KR20200107444A - The skin cosmetic composition for anti-oxidation and anti-wrinkle of skin comprising natural complex extracts - Google Patents

Abstract

The present invention relates to a skin cosmetic composition for skin anti-oxidation and skin wrinkle alleviation containing a natural complex extract. More specifically, the present invention relates to a skin cosmetic composition for skin anti-oxidation and skin wrinkle alleviation containing a natural complex extract which is formed by mixing a mixed powder of Fagopyrum esculentum, Vigna radiata, Vigna angularis, Castanea crenata, Oryza sativa, persimmon leaves, and Angelica dahurica with extracts of Moschus moschiferus, Angelica gigas, Cornus officinalis, and Cervus nippon.

Description

The skin cosmetic composition for anti-oxidation and anti-wrinkle of skin comprising natural complex extracts}

The present invention relates to a skin cosmetic composition for skin antioxidant and wrinkle improvement containing a natural complex extract, and more particularly, in musk, angelica, cornus milk, deer antler extract, buckwheat, mung bean, red bean, yulpi, brown rice, persimmon leaves and white paper. It relates to a skin cosmetic composition for skin antioxidant and wrinkle improvement containing a natural complex extract formed by mixing mixed powder.

The cells of the human skin are subjected to oxidative stress by reactive oxygen species that occur in biochemical reactions that are constantly occurring to supply the necessary energy in the human body to supply the necessary energy in the human body. , Carbohydrate and DNA oxidative damage and enzyme activity are changed, causing various diseases such as skin cancer, and promoting aging.

In addition, photoaging caused by ultraviolet rays is the largest cause of external aging, which causes acute and chronic skin injuries such as skin cancer through continuous exposure to UV rays.

In this way, active oxygen introduced from the outside of the body or generated in the body causes many problems such as promoting aging in the living body or causing cancer. Therefore, development of an antioxidant substance that inhibits oxidation by active oxygen And a lot of research is being done.

Antioxidants are widely distributed in animals and plants, and many phenolic compounds, flavonoids, tocopherols, vitamin C, and selenium are known in fruits and vegetables. However, natural antioxidants cannot be expected to have a substantially sufficient effect when applied to the skin. Therefore, synthetic antioxidants that have excellent antioxidant power and are inexpensive are widely used, but their use is limited due to concerns about safety such as side effects on the human body.

On the other hand, the main functions of collagen, which are deeply related to the health of the skin, are known for the firmness of the skin, the resistance and bonding power of the tissue, and the support of cell adhesion. These collagens are known to be closely related to the formation of wrinkles in the skin due to aging and photoaging caused by UV irradiation.

In particular, retinoic acid, a substance that promotes collagen synthesis, is claimed that when collagen metabolism is activated by promoting the synthesis of collagen, the components of the dermal matrix increase, which can improve wrinkles, improve elasticity, strengthen skin, and heal wounds. ), TGF (transforming growth factor), animal-derived placenta, beturinic acid, chlorella extract, etc. have been released in many products, but have not received much attention.

In addition, many products containing collagen, which use collagen as a cosmetic composition for skin protection, are sold, but it is pointed out that cosmetics applied to the skin surface are difficult to expect skin moisturizing effects due to the difficulty of transdermal absorption of collagen, a polymer.

In addition, elastin fibers in the skin form cross-links inside the dermis (epiderm) together with collagen fibers, and are involved in skin elasticity. As the skin ages, histological deficiency and aggregation of elastin fibers, reduction of collagen fibers, increase in inflammatory cell infiltration, and biochemically remarkable increase in the activity of elastase, an elastin-degrading enzyme. Therefore, the elasticity of the skin decreases rapidly due to the action of elastase, and sagging occurs. Elastase is the only enzyme known to date that can break down elastin, and its inhibition can fundamentally reduce skin aging.

As a conventionally developed skin cosmetic composition for skin antioxidant and wrinkle improvement, the active ingredient is okra polysaccharide extracted from the fruit of mature okra (Hibiscus esculentus) with the skin removed in Korean Patent 10-1490762 (registration date February 2, 2015). Cosmetic compositions for antioxidants containing are known.

