KR20150045010A - Cosmetic composition comprising the extract of Allium hookeri as active ingredient - Google Patents

Abstract

The present invention relates to a cosmetic composition including extracts of Allium hookeri as an active ingredient wherein 0.0001-30.0 wt% of the extract of Allium hookeri is included as an active ingredient for the total weight percentage of the cosmetic composition. In the present invention, the extract of Allium hookeri has a collagen biosynthesis effect, an antiseptic effect and an anti-aging skin effect and other effects of inhibiting the creation of MMP-1 and melanin, improving wrinkles and elasticity on skin and keeping moisture on skin, thereby having outstanding effects when being used in cosmetic compositions.

Description

[0001] The present invention relates to a cosmetic composition comprising an extract of Allium japonica as an active ingredient,

The present invention relates to a cosmetic composition comprising a tubular extract as an active ingredient.

A variety of physical and chemical changes occur in human skin during the aging process. The cause of this phenomenon is divided into intrinsic aging and photo-aging. Free radicals can be caused by activation of ultraviolet rays, stress, disease state, environmental factors, wounds, and aging. When such state is deepened, the antioxidant defense network existing in the living body is destroyed, And aging.

More specifically, lipids, proteins, polysaccharides and nucleic acids, which are major components of the skin, are oxidized to destroy skin cells and tissues, resulting in skin aging.

In particular, the oxidation of proteins can cause collagen (collagen), hyaluronic acid, elastin, proteoglycan, fibronectin, and the like that are severely hyperinflammatory reactions and skin elasticity If this gets worse, DNA mutations can lead to mutations, cancer, and immune deficiency.

Therefore, it is necessary to protect the cell membrane by destroying the free radicals mediated by free radicals, ultraviolet rays, and inflammation that occur during the metabolism of the body, and to regenerate already damaged cells by active metabolism, Can quickly recover and maintain healthy skin.

Aging involves not only these free radicals, but also enzyme called matrix metalloproteinase (MMP), a collagenase. That is, synthesis and degradation of extracellular matrix such as collagen in vivo are appropriately controlled, but collagen synthesis is reduced as aging progresses, and expression of matrix metalloproteinase (MMP), an enzyme that degrades collagen, is promoted, And the wrinkles are formed. These degrading enzymes are also activated by ultraviolet irradiation.

Therefore, there is a demand for development of a substance capable of modulating MMP expression induced in the cell or inhibiting its activity. Until now, the raw materials used as cosmetic materials mostly inhibited only the enzyme activity. Therefore, we tried to control the amount and activity of MMP expression induced by intracellular natural aging and photoaging.

On the other hand, skin changes due to pigment deposition occur. The factors that determine skin color are basically the partial or total pigmentation such as stain, freckle and tanning due to ultraviolet exposure, and the distribution of acne and scars and keratin Blood circulation, stress, and health. The most important factor among these factors is pigmentation.

Melanin, melanoid, carotene and hemoglobin are the most important factors affecting skin color. Among them, melanin is the most important factor affecting the biosynthesis of melanin.

Melanin absorbs or scatters ultraviolet light and plays a major role in preventing damage to the skin from ultraviolet rays. It does not have a specific maximum absorption wavelength and absorbs light in all areas. In addition, it has excellent ability to remove reactive oxygen species, sometimes melanin itself generates active oxygen, and other substances are reduced or oxidized by catechol or quinone in the melanin structure, and melanin itself shows free radical properties Pray.

The biosynthesis of melanin begins with the conversion of tyrosine, an amino acid, into melanosomes of melanocyte-forming melanosomes by tyrosinase and conversion to dihydroxy phenylalanine, followed by a series of oxidation processes to form pheomelanin, eumelanin.

This biosynthesis process is carried out in a special form of melanocyte in the brown intracellular organelle. The melanomas, including melanin granules, migrate from the nucleus to the tip of the dendritic process, and migrate into the cytoplasm by phagocytosis of keratinocytes. It accumulates around the nucleus.

The synthesis of melanin, the number of melanosomes, and the migration to keratinocytes in the periphery are partially affected by hormones and ultraviolet rays, and are primarily affected by genetics. In addition, it is known that interleukins, prostaglandins, histamines, and the like are involved in the expression of tyrosinase and cytokine, which is an intracellular regulator involved in the synthesis and transmission of melanin, and metal ions such as copper, zinc and iron.

