KR101988489B1 - Functional cosmetic composition for anti-dust and anti-inflamatory containing natural extract as an effective ingredient and functional cosmetic including the same - Google Patents

Abstract

The present invention relates to a cosmetic composition comprising natural plant extracts as active ingredients for preventing fine dusts from penetrating the skin and suppressing inflammations; and a functional cosmetic product comprising the same. In particular, the cosmetic composition comprises a mixture of a first composition and a second composition. In particular, the first composition comprises, in a predetermined mixing ratio, a first mixture composition, a second mixture composition, a third mixture composition, a fourth mixture composition, and a fifth mixture composition, wherein the first mixture composition contains an equal weight of sea cucumber extracts and spirulina extracts, the second mixture composition contains an equal weight of flaxseed extracts and rapeseed meal extracts, the third mixture composition contains an equal weight of dark garlic extracts and lotus root extracts, the fourth mixture composition contains an equal weight of persimmon leaf extracts and Smilacis Rhizoma extracts, and the fifth mixture composition contains an equal weight of chestnut leaf extracts and acacia flower extracts. The second composition comprises, in a predetermined mixing ratio: Opuntia humifusa stem extracts; Sophora flavescens extracts; seaweed extracts; hijiki extracts; and jujube seed extracts. The first composition provides benefits in terms of moisturization, antioxidant effects, skin protection, wrinkle reduction, skin-brightening effects, and anti-aging effects, while the second composition provides benefits in terms of preventing fine dusts from penetrating the skin and suppressing skin inflammations.

Description

TECHNICAL FIELD [0001] The present invention relates to a cosmetic composition comprising a natural extract as an active ingredient and having a function of inhibiting skin infiltration of a fine dust and inhibiting skin inflammation, and a functional cosmetic using the same. BACKGROUND ART COSMETIC INCLUDING THE SAME}

The present invention relates to a first mixed composition comprising a combination of sea cucumber extract and spirulina extract at a same weight ratio; A flaxseed extract, and a hybridoma extract in a weight ratio; Black garlic extract, and rootstock extract were compounded in the same weight ratio; A persimmon leaf extract and a tobacco root extract; A chestnut leaf extract and an acacia flower extract in a weight ratio of 1: 1,

Opuntia humifusa stem extract; Ginseng extract; Kelp extract; Top extract%; Antioxidation, skin protection and wrinkle improvement, whitening, anti-aging function by the first composition, and fine-grained by the second composition can be obtained by mixing the first composition and the second composition, And a functional cosmetic using the natural botanical extract as an active ingredient. The present invention also relates to a cosmetic composition containing the natural botanical extract as an active ingredient.

Particulate matter floating in the air is called dust, and particles of a size that can not be recognized by the naked eye are called fine dust particles. Fine dust is classified according to the size of the particles, and it is named as PM 10, PM 2.5, and PM 1 according to their size, which means the particle size unit of the atmospheric particles.

PM refers to the fine dust that we commonly know as particles with a particle size of 10 μm (micrometer) or less. PM 2.5 is the size of ultra fine dust and has a particle size of 2.5 μm (micrometer) or less. Ultrafine dust PM 1 refers to the concentration of particles having a size of 1 μm (micrometer) or less. The reason why the unit of the range is gradually lowered from PM 10 to PM 1 is because the particle size is smaller and harmful to human body and environment.

The risk of ultrafine dusts containing heavy metals such as lead, cadmium and various chemical substances weakens the metabolism flow of skin and lowers the function of control of sebum and causes dryness, itching, and skin trouble, and heavy metals contained in fine dust Harmful substances such as environmental hormones stimulate the secretion of inflammatory cytokines from the cells and increase the number of white blood cells, resulting in allergic reactions to atopic dermatitis. When the fine dust is absorbed into the skin, it also stimulates the pigment cells to form wrinkles and black spots. When the fine dust is absorbed into the body, it creates a toxic substance called active oxygen, and this active oxygen breaks down the collagen in the skin, thereby reducing the elasticity of the skin, resulting in exogenous aging.

In particular, when fine dust comes into direct contact with the skin located on the outermost part of the body, the skin acts as a barrier between the organism and the environment. If the skin is directly exposed to frequent contamination, Causing problems. Particularly fine dust is 20 times smaller than pores, so it can easily penetrate into human skin. For example, absorption of substances such as chloroform, a toxic substance, into the skin is considered to be the same as inhalation by the respiratory tract, which is the main site for absorbing substances from environmental pollution.

In connection with the present invention, a cosmetic composition for prevention of infiltration of fine dusts and inhibition of inflammation reaction containing 'a natural plant extract as an active ingredient' of Korean Patent No. 10-1817109 (Registration Date 2018.01.04); A technique for skin irritation and skin irritation mitigation by fine dust containing an extract of holly basil, mint, and mung bean as an active ingredient in Korean Patent Laid-Open Publication No. 10-2018-0014356 (Publication date 2018.02.08) There is a bar.

Conventionally disclosed cosmetics or cosmetics are disadvantageous in that they are not limited to having a complex function of moisturizing, antioxidation, skin protection and wrinkle improvement, whitening and antiaging, at the same time as inhibiting skin penetration and skin inflammation of fine dusts.

Korean Registered Patent No. 10-1817109 (Registration date 2018.01.04) Korean Patent Publication No. 10-2018-0014356 (published date 2018.02.08)

The present invention relates to a first mixed composition comprising a combination of sea cucumber extract and spirulina extract at a same weight ratio; A flaxseed extract, and a hybridoma extract in a weight ratio; Black garlic extract, and rootstock extract were compounded in the same weight ratio; A persimmon leaf extract and a tobacco root extract; A chestnut leaf extract and an acacia flower extract in a weight ratio of 1: 1,

Opuntia humifusa stem extract; Ginseng extract; Kelp extract; Top extract%; Antioxidation, skin protection and wrinkle improvement, whitening, anti-aging function by the first composition, and fine-grained by the second composition can be obtained by mixing the first composition and the second composition, A cosmetic composition containing a natural plant extract as an active ingredient which enables the skin to remain in a healthy state by acting in combination with a function of blocking penetration of dust into the skin and suppressing the development of skin inflammation and a functional cosmetic using the cosmetic composition. .

