KR101490762B1 - Cosmetic composition comprising the polysaccharide of Hibiscus esculentus as active ingredient - Google Patents

Abstract

The present invention relates to a process for the preparation of Lagerstroemia indica ) polysaccharide as an active ingredient, wherein the okra polysaccharide is characterized by containing 0.001 to 30.0% by weight based on the total weight of the cosmetic composition. According to the present invention, the okra polysaccharide extract has an excellent antioxidative effect, an inhibitory effect on MMP-1 production, and an effect of promoting collagen synthesis, and the cosmetic composition containing the same has a moisturizing effect, a skin barrier function recovery effect, great.

Description

A cosmetic composition comprising an okra polysaccharide as an active ingredient (Cosmetic composition comprising a polysaccharide of Hibiscus esculentus as active ingredient)

The present invention relates to a cosmetic composition containing an okra polysaccharide as an active ingredient.

The stratum corneum (SC), which is the outermost part of the skin, is directly exposed to the external environment and has the largest surface area in the human body. Therefore, it protects the external environment from harmful microorganisms, physical damage, It protects the body. In addition, it plays an important role in regulating body temperature and moisture content. The SC layer is a lipid mixture of ceramide, cholesterol, and fatty acids, which inhibits the penetration of foreign substances and prevents the amount of water evaporated from the surface of the skin, ie, Tranaepideramal Water Loss (TEWL) . In addition, natural keratin cells contain a high concentration of Natural Moisture Factor (NMF), which lowers the tension on the surface of the skin and has a repulsive force from the keratin to directly affect the moisturizing effect and inhibit drying of the skin. Clinically, dry skin is dry on the surface of the skin and is less elastic, and it is prone to keratin scaling. In some cases, itchy symptoms and skin cracking may occur.

 In general, the moisturizing agent is applied to the surface of the skin to improve the symptoms of diseases caused by the skin dryness such as moisture supply, maintenance and atopy to relieve and prevent various symptoms of dry skin. However, conventional moisturizers use a humectant which absorbs water and an occlusive moisturizer which prevents moisture evaporation, thereby increasing the moisture retention in the stratum corneum. The above-mentioned moisturizing agent is disadvantageous in that it is tacky when applied to the skin or difficult to maintain the stability of the emulsified formulation of the cosmetic.

 Recently, a moisturizing agent having excellent affinity has been widely used since it forms a moisture barrier film on the skin in the form of polysaccharide. Until now, hyaluronic acid and yugenpy (Korean Patent Publication No. 10-2009-0098083), shiitake mushroom (Korean Patent Laid-Open Publication No. 10-2012-0078327), ginseng (Korean Patent Laid-open No. 10-2001-0088981) , Tooth seed (Korean Patent Laid-Open No. 10-2010-0103351) and leaf mushroom (Korean Patent Laid-Open No. 10-2013-030013).

In human skin, various physical and chemical changes occur during the aging process. The cause of this phenomenon is divided into intrinsic aging and photo-aging. Free radicals can be caused by activation of ultraviolet rays, stress, disease state, environmental factors, wounds, and aging. When such state is deepened, the antioxidant defense network existing in the living body is destroyed, And aging.

More specifically, lipids, proteins, polysaccharides and nucleic acids, which are major components of the skin, are oxidized to destroy skin cells and tissues, resulting in skin aging.

In particular, the oxidation of proteins can cause collagen (collagen), hyaluronic acid, elastin, proteoglycan, fibronectin, and the like that are severely hyperinflammatory reactions and skin elasticity If this gets worse, DNA mutations can lead to mutations, cancer, and immune deficiency.

Therefore, it is necessary to protect the cell membrane by destroying the free radicals mediated by free radicals, ultraviolet rays, and inflammation that occur during the metabolism of the body, and to regenerate already damaged cells by vigorous metabolism The skin can quickly recover and maintain healthy skin.

Aging involves not only these free radicals, but also enzyme called matrix metalloproteinase (MMP), a collagenase. That is, synthesis and degradation of extracellular matrix such as collagen in vivo are appropriately controlled, but collagen synthesis is reduced as aging progresses, and expression of matrix metalloproteinase (MMP), an enzyme that degrades collagen, is promoted, And the wrinkles are formed. These degrading enzymes are also activated by ultraviolet irradiation.

