KR101951295B1 - Skin external composition comprising fermented extracts of pruni cortex and antler and preparation method thereof - Google Patents

Abstract

The present invention relates to a composition for external application for skin, comprising fermented extracts of Prunus sargentii and deer antler, and a method for producing the same. More particularly, the present invention relates to a composition for external application containing extracts whose content of active ingredients is increased by using fermented microorganisms in Prunus sargentii and deer antler, and a method for producing a composition using these extracts.

Description

FIELD OF THE INVENTION [0001] The present invention relates to a skin external composition comprising fermented extracts of ferns and antler, and a skin external composition comprising fermented extracts of ferulic extracts and antler and preparation methods thereof,

The present invention relates to a composition for external application for skin, which comprises a fermented extract of horsetail and antler, and more particularly to a composition for external application for skin, which comprises extracting the composition so as to increase the content of the active ingredient using a fermenting microorganism, Compositions and methods of making compositions using these extracts.

Generally, human body skin protects the human body from external environmental stimuli such as temperature, humidity change, ultraviolet ray, fine dust, and pollutants. It is degraded by external physical, chemical stimulation, stress and nutritional deficiency. And causes skin aging phenomena such as elasticity deterioration, keratinization, and wrinkle formation.

In order to prevent the skin aging phenomenon as described above and to maintain a healthy skin and elastic skin, the physiologically active substance obtained from various copper, plants, microorganisms and the like is added and mixed cosmetics are used to maintain the natural function of the skin, Researches and developments are underway to effectively inhibit skin aging.

In order to inhibit the aging of the skin, new natural substances have been developed and used. However, these substances have insufficient whitening effect, and safety against skin has been limited due to formulation problems and safety problems in formulation into cosmetics It is true.

In addition, when the composition is used as an external preparation, particularly a cosmetic composition, the composition is not absorbed and it is difficult to achieve a desired skin improvement effect. Thus, various methods of delivery have been studied, and studies using drug carriers such as liposomes are actively under way.

Liposome is an artificially produced vesicle composed of a lipid bilayer, and has a structure similar to that of a vital cell membrane. The liposomes are biocompatible and composed of a phospholipid, which is a major component of a vital cell membrane, and exist in the form of capsules in which a hydrophilic and hydrophobic substance in an aqueous solution is surrounded by a lipid bilayer of liposome. Such liposomes are capable of storing desired useful substances (drugs, nutrients, etc.) in the bilayer structure, and are capable of transporting unstable and difficult-to-handle substances into cells. However, in spite of these advantages, the liposome has a problem that not only the formulation is unstable, the collection efficiency is very low, but also the solvent and preservative used for liposome production cause skin irritation. In addition, the cholesterol used as a raw material of the liposome has a problem that it forms a liposome lipid membrane firmly and hinders the release of the trapping material and lowers the cell membrane permeability of the trapping material.

The present inventors aim to provide a new skin external drug extract material which can safely extract natural products and maximize skin elasticity and improvement effects.

Accordingly, the present inventors intend to provide a novel method for preparing a composition for external application containing a natural extract as an active ingredient.

In order to attain the above object, the present invention provides a composition for external application for skin comprising a fermented extract of fern and antler.

The present invention also relates to a process for the production of fermented beverages comprising: (a) fermenting a mixture of fennel and antler;

(b) extracting the active ingredient from the fermentation product with a solvent; And

(c) collecting the extract with liposomes.

It was confirmed that the antioxidant activity and nutrition according to the present invention were supplied to improve skin elasticity and wrinkle. The composition of the present invention is derived from a natural product and can be used safely as a cosmetic composition having no side effects and having an anti-aging activity.

1 shows photographs before and after application of the composition of the present invention to Experiment 1 in Experimental Example 5 ((A), (B): before application, (C), (D)
2 shows photographs before and after application of the composition of the present invention to Experiment 2 in Experimental Example 5 ((A), (B): before application, (C), (D)

Hereinafter, the present invention will be described in detail.

The present invention provides a composition for external application for skin comprising a fermented extract of horsetail and deer antler.

