KR20150100288A - Cosmetic composition comprising the extract of crude drug fermentation using the black yeast - Google Patents

Abstract

The present invention relates to a cosmetic composition including an extract of a crude drug fermented by black yeast and, more specifically, to a composition for proliferation of skin cells, and a composition of cosmetics for preventing skin aging and cosmetics for improving skin moisturizing including Panax ginseng, Schisandra chinensis (Turcz.) Baill., Polygonatum odoratum var. pluriflorum, Astragalus membranaceus, Glycyrrhiza uralensis, Paeonia lactiflora, and Rehmannia glutinosa as active ingredients. Also, the present invention relates to a cosmetic composition containing a black yeast-fermented crude drug extract obtained by fermenting crude drugs including Panax ginseng by black yeast and filtering the same. According to the present invention, the cosmetic composition is characterized by having excellent skin cell proliferation and skin aging prevention effects, and having excellent skin moisturizing effect.

Description

[0001] The present invention relates to a cosmetic composition containing an extract of crude herbals fermented with black yeast,

The present invention relates to a cosmetic composition comprising an herbal medicine extract fermented with black yeast. More particularly, the present invention relates to a skin cell proliferation composition, a composition for promoting collagen synthesis, and a cosmetic composition for enhancing skin moisturization, which comprises an extract of black yeast fermented extract of ginseng, omija, roundworm, hwanggi, licorice,

The cosmetic composition of the present invention exhibits skin cell proliferation, skin aging prevention effect and moisturizing effect without irritation to the skin, and thus is useful for prevention of complex skin aging phenomenon such as wrinkles, reduction of elasticity and decrease of moisturizing power.

The skin, which is the largest organ of the human body, occupies about 16% of the whole human body volume. The skin is in direct contact with the external environment, and has many important harmful factors to intrude into the human body, such as temperature, humidity and ultraviolet rays. It plays a role. However, with age, skin cells are damaged due to various pollutants, strong ultraviolet rays, stress and nutritional deficiency, cell proliferation is not properly performed, and wrinkles, loss of elasticity and keratinization occur in the skin.

Originally, normal healthy skin has built up a defense system to protect the skin from these external damage factors. However, the continuous external stimulus loses the protective effect of protecting the skin from external damage factors, and the ability to protect and proliferate Skin elasticity is reduced and wrinkles are generated.

Collagen and elastin present in the dermis in the structure of the skin are the major proteins that govern the elasticity of the skin. Biosynthesis of collagen fibers is affected by internal or external factors of the skin. Natural factors such as natural aging (decrease of estrogen) decrease cell activity and decrease collagen fiber. As external factors, active oxygen (free radicals) generated by overexposure of ultraviolet light and stress may react with the -SH group of the protein to inhibit enzyme activity, or enzymes such as matrix metalloproteinases (MMPS ) Collagenase, which causes wrinkling of the skin and aging of the elasticity reduction.

In order to inhibit or slow down the progress of skin aging, cosmetics or skin protectants containing various skin protecting ingredients such as retinol, retinoid, vitamin C, flavonoid and tocopherol are being developed. However, such a cosmetic or skin protecting agent has a weak potency in the final formulation and has a slight effect on skin irritation.

Currently, researches for the development of cosmetics capable of protecting skin cells from the external environment such as ultraviolet rays, activating cell proliferation ability, activating cell functions, and imparting a smooth moisturizing ability are actively under way. However, the conventional results are insufficient, and there is not much known about cosmetics which solve various kinds of aging phenomena such as wrinkles, elasticity reduction and moisturizing ability.

Accordingly, the present inventors have studied the applicability of ginseng and other herbal medicines as cosmetics among various natural products and measured their blood circulation improvement and elasticity effects through various experiments, and as a result, they found that the efficacy as a cosmetic product can be expected.

Ginseng (Panax ginseng) is a perennial plant belonging to the Araliaceae family. Its roots are called Ginseng Radix, and it is called Ginseng Radix. It is composed of ginseng (强壯), gangjeong (强血) ), Health (stomach), fatigue recovery, mental stability, is said to have the effect of sedation.

