KR101951816B1 - Cosmetic composition containing vaccinium vitis-idaea fruit extract fermented by aureobasidium pullulans - Google Patents

Abstract

The present invention relates to a method for producing The present invention relates to a cosmetic composition containing fruit juice of vitis - idaea , which is fermented by using a black yeast mycelium, and more specifically to a cosmetic composition containing a fruit juice of black fruit juice obtained by obtaining a high yield of triterpenoid glycoside as an active ingredient. To a cosmetic composition containing the same. INDUSTRIAL APPLICABILITY According to the present invention, it has excellent antioxidative effect, moisturizing effect and skin barrier improving effect, and has excellent stability without discoloration, separation and precipitation with respect to temperature change.

Description

FIELD OF THE INVENTION The present invention relates to a cosmetic composition comprising a berry fruit juice obtained by fermenting a berry fruit extract with a black yeast mycelium,

The present invention relates to a cosmetic composition comprising a berry fruit juice extract obtained by fermenting berry fruit with black yeast mycelium as an active ingredient, and more particularly, to a cosmetic composition comprising a berry fruit extract obtained by fermenting berry fruit extract with black yeast mycelium to produce triterpenoid glycoside Which is an effective ingredient of a black fruit yeast fermentation broth.

The goal of cosmetics is to maintain beautiful and healthy skin, and also to delay the aging of naturally occurring skin as it gets older. In general, aging is caused by oxidative damage of biomaterials by free radicals. That is, reactive radicals such as active oxygen such as hydrogen peroxide, hydroxy radical, and superoxide radical peroxidize the lipid constituting the cell membrane to destroy the cell membrane, oxidize the protein, attack the nucleic acid and cause oxidative damage, Destroys and eventually kills cells. Skin characteristics that are most noticeable due to aging include wrinkles, decreased elasticity, and skin dullness. In addition to wrinkles and elasticity decline, after 40s, facial color is not clear and is often gray and dark. Especially for young women, when they experience childbirth, they have eosinophilia, blood circulation disorder, which causes their metabolic ability to fall and their skin to become rough and rough. When the metabolism ability is lowered and the skin becomes rough, the water content is also easily reduced. Generally, the moisture content of the skin is about 70% in the dermal layer, but decreases to the skin layer to about 10 to 30% in the keratin layer. The keratinocyte layer is known to have an important role in the moisturization of the skin due to the existence of a substance having a hydrophilic water retention capacity called a natural moisturizing factor (NMF). Normal keratinocyte layer maintains skin smoothness and softness and maintains normal body protection function when moisture is maintained at about 10 to 30%.

Therefore, proper maintenance of the moisture content of the keratinocyte layer is very important for preventing skin aging. However, other natural moisturizing factors such as sodium pyrrolidonecarboxylate (PCA-Na), sodium lactate, There is a problem that the emulsion stability of the cosmetic is impaired due to the strong electrolyte. Amino acids, collagen, elastin, etc. are also capable of moisturizing, but also had a limited ability to moisturize. The ability of the horny layer to retain moisture can be controlled by natural moisturizing factor (NMF), which consists of sebum components, amino acids, lactic acid, urea, and inorganic salts. And the development of a substance with high moisturizing effect is one of the important research subjects in cosmetics. In addition, substances known to have antioxidant activity include ascorbic acid, dl-alpha-tocopherol, beta-carotene, dibutylhydroxyltoluene (BHT) and the like Since these materials are used as synthetic products, they may cause problems of skin safety when they are used for a long period of time or in a large amount. Therefore, there is an urgent need to develop new antioxidative cosmetics that can be applied safely and effectively to skin.

Therefore, studies on skin aging and moisturizing improvement are expected to be continuously carried out. Many cosmetic products using natural products are being developed in order to solve such skin problems and to reduce skin irritation caused by various chemicals. Natural materials are not only less harmful to the skin, but also have increased their value as a raw material for cosmetics due to the recent popularity of cosmetics using natural materials.

For this purpose, researches are being actively carried out to develop materials having excellent safety and skin beauty effect by searching skin physiological activity of various natural vegetable persons.

