KR102041419B1 - Cosmetics composition for improving and preventing skin damages and cosmetics including the same - Google Patents

Abstract

The present invention relates to a cosmetic composition capable of reducing scars, comprising natural extracts, and to a cosmetic product comprising the same. In particular, the cosmetic composition is effective in alleviating skin damage due to a first composition (A), and also effective in moisturization, antioxidant activities, skin protection, skin wrinkle reduction, whitening, and anti-aging functions due to a second composition (B). More specifically, the cosmetic composition consists of a mixture of the first composition (A) and the second composition (B). The first composition (A) comprises: an extract of green tea; an extract of bamboo leaves; an extract of sesame leaves; an extract of Lonicerae flos; an extract of laver; and an extract of wild parsley. The second composition (B) comprises: a humectant composition obtained by combining an extract of sea cucumber and an extract of Lonicerae flos in equal weight amounts; an antioxidant composition obtained by combining an extract of flaxseed and an extract of moringa leaves in equal weight amounts; a composition for skin protection and wrinkle reduction, obtained by combining an extract of black garlic and an extract of motherwort in equal weight amounts; a whitening composition obtained by combining an extract of persimmon tree leaves and an extract of bugbane flowers in equal weight amounts; and an anti-aging composition obtained by combining an extract of chestnut tree leaves and an extract of Rhododendron in equal weight amounts.

Description

Cosmetic composition having a function of improving skin wounds and cosmetics comprising the same {COSMETICS COMPOSITION FOR IMPROVING AND PREVENTING SKIN DAMAGES AND COSMETICS INCLUDING THE SAME}

The present invention green tea extract; Bamboo leaf extract; Sesame leaf extract; Gold coin extract; Seaweed extract; A first composition (A) having a skin wound improvement function is composed of a mixture of stone parsley extract;

A first mixed composition having a moisturizing function by combining sea cucumber extract and golden extract in the same weight ratio; A second mixture composition having an antioxidant function by combining flaxseed extract and vitamin tree leaf extract in the same weight ratio; A third mixed composition having a combination of black garlic extract and motherwort extract in the same weight ratio and having skin protection and anti-wrinkle function; A fourth mixed composition having a whitening function by combining persimmon leaf extract and horse riding flower extract in the same weight ratio; The composition of the second composition (B) comprising a chestnut leaf extract and the extract of the panacea extract in the same weight ratio and comprising a fifth mixture composition having an anti-aging function, thereby improving skin damage and moisturizing, antioxidant, skin protection And it relates to a cosmetic composition and a cosmetic comprising the same, which is expressed in combination with the wrinkle improvement, whitening, anti-aging effect.

Increasing the production of ROS in the cell by various factors such as fine dust in the air, heavy metals, ultraviolet rays, exhaust gases, electromagnetic waves, chemical oxidants and microorganisms, and inflammatory reactions may cause the antioxidant system to lose its balance and cause oxidative stress.

Oxidative stress causes oxidative damage at the cellular level, leading to various diseases as well as skin diseases.The result of oxidative damage due to ROS overproduction in the skin is melanin production, wrinkle formation, chronic and exacerbation of inflammation, wound healing. It appears as a problem such as delay.

In particular, the skin is a body that functions as a shield to protect the human body by being directly exposed to chemical, mechanical, and biological stimuli, and thus an inflammatory reaction associated with an immune response occurs frequently. In general, bioactive damage to the skin is usually wound on the epidermis or the upper dermis, and it naturally heals. However, when the physiological function of cells involved in the wound healing process of hemostasis, inflammation, and regeneration is not smooth, not only worsening of skin diseases but also aging promotes cosmetic and pathological problems in the skin.

The present invention relates to a cosmetic composition having a function of improving, moisturizing, antioxidant, skin protection and wrinkle improvement, whitening, and anti-aging of skin damaged by external environmental stress factors, and cosmetics using the same,

In this regard, Republic of Korea Patent Registration 10-0639408 (Date 2006.10.20) 'Cosmetic composition containing bamboo leaf extract having the skin damage inhibition and whitening effect by ultraviolet rays'; Republic of Korea Patent Application Publication No. 10-2017-0012989 (Published Date 2017.02.06) 'Cosmetic composition for improving skin damage resulting from fine dust or yellow dust containing peanut sprout extract'; Republic of Korea Patent Registration 10-1856480 (Registration date 2018.05.03) 'Cosmetic composition for preventing skin damage and relieving scalp irritation due to ultraviolet rays containing microalgae extract'; Republic of Korea Patent Registration 10-1828056 (Registration date 2018.02.05) 'Cosmetic composition for recovering skin damage due to UVB containing a natural extract as an active ingredient'; Republic of Korea Patent Application Publication No. 10-2016-0056744 (Published Date 2016.05.20) 'Fermentation cosmetic composition for promoting cell proliferation or damaged skin recovery comprising the mistletoe fermentation extract, hair root fermentation extract and soybean fermentation extract as an active ingredient'; This has been disclosed.

Conventional cosmetic compositions and cosmetics disclosed as described above has the disadvantage that it is unsuitable for the improvement of the damaged skin, moisturizing, antioxidant, skin protection and wrinkle improvement, whitening, anti-aging combined function.

Republic of Korea Patent Registration 10-0639408 (Registration Date 2006.10.20) Republic of Korea Patent Application Publication No. 10-2017-0012989 (Published date 2017.02.06) Republic of Korea Patent Registration 10-1856480 (Registration Date 2018.05.03) Republic of Korea Patent Registration 10-1828056 (Registration date 2018.02.05) Republic of Korea Patent Publication No. 10-2016-0056744 (Published Date 2016.05.20)

The present invention green tea extract; Bamboo leaf extract; Sesame leaf extract; Gold coin extract; Seaweed extract; A first composition (A) having a skin wound improvement function is composed of a mixture of stone parsley extract;

A first mixed composition having a moisturizing function by combining sea cucumber extract and golden extract in the same weight ratio; A second mixture composition having an antioxidant function by combining flaxseed extract and vitamin tree leaf extract in the same weight ratio; A third mixed composition having a combination of black garlic extract and motherwort extract in the same weight ratio and having skin protection and anti-wrinkle function; A fourth mixed composition having a whitening function by combining persimmon leaf extract and horse riding flower extract in the same weight ratio; The composition of the second composition (B) comprising a chestnut leaf extract and the extract of the panacea extract in the same weight ratio and comprising a fifth mixture composition having an anti-aging function, thereby improving skin damage and moisturizing, antioxidant, skin protection And it is an object of the present invention to provide a cosmetic composition and a cosmetic composition comprising the same, the wrinkles improvement, whitening, anti-aging effect is expressed in combination.

To achieve the above object,

In the present invention, the green tea leaves are chopped and dried in a dry oven at 55 to 65 ° C. for 2 to 4 days, and the dried green tea leaves and 70% ethanol are mixed at a weight ratio of 1:10 to 20, and then 58 to The green tea extract obtained by filtering the extract extracted at 62 ° C. for 3 to 5 hours with filter paper, and concentrating the filtrate under reduced pressure with a vacuum rotation evaporator for 5 to 7 hours under the conditions of 0.1 to 0.3 mPA. (a1) 5.0-40.0 wt%;

Bamboo leaf and water were mixed at a weight ratio of 1: 15 to 30, then extracted at 100 ° C. for 15 to 30 hours, and the filtrate was filtered with filter paper for 5 to 7 hours under conditions of 0.1 to 0.3 mPA. Bamboo leaf extract (a2) obtained by decompression concentration with a vacuum rotation evaporator for 5.0 to 40.0 wt%;

After cutting the sesame leaves, the sesame leaves and 80% methanol is mixed at a weight ratio of 1: 10 to 15, and then extracted by standing for 20 to 30 hours, and the filtrate obtained by filtering the extract with a filter paper is 0.1 to 0.3 mPA. Perilla leaf extract (a3) obtained by decompression concentration with a vacuum rotation evaporator for 5 to 7 hours under the condition of 5.0 to 40.0 wt%;

Gold and silver and purified water were mixed at a weight ratio of 1: 15 to 30, and then extracted by heating at 75 to 85 ° C. for 2 to 4 hours, and the filtrate was filtered with filter paper. 5.0 to 40.0 wt% of the sterling silver extract (a4) obtained by concentrating under reduced pressure with a vacuum rotation evaporator for ˜7 hours;

After mixing steam and 80% methanol in a 1: 5 to 10 weight ratio, immersion extraction for 20 to 25 hours at 40 ~ 50 ℃, the extract was centrifuged at 11,000 ~ 13,000 rpm for 20 to 40 minutes in a centrifuge The extract was taken, and the supernatant was filtered with filter paper, and the filtrate was concentrated under reduced pressure with a vacuum rotation evaporator for 5 to 7 hours under the conditions of 0.1 to 0.3 mPA (a5) 5.0. ˜40.0 wt%;

After mixing buttercups and 80% methanol in a weight ratio of 1: 10 to 20, extracting at 70 to 80 ° C. for 2 to 4 hours, and extracting the filtrate under reduced pressure for 5 to 7 hours under conditions of 0.1 to 0.3 mPA. First composition (A) 10.0 to 50.0 wt consisting of a mixture (a1 + a2 + a3 + a4 + a5 + a6) of stone parsley extract (a6) obtained by decompression concentration with a vacuum rotation evaporator (a6) 5.0-40.0 wt%; %Wow,

Sea cucumber and ethanol are mixed at a weight ratio of 1:10 to 12, and then extracted at a shaker incubator at 120 to 160 rpm at 65 to 75 hours at 24 to 26 ° C, and the extracted extract is 15 to 25 at 7,500 to 8,500 rpm in a centrifuge. The supernatant obtained by centrifugation for minutes was filtered through filter paper, and the filtrate was concentrated under reduced pressure for 5 to 7 hours under a vacuum rotation evaporator under a condition of 0.1 to 0.3 mPA, and

After mixing gold and methanol in a 1: 4 to 6 weight ratio, the mixture was refluxed at 65 to 75 ° C for 20 to 30 hours, and the supernatant obtained by centrifuging the extract at 3,500 to 4,500 rpm for 4 to 6 minutes was filtered. Filtration with a paper (filter paper), the first mixture of the golden extract obtained by concentrating the filtered extract under reduced pressure for 5 to 7 hours under the conditions of 0.1 ~ 0.3 mPA in a vacuum rotation evaporator in the same weight ratio (b1) 0.5-60.0 wt%;

Flaxseed powder and ethanol were mixed at a weight ratio of 1: 10 to 15, then immersed at 50 to 80 ° C. for 2 to 10 hours, and the extracted extract was filtered through filter paper and the filtrate was 58 to 63 ° C. Flaxseed extract obtained by decompression concentration with a vacuum rotation evaporator for 5 to 7 hours at

After mixing the vitamin tree leaf and ethanol in a weight ratio of 1:15 to 25, and then immersing at room temperature for 22 to 26 hours to separate the supernatant and the precipitate, and repeating the same process three times the filter paper (filter paper) The second mixed composition (b2) obtained by filtration and concentrating the filtrate under reduced pressure with a vacuum rotation evaporator at 58 to 63 ° C. for 5 to 7 hours at a weight ratio of 0.5 to 60.0 wt% ;