The main active ingredients of such ginseng include saponin, sapogenin, polyacetylene, pyrazine derivatives, and maltol. Ginseng saponin is divided into protopanaxadiol, protopanaxatriol and oleanonane saponins according to their chemical structure. Up to now, 19 kinds, 10 kinds, 1 The compounds of the species have been isolated and purified, and the diol and triol systems are known to have different pharmacological actions in the body. Ginseng saponin is known to have no toxicity, unlike other saponins, and does not exhibit hemolytic activity below 0.001%. It has been reported to have central nerve inhibitory action, protein synthesis promoting action, corticosteroid secretion promoting action, and immunity enhancing action .

Schisandra chinensis (Turcz.) Baill, Polygonatum odoratum var., Astragalus membranaceus ), licorice ( Glycyrrhiza glabra L.), peony ( Paeonia lactiflora , Rehmannia glutinosa Liboschtz var.) Is known to have the effects of exfoliation, antibacterial, anti-inflammatory action, wound healing, excellent moisture retention and blood circulation promotion.

Accordingly, the inventors of the present invention have attempted to find a method for maximizing the efficacy of herbal medicine extracts and to apply them to cosmetics. As a result, it has been found that the method of preparing the black yeast fermented herbal medicine extract of the present invention increases the efficacy on skin And applied to cosmetics to develop the composition for cell proliferation, the composition for preventing skin aging and the cosmetic composition having skin moisturizing effect of the present invention.

In order to achieve the above object, the present invention provides a cosmetic composition comprising an extract of Aureobasidium pullulans fermented herbal medicine, wherein the herbal medicine is a cosmetic composition which is ginseng, omija, roundworm, hwanggi, licorice, . The cosmetic composition may be used for skin cell proliferation, skin aging prevention, skin moisturizing promotion.

Preferably, the fermentation is carried out by fermenting ginseng, omija, roundweed, hwanggi, licorice, peony, and guinea fungus with Aureobasidium pullulans bacterium for 1 to 7 days while maintaining the pH of 5-7 under a temperature condition of 20-40 캜 It can be done.

Preferably, the extraction is carried out in a solvent selected from the group consisting of purified water, methanol, ethanol, glycerin, ethyl acetate, butylene glycol, propylene glycol, dichloromethane, chloroform, ethyl ether, butylene glycol, hexane, May be to extract using.

The black yeast fermented crude drug extract may be contained in an amount of 0.0001 to 30.0% by weight based on the total weight of the cosmetic composition.

According to the present invention, the black yeast fermented herbal medicine extract has an antioxidative effect superior to the usual herbal medicine extract (Experimental Example 1), cell activity (Experimental Example 2), cell migration effect (Experimental Example 3), skin moisturizing effect ) And skin elasticity improvement effect (Experimental Example 5).

Therefore, the cosmetic composition of the present invention comprising the black yeast fermented herbal medicine extract having various functions as an active ingredient can be usefully used because of its cell proliferation effect, collagen synthesis promoting effect, skin aging prevention effect and so on.

1 shows the antioxidative effect (free radical scavenging rate) of herbal medicine extract according to the present invention.
2 shows the cell proliferation rate of herbal medicine extract according to the present invention.
3 shows the cell migration effect of herbal medicine extracts according to the present invention.

The present invention relates to a cosmetic composition comprising as an active ingredient a fermented extract of a herbal medicine comprising ginseng, omija, roundworm, hwanggi, licorice, peony, and guinea as an active ingredient. More particularly, And extracting the obtained black yeast fermented herbal medicine as an active ingredient.

Aureobasidium pullulans has an elliptical shape resembling yeast cells, and when cultured, it is colored by the production of melanin pigment.

Black yeast produces pullulan, a kind of alpha-glucan, and is widely used in the fields of food additives and edible films. In addition, black yeast is a microorganism that produces water-soluble beta-glucan. The black yeast fermented product alone has a characteristic of having an effect such as moisturizing effect, antioxidant effect and soothing effect.

