KR20140071713A - Cosmetic composition comprising the extract of Metaplexis japonica as active ingredient - Google Patents

Abstract

The present invention relates to a cosmetic composition containing a Metaplexis japonica extract as an active ingredient, which is characterized by containing 0.001-30.0 wt% of the Metaplexis japonica extract for a total weight of a cosmetic composition as an active ingredient. According to the present invention, the Metaplexis japonica extract has an anti-oxidation effect, an MMP-1 generation preventing effect, a collagen biosynthesis effect, an antiseptic effect, and a moisturizing effect, so as to provide various kinds of functional cosmetic materials.

Description

[0001] The present invention relates to a cosmetic composition comprising an extract of Panax notoginseng as an active ingredient,

The present invention relates to a cosmetic composition comprising an extract of Bacillus subtilis as an active ingredient.

A variety of physical and chemical changes occur in human skin during the aging process. The cause of aging is divided into intrinsic aging and photo-aging. Free radicals can be caused by activation of ultraviolet rays, stress, disease state, environmental factors, wounds, and aging. When such state is deepened, the antioxidant defense network existing in the living body is destroyed, And aging.

That is, if skin, cells, proteins, polysaccharides and nucleic acids are oxidized and the skin cells and tissues are destroyed, skin aging eventually occurs.

In particular, the oxidation of proteins can cause collagen (collagen), hyaluronic acid, elastin, proteoglycan, fibronectin, and the like that are severely hyperinflammatory reactions and skin elasticity If this gets worse, DNA mutations can lead to mutations, cancer, and immune deficiency.

Therefore, it is necessary to protect the cell membrane by destroying the free radicals mediated by free radicals, ultraviolet rays, and inflammation that occur during the metabolism of the body, and to regenerate already damaged cells by vigorous metabolism The skin can quickly recover and maintain healthy skin.

Aging involves not only these free radicals, but also enzyme called matrix metalloproteinase (MMP), a collagenase. That is, synthesis and degradation of extracellular matrix such as collagen in vivo are appropriately controlled, but collagen synthesis is reduced as aging progresses, and expression of matrix metalloproteinase (MMP), an enzyme that degrades collagen, is promoted, And the wrinkles are formed. These degrading enzymes are also activated by ultraviolet irradiation.

Therefore, there is a demand for development of a substance capable of modulating MMP expression induced in the cell or inhibiting its activity. Until now, the raw materials used as cosmetic materials mostly inhibited only the enzyme activity. Therefore, the present inventors tried to control the expression amount and activity of MMP induced by natural aging and photoaging using natural extracts.

Korean Patent Laid-Open No. 2008-0090806 discloses a cosmetic composition containing various natural products and having skin-improving effect.

Recently, many cosmetic products using natural products have been developed to reduce skin irritation caused by various chemical substances and the like. Natural materials are not only less harmful to the skin, but also have increased their value as a raw material for cosmetics due to the recent popularity of cosmetics using natural materials.

The inventors of the present invention have studied the applicability of various natural products to cosmetics. As a result, the present inventors have found that an antioxidative effect, an inhibitory effect on MMP-1 production, a collagen As a result of measuring the synthetic enhancing effect, the moisturizing effect and the preservative effect, it was found that the efficacy as a cosmetic product can be expected because of its excellent effect.

It is an object of the present invention to provide a cosmetic composition containing an antioxidant extract, a skin aging-preventing effect, a skin wrinkle-improving effect, a preservative effect, a moisturizing effect or a skin wrinkle-improving effect.

It is also an object of the present invention to provide a cosmetic composition that exhibits an efficacy by preparing a supercritical extract and an ultrasound extract and purifying the extract to maximize the efficacy of the extract.

Another object of the present invention is to provide a cosmetic method using the cosmetic composition containing the saccharide extract.

Accordingly, the object of the present invention as described above is achieved by a cosmetic composition comprising an extract of bamboo shoots as an active ingredient.