Therefore, there is a demand for development of a substance capable of modulating MMP expression induced in the cell or inhibiting its activity. Until now, the raw materials used as cosmetic materials mostly inhibited only the enzyme activity. Therefore, we tried to control the amount and activity of MMP expression induced by intracellular natural aging and photoaging.

The inventors of the present invention have studied the applicability of various natural substances not yet known to cosmetics and found that the extract of Okra polysaccharide obtained from Hibiscus esculentus has a skin antioxidative effect, an inhibitory effect on MMP-1 production, and an effect of promoting collagen synthesis And found that it is very excellent and can expect efficacy as a cosmetic product. In addition, when such an okara polysaccharide is used as an active ingredient in a cosmetic composition, it is found that not only excellent feeling of use such as spreadability, moisturizing, stickiness and cushion feeling is excellent, but also moisturizing effect, recovery effect of skin barrier function and effect of improving skin wrinkle are excellent. .

In order to achieve the above object, the present invention provides a cosmetic composition comprising an okra ( Hibiscus esculentus or Abelmoschus esculentus ) polysaccharide as an active ingredient.

Preferably, the cosmetic composition is characterized by being for antioxidation.

Further, the cosmetic composition is characterized in that it is for moisturizing.

Further, the cosmetic composition is characterized in that it is for improving the skin barrier function.

Further, the cosmetic composition is characterized in that it is for improving skin wrinkles.

Further, the cosmetic composition is characterized in that it is for preventing skin aging.

Further, the cosmetic composition is characterized in that it is for use feeling improvement.

Preferably, the okra polysaccharide is contained in an amount of 0.001 to 30.0% by weight based on the total weight of the cosmetic composition.

Also, the okra polysaccharide is an extract obtained from various organs of okra, wherein the okra is an okra fruit, a leaf, a juvenile, a stem, a skin or a root.

The okra polysaccharide according to the present invention was excellent in antioxidative effect (Experimental Example 3), MMP-1 production inhibitory effect (Experimental Example 4) and collagen synthesis enhancing effect (Experimental Example 5) 6), skin barrier function recovery effect (Experimental Example 7), skin wrinkle improving effect (Experimental Example 8) and feeling feeling improving effect (Experimental Example 9).

In order to achieve the above object, the present invention provides a cosmetic composition comprising an okra polysaccharide as an active ingredient.

Okra ( Hibiscus esculentus or Abelmoschus esculentus ) is a plant belonging to the family Malvaceae which is cultivated in tropical and subtropical areas. It has a lot of fiber and it has a mucus, which is a mixture of pectin, galactam and araban, . It is also known to be rich in vitamin C and is good for fatigue recovery. In ancient times, it has been used as a poultice medicine to relieve the pain of leaf and less ripe fruit. Okra's polysaccharides have also been used in fields other than food (Ndjouenkeu, Goycoolea, Morris, & Akingbala, 1996; Tanaka et al., 2002; Woolfe, Chaplin, & Otchere, 1997). However, there have been no studies to use the polysaccharide of Okra as a moisturizing and skin aging composition for cosmetics.

In the present invention, the okra polysaccharide means an okra extract (hereinafter, an okra polysaccharide extract) containing at least one polysaccharide extracted from an okra (hereinafter, an okra polysaccharide) or a polysaccharide of an okra in a considerable amount. Further, the okra polysaccharide or the okra polysaccharide extract is obtained by extracting from various organs or parts (such as fruits, leaves, roots, stems, branches, branches, bark and seeds) of the okra, Mature, most preferably peeled from fruit, leaves, young branches, stems, branches, bark or roots, more preferably from fruit, leaves, young branches or roots, even more preferably from fruit or young branches Extracted from the resulting fruit.

The okra polysaccharide of the present invention can be extracted by a compression method or a commonly used solvent extraction method. That is, it can be extracted using a conventional solvent under the conditions of ordinary temperature and pressure, and may be filtered with a filter of a certain size at a constant pressure without using a solvent. (A) water, anhydrous or low-boiling alcohols having 1-4 carbon atoms (for example, methanol, ethanol, propanol and butanol), propylene glycol, 1,3-butylene glycol , At least one extraction solvent selected from the group consisting of glycerin, acetone, diethyl ether, ethyl acetate, butyl acetate, dichloromethane (methylene chloride), chloroform, hexane and mixtures thereof, Methylene chloride, chloroform or water, and most preferably 50% (v / v) methylene chloride.

