KR101724529B1 - Cosmetic composition containing red sea cucumber extract fermented by aureobasidium pullulans - Google Patents

Abstract

The present invention relates to a cosmetic composition containing a fermentation broth of red sea ginseng black yeast obtained by fermenting red sea germ ( Stichopus Japonicus S.) using a mycelium of black yeast. Specifically, the present invention relates to a cosmetic composition comprising red sea ginseng obtained from a high yield of triterpenoid glycoside And a black yeast fermentation broth. INDUSTRIAL APPLICABILITY According to the present invention, it has excellent antioxidative effect, moisturizing effect and skin barrier improving effect, and has excellent stability without discoloration, separation and precipitation with respect to temperature change.

Description

TECHNICAL FIELD [0001] The present invention relates to a cosmetic composition comprising red sea germ extract of black sea germ, fermented with a black yeast mycelium,

The present invention relates to a cosmetic composition containing an extract of red sea ginseng as an active ingredient, and more particularly to a cosmetic composition containing a red sea germ extract of black sea ginseng fermented with mycelia of black yeast to enhance triterpenoid glycoside, ≪ / RTI >

The goal of cosmetics is to maintain beautiful and healthy skin, and also to delay the aging of naturally occurring skin as it gets older. In general, aging is caused by oxidative damage of biomaterials by free radicals. That is, reactive radicals such as active oxygen such as hydrogen peroxide, hydroxy radical, and superoxide radical peroxidize the lipid constituting the cell membrane to destroy the cell membrane, oxidize the protein, attack the nucleic acid and cause oxidative damage, Destroys and eventually kills cells. Skin characteristics that are most noticeable due to aging include wrinkles, decreased elasticity, and skin dullness. In addition to wrinkles and elasticity decline, after 40s, facial color is not clear and is often gray and dark. Especially for young women, when they experience childbirth, they have eosinophilia, blood circulation disorder, which causes their metabolic ability to fall and their skin to become rough and rough. When the metabolism ability is lowered and the skin becomes rough, the water content is also easily reduced. Generally, the moisture content of the skin is about 70% in the dermal layer, but decreases to the skin layer to about 10 to 30% in the keratin layer. The keratinocyte layer is known to have an important role in the moisturization of the skin due to the existence of a substance having a hydrophilic water retention capacity called a natural moisturizing factor (NMF). Normal keratinocyte layer maintains skin smoothness and softness and maintains normal body protection function when moisture is maintained at about 10 to 30%.

Therefore, proper maintenance of the moisture content of the keratinocyte layer is very important for preventing skin aging. However, other natural moisturizing factors such as sodium pyrrolidonecarboxylate (PCA-Na), sodium lactate, There is a problem that the emulsion stability of the cosmetic is impaired due to the strong electrolyte. Amino acids, collagen, elastin, etc. are also capable of moisturizing, but also had a limited ability to moisturize. The ability of the horny layer to retain moisture can be controlled by natural moisturizing factor (NMF), which consists of sebum components, amino acids, lactic acid, urea, and inorganic salts. And the development of a substance with high moisturizing effect is one of the important research subjects in cosmetics. Materials known to have antioxidant activity include ascorbic acid, dl-alpha-tocopherol, beta-carotene, dibutylhydroxyltoluene (BHT), and the like. Since the materials are used as a synthetic material, there is an urgent need to develop new antioxidative cosmetics which can be applied to skin safely and usefully, because they may cause problems of skin safety when used for a long time or in a large amount.

Therefore, studies on skin aging and moisturizing improvement are expected to be continuously carried out. Many cosmetic products using natural products are being developed in order to solve such skin problems and to reduce skin irritation caused by various chemicals. Natural materials are not only less harmful to the skin, but also have increased their value as a raw material for cosmetics due to the recent popularity of cosmetics using natural materials.

For this purpose, researches are being actively carried out to develop materials having excellent safety and skin beauty effect by searching skin physiological activity of various natural vegetable persons.

One of the natural materials, sea cucumber is a generic term for sea cucumbers belonging to the sea cucumber river of the dyke. It was named for its efficacy as ginseng. In the "Mizobo" and "Oozoku", there are "Hainan", "Munsun", and "Sukkyeon" Sea cucumbers "," sea cucumbers "," sea cucumbers "and" black cucumbers "are recorded in the" sea cucumbers ". In Japan, it is called nama-ko which means "sea turtle" because it is nocturnal and resembles a mouse. In Gyeongbuk Province, it is also called red ginseng.

