cancer, arteriosclerosis, high blood pressure, heart disease. The `` Aucklandia lappa '' is a root of perennial herb belonging to the compositae, which is used in the quartet and the regional rebellion. The Baekbokryeong (Poria cocos) belongs to the genus Polygonoraceae (Polypooraceae) and is used for gravitational force, sogal, diarrhea. The angelica dahurica is a biennial herb belonging to the family Umbelliferae, and is used for headaches, dizziness, and guanwasa. Amomum villosum is a perennial herb belonging to the family Zingiberaceae and is used for vomiting, diarrhea, and anxiety. The musk vine (Thymus vulgalis) is a perennial herb belonging to the Lamiaceae (Labiatae) is used for headache, depression, anemia, fatigue recovery. The pomegranate (Punica granatum) is a perennial deciduous broad-leaved arborescent belonging to the family Pomegranate (Punicaceae), it is used for atherosclerosis, diarrhea and athlete's foot. The changchangpo (Acorus tatarinowii) belongs to Chunnamseong (Araceae), is used for forgetfulness, purulent tumors, bruises. The truffle (Tuber melanosporum) is used as an edible mushroom of the subterranean fungus of the Aspergillus aureus. The cluster (Tricholoma matsutake) is a fungus of the fungus fungi, blood cholesterol inhibitory effect, blood circulation (hand, foot numbness, lower back and knee symptom treatment), arteriosclerosis, heart disease, diabetes, hyperlipidemia and the like. The Ganoderma lucidum is a fruiting body of Ganoderma lucidum and myoma, belonging to the Polypooraceae family, and is used for atherosclerosis, neuralgia, and cancer. The pentagram (Lindera strichnifolia) is a root of Cheontaepoong belonging to the camphor tree (Lauraceae), and has the effects of cold bladder, urine frequency, white turbidity, and paralysis. The gentian (Gentiana scabra) is a perennial herb belonging to the gentian genus (Gentianaceae) is used for swelling, epilepsy, roundworm, eczema. The ginseng is a perennial root of the genus Ogari (Araliaceae) is used for blood circulation, cold hands and feet, bronchitis and the like. The peony (Paeonia albiflora) is a perennial herb belonging to Ranunculus (Ranunculaceae) is used to promote blood circulation, analgesic, jingyeong. The clove (Eugenia caryophyllata) dried the buds of the clove, belonging to the evergreen tree belonging to the genus Cloves (Myrtaceae), is used for rebellion, bad breath, sexual activation. The sculptural (Gleditsia sinensis) is a dry spiny of the thorn tree belonging to the legume (Reguminosae), it is used for boils, insects and the like. The dermis (Citruunshiu) is a tangerine belonging to the mountain range (Ruraceae) is dried skin of mature fruit. The Atractylodes chinensis is a perennial herb belonging to the compositae and dried tubers of mountain wine, and is used for stroke, conjunctivitis, hypertension, and dizziness. The shunsan (Sussurea involucrate) is a perennial herb belonging to Ranunculus (Ranunculaceae) is used as an analgesic, swelling, cardiac, diuretic and the like. Zanthoxylum piperitum is a mature pericarp of the Sancho tree belonging to the family Ruralaceae, which is used for abdominal pain, diarrhea, antiparasitic action, and skin eczema. Prunus armeniaca is a seed of mature fruit of the apricot tree belonging to the Rosaceae family, and is used for the treatment of coughing and breathing. The red ginseng (Red gienseng) is steamed by steaming ginseng, is used for cardiac action, diabetes, rejuvenation, enhancing sexual function.

The medicinal herbs can be prepared as a medicinal extract according to a conventional method, for example, extracted with an organic solvent, such as distilled water, anhydrous or lower alcohols having 1 to 4 carbon atoms, concentrated under reduced pressure and dried to obtain a primary extract. Can be. Specifically, licorice, gobon, kwahyang, gilkyung, antler, donkey, jujube, cordyceps, mokyang, baekbokyeong, baekji, sine, muskcho, pomegranate, seokchangpo, truffles, pine mushrooms, ganoderma lucidum, oak, yongdam, ginseng , Peony, cloves, sculptural, dermis, creation, cheonseolyeon softening, hwahwahwa, chopsticks, almonds or red ginseng, and finely pulverized, and 5 to 20 volumes of water or 1 to 4 carbon atoms An organic solvent such as a hydrous lower alcohol is heated in an extractor equipped with a reflux condenser for 1 to 5 hours to extract extracts of the medicinal herbs. After filtration with a filter cloth, the residue is further extracted one or more times in the same manner, the extracts are combined and concentrated under reduced pressure, lyophilized or spray-dried to obtain a dry extract.

The medicinal herb extracts obtained as described above may be added to and cultured in yeast, lactic acid bacteria or bifidus bacteria to obtain a final medicinal herb fermentation extract. At this time, the medicinal herb extract is preferably added in 1 to 20% by weight to the basal medium of yeast, lactic acid bacteria or bifidus bacteria. In addition, the medicinal herb fermentation extract may be obtained by inoculating fungi (fungi) and solid fermentation by medicinal herb or fragment, which is commercially available.

The yeast may be used Saccharomyces cerevisiae , Schizosaccharomyces ellipsoids , Saccharomyces boulardii and the like.

Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus bulgaricus, Lactobacillus rhamnosus, Lactobacillus plantarum, Lactobacillus reuteri, Lactobacillus confusus, Lactobacillus fermentum, Lactobacillus brevis and the like can be used.

The bifidus bacteria may be Bifidobacterium breve, Bifidobacterium bifidum, Bifidobacterium longum , Bifidobacterium infantis and the like.

The fungus can be used as a fungus of medicinal mushrooms such as chaga (Inonnotus-obliquus), Cordyceps sinensis, Paecilomyces japonica , Trichoma matsutake, phellinus linteus, Ganoderma lucidum and the like. have.