Triterpenoid glycoside derivatives have a variety of effects on the skin. The pentacyclic triterpenoid glycoside, asiaticoside, has antibacterial and antifungal properties. It has wound, Skin disease, mental illness, tuberculosis, vein disease, dementia, and the like. Recently, it has been reported that asiaticoside-derived derivatives protect cells from β-amyloid-induced cell death. Thus, triterpenoid glycoside derivatives are used in various applications such as edible, skin treatment, wound treatment, memory enhancer and tonic, and they are also used in the fields of medicine, cosmetics, functional foods, etc. (Immuno-Regulatory Effect on Centella asiatica L. Urban Extraction Solvent Associated with Ultrasonification Process. Korean J. Medicinal Crop Sci . 16 (5): 294 300 (2008).

In addition, many research reports have been made on triterpenoid saponins and ginsenosides. Among them, ginsenoside Rb1 is effective against gastritis and gastric ulcer and has antioxidant activity. Ginsenoside Rb2 has an antagonistic action against cancer toxin hormone and inhibits tumor angiogenesis. Ginsenoside Rc is a metabolite of intestinal bacteria The possibility of developing an anticancer drug has been reported. The ginsenoside Rd has been reported to have a therapeutic effect of nephrotoxicity and to protect the nervous system (Ginsenoside ginsenoside ginsenoside content analysis and change after red ginseng observation) month).

However, triterpenoid glycosides are contained in trace amounts in sea cucumbers, which is not economical when used industrially.

In order to solve these various problems, recently, many cosmetics using natural products have been developed to reduce skin irritation caused by various chemical substances and the like. Natural materials are not only less harmful to the skin, but also have increased their value as a raw material for cosmetics due to the recent popularity of cosmetics using natural materials.

Japanese Patent Laid-Open No. 2010-531816 relates to a composition containing a manganese extract, and it is described that a manganese extract containing polyphenol is effective for whitening, wrinkling, moisturizing and the like. Korean Patent No. 10-1587447 discloses a cosmetic composition containing a mixture obtained by supercritical extraction of a mixture of bamboo, ivy, liquorice, lanolin, lily, pine needle, And the like.

Therefore, the present inventors have solved this problem by increasing the content of triterpenoid glycoside by fermenting manganese fruit extract with black yeast mycelium. As a result of studying the applicability of various natural products to cosmetics, Triterpenoid glycoside could confirm the various physiological activity effects such as moisturization and skin elasticity on the skin, thus completing the present invention.

In order to accomplish the above object, the present invention provides a cosmetic composition comprising as an active ingredient a fruit juice of black rot fungus obtained by fermenting a natural extract, branucus fruit extract, with a black yeast mycelium.

Preferably, the cosmetic composition is for antioxidation.

Preferably, the cosmetic composition is for skin moisturizing.

Preferably, the cosmetic composition is for skin barrier improvement.

Preferably, the cosmetic composition is obtained by fermenting the above-described berry fruit extract with the black yeast for 1 to 7 days while maintaining the pH at 5 to 7 under a temperature condition of 20 to 40 ° C.

Preferably, the branberry fruit extract is obtained by extracting branberry fruit from a group consisting of purified water, methanol, ethanol, glycerin, ethyl acetate, butylene glycol, propylene glycol, dichloromethane, chloroform, ethyl ether, butylene glycol, hexane, And extracted using one or more selected solvents.

Preferably, the bran fruit juice black yeast fermentation broth is contained in an amount of 0.0001 to 30.0% by weight based on the total weight of the cosmetic composition.

Preferably, the cosmetic composition is in the form of a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, And has any one of the formulations selected.

The present invention can provide a cosmetic composition containing as an active ingredient a fruit juice of black rot fungus fermented with a black yeast mycelium extract, which is a natural extract which is applicable to skin cosmetics and which is harmless to the human body and which is highly safe.

Also, the present invention provides a cosmetic composition having antioxidative, moisturizing, and skin barrier improving effects, using as an active ingredient, a fermented broth of black fruit yeast obtained by fermenting a natural extract, branruit fruit extract, with a black yeast mycelium.