The body of the sea cucumber is cylindrical, the abdomen is flat, the back is convex, but there is also no distinction between the back and the abdomen. The mouth at the tip of the body has 10 to 30 tapered tentacles. The tentacles absorb the nutrients by sucking the mangoes of the sea floor, and the rest are excreted outside the body. It is made of mucus and is slippery. There are a lot of calcareous small fragments in the skin, and it is used as a trait of the sea cucumber in the shape of a bone fragment. The body wall is thick with muscle and connective tissue. There are digestive tracts, cysts, blood vessels, nerves, reproductive organs in the body, crawling or swinging in the body and only a few species float in the deep sea. In the skin there are small fragments of calcareous bone fragments. There is a distinction between male and female, but it is difficult to distinguish by appearance. When the water temperature is 17 ℃ or less, the appetite is active and the exercise is active. When it is above 17 ℃, it stops eating, and when it is 25 ℃ or more, it sleeps in the summer. When you eat food, tentacles put sandy mud under the sea in your mouth, eat small creatures in sandy mud, eat sand and excrement out. When stimulated from the outside, the intestines are cut off and sent out of the anus, which is regenerated and regenerated. There are about 1,500 kinds of sea cucumbers in the world, and there are three kinds of sea cucumbers produced in Korea: red ginseng, red ginseng and black ginseng.

Sea cucumbers are the best ginseng preparations, and they were used as a tackifier in the oriental herbal medicine. It is because the sea cucumbers reduce the heat of the body while the blood is being blood-drawn (blood), and the kidney of Hako, who controls the excretory organs, is benefited to strengthen the tincture. Animals that sleep in the hibernation or the lower part of a horse for a certain period say it is good for the energetic. Also, for women, it is often a good food to complement the body during pregnancy, when a weak woman or placenta wears a sea cucumber instead of a ginseng to a pregnant woman who is weak.

The main active ingredients of red sea ginseng are chondroitin sulfate, terpenoide, glycoprotein, taurine and calcium.

Especially, triterpenoid glycoside derivatives have various effects on the skin. Asiaticoside, a pentacyclic triterpenoid glycoside, has antibacterial and antifungal properties, and has wound, gastric ulcer, It has been investigated that various skin diseases, mental diseases, tuberculosis, vein diseases and dementia have therapeutic effect. Recently, it has been reported that asiaticoside-derived derivatives protect cells from β-amyloid-induced cell death. Thus, triterpenoid glycoside derivatives are used in various applications such as edible, skin treatment, wound treatment, memory enhancer and tonic, and they are also used in the fields of medicine, cosmetics, functional foods, etc. (Immuno-Regulatory Effect on Centella asiatica L. Urban Extraction Solvent Associated with Ultrasonification Process. Korean J. Medicinal Crop Sci . 16 (5): 294 300 (2008).

In addition, many research reports have been made on triterpenoid saponins and ginsenosides. Among them, ginsenoside Rb1 is effective against gastritis and gastric ulcer and has antioxidant activity. Ginsenoside Rb2 has an antagonistic action against cancer toxin hormone and inhibits tumor angiogenesis. Ginsenoside Rc is a metabolite of intestinal bacteria The possibility of developing an anticancer drug has been reported. Ginsenoside Rd has been reported to have a therapeutic effect of nephrotoxicity and to protect the nervous system (ginsenoside content analysis of ginseng, goat ginseng and wild ginseng, and observation of changes after red ginseng). March).

However, triterpenoid glycosides are contained in trace amounts in sea cucumbers, which is not economical when used industrially.

Therefore, the present inventors have solved the problem by increasing the content of triterpenoid glycoside by fermenting the extract of red sea ginseng with black yeast mycelium. As a result of studying the applicability of various natural products to cosmetics, Terpenoid glycoside could confirm the various physiological activity effects such as moisturizing and skin elasticity on the skin and completed the present invention.

In order to achieve the above object, the present invention provides a cosmetic composition comprising a red sea ginseng extract, which is a natural extract, as fermented black yeast mycelia, and a red yeast black yeast fermentation liquid obtained as an active ingredient.

