KR101712559B1 - A cosmetic composition containing ceriporia lacerata extract for skin-whitening and skin-antiwinkle and cosmetics container including the same - Google Patents

Abstract

The present invention relates to a cosmetic composition by mixing a proper amount of a Ceriporia lacerata extract, a spirulina extract and a Sparassis crispa Wulf.:Fr. extract, or adding a certain amount of a natural extract having whitening activity, wrinkle reducing activity, and whitening and wrinkle reducing activity, and to cosmetics produced by adding the cosmetic composition at the certain ratio with respect to the total weight of the cosmetics.

Description

TECHNICAL FIELD The present invention relates to a cosmetic composition for skin whitening and wrinkle improvement containing a saponin extract, a seripolylacerate extract, and a cosmetic composition using the same. [0002]

The present invention relates to a cosmetic composition comprising a proper amount of the Seripolya cerasita extract, Spirulina extract,

Or a natural extract having a whitening activity, a wrinkle improving activity, a whitening effect and a wrinkle improving activity, in a certain ratio, to form a cosmetic composition,

And a cosmetic composition prepared by adding the cosmetic composition at a predetermined ratio to the total weight of the cosmetic.

Regarding the cosmetic composition for skin whitening and wrinkle improvement containing the Seripolia cerasita extract of the present invention and cosmetics using the same, Korean Patent Registration No. 10-0848515 (registered on July 21, 2008) A method of extracting wild mushroom, green tea, clove, and Youngsil extract, and a method of producing whitening cosmetics using the mixed extract thereof, "published in Korea, published on Feb. 26, 2004, published by Korea Patent Application No. 10-2004-0043971 Patent No. 10-2014-0114240 (Publication date 2014.09.26) 'Cosmetic composition for skin whitening', Korean Patent Laid-Open No. 10-2014-0114239 (Publication date 2014.09.26) 'Whitening cosmetic composition', Korean Patent No. 10-1251040 WO 01/32013 (published on Oct. 28, 2004) " Containing Isoflavone separated from natural materials " A skin whitening composition containing an extract and a skin whitening cosmetic comprising the composition are disclosed.

The present invention relates to a cosmetic composition comprising a mixture of Seripolya cerasata extract, Spirulina extract,

Or a natural extract having a whitening activity, a wrinkle improving activity, a whitening activity and a wrinkle reducing activity in a certain ratio to provide a cosmetic composition, which has a whitening activity and a wrinkle reducing activity, It has not been disclosed.

Korean Patent No. 10-0848515 (registered on July 21, 2008) Korean Patent Laid-Open No. 10-2004-0043971 (Publication date 2004.05.27) Korean Patent Publication No. 10-2014-0114240 (Publication date 2014.09.26) Korean Patent Publication No. 10-2014-0114239 (Publication date 2014.09.26) Korean Registered Patent No. 10-1251040 (Registered on March 29, 2013) Korean Patent Publication No. 10-2004-0091178 (published date Oct. 28, 2004)

It is an object of the present invention to provide a cosmetic composition having a whitening effect and a wrinkle-reducing effect, which are composed of a mixture of Seripolya cerasita extract, Spirulina extract, and Mulberry extract.

It is another object of the present invention to provide a cosmetic composition which is further comprised of a natural extract having whitening activity, wrinkle reducing activity, whitening activity and wrinkle improving activity at the same time.

It is another object of the present invention to provide a cosmetic product prepared by adding the cosmetic composition at a predetermined ratio to the total weight of the cosmetic product.

In order to achieve the above object,

The present invention relates to a method for producing a mycelium of the present invention comprising drying a mycelial culture liquid of a seripolylacerate by a freeze drier, pulverizing the lyophilized seripioria cererata using a crusher and then pulverizing the powder at 80 to 120 mesh sieve at -15 ° C to -25 ° C The mixture was added to 80% methanol (MeOH) at a ratio of 1:20 to 30, and the mixture was extracted at room temperature for 3 to 5 days. The extracted extract was firstly filtered to obtain 3000 × Centrifugation is carried out at a gravity acceleration (g) for 2 to 4 minutes, 0.01 to 70% by weight of a Seripolya cerasata extract having only a supernatant,