More specifically, when using yeast as a microorganism, licorice, bonbon, walnut, gilyeong, antler, donkey, jujube, cordyceps sinensis, mokyang, baekbokyeong, white paper, sign at a constant concentration (about 10 ^{5-10 7} cells / mL) , Musk, pomegranate, Seokchangpo, truffles, pine, ganoderma lucidum, gentian, ginseng, peony, cloves, sculptor, dermis, creation, cheonseolyeon, cheonhwabun, bokcho, almond and red ginseng After inoculation of the yeast to the basal medium (YM medium), it is incubated for 30 to 120 hours while maintaining the conditions of pH 5.5 to 6.5, temperature 25 to 35 ° C, and aeration amount of 0.05 to 0.5 VVM. Thereafter, the fermentation broth may be centrifuged to remove the polymer precipitate and the yeast cells, concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling capacitor, and then lyophilized to obtain respective herbal fermentation extracts.

When using the lactic acid bacteria to the microorganism has a constant concentration (about 10 ⁵ ~10 ⁷ cells / mL) in licorice, gobon, gwakhyang, gilgyeong, antler, angelica root, date palm, Cordyceps, elecampane, baekbokryeong, a blank check, sign and four herbs, pomegranate, Basic medium (MRS medium) containing primary extracts of Seokchangpo, truffles, pine, ganoderma, oak, gentian, ginseng, peony, cloves, sculptor, dermis, creation, cheonsan snow softening, cheonhwabun, bokcho, almond and red ginseng After the inoculation of lactic acid bacteria, the cells were incubated for 24 to 120 hours while maintaining the conditions of pH 6.5 to 7.5, temperature of 35 to 38 ° C, and aeration of 1 VVM. Thereafter, the fermentation broth may be centrifuged to remove polymer precipitates and lactic acid bacteria, concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling capacitor, and then lyophilized to obtain respective medicinal fermentation extracts.

When using Bifidus microorganism as a microorganism, licorice, bonbon, jugyang, gilyeong, deer antler, donkey, jujube, cordyceps, moth, baekbokyeong, white paper, sine, musk vine, pomegranate at a constant concentration (about 10 ^{5-10 7} cells / mL) Basic medium containing the primary extracts of Seokchangpo, truffles, pine, ganoderma lucidum, oak, gentian, ginseng, peony, cloves, sculptor, dermis, creation, cheonsan snow softening, cheonhwabun, bokcho, almond and red ginseng ) Inoculated with Bifidobacteria, and incubated for 24 to 120 hours while maintaining the pH of 6.5 ~ 7.5, temperature 35 ~ 38 ℃, anaerobic culture conditions. Thereafter, the fermentation broth may be centrifuged to remove the polymer precipitate and the bifidus cells, concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling capacitor, and then lyophilized to obtain respective herbal fermentation extracts.

In addition, when fungi are used as microorganisms, licorice, bonbon, kwakhyang, gilkyung, antler, donkey, jujube, cordyceps, mokyang, baekbokyeong, baekji, sine, musk vine, pomegranate, seokchangpo, truffle mushroom, pine mushroom, velvet, Inoculate 1-10% by weight of fungus on each of the five powders, gentian, ginseng, peony, cloves, sculptor, dermis, creation, cheonsan snow softening, cheonhwabun, bokcho, almonds and red ginseng itself or slices and powdered ground products. Each fermentation extract of the medicinal herb can be obtained by solid fermentation at -25 ° C and humidity of 60 to 70% for 60 days.

When the medicinal herb fermentation extracts are treated to human-derived fibroblasts, they show significantly superior collagen synthesis promoting effects compared to common medicinal herb extracts. In addition, when the cosmetics are prepared using the medicinal fermentation extracts and applied to human skin, it is safe for the skin without any side effects, and shows very good skin wrinkle improvement and wound healing effect.

Herbal fermentation extract obtained as described above is preferably contained in 0.0001 to 10% by weight, more preferably 0.0005 to 10% by weight in the cosmetic composition for improving wrinkles of the present invention. If the content is less than 0.0001% by weight, no obvious effect can be expected. If the content is more than 10% by weight, there is no evidence of a distinct effect due to the increase in content.

The formulation of the cosmetic composition for improving wrinkles comprising the medicinal fermentation extract of the present invention as an active ingredient is not particularly limited and may be appropriately selected as desired. For example, the cosmetic composition of the present invention may be prepared in the form of external skin ointment, softening longevity, nourishing longevity, nutrition cream, massage cream, essence, pack, emulsion, oil gel and the like. In this case, the skin external ointment may be prepared to contain 50 to 97% by weight of petrolatum and 0.1 to 5% by weight of polyoxyethylene oleyl-ether phosphate in addition to the medicinal fermentation extract of the present invention, the flexible cosmetics fermentation of the present invention In addition to the extract, it may be prepared to contain 1 to 10% by weight of polyhydric alcohols (propylene glycol, glycerin, etc.) and 0.05 to 2% by weight of surfactants (polyethylene oleyl ether, polyoxyethylene hydrogenated castor oil, etc.). In addition, nutrient cosmetics and nourishing cream is 5 to 20% by weight of oils (squalane, petrolatum, octyldodecanol, etc.) and wax components (cetanol, stearyl alcohol, beeswax, etc.) in addition to the medicinal fermentation extract of the present invention It can be prepared to contain%, essence can be prepared by containing 5 to 30% by weight of polyhydric alcohols such as glycerin, propylene glycol in addition to the medicinal fermentation extract of the present invention. Massage cream is prepared by containing 30 to 70% by weight of oil such as liquid paraffin, petrolatum, isononyl isononanoate in addition to the medicinal fermentation extract of the present invention, the pack is polyvinyl alcohol 5-20 in addition to the medicinal fermentation extract of the present invention Wash off containing 5-30% by weight of pigments such as kaolin, talc, zinc oxide and titanium dioxide in addition to the medicinal fermentation extract of the present invention in a peel-off pack or a general emulsion cosmetic containing ) Pack.

In addition, the cosmetic composition for improving wrinkles of the present invention, in addition to the ingredients described above in each formulation, oil, water, surfactants, moisturizers, lower alcohols, thickeners, chelating agents, pigments, preservatives, fragrances, etc. It can be used in combination as needed.