To achieve the above objects,

In the present invention, the sea cucumber and ethanol are mixed at a weight ratio of 1:10 to 12, and the mixture is extracted from the shaker culture at 120 to 160 rpm at 24 to 26 ° C for 65 to 75 hours. The extracted extract is centrifuged at 7,500 to 8,500 rpm The supernatant obtained by centrifugation for 15 to 25 minutes was filtered with a filter paper, and the filtrate was concentrated under reduced pressure for 5 to 7 hours under a condition of 0.1 to 0.3 mPA using a vacuum rotation evaporator Sea cucumber extract,

Spirulina and distilled water were mixed at a weight ratio of 1: 5 to 30, and the mixture was shaken in an incubator at 20 to 30 ° C and 130 to 160 rpm for 50 to 80 hours. The extracted extract was centrifuged at 7,000 to 9,000 rpm for 10 The supernatant obtained by centrifugation for 30 minutes was filtered with a filter paper, and the filtrate was vacuum-evaporated using a vacuum rotation evaporator at 0.1 to 0.3 mPa for 5 to 7 hours. 0.5 to 60.0 wt% of the first mixed composition (a1) prepared by mixing the spirulina extract obtained by concentrating under reduced pressure with the above mixture at a same weight ratio;

Flaxseed powder and ethanol are mixed at a weight ratio of 1:10 to 15, and then the mixture is dipped at 50 to 80 ° C for 2 to 10 hours to extract. The extracted extract is filtered with a filter paper, For 5 to 7 hours under reduced pressure using a vacuum rotation evaporator,

After mixing the seeds and distilled water at a weight ratio of 1: 1, the mixture is heated and extracted at 100 ° C for 1 to 3 hours. The extracted extract is filtered with a filter paper, and the filtrate is stirred at 75 to 85 ° C for 5 to 7 hours 0.5 to 60.0 wt% of a second mixed composition (a2) prepared by blending the seed oil extracts obtained by concentration under reduced pressure with a vacuum rotation evaporator in the same weight ratio;

The mixture of black garlic and distilled water was mixed at a weight ratio of 1: 5 to 30, and the mixture was shaken for 1 to 5 hours under the conditions of 100 ° C and 100 to 140 rpm. The extracted extract was filtered with a filter paper, The extract of black garlic obtained by concentration under reduced pressure with a vacuum rotation evaporator for 5 to 7 hours under the condition of ~ 0.3 mPA,

After mixing the dried rootstock powder with ethanol at a weight ratio of 1: 5 to 30, the mixture is immersed at room temperature for 20 to 30 hours to extract the extract. The extracted extract is filtered with filter paper, and the filtrate is filtered under conditions of 0.1 to 0.3 mPa 0.5 to 60.0 wt% of a third mixed composition (a3) prepared by blending the extracts of L. roots obtained by concentration under reduced pressure with a vacuum rotation evaporator for 5 to 7 hours in the same weight ratio;

The mixture of persimmon leaves and ethanol was mixed at a weight ratio of 1: 5 ~ 30, and the mixture was reflux cooled for 2 ~ 6 hours at 42 ~ 48 ℃. The extract was centrifuged at 11,000 ~ 13,000 rpm for 20 ~ The supernatant was filtered with a filter paper, and the filtrate was concentrated under reduced pressure using a vacuum rotation evaporator for 5 to 7 hours under the condition of 0.1 to 0.3 mPA.

The mixture of soil and ethanol was mixed at a weight ratio of 1: 5 ~ 30, and the mixture was immersed at 50 ~ 80 ℃ for 2 ~ 10 hours. The extracted extract was filtered with a filter paper to obtain a filtrate of 0.1 ~ 0.3 mPA 0.5 to 60.0 wt% of a fourth mixed composition (a4) in which the soil extract obtained by concentration under reduced pressure with a vacuum rotation evaporator for 5 to 7 hours is mixed in the weight ratio;

Chestnut leaf and ethanol were mixed at a weight ratio of 1:10 to 15, and then the mixture was immersed at room temperature for 22 to 26 hours to separate the supernatant and the precipitate. The same procedure was repeated three times, and the extracted extract was filtered with a filter paper , Chestnut leaf extract obtained by concentrating the filtrate under reduced pressure at 58 to 63 ° C for 5 to 7 hours with a vacuum rotation evaporator,

After drying and finishing the Acacia (Robiniapseudoacacia) flower, the powder is mixed with the purified water at a weight ratio of 1:35 to 45, and then extracted at 85 to 99 ° C for 5 to 7 hours. The extracted extract is filtered with filter paper , And the filtrate was centrifuged at 2,000 ~ 4,000 rpm for 10 ~ 30 minutes in a centrifuge. The supernatant was filtered and the filtrate was concentrated under reduced pressure using a vacuum rotation evaporator for 5 to 7 hours under the condition of 0.1 to 0.3 mPA (A1 + a2 + a3 + a4 + a5) of 0.5 to 60.0 wt% of a fifth mixed composition (a5) prepared by mixing the obtained acacia flower extract in a weight ratio of 100.0 wt% ) 50.0 to 95.0 wt%

The Opuntiahumifusa stems were dried at room temperature for 20 to 30 hours. The pulverized material was mixed with 80% ethanol at a weight ratio of 1: 2 to 5, and then the mixture was extracted at room temperature for 20 to 30 hours for extraction. Was filtered through a filter paper, and the filtrate was centrifuged at 2,000-4,000 rpm for 10-30 minutes in a centrifugal separator. The supernatant was filtered and the filtrate was filtrated for 5 to 7 hours at 0.1 to 0.3 mPa 1.5 to 45.5 wt% of Opuntia humifusa stem extract (b1) obtained by concentration under reduced pressure with a vacuum rotation evaporator;

The extract was filtered through a filter paper, and the filtrate was filtered to remove the filtrate. The filtrate was filtered through a filter paper, 1.5 to 45.5 wt% of the extract (b2) obtained by concentration under reduced pressure with a vacuum rotation evaporator for 5 to 7 hours under the condition of 0.3 mPA;

The kelp was dried in a hot air dryer at 58 to 62 ° C for 10 to 14 hours, and the pulverized dried powder and distilled water were mixed at a weight ratio of 1:15 to 20, and the mixture was heated and extracted at 80 to 90 ° C for 1 to 3 hours. 1.5 to 45.5 wt% of an extract of sea tangle (b3) obtained by concentrating the extract with a vacuum rotation evaporator under the condition of 0.1 to 0.3 mPA for 5 to 7 hours under reduced pressure;