Spirulina was mixed with distilled water having a weight of 30 to 50 times that of Spirulina and then shaken in an incubator at 20 to 27 ° C and 100 to 200 rpm for 65 to 80 hours and the shaken solution was stirred for 15 to 30 minutes using an ultrasonic disperser processor, and the dispersed solution is separated from the supernatant and the precipitate in a centrifuge at 6.000 to 9,000 rpm for 10 to 30 minutes, 0.01 to 70 wt% of spirulina extract obtained by taking the supernatant,

Dried pine mushroom was pulverized to 80 to 120 meshes using a pulverizer, and the granular mushroom powder and distilled water having a weight of 10 to 30 times the weight of the granular mushroom powder were mixed and then the pH was adjusted to 6.0 to 10.0 , And a mixture of 0.01 to 70 wt% of a mushroom extract of P. japonicus extracted at 100 to 140 rpm at 90 to 100 ° C for 5 to 20 hours. The skin whitening, wrinkle improvement, The present invention provides a cosmetic composition.

The present invention provides a cosmetic having high functionality by adding the cosmetic composition at 0.1 to 50 wt% based on the total weight of the cosmetic.

The cosmetic composition according to the present invention is excellent in whitening effect and wrinkle-reducing effect by being composed of a natural material extract having excellent antioxidant, whitening effect, anti-aging function and moisturizing effect in addition to the saponin extract.

A high-performance cosmetic having whitening activity and wrinkle-reducing activity can be provided by adding such a high-function cosmetic composition to cosmetics at a predetermined ratio.

Hereinafter, a detailed description of the above-described technical configuration will be given.

As described above,

The cosmetic composition for treating skin whitening and wrinkles containing the Seripolyaracerata extract according to the present invention is characterized in that the mycelial culture liquid of the seripolylacerate is dried by a freeze drier and the freeze-dried Seripolyaracerata is pulverized The powder was cooled at -15 ° C to -25 ° C, and the seripolylaceratata powder, which was stored frozen, was added to 80% methanol (MeOH) at a ratio of 1:20 to 30 by weight After extracting at room temperature for 3 to 5 days, the extracted extract is firstly filtered and centrifuged at 3000 × gravity acceleration (g) for 2 to 4 minutes. 0.01 to 70 wt% of Seripolia cerasata extract,

Spirulina was mixed with distilled water having a weight of 30 to 50 times that of Spirulina and then shaken in an incubator at 20 to 27 ° C and 100 to 200 rpm for 65 to 80 hours and the shaken solution was stirred for 15 to 30 minutes using an ultrasonic disperser processor, and the dispersed solution is separated from the supernatant and the precipitate in a centrifuge at 6.000 to 9,000 rpm for 10 to 30 minutes, 0.01 to 70 wt% of spirulina extract obtained by taking the supernatant,

Dried pine mushroom was pulverized to 80 to 120 meshes using a pulverizer, and the granular mushroom powder and distilled water having a weight of 10 to 30 times the weight of the granular mushroom powder were mixed and then the pH was adjusted to 6.0 to 10.0 , And the mixture is then blended with a mixture of 0.01 to 70% by weight of an extract of Mushroom mushroom extracted at 90 to 100 DEG C for 100 to 140 rpm for 5 to 20 hours.

The cosmetic composition is added in an amount of 0.1 to 50 wt% with respect to the total weight of the cosmetic, thereby producing a cosmetic having whitening activity and wrinkle-reducing activity.

It has been reported that the mechanism by which active oxygen generated by ultraviolet rays, which is a main cause of skin pigmentation, promotes skin pigment formation, and that these active oxygen scavenging effects are effective in inhibiting melanin pigment formation. Thus, the antioxidants not only inhibited the formation of melanin pigment produced by the continuous oxidation process but also recognized as a whitening substance which can prevent formation of melanin pigment even by scavenging the active oxygen.