The extract of black yeast fermented herbal medicine contained in the cosmetic composition of the present invention is a black yeast fermented herbal medicine extract containing ginseng, omija, roundwort, hwanggi, licorice, peony and guinea fungus as a black yeast strain under the temperature condition of 20 to 40 캜, It can be obtained by fermentation for a day.

The herbs used in the present invention may be those prepared by a generally known method. These were thoroughly washed and pulverized, and finely granulated using 300 mesh. The herbal medicine may be added to the culture medium of Aureobasidium pullulans strain. Here, 1 to 4% of glucose is used as a carbon source, preferably 1 to 2% is added. 0.1 to 1% of peptone was added and preferably 0.2 to 0.5% was further added. The culture was carried out using a 5 L fermenter at a temperature of 20 to 40 ° C and for 1 to 7 days while maintaining the pH at 5-7. After culturing, the culture was centrifuged to remove the cultures first.

The herbal medicine to be used in the present invention is not limited to the above-mentioned seven, and other objects and advantages of the present invention will become more apparent from the following detailed description and claims.

Preferably, the extraction is carried out in the presence of an extraction solvent selected from the group consisting of purified water, methanol, ethanol, glycerin, ethyl acetate, butylene glycol, propylene glycol, dichloromethane, chloroform, ethyl ether, butylene glycol, hexane, , More preferably ethanol, 70% ethanol aqueous solution or water, and most preferably 70% ethanol aqueous solution.

According to one embodiment of the present invention, a 70% (v / v) ethanol aqueous solution is added to the herbal fermented product, refluxed three times for 5 hours each, refluxed, filtered with Whatman # 2 filter paper, Can be extracted. When the filtration is completed, the extract solution is transferred to a concentration tank, concentrated under reduced pressure, and lyophilized. Concentration under reduced pressure can be carried out using a rotary vacuum concentrator. Concentration conditions can be obtained by adding 700 ml of the extract to a 1,000 ml round-bottomed flask and concentrating under reduced pressure at a temperature of from 650 to 1,200 rpm at a temperature of from 650 to 1,200 rpm at a pressure of from 650 mmHg to 750 mmHg. The concentrate under reduced pressure was preliminarily frozen to reduce the temperature to -40 ° C, keep it at -40 ° C for 2 to 4 hours, drop to -80 ° C through trap freezing, maintain the vacuum at 10 to 30 mTorr Raise to -40 ° C over 120 minutes. It is then held at -20 ° C for 240 minutes, at 0 ° C for 40 minutes, at 15 ° C for 340 minutes, at 30 ° C for 130 minutes, and then at 1300 minutes until completely dry.

In the present invention, the black yeast fermented herbal medicine extract may be an extract obtained by fermenting a herbal medicine with black yeast or a lyophilized powder obtained by vacuum-concentrating the extract, or may be a blend of the powder with an extraction solvent . The herbal medicine includes ginseng, omija, roundworm, hwanggi, licorice, peony, and guinea pig.

The black yeast fermented crude drug extract is not limited to a specific range, but is preferably contained in an amount of 0.0001 to 30% by weight based on the total weight of the cosmetic composition. When the content of the extract is less than 0.0001% by weight, the effect of improving the skin is not exhibited. When the content of the extract is more than 30% by weight, the effect of increasing the content of the extract is insignificant and there is a problem in safety and stability.

The ingredients contained in the cosmetic composition of the present invention include, in addition to the above-mentioned effective ingredients, the ingredients conventionally used in cosmetic compositions, such as antioxidants, stabilizers, solubilizers, vitamins, And a carrier.

The cosmetic composition of the present invention can be prepared into any of the formulations conventionally produced in the art and can be used in the form of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, , Oil, powder foundation, emulsion foundation, wax foundation and spray, but is not limited thereto.

More specifically, it can be manufactured in the form of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder.

When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

In the case where the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component is selected from aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters.

When the cosmetic composition of the present invention is a soap, a surfactant-containing cleansing agent, or a surfactant-free cleansing agent, it may be applied to the skin and then wiped off or removed or washed with water. The surfactant-containing cleansing formulation is a cleansing foam, a cleansing water, a cleansing towel, and a cleansing pack. The surfactant-free cleansing formulation may be a cleansing cream, , Cleansing lotion, cleansing water and cleansing gel, but is not limited thereto.