In addition, there is known a cosmetic composition for antioxidant, anti-wrinkle, anti-inflammatory and whitening consisting of a mixture extract of licorice, angelica and gosam in Korean Patent Publication 10-2015-0118293 (published on October 22, 2015).

In addition, the Korean Patent Registration 10-1758338 (registration date July 10, 2017) removes reactive oxygen species (ROS) and contains 1 to 4 mg/mL of aloe flower extract as an active ingredient, dermatitis, atopy , Hepatitis, diabetes, scleroderma, neurodegenerative diseases, atherosclerosis and ischemia, for preventing or treating one or more diseases selected from the group consisting of active oxygen inhibitory compositions are known.

In addition, in Korean Patent Registration No. 10-1574248 (registration date November 27, 2015), one or more parts selected from the group consisting of leaves, stems, roots, fruits, and seeds excluding flowers of Reynoutria sachalinensis were used with methanol. A cosmetic composition for improving skin wrinkles comprising the extracted extract as an active ingredient is known.

However, although the skin cosmetic compositions contain natural substances to exhibit antioxidant or wrinkle improvement effects, most of them use a single natural substance or some auxiliary natural substances, so that the antioxidant and wrinkle improvement effects are not satisfactory at the same time. .

Accordingly, as a result of the present inventors' efforts to solve the above problems, the natural complex extract formed by mixing the mixed powder of buckwheat, mung bean, red bean, yulk blood, brown rice, persimmon leaves and white paper in the extract of musk, angelica, cornus milk, and antler has an antioxidant effect. While having excellent anti-wrinkle effect and less side effects on the human body, a new skin cosmetic composition was developed and completed the present invention.

[Patent Document 1] Korean Patent Registration 10-1490762 (Registration Date February 2, 2015) [Patent Document 2] Korean Patent Publication 10-2015-0118293 (Publication date October 22, 2015) [Patent Document 3] Korean Patent Registration 10-1758338 (Registration Date July 10, 2017) [Patent Document 4] Korean Patent Registration 10-1574248 (Registration Date November 27, 2015)

In order to solve the above problems, the present invention is a skin antioxidant and wrinkle containing a natural complex extract formed by mixing a mixed powder of buckwheat, mung beans, red beans, yulpi, brown rice, persimmon leaves and white paper in an extract of musk, angelica, cornus milk, and antler It is a problem to be solved to provide an improvement skin cosmetic composition.

The present invention provides a skin cosmetic composition for skin antioxidant and wrinkle improvement comprising a natural complex extract formed by mixing mixed powders of buckwheat, mung bean, red bean, yulpi, brown rice, persimmon leaves and white paper in musk, angelica, cornus oil, antler extract It is characterized by technical composition.

For the musk, angelica, cornus oil, and antler extracts, each material is finely pulverized to a size of less than 400 mesh, ethanol is added to the pulverized material, immersed for 1 to 10 days to extract, and then filtered to recover the extract, ratary What is obtained by distillation under reduced pressure using an evaporator is characterized by the technical composition.

The mixed powder of buckwheat, mung beans, red beans, yulpi, brown rice, persimmon leaves, and white paper is characterized in that each material is finely pulverized, powdered, and mixed at the same weight ratio.

The mixing ratio of the musk, angelica, cornus oil, and antler extract and the mixed powder of buckwheat, mung bean, red bean, yulpi, brown rice, persimmon leaves and white paper is a characteristic feature of the technology configuration.

A skin cosmetic composition for skin antioxidant and wrinkle improvement containing the natural complex extract is characterized in that it is applied to the skin in the form of a cream or mask pack formulation.

Skin anti-oxidation and wrinkle-improving skin cosmetic composition containing the natural complex extract of the present invention is a natural composition made by mixing the mixed powder of buckwheat, mung beans, red beans, yule blood, brown rice, persimmon leaves and white paper in musk, angelica, cornus milk, and antler extract. The complex extract has an anti-oxidation effect and has excellent wrinkle improvement effects.

In order to solve the above problems, the present invention is a skin antioxidant and wrinkle containing a natural complex extract formed by mixing mixed powders of buckwheat, mung beans, red beans, yulpi, brown rice, persimmon leaves and white paper in musk, angelica, cornus milk, antler extract The improvement skin cosmetic composition is characterized by the technical composition.