In addition, the fruit of the present invention can be fermented with black yeast to provide a composition having increased triterpenoid glycoside content.

Unless defined otherwise, all technical terms used in the present invention have the following definitions and are consistent with the meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. Also, preferred methods or samples are described in this specification, but similar or equivalent ones are also included in the scope of the present invention.

The term " about " is used herein to refer to a reference quantity, a level, a value, a number, a frequency, a percent, a dimension, a size, a quantity, a weight, or a length of 30, 25, 20, 25, 10, 9, 8, 7, Level, value, number, frequency, percent, dimension, size, quantity, weight or length of a variable, such as 4, 3, 2 or 1%.

Throughout this specification, the words " comprises " and " comprising ", unless the context requires otherwise, include the steps or components, or groups of steps or elements, Steps, or groups of elements are not excluded.

The inventors of the present invention have investigated the applicability of various natural products as a cosmetic ingredient containing a berry fruit extract, and found that antioxidation, moisturizing and skin barrier improvement effects can be measured through various experiments, and as a result, the efficacy as a cosmetic product can be expected. The composition of the triterpenoid glycoside having such excellent effects was obtained at the highest possible yield, and a cosmetic composition having more excellent effect was developed.

The present invention relates to a cosmetic composition containing an extract prepared by fermenting a berry fruit extract with black yeast mycelium. More particularly, the present invention relates to a cosmetic composition comprising berry fruit as a black yeast mycelium to produce a berry fruit with increased content of triterpenoid glycoside The present invention provides a cosmetic composition comprising a fermented black yeast extract as an active ingredient.

Raspberry ( Vaccinium vitis-idaea ) is a small evergreen shrub of azalea, a genus of plants. The leaves and berries of the bilberry are all used as medicines, especially rheumatism or fruit juicy fruits. In the past, it was used as a substitute for antibiotics. Recently, it is known to be rich in vitamins, called lingonberry.

In the present invention, the branberry fruit extract is obtained by extracting from various organs or parts (eg, leaves, branches, flowers, roots, stems, fruits, etc.) of the branberry fruit tree, preferably an extract obtained from the seedling or fruit , And most preferably from fruit.

Aureobasidium pullulans is a microorganism used to make beer and wine, a fungus or mushroom herd, but is a generic name for single celled organisms that do not have mycelium, photosynthetic activity, or motility. It is a microorganism that humans have used for food since 5000 years ago, and yeast itself is used as a cheap fat or protein source. It is rich in vitamin B group and contains vitamin D. In addition, black yeast is a microorganism that produces beta-glucan, and it is said that the black yeast fermented product itself has a moisturizing effect, antioxidant and soothing effect. Black yeast beta-glucan contains a large amount of components known to have physiological activities such as anticancer and insulin secretion inhibition.

The cosmetic composition of the present invention as described above is a cosmetic composition containing a fermented extract of berry fruit of black fruit obtained by fermenting berry fruit extract with black yeast mycelium.

The cosmetic composition of the present invention comprising the above components is suitable as a cosmetic composition for antioxidation, moisturizing and skin barrier improvement of skin.

The present invention fermented berry fruit with black yeast mycelium in order to obtain the triterpenoid glycoside ingredient with various effects in the highest possible yield. By this treatment, it was confirmed that the content of the active ingredient was increased, and it was confirmed that antioxidation, moisturizing and skin barrier improvement effect was obtained by applying it to cosmetics, thereby completing the present invention.

The berry fruit extract, which is an effective ingredient of the cosmetic composition of the present invention, can be prepared using conventional solvents known in the art, that is, under the conditions of ordinary temperature and pressure, using conventional solvents. That is, the extract of berry fruit, which is an active ingredient of the composition of the present invention, is selected from water, anhydrous or lower alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate and 1,3-butylene glycol, propylene glycol, dichloromethane, A solvent selected from the group consisting of chloroform, ethyl ether, butylene glycol, hexane, and mixtures thereof, or extracting the manganese fruit using the solvent, and then mixing them.