Creation of wrinkles occurs as the skin becomes thinner, the moisture content of the stratum corneum decreases, and the skin becomes dry and stained due to the reduction of the elastic fibers, elastin and collagen in the dermis. Therefore, in order to improve wrinkles and prevent aging, it is necessary to have an increase in moisturizing power, promotion of collagen synthesis, and antioxidant function.

The seripolylacerate has been used as natural bioremediation due to its lignin-decomposing action, and has a whitening effect, an antioxidative activity, a skin irritation degree, a moisturizing effect, a skin elasticity, and a skin regeneration function.

When the content of the Seripolya cerasita extract having the above characteristics is less than 0.01 wt%, the whitening effect and the wrinkle reducing effect are inferior. When the content exceeds 80 wt%, the production cost increases. It is preferable that the amount of the Seripolya cerasata extract is limited within a range of 0.01 to 70 wt% with respect to the total weight of the cosmetic composition.

Spirulina is one of the oldest algae on the planet. Its color is cyan and its shape is long and spiral.

Spirulina extract is ideal for skin because it has a very ideal composition such as fatty acid composition, minerals and beta-carotene as compared with chlorella with similar composition, thin wall of cells and mainly composed of peptide glycans and very high absorption rate of skin.

Spirulina contains high antioxidants and vitamins A, C and E. About 65% of the whole ingredients are pure protein, and 50 essential nutritional ingredients including protein, phosphorus, fat, vitamins and minerals which are the five essential nutrients are included. Pycocyanin and strong natural It contains SOD, an antioxidant, which is known to improve skin health, antioxidant activity, and improve blood cholesterol.

When the amount of the spirulina extract is less than 0.01 wt%, the effect of improving wrinkles is deteriorated. When the amount of the spirulina extract is more than 75 wt%, the degree of improvement of the wrinkle improving effect is insignificant. To 70% by weight.

The Sparassis crispa is a mushroom belonging to the Aphyllophorales mushroom. It is a mushroom with incense like matsutake mushroom. It grows mainly on the ground and stubble near the roots of coniferous trees such as pine, hemp, and pine.

The mushroom has a role of removing active oxygen which is revealed as a main cause of skin aging by antioxidant activity.

When the amount of the extract of Zanthoxylum mushroom is less than 0.01 wt%, the whitening effect is lowered. When the amount of the extract is more than 70 wt%, the degree of improvement of the whitening effect is insignificant, By weight based on the total weight of the composition.

In addition to the basic constitution of the above-mentioned cosmetic composition, in the present invention, any one or two or more kinds of whitening active extracts selected from the group consisting of red sea bream extract, Kwonwoowoo extract, Saururus chinensis extract, wt%,

The present invention relates to an extract for improving wrinkle-improving activity, which comprises a mixture of 0.1 to 99.9 wt% of one or more wrinkle-improving active extracts selected from Lycium chinense extract, Inca extract, Blueberry extract, % Of the mixed extract may be further added in an amount of 0.01 to 70 wt%.

The present invention also provides a mixed extract obtained by mixing 0.1 to 50 wt% of root root extract, 0.1 to 50 wt% of caspase extract, 0.1 to 50 wt% of ganoderma extract and 0.1 to 50 wt% of crude extract, which can simultaneously impart whitening activity and wrinkle- Can be added.

The extract for the whitening activity is preferably prepared by mixing 0.1 to 50 wt% of an extract of Seaweed extract, 0.1 to 50 wt% of a crude extract, 0.1 to 50 wt% of a Saururus chinense extract, and 0.1 to 50 wt% of a Chrysanthemum orientalis extract use.

In order to further enhance the function of the extract, the extract for wrinkle-improving activity is composed of a mixture of 0.1 to 50 wt% of the extract, 0.1 to 50 wt% of the extract of Inca extract, 0.1 to 50 wt% of the extract of blueberry, and 0.1 to 50 wt% do.

Hereinafter, components of the whitening active extract will be described.

Polygonum multiflorum Thunb. (Polygonum multiflorum Thunb.) Is a root of perennial grass belonging to Polygonaceae. It is also called Sasao, Red mooring, Sueongdong, Suo, and Stem is called Haji.

