KR20200082812A - Cosmetic and pharmaceutical compositin comprising aloe extract, upland rice extract and ceramide - Google Patents

Abstract

The present invention relates to a cosmetic and pharmaceutical composition comprising a natural extract and ceramide. More specifically, provided is a cosmetic and pharmaceutical composition which can be useful for preventing, alleviating, and treating skin diseases by containing a mixed composition containing an aloe extract, a mountain rice extract, and ceramide as an active ingredient, thereby exhibiting excellent skin moisturizing effects, skin barrier strengthening effects and atopy relieving effects.

Description

Cosmetics and pharmaceutical composition comprising aloe extract, field rice extract and ceramide {COSMETIC AND PHARMACEUTICAL COMPOSITIN COMPRISING ALOE EXTRACT, UPLAND RICE EXTRACT AND CERAMIDE}

The present invention relates to a cosmetic and pharmaceutical composition comprising a natural extract and ceramide, and more specifically, a mixture composition comprising an aloe extract, a paddy rice extract and ceramide as an active ingredient for prevention, improvement and treatment of skin diseases It relates to a cosmetic composition and a pharmaceutical composition that can be useful as.

The skin carries about 65-70% of the body's moisture, that is, various physiologically active substances necessary for the human body, and plays an important role in controlling the evaporation of this moisture outside the body, which helps the skin to maintain a soft and moist state. Do it.

These skins are largely divided into three layers of epidermis, dermis, and subcutaneous fat tissue from the outside. In particular, the epidermis located at the outermost part of the skin blocks excessive penetration of substances from the outside and excessive moisture is excreted from the body. It performs a protective function to suppress evaporation.

This protective function is possible by forming and maintaining a stratum corneum composed of keratinocytes normally.

Keratinocytes are cells formed by undergoing morphological and functional changes step by step as the basal cells, which were continuously proliferating in the stratum basale, migrate to the stratum corneum. The forming cells are removed from the skin, and new keratinocytes from the lowermost layer of the epidermis repeat the process of epidermis differentiation or keratinization, which replaces its function. In this keratinization process, keratinocytes produce a high concentration of natural moisturizing factor (NMF), a water-soluble component, and intercellular lipids such as ceramide, cholesterol, and fatty acids, so that the stratum corneum acts as a barrier layer to the skin. By retaining the function as a skin barrier, the skin acts to exhibit flexibility, as well as maintaining adequate moisture.

However, skin dryness, which is considered to be one of the major diseases in modern society, is one of the symptoms caused by abnormal skin barrier function, artificial temperature control of heating/cooling according to changes in the environment or changes in life patterns, and various kinds that occur in social life. Due to various causes such as skin stress due to stress and environmental pollution, frequent washing according to makeup habits, and natural skin aging due to age increase, the moisture in the stratum corneum decreases, the skin becomes dry, the surface becomes rough, and the skin loses its luster. Because of the dull-looking phenomenon, the need for skin moisturizers is increasing.

In addition, atopic dermatitis, which occurs in 10% of children, is also known to be a major cause of skin dryness and, more fundamentally, an abnormality of the skin barrier function.

Currently, as a prescription for atopic dermatitis, drugs such as steroids, antihistamines, and antibiotics are predominantly prescribed, but they cause various problems such as skin weakness and hormonal abnormalities.

Accordingly, research is underway to treat and alleviate atopic symptoms that are safe for humans from natural products, and Korean Patent Registration No. 10-1924054 contains a new compound stechamon separated from the bloodroot extract, as an active ingredient, for improving atopic dermatitis This is described, and Korean Patent Registration No. 10-0990195 contains allergies including aroma essential oil (at least one of epidermis extract, bellflower extract, lavender, tea tree, blue chamomile, white chamomile, frankinense, sandalwood) and hyaluronic acid , Atopic skin disease relief natural cosmetic composition is disclosed.

However, atopic dermatitis has different symptoms depending on chemokine concentration, quantitative change, or activity level, and can be seen as a phenomenon in which proliferation of keratinocytes is abnormally caused by immunological factors. It occurs as a complex factor between factors and has limitations in finding and treating its origin.

Korean Registered Patent No. 10-1924054 Korean Registered Patent No. 10-0990195

The present invention is intended to solve the problems as described above, by containing a mixed composition containing an aloe extract, field rice extract and ceramide as an active ingredient, cosmetics and pharmaceuticals useful for the prevention, improvement and treatment of skin diseases It is an object to provide a composition.

According to an aspect of the present invention, there is provided a cosmetic composition comprising an aloe extract, a paddy rice extract and a mixed composition comprising ceramide as an active ingredient.