After mixing black garlic and distilled water at a weight ratio of 1: 5 to 30, shaking extraction for 1 to 5 hours at 100 ° C. and 100 to 140 rpm, and extracting the filtered extract with a filter paper and filtering the filtrate to 0.1 Black garlic extract obtained by decompression concentration with a vacuum rotation evaporator for 5 to 7 hours under the condition of ˜0.3 mPA;

After mixing motherwort and distilled water at a weight ratio of 1: 5 to 30, shaking extraction for 1 to 5 hours at 100 ° C. and 100 to 140 rpm, and extracting the obtained extract at 2,000 to 4,000 rpm for 10 to 30 minutes in a centrifuge. After centrifugation, the mother liquor extract obtained by depressurizing the supernatant with a filter paper and depressurizing the filtrate with a vacuum rotation evaporator for 5 to 7 hours under the conditions of 0.1 to 0.3 mPA in the same weight ratio 0.5 to 60.0 wt% of the third mixed composition (b3);

Persimmon leaf and ethanol were mixed at a weight ratio of 1: 5 to 30, and then reflux-cooled at 42 to 48 ° C. for 2 to 6 hours, and the extracted extract was centrifuged at 11,000 to 13,000 rpm for 20 to 40 minutes in an upper layer. The persimmon leaf extract obtained by extracting the filtrate and concentrating the filtrate with a filter paper and depressurizing the filtrate with a vacuum rotation evaporator for 5 to 7 hours under conditions of 0.1 to 0.3 mPA;

The riding flower and 70% ethanol were mixed at a weight ratio of 1: 5 to 30, shaken at 23 to 28 ° C. and 80 to 150 rpm for 40 to 55 hours, and the obtained extract was filtered with a filter paper. 0.5 to 60.0 wt% of the fourth mixed composition (b4) in which the filtrate was concentrated under reduced pressure with a vacuum rotation evaporator for 5 to 7 hours under a condition of 0.1 to 0.3 mPA, at a weight ratio;

After mixing chestnut leaves and ethanol at a weight ratio of 1: 10 to 15, and then immersing at room temperature for 22 to 26 hours to separate supernatant and precipitate, and repeating the same process three times and filtering the extracted extract with filter paper. Chestnut leaf extract obtained by concentrating the filtrate under reduced pressure with a vacuum rotation evaporator at 58-63 ° C. for 5-7 hours.

After mixing 1 to 20-22 weight ratio of panacea and ethanol, and immersed for 7 to 8 days at room temperature, the supernatant is separated and filtered three times, and 5 at the conditions of 0.1 ~ 0.3 mPA 0.5 to 60.0 wt% of the fifth mixed composition (b5) obtained by blending the extract of Manchurian herb obtained by decompression concentration with a vacuum rotation evaporator for ˜7 hours (b1 + b2 + b3 + b4 + b5); It provides a cosmetic composition having a skin wound improvement function is composed of a mixture (A + B) of 50.0 ~ 90.0 wt% of the second composition (B) of 100.0 wt% composed of).

In addition, the cosmetic composition provides a functional cosmetic prepared by adding 0.1 to 50.0 wt% based on the total weight of the cosmetic.

The cosmetic composition having a skin wound improvement function and a cosmetic comprising the same according to the present invention have the following effects.

first. Green tea extract; Bamboo leaf extract; Sesame leaf extract; Gold coin extract; Seaweed extract; A first composition (A) having a skin wound improvement function is composed of a mixture of stone parsley extract;

A first mixed composition having a moisturizing function by combining sea cucumber extract and golden extract in the same weight ratio; A second mixture composition having an antioxidant function by combining flaxseed extract and vitamin tree leaf extract in the same weight ratio; A third mixed composition having a combination of black garlic extract and motherwort extract in the same weight ratio and having skin protection and anti-wrinkle function; A fourth mixed composition having a whitening function by combining persimmon leaf extract and horse riding flower extract in the same weight ratio; The composition of the second composition (B) comprising a chestnut leaf extract and the extract of the panacea extract in the same weight ratio and comprising a fifth mixture composition having an anti-aging function, thereby improving skin damage and moisturizing, antioxidant, skin protection And it can provide a combination of wrinkle improvement, whitening, anti-aging effect.

second. Using natural extract, no skin irritation and stable on skin.

third. Herbal extracts; Moss extracts; Baekduong extract; By additionally adding the preservative functional composition, which is composed of a mixture of grapefruit seed extract, to the cosmetic composition, it is possible to use it more cleanly and safely due to the blocking of bacterial infection by the preservative functional composition having excellent antibacterial and antioxidant functions. It has the advantage of being able to extend.

Hereinafter, a detailed description of the above technical configuration will be described.

As mentioned above

The natural extract-containing cosmetic composition having a skin wound improvement function according to the present invention is chopped green tea leaves and dried for 2 to 4 days in a dry oven at 55 ~ 65 ℃, dried tea leaves and 70% ethanol 1: 10 to 20 by weight ratio, the extract extracted for 3 to 5 hours at 58 ~ 62 ℃ filtered with filter paper (filter paper), the filtrate is reduced pressure concentrator for 5 to 7 hours under the conditions of 0.1 ~ 0.3 mPA Green tea extract (a1) obtained by decompression concentration with a vacuum rotation evaporator (a1) 5.0 to 40.0 wt%;

Bamboo leaf and water were mixed at a weight ratio of 1: 15 to 30, then extracted at 100 ° C. for 15 to 30 hours, and the filtrate was filtered with filter paper for 5 to 7 hours under conditions of 0.1 to 0.3 mPA. Bamboo leaf extract (a2) obtained by decompression concentration with a vacuum rotation evaporator for 5.0 to 40.0 wt%;

After cutting the sesame leaves, the sesame leaves and 80% methanol is mixed at a weight ratio of 1: 10 to 15, and then extracted by standing for 20 to 30 hours, and the filtrate obtained by filtering the extract with a filter paper is 0.1 to 0.3 mPA. Perilla leaf extract (a3) obtained by decompression concentration with a vacuum rotation evaporator for 5 to 7 hours under the condition of 5.0 to 40.0 wt%;

Gold and silver and purified water were mixed at a weight ratio of 1: 15 to 30, and then extracted by heating at 75 to 85 ° C. for 2 to 4 hours, and the filtrate was filtered with filter paper. 5.0 to 40.0 wt% of the sterling silver extract (a4) obtained by concentrating under reduced pressure with a vacuum rotation evaporator for ˜7 hours;

After mixing steam and 80% methanol in a 1: 5 to 10 weight ratio, immersion extraction for 20 to 25 hours at 40 ~ 50 ℃, the extract was centrifuged at 11,000 ~ 13,000 rpm for 20 to 40 minutes in a centrifuge The extract was taken, and the supernatant was filtered with filter paper, and the filtrate was concentrated under reduced pressure with a vacuum rotation evaporator for 5 to 7 hours under the conditions of 0.1 to 0.3 mPA (a5) 5.0. ˜40.0 wt%;

After mixing buttercups and 80% methanol in a weight ratio of 1: 10 to 20, extracting at 70 to 80 ° C. for 2 to 4 hours, and extracting the filtrate under reduced pressure for 5 to 7 hours under conditions of 0.1 to 0.3 mPA. First composition (A) 10.0 to 50.0 wt consisting of a mixture (a1 + a2 + a3 + a4 + a5 + a6) of stone parsley extract (a6) obtained by decompression concentration with a vacuum rotation evaporator (a6) 5.0-40.0 wt%; %Wow,

Sea cucumber and ethanol are mixed at a weight ratio of 1:10 to 12, and then extracted at a shaker incubator at 120 to 160 rpm at 65 to 75 hours at 24 to 26 ° C, and the extracted extract is 15 to 25 at 7,500 to 8,500 rpm in a centrifuge. The supernatant obtained by centrifugation for minutes was filtered through filter paper, and the filtrate was concentrated under reduced pressure for 5 to 7 hours under a vacuum rotation evaporator under a condition of 0.1 to 0.3 mPA, and

After mixing gold and methanol in a 1: 4 to 6 weight ratio, the mixture was refluxed at 65 to 75 ° C for 20 to 30 hours, and the supernatant obtained by centrifuging the extract at 3,500 to 4,500 rpm for 4 to 6 minutes was filtered. Filtration with a paper (filter paper), the first mixture of the golden extract obtained by concentrating the filtered extract under reduced pressure for 5 to 7 hours under the conditions of 0.1 ~ 0.3 mPA in a vacuum rotation evaporator in the same weight ratio (b1) 0.5-60.0 wt%;

Flaxseed powder and ethanol were mixed at a weight ratio of 1: 10 to 15, then immersed at 50 to 80 ° C. for 2 to 10 hours, and the extracted extract was filtered through filter paper and the filtrate was 58 to 63 ° C. Flaxseed extract obtained by decompression concentration with a vacuum rotation evaporator for 5 to 7 hours at

After mixing the vitamin tree leaf and ethanol in a weight ratio of 1:15 to 25, and then immersing at room temperature for 22 to 26 hours to separate the supernatant and the precipitate, and repeating the same process three times the filter paper (filter paper) The second mixed composition (b2) obtained by filtration and concentrating the filtrate under reduced pressure with a vacuum rotation evaporator at 58 to 63 ° C. for 5 to 7 hours at a weight ratio of 0.5 to 60.0 wt% ;

After mixing black garlic and distilled water at a weight ratio of 1: 5 to 30, shaking extraction for 1 to 5 hours at 100 ° C. and 100 to 140 rpm, and extracting the filtered extract with a filter paper and filtering the filtrate to 0.1 Black garlic extract obtained by decompression concentration with a vacuum rotation evaporator for 5 to 7 hours under the condition of ˜0.3 mPA;

After mixing motherwort and distilled water at a weight ratio of 1: 5 to 30, shaking extraction for 1 to 5 hours at 100 ° C. and 100 to 140 rpm, and extracting the obtained extract at 2,000 to 4,000 rpm for 10 to 30 minutes in a centrifuge. After centrifugation, the mother liquor extract obtained by depressurizing the supernatant with a filter paper and depressurizing the filtrate with a vacuum rotation evaporator for 5 to 7 hours under the conditions of 0.1 to 0.3 mPA in the same weight ratio 0.5 to 60.0 wt% of the third mixed composition (b3);

Persimmon leaf and ethanol were mixed at a weight ratio of 1: 5 to 30, and then reflux-cooled at 42 to 48 ° C. for 2 to 6 hours, and the extracted extract was centrifuged at 11,000 to 13,000 rpm for 20 to 40 minutes in an upper layer. The persimmon leaf extract obtained by extracting the filtrate and concentrating the filtrate with a filter paper and depressurizing the filtrate with a vacuum rotation evaporator for 5 to 7 hours under conditions of 0.1 to 0.3 mPA;

The riding flower and 70% ethanol were mixed at a weight ratio of 1: 5 to 30, shaken at 23 to 28 ° C. and 80 to 150 rpm for 40 to 55 hours, and the obtained extract was filtered with a filter paper. 0.5 to 60.0 wt% of the fourth mixed composition (b4) in which the filtrate was concentrated under reduced pressure with a vacuum rotation evaporator for 5 to 7 hours under a condition of 0.1 to 0.3 mPA, at a weight ratio;

After mixing chestnut leaves and ethanol at a weight ratio of 1: 10 to 15, and then immersing at room temperature for 22 to 26 hours to separate supernatant and precipitate, and repeating the same process three times and filtering the extracted extract with filter paper. Chestnut leaf extract obtained by concentrating the filtrate under reduced pressure with a vacuum rotation evaporator at 58-63 ° C. for 5-7 hours.