(Japanese Patent Laid-open No. 4-9320), hydroquinone (Japanese Patent Laid-Open No. 192062), kojic acid (Japanese Patent Laid-open No. 56-7710), arbutin Activity, and is used as a whitening cosmetic. However, since it is low in stability in cosmetic formulations, its use is restricted due to decomposition and coloring, generation of odor, efficacy at a living body level, unclear effect and safety problem. These substances have proved their tyrosinase inhibitory effect, but their effect is lower in experiments similar to actual living body level. Therefore, it is required to evaluate the inhibitory effect by the melanoma cell culture similar to the biological level. Hydroquinone is also defined as a carcinogenic substance and its use in cosmetics is prohibited or restricted. Kojic acid and ascorbic acid are very unstable substances. When a cosmetic containing a small amount of the above components is kept at room temperature for several weeks, browning occurs.

In order to solve these various problems, recently, many cosmetics using natural products have been developed to reduce skin irritation caused by various chemical substances and the like. Natural materials are not only less harmful to the skin, but also have increased their value as a raw material for cosmetics due to the recent popularity of cosmetics using natural materials.

On the other hand, various substances such as water, oil, surfactant, protein, and plant extract are mixed in cosmetics, and microorganisms may multiply when the microorganisms are introduced under proper environment. Microbial contamination of cosmetics can degrade product quality and seriously harm consumers' health. In order to prevent such contamination, cosmetics use an appropriate amount of preservative such as paraoxybenzoic acid ester, phenoxyethanol, imidazolidinyl urea, and methyl isothiazolinone. However, these synthetic preservatives have been reported to cause skin irritation such as irritant contact dermatitis and allergic dermatitis accompanied by erythema, edema, swelling, and palsy, and thus there is a growing interest in natural preservatives using natural products.

The present inventors have studied the applicability of various natural products not yet known to cosmetics. As a result, the present inventors have selected a mung bean as a herb and a plant extract to produce an extract, thereby inhibiting MMP-1 production, collagen biosynthesis, As a result of measuring the inhibitory effect, the preservative effect, the moisturizing effect, the skin wrinkle improving effect, the skin elasticity improving effect and the skin aging effect, it was found that the efficacy as a cosmetic product can be expected.

It is an object of the present invention to provide a cosmetic composition which contains a tangerine extract and exhibits an effect of inhibiting MMP-1 production, collagen biosynthesis effect, melanin formation inhibitory effect, preservative effect, moisturizing effect, skin wrinkle improving effect, skin elasticity improving effect, .

It is also an object of the present invention to provide a cosmetic composition that exhibits efficacy by preparing supercritical, ultrasonic, and fermented extracts, and purifying the extracts to maximize the efficacy.

Therefore, the object of the present invention as described above is achieved by a cosmetic composition comprising a tubular extract as an active ingredient.

Preferably, the cosmetic composition is for improving skin wrinkles.

Further, preferably, the cosmetic composition is characterized by being for skin whitening.

Preferably, the cosmetic composition is a preservative.

Further, preferably, the cosmetic composition is characterized in that it is for moisturizing the skin.

Preferably, the cosmetic composition is for improving skin elasticity.

Preferably, the cosmetic composition is characterized in that it is for preventing skin aging.

Preferably, the tubular extract is contained in an amount of 0.0001 to 30.0% by weight based on the total weight of the cosmetic composition.

Preferably, the above-mentioned tubular extract comprises (a) water, an anhydrous or a lower alcohol having 1-4 carbon atoms, propylene glycol, butylene glycol, glycerin, acetone, ethyl acetate, chloroform, butyl acetate, diethyl ether, dichloro (B) a supercritical extraction method, (c) an ultrasonic extraction method and / or (d) a fermentation extraction method, wherein the solvent extraction method is an extraction method using an extraction solvent selected from the group consisting of methanol, hexane and mixtures thereof.

Preferably, the cosmetic composition is a cosmetic composition exhibiting an effect of inhibiting MMP-1 production, collagen biosynthesis effect, melanin formation inhibitory effect, preservative effect, moisturizing effect, skin wrinkle improving effect, skin elasticity improving effect, .

Preferably, the cosmetic composition is a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, cleansing oil, powder foundation, emulsion foundation, wax foundation, pack, massage cream and spray Lt; RTI ID = 0.0 > of: < / RTI >

Other objects of the present invention will be described in detail with reference to the following detailed description and drawings.

The triacylglycerol extract according to the present invention is effective for inhibiting MMP-1 production (Experimental Example 1), enhancing collagen synthesis (Experimental Example 2), inhibiting melanin production (Experimental Example 3), skin moisturizing effect (Experimental Example 4) (Experimental Example 5), skin wrinkle improving effect (Experimental Example 6) and skin elasticity improving effect (Experimental Example 7).

In addition, according to the present invention, in order to maximize the efficacy of the tubercle leaf extract, the effect of suppressing MMP-1 production, the effect of promoting collagen synthesis, the effect of inhibiting melanogenesis, the effect of improving skin elasticity, etc. can be obtained through ultrasound, supercritical extraction and / .