The mixed composition may provide a cosmetic composition comprising 20 to 300 parts by weight of the paddy rice extract with respect to 100 parts by weight of the aloe extract.

The mixed composition may provide a cosmetic composition comprising 20 to 300 parts by weight of the ceramide with respect to 100 parts by weight of the aloe extract.

The mixed composition may provide a cosmetic composition, characterized in that it further comprises 30 to 200 parts by weight of Mojaban extract relative to 100 parts by weight of the aloe extract.

It is possible to provide a cosmetic composition characterized in that the ceramide is pseudo-ceramide.

The pseudo-ceramide may provide a cosmetic composition characterized by being one of the following compounds.

[Compound 1]

[Compound 2]

[Compound 3]

The cosmetic composition may provide a cosmetic composition for moisturizing the skin, strengthening the skin barrier, or improving atopy.

According to another aspect of the present invention, there is provided a pharmaceutical composition for preventing, improving, or treating atopy containing an aloe extract, a paddy rice extract, and a mixed composition comprising ceramide as an active ingredient.

The mixed composition may provide a pharmaceutical composition for preventing, improving, or treating atopic dermatitis, which further comprises a capsicum extract.

According to the cosmetic and pharmaceutical composition containing the mixed composition comprising the aloe extract, field rice extract and ceramide according to the present invention, the skin moisturizing effect, skin barrier strengthening effect and atopy improving effect are excellent, thus preventing, improving and treating skin diseases It is useful as a dragon.

Hereinafter, embodiments of the present invention will be described in detail. However, this is presented as an example, and the present invention is not limited thereby, and the present invention is only defined by the scope of claims to be described later.

In describing the present invention, when it is determined that detailed descriptions of related known configurations or functions may obscure the subject matter of the present invention, detailed descriptions thereof will be omitted.

In describing the components of the present invention, terms such as first, second, A, B, (a), and (b) may be used. These terms are only for distinguishing the component from other components, and the nature, order, or order of the component is not limited by the term.

In describing the present invention, the terms'about','substantially','degree', etc. of degree are used in or close to the value when manufacturing and substance tolerances unique to the stated meaning are presented. In order to aid the understanding of the present invention, accurate or absolute values are used to prevent unconscionable infringers from using the disclosed disclosure.

In the present invention, the extract includes all of the extracts, fractions and refinements, their diluents, concentrates or dried products obtained at each stage of extraction, fractionation or purification.

In the present invention, the terms'prevention','improvement' or'treatment' means suppression, alleviation or elimination of the appearance or development of one or more symptoms associated with a disease or disorder.

The cosmetic composition according to an embodiment of the present invention may contain a mixed composition comprising aloe extract, field rice extract and ceramide.

Aloe (aloe) is a perennial herbaceous perennial plant that is currently used as a medicinal plant worldwide. It is similar in appearance to a cactus, but actually belongs to the lily family and the aloe genus. It accounts for about a tenth of the family. Aloe, originated in Africa's warm, dry climate, was used by ancient Egyptians to treat skin wounds, burns, and inflammations. In modern times, it was often used in hospitals for light skin disease treatment and radiation dermatitis in the early 1930s. It has been loved by many for healing wounds since the Greek era when medicine was not developed, and it has been used for a long time since ancient times as a cosmetic ingredient for skin.

Aloe has a slight difference depending on the type, but it has a spear-like shape with thorns on both ends of the green leaves, and the ends of the leaves are pointed. The main components of aloe are polymer polysaccharides containing hexose, aloetin, aloe ulcin, and alomitine, which are mostly present in the gel inside the leaves.

Aloe is widely known as a plant that moisturizes the skin, and has an excellent skin moisturizing effect, making it a moisture barrier for soft baby skin. In addition, it has an excellent effect on wound healing and cell regeneration, and the main ingredient, aloetin, has an effect on skin sterilization and thus an effect on prevention of skin trouble.

The aloe extract can be obtained by using water, ethanol, or a mixture of water and ethanol as an extraction solvent, and drying and pulverizing the aloe to obtain a mixture of purified water and 30 to 70% ethanol as an extraction solvent.

Upland rice is rice grown in a field, and unlike paddy rice grown in paddy fields grown during the entire growing season, it grows well in a field that has not been watered to withstand drought. In the report on paddy rice and paddy rice published by the Rural Development Administration, the differences between paddy rice and paddy rice are variously described. Among them, to briefly explain the difference between paddy rice and paddy rice, paddy rice has a slightly thicker leaf and fewer pores than the paddy rice, and the opening and closing functions of pores are more sensitive than paddy rice. This thick and large one has the advantage of high water storage capacity, and it is described that the thick root and the large size of the water pipe and the large size of the water pipe are related to its own water supply capacity.