For the musk, angelica, cornus oil, and antler extracts, each material is finely pulverized to a size of less than 400 mesh, ethanol is added to the pulverized material, immersed for 1 to 10 days to extract, and then filtered to recover the extract, ratary What is obtained by distillation under reduced pressure using an evaporator is characterized by the technical composition.

The mixed powder of buckwheat, mung beans, red beans, yulpi, brown rice, persimmon leaves, and white paper is characterized in that each material is finely pulverized, powdered, and mixed at the same weight ratio.

The mixing ratio of the musk, angelica, cornus oil, and antler extract and the mixed powder of buckwheat, mung bean, red bean, yulpi, brown rice, persimmon leaves and white paper is a characteristic feature of the technology configuration.

A skin cosmetic composition for skin antioxidant and wrinkle improvement containing the natural complex extract is characterized in that it is applied to the skin in the form of a cream or mask pack formulation.

Hereinafter, it will be described in detail through embodiments of the present invention so that those of ordinary skill in the art can easily implement the present invention. However, the present invention may be implemented in various different forms, and is not limited to the embodiments and drawings described herein.

First, the skin cosmetic composition for skin antioxidant and wrinkle improvement containing the natural complex extract of the present invention is prepared by mixing the mixed powder of buckwheat, mung beans, red beans, yule blood, brown rice, persimmon leaves and white paper in musk, angelica, cornus milk, and antler extract. Is formed.

Deer antler (Cervi Parvum Cornu) is a deer belonging to the genus Cervidae, C. neippon Temminck, C. elaphus.L, or C. canadensis Erxleben. An adult horn that has not been ossified or slightly ossified is cut and dried, and has been traditionally used as a drug in oriental medicine. In addition, the main constituents of antlers are lipids, proteins, carbohydrates, minerals and trace elements, and it is known that various physiological activities are expressed due to these components, and antlers are best known for their tonic effect, It is known to be effective in treating symptoms such as numbness and weakness in the back and knees. Research on the physiological function of antlers has been actively conducted in the field of oriental medicine in Korea, with anti-fatigue effect, increasing immune activity, promoting protein synthesis, enhancing endocrine function, hematopoietic function, increasing immune activity, analgesic effect, and lowering cholesterol. , Liver function improvement, growth promotion and diabetes treatment effects have been reported (Park PJ, et al, 2005).

Cornus officinalis refers to the fruit of a cornus tree, a deciduous tree of the Cornaceae family, the seeds are picked and dried in the sun. Cornus milk, as a characteristic of oriental medicine, tastes sour, is slightly warm in nature, is good for liver and nerves, has a diuretic effect, lowers blood pressure, helps digestion of proteins, and has been reported to have anticancer and antibacterial effects (Seo KI, et al. al, 1999) In addition, the reported components of cornus milk include gallic acid, malic acid, tartaric acid, ursolic acid, morronoside, and loganine. There are iridoid glycosides such as (loganin) and sweroside, and the isolation of tannins has also been reported (Park EB, et al, 2012). Various functional studies such as antioxidant research, anti-cancer substance separation, antibacterial activity separation, and nutrient analysis have been reported on cornus milk (Lee KY, et al, 2009).

Angelica gigas Nakai (Angelica gigas Nakai) is the roots of perennial vegetation in the family parsley family and dried before flowering. Angelica is known to have a blood-supplementing effect that replenishes blood, and is known to be prescribed as a blood-blood for anemia and women's diseases. The main component that exhibits the pharmacological action of Angelica is a substance called decursin, a derivative of coumarin, in addition to decursinol, umbelliferon, β-sitosterol, etc. Contains. Coumarine derivatives are known to be used in acute myocardial infarction as an anticoagulant used in hemostasis to prevent bleeding in the past. It was found that decursine, the main component of Angelicae, exhibits anticancer activity and has the effect of enhancing the activity of protein kinase C. The fractions of Angelicae are human immune cells such as B cells and T cells, and natural killer cells. It has been reported to promote the growth of (NK cells) (Kim JH, et al, 2006).

Musk is a dry musk sac (pod) secretion of a male musk roe deer. The active ingredient is muscone. In addition, cholesterol, calcium phosphate, ammonium carbonate, Contains fat, minerals, fiber, etc., and has a strong heart action, anti-inflammatory action, β-adrenaline effect enhancing action, capillary permeability hyperinhibition action, anti-thrombin action, etc. Excitement, heart feeling, tonicity, soothing , It has been used as an antidote for antispasmodics and drainage (Choi, YJ, et al, 1996).