Among the above solvents, alcohols are preferable, and ethanol is more preferable. At this time, the ethanol is preferably 70% ethanol. The suitable amount of the extraction solvent is 1 to 15 times, more preferably 5 to 10 times, and most preferably 7 times the dry weight of the berry fruit.

On the other hand, the bran extract of the present invention includes not only the extract obtained by the above-mentioned extraction method, but also the extract obtained through a usual purification process. For example, by separation using an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatographies (made for separation by size, charge, hydrophobicity or affinity), and the like, Fraction is also considered to be included in the fruit of the present invention.

In addition, the fruit of the present invention may be produced in powder form by an additional process such as vacuum distillation, freeze-drying, spray drying and the like.

In addition, the fruit of the present invention can be used as a fermentation broth of a black yeast ( Aureobasidium (R)) for 1 to 7 days while maintaining the pH at 5 to 7 under a temperature condition of 20 to 40 ° C. pullulans . < / RTI >

According to a preferred embodiment of the present invention, the content of the manganese fermentation broth added to the cosmetic composition is 0.0001 to 30.0% by weight, preferably 0.001 to 25.0% by weight, and most preferably 0.1 To 10.0% by weight. When the content of the black fruit yeast fermentation broth is less than 0.0001% by weight, the effect of improving antioxidation, moisturizing and skin barrier is not exhibited. When the content is more than 30% by weight, the effect of increasing the content is insignificant. There is a problem with stability and it is not economical.

The ingredients contained in the cosmetic composition of the present invention include, as an active ingredient, the ingredients commonly used in cosmetic compositions in addition to the above-mentioned effective ingredients, such as conventional adjuvants such as stabilizers, solubilizers, vitamins, pigments and fragrances, .

The cosmetic composition of the present invention can be prepared into any of the formulations conventionally produced in the art and can be used in the form of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, , Oil, powder foundation, emulsion foundation, wax foundation and spray, but is not limited thereto.

More specifically, it can be manufactured in the form of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder.

When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

In the case where the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component is selected from aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters.

When the cosmetic composition of the present invention is a soap, a surfactant-containing cleansing agent, or a surfactant-free cleansing agent, it may be applied to the skin and then wiped off or removed or washed with water. The surfactant-containing cleansing formulation is a cleansing foam, a cleansing water, a cleansing towel, and a cleansing pack. The surfactant-free cleansing formulation may be a cleansing cream, , Cleansing lotion, cleansing water and cleansing gel, but is not limited thereto.

Hereinafter, the present invention will be described in more detail with reference to Production Examples. These production examples are only for explaining the present invention in more detail and that the scope of the present invention is not limited by these production examples according to the gist of the present invention, It will be obvious.

< Manufacturing example  1> Manger  Production of fruit extract

The berry fruit was washed with purified water, dried and then pulverized and finely ground using a sieve of 100 mesh. 70% ethanol was added thereto so as to have a weight of 100 g / L, and the mixture was refluxed three times for 5 hours. After cooling, the mixture was filtered through a filter of whatman # 3, concentrated under reduced pressure at 50 ° C or lower and freeze- . The lyophilized product was dissolved in 50% glycerin to a concentration of 2% (W / V) to prepare a fruit juice extract (Preparation Example 1).

&Lt; Preparation Example 2 >

Berries were fermented by fermentation, and the berries were selected as a sample, thoroughly washed, and finely pulverized so as to pass through a 300-mesh sieve. This was added to the cultivation medium of Saccharomyces sp. (Saccharomyces cerevisiae KCTC 7904) to give 20 g / L of manganese fruit. 2% (W / V) of glucose was added as a carbon source and 0.5% (w / v) of peptone was further added thereto. The culture was incubated for 1 to 7 days, preferably 5 days, while maintaining the pH at 5-7, preferably at pH 6, at 37 ° C in a 5 L fermenter. After culturing, the culture was centrifuged to remove the cultures first.