Hereinafter, preferred examples are provided to help understanding of the present invention, but the following examples are merely to illustrate the present invention, and the scope of the present invention is not limited to the following examples.

EXAMPLE

Example 1

Licorice, Gobon, Gwakhyang, Gilgyeong, Deer Antler, Angelica, Jujube, Cordyceps sinensis, Mokyang, Baekbokyeong, White paper, Cod, Muskcho, Pomegranate, Seokchangpo, Songro mushroom, Songi, Ganoderma lucidum, Ojak, Gentian, Ginseng, Peony, Clove, Carver, 200 g of each pulverized powder obtained from dermis, cultivation, cheonsan snow softening, cheonhwa powder, candle, almond and red ginseng were put in purified water or 3 L of 80% methanol, and boiled for 3 hours in reflux extractor with cooling capacitor. It was. Then, the filtrate was filtered through a 100 mesh filter cloth and the residue was extracted once more in the same manner. Each extract was combined and filtered with Whatman No. 2 filter paper at room temperature to remove insoluble materials, concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling capacitor, and then freeze-dried to dry each dry weight as shown in Table 1 below. Got. Table 1 shows the dry weight of the extract per 100 g of medicinal herbs according to the extraction solvent.

Medicine 80% methanol Purified water licorice 8.3 g 7.9 g Book 8.5 g 8.1 g Kwak Hyang 6.3 g 5.8 g Street view 5.3 g 5.1 g velvet 6.6 g 5.4 g Donkey 9.2 g 8.3 g Jujube 10.2 g 9.3 g Cordyceps sinensis 6.6 g 6.1 g elecampane 6.8 g 6.2 g Baikyeongryeong 8.5 g 7.8 g blank 8.3 g 8.1 g sign 7.5 g 7.1 g Musk 4.3 g 4.1 g Pomegranate 6.2 g 5.6 g Seokchangpo 8.3 g 7.3 g Truffles 9.2 g 7.9 g cluster 7.4 g 6.8 g wisdom 8.1 g 7.1 g Oak 7.3 g 7.0 g Gentian 11.5 g 10.1 g Ginseng 6.4 g 6.0 g Peony 7.3 g 6.0 g cloves 5.2 g 4.8 g Engraver 7.5 g 7.3 g dermis 5.6 g 5.3 g Creation 7.6 g 6.5 g Cheonsan Snow 4.8 g 4.3 g Natural flower 7.3 g 6.8 g Scent 8.3 g 8.1 g Passerby 6.5 g 6.1 g Red ginseng 6.3 g 5.9 g

Examples 2 to 32

Each medicinal herb extract obtained using 80% methanol was fermented by adding 5% by weight to YM medium, which is a basic medium for the yeast Saccharomyces cerevisiae.

Specifically, Saccharomyces cerevisiae was inoculated into yeast basal medium (YM medium) containing 5% by weight of each medicinal herb extract at a concentration of about 10 ^{5-10 7} cells / mL, followed by pH 5.5-6.5, temperature 25-35. C was incubated for 48 hours while maintaining the conditions of 0.05-0.5 VVM of airflow. Thereafter, 100 g of each fermentation broth was centrifuged to remove the polymer precipitate and the yeast cells, and then concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling capacitor, followed by lyophilization to obtain a final medicinal yeast fermentation dried product. The dry weight of the medicinal yeast fermented dry matter is shown in Table 2 below.

Medicine Fermentation broth dry weight (g) Medicine Fermentation broth dry weight (g) Example 2 (Liquorice) 5.71 Example 18 (cluster) 6.22 Example 3 (the original) 6.11 Example 19 (geography) 6.18 Example 4 (contour) 6.23 Example 20 (Sleaze) 6.32 Example 5 (length) 6.00 Example 21 (Gentian) 6.12 Example 6 (rust) 6.12 Example 22 (Ginseng) 6.15 Example 7 (Dangui) 5.97 Example 23 Peony 6.13 Example 8 (jujube) 6.11 Example 24 (Cloves) 6.02 Example 9 (Cordyceps sinensis) 6.16 Example 25 (sculptor) 5.93 Example 10 (neck) 6.05 Example 26 (dermis) 5.93 Example 11 (Baekbokyeong) 6.10 Example 27 (creation) 6.00 Example 12 (blank paper) 6.07 Example 28 (Shenshan smelting) 6.11 Example 13 (Sign) 6.08 Example 29 (cloth) 6.22 Example 14 (Silk Candle) 6.10 Example 30 (concentration) 6.15 Example 15 (pomegranate) 6.12 Example 31 (passenger) 5.93 Example 16 (windshield) 6.04 Example 32 (red ginseng) 6.04 Example 17 (Truffle Mushroom) 6.05

Examples 33-63

Each medicinal extract obtained by using 80% methanol was fermented by adding 5% by weight to the MRS medium, which is a basic medium for Lactobacillus acidophilus, a lactic acid bacterium.

Specifically, Lactobacillus acidophilus was inoculated into a lactic acid bacterium base medium (MRS medium) containing 5% by weight of each medicinal herb extract at a concentration of about 10 ^{5-10 7} cells / mL, followed by pH 6.5-7.5, temperature 35-38. C was incubated for 48 hours while maintaining the conditions of 1 VVM of aeration amount. Thereafter, 100 g of each fermentation broth was centrifuged to remove the polymer precipitate and the lactic acid bacteria, and then concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling capacitor, followed by freeze drying to obtain a final medicinal herb lactobacillus dried product. The dry weight of medicinal lactic acid bacteria fermented dry matter is shown in Table 3 below.