On the other hand, in the cosmetic composition of the present invention, the red sea cucumber extract may contain water, an anhydrous or a lower alcohol having 1 to 4 carbon atoms, acetone, glycerin, ethyl acetate, butyl acetate and 1,3-butylene glycol, propylene glycol, dichloromethane , And at least one solvent selected from the group consisting of chloroform, ethyl ether, butylene glycol, hexane, and mixtures thereof.

In addition, in the cosmetic composition of the present invention, the fermentation broth of red sea ginseng black yeast may be added in an amount of 0.0001 to 30.0% by weight based on the total weight of the cosmetic composition.

In addition, the cosmetic composition is preferably characterized in that the cosmetic composition gives an antioxidative effect.

In addition, preferably, the cosmetic composition is provided with a moisturizing effect.

In addition, the cosmetic composition preferably provides the cosmetic composition with skin barrier improving effect.

Still another object of the present invention is achieved by a cosmetic method characterized by applying the cosmetic composition to human skin to give an antioxidative effect, moisturizing effect, and skin barrier improving effect.

The present invention can provide a cosmetic composition comprising a red sea germ extract, which is a natural extract that is applicable to skin cosmetics and is harmless to the human body and is highly safe, and fermented with mycelia of black yeast, as an active ingredient.

In addition, the present invention can provide a cosmetic composition having antioxidative, moisturizing, and skin barrier improving effects using the red sea ginseng fermentation broth obtained by fermenting a natural extract, Red Sea Ginseng Extract, with a black yeast mycelium as an active ingredient.

In addition, the present invention can provide a composition in which the content of triterpenoid glycoside is increased by fermenting the extract of Red Sea Ginseng with black yeast.

Unless defined otherwise, all technical terms used in the present invention have the following definitions and are consistent with the meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. Also, preferred methods or samples are described in this specification, but similar or equivalent ones are also included in the scope of the present invention.

The term "about" is used herein to refer to a reference quantity, a level, a value, a number, a frequency, a percent, a dimension, a size, a quantity, a weight, or a length of 30, 25, 20, 25, 10, 9, 8, 7, Level, value, number, frequency, percent, dimension, size, quantity, weight or length of a variable, such as 4, 3, 2 or 1%.

Throughout this specification, the words "comprises" and "comprising ", unless the context requires otherwise, include the steps or components, or groups of steps or elements, Steps, or groups of elements are not excluded.

The inventors of the present invention have investigated the applicability of cosmetic ingredients containing red sea ginseng extract among various natural products, and have found that antioxidation, moisturizing and skin barrier improvement effects can be measured through various experiments, and as a result, the efficacy as a cosmetic product can be expected. The composition of the triterpenoid glycoside having such excellent effects was obtained at the highest possible yield, and a cosmetic composition having more excellent effect was developed.

The present invention relates to a cosmetic composition containing an extract prepared by fermenting red sea ginseng extract with black yeast mycelium. More particularly, the present invention relates to a cosmetic composition containing fermented red ginseng as a black yeast mycelium to produce red ginseng black yeast having an increased content of triterpenoid glycoside A cosmetic composition comprising a fermented extract as an active ingredient.

Aureobasidium pullulans is a microorganism used to make beer and wine, a fungus or mushroom herd, but is a generic name for single celled organisms that do not have mycelium, photosynthetic activity, or motility. It is a microorganism that humans have been using for food since 5000 years ago, and yeast itself is used as a cheap fat or protein source. It is rich in vitamin B group and contains vitamin D. In addition, black yeast is a microorganism that produces beta-glucan, and it is said that the black yeast fermented product alone has a moisturizing effect, antioxidant, and soothing effect. Black yeast beta-glucan contains a large amount of components known to have physiological activities such as anticancer and insulin secretion inhibition.

The cosmetic composition of the present invention as described above is a cosmetic composition containing a fermented extract of red sea ginseng black yeast ginseng obtained by fermenting red sea ginseng extract with black yeast mycelium.

The cosmetic composition of the present invention comprising the above components is suitable as a cosmetic composition for antioxidation, moisturizing and skin barrier improvement of skin.

The present invention fermented red sea gins into black yeast mycelium in order to obtain the triterpenoid glycoside ingredient having various effects with the highest possible yield. By this treatment, it was confirmed that the content of the active ingredient was increased, and it was confirmed that antioxidation, moisturizing and skin barrier improvement effect was obtained by applying it to cosmetics, thereby completing the present invention.