After mixing 1 to 20-22 weight ratio of panacea and ethanol, and immersed for 7 to 8 days at room temperature, the supernatant is separated and filtered three times, and 5 at the conditions of 0.1 ~ 0.3 mPA 0.5 to 60.0 wt% of the fifth mixed composition (b5) obtained by blending the extract of Manchurian herb obtained by decompression concentration with a vacuum rotation evaporator for ˜7 hours (b1 + b2 + b3 + b4 + b5); 100.0 wt% of the second composition (B) of 50.0 to 90.0 wt% of the mixture (A + B).

<Composition 1>

The first composition is a green tea extract 5.0 ~ 40.0 wt%; Bamboo leaf extract 5.0-40.0 wt%; Sesame leaf extract 5.0 ~ 40.0 wt%; Sterling silver extract 5.0-40.0 wt%; Seaweed extract 5.0-40.0 wt%; Stone parsley extract 5.0 ~ 40.0 wt%; as a composition of the mixture, specific examples are shown in Table 1 below.

Formulation Example 1 Formulation Example 2 Formulation Example 3 Formulation Example 4 Formulation Example 5 Green tea extract 10.0 wt% 10.0 wt% 20.0 wt% 25.0 wt% 20.0 wt% Bamboo Leaf Extract 15.0 wt% 10.0 wt% 10.0 wt% 20.0 wt% 20.0 wt% Sesame Leaf Extract 20.0 wt% 15.0 wt% 10.0 wt% 10.0 wt% 15.0 wt% Gold Coin Extract 25.0 wt% 20.0 wt% 15.0 wt% 10.0 wt% 15.0 wt% Seaweed extract 20.0 wt% 25.0 wt% 20.0 wt% 15.0 wt% 15.0 wt% Stone Parsley Extract 10.0 wt% 20.0 wt% 25.0 wt% 20.0 wt% 15.0 wt% Sum 100.0 wt% 100.0 wt% 100.0 wt% 100.0 wt% 100.0 wt%

1. Green Tea Extract

Green-tea is a perennial seed plant belonging to the tea tree family.

Green tea is known to have pharmacological properties in the human body such as antibacterial, antioxidant, anticancer, antiallergic, lowering blood pressure, inhibiting melanin production, inhibiting skin aging, antidiabetic, diuretic and detoxification. Green tea is composed of various useful ingredients, especially caffeine, tannin, chlorophyII, vitamins vitamin-A, B1, B2, C, E and organic components amino acids, proteins, fats, fiber and inorganic components calcium, magnesium, copper , Manganese and other ingredients are various.

Green tea catechin is a natural antioxidant that has been reported to have various physiological activities such as anti-inflammatory, anti-allergic, and cancer. Among them, EGCG is the best antioxidant component that makes up more than 40% of polyphenols. It is reported that EGCG has a great effect on preventing fatal disease in modern people such as coronary artery disease and cancer.

The green tea extract was chopped green tea leaves and dried for 2 to 4 days in a dry oven (55 ~ 65 ℃ dry oven), after mixing the dry green tea leaves and 70% ethanol in a weight ratio of 10 to 20, 58 The extract obtained at ~ 62 ℃ for 3 to 5 hours was filtered through a filter paper (filter paper) and the filtrate was obtained by decompression concentration (vacuum rotation evaporator) for 5 to 7 hours under the conditions of 0.1 ~ 0.3 mPA under reduced pressure use.

More specifically,

The green tea leaves are cut into 1 cm × 1 cm and dried in a dry oven at 60 ° C. for 3 days. The dried green tea leaves and 70% ethanol are mixed at a weight ratio of 1: 10 and then dried at 60 ° C. The extract extracted for 4 hours is filtered, and the filtrate is concentrated under reduced pressure with a vacuum rotation evaporator for 6 hours under a condition of 0.2 mPA.

2. Bamboo Leaf Extract

Bamboo is a large woody grass that is distributed in the tropics and subtropics and is used as a building material, handicrafts, food and traditional medicines.

The major components of bamboo leaf extract are flavone glycosides, phenolic acids, coumarin lactones, anthraquinones and amino acids [9,24,26]. Many papers have reported that flavonoids abundant in bamboo leaf extract have various biological effects such as anti-free radicals, antioxidants, anti-aging, anti-fatigue, anti-bacterial and anti-viral, and cardiovascular diseases.

The bamboo leaf extract is mixed with bamboo leaf and water in a weight ratio of 1: 15 to 30, and then extracted for 15 to 30 hours at 100 ℃, the filtrate filtered the extract with a filter paper (filter paper) under the conditions of 0.1 ~ 0.3 mPA Use the one obtained by decompression concentration with a vacuum rotation evaporator for 5 to 7 hours.

More specifically,

Bamboo leaf and water were mixed at a weight ratio of 1:20, extracted at 100 ° C. for 20 hours, and the filtrate was filtered and concentrated under reduced pressure with a vacuum rotation evaporator at 0.2 mPA for 6 hours. .

3. Sesame Leaf Extract

Perilla frutescens var. Japonica Hara is an annual herbaceous plant belonging to Labiatae, native to China and East Asia.

Sesame leaves contain a large amount of anthocyanin pigments and flavonoids such as beta carotene, caffeic acid, luteolin, quercetin, and rosmarinic acid. It is known to contain.

The leaves of sesame leaves contain minerals such as calcium, iron, magnesium and phosphorus, and vitamins A and C, as well as phytonutrients such as tyrosine, lysine and linolenic acid. Sesame leaves have various physiological activities such as anti-inflammatory, anti-allergic, antimutagenic and antioxidant activity.

The sesame leaf extract is chopped sesame leaf, mixed with sesame leaf and 80% methanol in a weight ratio of 1:10 to 15, then left to extract for 20 to 30 hours, the filtrate filtered the extract with a filter paper (filter paper) It is used by concentration under reduced pressure with a vacuum rotation evaporator for 5 to 7 hours under the conditions of 0.1 ~ 0.3 mPA.

More specifically,

After cutting sesame leaves into 1 cm × 1 cm size, the sesame leaves and 80% methanol were mixed at a 1: 10 weight ratio, and left standing for 25 hours for extraction. The filtered filtrate was filtered for 6 hours under the condition of 0.2 mPA. Concentrated under reduced pressure using a vacuum rotation evaporator is used.

4. Gold Coin Extract

The main active substances of gold and silver (Lonicerajaponica Flos, LJF) are phenolic compounds, iridoids and flavonoids with various functional groups. It has the weakness of eliminating the fever of the body and eliminating the action of toxic substances, and is widely used in boils, syphilis, gonorrhea, hemorrhoids, tonsillitis and vasculitis.

The sterling silver extract is mixed with sterling silver and purified water in a weight ratio of 1: 15 to 30, and then heated and extracted at 75 to 85 ° C. for 2 to 4 hours, and the filtrate obtained by filtering the extract with a filter paper is 0.1 to 0.3 mPA. Under the conditions of 5 to 7 hours using a vacuum concentrated evaporator (vacuum rotation evaporator) obtained by decompression concentration.

More specifically,

Gold and silver and purified water were mixed at a weight ratio of 1:20, and then extracted by heating at 80 ° C. for 3 hours, and the filtrate was filtered under reduced pressure with a vacuum rotation evaporator for 6 hours under a condition of 0.2 mPA. do.

5. Seaweed Extract

Seaweed contains many different bioactive components that protect cells. Among various seaweeds, seaweed (Porphyra yezoensis) is a red algae plant and is a general term for seaweeds belonging to the purple hairs and the hairs. Seaweed is rich in vitamins, which promote skin metabolism and prevent pigmentation. It also contains a lot of carotene, which can help prevent cancer and prevent aging. It contains high quality protein and is rich in amino acids such as glutamic acid and allicin, which activates cell function and promotes brain function.

It is known as a mineral-rich food, such as folate, the main component of thyroid hormone, iron with hematopoietic action, and potassium to lower blood pressure. Seaweed contains antioxidants such as mycosporin-like amino acid (MAA) and polyphenol. MAA absorbs light in the ultraviolet region, 310-360 nm, due to the combination of cyclohexenone or cyclohexenimine chromophores in amino acids or imino alcohols. Absorption coefficient at maximum absorption wavelength is usually 28,100 ~ 50,000 M ^-1 cm ^-1 , water soluble and colorless.

The laver extract is mixed with laver and 80% methanol in a 1: 5 to 10 weight ratio, immersion extraction for 20 to 25 hours at 40 ~ 50 ℃, the extract for 20 to 40 minutes at 11,000 ~ 13,000 rpm in a centrifuge The supernatant was collected by centrifugation, and the filtrate obtained by filtering the supernatant with a filter paper was concentrated under reduced pressure with a vacuum rotation evaporator under a condition of 0.1 to 0.3 mPA for 5 to 7 hours. do.

More specifically,

After mixing steam and 80% methanol in a 1: 7 weight ratio, immersion extraction at 45 ℃ for 23 hours, the extract was centrifuged for 30 minutes at 12,000 rpm in a centrifuge to take a supernatant, and the supernatant was filtered The filtrate was concentrated under reduced pressure with a vacuum rotation evaporator for 6 hours at 0.2 mPA.

6. Parsley Extract

Stone parsley is a perennial herb belonging to the Apiaceae. Stone parsley contains nutrients such as proteins, fats, sugars, fiber, vitamins A, B1, B2, C, calcium, iron, etc. In particular, protein accounts for 30-40% of the total dry matter. In addition, it is rich in amino acids such as alanine, arginine, aspartic acid, glutamic acid, and glycine, and in addition, it contains vitamins such as ascorbic acid and minerals such as calcium, phosphorus and potassium.

The stone parsley extract is a mixture of stone parsley and 80% methanol in a weight ratio of 1: 10 to 20, and then extracted at 70 to 80 ℃ for 2 to 4 hours, the filtrate filtered the extract 5 to 7 under the conditions of 0.1 ~ 0.3 mPA The obtained by decompression concentration with a vacuum rotation evaporator for a time is used.

More specifically,

After mixing stone parsley and 80% methanol in a 1: 15 weight ratio, the mixture was extracted at 75 ° C. for 3 hours, and the filtrate was filtered and concentrated under reduced pressure using a vacuum rotation evaporator for 6 hours under a condition of 0.2 mPA. use.

<Composition 2>

The second composition is 0.5 ~ 60.0 wt% of the first mixed composition having a moisturizing function by combining the sea cucumber extract and the golden extract in the same weight ratio; 0.5 ~ 60.0 wt% of the second blend composition having antioxidant function by combining flaxseed extract and vitamin tree leaf extract in the same weight ratio; 0.5 ~ 60.0 wt% of a third mixture of black garlic extract and motherwort extract in the same weight ratio and having skin protection and wrinkle improvement functions; 0.5 ~ 60.0 wt% of the fourth mixed composition having a whitening function by combining persimmon leaf extract and horse riding flower extract in the same weight ratio; The chestnut leaf extract and the extract of Panaxaceae were mixed in the same weight ratio to form a mixture of 0.5 to 60.0 wt% of the fifth mixed composition having an anti-aging function, specific examples of the formulation are shown in Table 2 below.