Preferably, the cosmetic composition is characterized by being for antioxidation.

Preferably, the cosmetic composition is characterized in that it is for preventing skin aging.

Preferably, the cosmetic composition is characterized in that it is for improving skin wrinkles.

Preferably, the cosmetic composition has a preservative effect.

Preferably, the cosmetic composition is characterized in that it is for moisturizing.

Further, it is preferable that the above-described bergamot extract is contained in an amount of 0.0001 to 30.0% by weight based on the total weight of the cosmetic composition.

In addition, preferably, the puerariae extract is selected from the group consisting of water, an anhydrous or a lower alcohol having 1-4 carbon atoms, propylene glycol, butylene glycol, glycerin, acetone, ethyl acetate, chloroform, butyl acetate, diethyl ether, dichloromethane, And a mixture thereof, or a solvent extraction method or an ultrasonic extraction method using the extraction solvent.

Preferably, the cosmetic composition is to provide a cosmetic composition that exhibits skin antioxidant effect, MMP-1 production inhibiting effect, collagen synthesis promoting effect, moisturizing effect, and skin wrinkle improving effect.

Preferably, the cosmetic composition is a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, cleansing oil, powder foundation, emulsion foundation, wax foundation, pack, massage cream and spray Lt; RTI ID = 0.0 > of: < / RTI >

The extract of Bacillus japonica extract according to the present invention was found to have antioxidant effect on skin (Experimental Example 1), inhibitory effect on MMP-1 production (Experimental Example 2), effect on enhancing collagen synthesis (Experimental Example 3), moisturizing effect (Experimental Example 4) Example 6) and a wrinkle-improving effect (Experimental Example 7).

Further, according to the present invention, the antioxidative effect, the inhibitory effect on the production of MMP-1 and the enhancing effect on collagen synthesis are further enhanced through ultrasound and supercritical extraction in order to maximize the efficacy of the extract.

Metaplexis japonica , which is used as an active ingredient in the cosmetic composition of the present invention, is a perennial herbaceous perennial native to Korea. The growing environment grows in a fertile and sunny soil. The key grows to about 3m in length. Leaves are 5 ~ 10cm long and 3 ~ 6cm wide, with no hairs, pointed end, and white on back. Flowers are 2 ~ 5cm long and have flower peduncle and light purple color. The fruit runs from October to November and is shaped like a horn with a length of 10cm. There are many projections on the front side, and the seed is flat with a length of 0.6 ~ 0.8cm. However, there is nothing known about the efficacy of the pupa extract.

In the present invention, the bamboo shoot extract means a bamboo shoot extracted from various organs or parts (e.g., leaves, roots, stems, branches, tall branches, bark and seeds) of the bamboo shoots, and preferably, leaves, Extracts from leaves, branches, roots or roots, more preferably extracts from leaves, leaves or roots, and most preferably extracts from leaves and leaves.

In addition, the extract of the present invention can be produced by using a conventional solvent according to a conventional method known in the art, that is, under the conditions of ordinary temperature and pressure. Preferably, (a) water, Propanol and butanol), propylene glycol, 1,3-butylene glycol, glycerin, acetone, diethyl ether, ethyl acetate, butyl acetate, dichloromethane (such as methanol, ethanol, (B) solvent extraction using a solvent selected from the group consisting of chloroform, hexane and mixtures thereof; (b) supercritical extraction with carbon dioxide and supercritical extraction with high temperature; or (c) extraction with ultrasonic extraction.

In the present invention, a solvent extraction method using an extraction solvent is preferably used. The extract is preferably prepared by extracting various extracting solvents, for example, water, an anhydrous or a lower alcohol having 1-4 carbon atoms (for example, methanol, ethanol, Butanol), propylene glycol, 1,3-butylene glycol, glycerin, acetone, diethyl ether, ethyl acetate, butyl acetate, dichloromethane, chloroform, hexane, , Preferably obtained using ethanol, 70% (v / v) ethanol or water, and most preferably 70% (v / v) ethanol.