In addition, it has been described that field rice is more resistant to calcium chlorate (KClO ₃ ) than paddy rice, and the strength of drought resistance in young mothers among the field rice varieties is due to the strong resistance to calcium chlorate. That is, since there are various differences between field rice and paddy rice due to breeding, it is judged that the moisturizing effect will be different. On the other hand, the field rice can be used for harvesting native to Jeju Island, but is not limited or limited thereto.

The field rice extract may be obtained by extracting at one or more sites among outpost, root, leaf, stem, and Chongqing.

The paddy rice extract can be obtained by using water, ethanol, or a mixture of water and ethanol as an extraction solvent, and then drying and grinding the paddy rice to obtain a mixture of purified water and 30 to 70% ethanol as an extraction solvent.

The mixed composition may include 20 to 300 parts by weight of the paddy rice extract relative to 100 parts by weight of the aloe extract, preferably 50 to 200 parts by weight. In the mixed composition, when the field rice extract is less than 20 parts by weight relative to 100 parts by weight of the aloe extract, there may be a slight problem in efficacy and effectiveness, and when it exceeds 300 parts by weight, it is inefficient to promote efficacy and effectiveness compared to the amount used, which is economical There may be a problem.

Ceramide is a major lipid component of the stratum corneum and plays an important role in the formation of moisturizing ability of skin and the development of skin barrier diseases. Ceramide is a type of sphingo lipid that has a structure in which fatty acids are linked to sphingosine or phytosphingosine.It is composed of stratum corneum intercellular lipids that make up the stratum corneum of the skin. As a component, it functions as a lipid barrier that suppresses evaporation of water in the stratum corneum and maintains the square structure of the stratum corneum. Ceramides present in the human body are classified into six types according to the degree of polarity. Ceramide IOP (Ceramide 1), Ceramide NS, and Ceramide Engine (Ceramide 2) have the lowest polarity and sphingosine as a skeleton, and Ceramide NP (Ceramide 3) is a synthetic N-acetylated phytosphingosine mainly having an erythric structure. to be. Because of each of these characteristic structural and physicochemical properties, ceramide plays a role in forming an optimal transmission barrier.

In the present invention, the ceramide may use the above-described ceramide, and similar ceramide may be used.

The pseudo ceramide may be one of the following compounds, but is not limited thereto.

[Compound 1]

[Compound 2]

[Compound 3]

The mixed composition may include 20 to 300 parts by weight of the ceramide with respect to 100 parts by weight of the aloe extract, preferably 50 to 200 parts by weight. If the ceramide is less than 20 parts by weight with respect to 100 parts by weight of the aloe extract in the mixed composition, there may be a slight problem in efficacy and effectiveness, and when it exceeds 300 parts by weight, it is inefficient in promoting efficacy and effectiveness compared to the amount used, which is an economical problem. There may be.

The mixed composition comprising the aloe extract, field rice extract, and ceramide may further include a capsicum extract.

Sargassum fulvellum is apparently distinct from the root, stem, and leaf, and the root is gaban-shaped and grows 1 to 3 m or more by cutting out one central branch. The stem is triangular or triangular and twisted. The leaves are curved toward the base from the stem, curved, and have a spatula shape or oval shape, and the middle part of the leaf is formed. The upper leaves are lanceolate and have serrated projections on the edges, and air bubbles are formed from the stems all over the body. It is dark yellow-brown and can be seen all over Korea.

The main ingredients are alginic acid, chlorophyll a (c), β-carotene, fucoxanthin, laminarin, and mannitol, and the diet is analyzed when it is analyzed to fly in cap. The amount of fiber and fat is very low, but when analyzed in powder form, the amount of fiber and fat is more than 30%.

Mopan is relatively high in dietary fiber than in sugar. In Korea, many types of caps are usually edible, and are used as raw materials in the seaweed industry such as alginic acid or as fertilizer. It is said that alginic acid helps digestion of the stomach and helps intestinal movement, which is good for constipation and effective in preventing colon cancer. It is good for hypertension and osteoporosis due to its high mineral content and good for thyroid dysfunction due to its high iodine content.

The hatban extract can be obtained by using water, ethanol, or a mixture of water and ethanol as an extraction solvent, and drying and grinding the hatban to obtain a mixture of purified water and 30 to 70% ethanol as an extraction solvent.

The mixed composition may include 30 to 200 parts by weight of the capsicum extract with respect to 100 parts by weight of the aloe extract, preferably 50 to 100 parts by weight. In the mixed composition, when the capsicum extract is less than 30 parts by weight with respect to 100 parts by weight of the aloe extract, there may be a slight problem in efficacy and effectiveness. There may be a problem.

