KR101855210B1 - Cosmetic composition containing eggplant seed ultrasonicating extract of fermented by aureobasidium pullulans - Google Patents

Abstract

The present invention relates to a cosmetic composition for skin protection obtained by fermentation of black yeast obtained by fermentation of ganoderma lucidum extract obtained from a black yeast fermented ginseng supernatant extract obtained by using an ultrasonic extractor, The present invention is characterized by containing a berry seed extract having improved moisturizing effect and antioxidative effect. INDUSTRIAL APPLICABILITY According to the present invention, it has excellent antioxidative effect, skin moisturizing effect and skin barrier improving effect, and has excellent stability without discoloration, separation and precipitation with respect to temperature change.

Description

TECHNICAL FIELD The present invention relates to a cosmetic composition containing a black yeast fermented eggplant sonicated extract,

The present invention relates to a skin moisturizing and antioxidant cosmetic composition, and more particularly, to a cosmetic composition containing a black yeast fermented egg sonic extract having a moisturizing effect and an antioxidative activity as an active ingredient.

The goal of cosmetics is to maintain beautiful and healthy skin, and also to delay the aging of naturally occurring skin as it gets older. In general, aging is caused by oxidative damage of biomaterials by free radicals. That is, reactive radicals such as active oxygen such as hydrogen peroxide, hydroxy radical, and superoxide radical peroxidize the lipid constituting the cell membrane to destroy the cell membrane, oxidize the protein, attack the nucleic acid and cause oxidative damage, Destroys and eventually kills cells. Skin characteristics that are most noticeable due to aging include wrinkles, decreased elasticity, and skin dullness. In addition to wrinkles and elasticity decline, after 40s, facial color is not clear and is often gray and dark. Especially for young women, when they experience childbirth, they have eosinophilia, blood circulation disorder, which causes their metabolic ability to fall and their skin to become rough and rough. When the metabolism ability is lowered and the skin becomes rough, the water content is also easily reduced. Generally, the moisture content of the skin is about 70% in the dermal layer, but decreases to the skin layer to about 10 to 30% in the keratin layer. The keratinocyte layer is known to have an important role in the moisturization of the skin due to the existence of a substance having a hydrophilic water retention capacity called a natural moisturizing factor (NMF). Normal keratinocyte layer maintains skin smoothness and softness and maintains normal body protection function when moisture is maintained at about 10 to 30%.

Therefore, proper maintenance of the moisture content of the keratinocyte layer is very important for preventing skin aging. However, other natural moisturizing factors such as sodium pyrrolidonecarboxylate (PCA-Na), sodium lactate, There is a problem that the emulsion stability of the cosmetic is impaired due to the strong electrolyte. Amino acids, collagen, elastin, etc. are also capable of moisturizing, but also had a limited ability to moisturize. The ability of the horny layer to retain moisture can be controlled by natural moisturizing factor (NMF), which consists of sebum components, amino acids, lactic acid, urea, and inorganic salts. And the development of a substance with high moisturizing effect is one of the important research subjects in cosmetics. Materials known to have antioxidant activity include ascorbic acid, dl-alpha-tocopherol, beta-carotene, dibutylhydroxyltoluene (BHT), and the like. Since the materials are used as a synthetic material, there is an urgent need to develop new antioxidative cosmetics which can be applied to skin safely and usefully, because they may cause problems of skin safety when used for a long time or in a large amount.

Therefore, studies on skin aging and moisturizing improvement are expected to be continuously carried out. Many cosmetic products using natural products are being developed in order to solve such skin problems and to reduce skin irritation caused by various chemicals. Natural materials are not only less harmful to the skin, but also have increased their value as a raw material for cosmetics due to the recent popularity of cosmetics using natural materials.

For this purpose, researches are being actively carried out to develop materials having excellent safety and skin beauty effect by searching skin physiological activity of various natural vegetable persons.