Peony (Prunus sargentiiRehder) is a bark of a mountain cherry tree. It grows up to 20 meters tall with a stem of a broad-leaved large-leaved tree with rose leaves. There is a lot of branches in the middle of the stem, and it spreads obliquely to the side, and the upper part becomes a conical shape like a broom turned upside down. Inhabits in forests, valleys, and beaches of highland 1,600m or less in mountains. It can be seen mainly in the 600m high mountain. The skin of the stem has long horizontal bark like stripes. Between April and May, blossom in pink or white, with young leaves in the leaf running spot, or slightly before the leaves. Two or three flower stalks are gathered, and like the umbrella fern, flowers with a diameter of about 2.5 ~ 4㎝ run on the end. There are five petals. Calyx leaf is divided into 5 branches and is reddish brown. There are many surgeries. There is no scent. Leaves 8 ~ 12㎝ long are hanging on branches. It has an oval shape with a sharp tip and a sharp saw blade-like sawtooth on the edge. When it is young, it has reddish and sticky hairs, and its front side becomes dark green gradually. The back side is white. The petiole is reddish, hairless, and has a pair of reddish-blue nymphs on its upper side. It is yellow in autumn, and dyes in red. Round fruit with seeds sealed with a solid nucleus in May to June is yellowish red to blackish brown and shiny. Young trees are dark reddish brown. It is smooth and there are many peel eyes that are long side. As you stay, the color becomes brown or dark gray, with light gray stains and peeled eyes peeled off to the side. When it becomes a dead wood, its skin divides like shallow scales. The edges are bright yellow. Inside there is a wide core of yellowish brown. In the middle, there is a small yellowish brown color. The branches are reddish purple and gradually reddish and light brown. When you stay, it is purplish and dark gray and has peeled eyes. It is thick and it is a little bit hard. It is a conical shape with a narrow bottom and a pointed end. It is reddish or brownish. Medicinal use: bronchitis, cystitis, enteritis, insomnia Edible: fruit stem skin, twig (uff, 櫻 皮) Take it in spring and dry it in the windy shade. Bronze, cystitis, intestinal inflammation 5g dried in water to 700 ml, dalyeoseo drink. Fruit (Yaotong, 櫻 草 花) It is collected in spring ~ summer and dries in the shade where wind is well. 5 g of insomnia dried in water 700 ml, then drink dalyeoseo. The raw cherries are eaten as fruits. It is a deciduous broad-leaved tree that grows in the mountains. The stem divides into several branches from a relatively low position and strikes many branches. Leaves are placed alternately and have an egg length of 5-8cm. The base of the leaf is rounded, the tip is pointed, and small sawtooth is evenly arranged on the edge. The young leaves are reddish and have fine hairs on both sides. The flowers bloom with 2-3 leaves with leaf at the end of short branch and have long peduncle with more than 4cm. The color of the flower, about 3.5cm in diameter, is pale pink. After the flowers are bloomed, they are reddish as they grow in round fruit with a diameter of about 6mm and turn black. It is widely distributed throughout the country and lies in the forests of the mountain forests. The fur (桜 peel). It is also called 火 皮. I use the shell of eggplant as medicine. Cherry blossoms, cherry blossoms, cherry blossoms, and some cherry blossoms. Collect during the growing season and dry in the sun. Peel off the rough parts and cut into small pieces. Theylkinine, Coumarin, Phlorrhizin, Amygdalin, Malic acid and the like are contained. Jinhae, has the efficacy of detoxification. Applicable diseases are cough, urticaria (dermatitis), dermatitis and itching. To stop coughing, take 4 to 8 grams of dried medicines at a time with 200 cc of water, or take the medicinal product with a powder.

The effective ingredient of the herb improves skin diseases such as various dermatitis and acne, and acts to make the skin moist and elastic.

Deer antler is dried after cutting deer horns of deer and plum blossoms, 丸花, 大 鹿, and deer hairs in dense, unbroken or slightly ossified young horns. A deer is usually born and can cut its horn in about three years. The strength of the horn grows so strong that it leads to all the pharmacists. Therefore, the tip of the horn is the best medicine.

The main ingredients of antler are various free amino acids and polysaccharides, glycosaminoglycans (GAGs), hyaluronic acid, keratin, sialic acid, cholesterol, fatty acids, phospholipids , Inorganic components, and the like. As for the distribution of the active ingredients of each part of the antler, the content of collagen, ash, calcium and other mineral components, which are indicators of the chemical composition, increases as the area of the antler reaches the lower part of the antler, and the content of the protein, fat, glycosaminoglycan, , Uronic acid and the like are decreased.

It can be classified into three types of antler antler in Cervusnippon Temminck, Cervuselaphus Linne and Cervuscanadensis Erxleben. have. The young horn, which is not yet ossified or slightly ossified, is called antler, and the one that is already ossified is called the 鹿角. This clay is made by boiling in water and is made into a thickened collagen mass.

When you look at the antlers from the tip to the tail, divide them into four parts: antler, tip, upper section, mid section, and base. According to this standard, the antler of the antler and the antler of the antler belong to the categories of antler, the antler, and the antler.

The antler pieces will be thinned by removing the hair from the antler by a suitable method. The characteristics of the antler are as thin as 1 ~ 3mm in thickness and dried. The outer surface of the cut surface is yellowish brown or dark brown. The inside of the cut surface is pale yellow, reddish brown or grayish white or reddish brown, and is easily broken. The weight is light, the touch is relatively hard, there are many fine holes, and the spongy shape is formed and there is a peculiar smell.

① When using the antler as a medicine, remove the hair on the surface of the dried antler, remove the hair with a glass knife or lightly burn it and scour it with a glass knife.

② Cut the antler into a suitable size and let the yellow juice leave it or let it pour a little alcohol.