The dried powder was dried at 58 to 62 ° C in a hot air drier for 10 to 14 hours. The pulverized dried powder and distilled water were mixed at a weight ratio of 1:15 to 20, and the mixture was heated and extracted at 80 to 90 ° C for 1 to 3 hours. 1.5 to 45.5 wt% of the extract (b4) obtained by concentrating the extract with a vacuum rotation evaporator under the condition of 0.1 to 0.3 mPA for 5 to 7 hours under reduced pressure;

The jujube seeds were mixed with 80% methanol at a ratio of 1: 8 ~ 12 by weight and then extracted at room temperature for 20 ~ 30 hours. The extracted extract was filtered with a filter paper, The filtrate was centrifuged at 2,000 to 4,000 rpm for 10 to 30 minutes in a centrifuge, and the supernatant was filtered. The filtrate was concentrated under reduced pressure using a vacuum rotation evaporator for 5 to 7 hours at 0.1 to 0.3 mPa (A +) of 100.0 wt% of the second composition (B) composed of the mixture (b1 + b2 + b3 + b4 + b5) of 1.5 to 45.5 wt% B). The cosmetic composition according to the present invention comprises a natural extract as an active ingredient, and has a function of blocking penetration of fine dusts and inhibiting skin inflammation.

Also, the functional cosmetic composition is prepared by adding the cosmetic composition at 0.1 to 50.0 wt% with respect to the total weight of the cosmetic.

The cosmetic composition comprising the natural extract according to the present invention as an active ingredient and having a function of inhibiting skin infiltration of the fine dust and inhibiting skin inflammation, and a functional cosmetic using the cosmetic composition have the following effects.

first. A sea mixture, a sea cucumber extract and a spirulina extract at a same weight ratio; A flaxseed extract, and a hybridoma extract in a weight ratio; Black garlic extract, and rootstock extract were compounded in the same weight ratio; A persimmon leaf extract and a tobacco root extract; Chestnut leaf extract, and acacia flower extract in a weight ratio of 1: 1 to 5: 1 by weight of the first composition, wherein the first composition is mixed and formed at a predetermined blending ratio to provide moisturizing, antioxidant, skin protection and wrinkle improvement, whitening and antiaging effect.

second. Opuntia humifusa stem extract; Ginseng extract; Kelp extract; Top extract%; The composition of the present invention has a function of preventing skin infiltration of fine dusts and inhibiting skin inflammation by mixing the composition with a composition containing a jujube extract at a certain mixing ratio.

third. By using the natural extract, it is possible to provide a complex function useful for skin by the first composition and the second composition which constitute the cosmetic composition and which is free of skin irritation and stable to the skin.

fourth. Acanthopanax extract; Moss extract; Baechutong extract; The addition of the third composition comprising the grapefruit seed extract to the cosmetic composition provides antimicrobial and antioxidant functionalities and thus can be used more cleanly and safely due to blocking of the bacterial infection and can prolong the preservation period Respectively.

Hereinafter, the technical construction according to the present invention will be described in more detail.

As described above, the cosmetic composition comprising the natural extract according to the present invention as an active ingredient and having a function of inhibiting skin infiltration of the fine dust and inhibiting skin inflammation,

The mixture of sea cucumber and ethanol was mixed at a weight ratio of 1:10 to 12, and the mixture was extracted with shaking incubator at 120 to 160 rpm at 24 to 26 ° C for 65 to 75 hours. The extracted extract was centrifuged at 7,500 to 8,500 rpm for 15 to 25 Min. The supernatant obtained by centrifuging was filtered with a filter paper, and the filtrate was concentrated under reduced pressure for 5 to 7 hours under a condition of 0.1 to 0.3 mPa by a vacuum rotation evaporator.

Spirulina and distilled water were mixed at a weight ratio of 1: 5 to 30, and the mixture was shaken in an incubator at 20 to 30 ° C and 130 to 160 rpm for 50 to 80 hours. The extracted extract was centrifuged at 7,000 to 9,000 rpm for 10 The supernatant obtained by centrifugation for 30 minutes was filtered with a filter paper, and the filtrate was vacuum-evaporated using a vacuum rotation evaporator at 0.1 to 0.3 mPa for 5 to 7 hours. 0.5 to 60.0 wt% of the first mixed composition (a1) prepared by mixing the spirulina extract obtained by concentrating under reduced pressure with the above mixture at a same weight ratio;

Flaxseed powder and ethanol are mixed at a weight ratio of 1:10 to 15, and then the mixture is dipped at 50 to 80 ° C for 2 to 10 hours to extract. The extracted extract is filtered with a filter paper, For 5 to 7 hours under reduced pressure using a vacuum rotation evaporator,

After mixing the seeds and distilled water at a weight ratio of 1: 1, the mixture is heated and extracted at 100 ° C for 1 to 3 hours. The extracted extract is filtered with a filter paper, and the filtrate is stirred at 75 to 85 ° C for 5 to 7 hours 0.5 to 60.0 wt% of a second mixed composition (a2) prepared by blending the seed oil extracts obtained by concentration under reduced pressure with a vacuum rotation evaporator in the same weight ratio;

The mixture of black garlic and distilled water was mixed at a weight ratio of 1: 5 to 30, and the mixture was shaken for 1 to 5 hours under the conditions of 100 ° C and 100 to 140 rpm. The extracted extract was filtered with a filter paper, The extract of black garlic obtained by concentration under reduced pressure with a vacuum rotation evaporator for 5 to 7 hours under the condition of ~ 0.3 mPA,

After mixing the dried rootstock powder with ethanol at a weight ratio of 1: 5 to 30, the mixture is immersed at room temperature for 20 to 30 hours to extract the extract. The extracted extract is filtered with filter paper, and the filtrate is filtered under conditions of 0.1 to 0.3 mPa 0.5 to 60.0 wt% of a third mixed composition (a3) prepared by blending the extracts of L. roots obtained by concentration under reduced pressure with a vacuum rotation evaporator for 5 to 7 hours in the same weight ratio;

The mixture of persimmon leaves and ethanol was mixed at a weight ratio of 1: 5 ~ 30, and the mixture was reflux cooled for 2 ~ 6 hours at 42 ~ 48 ℃. The extract was centrifuged at 11,000 ~ 13,000 rpm for 20 ~ The supernatant was filtered with a filter paper, and the filtrate was concentrated under reduced pressure using a vacuum rotation evaporator for 5 to 7 hours under the condition of 0.1 to 0.3 mPA.