On the other hand, it is obvious to those skilled in the art that the extract of the present invention can be obtained not only by the above-mentioned extraction solvent but also by other extraction solvent, which exhibits substantially the same effect.

In addition, the bamboo powder extract of the present invention includes not only the extract obtained by the above-described extraction solvent, but also the extract obtained through ordinary purification and fermentation processes. For example, decompression by carbon dioxide, extraction by supercritical extraction with high temperature, extraction by extraction using ultrasound, separation by ultrafiltration membrane with constant molecular weight cutoff value, various chromatography (size, charge, hydrophobic or affinity And the active fraction obtained through various purification and extraction methods, such as an extract made by fermentation products using natural or various microorganisms, are also included in the extract of the present invention.

Further, the present invention provides a cosmetic composition characterized in that the above extract is obtained by liquid-freezing obtained by cold-pressing and heating filtration at room temperature, and further by concentrating or lyophilizing the solvent under reduced pressure.

The supercritical fluid extraction refers to supercritical fluid extraction. Generally, the supercritical fluid is extracted from the liquid and the liquid when the gas reaches the critical point at high temperature and high pressure. (J. Chromatogr. A. 1998; 479: 200-205). In addition, it has been reported that the supercritical fluid has a polarity similar to that of a nonpolar solvent.

Carbon dioxide is a supercritical fluid with both liquid and gaseous nature, with the operation of supercritical fluid equipment reaching its critical pressure and temperature, resulting in increased solubility in fat-soluble solutes. When supercritical carbon dioxide passes through an extraction vessel containing a certain amount of sample, the lipophilic substance contained in the sample is extracted into supercritical carbon dioxide.

When the supernatant carbon dioxide containing a small amount of cosolvent is passed through the sample remaining in the extraction vessel after extracting the lipid-soluble substance, components that were not extracted by pure supercritical carbon dioxide can be extracted.

The supercritical fluid used in the supercritical extraction method of the present invention can effectively extract an active ingredient by using a mixed fluid in which a cosolvent is further mixed with supercritical carbon dioxide or carbon dioxide.

These co-solvents may be used alone or in admixture of two or more selected from the group consisting of chloroform, ethanol, methanol, water, ethyl acetate, hexane and diethyl ether.

Most of the extracted samples contain carbon dioxide. Since the carbon dioxide is volatilized into air at room temperature, the extract obtained by the above method can be used as a cosmetic composition, and the cosolvent can be removed by a reduced pressure evaporator.

In addition, the ultrasonic extraction method is an extraction method using energy generated by ultrasonic vibration. Ultrasonic waves can destroy an insoluble solvent contained in a sample in a water-soluble solvent. Due to the high local temperature generated at this time, Since the kinetic energy of the particles is increased, sufficient energy required for the reaction is obtained. By inducing the high pressure by the shock effect of the ultrasonic energy, the mixing efficiency of the substance contained in the sample and the solvent is increased, thereby increasing the extraction efficiency.

As the extraction solvent which can be used for the ultrasonic extraction method, one or a mixture of two or more selected from the group consisting of chloroform, ethanol, methanol, water, ethyl acetate, hexane and diethyl ether can be used. The extracted sample is recovered by vacuum filtration, and the filtrate is recovered, and the extract is removed by a vacuum evaporator and freeze-dried to obtain an extract.

The present invention also provides a fermented extract of Bacillus subtilis, which comprises a fermented extract. The flour is finely crushed to a size of about 100 to 500 mesh, then 1 to 50 g / L of a conventional microorganism culture solution is added, and microorganisms such as yeast strains or lactic acid bacteria are added in an amount of 10,000 to 100,000 cfu / L. The culture temperature is 30 to 37 ° C. The pH is 5 to 7 and aerobic or anaerobic conditions are maintained for about 5 to 10 days. Which can be obtained through aging and filtration.