One branch of natural materials is perennial plant in the temperate zone and perennial plant in the tropics. It is native to India and cultivated from tropical to temperate.

The height is 60-100cm, and the whole plant has star-shaped gray hairs and thorns. The stem is dark purple with black light. Leaves are alternate phyllotaxis, 15 ~ 35cm long with petiole and pointed end. The flowers bloom in June-September, between the nodes of the stem and branch, the flower stalk comes out, and a lot of purple flowers of several flowers run. The calyx is purple. The shape of the fruit varies depending on the varieties, such as egg shape, ball shape, and long shape. In Korea, it mainly grows long branches with long shape.

There are many horticultural varieties, each of which is cytogenetically very close to make hybrids. A single hybrid is strong because it is powerful and not only disease-resistant, but also harvested and mated easily. The fruit is steamed and eaten as herbs or as whole grains, and it is eaten with steamed eggplant.

Meanwhile, in recent years, many cosmetic products using natural products have been developed to reduce skin irritation caused by various chemical substances and the like. In addition to low adverse effects on skin, natural materials have recently become increasingly appreciated as a cosmetic raw material as consumers' response to cosmetics using natural materials has increased.

The inventors of the present invention have studied the applicability of various natural products to cosmetics. As a result, it has been found that the extracts of ganoderma lucidum are fermented with mycelia of black yeast, and the skin moisturizing effect, skin soothing effect, antioxidant effect, The effect of improving the barrier was confirmed, and the present invention was completed.

Korean Patent No. 10-2015-0108065 relates to a cosmetic composition having antioxidant and antiinflammatory effects containing eggplant extracts, Korean Patent No. 10-1434219 discloses a supercritical extraction method of a polyphenol compound derived from a branch, It is stated that the antioxidant effect of the polyphenol compound derived from the branch extracted by the method is used as a cosmetic composition.

Therefore, the inventors of the present invention are based on the surprising result that the skin moisturizing effect is greatly improved when producing the eggplant seed extract using ultrasonic extraction instead of the conventional methods such as hot water extraction, room temperature extraction and warming extraction. Accordingly, it is an object of the present invention to provide a cosmetic composition comprising an extract of eggplant sonicated extract as an active ingredient. The present invention relates to a natural extract obtained by fermenting a ganoderma lucidum extract with a mycelia of black yeast and an effective ingredient thereof as a skin moisturizing effect, a skin soothing effect, an antioxidant The effect of improving the skin barrier, and finally, the present invention has been completed.

It is an object of the present invention to provide a cosmetic composition containing a black yeast fermented bovine sonic extract, specifically a black yeast fermented bovine sonal extract obtained by fermenting a bovine sonic extract with black fungus.

Preferably, the cosmetic composition may be for skin moisturizing effect, skin soothing action, antioxidative effect, skin barrier improvement.

Preferably, the black yeast fermented egg sonic extract is obtained by fermenting the egg sonicated extract for 1 to 7 days while maintaining the pH at 5 to 7 under a temperature condition of 20 to 40 ° C as a black yeast extract.

In addition, the cosmetic composition may contain 0.0001 to 30.0% by weight of black yeast fermented gruel extract based on the total weight of the cosmetic composition.

Preferably the cosmetic composition is in the form of a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray And can have any one of the formulations selected.

The present invention can provide a cosmetic composition containing the black yeast fermented egg sonic extract, which is applicable to skin cosmetics and is harmless to the human body and is highly safe, as an active ingredient. It is possible to provide a cosmetic composition having a skin moisturizing effect, a skin soothing effect, an antioxidative effect and a skin barrier improving effect as a black yeast fermented bovine yeast extract obtained through ultrasonic extraction and black yeast fermentation.

The cosmetic composition containing the black yeast fermented ginseng sonic extract of the present invention is superior in skin moisturizing effect to the existing cosmetic composition containing the extract of bark extract.