The triacylglycerols (sammi, jumit, and roots) used as active ingredients in the cosmetic composition of the present invention include Allium It is labeled as hookeri . It is a perennial primrose of lily and wing. It is similar to the orange-yellow wave around 8-9. It is native to high altitudes of more than 1,400 meters in the Himalayas and is distributed in India, China, Bhutan, Sri Lanka and Myanmar. It is called "三 菜" because it has sweetness, bitter taste, and pungent taste. It is also called "玄 菜" because it is called "ginseng".

In the present invention, the tubular extract means an extract obtained from various organs or parts (for example, leaves, flowers, roots, stems, etc.) of a tub, preferably an extract obtained from a plant or root, ≪ / RTI >

In addition, the tubular extract of the present invention can be prepared by using conventional solvents known in the art, that is, under the conditions of ordinary temperature and pressure, but preferably (a) water, Propanol and butanol), propylene glycol, 1,3-butylene glycol, glycerin, acetone, diethyl ether, ethyl acetate, butyl acetate, dichloromethane (such as methanol, ethanol, (B) solvent extraction using a solvent selected from the group consisting of chloroform, hexane and mixtures thereof; (b) supercritical extraction with carbon dioxide and supercritical extraction with high temperature; or (c) extraction with ultrasonic extraction.

In the present invention, a solvent extraction method using an extraction solvent is preferably used.

In the present invention, the tangerine extract may be obtained from various extraction solvents, for example, water, anhydrous or lower alcohols having 1-4 carbon atoms (e.g., methanol, ethanol, propanol and butanol), propylene glycol, And at least one extraction solvent selected from the group consisting of glycerin, acetone, diethyl ether, ethyl acetate, butyl acetate, dichloromethane, chloroform, hexane and mixtures thereof. (v / v) ethanol or water, and most preferably 70% (v / v) ethanol.

On the other hand, it is obvious to those skilled in the art that the triple extract of the present invention can be obtained not only by the above-mentioned extraction solvent but also by using other extraction solvent, which exhibits substantially the same effect.

In addition, the tubular extract of the present invention includes not only the extract obtained by the above-described extraction solvent, but also the extract obtained through ordinary purification and fermentation processes. For example, decompression by carbon dioxide, extraction by supercritical extraction with high temperature, extraction by extraction using ultrasound, separation by ultrafiltration membrane with constant molecular weight cutoff value, various chromatography (size, charge, hydrophobic or affinity And the active fraction obtained through various purification and extraction methods, such as an extract obtained by fermentation products using natural or various microorganisms, are also included in the extract of the present invention.

Further, the present invention provides a cosmetic composition characterized in that the above extract is obtained by liquid-freezing obtained by cold-pressing and heating filtration at room temperature, and further by concentrating or lyophilizing the solvent under reduced pressure.

The supercritical fluid extraction refers to supercritical fluid extraction. Generally, the supercritical fluid is extracted from the liquid and the liquid when the gas reaches the critical point at high temperature and high pressure. (J. Chromatogr. A. 1998; 479: 200-205). In addition, it has been reported that the supercritical fluid has a polarity similar to that of a nonpolar solvent.

Carbon dioxide is a supercritical fluid with both liquid and gaseous nature, with the operation of supercritical fluid equipment reaching its critical pressure and temperature, resulting in increased solubility in fat-soluble solutes. When supercritical carbon dioxide passes through an extraction vessel containing a certain amount of sample, the lipophilic substance contained in the sample is extracted into supercritical carbon dioxide.

When the supernatant carbon dioxide containing a small amount of cosolvent is passed through the sample remaining in the extraction vessel after extracting the lipid-soluble substance, components that were not extracted by pure supercritical carbon dioxide can be extracted.

The supercritical fluid used in the supercritical extraction method of the present invention can effectively extract an active ingredient by using a mixed fluid in which a cosolvent is further mixed with supercritical carbon dioxide or carbon dioxide.

These co-solvents may be used alone or in admixture of two or more selected from the group consisting of chloroform, ethanol, methanol, water, ethyl acetate, hexane and diethyl ether.

Most of the extracted samples contain carbon dioxide. Since the carbon dioxide is volatilized into air at room temperature, the extract obtained by the above method can be used as a cosmetic composition, and the cosolvent can be removed by a reduced pressure evaporator.

In addition, the ultrasonic extraction method is an extraction method using energy generated by ultrasonic vibration. Ultrasonic waves can destroy an insoluble solvent contained in a sample in a water-soluble solvent. Due to the high local temperature generated at this time, Since the kinetic energy of the particles is increased, sufficient energy required for the reaction is obtained. By inducing the high pressure by the shock effect of the ultrasonic energy, the mixing efficiency of the substance contained in the sample and the solvent is increased, thereby increasing the extraction efficiency.