The four medicinal herbs of deer antler, cornflower, angelica and musk are known as Gongjindan, and Gongjindan prevents aging and disease, when the energy is weakened by surgery or a major illness, and the energy is reduced due to mental and physical overwork. In case of severe chronic fatigue, recovery from rest is slow, congenital weakness, low blood circulation, and heavy and numbness, nervousness and dizziness and insomnia, excessive stress and alcohol It is used in weakened cases and is also effective in alleviating skin aging, and is also known to improve skin wrinkles and have a skin whitening effect.

In the present invention, the musk, angelica, cornus oil, and antler extract are finely pulverized to a size of less than 400 mesh, and then ethanol is added to the pulverized material, immersed for 1 to 10 days, and extracted, and then filtered to recover the extract. And, use the one obtained by distillation under reduced pressure using a ratary evaporator.

On the other hand, the buckwheat used in the present invention is the scientific name Fagopyrum esculentum Moench, and generally, when the flowers begin to bloom, the upper branch with leaves and flowers is cut and dried as it is and used as a pharmaceutical raw material. The main ingredient of buckwheat is rutin, and its content varies depending on the growing period, plant part, and method of drying the herbs. The content of rutin is highest after 25 to 30 days from the time the seeds are planted and the buds sprout, and the highest is just before the flowers bloom. The content of rutin per part is 45% for buds, 14% for blooming flowers, 9-10% for leaves, and 03-04% for stems. It has the effect of lowering the permeability of capillaries and restoring fragility by the action of vitamin P in rutin. The mechanism of action is not clear, but it is believed to increase vascular resistance by inhibiting the oxidation of adrenaline and ascorbic acid, thereby increasing the amount of accumulation in the body. In Dongeui-chil, buckwheat rice was used for the purpose of redness, swelling, anti-inflammatory, poisonous fever for children, etc. In the private sector, buckwheat flour paste and buckwheat porridge are made and eaten when suffering from high blood pressure or hepatitis, and rutin is used as a pill. It is known to be effective in preventing capillary bleeding, cerebral bleeding and hardening of arteries. In particular, buckwheat is effective for skin troubles in which sebum, dead skin cells, and waste products are accumulated, and acts so that the pores breathe by cleanly controlling skin impurities.

Also, Mung bean (Phaseolus radiatus, Mung bean), also known as Andu or Gildu, is a seed of mung bean, an annual plant of the legume family. It grows well on loam in a warm climate (a black soil suitably mixed with sand and clay), and its height is 30. It is ∼80cm. The stem is thin, has vertical veins, 10 nodes, and branches. Leaves come out with a pair of cotyledons and freshly formed leaves, and then 3 small compound leaves. Unlike other legumes in the legume family, these mung beans have been used as a treatment for skin diseases, antipyretic, and detoxification in oriental medicine. Until recently, mung beans are used for facial massage in the form of dry powder or mixed with water or honey, or as foods such as mung bean pancake made by mixing mung bean powder with water and green pomuk made by extracting starch from mung bean, bean rice cake, mung bean porridge, and bean sprouts. It has been used since ancient times. In the present invention, it is preferable to use mung bean seeds (fruits). In particular, the protein contained in mung beans is superior to other cereals in removing sebum. Since it is widely used as a skin care material, it has no side effects and can be used with confidence even for sensitive skin.

Red beans have long been known as foods to repel diseases and ghosts. On the winter day, eating red bean porridge was also to ward off diseases and ghosts through red beans. Red beans are also called sodu (小豆) and jeoksodu (赤小豆). The origin of red beans is not clear, but the origin is estimated to be in China, and it is a crop cultivated in the East for a long time. It is thought to have come from China in Korea and has a long history of cultivation. The stem is somewhat thinner and longer than the beans, but tends to be vines and is prone to fall. The height is usually 50~90cm, and the stem is green or reddish purple. The leaves are alternate, and it is a compound leaf consisting of 3 small leaves, and there is a small stipule at the bottom of the long petiole. Small leaves are egg-shaped or rhombic-shaped. In summer, long peduncles emerge from the leaf axils and 4-6 yellow phenotypes hang. The pod is thin and long cylindrical, about 10cm long, without hair. When mature, it becomes light yellow, light brown, and black brown, and contains 3 to 10 seeds. Seeds are 4-8mm long and 3-7mm wide, cylindrical, and both ends are round. Color is so-called red bean, other white, light yellow, black, light