Unless defined otherwise, all technical terms used in the present invention have the following definitions and are consistent with the meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. Also, preferred methods or samples are described in this specification, but similar or equivalent ones are also included in the scope of the present invention.

The term "about" is used herein to refer to a reference quantity, a level, a value, a number, a frequency, a percentage, a dimension, a size, a quantity, a weight or a length of 30, 25, 20, 25, 10, 9, 8, 7, Level, value, number, frequency, percentage, dimension, size, quantity, weight or length of a variable, such as 4, 3, 2 or 1%.

Throughout this specification, the words "comprises" and "comprising ", unless the context requires otherwise, include the steps or components, or groups of steps or elements, Steps, or groups of elements are not excluded.

The inventors of the present invention have investigated the applicability of cosmetic preparations containing nutrient supernatant extracts from various natural products to various cosmetics, and found that antioxidative, moisturizing and skin barrier improvement effects can be expected as a cosmetic product. An extract having such excellent efficacy was obtained in a maximally high yield to develop a cosmetic composition showing more excellent effects.

The cosmetic composition of the present invention is a cosmetic composition containing black yeast fermented egg sonic extract obtained through ultrasound extraction of gut seeds and fermentation of black yeast.

The cosmetic composition of the present invention containing black yeast fermented eggplant sonicated extract is suitable as a cosmetic composition for skin moisturizing effect, skin soothing action, antioxidant effect and skin barrier improving effect.

In order to obtain the highest yield of the seeds having various potencies, the present inventors treated the germ with ultra high pressure extraction method and found that the content of the active ingredient was increased through black yeast fermentation, and applied to cosmetics to antioxidation, It was confirmed that there is an effect of improving the skin barrier, thereby completing the present invention.

The extract of the root extract which is an active ingredient of the cosmetic composition of the present invention can be produced by using a conventional solvent known in the art, that is, under the conditions of ordinary temperature and pressure, using a conventional solvent. That is, the extract of Ganoderma lucidum, which is an active ingredient of the composition of the present invention, can be dissolved in water, an anhydrous or a lower alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate and 1,3-butylene glycol, propylene glycol, dichloromethane, In an extraction solvent selected from the group consisting of chloroform, ethyl ether, butylene glycol, hexane, and mixtures thereof, followed by extraction with an ultrasonic extractor for about 1 to 12 hours. The time of the ultrasonic extraction may be varied depending on the amount of the sample to be extracted, and the result of the extraction may be filtered or purified.

Among the above solvents, alcohols are preferable, and ethanol is more preferable. At this time, the ethanol is preferably 70% ethanol. The suitable amount of the extraction solvent is 1 to 15 times, more preferably 5 to 10 times, and most preferably 7 times the dry weight of the seeds.

In addition, the eggplant seed extract of the present invention may be prepared into a powder state by an additional process such as vacuum distillation and freeze-drying or spray drying.

In addition, the branched sonic extract of the present invention means that the produced eggplant seeds are extracted with an ultrasonic extractor for about 1 to 12 hours while maintaining a pH of 5 to 7 at a temperature condition (room temperature) of 20 to 40 캜.

In addition, the eggplant seed extract of the present invention may be prepared into a powder state by an additional process such as vacuum distillation and freeze-drying or spray drying.

The black yeast fermented eggplant seed extract can be prepared by fermenting the above seed extracts with black yeast. Specifically, the yeast extract can be prepared by the following method. First, 1 to 5% by weight of glucose is added as a carbon source to the eggplant seed extract, and 0.1 to 1% by weight, preferably 0.2 to 0.5% by weight of peptone is further added to sterilize. It can be cultured by inoculating a culture medium of Aureobasidium pullulans strain so as to have a concentration of 25 g / L. The culture can be carried out using a 5 L fermenter for 1 to 7 days at 20 to 40 ° C while maintaining the pH at 5-7. After culturing, the culture broth was centrifuged and the culture was firstly removed and sterilized (121 ° C, 15 minutes, 1.5 atm) to prevent further culture.