The fermented fruit of which the culture was firstly removed was refluxed three times for 5 hours by adding ethanol so as to be a final 70% (v / v) ethanol aqueous solution, cooled with water, filtered with a filter of whatman # 2 Respectively. After the filtration was completed, the extract was transferred to a thickener, and the filtered extract was concentrated under reduced pressure and lyophilized. The powder was dissolved in 50% glycerin to a concentration of 2% (W / V) to prepare a fruit extract of Banbury fruit (Preparation Example 2).

&Lt; Preparation Example 3 > Preparation of blackberry yeast fermentation broth

2% (W / V) glucose was added as a carbon source to 5 g of the manganese fruit depressurized concentrate prepared in Preparation Example 1, and further sterilized by adding 0.5% (w / v) of peptone. Here, 20 g / L of black yeast ( Aureobasidium pullulans ) were inoculated and cultured. The cultures were incubated for 7 days at 37 ° C and pH 6 using a 5 L fermenter. After culturing, the culture broth was centrifuged and the culture was firstly removed and sterilized (121 ° C, 15 minutes, 1.5 atm) to prevent further culture.

After the fermentation, the fermented fruit of the manganese fruit which had been firstly removed from the culture was refluxed three times for 5 hours by adding ethanol to make a final 70% (v / v) ethanol aqueous solution. After cooling, the mixture was cooled with whatman # 3 filter paper Filtered. When the filtration is completed, the extract obtained in the extraction step is transferred to a concentration tank and concentrated under reduced pressure or freeze-dried at 50 ° C or lower. The lyophilized product was dissolved in 50% glycerin to 2% (w / v) to prepare a fruit juice of black fruit juice (Preparation Example 3).

&Lt; Formulation Example 1 >

Formulation examples of creams in cosmetics containing berry fruit black yeast fermentation broth are shown in Table 1 below.

ingredient Content (unit:% by weight) Berry fruit black yeast fermentation broth (Preparation Example 3) 5.0 glycerin 5.0 Sodium hyaruronate 4.0 Polysorbate 60 1.5 Mineral oil 5.0 Squalane 3.0 Dimethicone 2.0 Triethanolamine 1.0 Chin type monostearin glycerin 2.0 Cetearyl alcohol 2.2 Stearic acid 1.5 Sorbitan stearate 0.6 Sodium magnesium silicate 0.1 Betaine 3.0 antiseptic a very small amount incense a very small amount Pigment a very small amount Purified water Balance system 100

<Comparison Formulation Example  1>

Comparative Formulation Example 1 of the cream containing no berry fruit black yeast fermentation liquid is shown in Table 2 below.

ingredient Content (unit:% by weight) glycerin 5.0 Sodium hyaruronate 4.0 Polysorbate 60 1.5 Mineral oil 5.0 Squalane 3.0 Dimethicone 2.0 Triethanolamine 1.0 Chin type monostearin glycerin 2.0 Cetearyl alcohol 2.2 Stearic acid 1.5 Sorbitan stearate 0.6 Sodium magnesium silicate 0.1 Betaine 3.0 antiseptic a very small amount incense a very small amount Pigment a very small amount Purified water Balance system 100

<Comparison Formulation Example  2>

Comparative Formulation Example 2 of the cream containing no fruit extract of berry fruit (Preparation Example 2) is shown in Table 3 below.

ingredient Content (unit:% by weight) Production Example 2 5.0 Sodium hyaruronate 4.0 Polysorbate 60 1.5 Mineral oil 5.0 Squalane 3.0 Dimethicone 2.0 Triethanolamine 1.0 Chin type monostearin glycerin 2.0 Cetearyl alcohol 2.2 Stearic acid 1.5 Sorbitan stearate 0.6 Sodium magnesium silicate 0.1 Betaine 3.0 antiseptic a very small amount incense a very small amount Pigment a very small amount Purified water Balance system 100

<Comparison Formulation Example  3>

Comparative Formulation Example 3 of cream containing no branberry fruit extract (Preparation Example 1) is shown in Table 4 below.