There are dark green and black patterns. Red beans are classified into ordinary red beans with straight stems and vine red beans. According to ecological characteristics, red beans are classified into summer red beans and autumn red beans, and red red beans, black red beans, blue red red beans stained according to the color of the seed shell. Red beans are warm in nature, sweet in taste, and not poisonous. Red beans contain 214g of protein, 06g of lipid, 566g of sugar, 32g of ash, 37g of fiber, and a large amount of vitamin B1 per 100g of red bean. Red beans are very nutritious and contain saponin that protects the skin, so they are good for atopic dermatitis and spots and freckles, and help prevent and treat beriberi disease. Boiled red beans cure nephritis, and folk remedies for boiling red beans, kelp, and pumpkin are known for diabetes. Red beans are good for preventing adult diseases after their 40s, but old ancestors showed wisdom to eat red beans on every new and full moon every month to protect their health. In the old literature, many benefits of red bean are mentioned. In "Byeolrok of Myung", it is said that red beans are good for sogal, and it is good for sogal. In "Yakseong Herb", it is written that red beans control heat poisoning, eliminate evil blood, and strengthen rain and stomach. Red beans are beneficial to urine, soothes dropsy, removes inflammation, and has various effects to release alcohol. In particular, red beans contain a lot of vitamin B1, and when vitamin B1 is insufficient in our body, various symptoms such as beriberi disease, nerves, stomach, and heart appear. In particular, vitamin B1 is deeply related to nerves, and when it is insufficient, symptoms such as loss of appetite, fatigue, sleep disorders, memory loss, and nervous breakdown appear. Therefore, red beans are a very good food for the prevention and combat of these symptoms and diseases, and especially for mental workers and students who pay a lot of attention.

Chestnut Inner Bark means the inner bark of chestnut fruit, and chestnut tree has been used not only as fruit but also raw leaves, chestnut flowers, chestnut bark, chestnut bark and inner skin. Chestnut bark contains 9-10% tannins, and leaves 9-10% tannins, vitamin K 008%, and tritertenoids such as flavonoid glycosides hyperin, ursolic acid, betulin and lupeol. Tannins are usually contained in the roots and bark, but can also be found in leaves, and tannins coagulate proteins in the skin or mucous membranes and turn them into substances that are resistant and insoluble. These properties of tannins are useful when bacteria and fungi invade, because tannins deprive the substrate of microorganisms and make the skin more resistant. Therefore, Yulpi quickly and soothes skin diseases related to inflammation, eczema and itching, and has an effect on excessive sweat secretion and diarrhea.

Brown rice is rice that has been dried and threshed, and then the rice hull is peeled off. Brown rice is divided into rice bran, rice bran, and endosperm. Rice bran is rich in fat, protein, essential fatty acids, essential amino acids, vitamins, dietary fiber and minerals. The vitamins in rice bran also contain B vitamins such as myoinositol, choline and niacin, and tocopherol, an antioxidant that prevents cell aging and damage. As minerals, phosphorus, potassium, magnesium, and calcium are contained. Also, rice bran contains proteins, cytosterol, and dietary fiber, which are known to have an effect of lowering blood cholesterol, and gamma oryzanol components promote blood circulation and skin metabolism. It is also known to have whitening and UV blocking effects. Rice, which accounts for most of the nutrients of brown rice, contains a large amount of calcium, magnesium, potassium, and iron in addition to essential amino acids, tocopherol (vitamin E), vitamins B1, B2, and B6. Vitamin E improves blood circulation in peripheral blood vessels, transports oxygen and nutrients to cells, and releases waste products and carbon dioxide, and plays a role in inhibiting the increase of lipid peroxide, which prevents the oxidation of fat in the body and weakens cell movement.