On the other hand, it is apparent to those skilled in the art that the extract of Okra polysaccharide or polysaccharide of the present invention can be obtained not only by the above-mentioned extraction solvent but also by using other extraction solvent.

The okra polysaccharide or polysaccharide extract of the present invention can be prepared into a powder state by an additional process such as vacuum distillation and freeze drying or spray drying.

According to a more preferred embodiment of the present invention, the okra polysaccharide of the present invention is excellent in antioxidative effect, inhibitory effect on MMP-1 production, effect on enhancing collagen synthesis, moisturizing effect, restoring skin barrier function, And when the okra-derived polysaccharide of the present invention is prepared with a cosmetic composition, the skin condition improving effect as described above can be obtained.

According to the present invention, the above-mentioned okra polysaccharide is contained in an amount of 0.001-30.0 wt% with respect to the total weight of the cosmetic composition, and more preferably, the okra extract is contained in an amount of 0.01-30 wt% with respect to the total weight of the cosmetic composition do. When the content of the extract is less than 0.001% by weight, the skin is not improved.

The ingredients contained in the cosmetic composition of the present invention may contain, as an active ingredient, the ingredients commonly used in cosmetic compositions in addition to the above-mentioned effective ingredients, and may contain conventional ingredients such as antioxidants, stabilizers, solubilizers, vitamins, Supplements, and carriers.

The cosmetic composition of the present invention can be prepared into any of the formulations conventionally produced in the art and can be used in the form of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, , Oil, powdered foundation, emulsion foundation, wax foundation, pack, massage cream and spray, but is not limited thereto. More specifically, it can be manufactured in the form of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder.

When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

In the case where the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide ether Alkylamido betaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester.

When the cosmetic composition of the present invention is a soap, a surfactant-containing cleansing formulation or a surfactant-free cleansing formulation, it may be applied to the skin and then wiped off, washed or rinsed with water. The surfactant-containing cleansing formulation may be a cleansing foam, a cleansing water, a cleansing towel and a cleansing pack. The surfactant-free cleansing formulation may be a cleansing cream, , Cleansing lotion, cleansing water and cleansing gel, but is not limited thereto.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are only for describing the present invention in more detail and that the scope of the present invention is not limited by these embodiments in accordance with the gist of the present invention .

Production Example 1: Production of Okra polysaccharide extract

10.0 g of the mature okra fruit from which the peel was removed was added with 5 times of methylene chloride and purified water of the same amount, and the mixture was stirred for 30 minutes. The water layer was separated and concentrated to obtain about 1.03 g of an okara polysaccharide powder.

Production Example 2 Production of Okra polysaccharide extract

10.0 g of the mature okra fruit which had been removed from the peel was crushed finely, and then 5 times of purified water was added thereto. The mixture was centrifuged at 2,000 rpm, and the supernatant was fractionated and concentrated to obtain about 1.07 g of a polysaccharide powder.

Preparation Example 3: Preparation of Okra polysaccharide extract

10.0 g of the mature okra fruit without the rind was crushed, and the crushed okra fruit was wrapped with a 400 mesh filter, and a certain pressure was manually applied to obtain an okra polysaccharide, which was then concentrated to obtain about 0.94 g of a polysaccharide.

Manufacturing example  4: okra  Production of polysaccharide extract

10.0 g of the mature okra fruit from which the rind was removed was heated at 60 캜 at 60 캜 for about 2 to 3 minutes and then filtered to obtain a filtrate, which was then concentrated to obtain about 1.23 g.

Manufacturing example  5: okra  Production of polysaccharide extract

10.0 g of the mature okra fruit from which the rind was removed was mixed with 5 times of chloroform and the same amount of purified water was added. After stirring for 30 minutes, the water layer was separately fractionated and concentrated to obtain about 1.12 g of an okara polysaccharide powder.

Experimental Example 1: Measurement of sugar content of the okra polysaccharide

The sugar content of the extract of polysaccharide isolated from okra was measured by phenol - sulfuric acid method. 30 μl of 5% phenol solution and 0.2 ml of concentrated sulfuric acid were added to 20 μl of a solution in which the okra polysaccharide was dissolved in distilled water at a concentration of 0.1 mg / ml, and the mixture was reacted at 60 ° C. for 20 minutes and absorbance was measured at 490 nm. At this time, a standard capacity curve was prepared using glucose as a standard product. The sugar content of the okra polysaccharide was as shown in Table 1 below.