③ When lightly steamed, cut thinly and then dried, it is called antler.

According to the processing method of the antler, it is called antler (deer antler) in which the antler of the antler is removed in the first precession, and antler antler (powder of antler antler) (证准 繩), milk juice was processed into an emulsion antler (制 制 茸 茸 茸) and in the case of 方 方 (博 方) processed by using alcohol was called a main antler .

The active ingredients of horny and deer antioxidant, antimicrobial activity and anti-inflammatory action increase skin's shine and elasticity and maintain healthy skin.

The present invention provides a composition for external application for skin comprising a fermented extract of a mixture of an epidermis and an antler as an active ingredient. Preferably, the mixing ratio thereof is 3: 1 in terms of dry weight. In addition to these, one kind of pharmacological agent selected from the group consisting of a lover, Shiho, Dansam, Dandelion, Angelica gigas, Citrus peony, Hwanggi, Baekjang and Baiji can be added.

The present invention also relates to a process for the production of fermented beverages comprising: (a) fermenting a mixture of fennel and antler;

(b) extracting the active ingredient from the fermentation product with a solvent; And

(c) collecting the extract with liposomes.

The step (a) is a step of fermenting the mixture of the fern and the antler according to the present invention, which uses a fermenting microorganism selected from the group consisting of lactic acid bacteria, Bacillus subtilis, yeast, and mixtures thereof, And it is possible to maximize the pharmacological effect by allowing the active ingredient to be contained quickly and uniformly without loss. The fermentation can be obtained by fermenting for 1 to 6 days while maintaining the pH at 5 to 7 under a temperature condition of 20 to 50 ° C, preferably fermenting at pH 6 for 5 days at 40 ° C. If the fermentation is less than 1 day, sufficient fermentation will not occur, and if it is more than 6 days, there is a fear of corruption. In one example, fermentation can be performed with lactic acid bacteria. The lactic acid bacteria may be selected from the group consisting of Streptococcus spp., Lactococcus spp., Enterococcus spp., Lactobacillus spp., Pediococcus spp., Leuconostoc spp., Weissella spp. ) And Bifidobacterium genus, but is not limited thereto, and more preferably, it is Lactobacillus acidophilus.

To increase the enzyme reaction in the fermented product, sugar and microorganisms may be mixed. The mixing ratio of the fermented product and the microorganism is preferably 1: 0.5 to 2 based on the weight ratio, It is preferably 1 to 10% by weight, more preferably 3 to 5% by weight, based on the total weight of the tea fermentation product and sugar. In addition, in order to increase the efficiency of the fermentation process, the fermentation is performed after the first fermentation and sterilization, and then the second fermentation is performed under the same condition as the first fermentation in the state where the second sugar and the microorganism are mixed, The content of the component can be increased.

In the step (b) of the present invention, the fermentation product is extracted, and the active ingredient is extracted from the fermentation product, and the extract may be prepared using a conventional extraction method. That is, a method may be employed in which an extraction solvent is added to the above fermentation product and extraction is carried out at a low temperature or a high pressure under a high temperature. As the extraction solvent, for example, water (or purified water), a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent obtained by mixing them can be used. There is no particular limitation on the amount of the solvent to be used, but the amount of the solvent may be about 1 to 50 times the dry weight of the drug substance to be extracted, considering the extraction efficiency, the efficacy or economical efficiency of the composition, and the like.

In one embodiment, the composition of the present invention may be prepared by extracting using a suitable solvent and then mixing it with a suitable solvent, such as herbal and antler, wherein each medicament may be ground or chopped with a grinder prior to extraction, The antler extract can be extracted together, and the extracts can be prepared in a mixed form from the time of extraction.

In one embodiment, the extract is selected from the group consisting of water (v / w) by weight of dry weight, anhydrous or lower alcohol of 1-4 carbon atoms, propylene glycol, butylene glycol, glycerin, acetone, ethyl acetate, Is prepared by extracting an extraction solvent selected from the group consisting of chloroform, butyl acetate, diethyl ether, dichloromethane, hexane and mixtures thereof at 10 to 100 ° C for 2 to 5 hours, 15 times the weight of the solvent is added, and the mixture is extracted at 90 DEG C for 3 hours.

The solvent of the present invention is preferably selected from the group consisting of water, an anhydrous or lower alcohols having 1-4 carbon atoms, propylene glycol, butylene glycol, glycerin, acetone, ethyl acetate, chloroform, butyl acetate, diethyl ether, dichloromethane, hexane, , More preferably extracted with butyl acetate, diethyl ether, dichloromethane, hexane or water, and most preferably extracted with diethyl ether.

Needless to say, it is also possible to use the powder after the powder is prepared by an additional process such as vacuum distillation, freeze-drying, or spray drying.

In one embodiment, the external preparation is a composition for external application for anti-aging which improves skin elasticity and wrinkles. More specifically, it promotes collagen production, thereby achieving skin elasticity and wrinkle-reducing effect.