In addition, the present invention can provide skin cell proliferation effect, skin aging prevention effect and skin moisturizing effect by using the cosmetic composition comprising the black yeast fermented herbal extract extract.

Hereinafter, the present invention will be described in detail by way of examples, but the present invention is not limited thereto.

Example  One: Black yeast  Manufacture of fermented herbal medicine extract

Ginseng, omija, roundworm, hwanggi, licorice, peony, and guinea were prepared with the same weight, and the sample was selected and thoroughly washed, and the pulverized product was made fine so as to pass through a 300 mesh sieve.

The pulverized product was added to the black yeast strain culture solution so as to have a concentration of 10 g / L. 2% (W / V) of glucose was added as a carbon source and 0.5% (w / v) of peptone was further added thereto. The cultures were incubated for 5 days at 37 ° C and pH 6 using a 5 L fermenter. After culturing the microorganism, the culture was centrifuged and the culture was firstly removed. A 70% (v / v) aqueous ethanol solution was added to the herbal fermentation product, which had been firstly removed from the culture, followed by refluxing three times for 5 hours, followed by cooling and filtration through Whatman # 2 filter paper. After the filtration was completed, the extract was transferred to a thickener, concentrated under reduced pressure at 50 ° C or below, and freeze-dried. The lyophilized product was dissolved in 2% of 50% glycerin to prepare a black yeast fermented herbal medicine extract.

Comparative Example  1: Preparation of herbal medicine extract

Comparative Example 1 is a general organic solvent extract of a herbal medicine, and ginseng, omija, dongle, hwanggi, licorice, peony and persimmon were prepared by the same weight, washed thoroughly and then pulverized. . Ethanol was added to make a final concentration of 70% (v / v) ethanol aqueous solution, refluxed three times for 5 hours, cooled down, and filtered through a filter of whatman # 2. The filtered extract was concentrated under reduced pressure and lyophilized. This powder was dissolved in 50% glycerin to a concentration of 2% (W / V) to prepare herbal medicine extract.

Experimental Example  One: Black yeast  Antioxidative effect of fermented herbal medicine extract

In Experimental Example 1, the antioxidative effect (free radical scavenging ratio) was measured using the DPPH method in order to measure the antioxidative effect of the herbal medicine extracts of Example 1 and Comparative Example 1.

The DPPH method measures the antioxidative effect by reducing power using a free radical called DPPH (2,2-Di (4-tert-octylphenyl) -1-picrylhydrazyl) free radical. Free radical scavenging rate (%) was measured at a wavelength of 560 nm by comparing the degree of decrease in absorbance of DPPH by the test substance (herbal medicine extract) with the absorbance of the blank test solution. As a reagent used, 61.88 mg of a 0.1 mM solution of 2,2-Di (4-tert-octylphenyl) -1-picrylhydrazyl free radical (Aldrich Chem. Co., MW = 618.76) was dissolved in methanol to make 100 ml.

As a measuring method

(1) Add 0.15 ml of the sample solution to 0.15 ml of 0.1 mM DPPH solution in a 96-well plate, stir rapidly, and start culturing at 25 ° C for 10 minutes.

② Measure the absorbance St at 560 nm thereafter.

(3) The blank of the sample solution is measured by the same procedure as that for measuring the absorbance St except that methanol is used instead of the 0.1 mM DPPH solution.

(4) The absorbance Bt is measured by operating in the same manner as in the measurement of the absorbance St except that distilled water is used instead of the sample solution.

(5) Blank of the blank test is carried out in the same manner as in the measurement of the absorbance St except that methanol is used instead of the 0.1 mM DPPH solution and distilled water is used instead of the sample solution, and the absorbance Bo is measured.

The results of the free radical scavenging ratio (%) were calculated according to Equation (1), and the results are shown in FIG. 1 below.