The mixture of soil and ethanol was mixed at a weight ratio of 1: 5 ~ 30, and the mixture was immersed at 50 ~ 80 ℃ for 2 ~ 10 hours. The extracted extract was filtered with a filter paper to obtain a filtrate of 0.1 ~ 0.3 mPA 0.5 to 60.0 wt% of a fourth mixed composition (a4) in which the soil extract obtained by concentration under reduced pressure with a vacuum rotation evaporator for 5 to 7 hours is mixed in the weight ratio;

Chestnut leaf and ethanol were mixed at a weight ratio of 1:10 to 15, and then the mixture was immersed at room temperature for 22 to 26 hours to separate the supernatant and the precipitate. The same procedure was repeated three times, and the extracted extract was filtered with a filter paper , Chestnut leaf extract obtained by concentrating the filtrate under reduced pressure at 58 to 63 ° C for 5 to 7 hours with a vacuum rotation evaporator,

After drying and finishing the Acacia (Robiniapseudoacacia) flower, the powder is mixed with the purified water at a weight ratio of 1:35 to 45, and then extracted at 85 to 99 ° C for 5 to 7 hours. The extracted extract is filtered with filter paper , And the filtrate was centrifuged at 2,000 ~ 4,000 rpm for 10 ~ 30 minutes in a centrifuge. The supernatant was filtered and the filtrate was concentrated under reduced pressure using a vacuum rotation evaporator for 5 to 7 hours under the condition of 0.1 to 0.3 mPA (A1 + a2 + a3 + a4 + a5) of 0.5 to 60.0 wt% of a fifth mixed composition (a5) prepared by mixing the obtained acacia flower extract in a weight ratio of 100.0 wt% ) 50.0 to 95.0 wt%

The Opuntiahumifusa stems were dried at room temperature for 20 to 30 hours. The pulverized material was mixed with 80% ethanol at a weight ratio of 1: 2 to 5, and then the mixture was extracted at room temperature for 20 to 30 hours for extraction. Was filtered through a filter paper, and the filtrate was centrifuged at 2,000-4,000 rpm for 10-30 minutes in a centrifugal separator. The supernatant was filtered and the filtrate was filtrated for 5 to 7 hours at 0.1 to 0.3 mPa 1.5 to 45.5 wt% of Opuntia humifusa stem extract (b1) obtained by concentration under reduced pressure with a vacuum rotation evaporator;

The extract was filtered through a filter paper, and the filtrate was filtered to remove the filtrate. The filtrate was filtered through a filter paper, 1.5 to 45.5 wt% of the extract (b2) obtained by concentration under reduced pressure with a vacuum rotation evaporator for 5 to 7 hours under the condition of 0.3 mPA;

The kelp was dried in a hot air dryer at 58 to 62 ° C for 10 to 14 hours, and the pulverized dried powder and distilled water were mixed at a weight ratio of 1:15 to 20, and the mixture was heated and extracted at 80 to 90 ° C for 1 to 3 hours. 1.5 to 45.5 wt% of an extract of sea tangle (b3) obtained by concentrating the extract with a vacuum rotation evaporator under the condition of 0.1 to 0.3 mPA for 5 to 7 hours under reduced pressure;

The dried powder was dried at 58 to 62 ° C in a hot air drier for 10 to 14 hours. The pulverized dried powder and distilled water were mixed at a weight ratio of 1:15 to 20, and the mixture was heated and extracted at 80 to 90 ° C for 1 to 3 hours. 1.5 to 45.5 wt% of the extract (b4) obtained by concentrating the extract with a vacuum rotation evaporator under the condition of 0.1 to 0.3 mPA for 5 to 7 hours under reduced pressure;

The jujube seeds were mixed with 80% methanol at a ratio of 1: 8 ~ 12 by weight and then extracted at room temperature for 20 ~ 30 hours. The extracted extract was filtered with a filter paper, The filtrate was centrifuged at 2,000 to 4,000 rpm for 10 to 30 minutes in a centrifuge, and the supernatant was filtered. The filtrate was concentrated under reduced pressure using a vacuum rotation evaporator for 5 to 7 hours at 0.1 to 0.3 mPa (A +) of 100.0 wt% of the second composition (B) composed of the mixture (b1 + b2 + b3 + b4 + b5) of 1.5 to 45.5 wt% B).

The cosmetic composition according to the present invention comprises a first mixed composition comprising a mixture of a sea cucumber extract and a spirulina extract in the same weight ratio; A flaxseed extract, and a hybridoma extract in a weight ratio; Black garlic extract, and rootstock extract were compounded in the same weight ratio; A persimmon leaf extract and a tobacco root extract; A chestnut leaf extract and an acacia flower extract in a weight ratio of 1: 1,

Opuntia humifusa stem extract; Ginseng extract; Kelp extract; Top extract%; It is a functional cosmetic composition in which the penetration of the skin of the fine dust is inhibited and the function of inhibiting the skin inflammation is combined.

≪ First composition >

Wherein the first composition comprises 0.5 to 60.0 wt% of a first mixed composition comprising a mixture of a sea cucumber extract and a spirulina extract in the same weight ratio; 0.5 to 60.0 wt% of a second mixed composition prepared by mixing the flaxseed extract and the seedling oil extract in the same weight ratio; Black garlic extract, and rootstock extract in a weight ratio of 0.5 to 60.0 wt%; A persimmon leaf extract, and a tobacco root extract in a weight ratio of 0.5 to 60.0 wt%; Chestnut leaf extract and acacia flower extract in a weight ratio of 0.5 to 60.0 wt%; and a specific formulation thereof is shown in Table 1 below.

Formulation Example 1 Formulation Example 2 Formulation Example 3 Formulation Example 4 Formulation Example 5 The first mixed composition 10.0 wt% 25.0 wt% 15.0 wt% 30.0 wt% 20.0 wt% The second mixed composition 20.0 wt% 10.0 wt% 25.0 wt% 15.0 wt% 20.0 wt% Third mixed composition 30.0 wt% 20.0 wt% 10.0 wt% 25.0 wt% 20.0 wt% Fourth mixed composition 15.0 wt% 30.0 wt% 20.0 wt% 10.0 wt% 20.0 wt% Fifth mixed composition 25.0 wt% 15.0 wt% 30.0 wt% 20.0 wt% 20.0 wt% Sum 100.0 wt% 100.0 wt% 100.0 wt% 100.0 wt% 100.0 wt%

1. First mixed composition

The first mixed composition has a moisturizing function, and is prepared by mixing the sea cucumber extract and the spirulina extract at a weight ratio of 1: 1.