As the extraction solvent which can be used for the ultrasonic extraction method, one or a mixture of two or more selected from the group consisting of chloroform, ethanol, methanol, water, ethyl acetate, hexane and diethyl ether can be used. The extracted sample is recovered by vacuum filtration, and the filtrate is recovered, and the extract is removed by a vacuum evaporator and freeze-dried to obtain an extract.

The tubular extract according to the present invention also includes an extract obtained through fermentation, and the fermented extract of the trifoliate can be prepared as follows. After finely crushing the trash to about 100 to 500 mesh, 1 to 50 g / L of a conventional microorganism culture solution is added, and microorganisms such as yeast strains or lactic acid bacteria are added in an amount of 10,000 to 100,000 cfu / L. The culture temperature is 30 to 37 ° C. The pH is 5 to 7 and aerobic or anaerobic conditions are maintained for about 5 to 10 days. Which can be obtained through aging and filtration.

According to the present invention, the tubular extract is contained in an amount of 0.0001 to 30.0% by weight based on the total weight of the cosmetic composition, more preferably the tubular extract is contained in an amount of 0.01 to 10% by weight based on the total weight of the cosmetic composition do. When the content of the extract is less than 0.0001% by weight, the effect of improving the skin is not exhibited. When the content of the extract is more than 30.0% by weight, the degree of improvement of the skin against the increase of the content is insignificant, It is not even.

Therefore, the cosmetic composition of the present invention comprising the triple extract having various functions of the present invention has excellent collagen synthesis promoting effect, MMP-1 production inhibiting effect, skin wrinkle improving effect, skin elasticity improving effect, skin whitening effect, Preservative effect, moisturizing effect, and skin aging prevention effect.

The ingredients contained in the cosmetic composition of the present invention may contain, as an active ingredient, the ingredients commonly used in cosmetic compositions in addition to the above-mentioned effective ingredients, and may contain conventional ingredients such as antioxidants, stabilizers, solubilizers, vitamins, Supplements, and carriers.

The cosmetic composition of the present invention can be prepared into any of the formulations conventionally produced in the art and can be used in the form of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, , Oil, powdered foundation, emulsion foundation, wax foundation, pack, massage cream and spray, but is not limited thereto. More specifically, it can be manufactured in the form of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder.

When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

In the case where the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide ether Alkylamido betaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester.

When the cosmetic composition of the present invention is a soap, a surfactant-containing cleansing formulation or a surfactant-free cleansing formulation, it may be applied to the skin and then wiped off, washed or rinsed with water. The surfactant-containing cleansing formulation may be a cleansing foam, a cleansing water, a cleansing towel and a cleansing pack. The surfactant-free cleansing formulation may be a cleansing cream, , Cleansing lotion, cleansing water and cleansing gel, but is not limited thereto.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are only for describing the present invention in more detail and that the scope of the present invention is not limited by these embodiments in accordance with the gist of the present invention .

Production Example 1: Preparation of tubular extract

The shrubs of the shrubs were shaken out three times for 5 hours in a 70% (v / v) aqueous ethanol solution, cooled down, and filtered through a Whatman # 4 filter paper. The filtered extract was concentrated under reduced pressure and freeze-dried at 50 DEG C or lower.

Manufacturing example  2 to 3. Supercritical  Manufacture of fluid extracts and ultrasonic extracts

The supercritical extract was obtained by extracting with a conventional supercritical extraction method (extracting with ethanol as a co-solvent in a supercritical state under a pressure of about 300 atm at a temperature of about 60 DEG C). Extracts were obtained by extraction using ultrasound (Super Sonic Ultrasonic Device at 25 ° C for 2 hours at 30 ° C). In the case of Production Example 3, ethanol was used as a co-solvent.

Experimental Example  One. Triptych  Extract MMP -1 production inhibitory effect

In Experimental Example 1, the effect of inhibiting the production of MMP-1, a collagenase, by the extract of the tubers obtained in Production Examples 1, 2 and 3 was measured.

Human normal skin cells were inoculated into a 48-well microplate (Nunc, Denmark) at a concentration of 1 × 10 ⁶ cells per well and cultured in DMEM medium (Sigma, USA) and 37 ° C For 24 hours. Then, the cells were further cultured in serum-free DMEM medium supplemented with the tubular extracts of Preparation Examples 1, 2 and 3 and in a serum-free DMEM medium containing no triple extract for 48 hours.