The extract of the present invention may be contained in an amount of 0.00001 to 10% by weight, more preferably 0.001 to 5% by weight, based on the total weight of the external preparation composition. If the content of the flour 11 is less than 0.001% by weight, The effect of skin elasticity by the skin can not be obtained largely. If it exceeds 5% by weight, the increase of the effect is not so large compared with the increase of the content, which is rather inefficient.

In addition, step (c) of the present invention includes collecting the extract prepared in the above step with liposomes. The extract liposome is composed of ceramide / cholesterol / phospholipid complex, which is similar in structure and composition to skin structure, by using a substance constituting phospholipid, which is an important point for skin transfer to the skin, , Ceramide and cholesterol give skin moisturizing effect and Vitamin-E Acetate, an antioxidant, helps skin antioxidant.

The capturing material according to the present invention is a substance to be captured in the lipid bilayer of the liposome containing the emulsifier and the solvent, and is any fermentation extract selected from the group consisting of peel, deer antler and mixtures thereof.

Emulsifiers contain phospholipids and fatty acids, which constitute a lipid bilayer. Examples of the phospholipid include phosphatidylcholine, lysophosphatidylcholine, phosphatidylethanolamine, hydrogenated phophatidylcholine (derived from soybean), phosphatidylserine, phosphatidylglycerol, and phosphatidylinositol ) May be used. Hydrogenerated phosphatidylcholine, which is preferably excellent in oxidation stability, may be used.

The fatty acid may be at least one selected from the group consisting of palmitic acid, stearic acid, oleic acid, linoleic acid and linolenic acid (derived from soybean) .

In addition to the emulsifier, a solvent may be used, and the solvent is such that the lipid bilayer of the emulsifier is in the form of a capsule surrounding the capture material. As the solvent, at least one selected from the group consisting of distilled water, natural butylene glycol, propanediol, glycerin and fermented alcohol can be used. Preferably, distilled water, glycerin can be used, and more preferably distilled water can be used.

The emulsifier may be 70 to 90% by weight of the phospholipid and 1 to 5% by weight of the fatty acid based on the weight of the total emulsifier. The content of such an emulsifier is preferably 0.1 to 10% by weight based on the weight of the whole extract-containing liposome. It is preferable that the content of the solvent is the balance excluding the emulsifier and the additional components based on the weight of the natural liposome containing the fermentation extract such as whole horns.

In addition to the emulsifier, one or more kinds of fatty acids, a coating agent, and an antiseptic may be further included. When the additional substance is added, the content thereof may be 0.1 to 5% by weight based on the weight of the emulsifier.

It is preferable that the liposome containing the fermentation extract such as the husk contains 0.1 to 10% by weight of the emulsifier based on the total weight, 0.1 to 30% by weight of the fermented extract of the present invention, 0.001 to 3% by weight of the preservative, .

The method of collecting liposomes is to uniformly produce liposomes using high energy such as a high pressure type microfluidizer or an ultrasonic wave shredder. However, the present invention is not limited to such a production method, and the steps of each process may be modified or optionally mixed as necessary.

1) dissolving the lipid stabilizing portion and the hydrophilic activating material in a range of from 45 ° C to 50 ° C in the range of 70 ° C to 80 ° C and dissolving the lipid thickening portion and the lipophilic active material portion in the range of 45 ° C to 50 ° C, 2) Adding the lipophilic active material part to the lipid stabilizing part and stirring the mixture at a rotation speed of 1,000 to 2,000 rpm with an agitator to uniformly mix the lipophilic active material part; 3) adding a hydrophilic active material part to the mixture, and 4) forming liposomes by emulsification three to four times using a high-pressure emulsifier.

Preferably, the fermentation extract of the present invention is prepared by liposomes by an ultrasonic method. For example, 1) mixing an emulsifier with a solvent and then subjecting the mixture to an ultrasonic treatment at 35 to 80 ° C, 2) adding a fermented extract of the present invention to the mixture of the first step, and then ultrasonically treating the mixture at 35 to 80 ° C , And 3) repeatedly performing ultrasonic treatment while gradually lowering the temperature after the second step. More preferably, the method may further include a step of adding a coating agent or an antiseptic to the mixture after the step 2), followed by ultrasonic treatment at 35 to 80 ° C.

The ultrasonic treatment is a work for homogenizing a mixture of a natural emulsifier and a solvent, and an ultrasonic liquid processor may be used. As the emulsifier, one or more emulsifiers including a phospholipid and a fatty acid may be used. Examples of the solvent include distilled water, natural butylene glycol, propanediol, glycerin, fermented alcohol, and the like. In step 1, the emulsifier and distilled water are mixed, and the mixture is heated to 35 to 80 ° C, preferably 40 to 70 ° C, and then subjected to ultrasonic treatment to homogenize the phospholipid and fatty acid of the natural emulsifier in the distilled water. In the second step, the fermented extract of the present invention is added to the mixture of the first step and sonicated at 35 to 80 ° C.