According to the present invention, the pufferfish extract is contained in an amount of 0.001 to 30.0 wt% based on the total weight of the cosmetic composition, and more preferably, the pufferfish extract is contained in an amount of 0.01 to 10 wt% with respect to the total weight of the cosmetic composition do. When the content of the extract is less than 0.001% by weight, the effect of improving the skin is not exhibited. When the content of the extract is more than 30.0% by weight, the degree of improvement of the skin against the increase of the content is insignificant, It is not even.

Therefore, the cosmetic composition of the present invention including the bamboo powder extract having various functions of the present invention has excellent antioxidant effect, skin aging prevention effect, collagen synthesis promotion effect, MMP-1 production inhibitory effect, skin wrinkle improvement effect, moisturizing effect and preservative effect .

The ingredients contained in the cosmetic composition of the present invention may contain, as an active ingredient, the ingredients commonly used in cosmetic compositions in addition to the above-mentioned effective ingredients, and may contain conventional ingredients such as antioxidants, stabilizers, solubilizers, vitamins, Supplements, and carriers.

The cosmetic composition of the present invention can be prepared into any of the formulations conventionally produced in the art and can be used in the form of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, , Oil, powdered foundation, emulsion foundation, wax foundation, pack, massage cream and spray, but is not limited thereto. More specifically, it can be manufactured in the form of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder.

When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

In the case where the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

In the case where the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. Especially, in the case of a spray, a mixture of chlorofluorohydrocarbons, propane / Propane or dimethyl ether.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide ether Alkylamido betaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester.

When the cosmetic composition of the present invention is a soap, a surfactant-containing cleansing formulation or a surfactant-free cleansing formulation, it may be applied to the skin and then wiped off, washed or rinsed with water. The surfactant-containing cleansing formulation may be a cleansing foam, a cleansing water, a cleansing towel and a cleansing pack. The surfactant-free cleansing formulation may be a cleansing cream, , Cleansing lotion, cleansing water and cleansing gel, but is not limited thereto.

On the other hand, the cosmetic process of the present invention refers to all cosmetic processes using the cosmetic composition of the present invention. That is, all methods known in the art using a cosmetic composition belong to the cosmetic method of the present invention.

The cosmetic composition of the present invention may be used alone or in combination, or may be used in combination with other cosmetic compositions other than the present invention. The cosmetic composition according to the present invention may be used according to a conventional method of use, and may be used in a number of times depending on the skin condition or taste of the user.

By carrying out the cosmetic method using the cosmetic composition comprising the bamboo powder extract of the present invention, it is possible to obtain a skin antioxidant effect, an effect of inhibiting MMP-1 production, an effect of enhancing collagen synthesis, a moisturizing effect, a preservative effect and a skin wrinkle improving effect.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are only for describing the present invention in more detail and that the scope of the present invention is not limited by these embodiments in accordance with the gist of the present invention .

Manufacturing example  1: Manufacture of bamboo shoots extract

100 g of the shredded pearl liquor was refluxed three times for five hours in a 70% (v / v) aqueous ethanol solution of 5 times, cooled with ice, and filtered through a Whatman # 2 filter paper. The filtered extract was concentrated under reduced pressure and freeze-dried at 50 DEG C or lower.

Manufacturing example  2 to 4. Pusan Supercritical  Manufacture of fluid extracts and ultrasonic extracts

100 g of finely chopped pearl liquor was extracted with a supercritical extraction method (extracting with olive oil as a co-solvent in a supercritical state under a pressure of about 300 atm at a temperature of about 60 DEG C) to obtain a supercritical extract Example 2). 100 g of finely chopped pearl bean sprout was extracted with ultrasonic waves (at a temperature of 30 캜 for about 2 hours at a strength of 25 KHz using a supernatant ultrasonic instrument) to obtain an extract (Production Examples 3 and 4). In the case of Production Example 3, ethanol was used as a cosolvent, and in Production Example 4, purified water was used as a cosolvent.