The Red Sea Ginseng extract, which is an effective ingredient of the cosmetic composition of the present invention, can be produced by using conventional solvents known in the art, that is, under the conditions of ordinary temperature and pressure, using conventional solvents. That is, the extract of Red Sea Ginseng, which is an effective ingredient of the composition of the present invention, can be prepared by mixing water, anhydrous or lower alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate and 1,3-butylene glycol, propylene glycol, dichloromethane, , Ethyl ether, butylene glycol, hexane, and mixtures thereof, or extracting the red sea gins through the use of the solvent, and then mixing them.

Among the above solvents, alcohols are preferable, and ethanol is more preferable. At this time, the ethanol is preferably 70% ethanol. The suitable amount of the extraction solvent is 1 to 15 times, more preferably 5 to 10 times, and most preferably 7 times the dry weight of red sea ginseng.

On the other hand, the red sea ginseng extract of the present invention includes not only the extract obtained by the above-mentioned extraction method, but also the extract obtained through ordinary purification process. For example, by separation using an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatographies (made for separation by size, charge, hydrophobicity or affinity), and the like, Fraction is also considered to be included in the red sea ginseng extract of the present invention.

In addition, the extract of red sea ginseng of the present invention may be prepared in powder form by an additional process such as vacuum distillation, freeze drying, spray drying and the like.

The red ginseng black yeast fermentation broth of the present invention means that the red ginseng extract obtained is fermented with the above black yeast ( Aureobasidium pullulans ) for 1 to 7 days while maintaining the pH at 5 to 7 under a temperature condition of 20 to 40 ° C.

According to a preferred embodiment of the present invention, the content of the red ginseng black yeast fermentation broth added to the cosmetic composition is 0.0001 to 30.0% by weight, preferably 0.001 to 25.0% by weight, 10.0% by weight. When the content of the red ginseng black yeast fermentation broth is less than 0.0001% by weight, the effect of improving antioxidation, moisturizing and skin barrier is not exhibited. When the content is more than 30% by weight, the effect of increasing the content is insignificant, There is a problem and it is not economical.

The ingredients contained in the cosmetic composition of the present invention include, as an active ingredient, the ingredients commonly used in cosmetic compositions in addition to the above-mentioned effective ingredients, such as conventional adjuvants such as stabilizers, solubilizers, vitamins, pigments and fragrances, .

The cosmetic composition of the present invention can be prepared into any of the formulations conventionally produced in the art and can be used in the form of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, , Oil, powder foundation, emulsion foundation, wax foundation and spray, but is not limited thereto.

More specifically, it can be manufactured in the form of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder.

When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

In the case where the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component is selected from aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters.

When the cosmetic composition of the present invention is a soap, a surfactant-containing cleansing agent, or a surfactant-free cleansing agent, it may be applied to the skin and then wiped off or removed or washed with water. The surfactant-containing cleansing formulation is a cleansing foam, a cleansing water, a cleansing towel, and a cleansing pack. The surfactant-free cleansing formulation may be a cleansing cream, , Cleansing lotion, cleansing water and cleansing gel, but is not limited thereto.

Hereinafter, the present invention will be described in more detail with reference to Production Examples. These production examples are only for explaining the present invention in more detail and that the scope of the present invention is not limited by these production examples according to the gist of the present invention, It will be obvious.

< Manufacturing example  1> Red sea ginseng  Extract preparation

Red Sea ginseng was washed with purified water, dried and then pulverized and finely ground using a sieve of 100 mesh. 70% ethanol was added to 100 g / L of Red Sea Ginseng, and the mixture was refluxed three times for 5 hours. After cooling, the mixture was filtered through Whatman # 3 filter paper, concentrated under reduced pressure at 50 ° C and freeze-dried. The lyophilized product was dissolved in 50% glycerin to 2% (w / v) to prepare a red sea ginseng extract (Preparation Example 1).