Formulation Example 1 Formulation Example 2 Formulation Example 3 Formulation Example 4 Formulation Example 5 First mixture composition 10.0 wt% 25.0 wt% 15.0 wt% 30.0 wt% 20.0 wt% Second mixture composition 20.0 wt% 10.0 wt% 25.0 wt% 15.0 wt% 20.0 wt% Third mixture composition 30.0 wt% 20.0 wt% 10.0 wt% 25.0 wt% 20.0 wt% 4th mixed composition 15.0 wt% 30.0 wt% 20.0 wt% 10.0 wt% 20.0 wt% Fifth Mixed Composition 25.0 wt% 15.0 wt% 30.0 wt% 20.0 wt% 20.0 wt% Sum 100.0 wt% 100.0 wt% 100.0 wt% 100.0 wt% 100.0 wt%

1. First mixture composition

Sea cucumber is a general term for sea cucumbers belonging to the equatorial animal sea cucumber river. There are three types of sea cucumbers in Korea: red ginseng, blue ginseng, and black ginseng. Sea cucumbers are active against amino acids, such as cysteine, histidine, and lysine, and iron (Fe), phosphorus (P), and bile anemia, and hepatic exercise. It contains taurine, which has been shown to enhance gallstone dissolution and hepatic detoxification, lower cholesterol, improve heart function, and restore vision.

Raw sea cucumber contains water, protein, sugar, ash, calcium, phosphorus, iron, lysine, vitamins 1, B2, essential amino acids, iolysine tethionine, glutamic acid, glycine, tryptophan and taurine. Sea cucumber extract obtained from sea cucumber contains chondroitin, a form of mucopolysaccharide. Chondroitin is responsible for the accumulation of moisture and nutrients in the skin. Chondroitin sulfate is a mucopolysaccharide found in cartilage tissues, neural tissues and intercellular tissues, such as animals, as a main component of viscous polysaccharides. Chondroitin sulfate is free and does not exist in vivo, but binds to proteins to form chondroitin sulfate protein complex.It is the basis for normal survival of somatic cells such as collagen, elastin, and hyaluronic acid. Give elasticity. Chondroitin sulfate containing sea cucumber extract moisturizes by storing moisture in the skin, and also contains and stores amino acids, proteins, and iodine in large amounts to relieve wrinkles, prevent aging, skin whitening, cleanse, and have excellent effects on the skin. To have

The sea cucumber extract is mixed with sea cucumber and ethanol at a weight ratio of 1:10 to 12, and then extracted at a shaker incubator at 120 to 160 rpm at 65 to 75 hours at 24 to 26 ° C, and the extracted extract at 7,500 to 8,500 rpm in a centrifuge. The supernatant obtained by centrifugation for 15 to 25 minutes was filtered through a filter paper, and the filtrate was concentrated under reduced pressure for 5 to 7 hours under a condition of 0.1 to 0.3 mPA with a vacuum rotation evaporator. Use it.

As a specific example of the sea cucumber extract, 1,000 g of sea cucumber is put in 10% of 50% ethanol, and extracted by shaking at 160 rpm for 26 hours at 70 ° C. in a shaking incubator. The extract is centrifuged for 30 minutes at 7,000 rpm in a centrifuge. The supernatant obtained by centrifugation is filtered with Whatman No 3. filterpaper. The filtered extract was concentrated under reduced pressure at 62 ° C. and 0.2 mPA for 5 hours using a vacuum rotation evaporator to obtain 1 L of extract.

Golden (Scutellaria baicalensis) is a perennial herb belonging to the genus Cultivated dicotyledonous nectar and the thimble. It is called as a fund, a little, a bias, and the taste is bitter and the nature is cold and non-toxic. Medicinal roots are mainly used. Gold is known to be a powerful pharmacological crop and has been used as an alternative medicine for anti-inflammatory, anticancer and sedative effects.

The golden extract is obtained by mixing gold and methanol in a 1: 4 to 6 weight ratio, refluxing at 65 to 75 ° C. for 20 to 30 hours, and centrifuging the extract at 3,500 to 4,500 rpm for 4 to 6 minutes. The liquid was filtered through a filter paper, and the filtered extract was concentrated under reduced pressure for 5 to 7 hours under a condition of 0.1 to 0.3 mPA using a vacuum rotation evaporator.

As a specific example of the golden extract, after pulverizing the dried gold, 1,000 g of golden fine powder and 5 L of 70% ethanol are mixed, and reflux extraction at 72 ℃ for 15 hours.

The extract was centrifuged at 3,500 rpm for 10 minutes, and then the supernatant was obtained and filtered through Whatman No 3. filterpaper. The filtered extract was concentrated under reduced pressure at 62 ° C. and 0.2 mPA for 5 hours using a vacuum rotation evaporator to obtain 1 L of extract.

2. Second mixture composition

The linseed scientific name is Linum usitatissimum, a seed of the Linaceae plant. Flaxseed contains high amounts of vitamins and essential amino acids. However, there are three major beneficial ingredients that are rich in omega-3 fatty acids, lignans and dietary fiber.

Lignan has antioxidative properties, which make it a very useful antioxidant bioactive agent in the body. It has excellent anti-cancer, anti-dividing and anti-toxic properties, and has a strong function of inhibiting tumors already formed.

The flaxseed extract is mixed with flaxseed powder and ethanol in a weight ratio of 1:10 to 15, then immersed for 2 to 10 hours at 50 ~ 80 ℃, the extracted extract is filtered with a filter paper (filter paper), the filtrate The obtained by decompression concentration with a vacuum rotation evaporator for 5 to 7 hours at 58 ~ 63 ℃ is used.

Specific examples of the flax seed extract,

After drying, 1,000 g of ground flaxseed powder was immersed in 10 L of 95% ethanol, and then heated to 62 ° C for 3 hours. After the extract was filtered with a filter paper, the filtered extract was concentrated under reduced pressure at 62 ° C. and 0.2 mPA for 5 hours using a vacuum rotation evaporator to obtain 1 L of extract.

Vitamin tree (Hippophae rhamnoides L.) is a deciduous shrub belonging to the family Elaeagnacease, which is native to China and Mongolia. The leaves and fruits of the vitamin tree contain vitamins and amino acids, and are reported to have effects such as immune strengthening and anti-inflammatory action. Fruits contain polyphenols, tocopherols, carotenoids and flavonoids, and leaves are known to contain bioactive substances such as quercetin, gallic acid and tannin.

The vitamin tree leaf extract is a mixture of vitamin tree leaves and ethanol in a weight ratio of 1: 15 to 25, then immersed for 22 to 26 hours at room temperature to separate the supernatant and sediment, filter the extract extracted by repeating the same process three times Filtration was carried out with a filter paper, and the filtrate was concentrated under reduced pressure with a vacuum rotation evaporator at 58-63 ° C. for 5-7 hours.

Specific examples of the vitamin tree leaf extract,

After adding 1,000 g of vitamin tree leaf to 10 L of 50% ethanol and soaking for 25 hours at room temperature, the supernatant and the precipitate are separated. The mixture was extracted three times by repeating the same process, and the filtrate was concentrated under reduced pressure at 62 ° C. and 0.2 mPA for 5 hours using a vacuum rotation evaporator to obtain 1 L of extract.

3. Third mixture composition

When garlic is aged for a certain period of time in a high temperature thermostat, the garlic is changed to black by the garlic's own ingredients and enzymes, which is called black garlic.

While raw garlic is converted to black garlic, organic sulfur compounds such as S-allylcysteine (SAC), S-allylmercaptocystein (SAMC), tetrahydro-β-carboline, and the unstable and odorous components of raw garlic are changed into more stable and odorless water-soluble substances, It will contain fat-soluble substances such as diallyl sulfide (DAS), triallyl sulfide, diallyl disulfide (DADS) and diallyl polysulfides. S-allylcystein is a representative antioxidant isolated from black garlic, and fructosyl-arginine is a browning compound, which inhibits the production of thiobarbituric acid reactive substances (TBARS) in the liver and prevents cell damage caused by oxidized LDL. It is reported to be suppressed.

The black garlic extract is mixed with black garlic and distilled water in a weight ratio of 1: 5 to 30, and then shaken and extracted for 1 to 5 hours under conditions of 100 ° C. and 100 to 140 rpm, and the extracted extract is filtered through a filter paper. , The filtrate was obtained by decompression concentration with a vacuum rotation evaporator for 5 to 7 hours under the conditions of 0.1 ~ 0.3 mPA.

More specifically, 1,000 g of black garlic is added to 10 L of distilled water, followed by shaking extraction at 100 ° C. and 120 rpm for 2 hours. The obtained extract was filtered with a filter paper (Whatman No. 2), and the filtrate was concentrated under reduced pressure for 5 hours at 0.2 mPA with a vacuum rotation evaporator to obtain 1 L of extract.

Motherwort (Leonurus japonicus H.) is a biennial herb belonging to Labiatae. Its main ingredients are alkaloids such as leonurine, leonuridine, stachynose, stachydrine, and flavonoids, rutin as well as protocatechol, benzoic acid, vitamin A and fatty oils.

Motherwort juice not only activates the blood, but also removes toxic blood from the body, and the jugular vein, ie, high blood pressure, regulates the nervous pulse and detoxifies the postpartum blood. It is said that it is used to treat bleeding of the uterus, hematuria, bloody stool, hemorrhoids, large and urinary incontinence, etc.

The motherwort extract is mixed with motherwort and distilled water in a weight ratio of 1: 5 to 30, and then shaken for 1 to 5 hours under conditions of 100 ° C. and 100 to 140 rpm, and the obtained extract is centrifuged at 2,000 to 4,000 rpm. After centrifugation for 10 to 30 minutes, the filtrate obtained by filtering the supernatant with filter paper was concentrated under reduced pressure with a vacuum rotation evaporator for 5 to 7 hours under the conditions of 0.1 to 0.3 mPA. use.

More specifically, 1,000 g of dried motherwort powder is added to 15 L of distilled water, followed by shaking at 100 ° C. for 2 hours to obtain a supernatant centrifuged at 3,000 rpm for 15 minutes. The supernatant was filtered with filter paper (Whatman No. 2), and the filtrate was concentrated under reduced pressure for 5 hours at 0.2 mPA using a vacuum rotation evaporator to obtain 1 L of extract.

4. Fourth mixed composition

Persimmon tree (DiospyroskakiThunb) is a fruit tree with a long history and is native to temperate Asia, Korea, China and Japan. Persimmon leaves contain tannins, polyphenols, resins, coumarin compounds, organic acids such as betulic acid, oleanolic acid and ursolic acid, and flavonoid glycosides such as chlorophyll, astragalin and myricitrin, and vitamins A and C. It is reported that content of pantothenic acid and folic acid is large.