The extract can be extracted at room temperature or by heating under the condition that the active ingredient is not destroyed. The extract may be in admixture with an extraction solvent. When distilled water is used as the extraction solvent, the mixing ratio of the fermentation extract such as hulls and distilled water is 0.1: 5 to 1: 1, preferably 1: 1, on the basis of the weight ratio.

The mixture of the fermentation extract such as horsetail and the first step is mixed and then heated to 35 to 80 ° C, preferably 40 to 60 ° C, and then subjected to ultrasonic treatment to reduce the size of the extract and the liposome .

In step 3, the liposome is subjected to repeated ultrasonic treatment while gradually lowering the temperature after the second step, and the liposome is preferably heated at 35 to 70 DEG C for 1 to 10 minutes, preferably 3 to 8 minutes To produce a smaller and more uniform final liposome of the particles formed in the fourth step. The ultrasonic treatment may be repeatedly performed to produce a liposome having a desired particle size, and preferably one to three times.

As described above, the liposome containing the fermentation extract of the present invention is in the form of a capsule surrounded by a lipid bilayer that collects the extract, and can easily deliver the extract into cells. When the liposome is delivered to the cells, the lipid bilayer and the cell membrane easily bind to each other to increase the cell uptake of the extract, thereby maximizing the skin improvement effect such as the collagen production effect.

The composition of the present invention can also be used in a composition for external application for skin for improving skin diseases. In one embodiment, the composition is any one selected from the group consisting of formulations of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray and a powder.

A compound or composition which is an already known and used composition which can be contained in cosmetic preparations as an acceptable carrier in cosmetic preparations, or which is a compound or composition to be developed in the future, which may contain no toxicity, instability or irritability as far as the human body is applicable on contact with the skin have. The carrier may be included in the cosmetic composition of the present invention in an amount of about 1% by weight to about 99.99% by weight, preferably 0.01 to 30% by weight, based on the total weight of the cosmetic composition. However, since the ratio depends on the formulation described above in which the cosmetic composition of the present invention is prepared and on its specific application site (face, neck, etc.) or its desirable application amount, And should not be construed as limiting the scope of the invention.

The composition for external application for skin includes lotion, nutritional lotion, nutrition essence, massage cream, cosmetic bath additive, body lotion, body milk, bath oil, baby oil, baby powder, shower gel, shower cream, sunscreen lotion, sunscreen cream, Skin cream, hand lotion, hair lotion, cosmetic cream, jasmine oil, bath soap, skin lotion, skin cream, sunscreen cosmetics, cleansing milk, , Soap, soap, shampoo, hand cleanser, medicinal soap {non-medical use}, cream soap, facial wash, hair rinse, cosmetic soap, tooth whitening gel, toothpaste and the like. To this end, the composition of the present invention may further comprise an appropriate carrier, excipient or diluent conventionally used in the production of cosmetics.

The carrier, excipient or diluent which may further be added to the composition for external application for skin of the present invention include purified water, oil, wax, fatty acid, fatty acid alcohol, fatty acid ester, surfactant, humectant, thickener, antioxidant, Chelating agents, buffering agents, lower alcohols, and the like. Further, if necessary, it may contain a whitening agent, a moisturizing agent, a vitamin, an ultraviolet screening agent, a perfume, a dye, an antibiotic, an antibacterial agent, and an antifungal agent.

Examples of the carrier include alcohols, oils, surfactants, fatty acids, silicone oils, wetting agents, moisturizers, viscosifying agents, emulsifiers, stabilizers, sunscreens, coloring agents and flavoring agents.

As the oil, hydrogenated vegetable oil, castor oil, cottonseed oil, olive oil, palm oil, jojoba oil and avocado oil may be used. Examples of the wax include wax, wax, carnauba, candelilla, montan, ceresin, liquid paraffin, Can be used.

As the fatty acid, stearic acid, linoleic acid, linolenic acid and oleic acid may be used. As the fatty acid alcohol, cetyl alcohol, octyldodecanol, oleyl alcohol, panthenol, lanolin alcohol, stearyl alcohol and hexadecanol may be used As fatty acid esters, isopropyl myristate, isopropyl palmitate, and butyl stearate may be used. As the surfactant, a cationic surfactant, an anionic surfactant and a nonionic surfactant known in the art can be used, and a surfactant derived from a natural material is preferably used.

In addition, it may contain a hygroscopic agent, a thickening agent, an antioxidant and the like widely known in the field of cosmetics, and the kind and amount thereof are well known in the art.

The composition of the present invention can be identified as a soap composition in another specific embodiment.

When the composition of the present invention is recognized as a soap composition, the soap composition of the present invention can be prepared by incorporating a centipede extract and a coriander extract into a soap base, and further comprises a skin moisturizer, an emulsifier, a water softener, .