After the fermentation, the fermented broth obtained by first removing the cultured bacteria is added with ethanol to make a final 70% (v / v) ethanol aqueous solution, refluxed, cooled, and filtered. When the filtration is completed, the extract obtained in the extracting step is transferred to a concentration tank and concentrated under reduced pressure or freeze-dried at 50 ° C or lower to prepare the black yeast eggplant fermentation extract of the present invention.

According to a preferred embodiment of the present invention, the black yeast fermented eggplant seed extract to be added to the cosmetic composition may be contained in an amount of 0.0001 to 30.0% by weight, preferably 0.01 to 20.0% by weight based on the total weight of the cosmetic composition, And most preferably 0.1 to 10.0% by weight. When the content of the fermented extract of black seed yeast is less than 0.0001% by weight, the effect of improving antioxidation, moisturizing and skin barrier is insignificant.

The ingredients contained in the cosmetic composition of the present invention include, as an active ingredient, the ingredients commonly used in cosmetic compositions in addition to the above-mentioned effective ingredients, such as conventional adjuvants such as stabilizers, solubilizers, vitamins, pigments and fragrances, .

The cosmetic composition of the present invention can be prepared into any of the formulations conventionally produced in the art and can be used in the form of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, , Oil, powder foundation, emulsion foundation, wax foundation and spray, but is not limited thereto.

More specifically, it can be manufactured in the form of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder.

When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

In the case where the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component is selected from aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters.

When the cosmetic composition of the present invention is a soap, a surfactant-containing cleansing agent, or a surfactant-free cleansing agent, it may be applied to the skin and then wiped off or removed or washed with water. As a specific example, the soap is liquid soap, powdered soap, solid soap and oil soap, and the surfactant-containing cleansing formulation is a cleansing foam, a cleansing water, a cleansing towel and a cleansing pack, , Cleansing lotion, cleansing water and cleansing gel, but is not limited thereto.

Hereinafter, the present invention will be described in more detail with reference to Production Examples. These production examples are only for explaining the present invention in more detail and that the scope of the present invention is not limited by these production examples according to the gist of the present invention, It will be obvious.

< Comparative Manufacturing Example  1> Manufacture of eggplant seed extract

The eggplant seeds were washed with purified water, dried, and then pulverized and finely ground using a sieve of 100 mesh. 70% ethanol was added thereto so as to have a weight of 100 g / L, and the mixture was refluxed three times for 5 hours. After cooling, the mixture was filtered through a filter of whatman # 3, concentrated under reduced pressure at 50 캜 or below and freeze- . The lyophilized product was dissolved in 50% glycerin to a concentration of 2% (W / V) to prepare a crude extract (Preparation Example 1).

< Comparative Manufacturing Example  2> Manufacture of eggplant extract

The eggplant seeds were selected as a sample, washed thoroughly, and finely pulverized so as to pass through a 300-mesh sieve. 70% ethanol was added thereto so as to have a weight of 100 g / L, and the mixture was refluxed at 95 ° C for 4 hours. After cooling, the mixture was filtered through Whatman # 3 filter paper and concentrated under reduced pressure at 50 ° C or below. And dried. The lyophilized product was dissolved in 2% (w / v) of 50% glycerin to prepare an extract of guar gum (Comparative Preparation Example 2).

< Comparative Manufacturing Example  3> Manufacture of eggplant sonicate extract

Egg seeds were prepared by using an ultrasonic extractor. Egg seeds were selected as a sample, washed thoroughly, and finely pulverized so as to pass through a 300-mesh sieve. 70% ethanol was added thereto so as to have a weight of 100 g / L, and the mixture was extracted with an ultrasonic wave extractor (natural solution) at room temperature for 4 hours. After cooling, the mixture was filtered through a Whatman # 3 filter paper, Concentrated under reduced pressure and lyophilized. The lyophilized product was dissolved in 50% glycerin to a concentration of 2% (W / V) to prepare edible sonic extract (Comparative Preparation Example 3).