In the present study, it was reported that the phenolic compounds, flavanoids, anthraquinones, atilbenes, and tannins were reported to be separated and reported have. The major components are 2,3,5,4'-tetrahydroxystilbene-2-beta-D-glucoside, rhein, emodin, aloe-emodin, chrysophanol, physcion and their glycosides.

As a specific example of the above-described extraction of sodium hypochlorite, 250 g of dried sodium hypochlorite is pulverized, 1 L of methanol is added thereto, and the mixture is allowed to stand at room temperature for 72 hours. The extract is filtered twice using 150 mm filter paper (Whatman, UK). The filtered extract is evaporated to dryness in a constant temperature water bath at 40 ° C using a rotary evaporator (EYELA, Japan), and the extract is concentrated and freeze-dried.

Geumgwoo (紫金 牛) is the underside of the Ardisia japonica Blume, which is the evergreen bush of Myrisinacae.

Ardisia japonica Blume is a relatively effective jade gangdam effect in one room. It is especially effective in relieving wasted heat (pulmonary fever), respiratory stimuli, dandruff in the case of many sputum, and controlling jaundice caused by moist heat , Diuretic action, acute and chronic nephritis, face and eye swelling gives off symptoms.

Quercitin, bergening, quercitrin, triterphene compounds, tannin, myricitrin, and ilexol have been found to be the main constituents of Kwangwoo woo.

As a concrete example of the extraction, the water is used as a solvent for extraction, and the extract is extracted by adding 30-fold water to 100 g of the pine needle and then immersing at room temperature for 2 to 3 weeks. After treating with hot water at 95 ° C for 2 hours, immersion extraction is carried out. The extract was concentrated under reduced pressure at 40 ° C to 70 ° C and freeze-dried. The extract should be stored at -80 ° C sealed.

Saururus chinensis Baill. Saururus chinensis Baill. Is a perennial herbaceous plant belonging to Saururus chinensis. It is called Saururus chinensis because it blooms in July ~ August and has 3 ~ 4 white leaves.

Saururus chinensis has long been used for the treatment of detoxification, hepatitis, hepatitis, and jaundice. Saururus chinensis has been known to reduce the incidence of heart-related diseases and lower hypertension due to the clearing of blood and blood vessels. Quercetin, quercetrin, isoquercitrin, rutin, lignan and water soluble tannin are known to be contained in Saururus chinensis. Quercetin and quercetrin are known as flavonoids.

Quercetin, a major component of Saururus chinensis, has been shown to have antimicrobial and antioxidant effects such as antioxidant activity, antimicrobial activity, capillary strengthening activity, and reduction of the activity of carcinogenic substances. Antihypertensive and anti-inflammatory effects are known

As a specific example of the Saururus chinensis extract, 200 g of Saururus chinensis leaf is placed in 2,500 ml of 100% ethanol, extracted at room temperature for 72 hours, filtered with gauze, and then the extract is concentrated under reduced pressure at 50 ° C and freeze-dried.

Angelica gigas Nakai is Angelicae Gigantis Radix and its scientific name is Angelica gigantis Nakai. It is a perennial herbaceous plant of Apiaceae which is Angiospermae.

Chrysanthemum morifolium contains coumarine derivatives such as Decursin, Decusinol and Nodakenin and derivatives of α-pinene, Limonene, β-eudesmol, , Elemol, and other essential oils.

As a specific example of the above-mentioned true Angelica keiskei koidzu extract, 5 g of distilled water is added to 300 g of Angelica gigas Nakai, and the mixture is extracted from room temperature to 90 ° C. The extract is filtered and concentrated under reduced pressure in a bath of 40 ° C or lower using a rotary evaporator, .

And more preferably 0.1 to 50% by weight of the extract of Sasa extract, 0.1 to 50% by weight of extracts of Kwangwoo woo, 0.1 to 50% by weight of Saururus chinense extract, 0.1 to 50% % Of the total weight of the composition.

Such a blending ratio composition is intended to enhance the whitening effect through optimized blending composition between the extracts, and it is preferable to form the blending ingredient according to the blending ratio shown above.

Hereinafter, components constituting the wrinkle-improving active extract will be described.