After the incubation, the supernatant of each well was collected and the amount (ng / ml) of the newly synthesized MMP-1 was measured using an MMP-1 assay kit (Amersham, USA) The inhibition rate (%) was calculated and the results are shown in Table 1.

Name of sample Treatment concentration (Production Example 1)
Inhibition rate of MMP-1 production (%) (Production Example 2)
Inhibition rate of MMP-1 production (%) (Production Example 3)
Inhibition rate of MMP-1 production (%) Triple extract
10ppm 22.79 24.17 25.82 50 ppm 35.23 37.92 38.89 100ppm 51.34 54.81 56.14 500ppm 76.25 78.23 79.53 1000ppm 87.08 89.89 90.21 TGF-beta (200 nM) 76.8

As shown in the results of Table 1, it was confirmed that the tubular extract of the present invention inhibits MMP-1 production (inhibits MMP-1 activity) in a concentration-dependent manner. Even considering the difference between the concentration of TGF-beta (200 nM), which is a substance having an inhibitory effect on MMP-1 production, and the concentration (500 ppm) of the triplex extract of the present invention, Showed that MMP-1 inhibitory effect of the present invention was inhibited by MMP-1.

Experimental Example 2. Enhancement of Collagen Synthesis Effect of Samjuk Extract

Human normal epithelial cells, fibroblasts, were inoculated on a 48-well microplate at a density of 1 × 10 ⁶ cells per well, and cultured in DMEM medium for 24 hours. Serum-free DMEM medium supplemented with the tubercle extract prepared in Preparation Examples 1, 2 and 3 and the control group were further cultured for 48 hours in serum-free DMEM medium containing no triple extract. After the incubation, the supernatant of each well was collected, and the amount of procollagen type I C-peptide (PICP) was measured using a collagen kit (Takara, Japan) and converted into ng / Respectively. Collagen biosynthesis increase rate (%) was calculated according to the following formula (2), and the results are shown in Table 2.

Name of sample Treatment concentration (Production Example 1)
Collagen biosynthesis rate (%) (Production Example 2)
Collagen biosynthesis rate (%) (Production Example 3)
Collagen biosynthesis rate (%) Triple extract 2.5 ppm 11.5 13.5 12.8 5 ppm 19.4 22.5 20.1 10ppm 40.3 47.9 45.1 25 ppm 94.8 102.1 97.5 50 ppm 158.4 168.5 160.1 TGF-beta (200 nM)  165.5

According to Table 2, it was confirmed that the collagen biosynthesis rate of the purified triacylgia extract prepared in Production Examples 1, 2, and 3 of the present invention increased in a concentration-dependent manner. In particular, it was confirmed that the extract prepared in Preparation Example 2 was more effective than the extracts prepared in Preparation Examples 1 and 3.

Experimental Example  3: Triptych  Inhibitory Effect of Extracts on Melanin Production by B16F1 Melanocytes

In Experimental Example 3, in order to confirm the whitening effect of the triplex extracts obtained in Production Examples 1, 2 and 3, the degree of inhibition of melanin formation on B16F1 melanocyte-forming cells was examined to determine the whitening effect. The B16F1 melanin-forming cell used in Experimental Example 3 is a cell strain derived from a mouse, and is a cell that secretes a melanin pigment called melanin.

During the artificial culture of these cells, samples were treated to compare the degree of reduction of melanin pigment. The B16F1 melanin-forming cells used in this experiment were purchased from the American Type Culture Collection (ATCC).

Melanin biosynthesis inhibitory effect of B16F1 melanin-forming cells was measured as follows.

B16F1 melanocyte-forming cells were divided into 6-well plates at a concentration of 2 × 10 ⁵ cells per well. After incubating the cells, the cells were treated for 72 hours with the triple extract according to Preparation Examples 1 to 3 at a concentration not causing toxicity. After incubation for 72 hours, the cells were detached with trypsin-EDTA, the number of cells was measured, and the cells were recovered by centrifugation. Quantification of intracellular melanin was carried out with a slight modification of the method of Lotan (Cancer Res., 40: 3345-3350, 1980). Cell pellet was washed once with PBS, and 1 ml of homogenization buffer solution (50 mM sodium phosphate, pH 6.8, 1% Triton X-100, 2 mM PMSF) was added and vortexed for 5 minutes to disrupt the cells. After centrifugation (3,000 rpm, 10 min), 1N NaOH (10% DMSO) was added to the cell filtrate to dissolve the extracted melanin, and the absorbance of melanin was measured at 405 nm with a microplate reader. The inhibition rate (%) of melanin formation was measured. Melanin formation inhibition rate (%) of B16F1 melanin-forming cells was calculated by the following equation (3), and the results are shown in Table 3 below.