Experimental Example  One: DPPH Antioxidant effect measurement

In Experimental Example 1, the antioxidative effect (free radical scavenging ratio) was measured using the DPPH method in order to measure the antioxidative effect of the brewer's drink extracts obtained in Production Examples 1 to 4.

The DPPH method measures the antioxidative effect by reducing power using a free radical called DPPH (2,2-Di (4-tert-octylphenyl) -1-picrylhydrazyl) free radical. The degree of free radical scavenging (%) at a wavelength of 560 nm is measured by comparing the degree of reduction of the absorbance with the absorbance of the blank test solution by reduction of DPPH by the test substance (baculovirus extract).

As a reagent used, 61.88 mg of a 0.1 mM solution of 2,2-Di (4-tert-octylphenyl) -1-picrylhydrazyl free radical (Aldrich Chem. Co., MW = 618.76) was dissolved in methanol to make 100 ml.

As a measuring method

(1) Add 0.15 ml of the sample solution to 0.15 ml of 0.1 mM DPPH solution in a 96-well plate, stir rapidly, and start culturing at 25 ° C for 10 minutes.

② Measure the absorbance St at 560 nm thereafter.

(3) The blank of the sample solution is measured by the same procedure as that for measuring the absorbance St except that methanol is used instead of the 0.1 mM DPPH solution.

(4) The absorbance Bt is measured by operating in the same manner as in the measurement of the absorbance St except that distilled water is used instead of the sample solution.

(5) Blank of the blank test is carried out in the same manner as in the measurement of the absorbance St except that methanol is used instead of the 0.1 mM DPPH solution and distilled water is used instead of the sample solution, and the absorbance Bo is measured.

The results of the free radical scavenging ratio (%) were calculated by the equation (1), and the results are shown in Table 1 below.

St: absorbance at 560 nm after free radical scavenging of the sample

Bt: absorbance at 560 nm after free radical scavenging of the blank test solution

So: the absorbance at 560 nm before the reaction in the absence of the free radical of the sample

Bo: absorbance at 560 nm before the reaction in the absence of the free radical of the blank test solution

Table 1 shows that the free radical scavenging activity of DPPH showed that the antioxidant activity of the anthelmintic extract of the present invention was increased in a dose dependent manner, It has been confirmed that the antioxidant effect of vitamin C, which is a known substance, is superior to that of antioxidant effect.

Experimental Example  2. Pumpkin extract MMP -1 production inhibitory effect

In Experimental Example 2, the effect of inhibiting the production of MMP-1, a collagenase, produced by the strains obtained in Preparation Examples 1 to 4 was measured.

Human normal skin cells were inoculated into a 48-well microplate (Nunc, Denmark) at a concentration of 1 × 10 ⁶ cells per well and cultured in DMEM medium (Sigma, USA) and 37 ° C For 24 hours. Then, the cells were further cultured in serum-free DMEM medium supplemented with the baculovirus extract of Preparation Examples 1 and 2 and in serum-free DMEM medium containing no bacilli extract for 48 hours.

After culturing, the supernatant of each well was collected and the amount (ng / ml) of the newly synthesized MMP-1 was measured using an MMP-1 assay kit (Amersham, USA) The inhibition rate (%) was calculated and the results are shown in Table 3.

As shown in Table 2, it was confirmed that the extract of the present invention inhibits MMP-1 production (inhibits MMP-1 activity) in a concentration-dependent manner. In particular, it was confirmed that the bamboo powder extract prepared by Preparation Example 1 was more effective than the other preparation examples. Even considering the difference between the concentration of TGF-beta (200 nM), which is known to have an inhibitory effect on MMP-1 production, and the concentration (300 ppm) of the brewer's liquor extract of the present invention, Showed that MMP-1 inhibitory effect of the present invention was inhibited by MMP-1.