Medicine Fermentation broth dry weight (g) Medicine Fermentation broth dry weight (g) Example 33 (Liquorice) 5.73 Example 49 (cluster) 5.89 Example 34 (the original) 6.02 Example 50 (geography) 6.06 Example 35 (contour) 6.16 Example 51 (Sleaze) 5.97 Example 36 (length) 5.92 Example 52 (Gentian) 6.04 Example 37 (rusting) 6.15 Example 53 (Ginseng) 6.09 Example 38 (Dangi) 6.05 Example 54 (Peanut) 6.22 Example 39 (jujube) 6.05 Example 55 (Cloves) 5.91 Example 40 (Cordyceps sinensis) 6.11 Example 56 (sculptor) 6.12 Example 41 (neck) 6.01 Example 57 (dermis) 6.08 Example 42 (Baekbokyeong) 5.99 Example 58 (creation) 6.04 Example 43 (blank paper) 5.91 Example 59 (Shenshan smelting) 6.05 Example 44 (Sign) 5.93 Example 60 (powder) 6.09 Example 45 Musk Candle 6.15 Example 61 (concentration) 6.02 Example 46 (pomegranate) 5.98 Example 62 (passenger) 6.04 Example 47 6.12 Example 63 (red ginseng) 6.19 Example 48 (Truffle Mushroom) 5.99

Examples 64-94

Each medicinal extract obtained using 80% methanol was fermented by adding 5% by weight to BL medium, which is a basic medium for Bifidobacterium longum, which is a bifidus bacterium.

Specifically, Bifidobacterium longum was inoculated in a lactic acid bacteria basic medium (BL medium) containing 5 wt% of each medicinal herb extract at a concentration of about 10 ^{5-10 7} cells / mL, followed by pH 6.5-7.5, temperature 35-38. Incubated for 48 hours while maintaining the anaerobic conditions, ℃. Thereafter, 100 g of each fermentation broth was centrifuged to remove the polymer precipitate and the bifidus cells, and then concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling capacitor, followed by lyophilization to obtain a final medicinal bifidus fermented product. The dry weight of the medicinal bifidus fermented dry matter is shown in Table 4 below.

Medicine Fermentation broth dry weight (g) Medicine Fermentation broth dry weight (g) Example 64 (Liquorice) 5.83 Example 80 (cluster) 6.26 Example 65 (main text) 6.13 Example 81 (geography) 6.01 Example 66 (contour) 6.04 Example 82 (Sleaze) 6.03 Example 67 (length) 6.15 Example 83 (Gentian) 6.14 Example 68 (rusting) 6.02 Example 84 (Ginseng) 6.15 Example 69 (Dangui) 6.11 Example 85 Peony 6.03 Example 70 (jujube) 6.22 Example 86 (Cloves) 5.99 Example 71 (Central cordyceps) 6.13 Example 87 (sculptor) 6.16 Example 72 (neck) 6.00 Example 88 (dermis) 6.20 Example 73 (Backpacking age) 5.91 Example 89 (creation) 6.17 Example 74 (blank paper) 6.13 Example 90 (Shenshan Smelt) 6.13 Example 75 (sign) 6.04 Example 91 (cloth) 6.26 Example 76 (scented candle) 6.13 Example 92 (tactility) 6.13 Example 77 (pomegranate) 6.02 Example 93 (passenger) 6.19 Example 78 6.04 Example 94 (red ginseng) 6.11 Example 79 (Truffle Mushroom) 6.04

Examples 32-41

The medicine used in Table 1 was sectioned and fungi (fungi) was added to 5% by weight of solid fermentation.

Specifically, each 200 g of the medicinal herb as a slice inoculated Paecilomyces japonica at 5% by weight under constant humidity, fermented and dried for 60 days at 18-25 ℃, 60-70% humidity, and then pulverized 100 Each g was put into 3L of 80% methanol, and extracted by boiling for 3 hours in a reflux extractor with a cooling capacitor. Then, the filtrate was filtered with 100 mesh filter cloth and the residue was extracted once more in the same manner. Each extract was combined and filtered with Whatman No. 2 filter paper at room temperature to remove insoluble matters, and then concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling condenser and then lyophilized to obtain a final medicine mold fermentation dried product. The dry weight of the medicinal fungus fermented dry matter is shown in Table 5 below.

Medicine Fermentation broth dry weight (g) Medicine Fermentation broth dry weight (g) Example 95 (Liquorice) 7.52 Example 111 (cluster) 4.80 Example 96 (the original) 7.83 Example 112 (geography) 6.77 Example 97 (contour) 5.87 Example 113 (Sleaze) 6.62 Example 98 (length) 4.92 Example 114 (Gentian) 9.02 Example 99 (rust) 5.32 Example 115 (Ginseng) 5.43 Example 100 (Dangui) 8.02 Example 116 (Peanut) 5.12 Example 101 (jujube) 8.86 Example 117 (clove) 4.11 Example 102 (Cordyceps sinensis) 5.81 Example 118 (sculptor) 6.83 Example 103 (neck) 5.67 Example 119 (dermis) 4.70 Example 104 (Backpacking age) 7.11 Example 120 (creation) 5.81 Example 105 (blank paper) 7.64 Example 121 (Tianshan Smelt) 3.87 Example 106 (Sign) 6.64 Example 122 (powder) 6.23 Example 107 musk candles 4.07 Example 123 (the candle) 7.63 Example 108 (pomegranate) 5.03 Example 124 (passenger) 5.56 Example 109 (stone) 6.78 Example 125 (red ginseng) 5.61 Example 110 (Truffle Mushroom) 6.80

Experimental Example 1. Collagen synthesis promoting effect

Each of the medicinal herb extracts obtained using 80% methanol in Example 1 and the fermented medicinal herb extracts of Examples 2 to 110 were added to the culture solution of human-derived fibroblasts to test collagen synthesis effects at the cellular level. The synthesis of collagen was quantified using a PICP EIA kit (Procollagen Type I C-Peptide Enzyme ImmunoAssay KIT).

The fermented medicinal herb extracts of Examples 2 to 110 were added to each culture medium of human fibroblast cells so as to have a final concentration of 0.05%, and cultured for 1 day. The degree of collagen synthesis was measured at 450 nm using a spectrophotometer. At this time, the degree of collagen synthesis was measured by the same method for the culture medium (control) of the fibroblasts to which nothing was added and the sample added with vitamin C to a final concentration of 0.005%.