Lycii Cortex Radicis (LCR) is known as the root bark of Gugija, and its gingiva has a unique flavor. Betaine, β-sitosterol glucoside, linoleic acid, scopoletin and kukoamine have been reported

As a specific example of the extraction of the above-mentioned cornucopia, 200 g of Lycium chinense was added to a round flask with 10-fold (w / v) amount of distilled water, followed by heating at 100 ° C. for 4 hours, followed by filtration with gauze, It is concentrated at 80 ° C, cooled at -80 ° C for a day, lyophilized at -40 ° C and used.

Geranium nepalense (Geranium nepalense) is a generic term for geranium thumbergii, also known as the gibbous sprout, and the upper part of the flowering plant. In oriental medicine and civilian medicine, it is used as a medicine for phlegm, constipation, constipation, large intestine, cystitis, skin disease, gastric ulcer and the like. Its main ingredient is known as tannin and gives a pungent taste.

As a specific example of the extract of Geranium sp., Geranium thunbergii (dry weight: 460 g) is extracted three times with 3 L of MeOH as an extraction solvent at 80 ° C for 3 hours with shaking to obtain an extract. The extract is concentrated under reduced pressure and lyophilized before use.

Vaccinium uliginosum L. is a deciduous tree belonging to the genus Ericaceae. It is a wild plant which mainly grows in a large area in a high mountain area. Studies on Bilberry (Vaccinium myrtyillus) and Blueberry (Vaccinium corymbosum or Vaccinium angustifolium) which are close to systematic blueberry have been carried out, but studies on the components of blueberry fruit are rare. Blueberries have been widely used as folk remedies because of their good blood circulation, diuretic action and arthritis, and it is known that they contain a large amount of Flavonoid which is an important physiologically active substance.

As a specific example of the blueberry extraction, 200 g of freeze-dried blueberry fruit is extracted with 2,000 mL of an extraction solvent (distilled water), and then the extraction solvent is removed and dried to obtain an extract. It is refluxed three times for 6 hours while shaking at 100 ℃ using distilled water (D.W.) and lyophilized.

The motherwort (Leonurus sibiricus Linne) is a biennial herb belonging to Lamiaceae (Labiatae) and is characterized by a square stem.

As a specific example of the motherwort extraction, 2 kg of dried motherwort is cut, followed by hot water extraction three times with MeOH in a water bath, followed by filtration and concentration under reduced pressure to obtain a MeOH extract. It is used by freeze drying.

More preferably, 0.1 to 50% by weight of the extracts of gingerol, 0.1 to 50% by weight of the extract of Inca, 0.1 to 50% by weight of blueberry extract and 0.1 to 50% by weight of extract of motherworm extract, Is preferably used.

Such a blending ratio is intended to enhance the wrinkle-improving effect through an optimized blending configuration between the extracts, and it is preferable to form a blending ratio according to the blending ratio given above.

The extract for wrinkle-improving activity is prepared at an optimal blending ratio of the components for improving the wrinkle-improving effect, and it is preferable to form the blending component according to the blending ratio given above.

Hereinafter, the constituents of the extract giving the whitening activity and the wrinkle-reducing effect at the same time will be described.

Lotus root has been known to be effective for women's beauty, such as moisturizing skin and eliminating acne and freckles. Lecithin in the lotus root prevents the deposition of cholesterol on the blood vessel wall and strengthens the blood vessel wall. It also helps to strengthen the blood vessel walls and to protect the nervous system, acetylcholine And it is known that the dementia prevention effect by the memory deceleration inhibiting action is also great. It is also reported that high potassium content is required for people with high blood pressure, contains mucin as a complex protein, and has cholesterol lowering action, gastric wall protection and detoxifying action. The main component of the rootstock is starch, but it contains a lot of amino acids such as quercetin, isoquercitrin, nelumboside, asparagine, azine, thyroxine and lecithin and pectin. Especially quercetin, isoquercitrin, nelumboside is a kind of polyphenols, which inhibits rancidity of fat in the body, softens hardened arteries, is effective in preventing arteriosclerosis, and has a strong antioxidant effect.