In the present invention, the mixed composition includes all of a mixture comprising an aloe extract, a field rice extract and ceramide, and a mixture further comprising a capsicum extract in the mixture.

In the present invention, the cosmetic composition containing the mixed composition as an active ingredient may be used for moisturizing the skin, strengthening the skin barrier, or improving atopy.

The skin moisturizing refers to the effect of suppressing moisture loss and supplying moisture to the skin so that moisture does not quickly disappear on the skin and stays on the skin for a long time.

Reinforcing the skin barrier means enhancing the barrier function of the stratum corneum located at the outermost surface of the skin. The stratum corneum, the primary protective layer of the skin, can be damaged by physical irritation or wounds. In the case of a person with atopic dermatitis or dry skin, the function of the skin barrier of the stratum corneum is improper or damaged. So-called moisturizers are applied to reduce the treatment or damage to atopy or dry skin, and general moisturizers cannot solve fundamental problems by simply replenishing moisture or covering the skin. Therefore, in this aspect, the mixed composition enhances the skin barrier function by solving the fundamental problem by increasing the expression of factors related to the barrier function of the stratum corneum.

In the present invention, the cosmetic composition contains a cosmetic or dermatologically acceptable medium or base. This is any formulation suitable for topical application, for example, can be formulated as one selected from the group consisting of lotion, emulsion, gel, cream, lotion, essence, foam, pack, soap, ointment, spray and powder. It is not.

In the present invention, the cosmetic composition may include components commonly used in cosmetic compositions in addition to the mixed composition. For example, fatty substances, organic solvents, solubilizers, thickeners, gelling agents, emollients, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, surfactants, water, ionic or nonionic emulsifiers, Cosmetics or skin, such as fillers, metal ion blockers, chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic actives, lipid vesicles or any other ingredient commonly used in cosmetics. It may contain adjuvants commonly used in science. The adjuvant is introduced in an amount commonly used in the cosmetic or dermatological field.

In the present invention, the active ingredient of the mixed composition is 0.001 to 50% by weight, preferably 0.05 to 20% by weight, preferably 0.1 to 10% by weight based on the total cosmetic composition. If the weight of the mixed composition is less than 0.001% by weight, efficacy and effectiveness may be insignificant, and if it exceeds 50% by weight, there may be skin stability or formulation problems.

The pharmaceutical composition according to another embodiment of the present invention may include the mixed composition as an active ingredient. The pharmaceutical composition may be used for preventing, improving or treating atopy. The pharmaceutical composition may further contain a pharmaceutical adjuvant and other therapeutically useful substances such as a preservative, stabilizer, hydration or emulsifying accelerator, salt and/or buffer for osmotic pressure control. The pharmaceutical composition may be formulated as a lotion, cream, ointment, gel, or the like, but is not limited thereto.

In the present invention, the active ingredient of the mixed composition is 0.001 to 50% by weight, preferably 0.05 to 20% by weight, preferably 0.1 to 10% by weight based on the total pharmaceutical composition. If the weight of the mixed composition is less than 0.001% by weight, efficacy and effectiveness may be insignificant, and if it exceeds 50% by weight, there may be skin stability or formulation problems.

Hereinafter, the present invention will be further described through production examples and test examples. These examples are intended to illustrate the present invention more specifically, but the scope of the present invention is not limited to these examples.

[ Example ]

[ Manufacturing example ]

[Production Example 1] Preparation of individual extracts

Aloe, Jeju paddy, and Mojaban were each washed with distilled water, dried and pulverized with a blender to obtain a fine powder sample. Each extract was prepared by adding twice the volume of the solvent mixed with purified water and ethanol in a 5:5 ratio to about 10 times the volume of each micropowder sample, followed by extraction twice. After performing the extraction, filtered through a 400 mesh filter cloth, and the obtained filtrate was concentrated using a vacuum concentrator to obtain individual extract powders.

[Production Example 2] Preparation of compound 3

To the reaction flask, 0.9 g of sulfinol and 2 ml of triethylethylamine were added, 3 ml of methanol and 20 ml of dichloromethane were added, and the temperature was lowered to a low temperature (about 5°C). A solution of α-linolenic acid chloride (3.0 g) and dichloromethane (20 ml) was added dropwise to the reaction flask. The reaction solution was stirred at room temperature for 6 hours, and then the solution was concentrated under reduced pressure. The concentrated solution was dissolved in dichloromethane (30 ml), washed with 0.5N hydrochloric acid aqueous solution, saturated aqueous sodium hydrogen carbonate solution, and water. The organic solution layer was dried over anhydrous magnesium sulfate, filtered, and the solution was concentrated under reduced pressure and recrystallized to obtain 2.9 g (82%) of a pale yellow solid and yellow solid.