St: absorbance at 560 nm after free radical scavenging of the sample

Bt: absorbance at 560 nm after free radical scavenging of the blank test solution

So: the absorbance at 560 nm before the reaction in the absence of the free radical of the sample

Bo: absorbance at 560 nm before the reaction in the absence of the free radical of the blank test solution

1, it was confirmed that the antioxidative effect of the black yeast fermented crude extract (Example 1), which was extracted after fermentation of the crude drug with the black yeast, was greatly increased as compared with the crude drug extract of Comparative Example 1.

As a control, L-ascorbic acid was used, and it was confirmed that Example 1 of the present invention had an antioxidative effect about 10 times higher than that of Comparative Example 1.

Experimental Example  2: cell activity effect

In order to confirm the cell activity of the black yeast fermented herbal extract obtained in Example 1 of the present invention, the cell proliferation rate of the crude herbal extract of Comparative Example 1 was compared and shown in FIG.

Human normal fibroblasts were inoculated into each well of a 96-well microplate to a concentration of 1 × 10 ⁴ cells and cultured in DMEM medium for 24 hours. After the incubation, the herbal medicine extracts of Example 1 and Comparative Example 1 were dissolved in 1,3-butylene cresol to prepare samples with concentrations (0.05%, 0.1%, 0.2%) and replaced with serum-free DMEM medium. Lt; / RTI > MTT solution 10 μl of dimethylsulfoxide solution [3- (4,5-dimethylazol-2-yl) 2,5-diphenyltetrazolium bromide: 5 mg / ml] was added and stirred for 20 minutes, absorbance at 570 nm. The same procedure was repeated three times.

The cell proliferation rate was calculated by the following equation (2), and the result is shown in FIG.

In Fig. 2, the cell growth rates of Example 1 and Comparative Example 1 were compared, showing that Example 1 extracted after fermentation with black yeast showed about 20% more cell active effect than Comparative Example 1 using general extraction method .

Experimental Example  3: cell migration effect

Analysis of the promoting effect of migration of fibroblasts is one of the common methods of measuring the wound healing effect of cells. Fibroblast HDFn cells were cultured in a 24-well plate, and then cells were scraped in vitro using microtips (200 μl), and the herbal extract extracts of Comparative Example 1 and Example 1 were added at 0.1%, 0.2% Cells were treated with 0.5% and 1.0% concentrations, and reacted for 24 hours. After 24 hours, the cells migrated under the microscope were photographed and the density of cells transferred to the wounding location was determined.

Fig. 3 shows the cell migration effect of the black yeast fermented herbal medicine extract obtained in Example 1, in comparison with the herbal medicine extract of Comparative Example 1. Fig. As a result of the evaluation of mobility in HDFn cells, it can be seen that Example 1, which is a herbal medicine extract extracted by fermentation with black yeast, shows a superior cell migration effect as compared with Comparative Example 1 which is a general extract. 3, when the extract of Example 1 was treated after 24 hours, it was confirmed that the cell migration was actively performed as compared with the case of treating the extract of Comparative Example 1 with little empty space depending on the concentration .

Experimental Example  4: Skin moisturizing effect

In order to confirm the skin moisturizing effect of the black yeast fermented herbal medicine extract obtained in Example 1 of the present invention, a cosmetic (Formulation Example 1) containing a composition containing the black yeast fermented herbal extract was prepared, and skin moisturizing enhancing effect The following experiment was carried out. In this case, Comparative Formulation Example 1 containing the herbal extract of Comparative Example 1 and Comparative Example 2 of the control without the extract were prepared instead of the black yeast fermented herbal extract, and the skin moisturizing effect was compared.

The experiment was divided into two groups of 20 persons to 20 persons per group of 20 to 40 persons without skin disease, and the cream of Formulation Example 1 and Comparative Formulations 1 and 2 among the cosmetic materials prepared in the following Table 1 Twice a day for one month. The skin conductivity was measured by a corneometer (CM820 courage Khazaka electronic GmbH, Germany) in a constant temperature and humidity condition (24 ° C, 40% humidity) before the start of application. The skin conductivity after the passage of time was measured and the rate of increase was evaluated. The results are shown in Table 2 below.