Sea cucumber is a generic term for sea cucumbers belonging to the sea cucumber river. There are three kinds of sea cucumbers in Korea, red ginseng, blue ginseng and black ginseng. Sea cucumbers act as amino acid and iron (Fe), phosphorus (P) and bile components such as cysteine, histidine and lysine to prevent anemia and liver. Taurine (Taurine) is contained in the gallbladder and liver to detoxify the function of strengthening, cholesterol lowering, heart function and improve vision is said to be effective in the recovery.

The raw sea cucumber contains water, protein, carbohydrate, ash, calcium, phosphorus, iron, lysine, vitamin 1, B2, essential amino acid, erythrocyte thionin, glutamic acid, glycine, tryptophan and taurine. Sea cucumber extracts from sea cucumbers contain chondroitin, a type of mucopolysaccharide. Chondroitin plays a role in accumulating moisture and nutrients in the skin. Chondroitin sulfate is a major component of viscous polysaccharides and is a mucopolysaccharide found in animal cartilage, nervous tissue and intercellular tissue. Chondroitin sulfate does not exist in vivo but is bound to proteins to form a chondroitin sulfate protein complex. It is the basis for living somatic cells in vivo, such as collagen, elastin, hyaluronic acid, etc., It gives resilience. Chondroitin sulfate, which is contained in sea cucumber extract, keeps moisturizing function by storing moisture in the skin. It also stores a large amount of amino acid, protein and iodine ingredient to save skin wrinkles, prevent aging, skin whitening efficacy, .

The sea cucumber extracts were prepared by mixing sea cucumber and ethanol at a weight ratio of 1:10 to 12, and then extracting the extracts at 24 to 26 ° C for 65 to 75 hours at 120 to 160 rpm in a shaking incubator. The extracted extracts were centrifuged at 7,500 to 8,500 rpm The supernatant obtained by centrifugation for 15 to 25 minutes was filtered with a filter paper, and the filtrate was concentrated under reduced pressure for 5 to 7 hours under a condition of 0.1 to 0.3 mPA using a vacuum rotation evaporator .

As a specific example of the above-mentioned sea cucumber extract, 1,000 g of sea cucumber is placed in 10 L of 50% ethanol and extracted at 160 rpm at 26 DEG C for 70 hours in a shaking incubator to obtain an extract. The extract is centrifuged at 7,000 rpm for 30 minutes in a centrifuge. The supernatant obtained by centrifugation is filtered with filter paper (Whatman No. 3 filter paper). The filtered extract was concentrated under reduced pressure at 62 DEG C and 0.2 mPA for 5 hours by a vacuum rotation evaporator to obtain 1 L of an extract.

Spirulina (Spirulinaplatensis) is one of the oldest algae on Earth and is the first photosynthetic creature born about 3.5 billion years ago.

Ingredients include 60-70% protein, 6-9% lipid, carbohydrate 15-20% carbohydrate and large amounts of vitamins, minerals and fiber. Among the lipid components, free-fatty acid accounts for 70 ~ 80%, which is composed of linoleic acid and γ-linolenic acid.

Carbohydrates include glucose, rhamnose, mannose, and xylose. Coloring agents include light yellow carotenoids, green chlorophyll, and blue phycocyanin. It also contains a large amount of vitamin B12 and phenolic acid, tocopherols, and β-carotene, which act as antioxidants.

The spirulina extract is mixed with spirulina and distilled water at a weight ratio of 1: 5 to 30, and then shaken out in an incubator at 20 to 30 ° C and 130 to 160 rpm for 50 to 80 hours. The extracted extract is centrifuged at 7,000 ~ The supernatant obtained by centrifugation at 9,000 rpm for 10 to 30 minutes was filtered with a filter paper, and the filtrate was concentrated under reduced pressure in a vacuum concentrator (0.1-0.3 mPA) for 5-7 hours, (vacuum rotation evaporator).

More specifically, 1,000 g of Spirulina was added to 25 L of distilled water, and the mixture was shaken for extraction at 25 DEG C and 150 rpm in an incubator for 70 hours. The extracted extract is centrifuged at 8,500 rpm for 20 minutes in a centrifuge. The supernatant was collected by filtration through filter paper (Whatman No. 2), and the filtrate was concentrated under reduced pressure at 0.2 mPA for 5 hours using a vacuum rotation evaporator to obtain 1 L of an extract.

2. Second Mixture Composition

The second mixed composition has an antioxidant function, and is mixed and blended at a ratio of 1: 1 by weight of the flaxseed extract and the seed oil extract.

The flaxseed is Linum usitatissimum and is a seed of the flax (Linaceae) plant. Flaxseed contains vitamins and essential amino acids. However, there are three representative beneficial ingredients that are rich in omega-3 fatty acids, lignans and dietary fiber.

Lignans have antioxidant properties and have very useful antioxidant activities in human body. It has excellent anticancer, anti-cleavage, and anti-toxicity and has a function of strongly inhibiting already formed tumors.

The flaxseed extract is prepared by mixing the flaxseed powder with ethanol at a weight ratio of 1:10 to 15, and then dipping the mixture at 50 to 80 ° C for 2 to 10 hours. The extracted extract is filtered with a filter paper, And obtained by concentrating under reduced pressure with a vacuum rotation evaporator at 58 to 63 ° C for 5 to 7 hours.

Specific examples of the flaxseed extract include,

After drying, 1,000 g of the pulverized flaxseed powder is immersed in 10 L of 95% ethanol, and then heated at 62 DEG C for 3 hours. The extract was filtered with a filter paper, and the filtered extract was concentrated under reduced pressure at 62 DEG C and 0.2 mPA for 5 hours using a vacuum rotation evaporator to obtain 1 L of an extract.

It is a byproduct of the process of producing rapeseed oil from rapeseed. The seeds have higher protein and carbohydrate contents than the seeds and are not cytotoxic. And has excellent antioxidant activity.

The seeds of the seedlings were mixed with distilled water and distilled water at a weight ratio of 1: 1, followed by heating at 100 ° C for 1 to 3 hours. The extracted extract was filtered with a filter paper, And concentrated under reduced pressure using a vacuum rotation evaporator for 5 to 7 hours.