ingredient Content (unit:% by weight) Production Example 1 5.0 Sodium hyaruronate 4.0 Polysorbate 60 1.5 Mineral oil 5.0 Squalane 3.0 Dimethicone 2.0 Triethanolamine 1.0 Chin type monostearin glycerin 2.0 Cetearyl alcohol 2.2 Stearic acid 1.5 Sorbitan stearate 0.6 Sodium magnesium silicate 0.1 Betaine 3.0 antiseptic a very small amount incense a very small amount Pigment a very small amount Purified water Balance system 100

< Experimental Example  1> Manger  Active ingredient of fruit ( Triterpenoid  Substance material) Content measurement

The contents of the respective triterpenoid-based substances of the berry fruit of Preparative Examples 1 to 3 were analyzed using HPLC as shown in Table 5.

time water Acetonitrile 0 80 20 5 80 20 25 72 28 35 67 33 40 59 41 45 20 80 47 20 80 50 80 20

The analytical column was analyzed with OptiPack C18, column temperature 30 ° and UV absorbance wavelength 203 nm, and the results are shown in Table 6 below.

Production Example 1 Production Example 2 Production Example 3 content Triterpenoid glycoside 3.02 3.25 4.85 %

As a result of measurement of the active ingredient content of the fruit of the present invention, the triterpenoid glycoside component of the fruit juice of black fruit juice (Preparation Example 3), which was obtained by fermenting the fruit of the present invention with the black yeast mycelium, And it was confirmed that it greatly increased as compared with other extraction conditions (Production Examples 1 and 2).

< Experimental Example  2> Antioxidant effect measurement

The antioxidant activity of the extracts prepared in Preparation Examples 1 to 3 of the present invention was measured by the Tiobarbituric acid method (TBA assay) using a lipid peroxidation system by Fenton reaction. That is, in 5 ml of 25 mM Trizma hydrochloric acid / 0.75 mM potassium chloride buffer containing 10 μl of ethyl linoleate, 0.2% sodium dodecylsulfoxide (SDS), 10 μmol of ferrous chloride and 2 μmol of hydrogen peroxide, The dimethylsulfoxide (DMSO) test solution of one extract was added and incubated at 55 ° C for 16 hours. Then, 50 μl of 4% dibutylhydroxyltoluene (BHT) was added to stop the reaction. 0.3 ml of this solution was put into a test tube, and 3.0 ml of 0.05 N hydrochloric acid and 0.67% thiobarbituric acid (TBA) solution were added and boiled for 30 minutes. After cooling the reaction solution, 4.0 ml of 85% butanol was added, and the absorbance of the butanol layer after centrifugation was measured at 535 nm. The antioxidative effect of each extract was calculated by the following equation (1). The results are shown in Table 7.

Test substance Antioxidative effect(%) Experimental concentration (10 / / ml) Experimental concentration (50 / / ml) Experimental concentration (100 / / ml) Production Example 1 21 48 60 Production Example 2 27 50 62 Production Example 3 42 64 84

The antioxidative effects of the fruit extracts of Preparation Examples 1 to 3 were mostly high, and the antioxidative effect of Preparation Example 3 according to the present invention was remarkably excellent.

The extracts of bran fruits of Preparation Examples 1 to 3 and ascorbic acid, dl-alpha-tocopherol, beta-carotene and dibutylhydroxytoluene (BHT), which are conventional antioxidants, ) Was measured by the same method as Experimental Example 2 to compare the IC ₅₀ of 50% inhibitory concentration. The results are shown in Table 8 below.

Test substance IC ₅₀ (10 μg / ml) Production Example 1 50.5 Production Example 2 48.4 Production Example 3 34.3 Ascorbic acid 21.9 Dl-a-tocopherol &lt; / RTI &gt; 3.36 Beta-carotene 29.8 Dibutylhydroxyltoluene (BHT) <1

The fruit extracts of bran fruits of Preparation Examples 1 to 3 showed high activities comparable to those of other antioxidants, and in particular, Production Example 3 shows high activity. Especially, these fruit extracts have remarkable antioxidative activity despite being a mixture of natural products.