The persimmon leaf extract is a mixture of persimmon leaves and ethanol 1: 5 ~ 30 weight ratio, reflux-cooled extraction for 2 to 6 hours at 42 ~ 48 ℃, the extracted extract for 20 to 40 minutes at 11,000 ~ 13,000 rpm in a centrifuge The supernatant was collected by centrifugation, and the filtrate obtained by filtering the supernatant with a filter paper was concentrated under reduced pressure with a vacuum rotation evaporator under a condition of 0.1 to 0.3 mPA for 5 to 7 hours. do.

More specifically, the dried persimmon leaf is pulverized and 1,000 g of powdered persimmon leaf powder is added to 10 L of 80% ethanol and reflux-cooled at 45 ° C. for 6 hours. The obtained extract is centrifuged at 11,000 rpm for 40 minutes using a centrifuge to take the supernatant. The supernatant was filtered with filter paper (Whatman No. 2), and the filtrate was concentrated under reduced pressure for 5 hours at 0.2 mPA using a vacuum rotation evaporator to obtain 1 L of extract.

Horseback riding (Cimicifugae Rhizoma) refers to the root stem of horseback riding (Cimicifuga heracleifolia Komarov) belonging to Ranunculuaceae, which is harvested in summer and autumn to remove beard roots and sun-dried, and grows in Korea. Various types of 9,19-cyclolanostane glycosides were found in horseback riding, and Acetylshengmanol xyloside and cimicifugoside H-1 were also found in horseback riding. In addition, cimicifugin, khellol, amminol, caffeic acid, ferulic acid and other compounds are known.

The horse riding extract is a mixture of horse riding flowers and 70% ethanol in a weight ratio of 1: 5 to 30, and then shaken at 23 to 28 ℃, 80 to 150 rpm for 40 to 55 hours, and the obtained extract is filtered out of filter paper The filtrate filtered with) is concentrated under reduced pressure with a vacuum rotation evaporator for 5 to 7 hours under the conditions of 0.1 to 0.3 mPA.

More specifically, 1,000 g of the pulverized horse riding powder is added to 20 L of 70% ethanol and shaken at 26 ° C. and 120 rpm for 50 hours. The obtained extract was filtered with a filter paper (Whatman No. 2), and the filtrate was concentrated under reduced pressure for 5 hours at 0.2 mPA with a vacuum rotation evaporator to obtain 1 L of extract.

5. Fifth Mixed Composition

Chestnut tree (Castanea crenata) is a deciduous broad-leaved tree belonging to the oak family.

Studies on chestnut (Castanea crenata) leaves are currently being conducted in various ways, but the scope is not very broad.

The chestnut leaf extract is a mixture of chestnut leaves and ethanol in a weight ratio of 1:10 to 15, then immersed for 22 to 26 hours at room temperature to separate the supernatant and sediment, and the same process repeated three times to extract the filter paper ( filter paper) The filtrate was obtained by concentrating the filtrate under reduced pressure with a vacuum rotation evaporator at 58-63 ° C. for 5-7 hours.

Specific examples of the chestnut leaf extract,

1,000 g of chestnut leaves are added to 10 L of 70% ethanol and then immersed at room temperature for 24 hours. The supernatant and the precipitate are separated to obtain a supernatant, which is repeated twice. All the supernatants are mixed and filtered with filter paper.

The filtered extract was concentrated under reduced pressure for 5 hours at 62 ° C. and 0.2 mPA using a vacuum rotation evaporator to obtain 1 L of extract.

Rhododendron brachycarpum D.Don is an Rhododendron and is known by various names such as Cheonsangcho (Tencheoncho), Blackberry, Wind Foliage, and Lime Leaf. Manbyeongcho is a treatment for urinary tract pain, pain, joint pain, kidney pain caused by weak stomach and intestinal striated muscle spasms, kidney pain, vulgaris, menstrual irregularities, infertility, neuralgia, and high blood pressure. It is known to be effective as a tonic, diuretic. In addition, isolation of quercetin, avicularin, quercitrin, and hyperin among flavonoids was reported as a component of the leaf of the genus Panax.

The panacea extract is mixed with 1 to 20 to 22 weight ratio ethanol, immersed for 7 to 8 days at room temperature, the supernatant is separated and filtered three times, 0.1 ~ 0.3 mPA Under the conditions of 5 to 7 hours using a vacuum concentrated evaporator (vacuum rotation evaporator) obtained by decompression concentration.

As a specific example of the extract of the panacea,

1,000 g of Rhododendron is mixed with 10 L of 90% ethanol, and then immersed at room temperature for 7 to 8 days, and the supernatant is separated and filtered for three times. The filtrate was concentrated under a reduced pressure (vacuum rotation evaporator) at 62 ℃, 0.2 mPA for 5 hours to obtain 1 L of extract.

Cosmetic composition having a skin wound improvement function according to the present invention is 20.0 wt% green tea extract; Bamboo leaf extract 20.0 wt%; Sesame leaf extract 15.0 wt%; Sterling silver extract 15.0 wt%; Seaweed extract 15.0 wt%; 20.0 wt% of a first composition composed of a mixture of 15.0 wt% of parsley extract,

20.0 wt% of a first mixed composition comprising sea cucumber extract and golden extract in the same weight ratio; 20.0 wt% of a second mixture composition comprising flaxseed extract and vitamin tree leaf extract in the same weight ratio; 20.0 wt% of a third mixed composition comprising black garlic extract and motherwort extract in the same weight ratio; 20.0 wt% of a fourth mixed composition containing persimmon leaf extract and horse riding flower extract in the same weight ratio; Chestnut leaf extract and Manchurian extract in the same weight ratio of 50.0 wt% of the fifth mixture composition; the composition of the second composition of 80.0 wt% by mixing.

The cosmetic composition according to Example 2 is formulated to further improve the skin wound improvement function by further adding 0.1-10.0 wt% of the essence extract to the total weight of the cosmetic composition of Example 1.

In other words, the cosmetic composition of Example 1 is composed of a mixture of 90.0 to 99.9 wt% and 0.1-10.0 wt% of the main extract. Specifically, it consists of a mixture of 95.0 wt% of the cosmetic composition of Example 1 and 5.0 wt% of the main extract.

The extract extract after mixing the dried extract and 70% ethanol in a weight ratio of 1: 10 to 20, shake extraction for 40 to 50 hours at a condition of 80 ~ 150 rpm, 23 ~ 28 ℃ in a shake extractor, extract the filter paper The filter paper was filtered and the filtrate was concentrated under reduced pressure with a vacuum rotation evaporator at 58 to 63 ° C. for 5 to 7 hours.

More specifically,

The dry solid and 70% ethanol were mixed at a weight ratio of 1:15, followed by shaking extraction in a shake extractor at 100 rpm and 25 ° C. for 45 hours. The extract was filtered with filter paper, and the filtrate was filtered at 60 ° C. The obtained by decompression concentration with a vacuum rotation evaporator for 6 hours is used.

The Angelica tenuissimae Radix is a perennial plant belonging to the Umbelliferae, which sprouts in mid-April and blooms in late August. The pharmacological effect of Gobon is an analgesic and analgesic drug that is effective for pain due to head pain, back pain, and gynecological diseases. Anti-cancer and mutagenesis inhibitory effects, cancer cell growth inhibition effect, cytotoxicity and hypoglycemic effect on normal cells, blood pressure control, thrombolytic activity in essential oils.

The cosmetic composition according to Example 3 is formulated to further improve the skin wound improvement function by further adding 0.1 to 10.0 wt% of the Gardenia extract to the total weight of the cosmetic composition of Example 1.

That is, it is composed of a mixture of 90.0 to 99.9 wt% of the cosmetic composition of Example 1 and 0.1 to 10.0 wt% of the gardenia extract. Specifically, it consists of a mixture of 95.0 wt% of the cosmetic composition of Example 1 and 5.0 wt% of the gardenia extract.

The gardenia extract is mixed with powdered gardenia fruit and distilled water in a weight ratio of 1: 20 to 40, and then refluxed at 100 ° C. for 20 to 40 minutes by a heating circulation extraction method, and extracts are extracted from filter paper. The filtrate was obtained by concentrating the filtrate under reduced pressure with a vacuum rotation evaporator at 58 to 63 ° C. for 5 to 7 hours.

More specifically,

Gardenia crushed powder and distilled water were mixed at a weight ratio of 1:25, and the mixture was extracted under reflux for 30 minutes at 100 ° C by a heating circulation extraction method, the extract was filtered with filter paper, and the filtrate was filtered at 60 ° C. The obtained by decompression concentration with a vacuum rotation evaporator for 5 hours is used.

Gardenia (Gardeniae fructus) is the fruit of Gardenia jasminoides Ellis, which belongs to the Rubiaceae. Gardenia has been used as a dye for food coloring since ancient times, but in clinical herbal medicine, it has been classified as a blue heat extinguishing agent and has shown an excellent effect on antipyretic action. In addition, it promotes bile secretion, treats fever of large intestine, and is used for improvement of symptoms such as nausea, fevers, anti-inflammatory, detoxification, hemostasis, soothing, pain relief, swelling, pain, and bleeding when there is sprain or damage to limb joint. come.

The main components of gardenia are crocin, Flavonoid-based gardenin, and iridoid glycoside, genipin, geniposide, and gardenoside.

The cosmetic composition according to Example 4 is further added to 0.1-10.0 wt% of Angelica extract based on the total weight of the cosmetic composition of Example 1 to further improve the skin wound improvement function.

That is, it is composed of a mixture of 90.0 to 99.9 wt% of the cosmetic composition of Example 1 and 0.1 to 10.0 wt% of the Angelica extract. Specifically, it consists of a mixture of 95.0 wt% of the cosmetic composition of Example 1 and 5.0 wt% of Angelica extract.

The Angelica gigantis Radix is a perennial genus belonging to the family Umbeliferae. Angelica gigas Nakai refers to the donkey that grows in Korea and is collected by drying roots before flowering.

The main pharmacological ingredient is decursin, a coumarin derivative, and decursinol, umbelliferon, and β-sitosterol. Main components include ligustilide, butylidenphthalide, etc. Peripheral vasodilation, anti-asthma, jingyeong function.

The cosmetic composition according to Example 5 is formulated to further improve the skin wound improvement function by further adding 0.1 ~ 10.0 wt% peppermint extract based on the total weight of the cosmetic composition of Example 1.

That is, it is composed of a mixture of 90.0 to 99.9 wt% of the cosmetic composition of Example 1 and 0.1 to 10.0 wt% of peppermint extract. Specifically, it is composed of a mixture of 95.0 wt% of the cosmetic composition of Example 1 and 5.0 wt% of the peppermint extract.

The peppermint extract is mixed with peppermint and 80% ethanol in a weight ratio of 1: 10 to 20, immersed and extracted for 20 to 30 hours at 55 to 65 ℃, the extract is filtered through a filter paper, and the filtrate is 58 to Use obtained by depressurizing concentration with a vacuum rotation evaporator for 5 to 7 hours at 63 ℃.

More specifically,

Peppermint and 80% ethanol were mixed at a weight ratio of 1: 15, then immersion extraction at 60 ° C. for 25 hours, the extract was filtered with filter paper, and the filtrate was dried at 60 ° C. for 5 hours under a vacuum rotation evaporator. ) Is obtained by concentration under reduced pressure.