< Comparative Manufacturing Example  4> Preparation of supercritical supercritical extract

The seeds were washed with purified water, dried and crushed into small pieces, and 1 g of the sample was placed in a supercriticalfluid extractor (Jasco, Japan) and a liquid chromatography pump (Supercritical fluid chromatograph, Jasco, Japan) The pure carbon dioxide was supercritical at 60 ℃ and 300 atmospheres pressure, and the extracts were given to the glass tube with a volume of 10 ㎖ for 40 minutes every 4 minutes while flowing butyl acetate in the co - solvent. For the supercritical extraction, 60 ° C and 300 atmospheres showed the best results. The extract was dried with a rotary evaporator to obtain a dry weight of 30.9 mg / g. The dried eggplant-extracted powder was dissolved in 50% 1,3-butylene glycol and used.

< Comparative Manufacturing Example  5> Preparation of Solvent Extracts

The branches were washed with purified water, dried and then ground to a fineness with a sieve of 100 mesh. 70% ethanol was added thereto so as to have a weight of 100 g / L, and the mixture was refluxed three times for 5 hours. After cooling, the mixture was filtered through Whatman # 3 filter paper and concentrated under reduced pressure and freeze-dried at 50 ° C or lower. The lyophilized product was dissolved in 50% glycerin to 2% (w / v) to prepare a solvent extract.

< Manufacturing example  1> Black yeast  Preparation of Fermented Eggplant Ultrasonic Extract

The branched sonic extract of the present invention may be prepared in powder form by an additional process such as vacuum distillation and freeze drying or spray drying.

The black yeast fermented eggplant sonicated extract can be prepared by fermenting the eggplant sonicated extract with black yeast. Specifically, the yeast extract can be prepared by the following method. First, 1 to 5% by weight of glucose is added as a carbon source to the eggplant sonicate extract, and 0.1 to 1%, preferably 0.2 to 0.5% by weight of peptone is further added to sterilize. It can be cultured by inoculating a culture medium of Aureobasidium pullulans strain so as to have a concentration of 25 g / L. The culture can be carried out using a 5 L fermenter for 1 to 7 days at 20 to 40 ° C while maintaining the pH at 5-7. After culturing, the culture broth was centrifuged and the culture was firstly removed and sterilized (121 ° C, 15 minutes, 1.5 atm) to prevent further culture.

After the fermentation, the fermented broth obtained by first removing the cultured bacteria is added with ethanol to make a final 70% (v / v) ethanol aqueous solution, refluxed, cooled, and filtered. When the filtration is completed, the extract obtained in the extraction step is transferred to a concentration tank and concentrated under reduced pressure or freeze-dried at 50 ° C or lower to prepare the black yeast fermented bovine endoskeletal extract of the present invention.

< Prescription example  1>

Example 1 of the cream containing black yeast fermented eggplant sonicate extract is shown in Table 1 below.

ingredient Content (unit:% by weight) Black yeast fermented eggplant sonicated extract (Preparation Example 1) 5.0 glycerin 5.0 Sodium hyaruronate 0.1 Polysorbate 60 1.5 Mineral oil 5.0 Squalane 3.0 Dimethicone 2.0 Triethanolamine 1.0 Chin type monostearin glycerin 2.0 Cetearyl alcohol 2.2 Stearic acid 1.5 Sorbitan stearate 0.6 Sodium magnesium silicate 0.1 Betaine 3.0 antiseptic a very small amount incense a very small amount Pigment a very small amount Purified water Balance system 100

< Comparative Prescription Example  1>

Comparative Example 1 of the cream in cosmetics containing eggplant sonicate extract is shown in Table 2 below.