Collagen production amount was measured by UV absorbance, the collagen production increase rate was calculated as the ratio of the collagen production relative to the control group and the results are summarized in Tables 6 to 10 below.

sample Collagen Production % Increase Control group (no addition) 10118 ± 0.030 - YM medium (final concentration 0.005%) 1.202 ± 0.0032 107.51 MRS medium (final concentration 0.005%) 1.215 ± 0.041 108.68 BL medium (final concentration 0.005%) 1.223 ± 0.041 109.39  Licorice (final concentration 0.005%) Extract of Example 1 1.332 ± 0.060 119.14 Yeast Fermented Extract of Example 2 1.628 ± 0.061 145.62 Lactic Acid Bacteria Fermented Extract of Example 33 1.654 ± 0.048 147.94 Bifidus fermented extract of Example 64 1.687 ± 0.063 150.89 Mold Fermentation Extract of Example 95 1.762 ± 0.065 157.60  Hard bones (final concentration 0.005%) Extract of Example 1 1.312 ± 0.021 117.35 Yeast Fermented Extract of Example 3 1.612 ± 0.092 144.19 Lactic Acid Bacteria Fermented Extract of Example 34 1.543 ± 0.072 138.01 Bifidus fermented extract of Example 65 1.599 ± 0.081 143.02 Mold Fermentation Extract of Example 96 1.634 ± 0.077 146.15  Kwak Hyang (final concentration 0.005%) Extract of Example 1 1.235 ± 0.052 110.47 Yeast Fermented Extract of Example 4 1.534 ± 0.098 137.21 Lactic Acid Bacteria Fermented Extract of Example 35 1.611 ± 0.030 144.10 Bifidus fermented extract of Example 66 1.523 ± 0.082 136.23 Mold Fermentation Extract of Example 97 1.674 ± 0.046 149.73

sample Collagen Production % Increase  Gilkyung (final concentration 0.005%) Extract of Example 1 1.356 ± 0.048 121.29 Yeast Fermented Extract of Example 5 1.660 ± 0.058 147.48 Lactic Acid Bacteria Fermented Extract of Example 36 1.624 ± 0.056 145.26 Bifidus fermented extract of Example 67 1.629 ± 0.072 145.71 Mold Fermentation Extract of Example 98 1.691 ± 0.083 151.25  Deer Antler (final concentration 0.005%) Extract of Example 1 1.272 ± 0.034 113.77 Yeast Fermented Extract of Example 6 1.557 ± 0.069 139.27 Lactic Acid Bacteria Fermented Extract of Example 37 1.502 ± 0.068 134.35 Bifidus fermented extract of Example 68 1.673 ± 0.034 149.64 Mold Fermentation Extract of Example 99 1.772 ± 0.071 158.50  Angelica (final concentration 0.005%) Extract of Example 1 1.242 ± 0.067 110.91 Yeast Fermented Extract of Example 7 1.575 ± 0.067 140.88 Lactic Acid Bacteria Fermented Extract of Example 38 1.536 ± 0.056 137.39 Bifidus fermented extract of Example 69 1.600 ± 0.056 143.11 Mold Fermentation Extract of Example 100 1.563 ± 0.062 139.98  Jujube (final concentration 0.005%) Extract of Example 1 1.357 ± 0.056 121.38 Yeast Fermented Extract of Example 8 1.768 ± 0.045 158.14 Lactic Acid Bacteria Fermented Extract of Example 39 1.623 ± 0.056 145.17 Bifidus fermented extract of Example 70 1.656 ± 0.066 148.12 Mold Fermentation Extract of Example 101 1.699 ± 0.074 151.97  Cordyceps sinensis (final concentration 0.005%) Extract of Example 1 1.267 ± 0.024 113.33 Yeast Fermented Extract of Example 9 1.652 ± 0.076 147.58 Lactic Acid Bacteria Fermented Extract of Example 40 1.584 ± 0.050 141.68 Bifidus fermented extract of Example 71 1.641 ± 0.039 146.78 Mold Fermentation Extract of Example 102 1.777 ± 0.058 158.94  Mokhyang (final concentration 0.005%) Extract of Example 1 1.299 ± 0.034 116.19 Yeast Fermented Extract of Example 10 1.622 ± 0.080 145.08 Lactic Acid Bacteria Fermented Extract of Example 41 1.755 ± 0.045 156.98 Bifidus fermented extract of Example 72 1.512 ± 0.065 135.24 Mold Fermentation Extract of Example 103 1.740 ± 0.033 155.64  Baekbokyeong (final concentration 0.005%) Extract of Example 1 1.223 ± 0.098 109.40 Yeast Fermented Extract of Example 11 1.595 ± 0.054 142.67 Lactic Acid Bacteria Fermented Extract of Example 42 1.566 ± 0.097 139.18 Bifidus fermented extract of Example 73 1.656 ± 0.077 148.12 Mold Fermentation Extract of Example 104 1.672 ± 0.065 149.55  White paper (final concentration 0.005%) Extract of Example 1 1.345 ± 0.078 120.30 Yeast Fermented Extract of Example 12 1.736 ± 0.078 157.42 Lactic Acid Bacteria Fermented Extract of Example 43 1.867 ± 0.045 166.99 Bifidus fermented extract of Example 74 1.811 ± 0.090 161.99 Mold Fermentation Extract of Example 105 1.653 ± 0.047 147.85  Sine (final concentration 0.005%) Extract of Example 1 1.324 ± 0.053 118.42 Yeast Fermented Extract of Example 13 1.744 ± 0.055 155.99 Lactic Acid Bacteria Fermented Extract of Example 44 1.623 ± 0.056 145.17 Bifidus fermented extract of Example 75 1.624 ± 0.064 145.26 Mold Fermentation Extract of Example 106 1.598 ± 0.070 142.93