Examples of the soap base material include vegetable oils such as palm oil, palm oil, soybean oil, pharmacopoeial olive oil and palm kernel oil or animal fats such as tallow, lard, sunshine and fish oil. Examples of the skin moisturizers include glycerin, erythritol, polyethylene glycol, Propylene glycol, hexylene glycol, isopropyl myristate, silicone derivatives, aloe vera, sorbitol and the like can be used. Examples of the emulsifier include natural oils, wax fatty alcohols, hydrocarbons, natural plant extracts and the like As the water softening agent, tetrasodium ethylenediate etc. may be used.

The soap composition of the present invention may further include an antibacterial agent, a dispersant, a foam inhibitor, a solvent, an inhibitor in water, a corrosion inhibitor, a perfume, a dye, a sequestering agent, an antioxidant, an antiseptic and the like as an additive.

In the soap composition of the present invention, the soap base and additives may be included in amounts commonly used in the art, and the soap base generally comprises 99.999% by weight to 50% by weight based on the total weight of the soap composition And the additive may be added in an amount of 1 wt% to 20 wt%.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are merely illustrative of the present invention and that the scope of the present invention is not limited thereto.

Husk  And fermentation extract of antler

The herbs and antler used in the present invention generally used medicinal materials circulated in the market. They were mixed in a dry weight of 3: 1, washed thoroughly, dried and then pulverized and finely ground using 300 mesh. This was added to a mixture of Lactobacillus acidophilus strains to a mixture of 100 g / L crushed herb and antler. 10 g of glucose was added thereto as a carbon source. The cultures were incubated for 5 days at a temperature of 40 ° C and a pH of 6 using a 5 L fermenter. After culturing, the culture was centrifuged to remove the cultures first. The fermented product was sterilized at 120 ° C and then cultured under the same conditions. The solids were removed from the secondary fermentation and then transferred to the extraction step.

Diethyl ether was added to the fermentation extract, and the mixture was refluxed three times for 5 hours. After cooling, the mixture was filtered through Whatman # 2 filter paper. When the filtration is completed, the extract obtained in the extracting step is transferred to the concentration tank, concentrated under reduced pressure, and lyophilized. Concentration under reduced pressure was performed using a rotary vacuum concentrator. Concentration conditions were as follows: 500 ml of the extract was placed in a 1,000 ml round-bottomed flask, and the mixture was concentrated under reduced pressure while rotating at 1000 rpm under a pressure of 700 mmHg.

Collecting Fermented Extracts Liposomal  Produce

The fermented extracts of fern and antler prepared in Example 1 were prepared as liposomes according to the composition shown in Table 1 below.

First, the emulsifier (Lipoid s75, Lipoid GMBH) was mixed with distilled water, heated to 65 ° C and crushed using an ultrasonic liquid processor. The extract and the distilled water were mixed at a weight ratio of 1: 1 to prepare a collecting material. When the emulsifying agent was homogenized, the prepared collecting material was added, followed by ultrasonic disruption and mixing. When the natural emulsifier and the capturing material were completely homogenized, maltodextrin was added and the homogenization was performed by ultrasonic disintegration. The temperature was gradually lowered and finally ultrasonic disruption was repeated three times for 2 minutes and 30 seconds to homogenize to prepare a liposome containing fermented extract of flax and antler.

ingredient Content (% by weight) Emulsifier Lipoid s75 2 Distilled water Distilled water 92 Coating agent Maltodextrin One extract Fermented extracts of fennel and antler 5

[Comparative Example]

Comparative Example 1: Preparation of fermented extract of hulls

In Example 1, a fermented extract was prepared using only hulls instead of the mixture of horn and antler. All other conditions are the same.

Comparative Example 2: Preparation of fermented extract of antler

In Example 1, a fermented extract was prepared using only antler instead of the mixture of peony and antler. All other conditions are the same.

Comparative Example 3: Preparation of extracts of horn and antler

In Example 1, the extract was directly prepared with diethyl ether without going through the fermentation process of the horseradish and the antler. All other conditions are the same.

[Manufacturing Example]

Production Example 1: Nutritional lotion of the liposome composition of Example 2

The nutritional lotion (Milk Skin Lotion) of Table 2 was prepared using the extract composition prepared in the above example.

ingredient Content (% by weight)  Purified water (Until it reaches 100) glycerin 8.0 Butylene glycol 4.0 Hyaluronic acid extract 5.0 Beta Glucan 7.0 Carbomer 0.1 The liposome composition for collecting the extract of Example 2 3 Caprylic capric triglyceride 8.0 Squalane 5.0 Cetearyl glucoside 1.5 Sorbitan stearate 0.4 Cetearyl alcohol 1.0 antiseptic Suitable amount incense Suitable amount Pigment Suitable amount Triethanolamine 0.1

Production Example 2: Nutritional lotion of the liposome composition collecting the extract of Comparative Example 3

In Example 2, the extract of Comparative Example 3 was used instead of the fermented extract of Example 1 to collect liposomes, and then a nutritional lotion was prepared according to the composition of Preparation Example 1.