Experimental Example 2: Measurement of protein content of okra polysaccharide

The protein content of the polysaccharide isolated from the okra was measured using a Protein Assay Kit (Bio-Rad) manufactured based on the Bradford method. The standard dose curves were prepared using bovine serum albumin as a standard. The protein content of the okra polysaccharide was as shown in Table 1 below.

extract Sugar content (%) Protein content (%) Production Example 1 98.5 0.15 Production Example 2 99.5 0.11 Production Example 3 98.9 0.01 Production Example 4 99.4 0.05 Production Example 5 98.7 0.03

Experimental Example 3: Measurement of antioxidative effect by DPPH method

The antioxidative effect (free radical scavenging ratio) was measured by the DPPH method as follows to measure the antioxidative effect of the okra polysaccharide extracts obtained in Production Examples 1 to 5.

The DPPH method measures the antioxidative effect by reducing power using a free radical called DPPH (2,2-Di (4-tert-octylphenyl) -1-picrylhydrazyl) free radical. The degree of free radical scavenging (%) at a wavelength of 560 nm is measured by comparing the degree of decrease in absorbance of DPPH by the test substance (Okra extract) with the absorbance of the blank test solution.

As a reagent used, 61.88 mg of a 0.1 mM solution of 2,2-Di (4-tert-octylphenyl) -1-picrylhydrazyl free radical (Aldrich Chem. Co., MW = 618.76) was dissolved in methanol to make 100 ml.

As a measuring method

(1) Add 0.15 ml of the sample solution to 0.15 ml of 0.1 mM DPPH solution in a 96-well plate, stir rapidly, and start culturing at 25 ° C for 10 minutes.

② Measure the absorbance St at 560 nm thereafter.

(3) The blank of the sample solution is measured by the same procedure as that for measuring the absorbance St except that methanol is used instead of the 0.1 mM DPPH solution.

(4) The absorbance Bt is measured by operating in the same manner as in the measurement of the absorbance St except that distilled water is used instead of the sample solution.

(5) Blank of the blank test is carried out in the same manner as in the measurement of the absorbance St except that methanol is used instead of the 0.1 mM DPPH solution and distilled water is used instead of the sample solution, and the absorbance Bo is measured.

The results of the free radical scavenging ratio (%) were calculated according to Equation (1), and the results are shown in Table 2 below.

St: absorbance at 560 nm after free radical scavenging of the sample

Bt: absorbance at 560 nm after free radical scavenging of the blank test solution

So: the absorbance at 560 nm before the reaction in the absence of the free radical of the sample

Bo: absorbance at 560 nm before the reaction in the absence of the free radical of the blank test solution

  Name of sample Treatment concentration
(ppm) activity (%) Production Example 1 Production Example 2 Production Example 3 Production Example 4 Production Example 5 okra
extract 300 94.82 93.21 93.22 93.11 93.21 150 95.25 92.66 92.89 85.35 92.66 75 92.30 89.36 93.55 61.33 89.36 37.5 64.39 55.66 74.21 30.22 55.66 18.75 33.38 33.77 60.21 18.74 33.77 Vit-C 300ppm 78.98

As can be seen from the results in Table 3, in the free radical scavenging experiment by DPPH, it was confirmed that the extract of Okra and the okra polysaccharide according to the present invention showed an increase in the antioxidative effect in a concentration dependent manner. The free radical scavenging rate (Vit-C, as a comparative example), which is a substance known to have an antioxidant effect.

Experimental Example 4. Inhibitory Effect on MMP-1 Production

The following experiments were carried out in order to measure the effect of the extracts of the okra polysaccharides obtained in Production Examples 1 to 5 on the production of collagenase MMP-1.

Fibroblasts (Korean Cell Line Bank, Korea), human normal skin cells, were inoculated into 48-well microplates (Nunc, Denmark) at a concentration of 1 10 ⁶ cells per well and cultured in DMEM medium (Sigma, For 24 hours, and then cultured in serum-free DMEM medium supplemented with the okra polysaccharide extracts of Preparation Examples 1 and 2 and in a serum-free DMEM medium containing no okra polysaccharide extract for 48 hours as a control group.

After culturing, the supernatant of each well was collected and the amount (ng / ml) of the newly synthesized MMP-1 was measured using an MMP-1 assay kit (Amersham, USA) The inhibition rate (%) was calculated and the results are shown in Table 3.