¹ H NMR (400MHz, DMSO-d ₆ ) δ 7.41 (d, J = 8 Hz, 1H), 5.36-5.30 (m, 6H), 4.57-4.54 (m, 2H), 3.70-3.69 (m, 1H) , 3.38-3.32 (m, 4H), 2.79-2.37 (m, 4H), 2.08-2.02 (m, 6H), 1.48-1.45 (m, 2H), 1.30-1.26 (m, 6H), 0.93 (t, J = 7.6 Hz, 3H).; ¹³ C NMR (100MHz, CDCl ₃ ) δ 172.53, 131.95, 130.37, 130.19, 128.39, 128.38, 128.20, 128.00, 127.44, 60.67, 53.18, 35.88, 31.36, 29.51, 29.18, 29.14, 29.06, 27.11, 27.06, 25.79, 25.66, 25.57, 22.44, 20.50, 14.58, 14.39.; FT-IR (Neat) 3300 cm ^-1 , 2928 cm ^-1 , 2853 cm ^-1 , 1641 cm ^-1 , 1548 cm ^-1 , 1467cm ^-1 , 1424 cm ^-1 , 1387 cm ^-1 , 1248 cm ^-1 , 1073 cm ^-1 , 1058 cm ^-1 .; MS (ESI): m/z = 374.27 [M+Na] ⁺ .

[Production Example 3] Preparation of composition

Compositions according to Examples and Comparative Examples were prepared with the composition shown in Table 1 below using the aloe extract, Jeju paddy rice extract, and capsicum extract prepared in Preparation Example 1 and Compound 3 synthesized in Preparation Example 2.

Aloe
extract Rice paddy
extract Hat half
extract Ceramide 2 (NG) Ceramide 3 (NP) Compound 3 Untreated - - - - - - Comparative Example 1 One - - - - - Comparative Example 2 - One - - - - Comparative Example 3 - - - - - One Comparative Example 4 - - One - - - Comparative Example 5 One One - - - - Comparative Example 6 One One One - - - Example 1 One One - One - - Example 2 One 2 - - One - Example 3 One One - - - One Example 4 One One - - - 2 Example 5 One One One - - 0.5 Example 6 One 0.5 0.5 - - 2 Example 7 One One One - - One

(Unit: parts by weight)

[ Test example ]

[Test Example 1] Skin keratinocytes culture

The skin keratinocytes, HaCaT cells, were Pre-sale from CG Hyun (Jeju National University, Korea) using a Dulbecco's Modified Eagle's Medium (DMEM) medium containing 100 units/ml penicillin-streptomycin and 10% fetal bovine serum (FBS) at 37°C, 5% CO ₂ incubator Cultured at 3-4 days intervals.

[Test Example 2] Cytotoxicity evaluation (EZ-cytox assay)

The EZ-cytox assay is a representative method of measuring cell viability using the principle that water solution tetrazolium salt (WST) reacts with dehydrogenase of living cells to produce an orange water-soluble formazan.

In order to confirm the toxicity in keratinocytes, HaCaT cells were dispensed into 96 well plates at 5 Х 10 ⁴ cells/mL using DMEM medium with 10% FBS added for 18 hours at 37°C and 5% CO ₂ conditions. Cultured for a while. The cultured HaCaT cells were exchanged with serum-free DMEM to treat the extract to be evaluated. Thereafter, EZ-cytox was added to each well and reacted at 37°C and 5% CO ₂ for 30 minutes, and absorbance at 450 nm was measured using a microplate reader. Average absorbance values were obtained for each sample group, and cell viability was evaluated by comparing with the absorbance values of the control group.

division Treatment concentration (μg/mL) Cytotoxic HaCaT cell viability (%) Untreated - 100 Comparative Example 1 100 103 ± 2.1 Comparative Example 2 100 101 ± 1.2 Comparative Example 3 100 103 ± 1.3 Comparative Example 4 100 101 ± 2.4 Comparative Example 5 100 101 ± 4.1 Comparative Example 6 100 101 ± 1.1 Example 1 100 104 ± 1.1 Example 2 100 105 ± 3.2 Example 3 100 101 ± 4.1 Example 4 100 100 ± 1.3 Example 5 100 102 ± 2.2 Example 6 100 103 ± 3.1 Example 7 100 103 ± 4.3

As shown in Table 2, in Comparative Examples 1 to 6 and compositions 1 to 7, which were compositions containing ceramides according to the present invention, cytotoxicity was not observed in keratinocytes and macrophages.