As a concrete example of extracting the lotus root, 200 ml of 100% ethanol (EtOH) is added to 20 g of a lotus root, and the mixture is refluxed for 3 hours with a cooler attached thereto. After filtration with filter paper (Whatman No. 2), the filtrate is concentrated under reduced pressure using a rotary vacuum evaporator and dried. The same test method as above is used for 80% and 70% ethanol solvent. Alternatively, 20 g of the lotus root is extracted by immersing 600 ml of 80% EtOH for one week. After filtration with filter paper (Whatman No. 2), the filtrate is concentrated under reduced pressure using a rotary vacuum evaporator and dried.

The casualties are the plants belonging to the mountain type and mainly distributed in Korea. Cnidium monieri (L). Cuss) or casualties (Torilisjaponica Decandolle). As a component, fruits contain various coumarin derivatives such as ostol, xantotoxin, isopimpinellin, bergapten and imperatorin.

As a specific example of the boss extraction, 1000 mL of 70% ethanol was added to 50 g of dried shreds, followed by shaking extraction at 100 RPM at 25 DEG C for 24 hours, filtration through a filter paper (whatman No. 2), and filtration of the filtrate using a rotary vacuum evaporator And concentrated using a concentrator (EYELH Co., Tokyo, Japan), lyophilized in a freeze dryer at -70 ° C, and stored frozen.

Giant (AngelicatenuissimaeRadix) is a perennial plant belonging to Umbelliferae, which blooms in late April and buds in the middle of April. The pharmacological effect of gobon is analgesic / antipyretic, and it is effective when it is pain caused by headache, back pain, and women's disease. 15-30% of the gonadal myrrh inhibitors have an inhibitory effect on many kinds of skin fungi commonly found in invitro. And it removes the wind and makes the new life to live well.

As a specific example of the extraction of the tumulus, 1000 mL of 70% ethanol was added to 50 g of dried gonad, and the mixture was extracted with shaking extractor at 100 RPM at 25 DEG C for 48 hours, filtered with filter paper (whatman No2), the filtrate was reduced in pressure using a rotary vacuum evaporator Concentrate in a concentrator (EYELH Co., Tokyo, Japan), freeze-dry at -70 ℃ in a freeze dryer, and store frozen.

Aconitum pseudolaevevarerectum is a perennial herb that grows in the shade of the forest. The major components of the genus were alkaloids such as avadharidine, lycoctonine and septentriodine, methyl-N- (3-carbamoylpro-pionyl) anthranilate and methyl-N- (2-acetamino-benzoyl) anthranilate And so on.

As a specific example of the above-mentioned real-life extractions, 1000 mL of 70% ethanol was added to 50 g of dried true bees, followed by shaking extraction at 100 RPM at 25 DEG C for 48 hours. The mixture was filtered through filter paper (whatman No. 2), and the filtrate was reduced in pressure by a rotary vacuum evaporator Concentrate in a concentrator (EYELH Co., Tokyo, Japan), freeze-dry at -70 ℃ in a freeze dryer, and store frozen.

The extract which simultaneously imparts the whitening activity and the wrinkle-reducing effect is composed of 0.1 to 50 wt% of the root root extract, 0.1 to 50 wt% of the caspase extract, 0.1 to 50 wt% of the goblet extract, and 0.1 to 50 wt% of the crude extract.

The extract that simultaneously imparts the whitening activity and the wrinkle-reducing effect is prepared at an optimal blending ratio of the components for enhancing the whitening effect and the wrinkle-reducing effect, and it is preferable that the components are blended according to the blending ratio given above.

Hereinafter, the technical composition of the cosmetic composition according to the present invention and the cosmetic composition containing the cosmetic composition will be described in detail with reference to examples.

Hereinafter, the technical composition of the cosmetic composition according to the present invention will be described with reference to Examples (1 to 3).

25 g of Seripolia ceraclarata extract, 25 g of Spirulina extract, and 50 g of Extract of Mushroom mushroom were mixed to prepare a cosmetic composition.

A cosmetic composition was prepared in the same composition as in Example 1,

5 g of the whitening active extract and 5 g of the wrinkle improving active extract.