ingredient Content (unit:% by weight) Extract of eggplant seeds (Comparative Preparation Example 3) 5.0 glycerin 5.0 Sodium hyaruronate 0.1 Polysorbate 60 1.5 Mineral oil 5.0 Squalane 3.0 Dimethicone 2.0 Triethanolamine 1.0 Chin type monostearin glycerin 2.0 Cetearyl alcohol 2.2 Stearic acid 1.5 Sorbitan stearate 0.6 Sodium magnesium silicate 0.1 Betaine 3.0 antiseptic a very small amount incense a very small amount Pigment a very small amount Purified water Balance system 100

< Comparative Prescription Example  2>

Comparative Example 2 of the cream containing no eggplant sonicate extract is shown in Table 3 below.

ingredient Content (unit:% by weight) glycerin 5.0 Sodium hyaruronate 0.1 Polysorbate 60 1.5 Mineral oil 5.0 Squalane 3.0 Dimethicone 2.0 Triethanolamine 1.0 Chin type monostearin glycerin 2.0 Cetearyl alcohol 2.2 Stearic acid 1.5 Sorbitan stearate 0.6 Sodium magnesium silicate 0.1 Betaine 3.0 antiseptic a very small amount incense a very small amount Pigment a very small amount Purified water Balance system 100

< Comparative Prescription Example  3>

Comparative formulation example 3 of cream in cosmetics containing eggplant seed extract is shown in Table 4 below.

ingredient Content (unit:% by weight) Eggplant seed extract (Comparative Preparation Example 1) 5.0 glycerin 5.0 Sodium hyaruronate 0.1 Polysorbate 60 1.5 Mineral oil 5.0 Squalane 3.0 Dimethicone 2.0 Triethanolamine 1.0 Chin type monostearin glycerin 2.0 Cetearyl alcohol 2.2 Stearic acid 1.5 Sorbitan stearate 0.6 Sodium magnesium silicate 0.1 Betaine 3.0 antiseptic a very small amount incense a very small amount Pigment a very small amount Purified water Balance system 100

< Comparative Prescription Example  4>

Comparative Example 4 of the cream containing eggplant warm-up extract is shown in Table 5 below.

ingredient Content (unit:% by weight) Egg warming extract (Comparative Preparation Example 2) 5.0 glycerin 5.0 Sodium hyaruronate 0.1 Polysorbate 60 1.5 Mineral oil 5.0 Squalane 3.0 Dimethicone 2.0 Triethanolamine 1.0 Chin type monostearin glycerin 2.0 Cetearyl alcohol 2.2 Stearic acid 1.5 Sorbitan stearate 0.6 Sodium magnesium silicate 0.1 Betaine 3.0 antiseptic a very small amount incense a very small amount Pigment a very small amount Purified water Balance system 100

< Comparative Prescription Example  5>

Example 5 of comparative formulation of a cream containing a supercritical extract of a guar gum is shown in Table 6 below.

ingredient Content (unit:% by weight) Supernatant supercritical extract (Comparative Preparation Example 5) 5.0 glycerin 5.0 Sodium hyaruronate 0.1 Polysorbate 60 1.5 Mineral oil 5.0 Squalane 3.0 Dimethicone 2.0 Triethanolamine 1.0 Chin type monostearin glycerin 2.0 Cetearyl alcohol 2.2 Stearic acid 1.5 Sorbitan stearate 0.6 Sodium magnesium silicate 0.1 Betaine 3.0 antiseptic a very small amount incense a very small amount Pigment a very small amount Purified water Balance system 100

< Comparative Prescription Example  6>

Comparative Example 6 of the cream containing the solvent extract is shown in Table 7 below.

ingredient Content (unit:% by weight) (Comparative Preparation Example 6) 5.0 glycerin 5.0 Sodium hyaruronate 0.1 Polysorbate 60 1.5 Mineral oil 5.0 Squalane 3.0 Dimethicone 2.0 Triethanolamine 1.0 Chin type monostearin glycerin 2.0 Cetearyl alcohol 2.2 Stearic acid 1.5 Sorbitan stearate 0.6 Sodium magnesium silicate 0.1 Betaine 3.0 antiseptic a very small amount incense a very small amount Pigment a very small amount Purified water Balance system 100