Persimmon leaf is the leaf of the persimmon tree (Diospysos kaki). Persimmon tree is a fruit tree peculiar to East Asia and is native to Korea, China, and Japan. 100g of persimmon leaves contain a lot of vitamin C, about 200mg, 2 to 3 times more than mandarin oranges. Vitamin C of persimmon leaf extract is relatively safe and highly effective because it is resistant to heat. It has no toxicity and side effects, has a diuretic effect, stabilizes blood pressure, and is recognized for its excellent efficacy in kidney disease and heart disease. In addition, there are many carotenes that show the effect of vitamin A, improving resistance to diseases, excellent efficacy in preventing adult diseases, preventing hangovers and relieving hangovers, hemostasis, stopping diarrhea and stomach upset, anti-aging effects, skin beauty effects, colds. Prevention, good for brain cell health, good for pregnant women and menstruation. The tannins contained in persimmon leaves remove swelling from edema and detoxify toxic substances such as lead, arsenic, and mercury.

Baekji (Angelica dahurica root) is a herbal name that refers to the root portion of the umbel (Angelica dahurica Bentham et Hooker). The roots are pale yellowish brown, but they are enlarged, and when they are bolstered, they become woody and die. As a herbal medicine, 1~2 years old is good, but the flower stalk is not raised. It is a pain reliever that relieves headache, stuffy nose, and runny nose caused by a flu, and is effective for gastrointestinal disorders, prenatal laryngeal headache, dizziness, toothache, facial nerve pain, and paralysis. In addition, it is known that it is effective in bleeding after menstruation, when blood is mixed with lobster, stool, headache, swelling, swelling, and skin ulcers caused by sinusitis.

In the present invention, the mixed powder of buckwheat, mung bean, red bean, yulpi, brown rice, persimmon leaf, and white paper is a powder obtained by pulverizing each material finely and mixing them in the same weight ratio, and the natural composite of the present invention Skin anti-oxidation and wrinkle-improving skin cosmetic composition containing the extract is composed by mixing the musk, angelica, cornus milk, antler extract and the mixed powder of the buckwheat, mung bean, red bean, yulpi, brown rice, persimmon leaves and white paper in a 1:1 weight ratio do.

It is preferable that the skin cosmetic composition for skin antioxidant and wrinkle improvement containing the natural complex extract of the present invention is applied to the skin in the form of a cream or mask pack formulation.

[Preparation of skin cosmetic composition for skin antioxidant and wrinkle improvement containing the natural complex extract of the present invention]

After pulverizing 20 g of musk, 100 g of angelica, 100 g of cornflower, and 100 g of deer antler into pieces of less than 300 mesh each, 1 liter of ethanol was added to the pulverized material, immersed for 5 days for extraction, and then filtered to recover the extract, ratary The extract was prepared by distillation under reduced pressure using an evaporator.

Next, buckwheat, mung beans, red beans, yulpi, brown rice, persimmon leaves, and white paper are each powdered to prepare a mixed powder by weighing 30 g, and the mixed powder is mixed with the extract at the same weight ratio of 1:1 to make a thin dough (cream) It was prepared in the form.

[Measurement of Antioxidant Effect of Skin Antioxidant and Anti-Wrinkle Skin Cosmetic Composition Containing Natural Complex Extract of the Invention]

In order to measure the antioxidant effect of the skin cosmetic composition of the present invention prepared in [Example 1], a Free Radical Scavenging Activity Test was conducted. Specifically, 3 tubes containing 1 ml of a 02mM DPPH (1,1-diphenyl-2-picryhydrazyl, Sigma) solution were prepared. One tube was added with purified water as a control group, and the other two tubes were each added with Pseudomonas mushroom extract and the skin cosmetic composition of the present invention as an experimental group. Then, after each of the three tubes was allowed to stand at room temperature for 10 minutes, the absorbance was measured at 525 nm for the three tubes, and the free radical erasure rate of the experimental group was calculated using the equation. The results are shown in the following [Table 1].

Free radical scavenging rate (%)=[100-{(absorbance of experimental group-absorbance of control group)/absorbance of control group}×100]

Experimental group Free radical scavenging rate (%) Psoriasis mushroom extract 22.4 Skin cosmetic composition of the present invention 38.4

As shown in [Table 1], since the cosmetic composition of the present invention exhibits a free radical scavenging rate of about twice or more than that of Pseudomonas mushroom extract, from this, the skin cosmetic composition of the present invention has much better antioxidant effect than Pseudomonas mushroom extract. Can be seen.