Specific examples of the plant extracts include,

1,000 g of the seedlings and 1 L of distilled water are mixed and then heated at 100 ° C. for 2 hours. The extract was filtered with a filter paper, and the filtrate was concentrated under reduced pressure at 80 DEG C and 0.2 mPA for 5 hours to obtain 0.5 L of an extract.

3. Third Mixture Composition

The third mixed composition has a skin protection and wrinkle-improving function, and is mixed and mixed at a weight ratio of 1: 1 of black garlic extract and rootstock extract.

When garlic is aged in a high-temperature thermostat for a certain period of time, garlic is changed into black by its own components and enzymes, etc., and this is called "black garlic".

As the raw garlic turns into black garlic, the unstable and odorous components of raw garlic are changed into a more stable and odorless water-soluble substance and organic sulfur compounds such as S-allylcysteine (SAC), S-allylmercaptocystein (SAMC) and tetrahydro- soluble substances such as diallyl sulfide (DAS), triallyl sulfide, diallyl disulfide (DADS) and diallyl polysulfides. Fructosyl-arginine inhibits the production of thiobarbituric acid reactive substances (TBARS) in the liver and cell damage caused by oxidized LDL. .

The black garlic extract is prepared by mixing black garlic and distilled water at a weight ratio of 1: 5 to 30, shaking the mixture at 100 DEG C and 100 to 140 rpm for 1 to 5 hours, filtering the extracted extract with a filter paper, , And the filtrate is obtained by concentration under reduced pressure using a vacuum rotation evaporator under the condition of 0.1 to 0.3 mPA for 5 to 7 hours.

More specifically, 1000 g of black garlic was added to 10 L of distilled water, and the mixture was shaken at 100 rpm and 120 rpm for 2 hours. The obtained extract was filtered through a filter paper (Whatman No. 2), and the filtrate was concentrated under reduced pressure at 0.2 mPA for 5 hours using a vacuum rotation evaporator to obtain 1 L of an extract.

It is a perennial herb that grows in the ponds and swamps of India, China, Korea, Japan, and Siberia. Roots, lotus leaves, lotus, and livestock are widely used for edible and medicinal purposes.

The rootstock contains 80 ~ 85% moisture, 12 ~ 15% carbohydrate, and contains trace amounts of protein and fat. It contains amino acids such as arginine, tyrosine, and trigonelline as well as about 2% asparagine. It contains a lot of phospholipid, lecithin, Lt; / RTI >

The extracts were prepared by mixing the dried rootstock powder and ethanol in a weight ratio of 1: 5 to 30, and then dipping them at room temperature for 20 to 30 hours. The extracted extract was filtered with a filter paper, 0.3 mPA for 5 to 7 hours using a vacuum rotation evaporator.

More specifically, dried root roots washed with water are dried and then ground to prepare a powder. 1,000 g of rootstock powder is immersed in 10 L of 50% ethanol and extracted at 23 캜 for 20 hours. The extracted extract was filtered with a filter paper (Whatman No. 2), and the filtrate was concentrated under reduced pressure for 5 hours at 0.2 mPA with a vacuum rotation evaporator to obtain 1 L of an extract.

4. Fourth Mixture Composition

The fourth mixed composition has a whitening function, and is mixed and blended at a ratio of 1: 1 weight of persimmon leaf extract and soil extract.

(+) - catechin, phloroglucinol, (+) - catechin-3-gallate, (+) - gallocatechin, and (+) - catechin as a mixture of various condensed tannins. It is known that falvan-3-ol compounds such as gallocatechin-3-gallate and condensed tannin belong to proanthocyanidin. Tannin compounds of persimmon leaves have an inhibitory effect on blood pressure rise, and condensed tannins have an effect of inhibiting free radicals of active oxygen. It also has antioxidant activity, anticancer and antimutagenic effects.

Persimmon leaves were mixed with persimmon leaves and ethanol at a weight ratio of 1: 5 ~ 30, and then subjected to reflux cooling extraction at 42 to 48 ° C for 2 to 6 hours. The extracted extract was centrifuged at 11,000 to 13,000 rpm for 20 to 40 minutes The supernatant is separated and the supernatant is filtered with a filter paper, and the filtrate is concentrated under reduced pressure using a vacuum rotation evaporator for 5 to 7 hours under the condition of 0.1 to 0.3 mPA. .

More specifically, 1,000 g of the persimmon leaf powder obtained by pulverizing and drying the persimmon leaves is added to 10 L of 80% ethanol, and the mixture is refluxed and cooled at 45 캜 for 6 hours. The obtained extract is centrifuged at 11,000 rpm for 40 minutes using a centrifuge to take the supernatant. The supernatant was filtered through a filter paper (Whatman No. 2), and the filtrate was concentrated under reduced pressure for 5 hours under vacuum of 0.2 mPA using a vacuum rotation evaporator to obtain 1 L of an extract.

Smilacis glabrae Rhizoma (SG) is a medicinal herb that refers to the rhizomes of Cynomolgus sinensis. The Cheongmaidae (Smilax china L.) is a shrub, and rhizomes extend sideways.

It has been used as a therapeutic drug for chronic skin diseases, gonorrhea, diuretic, and toxic skin diseases. Saponin such as parillin, smilacin, etc. Are reported as main components of soil bokyong. It contains ophiopogonin, a glycoside separated from roots and mucous materials, and plays an important role in protecting organ from immune enhancement and various bacterial infections.

The soil extract is prepared by mixing soil bacterium and ethanol at a weight ratio of 1: 5 to 30, followed by immersion at 50 to 80 ° C for 2 to 10 hours, extracting the extracted extract with a filter paper, Which is obtained by concentration under reduced pressure using a vacuum rotation evaporator under the condition of 0.1 to 0.3 mPA for 5 to 7 hours.

More specifically, 1,000 g of the shade and crushed red ginseng powder of Smilacis glabrae Rhizoma (SG) is immersed in 8 L of 95% ethanol and extracted at 65 ° C for 5 hours. The obtained extract was filtered through a filter paper (Whatman No. 2), and the filtrate was concentrated under reduced pressure at 0.2 mPA for 5 hours using a vacuum rotation evaporator to obtain 1 L of an extract.

5. Fifth mixed composition

The fifth mixed composition has an anti-aging function and is mixed at a weight ratio of 1: 1 of a tree leaf extract and an acacia flower extract.

Chestnut (Castanea crenata) is a deciduous broad-leaved arboreous tree belonging to oak.

Studies on chestnut (Castanea crenata) leaves are currently being carried out in various ways, but the range is not so wide.