[Test Example 3] Moisturizing effect

[Test Example 3-1] Hyaluronic acid (Hyaluronic Acid) production increase effect confirmed

HaCaT cells were divided into 1.0 Х 10 ⁵ cells/mL in 24 well plates and cultured for 18 hours at 37°C and 5% CO ₂ conditions. The cells were exchanged with Serum-free DMEM, and the composition prepared by Table 1 was treated to incubate for 24 hours. After that, the culture medium was collected, centrifuged at 15,000 rpm for 5 minutes, and the supernatant was taken out and stored frozen (-20°C) until quantification. The enzyme immunoassay (ELISA: Enzyme-Linked Immunosorbent Assay) was used as a hyaluronic acid ELISA kit (Elabscience Biotechnology Co., Ltd) and was performed by the method provided by the manufacturer.

Treatment concentration (μg/mL) Hyaluronic Acid Production (%) Untreated - 100 Comparative Example 1 100 181 ± 2.8 Comparative Example 2 100 120 ± 2.1 Comparative Example 3 100 165 ± 5.6 Comparative Example 4 100 130 ± 3.6 Comparative Example 5 100 201 ± 6.1 Comparative Example 6 100 215 ± 2.6 Example 1 100 222 ± 2.2 Example 2 100 226 ± 5.9 Example 3 100 231 ± 6.1 Example 4 100 236 ± 3.3 Example 5 100 248 ± 1.7 Example 6 100 261 ± 4.2 Example 7 100 252 ± 2.2 Retinoic acid 10 243 ± 0.6

As shown in Table 3, when the Examples 1 to 7, which are compositions containing the ceramide according to the present invention, were treated, the hyaluronic acid, a skin moisturizing factor, was higher than Comparative Examples 1 to 6. Particularly, the compositions of Examples 5 to 7 including aloe extract, Jeju paddy rice extract, complex extract containing hatban extract, and ceramide had a hyaluronic acid production amount of 248% or higher, which is higher than the control group retinoic acid.

[Test Example 3-2] Moisturizing factor Aquaporin3 (AQP3) confirmed the increase effect of production

HaCaT cells were distributed in 6 well plates at 4.0 Х 10 ⁵ cells/mL and cultured at 37° C. and 5% CO ₂ for 18 hours. The cells were exchanged with Serum-free DMEM, and the composition prepared by Table 1 was treated to incubate for 24 hours. Subsequently, each group was treated with PBS containing no drug that could affect protein quantification after removal of the culture solution and washing with PBS. It was lysed by repeating low temperature and room temperature incubation to collect proteins. Quantitatively, AQP3-ELISA kit (Elabscience Biotechnology Co., Ltd) was used and was performed by the method provided by the manufacturer.

Treatment concentration (μg/mL) Aquaporin3 production (%) Untreated - 100 Comparative Example 1 100 172 ± 5.4 Comparative Example 2 100 135 ± 4.8 Comparative Example 3 100 138 ± 4.3 Comparative Example 4 100 129 ± 3.1 Comparative Example 5 100 192 ± 4.1 Comparative Example 6 100 205 ± 4.5 Example 1 100 236 ± 4.2 Example 2 100 242 ± 6.2 Example 3 100 258 ± 4.2 Example 4 100 272 ± 2.9 Example 5 100 249 ± 2.6 Example 6 100 289 ± 3.3 Example 7 100 271 ± 4.8 Retinoic acid 10 234 ± 3.9

As shown in Table 4, the treatment of Examples 1 to 7, which is a composition containing the ceramide of the present invention, was higher than that of Comparative Examples 1 to 6, and the amount of Aquaporin3, a skin moisturizing factor, was higher. In addition, when looking at the individual extracts Comparative Examples 1 to 3, it was found that aloe was the highest, and the cap was higher than other extracts. In addition, looking at Comparative Examples 1, 5, and 6, aloe extract seems to enhance the effect of containing rice rather than single, and it was confirmed that the amount of Aquaporin3 was further increased when more capsicum was added thereto. Looking at Comparative Example 6 and Example 1, it can be seen that when the aloe and field rice complex extracts causing effect enhancement include more ceramides than capsicum extracts, it exhibits much better efficacy. The complex extract of aloe and paddy rice containing the ceramide of the present invention has the effect of significantly improving the production rate of hyaluronic acid and Aquaporin3, and thus it was confirmed that it is very effective in moisturizing the skin.