A: Amount of melanin in wells to which no sample was added

B: Amount of melanin in the well to which the sample was added

Name of sample Treatment concentration (%) Melanin formation inhibitory effect (%) Production Example 1 Production Example 2 Production Example 3 The tubular extract (Preparation Example 2)
0.001 22.17 19.27 23.11 0.005 39.55 37.89 42.16 0.01 54.73 53.72 56.42 0.05 74.66 74.11 77.42 Arbutin 200ppm  68.25

As can be seen from the results of Table 3, it was found that the purified tripeak extracts prepared according to Production Examples 1, 2 and 3 of the present invention significantly inhibited melanin production in B16F1 melanin-forming cells.

Formulation Example 1: Preparation of cosmetic composition containing tubular extract

The cosmetic compositions containing the tubular extracts were prepared in the same manner as the cosmetic preparations containing the tubular extracts of Preparation Examples 1 to 3 (Formulation Examples 1, 2 and 3). For comparison of efficacy, glycerin and 1,3- (Comparative formulation example 1) containing glycerin and sorbitol as a moisturizing agent (comparative formulation example 2), and a cosmetic composition (comparative formulation example 3) containing neither an extract nor a moisturizer were prepared as follows: The results are shown in Table 4 (content unit: wt%).

ingredient Formulation Example 1 Formulation Example 2 Formulation Example 3 Comparative Formulation Example 1 Comparative Formulation Example 2 Comparative Formulation Example 3 Triple extract
(Production Example 1) 5.0 - - - - Triple extract
(Production Example 2) - 5.0 - - - Triple extract
(Production Example 3) 5.0 glycerin 5.0 5.0 1,3-butylene glycol - - 5.0 - - Sorbitol - - - 5.0 - EDTA-2Na 0.02 0.02 0.02 0.02 0.02 0.02 Purified water to 100 to 100 to 100 to 100 to 100 to 100 Cetostearyl alcohol 2.0 2.0 2.0 2.0 2.0 2.0 Glyceryl stearate 1.5 1.5 1.5 1.5 1.5 1.5 Microcrystalline 0.7 0.7 0.7 0.7 0.7 0.7 Squalane 5.0 5.0 5.0 5.0 5.0 5.0 Liquid paraffin 3.0 3.0 3.0 3.0 3.0 3.0 Trioctanoin 5.0 5.0 5.0 5.0 5.0 5.0 Polysorbate 1.2 1.2 1.2 1.2 1.2 1.2 Sorbitan stearate 0.5 0.5 0.5 0.5 0.5 0.5 Tocopheryl acetate 0.2 0.2 0.2 0.2 0.2 0.2 Cyclomethicone 3.0 3.0 3.0 3.0 3.0 3.0 BHT 0.05 0.05 0.05 0.05 0.05 0.05 Incense, preservative Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount

Experimental Example  4 : Triptych  Moisturizing effect of extract

The clinical moisturizing effects of the cosmetic compositions of Formulation Examples 1, 2 and 3 were measured as follows. A, B, and C groups were divided into five groups (A, B, C, D, and E) by a randomly selected group of 25 healthy adult men and women who felt skin dry through the questionnaire. (Comparative Formulation Example 1) containing glycerin and 1,3-butylene glycol as moisturizing agents, a cosmetic composition comprising glycerin and sorbitol as a moisturizing agent (Comparative Example 1 2) cosmetic compositions were applied to the face for 2 weeks each for 8 weeks. The moisturizing effect was evaluated by Corneometer CM 820 (Corage + Khazaka, Germany) for every 2 weeks and after the start of the test. The experimental results are shown in Table 5 below.

Skin moisturizing effect of water extract division Before use After 2 weeks of use After 4 weeks of use Formulation Example 1 23.6 36.5 45.1 Formulation Example 2 23.8 38.1 49.8 Formulation Example 3 24.1 38.9 51.9 Comparative Formulation Example 1 24.3 37.1 45.9 Comparative Formulation Example 2 23.5 35.3 46.7 Comparative Formulation Example 3 23.7 24.1 23.9

As shown in Table 5, it was found that the moisturizing effect of the cosmetic compositions (Formulation Examples 1, 2 and 3) containing the tubular extract of the present invention was superior to the cosmetic compositions containing other moisturizing agents (Comparative Formulation Examples 1 and 2) I could confirm.