The chestnut leaf extract was prepared by mixing chestnut leaf and ethanol at a weight ratio of 1:10 to 15, and then dipping the mixture at room temperature for 22 to 26 hours to separate the supernatant and the precipitate. The extracted extract was repeated three times to filter paper filter paper, and the filtrate is concentrated under reduced pressure at 58 to 63 ° C for 5 to 7 hours using a vacuum rotation evaporator.

Specific examples of the chestnut leaf extract include,

1,000 g of chestnut leaves are added to 10 L of 70% ethanol and then immersed at room temperature for 24 hours. The supernatant is separated from the supernatant and the supernatant is obtained, and this process is repeated twice. The supernatant is mixed and filtered with a filter paper.

The filtered extract was concentrated under reduced pressure at 62 DEG C and 0.2 mPA for 5 hours using a vacuum rotation evaporator to obtain 1 L of an extract.

Acacia (Robinia pseudoacacia) is a deciduous tree belonging to rosewood Congo.

The moisture content of the acacia petals was 86.60% and when dried, crude protein was 24.55%, crude ash was 8.51%, total sugar was 30.97%, ascorbic acid About 16%, and tannic acid is about 15%, which is known to have a relatively high content of sugar, protein, and ascorbic acid.

The acacia flower extract was prepared by mixing and drying powder of Acacia (Robiniapseudoacacia) after pulverization and purified water at a weight ratio of 1:35 to 45, followed by extraction at 85 to 99 ° C for 5 to 7 hours. The extracted extract was filtered through a filter paper After filtration, the filtrate was centrifuged at 2,000 ~ 4,000 rpm for 10 ~ 30 minutes in a centrifugal separator. The supernatant was filtered and the filtrate was vacuum-concentrated for 5 to 7 hours under the condition of 0.1 to 0.3 mPA evaporator) to obtain a solution.

More specifically, 1,000 g of dried acacia flowers are finely cut and mixed with 10 L of 50% ethanol, and the mixture is repeatedly extracted three times at 25 캜 for four hours. The obtained extract is filtered with a filter paper. The filtered extract was concentrated under reduced pressure at 0.2 mPA for 5 hours using a vacuum rotation evaporator to obtain 1 L of an extract.

≪ Second composition >

The second composition comprises 1.5 to 45.5 wt% Opuntia humifusa stem extract; Gossam extract 1.5 ~ 45.5 wt%; Kelp extract 1.5 to 45.5 wt%; Top extract 1.5 ~ 45.5 wt%; And 1.5 ~ 45.5 wt% of Daechu Seed Extract; it has a function to block the penetration of fine dust and to inhibit skin inflammation.

Examples of formulation of the second composition are shown in Table 2 below.

Formulation Example 1 Formulation Example 2 Formulation Example 3 Formulation Example 4 Formulation Example 5 Opuntia humifusa Stem Extract 20.0 wt% 10.0 wt% 30.0 wt% 15.0 wt% 20.0 wt% Gosam extract 25.0 wt% 20.0 wt% 10.0 wt% 30.0 wt% 20.0 wt% Seaweed extract 15.0 wt% 25.0 wt% 20.0 wt% 10.0 wt% 20.0 wt% Top extract 30.0 wt% 15.0 wt% 25.0 wt% 20.0 wt% 20.0 wt% Jujube Seed Extract 10.0 wt% 30.0 wt% 15.0 wt% 25.0 wt% 20.0 wt% Sum 100.0 wt% 100.0 wt% 100.0 wt% 100.0 wt% 100.0 wt%

Opuntiahumifusa is a cactus similar to Opuntia ficus-indica, widely known as Paekcheoncho. It is cultivated nationwide mainly in Chungcheongnam-do. It is used as raw material for food, medicine or cosmetics. The content of flavonoids in palm cactus is 5%, which is higher than that of yulmu (0.19%), altitude (0.21%), and root (2.21%). Dietary fiber content is 48.5%, fruits are 0.19 ~ 2.91%, vegetables are 0.99 ~ 7.42% It is higher than 1.19 ~ 10.35%, and vitamin C is 35mg / 100g, higher than aloe, inorganic, saponin, amino acid and complex polysaccharide are higher than other crops. Especially, It contains 4782.9mg of calcium, which is a necessary ingredient.

The Opuntiahumifusa stem extract was prepared by mixing Opuntiahumifusa stems at room temperature for 20 to 30 hours, mixing the crushed and pulverized 80% ethanol at a ratio of 1: 2 ~ 5, The filtrate was centrifuged at 2,000 ~ 4,000 rpm for 10 ~ 30 minutes in a centrifugal separator, and the supernatant was filtered. The filtrate was subjected to filtration using 0.1-0.3 mPa Under reduced pressure for 5 to 7 hours using a vacuum rotation evaporator.

As a specific example of the above-mentioned Opuntiahumifusa stem extract,

The Opuntiahumifusa stems were dried at room temperature for 25 hours. The pulverized material and 80% ethanol were mixed at a weight ratio of 1: 3 and then leached at room temperature for 25 hours. The extract was extracted with filter paper After filtration, the filtrate is centrifuged at 3,000 rpm for 15 minutes in a centrifuge, and the supernatant is filtered. The filtrate is concentrated under reduced pressure using a vacuum rotation evaporator for 6 hours under the condition of 0.2 mPA.

Gosam (Sophora falvescens Aiton) belongs to the bean family and is also called the nursery, the staff of the thief. It has been used in diabetes, dysentery, fever, eczema, gastrointestinal bleeding and antimicrobial action in the oriental medicine. It is clinically known to be externally used for eczema, skin pyoderma and skin diseases such as female genital warts and also for bacterial dysentery and enteritis .

The components of gosam known to date include alkaloids, flavonoids, saponins, etc., and contain 1 ~ 2% of alkaloids and 0.5% of flavonoids as main ingredients.

The extract is obtained by mixing gossam gum and ethanol at a weight ratio of 1:10 to 12 and then extracting at 68 to 72 ° C for 22 to 26 hours. The extract is filtered with a filter paper, The filtrate is obtained by concentration under reduced pressure using a vacuum rotation evaporator for 5 to 7 hours under the condition of 0.1 to 0.3 mPA.

Specific examples of the gosam extract include,

1,000 g of the raw body of fresh ginseng is added with 10 L of 90% ethanol, extracted at 70 ° C for 24 hours, and filtered through a reduced pressure filtration apparatus. The filtered extract was concentrated under reduced pressure at 62 DEG C and 0.2 mPA for 5 hours using a vacuum rotation evaporator to obtain 1 L of an extract.