The mint (Mentha arvensis) is a herbaceous plant, the essential oil extracted has been used for a long time in aromatherapy and has an excellent effect on fatigue recovery. In addition, it has been used as a medicine because it is effective in preventing skin diseases. Efficacy is used for fever, headache, sweating due to external cold, and it is effective for eye redness, sore throat, tonsillitis, skin itch, liver disease, abdominal pain, diarrhea and vomiting. Anti-inflammatory, healthy stomach, bile secretion and gastrointestinal smooth muscle suppression, increased mucus secretion of the respiratory tract, central nervous system excitement, uterine contraction, and the like has been described a variety of efficacy.

The cosmetic composition according to Example 6 is further added to 0.01 to 3.0 wt% of the storage functional composition based on the total weight of the cosmetic composition of Example 1, so as to have a shelf life extending effect.

The preservative functional composition is composed of a mixture of 0.01 ~ 50.0 wt% of the extract of the vinegar, 0.01 ~ 50.0 wt% of the moss extract, 0.01 ~ 50.0 wt% of Baekduong extract, 0.01 ~ 50.0 wt% of grapefruit seed extract, It is composed of a mixture of 25.0 wt% of the extract of the cabbage, 25.0 wt% of the moss extract, 25.0 wt% of the baekduong extract, 25.0 wt% of the grapefruit seed extract.

The specific blending ratio of the cosmetic composition according to Example 6 consists of a blending ratio of 98.0 wt% of the cosmetic composition of Example 1 and 2.0 wt% of the storage functional composition.

The preservative functional composition has a function of extending the shelf life of the cosmetic composition and cosmetics as a natural preservative composed of a mixture of the extract, moss extract, baekduong extract, grapefruit seed extract.

Japonica (Zanthoxylum schinfolium) is a species that grows wild in the field below 1,000m in Korea and its fruit is used as cooking oil resource and various seasonings, and seed oil is also used as lubricating oil of appliance such as water seed.

Sancho is the fruit of the Japanese vinegar, and the fruit peel contains 2 to 4% of essential oils. The main ingredients are dipentene (㎗-limonene), citronellal, ℓ-β-phellandrene, geraniol, citronellol, etc. Was done. The fruit contains xanthoxin, which is a sexually transmitted poison, xanthoxic acid, which is a paralytic component, sterol campesterol, stigmasterol, and β-sitosterol.

The extract was extracted with a mixture of methanol and methanol in a weight ratio of 1: 10 to 15, and then stirred at room temperature for 22 to 26 hours, the extracted extract was filtered with a filter paper, and the filtrate was 0.1 ~ 0.3 mPA Under the conditions of 5 to 7 hours using a vacuum concentrated evaporator (vacuum rotation evaporator) obtained by decompression concentration.

As a specific example of the extract, the extract was mixed with stirring 1000 g of acid and 10 L methanol (methanol) for 24 hours at room temperature. Extract was extracted using Filter paper No. Filtration at 2 and concentration under reduced pressure with a vacuum rotation evaporator at 62 ° C. and 0.2 mPA for 5 hours yielded 1 L of extract.

Moss has been reported to have excellent antibacterial and anti-oxidant effects, and when moss is put in water, the acidity of the surrounding water is increased by the body material of the moss, which reduces the activity of bacteria and prevents the deterioration of water.

There are lichens (moses, moss, Musci), umbrella lichens (tears, liverwort, Hepaticae), and horned lichens (horns, hornwort, Anthocerotae). These moss are highly useful as natural preservatives by suppressing microbial propagation and antioxidant activity.

The moss extract is crushed after hot air drying at 55 ~ 65 ℃ after washing the moss, and mixed with the crushed moss and 100% methanol in a weight ratio of 1: 10 ~ 15, 58 ~ in reflux cooling apparatus attached to the cooling tube. The mixture was cooled to reflux for 5 to 7 hours at 62 ° C., and the extracted extract was filtered through a filter paper, and the filtrate was concentrated under reduced pressure with a vacuum rotation evaporator for 5 to 7 hours at a condition of 0.1 to 0.3 mPA. Obtained by use of.

As a specific example of the moss extract, the moss mixture is mixed with brush moss, large feather moss, hair feather moss in the same weight ratio, washed with water and dehydrated and then pulverized by hot air drying at 62 ℃ for 6 hours. 1,000 g of the pulverized dry sample and 10 L of 100% methanol were mixed in a reflux condenser with a cooling tube, mixed, and reflux-cooled at 58 ° C. for 7 hours.

The extract was filtered through a filter paper having a pore size of 5 μm, and concentrated under reduced pressure with a vacuum rotation evaporator at 62 ° C. and 0.2 mPA for 5 hours to obtain 1 L of extract.

Baekduong (P ulsatilla k oreana Nakai et M ori) is a perennial plant belonging to the Ranunculaceae family and is wild throughout the country and distributed in northeast China. This herb has been commonly applied to toothache, antipyretic, and species, and has been used for anti-inflammatory, astringent, hemostatic, dysentery, clear detoxification, scrotum, antibacterial, soothing, and anticancer. Since ancient times, this material has been widely used in Korea for the purpose of anti-inflammatory, analgesic, antipyretic and detoxification and has been reported to have antibacterial effect.

The baekduong extract is washed with baekduong and dried in a lyophilizer, then processed into a powder form, and mixed with baekduong and powdered distilled water in a weight ratio of 1:10 to 12 times, then 2 to 4 at 75 to 85 ° C. Reflux extraction for a time, the extracted extract is filtered through a filter paper (filter paper), and the filtrate is obtained by decompression concentration (vacuum rotation evaporator) for 5 to 7 hours under the conditions of 0.1 ~ 0.3 mPA to use the obtained.

As a specific example of the baekduong extract, after mixing 1,000 g of baekduong in powder form with 10 L of distilled water to form a mixture, the mixture is refluxed at 80 ° C. for 3 hours. The extract is filtered with filter paper.

The filtered extract was concentrated under reduced pressure for 5 hours at 62 ° C. and 0.2 mPA using a vacuum rotation evaporator to obtain 1 L of extract.

Grapefruit is a deciduous tree with evergreen and evergreen arbor, which is succulent and slightly acidic. Grapefruit seed extract is a natural antibacterial substance, and its main ingredients include ascorbic acid, palmitic acid, glucose, and naringin ricotocopherol, which inhibit the growth of mycotoxin and mycotoxin biosynthesis.

The grapefruit seed extract is the mixture of grapefruit seed and ethanol 1: 5 to 10 by weight ratio, reflux cooling extraction for 2 to 3 hours at a temperature condition of 60 ~ 62 ℃, the extracted extract as a filter paper (filter paper) The filtrate was obtained by centrifugation of the filtrate at 10,000 × g for 20 to 40 minutes, and the obtained supernatant was concentrated under reduced pressure for 5 to 7 hours under a condition of 0.1 to 0.3 mPA using a vacuum rotation evaporator.

As a specific example, grapefruit seed and ethanol were mixed at a weight ratio of 1: 8, reflux cooled for 2 hours at a temperature of 60 ° C., the extracted extract was filtered with a filter paper, and the filtrate was 10,000 × g. The supernatant obtained by centrifugation for 30 minutes at was used by vacuum concentration evaporator (vacuum rotation evaporator) obtained under reduced pressure for 5 hours under the condition of 0.2 mPA.

DI water 703.15 g, Carbopol 2.0 g, Glycerine 50.0 g + Xanthan gum 0.05 g, Allantoin 2.0 g, Betaine 5.0 g , 1.0 g of arginine (L-Arginine), trehalose (Trehalose) 30.0 g, adenosine (Adenosine) 0.4 g is mixed to form an aqueous phase.

2. 5.0 g of Lipoid S75-3, 5.0 g of Glycerol Monostearate-105 (GMS 105), 5.0 g of Bees Wax, 10.0 g of Cetearyl Alcohol, 10.0 g of Shea Butter g, Phenoxyethanol 10.0 g, Caprylic / Capric triglyceride (MCT) 50.0 g, Tween # 60 15.0 g, Grape Seed Oil 15.0 g, Sweet Almond 25.0 g of oil and 1.0 g of methyl parahydroxy benzoate are dissolved by heating to form an oil phase.

3. The oil phase is mixed with the water phase to form a mixture.

4. When the temperature of the mixture falls below 38 ° C., 50 g of the cosmetic composition of Example 1, 5.0 g of cyclomethicone (DC345), and 0.4 g of fragrance are added to the mixture, followed by stirring to complete the lotion.

In Comparative Example 1, the amount of purified water (D.I Water) was 753.15 g, and the lotion was completed without using the cosmetic composition according to the present invention.

This embodiment is to prepare a lotion through the same process as in Example 7, but in using a cosmetic composition, the lotion is prepared using 50.0 g of the cosmetic composition of Example 2.

This embodiment is to prepare a lotion through the same process as in Example 7, but in using a cosmetic composition, to prepare a lotion using 50.0 g of the cosmetic composition of Example 3.

This embodiment is to prepare a lotion through the same process as in Example 7, but in using a cosmetic composition, the lotion is prepared using 50.0 g of the cosmetic composition of Example 4.

This embodiment is to prepare a lotion through the same process as in Example 7, but in using a cosmetic composition, the lotion is prepared using 50.0 g of the cosmetic composition of Example 5.

This embodiment is to prepare a lotion through the same process as in Example 7, but in using a cosmetic composition, using a 50.0 g of the cosmetic composition of Example 6 to prepare a lotion.

[Test Example 1]

Antioxidant Measurement

To measure the antioxidant effect of the lotion according to Examples 7 to 12.

After diluting the lotion samples of Examples 7-12 and Comparative Example 1 in 50% dimethyl sulfoxide (DMSO), 80 μl of each lotion sample was taken, and then 10mM DPPH (1,1-diphenyl-2) was added to the cosmetic sample. After adding 280 μl of -picrylhydrazyl) solution for 15 seconds and vortexing for 15 seconds, 1 mL of distilled water was added thereto, and the absorbance was measured at 517 nm for 35 minutes.

As a result of measuring antioxidant activity at 40 degreeC,

In Example 7, 52.74%, Example 8 was 56.15%, Example 9 was 52.27%, Example 10 was 53.77%, Example 11 was 52.34%, and Example 12 was 53.20%. Comparative Example 1 was determined to be 11.29%.

The antioxidant effects of the cosmetics according to Examples 7 to 12 were confirmed in the order of Example 8> Example 10> Example 12> Example 7> Example 11> Example 9. Example 9 to Example 12 shows a slightly different antioxidant activity depending on the composition, but it can be confirmed that it has a higher antioxidant effect than Comparative Example 1.

[Test Example 2]

<Antibacterial measurement>

The antimicrobial effect of the lotions of Examples 7-12 is to be measured.

Antimicrobial tests were performed on the pathogenic strains Staphylococcus aureus KCCM 11335, Escherichia Coli, Pseudomonas aeruginosa KCCM 11802, Candida albicans KCCM 11282, and Aspagillusniger KCCM 60143.

Gram-positive bacteria Staphylococcus aureus (S. aureus, KCCM 11335), Gram-negative bacteria Pseudomonas aeroginosa (P. aeroginosa, KCCM 11802), Escherichia coli (E. coli, KCCM 40271), Yeast Candida albicans (C.albicans KCCM 11282), The bacteria Aspergillus niger (A. niger KCCM 60143) was incubated, but the bacteria were incubated in a shaking incubator (37 ℃, fungi 26 ° C, 250 rpm) controlled.