Experimental Example  3. Enhancement of Collagen Synthesis Effect of Pakjuri Extract

Human normal epithelial cells, fibroblasts, were inoculated into 48-well microplates at a density of 1 x 10 ⁶ cells per well and cultured in DMEM medium for 24 hours. The cells were further cultured in serum-free DMEM medium supplemented with the bacilli extract prepared in Preparation Examples 1 to 4 and in serum-free DMEM medium containing no bacilli extract for 48 hours as a control group. After the incubation, the supernatant of each well was collected, and the amount of procollagen type I C-peptide (PICP) was measured using a collagen kit (Takara, Japan) and converted into ng / Respectively. Collagen biosynthesis increase rate (%) was calculated according to the following equation (3), and the results are shown in Table 3.

As shown in Table 3, it was confirmed that the collagen biosynthesis rate of the purified bovine Phragmites extract prepared by Production Examples 1 to 4 of the present invention increased in a concentration-dependent manner. In particular, it was confirmed that the extract prepared according to Preparation Example 1 had a better effect than the extracts of other preparations.

Formulation Example  1: Containing the extract of Bacillus thuringiensis Cosmetics  Preparation of composition

Cosmetic preparations containing Formosan extracts (Preparation Examples 1, 2 and 3) each containing the extracts of Formosan peel extracts of Preparation Example 1, Preparation Example 3 and Preparation Example 4 were prepared, and as a moisturizer (Comparative Formulation Example 1) containing glycerin and 1,3-butylene glycol, a cosmetic composition containing glycerin and sorbitol as a moisturizing agent (Comparative Formulation Example 2), a cosmetic composition containing no extract or moisturizer Formulation Example 3) is shown in Table 4 below.

division ingredient Formulation Example 1 Formulation Example 2 Formulation Example 3 Comparative Formulation Example 1 Comparative Formulation Example 2 Comparative Formulation Example 3 A






Pumpkin extract
(Production Example 1) 5.0 - - - - - Pumpkin extract
(Production Example 3) - 5.0 - - - - Pumpkin extract
(Production Example 4) - - 5.0 - - - glycerin - - - 5.0 5.0 - 1,3-butylene glycol - - - 5.0 - - Sorbitol - - - - 5.0 - EDTA-2Na 0.02 0.02 0.02 0.02 0.02 0.02 Purified water to 100 to 100 to 100 to 100 to 100 to 100 B









Cetostearyl alcohol 2.0 2.0 2.0 2.0 2.0 2.0 Glyceryl stearate 1.5 1.5 1.5 1.5 1.5 1.5 Microcrystalline 0.7 0.7 0.7 0.7 0.7 0.7 Squirrel 5.0 5.0 5.0 5.0 5.0 5.0 Liquid paraffin 3.0 3.0 3.0 3.0 3.0 3.0 Trioctanoin 5.0 5.0 5.0 5.0 5.0 5.0 Polysorbate 1.2 1.2 1.2 1.2 1.2 1.2 Sorbitan stearate 0.5 0.5 0.5 0.5 0.5 0.5 Tocopheryl acetate 0.2 0.2 0.2 0.2 0.2 0.2 Cyclomethicone 3.0 3.0 3.0 3.0 3.0 3.0 BHT 0.05 0.05 0.05 0.05 0.05 0.05 C Incense, preservative Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount

Experimental Example  4: Moisturizing effect of bamboo powder extract

The clinical moisturizing effects of the cosmetic compositions of Formulation Examples 1, 2 and 3 were measured as follows. A, B and C groups were divided into 6 groups (A, B, C, D, E and F) by randomly dividing 120 healthy men and women who feel skin dry through a questionnaire. (Comparative Formulation Example 1) containing glycerin and 1,3-butylene glycol as moisturizing agents instead of the sugar alcohols extract, respectively, in Group D, and glycerin and sorbitol as moisturizing agents in Group E The cosmetic composition of the cosmetic composition (Comparative Formulation Example 2) and the cosmetic composition of the base formulation (Comparative Formulation Example 3) containing no moisturizing agent were applied to the F group twice a day for four weeks respectively. The moisturizing effect was evaluated by Corneometer CM 820 (Corage + Khazaka, Germany) for every 2 weeks and after the start of the test. The experimental results are shown in Table 5 below.