  Name of sample Treatment concentration
(ppm) activity (%) Production Example 1 Production Example 2 Production Example 3 Production Example 4 Production Example 5 okra
extract 300 97.25 89.37 90.69 92.94 90.31 150 85.33 81.24 84.32 89.54 84.58 75 75.23 64.38 62.98 48.44 67.44 37.5 64.65 53.32 47.25 35.13 45.34 18.75 55.17 25.23 23.45 25.75 35.22 TGF-beta 200 nM 75.3

As shown in the results of Table 3, it was confirmed that the Okra polysaccharide extract of the present invention inhibits MMP-1 production (inhibits MMP-1 activity) in a concentration-dependent manner. It was confirmed that MMP-1 production inhibitory effect was superior to the effect of TGF-beta (200 nM, as a comparative example), which is known to inhibit MMP-1 production.

Experimental Example 5. Effect of Okra extract on collagen synthesis

Human normal epithelial cells, fibroblasts, were inoculated on a 48-well microplate at a density of 1 × 10 ⁶ cells per well, and cultured in DMEM medium for 24 hours. Serum-free DMEM medium supplemented with the okra polysaccharide extracts prepared in Preparation Examples 1 to 21 and a control group were further cultured for 48 hours in serum-free DMEM medium containing no oak polysaccharide extract. After the incubation, the supernatant of each well was collected, and the amount of procollagen type I C-peptide (PICP) was measured using a collagen kit (Takara, Japan) and converted into ng / Respectively. The percent increase in collagen biosynthesis was calculated according to the following equation (3), and the results are shown in Table 4.

  Name of sample Treatment concentration
(ppm) activity (%) Production Example 1 Production Example 2 Production Example 3 Production Example 4 Production Example 5 Okra extract 18.75 23.1 11.24 13.1 12.1 11.24 37.5 43.3 31.12 33.89 33.4 21.12 75 51.9 45.87 60.59 51.5 35.87 150 84.1 58.50 85.64 82.2 38.50 300 133.65 60.35 109.98 113.5 41.35 TGF-beta 200 nM 175.23

As can be seen from Table 4, it was confirmed that the collagen biosynthesis rate of the okra polysaccharide extract prepared according to Production Examples 1 to 5 of the present invention increased in a concentration-dependent manner.

Formulation Examples 1 to 3: Preparation of cosmetic composition containing Okra extract

The cosmetic comprising the okra extract was prepared the cosmetic composition (formulation example 1, 2, 3) comprising a okra polysaccharide of Production Example 1, Production Example 3 and Production Example 4 extract, respectively, in place of okra polysaccharide extract for comparison of efficacy A cosmetic composition (Comparative Formulation Example 1) containing glycerin and 1,3-butylene glycol as a moisturizer, a cosmetic composition (Comparative Formulation Example 2) containing glycerin and sorbitol as a moisturizing agent, a cosmetic composition (Comparative Formulation Example 3) are shown in Table 5 below.

division ingredient Formulation Example 1 Formulation Example 2 Formulation Example 3 Comparative Formulation Example 1 Comparative Formulation Example 2 Comparative Formulation Example 3 A Okra extract
(Production Example 1) 5.0 - - - - Okra extract
(Production Example 3) - 5.0 - - - Okra extract
(Production Example 4) 5.0 glycerin 5.0 5.0 1,3-butylene glycol - - 5.0 - - Sorbitol - - - 5.0 - EDTA-2Na 0.02 0.02 0.02 0.02 0.02 0.02 Purified water to 100 to 100 to 100 to 100 to 100 to 100 B Cetostearyl alcohol 2.0 2.0 2.0 2.0 2.0 2.0 Glyceryl stearate 1.5 1.5 1.5 1.5 1.5 1.5 Microcrystalline 0.7 0.7 0.7 0.7 0.7 0.7 Squirrel 5.0 5.0 5.0 5.0 5.0 5.0 Liquid paraffin 3.0 3.0 3.0 3.0 3.0 3.0 Trioctanoin 5.0 5.0 5.0 5.0 5.0 5.0 Polysorbate 1.2 1.2 1.2 1.2 1.2 1.2 Sorbitan stearate 0.5 0.5 0.5 0.5 0.5 0.5 Tocopheryl acetate 0.2 0.2 0.2 0.2 0.2 0.2 Cyclomethicone 3.0 3.0 3.0 3.0 3.0 3.0 BHT 0.05 0.05 0.05 0.05 0.05 0.05 C Incense, preservative Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount

Experimental Example 6: Moisturizing Effect of Okra Polysaccharide Extract

The clinical moisturizing effects of the cosmetic compositions of Formulation Examples 1, 2 and 3 were measured as follows. A, B, and C groups were divided into five groups (A, B, C, D, and E) by a randomly selected group of 25 healthy adult men and women who felt skin dry through the questionnaire. (Comparative Formulation Example 1) containing glycerin and 1,3-butylene glycol as moisturizing agents instead of an okra polysaccharide extract, and a cosmetic composition comprising glycerin and sorbitol as a moisturizing agent (comparison Formulation Example 2) were each applied to the face for 8 weeks twice a day. The moisturizing effect was evaluated by Corneometer CM 820 (Corage + Khazaka, Germany) for every 2 weeks and after the start of the test. The experimental results are shown in Table 6 below.

Skin moisturizing effect of Okra polysaccharide extract division Before use After 2 weeks of use After 4 weeks of use Formulation Example 1 23.5 38.6 45.7 Formulation Example 3 23.2 36.4 39.6 Formulation Example 4 23.2 37.8 45.4 Comparative Formulation Example 1 24.5 35.9 40.8 Comparative Formulation Example 2 22.8 36.5 39.9 Comparative Formulation Example 3 22.8 22.8 22.8

As can be seen from the results of Table 6 above, compared with cosmetics containing other moisturizers (Comparative Formulation Examples 1, 2, and 3), the cosmetic compositions containing the okra polysaccharide extract of the present invention (Formulation Examples 1, 2 and 3) And it was confirmed that the moisturizing effect was excellent.

Experimental Example 7: Recovery effect of skin barrier function

In order to examine the skin barrier function recovery effect of the cosmetic composition containing the okra polysaccharide extract of the present invention, the following experiment was conducted. Experiments were carried out by using a taping stripping method to damage the skin barrier and measuring the skin water loss (TEWL, transdermal water loss) to 4.0 g / m 2 / h measured by servomed (Sweden) evaporimeter EP 1 , Formulation Examples 1 to 3 and Comparative Formulation Examples 1 to 3 each containing the ocher polysaccharide extract of the present invention prepared in Preparation Examples 1, 3 and 4 were applied to an area of 5 cm 2, , 2 hours, 4 hours, and 8 hours after the administration, and evaluated for the extent to which the barrier function was restored. At this time, a known lipid mixture (ceramide: 2: 1: 1 mixture of cholesterol: fatty acid) was used as a positive control. The results are shown in Table 7 below with reference to the time-dependent changes in skin moisture loss at the initial damaged barrier (100%).

sample 0 hours 1 hours 2 hours 4 hours 8 hours Formulation Example 1 85 61 39 23 23 Formulation Example 2 85 63 35 21 21 Formulation Example 3 85 66 36 23 22 Comparative Formulation Example 1 85 70 60 50 41 Comparative Formulation Example 2 85 75 63 52 43 Comparative Formulation Example 3 85 75 67 55 25

As can be seen from the results of Table 7, it was confirmed that Formulation Examples 1 to 3, which are the cosmetic compositions containing the okra polysaccharide extract of the present invention, have a superior skin barrier function recovery effect as compared with Comparative Formulations 1 to 3.

Experimental example 8. Effect of improving wrinkles of skin

Clinical trials of the skin wrinkle improving effect of the cosmetic composition containing the okra polysaccharide extract of the present invention were conducted. Clinical trials were evaluated through actual use tests of each cosmetic product.

That is, the clinical test was conducted in the same manner as in Example 1 except that the cosmetic composition of Comparative Formulation Example 1 containing formulation of Okra polysaccharide of Preparation Example 1 and glycerin and 1,3-butylene glycol as a moisturizer instead of the okra polysaccharide extract And the effect of improving skin wrinkles due to the use of cosmetics was confirmed and compared.

Twenty patients (20 to 35 years old) were applied to Formulation Example 2 on the right side of the face and Comparative Formulation Example 1 on the left side of the face for 2 consecutive months, respectively.