sample Collagen Production % Increase  Musk vinegar (final concentration 0.005%) Extract of Example 1 1.412 ± 0.054 126.30 Yeast Fermented Extract of Example 14 1.767 ± 0.033 158.05 Lactic Acid Bacteria Fermented Extract of Example 45 1.762 ± 0.056 157.87 Bifidus fermented extract of Example 76 1.658 ± 0.077 148.30 Mold Fermentation Extract of Example 107 1.866 ± 0.045 166.91  Pomegranate (final concentration 0.005%) Extract of Example 1 1.315 ± 0.088 117.62 Yeast Fermented Extract of Example 15 1.752 ± 0.099 156.71 Lactic Acid Bacteria Fermented Extract of Example 46 1.655 ± 0.077 148.03 Bifidus fermented extract of Example 77 1.687 ± 0.051 150.89 Mold Fermentation Extract of Example 108 1.701 ± 0.086 52.15  Seokchangpo (final concentration 0.005%) Extract of Example 1 1.284 ± 0.081 114.85 Yeast Fermented Extract of Example 16 1.556 ± 0.033 139.18 Lactic Acid Bacteria Fermented Extract of Example 47 1.634 ± 0.056 146.15 Bifidus fermented extract of Example 78 1.589 ± 0.056 142.13 Mold Fermentation Extract of Example 109 1.611 ± 0.056 144.10  Truffle Mushroom (final concentration 0.005%) Extract of Example 1 1.332 ± 0.038 119.14 Yeast Fermented Extract of Example 17 1.655 ± 0.066 148.03 Lactic Acid Bacteria Fermented Extract of Example 48 1.756 ± 0.088 157.07 Bifidus fermented extract of Example 79 1.702 ± 0.065 152.24 Mold Fermentation Extract of Example 110 1.658 ± 0.078 148.30  Pine (Final Concentration 0.005%) Extract of Example 1 1.351 ± 0.048 120.84 Yeast Fermented Extract of Example 18 1.702 ± 0.057 152.24 Lactic Acid Bacteria Fermented Extract of Example 49 1.767 ± 0.055 158.05 Bifidus bacterium fermentation extract of Example 80 1.788 ± 0.073 159.93 Mold Fermentation Extract of Example 111 1.677 ± 0.055 150.00  Ganoderma lucidum (final concentration 0.005%) Extract of Example 1 1.323 ± 0.076 118.34 Yeast Fermented Extract of Example 19 1.632 ± 0.056 145.97 Lactic Acid Bacteria Fermented Extract of Example 50 1.600 ± 0.051 143.11 Bifidus fermented extract of Example 81 1.534 ± 0.067 137.21 Mold Fermentation Extract of Example 112 1.712 ± 0.063 153.13  Ointment (final concentration 0.005%) Extract of Example 1 1.345 ± 0.077 120.30 Yeast Fermented Extract of Example 20 1.699 ± 0.032 146.81 Lactic Acid Bacteria Fermented Extract of Example 51 1.633 ± 0.039 146.81 Bifidus fermented extract of Example 82 1.775 ± 0.076 158.77 Mold Fermentation Extract of Example 113 1.711 ± 0.052 153.04  Gentian (Final Concentration 0.005%) Extract of Example 1 1.266 ± 0.048 113.24 Yeast Fermented Extract of Example 21 1.655 ± 0.087 148.12 Lactic Acid Bacteria Fermented Extract of Example 52 1.613 ± 0.092 144.28 Bifidus fermented extract of Example 83 1.587 ± 0.045 141.95 Mold Fermentation Extract of Example 114 1.564 ± 0.060 139.89  Ginseng (final concentration 0.005%) Extract of Example 1 1.345 ± 0.045 120.30 Yeast Fermented Extract of Example 22 1.744 ± 0.056 155.99 Lactic Acid Bacteria Fermented Extract of Example 53 1.652 ± 0.076 147.76 Bifidus fermented extract of Example 84 1.828 ± 0.067 168.16 Mold Fermentation Extract of Example 115 1.678 ± 0.055 150.09

sample Collagen Production % Increase  Peony (final concentration 0.005%) Extract of Example 1 1.343 ± 0.045 120.13 Yeast Fermented Extract of Example 23 1.656 ± 0.081 148.12 Lactic Acid Bacteria Fermented Extract of Example 54 1.612 ± 0.070 144.19 Bifidus fermented extract of Example 85 1.711 ± 0.042 153.04 Mold Fermentation Extract of Example 116 1.563 ± 0.054 139.80  Cloves (final concentration 0.005%) Extract of Example 1 1.343 ± 0.044 120.12 Yeast Fermented Extract of Example 24 1.655 ± 0.056 148.12 Lactic Acid Bacteria Fermented Extract of Example 55 1.743 ± 0.044 155.90 Bifidus fermented extract of Example 86 1.656 ± 0.050 148.12 Mold Fermentation Extract of Example 117 1.899 ± 0.072 169.86  Engraver (final concentration 0.005%) Extract of Example 1 1.242 ± 0.033 111.09 Yeast Fermented Extract of Example 25 1.610 ± 0.023 144.01 Lactic Acid Bacteria Fermented Extract of Example 56 1.755 ± 0.045 156.98 Bifidus fermented extract of Example 87 1.692 ± 0.056 151.34 Mold Fermentation Extract of Example 118 1.501 ± 0.072 134.26  Dermis (final concentration 0.005%) Extract of Example 1 1.377 ± 0.044 123.17 Yeast Fermented Extract of Example 26 1.711 ± 0.023 153.04 Lactic Acid Bacteria Fermented Extract of Example 57 1.632 ± 0.079 145.97 Bifidus fermented extract of Example 88 1.877 ± 0.072 167.89 Mold Fermentation Extract of Example 119 1.603 ± 0.073 143.38  Creation (final concentration 0.005%) Extract of Example 1 1.260 ± 0.055 112.70 Yeast Fermented Extract of Example 27 1.623 ± 0.079 145.17 Lactic Acid Bacteria Fermented Extract of Example 58 1.545 ± 0.067 138.01 Bifidus fermented extract of Example 89 1.688 ± 0.088 150.98 Mold Fermentation Extract of Example 120 1.597 ± 0.053 142.84  Tianshan Sulfation (final concentration 0.005%) Extract of Example 1 1.322 ± 0.080 118.25 Yeast Fermented Extract of Example 28 1.599 ± 0.055 143.02 Lactic Acid Bacteria Fermented Extract of Example 59 1.536 ± 0.067 137.39 Bifidus bacterium fermentation extract of Example 90 1.730 ± 0.048 155.60 Mold Fermentation Extract of Example 121 1.677 ± 0.067 150.00  Cheonhwa (final concentration 0.005%) Extract of Example 1 1.356 ± 0.093 121.29 Yeast Fermented Extract of Example 29 1.644 ± 0.052 147.05 Lactic Acid Bacteria Fermented Extract of Example 60 1.722 ± 0.044 154.02 Bifidus fermented extract of Example 91 1.681 ± 0.082 150.36 Mold Fermentation Extract of Example 122 1.634 ± 0.067 146.15  Tactile (Final Concentration 0.005%) Extract of Example 1 1.233 ± 0.056 110.29 Yeast Fermented Extract of Example 30 1.675 ± 0.033 149.82 Lactic Acid Bacteria Fermented Extract of Example 61 1.588 ± 0.052 142.04 Bifidus fermented extract of Example 92 1.544 ± 0.056 138.10 Mold Fermentation Extract of Example 123 1.602 ± 0.056 143.29  Alpine (final concentration 0.005%) Extract of Example 1 1.354 ± 0.071 121.11 Yeast Fermented Extract of Example 31 1.762 ± 0.077 157.60 Lactic Acid Bacteria Fermented Extract of Example 62 1.556 ± 0.056 139.18 Bifidus fermented extract of Example 93 1.665 ± 0.056 148.93 Mold Fermentation Extract of Example 124 1.766 ± 0.073 157.96