division Before use After 2 weeks of use After 4 weeks of use Formulation Example 1 40.6 56.3 60.3 Formulation Example 2 41.4 57.1 59.7 Formulation Example 3 40.8 58.3 59.3 Comparative Formulation Example 1 41.5 59.3 62.5 Comparative Formulation Example 2 40.7 56.7 61.2 Comparative Formulation Example 3 41.3 45.3 46.8

As shown in the above Table 6, the moisturizing effect of the cosmetic compositions (Formulation Examples 1, 2 and 3) containing the extract of the present invention of the present invention is also superior to the cosmetic compositions containing the other moisturizing agents (Comparative Formulations 1, 2 and 3) And it was confirmed that it is excellent.

Experimental Example  5: Antiseptic effect of bamboo shoot extract

In order to examine the antimicrobial effect of the extract of Pakjuuri, the cosmetics of Formulations 4 to 6 containing the Pakjuuri extract of Preparation Example 3 in the composition of the following Table 4, the cosmetic of Comparative Formulation Example 4 containing purified water instead of the Pakjuuri extract, The cosmetic composition of Comparative Formulation Example 5 containing methylparaben and imidazolidinyl urethane as antiseptics instead of the pearl millet extract was prepared.

ingredient Formulation Example Comparative Formulation Example 4 5 6 4 5 Content (unit:% by weight) Pumpkin extract
(Production Example 3) 0.5 1.0 2.0 - - glycerin 4.0 4.0 4.0 4.0 4.0 Butylene glycol 2.0 2.0 2.0 2.0 2.0 Propylene glycol 2.0 2.0 2.0 2.0 2.0 EDTA-2Na 0.02 0.02 0.02 0.02 0.02 Polysorbate 60 1.0 1.0 1.0 1.0 1.0 Glyceryl stearate 1.5 1.5 1.5 1.5 1.5 Wax 4.0 4.0 4.0 4.0 4.0 Macadamia nut oil 5.0 5.0 5.0 5.0 5.0 Squalane 3.0 3.0 3.0 3.0 3.0 Spices a very small amount a very small amount a very small amount a very small amount a very small amount Methylparaben - - - - 0.2 Imidazolidinyl urea - - - - 0.2 Purified water To 100 To 100 To 100 To 100 To 100

The cosmetic effects of the formulation examples and comparative formulations of Table 6 were used to measure the preservative effect against bacteria and fungi.

First, in the case of bacteria, 20 to 30 g of the cosmetic of Formulation Example 4-6 and Comparative Formulation Examples 4 and 5 were mixed with 20 g of Escherichia coli ; ATCC 8739), Pseudomonas aeruginosa (ATCC 9027) and Staphylococcus aureus (ATCC 6538) were added and mixed at an initial concentration of 10 ⁷ cfu / g per sample. 1 g of each cosmetic was taken at 1, 7, 14, 21 and 28 days intervals while culturing them in a thermostat at 30-32 ° C for 4 weeks, and the number of viable cells was measured. The measurement results are shown in Table 8 below.

Next, in the case of mold, Candida albicans ( Candida albicans ; ATCC 10231), Penicillium citrinum ; KCTC2123) and Aspergillus niger (ATCC16404) were added at a concentration of 10 ⁷ cfu / g per sample and cultured at 25 ° C in a thermostatic bath at intervals of 7 days, And the results are shown in Table 7 below.