Experimental Example  5: Triptych  Preservative effect of extract

In order to examine the preservative effect of the tubular extract, the cosmetic composition of Formulation Example 4-11 containing the tubular extracts of Preparation Examples 1, 2 and 3 at various ratios in the composition of the following Table 6, the comparative formulation containing purified water instead of the tubular extract The cosmetic composition of Comparative Formulation Example 5 containing methylparaben and imidazolidinyl urethane as an antiseptic instead of the cosmetic composition of Example 4 was prepared (unit: wt%).

ingredient Formulation Example Comparative Formulation Example 4 5 6 7 8 9 10 11 4 5 Triple extract
(Production Example 1) 0.5 1.0 2.0 - - - - - - Triple extract
(Production Example 2) - - - 0.5 1.0 2.0 - - - - Triple extract
(Production Example 3) 0.5 1.0 glycerin 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.0 Butylene glycol 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 Propylene glycol 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 EDTA-2Na 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 Polysorbate 60 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 Glyceryl stearate 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 Wax 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.0 Macadamia nut oil 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 Squalane 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 Spices a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount Methylparaben - - - - - - - - - 0.2 Imidazolidinyl urea - - - - - - - - - 0.2 Purified water To 100 To 100 To 100 To 100 To 100 To 100 To 100 To 100 To 100 To 100

The cosmetic effects of the formulation examples and comparative formulations of Table 6 were used to measure the preservative effect against bacteria and fungi.

First, in the case of bacteria, Escherichia coli (ATCC 8739), Pseudomonas aeruginosa (ATCC 9027), and Staphylococcus were added to the cosmetic compositions of Formulation Examples 4-11 and 20-30 g of Comparative Formulation Examples 4 and 5, Staphylococcus aureus (ATCC 6538) was added and mixed at an initial concentration of 10 ⁷ cfu / g per sample. 1 g of each cosmetic was taken at 1, 7, 14, 21 and 28 days intervals while culturing them in a thermostat at 30-32 ° C for 4 weeks, and the number of viable cells was measured. The measurement results are shown in Table 7 below.

Next, in the case of fungi, a mixed fungus of Candida albicans (ATCC 10231), Penicillium citrinum (KCTC2123) and Aspergillus niger (ATCC 16404) is added at a concentration of 10 ⁷ cfu / g, and the mixture was incubated at 25 ° C. in a thermostatic chamber, and the presence or absence of mycelia and spore formation were observed at intervals of 7 days. The results are shown in Table 7 below.

Cosmetics Bacteria (cfu / g) mold* Initial number of bacteria Day 1 Day 7 Day 14 Day 21 Day 28 Formulation Example 4 1 x 10 ⁷ 830000 40000 5000 500 <100 - Formulation Example 5 1 x 10 ⁷ 700000 15000 1000 250 <100 - Formulation Example 6 1 x 10 ⁷ 310000 4000 400 <100 <100 - Formulation Example 7 1 x 10 ⁷ 750000 20000 3500 400 <100 - Formulation Example 8 1 x 10 ⁷ 680000 10000 800 150 <100 Formulation Example 9 1 x 10 ⁷ 270000 3500 200 <100 <100 Formulation Example 10 1 x 10 ⁷ 710000 25000 2500 400 <100 - Formulation Example 11 1 x 10 ⁷ 650000 9000 700 300 <100 - Comparative Formulation Example 4 1 x 10 ⁷ 8800000 8100000 7000000 6300000 5800000 +++ Comparative Formulation Example 5 1 x 10 ⁷ 380000 5000 450 <100 <100 -

-: No spawning and mycelium spawning for 8 weeks and good

+: Molding on the wall or lid within 4 weeks

++: mending within 4 weeks and mold on part of the surface

+++: Mildew and mold on the entire surface within 4 weeks

As shown in Table 7, the cosmetic composition of Comparative Formulation Example 4, which did not use preservatives, showed molds on the whole surface and on the surface within 4 weeks, and bacterial bacteria were remarkably increased even after 1 day. However, the cosmetic compositions of Formulation Examples 4 to 11 containing the extract of the present invention showed a concentration-dependent preservative effect on bacteria and fungi, and after about 28 days, 2.0% (w / v) The preservation force similar to or better than Comparative Formulation Example 5 in which the preservative methylparaben and imidazolidinyl urea were mixed was secured. When the content of the preservative was judged to be 2.0% or more, In comparison with Comparative Example 5 in which methylparaben and imidazolidinyl urea were mixed with each other. In particular, the tubular extract prepared in Preparation Examples 2 and 3, rather than Preparation Example 1, .

Experimental Example  6. Triptych  Effect of extracts on skin wrinkles

Clinical trials of the skin wrinkle-reducing effect of the cosmetic composition containing the tubular extract of the present invention were conducted. Clinical trials were evaluated through actual use tests of each cosmetic product.