sample Collagen Production % Increase Red ginseng (final concentration 0.005%) Extract of Example 1 1.353 ± 0.066 121.02 Yeast Fermented Extract of Example 32 1.755 ± 0.052 156.98 Lactic Acid Bacteria Fermented Extract of Example 63 1.658 ± 0.066 148.30 Bifidus fermented extract of Example 94 1.813 ± 0.052 162.16 Mold Fermentation Extract of Example 125 1.772 ± 0.064 158.50 Vitamin C (final concentration 0.0005%) 1.528 ± 0.075 136.67

As shown in Tables 6 to 10, each of the medicinal herb fermentation extracts obtained according to Examples 2 to 125 significantly increased collagen production in human-derived fibroblasts compared to the extract using 80% methanol in Example 1, Considering that it is not purified at all, it can be seen that there is a very strong collagen synthesis promoting ability in fibroblasts compared with vitamin C, a known collagen synthesis promoter.

Experimental Example  2. Collagen synthesis promoting effect according to concentration

For the human-derived fibroblasts cultured in the same manner as in Experimental Example 1 by adjusting the concentration of the licorice fungus fermented extract of Example 95 to 0.5%, 0.05%, 0.005%, 0.0005% respectively collagen synthesis The promoting ability was investigated and the results are shown in Table 11 below.

sample Application concentration (%) Melanin production Inhibition Rate (%) Control group (no addition) - 1.118 ± 0.030 - Licorice Fungus Ferment Extract of Example 95 Final concentration (0.0005%) 1.502 ± 0.043 134.35 Final concentration (0.005%) 1.762 ± 0.065 157.60 Final concentration (0.05%) 1.888 ± 0.078 168.87 Final concentration (0.5%) 1.923 ± 0.051 135.94 Vitamin c Final concentration (0.0001%) 1.528 ± 0.075 172.00

As shown in Table 11, it was confirmed that the licorice fungi fermentation extract has a very strong collagen synthesis promoting ability against cultured human-derived fibroblasts. Although the licorice fungus fermented extract is not a single substance but a mixture, it has the same or higher collagen synthesis promoting ability at the same concentration as the vitamin C. As it has strong collagen synthesis promoting ability, it could be expected to be very useful as a skin wrinkle improver or wound healing agent.

Example 126 and Comparative Example 1

Using the licorice fungus fermented extract of Example 95, an external skin ointment was prepared according to the prescription of Table 12 below.

division Example 126 Comparative Example 1 Licorice Fungus Ferment Extract 10 - Diethyl sebacate 8 8 Prepayment 5 5 Polyoxyethylene oleyl ether phosphate 6 6 Sodium benzoate Quantity Quantity vaseline To 100 To 100

Example 127 and Comparative Example 2

Using the licorice fungus fermented extract of Example 95, a cream was prepared according to the prescription of Table 13.

division Example 127 Comparative Example 2 Licorice Fungus Ferment Extract 1.0 - Stearic acid 15.0 15.0 Cetanol 1.0 1.0 Potassium hydroxide 0.7 0.7 glycerin 5.0 5.0 Propylene glycol 3.0 3.0 antiseptic Quantity Quantity incense Quantity Quantity Purified water To 100 To 100

Example 128 and Comparative Example 3

Using the licorice fungus fermented extract of Example 95 was prepared by the softener according to the formula of Table 14.

division Example 128 Comparative Example 3 Licorice Fungus Ferment Extract 0.2 - ethanol 10.0 10.0 Polylauric acid polyoxyethylene sorbitan 1.0 1.0 Methyl paraoxybenzoate 0.2 0.2 glycerin 5.0 5.0 1,3-butylene glycol 6.0 6.0 incense Quantity Quantity Pigment Quantity Quantity Purified water To 100 To 100

Example 129 and Comparative Example 4

Using the licorice fungus fermented extract of Example 95 was prepared nutritional cosmetics by the prescription of Table 15 below.

division Example 129 Comparative Example 4 Licorice Fungus Ferment Extract 0.1 - Waselin 2.0 2.0 Sesquioleate sorbitan 0.8 0.8 Polyoxyethylene oleylethyl 1.2 1.2 Methyl paraoxybenzoate Quantity Quantity Propylene glycol 5.0 5.0 ethanol 3.2 3.2 Carboxy Vinyl Polymer 18.0 18.0 Potassium hydroxide 0.1 0.1 Pigment Quantity Quantity incense Quantity Quantity Purified water To 100 To 100

Example 130 and Comparative Example 5

Using the licorice fungus fermented extract of Example 95, a pack was prepared according to the prescription of Table 16 below.