< Experimental Example  3> Manger  Fruit Black yeast  Evaluation of skin moisturizing effect of fermentation broth

The wrinkle-improving effect of the berry fruit black yeast fermentation broth cream (Formulation Example 1) and Comparative Formulation Examples 1 to 3 was evaluated as follows. Twenty-five to twenty-four patients without skin disease were divided into two groups each consisting of 20 patients per group. The nutritional creams of Formulation Example 1 and Comparative Formulation Examples 1 to 3 were administered twice daily for 1 month, I had to apply it to Park. The skin conductivity was measured by using a corneometer (CM820 courage Khazaka electronic GmbH, Germany) under the constant temperature and humidity conditions (24 ° C, 40% humidity) before the start of application, and the skin conductivity was measured at 1 week, 2 weeks, The rate of increase in skin conductivity (%) was measured and the rate of increase was evaluated. The results are shown in Table 9 below.

Experimental product After 1 week after 2 weeks After 3 weeks Formulation Example 1 43 48 52 Comparative Formulation Example 1 17 20 24 Comparative Formulation Example 2 26 30 35 Comparative Formulation Example 3 19 25 31

As shown in Table 9, in the case of the cream (Formulation Example 1) containing the citrus fruit black yeast fermentation broth, the rate of skin conductivity increase was much higher than that of Comparative Formulations 1 to 3. In general, the skin conductivity is proportional to the skin moisture content, so the above results confirm that the cosmetic containing the berry fruit juice of black fruit juice maintains the skin moisture content higher than the other cosmetic ingredients. Skin irritation could not be observed in most of the subjects who applied this cosmetic composition to the skin.

&Lt; Experimental Example 4 > Evaluation of skin barrier improvement effect of black fruit yeast fermentation broth

In order to examine the skin barrier improvement effect of the cosmetic composition containing the fermentation broth of black fruit yeast of the present invention, the following experiment was conducted. In this experimental example, the cosmetic material of Formulation Example 1 was evaluated by performing a comparative experiment using a Tewameterr (TM300, Courage and Khazaka Electronic Co., Germany) for the trans-epidemic water loss (TEWL) Each sample was applied to the right and left faces of 40 women in their 20s and 40s. Percutaneous water loss before, 1, 2, 4, and 6 hours was applied before and 3 cm from the eye. The results are shown in Table 1. The transdermal water loss was measured using a Tewameter (TM300, Courage and Khazaka Electronic Co., Germany) three times and the mean value was evaluated. The results are shown in Table 10 below.

division 1 hours 2 hours 4 hours 6 hours Formulation Example 1 15.3 14.9 12.1 8.9 Comparative Formulation Example 1 17.9 16.7 13.6 11.4 Comparative Formulation Example 2 18.9 17.3 15.2 14.2

As shown in Table 10, the amount of transdermal water loss was measured, and as a result, the amount of transdermal water loss was reduced in the facial skin of an experimenter who applied cream containing fruit juice of black fruit juice black yeast, and thus, it is more effective for improving skin barrier And it was found.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the same is by way of illustration and example only and is not to be construed as limiting the scope of the present invention. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

Claims (8)

A cosmetic composition for skin barrier improvement, comprising a fermented black fruit yeast fruit obtained by fermenting bran fruit extract with black yeast mycelium. The method according to claim 1,
Wherein said cosmetic composition is obtained by fermenting said berry fruit extract with said black yeast for 1 to 7 days while maintaining pH 5 to 7 under a temperature condition of 20 to 40 캜. The method according to claim 1,
The above-mentioned fruit of the manganese fruit is characterized in that the manganese fruit is at least one selected from the group consisting of purified water, methanol, ethanol, glycerin, ethyl acetate, butylene glycol, propylene glycol, dichloromethane, chloroform, ethyl ether, butylene glycol, Wherein the composition is extracted using a solvent. The method according to claim 1,
Wherein the bran fruit juice black yeast fermentation broth is contained in an amount of 0.0001 to 30.0% by weight based on the total weight of the cosmetic composition. The method according to claim 1,
The cosmetic composition may be in the form of a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray Of the total amount of the composition. delete delete delete