[Test Example 4] Skin barrier strengthening

[Test Example 4-1] Related Test 1

HaCaT cells were distributed in 6 well plates at 4.0 Х 10 ⁵ cells/mL and cultured at 37° C. and 5% CO ₂ for 18 hours. The cells were exchanged for Serum-free DMEM, and the composition prepared by Table 1 was treated to incubate for 24 hours. Subsequently, each group was treated with PBS containing no drug that could affect protein quantification after removal of the culture solution and washing with PBS. It was lysed by repeating low temperature and room temperature incubation to collect proteins. Quantitatively, a Filaggrin-ELISA kit (Elabscience Biotechnology Co., Ltd) was used and was performed by the method provided by the manufacturer.

Treatment concentration (μg/mL) Filaggrin production (%) Untreated - 100 Comparative Example 1 100 169 ± 4.9 Comparative Example 2 100 121 ± 3.4 Comparative Example 3 100 177 ± 2.7 Comparative Example 4 100 119 ± 7.1 Comparative Example 5 100 178 ± 5.5 Comparative Example 6 100 182 ± 3.3 Example 1 100 196 ± 2.4 Example 2 100 190 ± 1.8 Example 3 100 201 ± 5.5 Example 4 100 206 ± 6.3 Example 5 100 215 ± 3.9 Example 6 100 259 ± 4.6 Example 7 100 252 ± 5.1 Retinoic acid 10 217 ± 3.8

As shown in Table 5, when Comparative Examples 1 to 6 treated with Examples 1 to 7, which is a composition containing the ceramide of the present invention, promotes expression of filaggrin to prevent skin dryness and to prevent UV rays. It can be seen that the damage to the skin barrier is prevented and the damaged skin barrier can be quickly improved.

[Test Example 4-2] Related Test 2

HaCaT cells were distributed in 6 well plates at 4.0 Х 10 ⁵ cells/mL and cultured at 37° C. and 5% CO ₂ for 18 hours. The cells were exchanged with Serum-free DMEM, and the composition prepared by Table 1 was treated to incubate for 24 hours. Subsequently, each group was treated with PBS containing no drug that could affect protein quantification after removal of the culture solution and washing with PBS. It was lysed by repeating low temperature and room temperature incubation to collect proteins. Quantification was performed using an Involucrin-ELISA kit (Elabscience Biotechnology Co., Ltd) and the method provided by the manufacturer.

Treatment concentration (μg/mL) Involucrin production (%) Untreated - 100 Comparative Example 1 100 181 ± 3.5 Comparative Example 2 100 131 ± 1.2 Comparative Example 3 100 159 ± 3.8 Comparative Example 4 100 119 ± 2.1 Comparative Example 5 100 185 ± 5.2 Comparative Example 6 100 189 ± 7.6 Example 1 100 196 ± 2.4 Example 2 100 203 ± 7.8 Example 3 100 195 ± 5.2 Example 4 100 199 ± 3.1 Example 5 100 232 ± 6.1 Example 6 100 254 ± 4.4 Example 7 100 247 ± 2.8 Retinoic acid 10 228 ± 1.7

As shown in Table 6, when the Examples 1 to 7, which are compositions containing the ceramide of the present invention, were treated than Comparative Examples 1 to 6, the expression of involucrin was promoted to prevent skin dryness and ultraviolet rays. It can be seen that the damage to the skin barrier is prevented and the damaged skin barrier can be quickly improved.

[Test Example 5] Measurement of the inhibitory activity of atopic factors, TARC and MDC

In order to confirm the atopic improvement effect on the composition of the present invention, it was intended to analyze the inhibitory activity of atopic chemokine (TARC: Thymus and activation regulated chemokine, MDC: Macrophage-derived chemokine).

HaCaT cells were divided into 1.0 Х 10 ⁵ cells/mL in 24 well plates and cultured for 18 hours at 37°C and 5% CO ₂ conditions. The mixture was exchanged with Serum-free DMEM, and the composition prepared by Table 1 and interferon-γ (IFN-γ, 10 ng/mL) were treated together to incubate for a period of time. Then, the culture medium was centrifuged (12,000 rpm, 3 min) to measure the atopy chemokine production content of the supernatant obtained. All samples were stored frozen (-20°C) until quantification. For the TARC and MDC contents, a human enzyme-linked immnunosorbent assay (ELISA) kit (R&D Systems Inc., Minneapolis, MN, USA) was used, and the r ² value of the standard curve for the standard was 0.99 or more.