division Example 130 Comparative Example 5 Licorice Fungus Ferment Extract 0.5 - glycerin 5.0 5.0 Propylene glycol 4.0 4.0 Polyvinyl alcohol 15.0 15.0 ethanol 8.0 8.0 Polyoxyethylene oleylethyl 1.0 1.0 Methyl paraoxybenzoate 0.2 0.2 incense Quantity Quantity Pigment Quantity Quantity Purified water To 100 To 100

Example 131 and Comparative Example 6

Essence was prepared by the formulation of Table 17 using the licorice fungus fermented extract of Example 95.

division Example 131 Comparative Example 6 Licorice Fungus Ferment Extract 5.0 - Propylene glycol 10.0 10.0 glycerin 10.0 10.0 Sodium hyaluronate aqueous solution (1%) 5.0 5.0 ethanol 5.0 5.0 Polyoxyethylene Cured Castor Oil 1.0 1.0 Methyl paraoxybenzoate 0.1 0.1 incense Quantity Quantity Purified water To 100 To 100

Experimental Example 3. Effect of wound healing by collagen synthesis

The effects of wound healing were tested using a tensile strength method that reflects the quality and amount of wound regeneration in the incision in a 5 week old male rat.

First, the hair on the back of the rat was removed, and then cut with a scalpel and the incision was closed. The creams of Example 127 and Comparative Example 2 were administered to the incision site once daily for 6 days. After 6 days of administration, the rats used for the experiment were slaughtered, the skin of the wound site was extracted, three specimens of width 1 cm orthogonal to the iron wire were made for each individual, and the tearing force was measured by a rheometer. The measured tensile strength (g / cm) was used as an index of the strength of the regenerated collagen fibers. The increase rate of tensile strength was expressed as relative strength with 100% of the tensile strength of the control group to which only ethanol was applied without adding a sample. The strength measurement results are summarized in Table 18 below.

division Tensile Strength (g / cm) % Increase Example 127 563 100 Comparative Example 2 659 117

Experimental Example 4. Wrinkle improvement effect

Using the cream of Example 127 and Comparative Example 2 was carried out a panel test for the skin wrinkle improvement effect.

First, 30 healthy women from 35 to 50 years old were divided into two groups of 15 people, group 1 applied the cream of Comparative Example 2, and group 2 applied the cream of Example 127 to the facial part once a day for 3 months. It was made. After 3 months, the degree of improvement of skin wrinkles was evaluated through a subject survey. The skin wrinkle improvement and elasticity of the test subjects were determined in three stages of no improvement, slight improvement, and significant improvement compared to before use, and the results are shown in Table 19 below.

division No improvement Minor improvements A significant improvement Example 127 One 7 7 Comparative Example 2 8 6 One

As shown in Table 19, when the cream of Example 127 according to the present invention was used, it was found that the skin wrinkle improvement and elasticity enhancing effect were excellent.

The present invention described above is not limited to the above-described embodiments, and various substitutions, modifications, and changes can be made without departing from the technical spirit of the present invention. It is obvious to those who have it.

The cosmetic composition for improving wrinkles according to the present invention is safer on the skin and further enhances the pharmacological effect of each medicinal agent through fermentation, effectively promoting collagen synthesis, and having an excellent skin wrinkle improving effect and wound healing effect and sustainability of the effect.

Claims (11)

Licorice, Gobon, Gwakhyang, Gilgyeong, Deer Antler, Angelica, Jujube, Cordyceps sinensis, Mokyang, Baekbokyeong, White paper, Cod, Muskcho, Pomegranate, Seokchangpo, Songro mushroom, Pine mushrooms, Ganoderma lucidum, Ojak, Yongdam, Ginseng, Peony, Clove, Carver, Wrinkle improvement cosmetic composition comprising as an active ingredient fermented extract of at least one selected from the group consisting of dermis, creation, cheonseolyeon softening, cheonhwabun, bokcho, almond and red ginseng. The method of claim 1, The fermentation extract is wrinkles cosmetic composition for improving wrinkles, characterized in that obtained by culturing, yeast, lactic acid bacteria or bifidus bacteria. The method of claim 2, The fermentation extract is wrinkles cosmetic composition for improving wrinkles, characterized in that added to the base medium of yeast, lactic acid bacteria or bifidus bacteria in 1 to 20% by weight. The method of claim 2, The yeast is wrinkles cosmetic composition for improving wrinkles, characterized in that at least one selected from the group consisting of Saccharomyces cerevisiae , Schizosaccharomyces ellipsoids and Saccharomyces boulardii . The method of claim 2, Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus bulgaricus, Lactobacillus rhamnosus, Lactobacillus plantarum, Lactobacillus reuteri and Lactobacillus confusus, Lactobacillus fermentum, Lactobacillus brevis cosmetics selected from the group consisting of a cosmetic composition selected from the group consisting of dogs . The method of claim 2, Bifidobacterium bacteria Bifidobacterium breve, Bifidobacterium bifidum, Bifidobacterium longum and Bifidobacterium infantis is selected from the group consisting of cosmetic composition for improving wrinkles. The method of claim 1, The fermentation extract is a cosmetic composition for improving wrinkles, characterized in that obtained by inoculating fungi (fungi) in the medicine and solid fermentation. The method of claim 7, wherein The fungus is wrinkle improvement, characterized in that at least one selected from the group consisting of chaga (Inonnotus-obliquus), cordyceps fungi (Cordyceps sinensis, Paecilomyces japonica ), mushrooms (phellinus linteus) and Ganoderma lucidum Cosmetic composition for. The method of claim 1, The pharmaceutical fermentation extract is a cosmetic composition for improving wrinkles, characterized in that contained in the cosmetic composition 0.0001 to 10% by weight. The method of claim 1, The cosmetic composition is a cosmetic composition for improving wrinkles, characterized in that the skin external ointment, flexible cosmetics, nourishing cosmetics, nutrition cream, massage cream, essence, pack, emulsion, or oil gel formulation. The method of claim 1, The cosmetic composition for wrinkle improvement further comprises an additive selected from the group consisting of oil, water, surfactants, moisturizers, lower alcohols, thickeners, chelating agents, pigments, preservatives, and fragrances. .