That is, a cosmetic composition is prepared by mixing 25 g of Seripolya cerasata extract, 25 g of Spirulina extract, 40 g of Mushroom mushroom extract and 10 g of the above mixed extract.

The above whitening active extract is used in an amount corresponding to 50 wt% of 10 g composed of 3 g of an aqueous Sodium Hexafluoroarsenix extract, 3 g of a Kwangwoogwoo extract, 2 g of Saururus chinensis extract, and 2 g of a ginseng extract.

The wrinkle-improving active extract is used in an amount corresponding to 50wt% of 10g, which is a mixture of 3g of ginger berry extract, 3g of in-plant extract, 2g of blueberry extract, and 2g of motherwort extract.

A cosmetic composition was prepared in the same composition as in Example 1,

However, it further includes a mixed extract composed of 3 g of soft root extract, 3 g of caspase extract, 2 g of ganoderma extract, and 2 g of ganoderma extract.

That is, a cosmetic composition is prepared by mixing 25 g of Seripolya cerasata extract, 25 g of Spirulina extract, 40 g of Mushroom mushroom extract and 10 g of the above mixed extract.

Hereinafter, specific technical configurations of the cosmetic product according to the present invention will be described with reference to Examples (4 to 6).

Cosmetics manufactured using the cosmetic composition of Example 1

20 g of the cosmetic composition of Example 1, 2.5 g of lipophilic monostearic acid glycerin, 2.0 g of stearyl alcohol, 2.2 g of stearic acid, 1.0 g of wax, 1.5 g of polysorbate 60, 0.6 g of sorbitan stearate, 3.0 g of triethanolamine, 4.0 g of sodium hyaluronate, 0.01 g of preservative, 0.09 g of sodium chloride 0.01 g of pigment, 0.01 g of pigment, and 40.57 g of purified water.

Cosmetics manufactured using the cosmetic composition of Example 2

A nutritional cream is prepared in the same composition and the same mixing ratio as in Example 4, except that the cosmetic composition of Example 2 is used in selecting a cosmetic composition.

Cosmetics manufactured using the cosmetic composition of Example 3

A nutritional cream was prepared in the same composition and the same mixing ratio as in Example 4, except that the cosmetic composition of Example 3 was used in selecting a cosmetic composition.

[ Comparative Example 1 ]

Comparative Example 1 is a nutrition cream not containing the cosmetic composition according to the present invention,

3.5 g of lipophilic monostearic acid glycerin, 4.0 g of stearyl alcohol, 3.2 g of stearic acid, 2.0 g of wax, 2.5 g of polysorbate 60, 1.6 g of sorbitan stearate, 2.0 g of cured vegetable oil, 4.0 g of squalane, A mixture of 5.5 g of octanoin, 1.0 g of dimethicone, 1.1 g of sodium magnesium silicate, 6.5 g of glycerin, 4.0 g of betaine, 3.0 g of triethanolamine, 5.0 g of sodium hyrouronate, 0.01 g of preservative, 0.01 g of fragrance, 45.57 g of a nutrient cream is made by mixing.

[ Test Example 1 ]

The whitening effect of the cosmetics prepared in Examples 4 to 6 and Comparative Example 1

After diluting the cosmetic samples of Examples 1 to 6 and Comparative Example 1 in 50% dimethyl sulfoxide (DMSO), 50 μl each of the cosmetic samples was taken, and then 450 μl of 0.1 M phosphate buffer, tyrosinase (sigma ).

60 μl of 1.5 mM trypsin (Sigma) was added to the mixed solution, vortexed for 10 seconds, left at 40 ° C for 20 minutes, and absorbance was measured at 490 nm relative to the control (Arbutin).

The whitening activity at 40 캜 was measured to be 45.43% for Example 4, 48.2% for Example 5 and 45.3% for Example 6, and 13.15% for Comparative Example 1.

The wrinkle removing effect of the cosmetics manufactured in Examples 4 to 6 and Comparative Example 1

Fifty women in their 30s and 40s were enrolled. Fifty women before the experiment were asked to observe general characteristics through questionnaires, and skin tests were conducted to evaluate pigmentation, erythema, fine wrinkles, oil and water Self - skin care (cleansing, moisturizing, blocking) education program was conducted twice.