Treatment concentration (μg/mL) TARC generation inhibition (%) MDC inhibition (%) Comparative Example 1 100 13.8 ± 1.9 12.2 ± 2.6 Comparative Example 2 100 ND ND Comparative Example 3 100 21 ± 2.9 25.2 ± 5.3 Comparative Example 4 100 ND ND Comparative Example 5 100 19 ± 2.2 17.9 ± 6.2 Comparative Example 6 100 26.4 ± 7.6 24.9 ± 0.9 Example 1 100 35.3 ± 5.2 38.2 ± 6.1 Example 2 100 38.3 ± 4.8 40.2 ± 8.6 Example 3 100 45.1 ± 2.2 42.1 ± 6.2 Example 4 100 49.9 ± 1.8 45.1 ± 4.3 Example 5 100 51.8 ± 3.0 48.7 ± 5.4 Example 6 100 58.1 ± 5.2 54.2 ± 7.4 Example 7 100 55.8 ± 4.7 53.3 ± 2.1 silymarin 10 52.3 ± 1.9 40.2 ± 2.6

As shown in Table 7, Examples 1 to 7, which are compositions containing the ceramide of the present invention, had a higher inhibition rate of atopic chemokine production than Comparative Examples 1 to 4. In particular, in Comparative Examples 2 and 4, there was no inhibition rate of atopic chemokine production, but as in Comparative Examples 5 and 6, in the case of the complex extracts of aloe and paddy rice, aloe and paddy rice, and complex extracts of motherwort, the inhibitory rate of chemokine production was increased. I was able to confirm. And, it was confirmed through Examples 1 to 7 that ceramide has an effect of further enhancing the efficacy of the aloe and paddy rice complex extract, which was found to be very effective for atopic skin diseases.

Formulation example

[Formulation Example 1] Preparation of flexible lotion

As shown in Table 8 below, a flexible lotion containing the composition of the present invention as an active ingredient was prepared according to a conventional method.

Ingredients Content (% by weight) Example 1 0.5 Butylene glycol 2.0 Propylene glycol 2.0 Acrylate/C10-C30 alkylacrylate crosspolymer 0.1 Polysorbate 80 0.4 Arginic acid 0.1 Xanthan gum 0.1 Hyaluronic acid 1.0 Preservatives, pigments, flavors Proper Purified water Up to 100

[Formulation Example 2] Cream Preparation

As shown in Table 9 below, a cream containing the composition of the present invention as an active ingredient was prepared according to a conventional method.

Ingredients Content (% by weight) Example 3 0.5 Beta-1,3-glucan 5.0 Polysorbate 80 1.5 Squalane 5.0 glycerin 5.0 Butylene glycol 3.0 Propylene glycol 3.0 Cetearyl Olivet/ Sorbitan Olivet 1.0 Preservatives, pigments, flavors Proper Purified water Up to 100

[Formulation Example 3] Skin external preparation

As shown in Table 10 below, an external preparation for skin containing the composition of the present invention as an active ingredient was prepared according to a conventional method.

Ingredients Content (% by weight) Example 7 0.5 Beta-1,3-glucan 5.0 Polysorbate 80 5.0 PEG 60 2.0 Shea butter 5.0 Squalane 5.0 glycerin 10.0 Propylene glycol 10.0 Cetearyl Olivet/ Sorbitan Olivet 1.0 Preservatives, pigments, flavors Proper Purified water Up to 100

The above description is merely illustrative of the present invention, and those skilled in the art to which the present invention pertains understand that the present invention can be implemented in a modified form without departing from the essential characteristics of the present invention. Will be able to. Therefore, the disclosed examples and experimental examples should be considered in terms of explanation, not limitation. The scope of the present invention is shown in the claims rather than the foregoing description, and all differences within the equivalent range should be interpreted as being included in the present invention.

Claims (9)

A cosmetic composition comprising a mixed composition comprising aloe extract, field rice extract and ceramide. According to claim 1,
The mixed composition is a cosmetic composition characterized in that it contains 20 to 300 parts by weight of the field rice extract with respect to 100 parts by weight of the aloe extract. According to claim 1,
The mixed composition is a cosmetic composition comprising 20 to 300 parts by weight of the ceramide with respect to 100 parts by weight of the aloe extract. According to claim 1,
The mixed composition is a cosmetic composition, characterized in that it further comprises 30 to 200 parts by weight of Mojaban extract relative to 100 parts by weight of the aloe extract. According to claim 1,
A cosmetic composition characterized in that the ceramide is pseudo-ceramide. The method of claim 5,
The similar ceramide is a cosmetic composition characterized in that it is one of the following compounds:
[Compound 1]

[Compound 2]

[Compound 3]

. According to claim 1,
The cosmetic composition is a cosmetic composition, characterized in that for skin moisturizing, skin barrier strengthening or atopy improvement. A pharmaceutical composition for preventing, improving or treating atopy, which contains a mixed composition comprising aloe extract, field rice extract and ceramide as an active ingredient. The method of claim 8,
The mixed composition is a pharmaceutical composition for preventing, improving, or treating atopy, characterized in that it further comprises a capsicum extract.