< Manufacturing example  2> Red sea ginseng  Fermented extract preparation

Red sea ginseng, which was fermented by fermentation of red sea ginseng, was selected as a sample, washed thoroughly, and finely pulverized so as to pass through a 300 mesh sieve. The red sea cucumber was added to the cultivation medium of Saccharomyces sp. (Saccharomyces cerevisiae KCTC 7904) at a concentration of 10 g / L. 2% (W / V) of glucose was added as a carbon source and 0.5% (w / v) of peptone was further added thereto. The cultures were incubated for 5 days at 37 ° C and pH 6 using a 5 L fermenter. After culturing, the culture was centrifuged to remove the cultures first.

Ethanol was added to the fermented red sea germ in which the culture was firstly removed so as to be a final 70% (v / v) ethanol aqueous solution, and the mixture was refluxed three times for 5 hours, cooled, and filtered through a filter of whatman # 2 . After the filtration was completed, the extract was transferred to a thickener, and the filtered extract was concentrated under reduced pressure and lyophilized. This powder was dissolved in 50% glycerin to 2% (W / V) to prepare a fermented extract of Red Sea Ginseng (Preparation Example 2).

< Manufacturing example  3> Red sea ginseng Black yeast  Preparation of fermentation broth

2 g of glucose was added as a carbon source to 5 g of the reduced-gassed red ginseng concentrate prepared in Preparation Example 1, and 0.5% of peptone was further added to sterilize. Here, 25 g / L of black yeast ( Aureobasidium pullulans ) were inoculated and cultured. The cultures were incubated for 7 days at 37 ° C and pH 6 using a 5 L fermenter. After culturing, the culture broth was centrifuged and the culture was firstly removed and sterilized (121 ° C, 15 minutes, 1.5 atm) to prevent further culture.

After the fermentation, the fermented red sea gins first removed from the culture was added with ethanol so as to become a final 70% (v / v) ethanol aqueous solution, refluxed three times for 5 hours, cooled, filtered with whatman # 3 filter paper Respectively. When the filtration is completed, the extract obtained in the extraction step is transferred to a concentration tank and concentrated under reduced pressure or freeze-dried at 50 ° C or lower. The lyophilized product was dissolved in 50% glycerin to a concentration of 2% (w / v) to prepare a red ginseng black yeast fermentation broth (Preparation Example 3).

< Prescription example  1>

Examples of formulations of creams in cosmetics containing red ginseng black yeast fermentation broth are shown in Table 1 below.

ingredient Content (unit:% by weight) Red sea ginseng black yeast fermentation broth 5.0 glycerin 5.0 Sodium hyaruronate 4.0 Polysorbate 60 1.5 Mineral oil 5.0 Squalane 3.0 Dimethicone 2.0 Triethanolamine 1.0 Chin type monostearin glycerin 2.0 Cetearyl alcohol 2.2 Stearic acid 1.5 Sorbitan stearate 0.6 Sodium magnesium silicate 0.1 Betaine 3.0 antiseptic a very small amount incense a very small amount Pigment a very small amount Purified water Balance system 100

<Comparison Prescription example  1>

       Comparative Example 1 of the cream containing no red ginseng black yeast fermentation broth is shown in Table 2 below.

ingredient Content (unit:% by weight) glycerin 5.0 Sodium hyaruronate 4.0 Polysorbate 60 1.5 Mineral oil 5.0 Squalane 3.0 Dimethicone 2.0 Triethanolamine 1.0 Chin type monostearin glycerin 2.0 Cetearyl alcohol 2.2 Stearic acid 1.5 Sorbitan stearate 0.6 Sodium magnesium silicate 0.1 Betaine 3.0 antiseptic a very small amount incense a very small amount Pigment a very small amount Purified water Balance system 100

<Comparison Prescription example  2>

Comparative Example 2 of the cream containing no red ginseng black yeast fermentation broth is shown in Table 3 below.

ingredient Content (unit:% by weight) Butylene glycol 5.0 Sodium hyaruronate 4.0 Polysorbate 60 1.5 Mineral oil 5.0 Squalane 3.0 Dimethicone 2.0 Triethanolamine 1.0 Chin type monostearin glycerin 2.0 Cetearyl alcohol 2.2 Stearic acid 1.5 Sorbitan stearate 0.6 Sodium magnesium silicate 0.1 Betaine 3.0 antiseptic a very small amount incense a very small amount Pigment a very small amount Purified water Balance system 100

<Comparison Prescription example  3>

Comparative Example 3 of the cream containing no red ginseng black yeast fermentation broth is shown in Table 4 below.