-: No spawning and mycelium spawning for 8 weeks and good

+: Molding on the wall or lid within 4 weeks

++: mending within 4 weeks and mold on part of the surface

+++: Mildew and mold on the entire surface within 4 weeks

As shown in Table 7, the cosmetic composition of Comparative Example 4, which did not use the preservative, showed molds on the surface and the entire surface within 4 weeks, and the bacterial counts were remarkably increased even after 1 day. However, the cosmetic compositions of Formulation Examples 4 to 6 containing the peanut meal extract of the present invention exhibited a concentration-dependent antimicrobial effect on bacteria and fungi. After about 28 days, the cosmetic composition exhibited a concentration of 2.0% (w / v ), A repellency similar to or better than Comparative Formulation Example 5 in which methylparaben, which is a preservative, and imidazolidinyl urea were mixed was obtained, and when it was judged based on the whole culture day, when it was used in a content of 2.0% or more It was found that a far superior buoyancy was obtained as compared to Comparative Formulation Example 5 in which the preservative methylparaben and imidazolidinyl urea were mixed.

Experimental Example  6. Skin wrinkle improvement effect of Pusan juice extract

Clinical trials of the skin wrinkle-reducing effect of the cosmetic composition containing the extract of the present invention of the present invention were carried out. Clinical trials were conducted through actual use tests of each cosmetic product.

That is, the clinical test was carried out in the same manner as in Example 1 except that Formulation Example 1, which contained the bananas extract of Preparation Example 1, and Cream Cosmetic Composition (Comparative Formulations 6 and 7), which was replaced with adenosine 3% (v / v) , The effect of improving the skin wrinkles due to the use of cosmetics was confirmed and compared.

Forty women were divided into 20 experimental groups, each treated with cream (Comparative Formulation Example 6) in which adenosine was replaced with 3% (v / v) instead of pistachio extract, and in the experimental group of Formulation Example 1 And the wrinkle improvement effect after 8 weeks was visually evaluated using both sides of the face to check the degree of wrinkle improvement. The results of the wrinkle-improving effect based on this evaluation are shown in Table 8 below.

The wrinkle improvement effect of each subject before and 8 weeks after the completion of the experiment was evaluated by a skilled physician's naked eye and instrument measurement (Replica & Skin visiometer, C + K Electronic Co., Germany) , And the experimental results are shown in Table 8 below.

Device Rating
(Wrinkle depth) Improvement rate
(%) Visual evaluation Effective rate
(%) Before use after use Great slightly none Formulation Example 1
(Extract of bamboo shoots) 21.26 19.26 9.4 16 2 2 80.0 Comparative Formulation Example 6
(Adenosine) 21.48 20.19 6.0 17  2 One  85.0

As can be seen from the results of Table 8, similar results were obtained with the cosmetic composition of Comparative Example 6 containing adenosine, which is known to have a wrinkle-reducing effect instead of the brewer's berry extract, showing excellent wrinkle-reducing effect of the brewer's berry extract. In addition, skin irritation could not be observed in most of the subjects who applied the cosmetic composition containing the extract of the present invention to the skin.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the same is by way of illustration and example only and is not to be construed as limiting the scope of the present invention. It is therefore intended that the scope of the invention be defined by the claims appended hereto and their equivalents.

Claims (7)

A cosmetic composition for antioxidation containing an antioxidant extract as an active ingredient. A cosmetic composition for preventing skin aging, which comprises an extract of Pakjuri extract as an active ingredient. A cosmetic composition for improving the wrinkles of skin, which contains an extract of Panax notoginseng as an active ingredient. A cosmetic composition for moisturizing which contains an extract of Panax notoginseng as an active ingredient. The cosmetic composition according to any one of claims 1 to 3, The cosmetic composition according to any one of claims 1 to 5, wherein the saccharide extract is contained in an amount of 0.001 to 30.0% by weight based on the total weight of the cosmetic composition. 6. The method according to any one of claims 1 to 5, wherein the saccharide extract is selected from the group consisting of water, an anhydrous or a lower alcohol having 1-4 carbon atoms, propylene glycol, butylene glycol, glycerin, acetone, ethyl acetate, chloroform, Is extracted with a solvent extraction method or an ultrasonic extraction method using an extraction solvent selected from the group consisting of diethyl ether, dichloromethane, hexane, and mixtures thereof.