(Unit: wt%) Raw material Formulation Example 1 Comparative Formulation Example 1 Comparative Formulation Example 2


One


Stearyl alcohol 8 8 8 Stearic acid 2 2 2 Stearic acid 2 2 2 Squalane 4 4 4 2-octyldodecyl alcohol 6 6 6 Polyoxyethylene (25 mole addition) alcohol ester 3 3 3 Glyceryl monostearate Aster 2 2 2 2 Propylene glycol 5 5 5
3

Black yeast fermented herbal medicine extract
(Example 1) 3 - - Herbal extract extract
(Comparative Example 1) - 3 - 4 Purified water to 100 to 100 to 100

Skin Conductivity Growth Rate (%) sample After 1 week after 2 weeks After 4 weeks Formulation Example 1 42 45 47 Comparative Formulation Example 1 33 35 38 Comparative Formulation Example 2 25 25 26

As shown in the results of Table 2, in the case of Formulation 1 containing the black yeast fermented herbal extract of the present invention, the rate of skin conductivity increase was much higher than that of Comparative Formulations 1 and 2. In addition, in general, the skin conductivity is proportional to the skin moisture content. From the above results, it can be seen that Formulation Example 1 according to the present invention maintains the skin moisture content higher than Comparative Formulation Examples 1 and 2. As a result, it was found that the herbal extracts were effective for skin moisturizing and the extracts obtained by fermenting herbal medicine with black yeast proved to be the most effective.

Experimental Example  5: Evaluation of skin elasticity improvement effect

The skin elasticity improvement effect of the cream containing the black yeast fermented herbal extract (Formulation Example 1) and Comparative Formulation Example 1 and Comparative Formulation Example 2 cream was evaluated as follows.

In 15 subjects of the experiment (20 to 35 year old), the cream of Formulation Example 1 was applied to the right side of the face, the cream of Comparative Formulation Example 1 and the cream of Comparative Formulation Example 2 were applied to the left side of the face for 2 consecutive months Respectively.

The skin elasticity was measured by using a skin elasticity meter (SEM 575, C + K Electronic Co., Germany) before and after using the product. The results are shown in the following Table 3 as ΔR8 values of a skin elasticity meter (Cutometer SEM 575), wherein the R8 value indicates the viscoelasticity of the skin.

As shown in the following Table 3, it can be seen that the skin elasticity improving effect of the creamer coated with the black yeast fermented herbal medicine extract is the best.

Experimental product
Skin elasticity effect (ΔR8) Before use after use Formulation Example 1 0.12 0.30 Comparative Formulation Example 1 0.12 0.23 Comparative Formulation Example 2 0.11 0.18

n = 15, p < 0.05

Claims (7)

A cosmetic composition comprising a herbal medicine extract fermented with Aureobasidium pullulans , wherein the herbal medicine is ginseng, omija, orientale, hwanggi, licorice, peony, and guinea pig. The cosmetic composition according to claim 1, wherein the cosmetic composition is for skin cell proliferation. The cosmetic composition according to claim 1, wherein the cosmetic composition is for preventing skin aging. The cosmetic composition according to claim 1, wherein the cosmetic composition is for skin moisturizing. The fermentation method according to any one of claims 1 to 4, wherein the fermentation is carried out in the presence of ginseng, omija, roundworm, ylang-yu, licorice, peony, and rhubarb as black fungi at a temperature of 20 to 40 DEG C , And fermented for 1 to 7 days. 5. The process according to any one of claims 1 to 4, wherein the extraction is carried out in the presence of a diluent selected from the group consisting of purified water, methanol, ethanol, glycerin, ethyl acetate, butylene glycol, propylene glycol, dichloromethane, chloroform, ethyl ether, butylene glycol, And a solvent selected from the group consisting of a mixture of water and an organic solvent. The cosmetic composition according to any one of claims 1 to 4, wherein the black yeast fermented herbal extract is contained in an amount of 0.0001 to 30.0% by weight based on the total weight of the cosmetic composition.

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