In the morning and evening, we applied cosmetics to the entire face for 6 weeks. After completing the experiment, we surveyed the satisfaction of the patients. As a result, we were asked about the improvement of skin condition. (48), 'Fine wrinkles were reduced (48)', 'Elasticity was increased (48)', 'Skin was smooth (48)' and 'Moistened (47)' There were many answers.

As a result, it was confirmed that the degree of satisfaction with the evaluation of the complexion clearness, the reduction of fine wrinkles, the increase of elasticity, and the increase of smoothness was high (47.3 ≒ 94.6%).

The composition of the present invention comprises the seripolylaceratta extract according to the present invention and the spirulina extract for improving skin whitening and wrinkles, and the extract of Zanthoxylum mushroom, and is highly industrially applicable since it has high skin whitening and wrinkle reducing effect.

Claims (5)

The mycelial culture broth was dried with a freeze drier, and the lyophilized seripolylacerase was pulverized using a crusher. The powder was dropped in an 80 to 120 mesh sieve at -15 ° C to -25 ° C, The cryoprecaryceratata powder was added to 80% methanol (MeOH) at a ratio of 1:20 to 30, and the mixture was extracted at room temperature for 3 to 5 days. The extracted extract was firstly filtered to obtain 3000 × gravity acceleration g) for 2 to 4 minutes, and a supernatant is obtained. In the cosmetic composition comprising the Seripolia cerasita extract,

Wherein said cosmetic composition comprises 0.01 to 70% by weight of the above-mentioned Seripolya cerasata extract;
Spirulina was mixed with distilled water having a weight of 30 to 50 times that of Spirulina and then shaken in an incubator at 20 to 27 ° C and 100 to 200 rpm for 65 to 80 hours and the shaken solution was stirred for 15 to 30 minutes using an ultrasonic disperser processor, and the dispersed solution is separated into supernatant and precipitate in a centrifuge at 6.000 to 9,000 rpm for 10 to 30 minutes, 0.01 to 70 wt% of spirulina extract obtained by taking the supernatant;
Dried pine mushroom was pulverized to 80 to 120 meshes using a pulverizer, and the granular mushroom powder and distilled water having a weight of 10 to 30 times the weight of the granular mushroom powder were mixed and then the pH was adjusted to 6.0 to 10.0 0.01 to 70% by weight of a mushroom extract extracted from 100 to 140 rpm for 5 to 20 hours at 90 to 100 캜;
The present invention provides a wrinkle-improving active extract which contains 0.1 to 99.9 wt% of a whitening active extract, which contains both red mudberry extract, Kwangwoowoo extract, Saururus chinensis extract and True Angelica keiskei extract, and Lycium chinense extract, 0.01 to 70 wt% of a 100 wt% mixed extract composed of 0.1 to 99.9 wt% of a mixture;
0.01 to 70 wt% of a 100 wt% mixed extract consisting of 0.1 to 50 wt% of a root extract, 0.1 to 50 wt% of a caspase extract, 0.1 to 50 wt% of a goat extract, and 0.1 to 50 wt% of a crude extract; ≪ / RTI >
The whitening active extract is 0.1 to 99.9 wt% of 100 wt% of a mixture of 0.1 to 50 wt% of an extract of Sasa extract, 0.1 to 50 wt% of a pine needle extract, 0.1 to 50 wt% of a Saururus chinensis extract, and 0.1 to 50 wt%
The wrinkle-improving active extract is 0.1 to 99.9 wt% of 100 wt% of a mixture of 0.1 to 50 wt% of an extract of cornflakes, 0.1 to 50 wt% of an extract of Inca extract, 0.1 to 50 wt% of a blueberry extract, and 0.1 to 50 wt% A cosmetic composition for skin whitening and wrinkle improvement.
delete delete delete A cosmetic composition for skin whitening and wrinkle reduction comprising the Seripolia ceraclara extract of claim 1 in an amount of 0.1 to 50 wt% based on the total weight of the cosmetic product. , Cosmetics for improving wrinkles.