ingredient Content (unit:% by weight) Dipropylene glycol 5.0 Sodium hyaruronate 4.0 Polysorbate 60 1.5 Mineral oil 5.0 Squalane 3.0 Dimethicone 2.0 Triethanolamine 1.0 Chin type monostearin glycerin 2.0 Cetearyl alcohol 2.2 Stearic acid 1.5 Sorbitan stearate 0.6 Sodium magnesium silicate 0.1 Betaine 3.0 antiseptic a very small amount incense a very small amount Pigment a very small amount Purified water Balance system 100

< Experimental Example  1> Red Sea Ginseng  Active ingredient Triterpenoid  Substance material) Content measurement

The contents of the respective triterpenoid-based substances of Red Sea Ginseng of Production Examples 1 to 3 were analyzed by HPLC as shown in Table 5. [

time water Acetonitrile 0 80 20 5 80 20 25 72 28 35 67 33 40 59 41 45 20 80 47 20 80 50 80 20

The analytical column was analyzed with OptiPack C18, column temperature 30 ° and UV absorbance wavelength 203 nm, and the results are shown in Table 6 below.

Production Example 1 Production Example 2 Production Example 3 content Triterpenoid glycoside 3.02 3.25 4.85 %

As a result of measurement of the active ingredient content of Red Sea Ginseng in Production Examples 1 to 3, it was confirmed that the triterpenoid glycoside content of Production Example 3 was significantly higher than that of Production Examples 1 and 2.

From the above results, it was found that the triterpenoid glycoside component of the fermented broth of Red Sea Ginseng Black Yeast (Preparation Example 3), which was obtained by fermenting the red sea germ of the present invention using the black yeast mycelium, was compared with other extraction conditions (Production Examples 1 and 2) It can be confirmed that it increased greatly.

< Experimental Example  2> Antioxidant effect measurement

The antioxidant activity of the extracts prepared in Preparation Examples 1 to 3 of the present invention was measured by the Tiobarbituric acid method (TBA assay) using a lipid peroxidation system by Fenton reaction. That is, in 5 ml of 25 mM Trizma hydrochloric acid / 0.75 mM potassium chloride buffer containing 10 μl of ethyl linoleate, 0.2% sodium dodecylsulfoxide (SDS), 10 μmol of ferrous chloride and 2 μmol of hydrogen peroxide, The dimethylsulfoxide (DMSO) test solution of one extract was added and incubated at 55 ° C for 16 hours. Then, 50 μl of 4% dibutylhydroxyltoluene (BHT) was added to stop the reaction. 0.3 ml of this solution was put into a test tube, and 3.0 ml of 0.05 N hydrochloric acid and 0.67% thiobarbituric acid (TBA) solution were added and boiled for 30 minutes. After cooling the reaction solution, 4.0 ml of 85% butanol was added, and the absorbance of the butanol layer after centrifugation was measured at 535 nm. The antioxidative effect of each extract was calculated by the following equation (1).

The results are shown in Table 7 below.

Test substance Antioxidative effect(%) Experimental concentration (10 / / ml) Experimental concentration (50 / / ml) Experimental concentration (100 / / ml) Production Example 1 21 48 60 Production Example 2 27 50 62 Production Example 3 30 54 74

The antioxidative effects of the Red Sea Ginseng extracts of Preparation Examples 1 to 3 were mostly high, and the antioxidative effect of Preparation Example 3 according to the present invention was remarkably excellent.

Antioxidant activity of red sea ginseng extracts of Production Examples 1 to 3 and ascorbic acid, dl-a-tocopherol, beta-carotene and dibutylhydroxytoluene (BHT), which are antioxidants, Activity was measured by the same method as Experimental Example 2 to compare the IC ₅₀ of 50% inhibitory concentration.

The results are shown in Table 8 below

Test substance IC ₅₀ (10 μg / ml) Production Example 1 50.5 Production Example 2 48.4 Production Example 3 43.2 Ascorbic acid 21.90 Dl-alpha-tocopherol &lt; / RTI &gt; 3.36 Beta-carotene 29.8 Dibutylhydroxyltoluene (BHT) <1

The Red Sea Ginseng extracts of Preparation Examples 1 to 3 showed high activities comparable to those of other antioxidants, and in particular, Production Example 3 shows high activity. Especially, these red sea ginseng extracts have remarkable antioxidative activity despite being a mixture of natural products.