< Experimental Example  1> Antioxidant effect measurement

Antioxidant activity of the present invention was compared to that of Preparation Example 1 and Comparative Preparations 1 to 5 by the Tiobarbitric acid method (TBA assay) using a lipid peroxidation system by Fenton reaction. That is, in 5 ml of 25 mM Trizma hydrochloric acid / 0.75 mM potassium chloride buffer containing 10 에틸 of ethyl linoleate, 0.2% sodium dodecylsulfoxide (SDS), 10 탆 ol of ferrous chloride and 2 탆 ol of hydrogen peroxide, The dimethylsulfoxide (DMSO) test solution of the extract prepared in 1 to 5 was added, and the mixture was incubated at 55 ° C for 16 hours. Then, 50 μl of 4% dibutylhydroxyltoluene (BHT) was added to stop the reaction. 0.3 ml of this solution was put into a test tube, and 3.0 ml of 0.05 N hydrochloric acid and 0.67% thiobarbituric acid (TBA) solution were added and boiled for 30 minutes. After cooling the reaction solution, 4.0 ml of 85% butanol was added, and the absorbance of the butanol layer after centrifugation was measured at 535 nm. The antioxidative effect of each extract was calculated by the following equation (1).

The results are shown in Table 8 below.

Test substance Antioxidative effect(%) Experimental concentration (10 / / ml) Experimental concentration (50 / / ml) Experimental concentration (100 / / ml) Comparative Preparation Example 1 35 55 60 Comparative Production Example 2 10 15 15 Comparative Production Example 3 22 35 49 Comparative Production Example 4 25 35 49 Comparative Preparation Example 5 5 8 10 Production Example 1 55 68 90

It can be seen that the antioxidative effects of Preparation Example 1 and Comparative Preparation Examples 1 to 5 were mostly high, and the antioxidative effect of Preparation Example 1 according to the present invention was particularly excellent.

Preparation Example 1 and Comparative Preparations 1 to 5 and ascorbic acid, dl-alpha-tocopherol, beta-carotene and dibutylhydroxyltoluene (BHT), which are existing antioxidants, Were measured by the same method as Experimental Example 1, and the IC ₅₀ values of 50% inhibition concentration were compared. The results are shown in Table 9 below.

Test substance IC ₅₀ (10 μg / ml) Comparative Preparation Example 1 45.9 Comparative Production Example 2 15.8 Comparative Production Example 3 30.5 Comparative Production Example 4 31.8 Comparative Preparation Example 5 10.1 Production Example 1 50.5 Ascorbic acid 21.90 Dl-alpha-tocopherol &lt; / RTI &gt; 3.36 Beta-carotene 29.8 Dibutylhydroxyltoluene (BHT) <1

Production Example 1 and Comparative Production Examples 1 to 5 showed high activities comparable to those of other antioxidants, and in particular, Production Example 1 shows high activity. Especially, these extracts showed significant antioxidative activity despite the extract of natural products. The antioxidant activity was improved by fermentation of black yeast.

&Lt; Experimental Example 2 > Evaluation of skin moisturizing effect of black yeast fermented eggplant sonicated extract

Black yeast fermented egg sonic extract cream (Formulation Example 1) and Comparative Prescription Examples 1 to 6 The effect of improving the wrinkles of the cream was evaluated as follows.

Twenty-five to twenty-four patients without skin disease were divided into two groups of 20 patients per group. The cream of each of the prescription example 1 and comparative prescription examples 1 to 6 was administered twice daily Month and then applied to the face and forehead. The skin conductivity was measured by using a corneometer (CM820 courage Khazaka electronic GmbH, Germany) under the constant temperature and humidity conditions (24 ° C, 40% humidity) before the start of application, and the skin conductivity was measured at 1 week, 2 weeks, The rate of increase in skin conductivity (%) was measured and the rate of increase was evaluated. The results are shown in Table 10 below.