Preparation Example 3: Preparation of nutritional cream of liposome composition to collect extract of Example 2

The nutritional creams of Table 3 were prepared using the extract compositions prepared in the above Examples.

ingredient Content (% by weight) Purified water (Until it reaches 100) glycerin 3.0 Butylene glycol 3.0 Liquid paraffin 7.0 Beta Glucan 7.0 Carbomer 0.1 The liposome for capturing the extract of Example 2 3 Caprylic capric triglyceride 3.0 Squalane 5.0 Cetearyl glucoside 1.5 Sorbitan stearate 0.4 Polysorbate 60 1.2 antiseptic Suitable amount incense Suitable amount Pigment Suitable amount Triethanolamine 0.1

Preparation Example 4: Preparation of a massage cream of a liposome composition to collect the extract of Example 2

The massage compositions of Table 4 were prepared using the extract compositions prepared in the above Examples.

ingredient Content (% by weight) Purified water (Until it reaches 100) glycerin 8.0 Butylene glycol 4.0 Hyaluronic acid extract 45.0 Beta Glucan 7.0 Carbomer 0.1 The liposome for capturing the extract of Example 2 3 Caprylic capric triglyceride 3.0 Wax 4.0 Cetearyl glucoside 1.5 Sesquioleic acid sorbitan 0.9 Vaseline 3.0 antiseptic Suitable amount incense Suitable amount Pigment Suitable amount paraffin 1.5

[Experimental Example]

Experimental Example 1: Skin antimicrobial activity test

Staphylococcus epidermidis (KCTC 1917), Staphylococcus aureus (KCTC 3881) and Pseudomonas aeruginosa (KCTC 2004) were used for the antimicrobial activity experiments. Microbial strains used in the antimicrobial experiments were prepared at 37 ℃ in nutrient agar and broth medium Lt; / RTI > To confirm the antimicrobial activity, each strain was assayed by discdiffusion assay. The plate culture medium for antimicrobial test was prepared by laminating 100 μl each of the subcultured strains using a sterilized cotton swab. The sample was slowly absorbed into paper disc (diameter 6 mm) to 2.0 mg per disc, followed by drying to volatilize the solvent After incubation at 37 ° C for 24 hours, the clear zone (mm) produced around the disc was measured and the antimicrobial activity was compared. Gentamicin (BD, USA) 0.01 mg was used as a control. As shown in Table 5 below, it was confirmed that the strain has antibacterial activity against three strains. Of course, the activity was significantly lower than that of gentamycin used as a control, but Example 1 showed the highest activity as compared to gentamycin.

mg / disc Staphylococcus epidermidis Staphylococcus aureus Pseudomonas aeruginosa Example 1 2 8.54 6.87 9.14 Comparative Example 1 2 6.2 5.1 7.8 Comparative Example 2 2 4.7 4.2 5.2 Comparative Example 3 2 3.5 3.9 3.4 Gentamycin 0.01 18.65 20.14 17.36

Experimental Example 2: Antioxidant activity test

1,1-diphenyl-2-picrylhydrazyl (DPPH) (Sigma Co., USA) was used to compare the antioxidative power of Example 1 and Comparative Examples 1 to 3 (Jeong SM et al., J. Korean Soc. Food Sci. Nutr. 33: 1580-1583 (2004)). 100 μl of 0.2 mM DPPH solution was mixed well with 100 μl of each of the fermented extract of fern and deer antler of Example 1-1 and the extracts of Comparative Examples 1 to 3 prepared at a concentration of 100 and 200 μg / ml, followed by reaction at room temperature for 25 minutes , And the absorbance was measured at 517 nm using an ELISA reader (BioTek, USA). The radical scavenging activity of each sample was calculated by the electron donating ability according to the following equation, and the experimental value was expressed as a percentage of the electron donating ability of ascorbic acid used as the positive control group at 100%.

[Mathematical Expression]

Electron donating ability (%) = (1-absorbance of sample addition group / absorbance of no addition group) X 100

Sample electron donating ability (% positive control group) The positive control (100 [mu] g / ml) 100 Example 1 87 Comparative Example 1 64 Comparative Example 2 74 Comparative Example 3 69

As can be seen from Table 6, the fermented extracts of flax and antler exhibited the highest activities compared to the fermented extracts or the fermented extracts, respectively.

Experimental Example 3: Increasing effect of skin elasticity

The skin elasticity improving effects of the above-mentioned Production Examples 1 and 2 were evaluated as follows.

In the experiment (15-20 years old female), the nutrient water prepared in Preparation Example 1 was applied to the right side of the face and the nutrient solution of Production Example 2 was applied to the left side of the face for 2 consecutive months. The skin elasticity was measured by using a skin elasticity meter (SEM 575, C + K Electronic Co., Germany) before and after using the product for 2 months. The experimental results are shown in Table 8 below as the ΔR8 value of Cutometer SEM 575, where the value of R8 indicates the viscoelasticity of the skin.