The viable bacterial strains cultured in this way were inoculated into the cosmetic products of Examples 7 to 12, and the number of viable cells was measured on 1, 3, and 7 days, respectively. It was not detected.

[Test Example 3]

<Wrinkle improvement effect measurement>

1. Measurement of Elastase Inhibition Rate

Activity was increased by reaction of N-succinyl- (Ala) 3-p-nitroanilide with elastase. The inhibition level was measured by IC50 (㎍ / ㎖) at a concentration of 50% decrease in activity.

To 200 μl of substrate 1.0 mM N-succinyl- (Ala) 3-p-nitroanilide, 20 μl of sample solution diluted with 500 μg / ml of each of the cosmetic compositions of Examples 1 to 6 and 10 μl of 2.5U Elastase were added. After reacting at 25 ° C. for 10 min, the absorbance was measured at 410 nm with an ELISA reader. Elastase inhibition rate is expressed as the absorbance reduction rate of the addition and no addition of the sample solution as follows.

% Inhibition = {1-(absorbance of sample addition / absorbance of no addition)} × 100

As a result, the inhibition of the control group (Ursolic acid used) was 40.25%, the inhibition rate of Example 1 was 51.07%, the inhibition rate of Example 2 was 55.55%, the inhibition rate of Example 3 was 51.31%, and the inhibition rate of Example 4 was The inhibition rate of 53.04%, Example 5 was 54.22%, and the inhibition rate of Example 6 was 54.34%.

2. Measurement of Collagenase Inhibition Rate

The degree of inhibition was measured by IC ₅₀ (µg / ml) at a concentration of 50% decrease in activity by evaluating the activity of Collagenase to degrade Collagen.

Collagen was dissolved in a buffer solution of 20 mM Tris-HCl (pH 7.5) and 5 mM CaCl 2 to make 3 μg / ml substrate solution. 200 μl of buffer, 20 μl of substrate solution, and 20 μl of sample solution diluted with 500 μg / ml of each of the antiwrinkle cosmetic compositions of Examples 1 to 5 were added thereto, followed by reacting at 37 ° C. for 1 hour by adding Collagenase. After 100 min of 2% ninhydrin reagent was added and reacted for 20 minutes, the absorbance was measured at 570 nm with an ELISA reader.

As a result, the inhibition rate of Example 1 was 72.84%, the inhibition rate of Example 2 was 74.42%, the inhibition rate of Example 3 was 80.20%, the inhibition rate of Example 4 was 78.34%, the inhibition rate of Example 5 was 79.38%, and Example 6 Inhibition rate of 79.57% was confirmed.

It was confirmed through the results of the Elastase inhibition rate and Collagenase inhibition rate of the cosmetic composition of Examples 1 to 6 has a wrinkle improvement effect, thereby predicting the wrinkle improvement effect of the cosmetic composition containing the cosmetic composition.

[Test Example 4]

<Measurement of whitening effect>

Melanocytes present in the basal layer of the skin protect the skin by synthesizing melanin from tyrosine, which is stimulated by ultraviolet light. However, excessive production of melanin can cause aesthetic and skin health problems, so it is necessary to protect the skin from ultraviolet rays or to inhibit melanin production.

In order to examine the whitening efficacy of cosmetics, 12 women aged 20 to 40 years without skin disease were selected as test subjects, and tyrosinase activity inhibition rate measurement and melanin production inhibition measurement were performed.

1. Determination of tyrosinase activity inhibition rate

Tyrosinase is the only enzyme involved in the biosynthesis of melanin, the skin's pigment. The formula for calculating the inhibition rate of tyrosinase activity is as follows.

% Inhibition of tyrosinase activity

= 100-[(b-b ') / (a-a')] × 100

a: absorbance after reaction of blank sample liquid

b: absorbance after reaction of the sample liquid

a ', b': absorbance measured by substituting buffer for tyrosinase

Mushroom tyrosinase was added to a mixed solution prepared by separately adding 0.5 ml of 0.02 M sodium phosphate buffer (pH 6.5), 0.2 ml of 10 mM L-DOPA substrate solution and 0.1 ml of the cosmetic composition selected in Examples 1 to 6 to a 96 well plate. 110 Unit / mL) 0.2 ml was added and reacted at 37 ° C. for 2 minutes, and the absorbance was measured at 475 nm.

Arbutin was used as a control as a comparative example.

In order to confirm the inhibitory effect of tyrosinase, instead of the cosmetic composition according to Examples 1 to 6, arbutin (comparative example) used in non-medicinal products and cosmetics as a whitening agent, and in Examples 1 to 6 The whitening activity of the cosmetic composition was compared.

The whitening activity of 0.1% albutin as a comparative example was measured to be 68.53%.

The whitening activity of Example 1 was 60.27%; The whitening activity of Example 2 was 62.33%; The whitening activity of Example 3 was 61.28%; The whitening activity of Example 4 was 61.81%; The whitening activity of Example 5 was 58.37%; The whitening activity of Example 6 was 60.85%; showing a high activity almost similar to the arbutin (albutin) used in quasi-drugs and cosmetics as a whitening agent.

Therefore, it can be seen that the whitening effect of the cosmetic composition according to Examples 1 to 6 according to the present invention is large.

2. Inhibiting Melanin Production

B16 melanoma cells were aliquoted into 3 ml into 6 well plates and incubated for 12 hours in phenol red-free DMEM solution containing 10% FBS.

The cosmetic compositions according to Examples 1 to 6 were each pretreated by incubating at 36 ° C. for 12 minutes, and then treated with 1 uM of MSH and incubated at 37 ° C. for 48 hours.

After incubation, add 100 uL of 10 mM sodium phoshate buffer (pH 6.8) containing 1% (w / v) Triton X-100, shake for 5 minutes, and transfer to an eppendorf tube.

100 uL of 1 N NaOH and 200 uL of distilled water were added to the cell pellet obtained by centrifugation at 3,000 rpm for 5 minutes, incubated at 60 ° C for 1 hour to completely dissolve melanin, and then transferred 200 uL to a 96 well plate and measured absorbance at 405 nm. It was.

Absorbance at 490 nm was measured and compared using a Microplate Reader (Infinite 200, Tecan Group Ltd., Switzerland).

Inhibition (%) = [1- (A1 / A0)] × 100

A0: Absorbance control of non-added furniture

A1: Absorbance of Sample Addition

As a result, the inhibitory activity of Example 1 was 58.57%; Inhibitory activity of Example 2 was 63.24%; Inhibitory activity of Example 3 was 63.33%; Inhibitory activity of Example 4 was 63.42%; Inhibitory activity of Example 5 was 63.57%; Inhibitory activity of Example 6 was found to be 63.37%; showed excellent inhibitory ability.

It can be expected that the whitening effect will be great when the cosmetic composition according to the present invention is applied to the tyrosinase activity (tyrosinase activity) inhibition rate measurement results and melanin (Melanin) production results.

[Test Example 5]

Anti Aging Measurement

To measure the anti-aging effect of the cosmetic composition according to Examples 1 to 6.

Skin aging due to photoaging during skin aging is caused by irregular pigmentation, skin sagging, vasodilation, decreased elasticity, blistering and deep and thick wrinkles caused by UV exposure, and the skin becomes very rough and dry. In particular, the amount of collagen in the skin is reduced and elastic fibers are denatured to cause wrinkles.

The action of ultraviolet light causes the skin to over-produce harmful reactive oxygen species (ROS), including superoxide anions, peroxides, singlet oxygen, and other antioxidants. Enzymes and low molecular weight antioxidants such as glutathione, vitamin E, vitamin C and ubiquinol are reduced. Oxidative stress causes lipid peroxidation, protein oxidation, activation of proteolytic enzymes that destroy epileptic components, breakdown of collagen and elastin, elastic fibers, and damage of cellular components, and promote photoaging.

The reactive oxygen species (ROS) refers to oxygen having free radicals as an intermediate product generated when oxygen molecules are incompletely reduced during cell respiration. ROS occurs naturally by the normal metabolism of oxygen and plays an important role in cell signaling and homeostasis. The concentration of ROS can increase dramatically due to environmental stresses such as exposure to ultraviolet light or high heat.

Increasing ROS can lead to oxidative stress, leading to diseases such as skin aging and pigmentation.

In order to determine the anti-aging effect of the cosmetic composition according to Examples 1 to 6, an experiment was performed using HaCaT cells (ATCC, Manassas, VA, USA), which are human keratinocytes.

Normal medium (Comparative Example 1) was placed in HaCaT cells, or cultured in a medium in which 100 μg / ml of the cosmetic composition according to Examples 1 to 6 were incubated for 24 hours, and then irradiated with UVB 80 mJ / cm 2 and measured by Multi-ModeMicroplateReader. It was.

As compared with Comparative Example 1 in which HaBaT cells were irradiated with UVB 80 mJ / cm 2,

When treated with the cosmetic composition according to Example 1 ROS was reduced by 78.4%,

When treated with the cosmetic composition according to Example 2 ROS is 78.4%,

When treated with the cosmetic composition according to Example 3 ROS is 76.4%,

When treated with the cosmetic composition according to Example 4 ROS is 76.9%,

When treated with the cosmetic composition according to Example 5 ROS is 77.5%,

When treated with the cosmetic composition according to Example 6, the ROS showed a decrease of 77.8% respectively. Through such a result, it was possible to confirm the active oxygen inhibitory effect of the cosmetic composition according to the present invention, and thus anti-aging effect of the manufactured cosmetics could be predicted.

[Test Example 6]

Look at the skin wound improvement effect of the lotion prepared according to Examples 7 to 12.

<Skin wound improvement effect>

After 6 m rat tails were each wounded to a size of 2 * 2 mm, the lotions prepared according to Examples 7 to 12 were applied and observed for 9 days every 3 days.

The result of skin wound improvement was expressed as a percentage of skin wound improvement in rat tail wound area using Scion image software.

After 3 days of application to the wound site of the rat tail for the lotion experiment prepared according to Examples 7 to 12, there was an improvement on skin wounds of an average of 22.15 ± 1.96%, on day 6 65.34 ± 4.80%, On the 9th day, the skin regeneration effect of 88.12 ± 2.45% was confirmed. As a result, it was confirmed that the skin wound improvement effect of the lotions prepared according to Examples 7 to 12 of the present invention was excellent.

The natural extract containing cosmetic composition having a skin wound improvement function according to the present invention and cosmetics comprising the same have excellent skin safety and excellent skin damage at the same time by using a natural extract, and antibacterial, antioxidant function additionally acts as a bacterium Due to the prevention of infection, such as the use of a cleaner and safer use has a great industrial applicability.