Experimental product After 1 week after 2 weeks After 3 weeks Prescription Example 1 55 60 69 Comparative Prescription Example 1 43 48 52 Comparative Prescription Example 2 17 20 24 Comparative Prescription Example 3 19 25 31 Comparative Prescription Example 4 25 28 35 Comparative Prescription Example 5 38 40 48 Comparative Prescription Example 6 15 20 22

As shown in Table 10, in the case of Cream Prescription Example 1 containing black yeast fermented bovine endosperm extract, the rate of skin conductivity increase was much higher than Comparative Prescription Examples 1 to 6. Since the skin conductivity is generally proportional to the skin moisture content, the above results confirm that the cosmetic composition containing the black yeast fermented egg sonic extract maintains the skin moisture content higher than that of the cosmetic composition not containing the black yeast fermented egg sonic extract. Skin irritation could not be observed in most of the subjects who applied this cosmetic composition to the skin.

&Lt; Experimental Example 3 > Evaluation of skin barrier improvement effect of black yeast fermented eggplant sonicated extract

In order to examine the skin barrier improvement effect of the cosmetic composition containing the black yeast fermented egg root sonicated extract of the present invention, the following experiment was conducted. In this experiment, TEWL (Trans-Epidemal Water Loss) was measured using a Tewameterr (TM300, Courage and Khazaka Electronic Co., Germany) for prescription examples 1 and comparative prescription examples 1 to 6 A comparative experiment was conducted and evaluated. Each sample was applied to the right and left faces of 40 women in their 20s and 40s. Percutaneous water loss before, 1 hour, 2 hours, 4 hours, and 6 hours after applying 1cm downward to the right side of 3cm from the eyes The results are shown in Table 1. The transdermal water loss was measured using a Tewameterr (TM300, Courage and Khazaka Electronic Co., Germany) three times and the average value was evaluated. The results are shown in Table 11 below.

division 1 hours 2 hours 4 hours 6 hours Prescription Example 1 9.2 8.5 7.8 6.8 Comparative Prescription Example 1 15.3 14.9 12.1 8.9 Comparative Prescription Example 2 22.0 21.5 20 16 Comparative Prescription Example 3 18.9 17.3 15.2 13.1 Comparative Prescription Example 4 17.0 16.2 14.2 12.1 Comparative Prescription Example 5 16.5 15.4 14.1 10.8 Comparative Prescription Example 6 15.3 16.5 14.5 10.9

As shown in Table 11, the amount of transdermal water loss was measured. As a result, the amount of transdermal water loss was reduced in the facial skin of an experimenter who applied cream containing black yeast fermented ginseng supernatant extract, Was found.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the same is by way of illustration and example only and is not to be construed as limiting the scope of the present invention. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

Claims (7)

A cosmetic composition comprising a black yeast fermented egg sonic extract obtained by fermenting an eggplant sonicate extract with black yeast. The method according to claim 1,
Wherein the cosmetic composition is for antioxidation. The method according to claim 1,
The cosmetic composition is for moisturizing the skin. The method according to claim 1,
The cosmetic composition is for improving skin barrier. The method according to claim 1,
The branched sonic extract may be prepared by mixing the seeds with purified water, anhydrous or lower alcohols having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate and 1,3-butylene glycol, propylene glycol, dichloromethane, chloroform, ethyl ether, Is added to an extraction solvent selected from the group consisting of glycerol, hexane, and mixtures thereof, and then extracted with an ultrasonic extractor for 1 to 12 hours. 6. The method according to any one of claims 1 to 5,
Wherein the black yeast fermented egg sonic extract is contained in an amount of 0.0001 to 30.0% by weight based on the total weight of the cosmetic composition. 6. The method according to any one of claims 1 to 5,
The cosmetic composition may be in the form of a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray &Lt; / RTI &gt;