[Measurement of the effect of increasing fibroblast collagen biosynthesis of skin cosmetic composition for skin antioxidant and wrinkle improvement containing the natural complex extract of the present invention]

A 96-well plate containing 10% fetal bovine serum in DMEM (Dulbecco's modified Eagle's media) was prepared. Human fibroblasts were inoculated on the plate at a concentration of 5,000 cells/well, and cultured under conditions of 37° C. and 5% CO 2 until they grew about 70% to 80%. Subsequently, the skin cosmetic composition of the present invention prepared in [Example 1] and the mushroom extract were added to the plate as an experimental group, and cultured for 1 day, respectively. Each of the cultured cell culture media was collected and the degree of collagen production was measured using a commercially available collagen biosynthesis analyzer (Japan, Takara Shuzo Co, Ltd Catalog #: MK101).

Specifically, the collected cell culture solution was added to a 96-well plate uniformly coated with the primary collagen antibody, and an antigen-antibody reaction was performed in a constant temperature bath for 3 hours. Then, the secondary collagen antibody to which the chromophore was bound was added to a 96-well plate and reacted for 15 minutes. Then, a color developing substance was added to induce color development at room temperature for 15 minutes. Then, 1M sulfuric acid was added to stop the color development. The 96-well plate in which color development was stopped was measured for absorbance at 450 nm using an absorbance meter, and the degree of collagen production was calculated according to the following equation. The results are shown in the following [Table 2]. The absorbance of the control group was measured using a plate to which nothing was added.

Collagen production rate (%) = (reaction absorbance of the experimental group/reaction absorbance of the control group) × 100

Experimental group Collagen production rate (%) Psoriasis mushroom extract 20.5 Skin cosmetic composition of the present invention 60.4

As shown in [Table 2], it can be seen that the skin cosmetic composition of the present invention has a much better collagen production rate than that of the Pseudomonas mushroom extract, so that the collagen biosynthesis effect is much better.

The above description is merely illustrative of the technical idea of the present invention, and those of ordinary skill in the art to which the present invention pertains will be able to make various modifications and variations without departing from the essential characteristics of the present invention. Accordingly, the embodiments disclosed in the present invention are not intended to limit the technical idea of the present invention, but to explain the technical idea, and the scope of the technical idea of the present invention is not limited by these embodiments. The scope of protection of the present invention should be interpreted by the following claims, and all technical ideas within the scope equivalent thereto should be interpreted as being included in the scope of the present invention.

Claims (5)

Skin cosmetic composition for skin antioxidant and wrinkle improvement containing natural complex extracts, characterized in that it is formulated by mixing mixed powders of buckwheat, mung bean, red bean, yulpi, brown rice, persimmon leaves and white paper in extracts of musk, angelica, cornus milk, and antler
The method of claim 1,
For the musk, angelica, cornus oil, and antler extracts, each material is finely pulverized to a size of less than 400 mesh, ethanol is added to the pulverized material, immersed for 1 to 10 days to extract, and then filtered to recover the extract, ratary Skin cosmetic composition for skin antioxidant and wrinkle improvement containing natural complex extracts obtained by distillation under reduced pressure using an evaporator
The method of claim 1,
The mixed powder of buckwheat, mung bean, red bean, yulpi, brown rice, persimmon leaf and white paper is a powder obtained by pulverizing each material finely and then mixing them in the same weight ratio. Skin antioxidant and wrinkles containing natural complex extracts Skin cosmetic composition for improvement
The method of claim 1,
Skin antioxidant containing natural complex extract, characterized in that the mixing ratio of the musk, angelica, cornus, antler extract and the mixed powder of the buckwheat, mung bean, red bean, yulpi, brown rice, persimmon leaves and white paper is mixed in a ratio of 1:1 by weight, and Skin cosmetic composition for wrinkle improvement
The method according to any one of claims 1 to 4,
Skin antioxidant and wrinkle-improving skin cosmetic composition containing the natural complex extract is applied to the skin in the form of a cream or mask pack formulation, and a skin cosmetic composition for skin antioxidant and wrinkle improvement containing a natural complex extract