Claims (7)

After cutting green tea leaves, dry them in dry oven at 55-65 ℃ for 2-4 days, mix the dried green tea leaves with 70% ethanol in a 1:10 to 20 weight ratio, and then at 58-62 ℃. Green tea extract (a1) obtained by filtering the extract extracted for 3 to 5 hours with a filter paper and concentrating the filtrate under reduced pressure with a vacuum rotation evaporator for 5 to 7 hours under conditions of 0.1 to 0.3 mPA. 20.0 wt%;
Bamboo leaf and water were mixed at a weight ratio of 1: 15 to 30, then extracted at 100 ° C. for 15 to 30 hours, and the filtrate was filtered with filter paper for 5 to 7 hours under conditions of 0.1 to 0.3 mPA. 20.0 wt% of bamboo leaf extract (a2) obtained by decompression concentration with a vacuum rotation evaporator for a while;
After cutting the sesame leaves, the sesame leaves and 80% methanol is mixed at a weight ratio of 1: 10 to 15, and then extracted by standing for 20 to 30 hours, and the filtrate obtained by filtering the extract with a filter paper is 0.1 to 0.3 mPA. 15.0 wt% of sesame leaf extract (a3) obtained by concentrating under reduced pressure with a vacuum rotation evaporator for 5 to 7 hours under the condition of;
Gold and silver and purified water were mixed at a weight ratio of 1: 15 to 30, and then extracted by heating at 75 to 85 ° C. for 2 to 4 hours, and the filtrate was filtered with filter paper. 15.0 wt% of a sterling silver extract (a4) obtained by decompression concentration with a vacuum rotation evaporator for ˜7 hours;
After mixing steam and 80% methanol in a 1: 5 to 10 weight ratio, immersion extraction for 20 to 25 hours at 40 ~ 50 ℃, the extract was centrifuged at 11,000 ~ 13,000 rpm for 20 to 40 minutes in a centrifuge The extract was taken, and the supernatant was filtered using a filter paper, and the filtrate was concentrated under reduced pressure with a vacuum rotation evaporator for 5 to 7 hours under conditions of 0.1 to 0.3 mPA (a5) 15.0 wt%;
After mixing stone parsley and 80% methanol in a weight ratio of 1: 10 to 20, extracting at 70 to 80 ℃ for 2 to 4 hours, and extracting the filtered filtrate under reduced pressure for 5 to 7 hours under the conditions of 0.1 to 0.3 mPA. 20.0 in 100.0 wt.% of the first composition (A), which is composed of a mixture of (1) aa + a2 + a3 + a4 + a5 + a6; stone parsley extract (a6) obtained by concentrating under reduced pressure with a vacuum rotation evaporator. wt%,
Sea cucumber and ethanol are mixed at a weight ratio of 1:10 to 12, and then extracted at a shaker incubator at 120 to 160 rpm at 65 to 75 hours at 24 to 26 ° C, and the extracted extract is 15 to 25 at 7,500 to 8,500 rpm in a centrifuge. The supernatant obtained by centrifugation for minutes was filtered through filter paper, and the filtrate was concentrated under reduced pressure for 5 to 7 hours under a vacuum rotation evaporator under a condition of 0.1 to 0.3 mPA, and
After mixing gold and methanol in a 1: 4 to 6 weight ratio, the mixture was refluxed at 65 to 75 ° C for 20 to 30 hours, and the supernatant obtained by centrifuging the extract at 3,500 to 4,500 rpm for 4 to 6 minutes was filtered. Filtration with a paper (filter paper), the first mixture of the golden extract obtained by concentrating the filtered extract under reduced pressure for 5 to 7 hours under the conditions of 0.1 ~ 0.3 mPA in a vacuum rotation evaporator in the same weight ratio (b1) 20.0 wt%;
Flaxseed powder and ethanol were mixed at a weight ratio of 1: 10 to 15, then immersed at 50 to 80 ° C. for 2 to 10 hours, and the extracted extract was filtered through filter paper and the filtrate was 58 to 63 ° C. Flaxseed extract obtained by decompression concentration with a vacuum rotation evaporator for 5 to 7 hours at
After mixing the vitamin tree leaf and ethanol in a weight ratio of 1:15 to 25, and then immersing at room temperature for 22 to 26 hours to separate the supernatant and the precipitate, and repeating the same process three times the filter paper (filter paper) 20.0 wt% of a second mixed composition (b2), which was filtered and concentrated with a weight ratio of the vitamin tree leaf extract obtained by depressurizing the filtrate with a vacuum rotation evaporator at 58 to 63 ° C. for 5 to 7 hours;
After mixing black garlic and distilled water at a weight ratio of 1: 5 to 30, shaking extraction for 1 to 5 hours at 100 ° C. and 100 to 140 rpm, and extracting the filtered extract with a filter paper and filtering the filtrate to 0.1 Black garlic extract obtained by decompression concentration with a vacuum rotation evaporator for 5 to 7 hours under the condition of ˜0.3 mPA;
After mixing motherwort and distilled water at a weight ratio of 1: 5 to 30, shaking extraction for 1 to 5 hours at 100 ° C. and 100 to 140 rpm, and extracting the obtained extract at 2,000 to 4,000 rpm for 10 to 30 minutes in a centrifuge. After centrifugation, the mother liquor extract obtained by depressurizing the supernatant with a filter paper and depressurizing the filtrate with a vacuum rotation evaporator for 5 to 7 hours under the conditions of 0.1 to 0.3 mPA in the same weight ratio 20.0 wt% of the third mixed composition (b3), which was formulated with a mixture;
Persimmon leaf and ethanol were mixed at a weight ratio of 1: 5 to 30, and then reflux-cooled at 42 to 48 ° C. for 2 to 6 hours, and the extracted extract was centrifuged at 11,000 to 13,000 rpm for 20 to 40 minutes in an upper layer. The persimmon leaf extract obtained by extracting the filtrate and concentrating the filtrate with a filter paper and depressurizing the filtrate with a vacuum rotation evaporator for 5 to 7 hours under conditions of 0.1 to 0.3 mPA;
The riding flower and 70% ethanol were mixed at a weight ratio of 1: 5 to 30, shaken at 23 to 28 ° C. and 80 to 150 rpm for 40 to 55 hours, and the obtained extract was filtered with a filter paper. 20.0 wt% of a fourth mixed composition (b4) in which the filtrate was concentrated under reduced pressure with a vacuum rotation evaporator for 5 to 7 hours under a condition of 0.1 to 0.3 mPA in the same weight ratio;
After mixing chestnut leaves and ethanol at a weight ratio of 1: 10 to 15, and then immersing at room temperature for 22 to 26 hours to separate supernatant and precipitate, and repeating the same process three times and filtering the extracted extract with filter paper. Chestnut leaf extract obtained by concentrating the filtrate under reduced pressure with a vacuum rotation evaporator at 58-63 ° C. for 5-7 hours.
After mixing 1 to 20-22 weight ratio of panacea and ethanol, and immersed for 7 to 8 days at room temperature, the supernatant is separated and filtered three times, and 5 at the conditions of 0.1 ~ 0.3 mPA To a mixture of (b1 + b2 + b3 + b4 + b5); the fifth mixed composition (b5) 20.0 wt%; Cosmetic composition having a skin wound improvement function, characterized in that the cosmetic composition consisting of (A + B) of 80.0 wt% in the composition 100.0 wt% of the second composition (B).
95.0 wt% of the cosmetic composition of claim 1,
After mixing the dry solid and 70% ethanol in a weight ratio of 1: 10 to 20, shaking extract for 40 to 50 hours at a condition of 80 ~ 150 rpm, 23 ~ 28 ℃ in a shake extractor, extract extract filter paper A cosmetic composition having a skin wound improvement function, characterized in that the filtrate is composed of 5.0 wt% of the extract obtained by distilling the filtrate under reduced pressure with a vacuum rotation evaporator at 58 to 63 ° C. for 5 to 7 hours.
95.0 wt% of the cosmetic composition of claim 1,
Dry powdered gardenia berry powder and distilled water were mixed at a weight ratio of 20 to 40, and then refluxed and extracted at 100 ° C. for 20 to 40 minutes by a heating circulation extraction method, and the extract was filtered by filter paper, Cosmetic composition having a skin wound improvement function, characterized in that the filtrate is composed of 5.0 wt% of the gardenia extract obtained by depressurizing the filtrate with a vacuum rotation evaporator for 5 to 7 hours at 58 ~ 63 ℃.
95.0 wt% of the cosmetic composition of claim 1,
After mixing Angelica and 70% ethanol in a weight ratio of 1: 10 to 15, immersion extraction for 20 to 30 hours at 55 ~ 65 ℃, the extract was filtered by filter paper, and the filtrate at 58 ~ 63 ℃ 5 Cosmetic composition having a skin wound improvement function, characterized in that consisting of 5.0 wt% extract obtained by decompression concentration with a vacuum rotation evaporator for 7 hours.
95.0 wt% of the cosmetic composition of claim 1,
Peppermint and 80% ethanol were mixed at a weight ratio of 1: 10 to 20, and then immersed and extracted at 55 to 65 ° C. for 20 to 30 hours, the extract was filtered with filter paper, and the filtrate was washed at 58 to 63 ° C. 5. Cosmetic composition having a skin wound improvement function, characterized in that consisting of 5.0 wt% peppermint extract obtained by decompression concentration with a vacuum rotation evaporator for ~ 7 hours.
98.0 wt% of the cosmetic composition of claim 1,
After mixing acid and methanol in a weight ratio of 10 to 15, the mixture was extracted with stirring at room temperature for 22 to 26 hours, the extracted extract was filtered through a filter paper, and the filtrate was filtered under the conditions of 0.1 to 0.3 mPA. 25.0 wt% of an extract extracted from the extract under reduced pressure with a vacuum rotation evaporator for ˜7 hours;
The moss was washed with water, dried at 55-65 ° C., hot air dried and pulverized. The mixed moss and 100% methanol were mixed at a weight ratio of 1: 10 to 15, and then refluxed with a cooling tube at 58-62 ° C. 5. Moss obtained by reflux cooling for 7 hours, the extracted extract was filtered through a filter paper, and the filtrate was concentrated under reduced pressure with a vacuum rotation evaporator for 5 to 7 hours under the conditions of 0.1 to 0.3 mPA. Extract 25.0 wt%;
After washing with baekduong, dried with lyophilizer, processed into powder (powder), mixed with powdery baekduong and distilled water at a weight ratio of 1: 10 ~ 12 times and reflux extraction at 75 ~ 85 ℃ for 2 ~ 4 hours And, the extracted extract was filtered with a filter paper (filter paper), 25.0 wt% Baekduong extract obtained by concentrating the filtrate under reduced pressure with a vacuum rotation evaporator for 5 to 7 hours under the conditions of 0.1 ~ 0.3 mPA;
After mixing grapefruit seed and ethanol at a weight ratio of 1: 5 to 10, reflux cooling for 2 to 3 hours at a temperature of 60 to 62 ℃, and filter the extracted extract with a filter paper, the filtrate 10,000 The supernatant taken by centrifugation at × g for 20 to 40 minutes was concentrated under reduced pressure for 5 to 7 hours in a vacuum rotation evaporator under a condition of 0.1 to 0.3 mPA; 25.0 wt% of grapefruit seed extract; Cosmetic composition having a skin wound improvement function, characterized in that consisting of 2.0 wt% in 100.0 wt% of the preservation functional composition.
A cosmetic having a skin wound improvement function, which is prepared by adding the cosmetic composition of any one of claims 1 to 6 at 5.0 wt% based on the total weight of the lotion.