As shown in Table 7, it was found that the skin elasticity improvement effect of the user who applied the nutrient solution containing the fermented extract of fur and antler was the best in comparison with the extract without the fermentation process (Table 7).

Before use after use Production Example 1 0.13 0.45 Production Example 2 0.12 0.28

Experimental Example 4: Safety test for human skin

To verify the skin safety of the cosmetic compositions of Preparation Example 1, cumulative human skin stimulation tests were conducted. Twenty healthy adults were evaluated in the upper forearm after 1 hour, 12 hours, and 24 hours. The application method is Finn chamber or a similar type of chamber. After applying 15 조성물 of composition per chamber, apply the patch and measure the degree of reaction on the skin every time. The results are shown in Table 8 below. At this time, the skin condition change was visually observed, and the reaction was classified and evaluated according to the following skin condition evaluation criteria

<Change of skin condition standard>

0: No change

1: Very little change

2: slightly changed

3: A little bit harder

4: Severely changed

5: Extremely changed

Test substance Subject response Subject (sex, skin type) Subject 1 (female, neutral) Subject 2 (female, neutral) Subject 3 (female, dry) Time lapse 1 hours 12 hours 24 hours 1 hours 12 hours 24 hours 1 hours 12 hours 24 hours Production Example 1 0 0 0 0 0 0 0 0 0 Production Example 2 0 0 0 0 0 0 0 0 0

From the results of Table 9, it can be seen that almost all the compositions showed no specific reaction, and that the composition of the present invention was safe and less irritating to the skin.

Experimental Example 5: Increase in elasticity in clinical use

In order to see the effect of increasing the elasticity of the cosmetic compositions of Preparation Example 1, the subjects 1 and 2 of the test group of Experimental Example 3 were applied to the face twice a day for three months, respectively, and then the images were compared before and after. 1 (a) and 2 (b), and FIGS. 2 (a) and 2 (b) (c) and 2 (d), respectively. As shown in the figure, the administration of the composition of the present invention shows that the elasticity of the composition of the present invention is improved enough to be visually confirmed.

While the embodiments of the present invention have been described in detail, the scope of the present invention is not limited thereto. Particularly, the drawings show typical treatment examples. As a matter of course, It is easy to know that a composition comprising an acaricide and a globe as an essential ingredient in the invention has excellent effects in the improvement and treatment of various skin diseases.

It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit and scope of the invention as defined in the appended claims.

Claims (12)

delete delete a) A culture solution containing the culture for fermentation was added to a mixture in which the peel and antler antioxidants were mixed in a composition of 3: 1 in terms of dry weight, and the mixture was fermented at 40 DEG C and pH 6 for 5 days. The fermented culture was centrifuged The fermented product is sterilized at 120 ° C., and the fermented product is further fermented at 40 ° C. and pH 6 for 5 days by adding the same culture medium.
b) extracting the active ingredient from the fermentation product with a solvent; And
and c) collecting the extract with liposomes. delete delete [Claim 5] The method according to claim 3, wherein the fermentation comprises at least one strain selected from the group consisting of lactic acid bacteria, Bacillus subtilis bacteria, yeast bacteria, and mixtures thereof. 4. The method according to claim 3, wherein the fermentation is carried out in a culture medium selected from the group consisting of Streptococcus sp., Lactococcus sp., Enterococcus sp., Lactobacillus sp., Pediococcus sp., Leuconostoc sp. Wherein said strain is one or more strains selected from the group consisting of genus Weissella and genus Bifidobacterium. 4. The method according to claim 3, wherein the extraction in step b) is carried out in the presence of water, anhydrous or lower alcohols having 1-4 carbon atoms, propylene glycol, butylene glycol, glycerin, acetone, ethyl acetate, chloroform, butyl acetate, diethyl ether, Is extracted with a solvent selected from the group consisting of methanol, hexane, and mixtures thereof. The method according to claim 3, wherein the step of collecting liposomes in step c) comprises mixing the emulsifier with a solvent and ultrasonically treating the mixture at 35 to 80 캜. 10. The method of claim 9, wherein the emulsifier comprises a phospholipid and a fatty acid. Examples of the phospholipid include phosphatidylcholine, lysophosphatidylcholine, phosphatidylethanolamine, hydrogenated phophatidylcholine, phosphatidylserine, Phosphatidylglycerol and phosphatidylinositol, and the fatty acids include palmitic acid, stearic acid, oleic acid, linoleic acid, linoleic acid, and linolenic acid are used. A composition for external application comprising a liposome containing a fermented extract prepared by the method according to any one of claims 3 and 6 to 10. A cosmetic composition comprising a liposome containing a fermented extract prepared by the method of any one of claims 3 and 6 to 10.

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