< Experimental Example  3> Red sea ginseng Black yeast  Evaluation of skin moisturizing effect of fermentation broth

The wrinkle improving effect of the cream of Red Sea Ginseng Black Yeast Fermentation Cream (Formulation Example 1) and Comparative Formulation Examples 1 to 3 was evaluated as follows.

Twenty-five to twenty-four patients without skin disease were divided into two groups each consisting of 20 patients per group. The nutritional creams of the prescription examples 1 and comparative prescription examples 1 to 3 were administered twice daily Month and then applied to the face and forehead. The skin conductivity was measured by using a corneometer (CM820 courage Khazaka electronic GmbH, Germany) under the constant temperature and humidity conditions (24 ° C, 40% humidity) before the start of application, and the skin conductivity was measured at 1 week, 2 weeks, The rate of increase in skin conductivity (%) was measured and the rate of increase was evaluated. The results are shown in Table 9 below.

Experimental product After 1 week after 2 weeks After 3 weeks Prescription Example 1 43 48 52 Comparative Prescription Example 1 17 20 24 Comparative Prescription Example 2 26 30 35 Comparative Prescription Example 3 19 25 31

As shown in Table 9, in the case of the cream containing the fermentation broth of Red Sea Ginseng Black (Yeast Formulation 1), the rate of increase in skin conductivity was much higher than that of Comparative Formulation Examples 1 to 3. In general, the skin conductivity is proportional to the skin moisture content, so that the above results confirm that the cosmetic composition containing the red sea ginseng black yeast keeps the skin moisture content higher than that of the cosmetic composition not containing it. Skin irritation could not be observed in most of the subjects who applied this cosmetic composition to the skin.

< Experimental Example  4> Red sea ginseng Black yeast  Evaluation of skin barrier improvement effect of fermentation broth

In order to investigate the skin barrier improvement effect of cosmetics containing the red ginseng black yeast fermentation broth of the present invention, the following experiment was conducted. In this experiment, the cosmetic compositions of Formulation Example 1 and Comparative Preparations 1 and 2 were applied to a human using a Tewameterr (TM300, Courage and Khazaka Electronic Co., Germany) for trans-epidemic water loss (TEWL) A comparative experiment was conducted and evaluated. Each sample was applied to the right and left faces of 40 women in their 20s and 40s. Percutaneous water loss before, 1 hour, 2 hours, 4 hours, and 6 hours after applying 1cm downward to the right side of 3cm from the eyes The results are shown in Table 1. The transdermal water loss was measured using a Tewameter (TM300, Courage and Khazaka Electronic Co., Germany) three times and the mean value was evaluated. The results are shown in Table 10 below.

division 1 hours 2 hours 4 hours 6 hours Prescription Example 1 15.3 14.9 12.1 8.9 Comparative Prescription Example 1 17.9 16.7 13.6 11.4 Comparative Prescription Example 2 18.9 17.3 15.2 14.2

As shown in Table 10, the amount of transdermal water loss was measured. As a result, the amount of transdermal water loss was reduced in the facial skin of an experimenter who applied cream containing Red Sea Ginseng Black Yeast Fermentation Liquid, Could know.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the same is by way of illustration and example only and is not to be construed as limiting the scope of the present invention. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

Claims (8)

A cosmetic composition for antioxidation comprising a fermentation broth of red sea ginseng black yeast fermented with a black yeast mycelium extract of red sea ginseng,
Wherein the cosmetic composition is obtained by fermenting the black sea germ extract with the black yeast for 1 to 7 days while maintaining the pH at 5 to 7 under a temperature condition of 20 to 40 占 폚. delete delete delete delete The method according to claim 1,
The red sea ginseng extract is prepared by dissolving red sea gins in a solvent selected from the group consisting of purified water, methanol, ethanol, glycerin, ethyl acetate, butylene glycol, propylene glycol, dichloromethane, chloroform, ethyl ether, butylene glycol, hexane, &Lt; / RTI &gt; The method according to claim 1,
Wherein the red ginseng black yeast fermentation broth is contained in an amount of 0.0001 to 30.0% by weight based on the total weight of the cosmetic composition. The method according to claim 1,
The cosmetic composition may be in the form of a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray &Lt; / RTI &gt;