That is, the clinical test was conducted in the same manner as in the formulation example 2 containing the tuberculosis extract of Preparation Example 2 of Table 4 and the cosmetic composition of Comparative Formulation Example 1 containing glycerin and 1,3 butylene glycol as the humectant, The effect of improving the skin wrinkles due to the use of cosmetics was confirmed and compared.

Twenty patients (20 to 35 years old) were applied to Formulation Example 2 on the right side of the face and Comparative Formulation Example 1 on the left side of the face for 2 consecutive months, respectively.

(Comparative Formulation Example 1) treatment group containing glycerin and 1,3-butylene glycol as moisturizing agents, and 3% (v / v) adenosine extract were administered to 60 experimental women, (Comparative Formulation Example 6, no content was shown in Table 4), and the test group of Formulation Example 2, which contained a tricolor slurry, and wrinkle improvement effect after 6 weeks using both sides of the face Was visually evaluated to confirm the degree of wrinkle improvement. The results of the wrinkle-improving effect based on this evaluation are shown in Table 9 below.

The effect of improving wrinkles after the completion of the experiment was evaluated by visual examination and instrument measurement (cutometer SEM 575, C + K Electronic Co., Germany) of a skilled physician before and after 3 months of use. The experimental results are shown in Table 8 below.

Wrinkle-improving effect (visual evaluation and device evaluation) of a cosmetic composition containing a tubular extract Wrinkle improvement effect Effective rate (%) Great slightly none Formulation Example 2
(Samchae extract) 22 5 3 90.0 Comparative Formulation Example 1
(Moisturizer) 5 5 20 33.3 Comparative Formulation Example 6
(Adenosine) (20) (6) ( 4 ) (86.7)

As can be seen from the results of Table 8, the cosmetic composition of Formulation Example 2 containing the tubular extract of the present invention showed about 2.7 times higher wrinkle-improving effect than Comparative Formulation Example 1 containing moisturizing agent.

In addition, similar results were obtained with the cosmetic preparation of Comparative Example 6 containing adenosine, which is known to have a wrinkle-improving effect instead of the tubular extract, and it was confirmed that the tubular extract exhibits excellent wrinkle-reducing effect.

Experimental Example  7: Measurement of skin elasticity improvement effect

In order to examine the skin elasticity improvement effect of the cosmetic composition according to the formulation examples and the comparative formulation examples obtained in Production Examples 1, 2 and 3, Experimental Example 7 was applied to 40 Were measured. The composition of Example 1 was divided into four groups of 10, 10, 2, 3, and 4, and the cosmetic composition of Comparative Formulation Example 1 was administered every morning and evening The skin elasticity was measured 12 weeks after the application to the face for 2 weeks for 12 weeks. The elasticity of the facial region was measured using a cutometer SEM575 (MPA 580, Courage + Khazaka GmbH, Germany), and the R2 (biological elasticity) indicating skin elasticity was measured before and after application . The results are shown in Table 9 below, and the results are the average of the degree of improvement for each group.

Experimental product Skin elasticity improvement (%) Formulation Example 1 25.5 Formulation Example 2 26.7 Formulation Example 3 24.8 Comparative Formulation Example 1 2.5

As shown in Table 9, Formulation Examples 1, 2 and 3 containing the tubercle leaf extract significantly improved skin elasticity compared to the comparative formulation.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the same is by way of illustration and example only and is not to be construed as limiting the scope of the present invention. It is therefore intended that the scope of the invention be defined by the claims appended hereto and their equivalents.

Claims (9)

A cosmetic composition comprising a triple extract as an active ingredient. The cosmetic composition according to claim 1, wherein the cosmetic composition is for improving skin wrinkles. The cosmetic composition according to claim 1, wherein the cosmetic composition is for skin whitening. The cosmetic composition according to claim 1, wherein the cosmetic composition has a preservative effect. The cosmetic composition according to claim 1, wherein the cosmetic composition is for skin moisturizing. The cosmetic composition according to claim 1, wherein the cosmetic composition is for improving skin elasticity. The cosmetic composition according to claim 1, wherein the cosmetic composition is for preventing skin aging. The cosmetic composition according to any one of claims 1 to 7, wherein the triple extract is contained in an amount of 0.0001 to 30.0% by weight based on the total weight of the cosmetic composition. [Claim 7] The method according to any one of claims 1 to 7, wherein the tubular extract comprises (a) water, an anhydrous or a lower alcohol having 1-4 carbon atoms, propylene glycol, butylene glycol, glycerin, acetone, ethyl acetate, chloroform, (B) a supercritical extraction method, (c) an ultrasonic extraction method, and (d) a fermentation extraction method, wherein the solvent extraction method is a solvent extraction method using an extraction solvent selected from the group consisting of methyl acetate, ethyl acetate, &Lt; / RTI &gt;