Sixty female subjects were divided into 20 experimental groups, each of which was treated with a cosmetic composition (Comparative Formulation Example 1) containing glycerin and 1,3-butylene glycol as a moisturizing agent instead of an okra polysaccharide extract, and 3 groups of adenosine 3% (v / v) (Comparative Formulation Example 4), and the test group of Formulation Example 2 containing an okra polysaccharide extract. The wrinkle improvement effect after 6 weeks was visually evaluated using both sides of the face And the degree of wrinkle improvement was confirmed.

The wrinkle improvement effect of each subject before and after 3 months of use was evaluated by a skilled physician through visual observation and instrument measurement (cutometer SEM 575, C + K Electronic Co., Germany) The results of the base wrinkle improvement effect are shown in Table 8 below

Wrinkle improvement effect (visual evaluation and device evaluation) Wrinkle improvement effect Effective rate (%) Great slightly none Formulation Example 1
(Okra polysaccharide extract of Preparation Example 1) 24 4 2 85.0 Comparative Formulation Example 1
(Moisturizer) 3 4 23 25.0 Comparative Formulation Example 4
(Adenosine) 17  7 7  80.0

As can be seen from the results of Table 8, the cosmetic composition of Formulation Example 1 containing the okra polysaccharide extract of the present invention showed a wrinkle-improving effect about 3.4 times higher than Comparative Formulation Example 1 containing moisturizing agent.

In addition, similar results were obtained with the cosmetic preparation of Comparative Example 4 containing adenosine, which is known to have a wrinkle-reducing effect instead of the okra polysaccharide extract, and it was found that the excellent wrinkle-reducing effect of the okra polysaccharide extract was exhibited. In addition, skin irritation could not be observed in most of the subjects who applied the cosmetic composition containing the okra polysaccharide extract of the present invention to the skin.

Experimental Example 9. Evaluation of feeling improvement effect

Evaluation of feeling improvement effect was carried out as follows. Twenty women between the ages of 30 and 40 were randomly divided into two groups. After washing the cream of Formulation Example 1 and the cream of Comparative Formulation 1 twice daily morning and evening, an appropriate amount of the cosmetic was continuously and centered around the eyes. The feelings of each subject were evaluated through a user questionnaire. The experimental results are shown in Table 9 below. The effect of improving the sensation of use of the cosmetic of the present invention was evaluated through practical use tests. After using the nutritional creams of Formulation Example 1 and Comparative Formulation Example 1 on the skin, the feeling of use improvement effect was evaluated.

Effect of feeling improvement (questionnaire evaluation) sample Brittle Stickiness Cushion feeling Yu Chen Adhesiveness Formulation Example 1 9.6 1.8 9.5 9.6 9.9 Comparative Formulation Example 1 5.5 5.2 6.4 6.7 7.1

The results were as shown in Table 10. According to the results shown in Table 10 above, the cream of the present invention containing an okra polysaccharide extract is comparable to the moisturizers and Comparative Formulation Examples 1 and 2 used as 1,3-butylene glycol and glycerin, As a result, it was found that the feeling of feeling of using cosmetics was excellent, and the stickiness was very small. Therefore, it has been confirmed that the extract of Okra polysaccharide of the present invention has a very excellent effect as an agent for improving sensation of use in cosmetics.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the same is by way of illustration and example only and is not to be construed as limiting the scope of the present invention. It is therefore intended that the scope of the invention be defined by the claims appended hereto and their equivalents.

Claims (7)

delete An antioxidant cosmetic composition containing an okra polysaccharide extracted from fruit of a mature Hibiscus esculentus which has been removed from the skin, as an active ingredient. Wherein the composition is for improving skin barrier containing an okra polysaccharide extracted from a mature Hibiscus esculentus fruit with the skin removed as an active ingredient. delete A cosmetic composition for improving the feeling of use comprising an okra polysaccharide extracted from a fruit of a mature Okra ( Hibiscus esculentus ) from which a skin is removed as an active ingredient. The cosmetic composition according to any one of claims 2, 3, and 5, wherein the okra polysaccharide is contained in an amount of 0.001 to 30.0% by weight based on the total weight of the cosmetic composition. The method of any one of claims 2, 3 and 5, wherein the okra polysaccharide is selected from the group consisting of water, an anhydrous or a lower alcohol having 1-4 carbon atoms, propylene glycol, butylene glycol, glycerin, acetone, ethyl acetate, chloroform , Butyl acetate, diethyl ether, dichloromethane, hexane, and mixtures thereof.