Kelp is slightly different depending on the species, but it usually contains about 16% moisture, 7% protein, 1.5% fat, 49% carbohydrate and 26.5% mineral. 5 to 10% of the carbohydrates are fibrinous, and the remainder are polysaccharides such as laminarin and fucoidan mainly based on alginic acid (acid). The powder, mannitol, which is whitened on the surface of dried kelp, is known as the sweetness component of kelp.

The kelp extract is prepared by drying the kelp at 58 to 62 ° C for 10 to 14 hours in a hot air drier, mixing the pulverized dried powder and distilled water at a weight ratio of 1:15 to 20, mixing at 80 to 90 ° C for 1 to 3 hours The extract is heated and extracted to obtain an extract, and the extract is concentrated under reduced pressure using a vacuum rotation evaporator for 5 to 7 hours under the condition of 0.1 to 0.3 mPA.

More specifically, 1,000 g of finely ground dried kelp was added to 2 L of distilled water, followed by hot extraction at 82 DEG C for 25 minutes. The obtained extract was filtered through a filter paper (Whatman No. 2), and the filtrate was concentrated under reduced pressure at 0.2 mPA for 5 hours using a vacuum rotation evaporator to obtain 1 L of an extract.

Seaweed (Hizikia fusiforme) is a seagull with phytoplasma (Phaeophyta). The plant stands upright in the roots of the fibrous roots. The stem is circular, and the seaweed is split once into 羽 shaped.

The top extract is dried at 58 to 62 ° C for 10 to 14 hours in a hot air drier, mixed with dried powder and distilled water at a weight ratio of 1:15 to 20, and then dried at 80 to 90 ° C for 1 to 3 hours The extract was filtered with a filter paper, and the filtrate was concentrated under reduced pressure using a vacuum rotation evaporator for 5 to 7 hours under the condition of 0.1 to 0.3 mPA to obtain an extract use.

More specifically, after drying for 12 hours at 60 ° C in a hot air drier, the pulverized dried top powder and distilled water are mixed at a weight ratio of 1:15, and the mixture is heated at 80 ° C for 2 hours to obtain an extract. The extract was filtered through a filter paper (Whatman No. 2), and the filtrate was concentrated under reduced pressure at 0.2 mPA for 5 hours using a vacuum rotation evaporator to obtain 1 L of an extract.

Jujube (Zizyphus jujuba Miller, jujube) is a fruit of deciduous, leafy roots of the genus Zizyphus. Jujube has been known to be effective for the treatment of calming stabilizers, alleviation of digestion, stomach cure, tonic action, antiallergic, cholestasis, tuberculosis, hemorrhagic disease, nervous breakdown, colon cancer and bile.

Jujube contains a lot of carbohydrate and ascorbic acid. It contains various sterols and 30 kinds of alkaloids, sapogenin, vitamin C, P, K and rutin as medicinal ingredients.

The jujube seed extract was prepared by mixing the jujube seeds pulverized with a pulverizer and 80% methanol in a weight ratio of 1: 8 to 12, and then leaching at room temperature for 20 to 30 hours. The extracted extract was filtered with filter paper ), And the filtrate was centrifuged at 2,000 ~ 4,000 rpm for 10 ~ 30 minutes in a centrifuge. The supernatant was filtered and the filtrate was vacuum-evaporated for 5 to 7 hours under the condition of 0.1 to 0.3 mPA ) ≪ / RTI >

More specifically,

The extracts were filtered with a filter paper, and the filtrate was centrifuged at 3,000 rpm for 20 minutes at a centrifugal separator. , And the supernatant was filtered. The filtrate was concentrated under reduced pressure using a vacuum rotation evaporator at 0.2 mPA for 5 hours.

The first composition constituting the cosmetic composition for preventing penetration of fine dusts and preventing inflammation according to the present invention has moisturizing, antioxidant, skin protection and wrinkle improvement, whitening and antiaging function, and is characterized in that a mixture of sea cucumber extract and spirulina extract 0.5 to 60.0 wt% of the first mixed composition, 0.5 to 60.0 wt% of the second mixed composition prepared by mixing the flaxseed extract and the seed oil extract in the same weight ratio, the third mixed composition containing the black garlic extract and the pomegranate extract in a weight ratio of 0.5, A fourth mixture composition comprising 60.0 wt% of a persimmon leaf extract and a tobacco root extract in a weight ratio of 0.5 to 60.0 wt%, a chestnut leaf extract and an acacia flower extract in a weight ratio of 0.5 to 60.0 wt% .

Specifically,

20.0 wt% of the first mixed composition, 20.0 wt% of the second mixed composition, 20.0 wt% of the third mixed composition, 20.0 wt% of the fourth mixed composition, and 20.0 wt% of the fifth mixed composition.

The second composition constituting the cosmetic composition for preventing penetration of fine dusts according to the present invention comprises 1.5 to 45.5 wt% of Opuntia humifusa stem extract, which prevents skin infiltration of fine dusts and has skin inflammation inhibiting function, , 1.5 ~ 45.5 wt% of ginseng extract, 1.5 ~ 45.5 wt% of sea tangle extract, 1.5 ~ 45.5 wt% of top extract and 1.5 ~ 45.5 wt% of jujube extract.

Specifically,

It is composed of 20.0 wt% of Opuntia humifusa stem extract, 20.0 wt% of ginseng extract, 20.0 wt% of seaweed extract, 20.0 wt% of top extract, and 20.0 wt% of jujube seed extract.

The cosmetic composition according to the present invention for preventing penetration of fine dust and preventing inflammation is a composition comprising a first composition having a moisturizing function, a skin protection and a wrinkle improving function, a whitening function, a second composition having a skin penetration blocking function for fine dust, And is composed of a mixture of the first composition of the first embodiment and the second composition of the second embodiment.

More specifically, it is composed of a mixture of 60.0 wt% of the first composition and 40.0 wt% of the second composition (B).

In order to further enhance the fine dust adsorption function, the cosmetic composition for preventing penetration of fine dusts according to the present invention is prepared by further adding 0.1 to 10.0 wt% of Anemarrhena asphodeloides root extract to the second composition.

The extracts of Opuntia humifusa stem extracts were 1.5 ~ 45.5 wt%, the extracts were 1.5 ~ 45.5 wt%, the seaweed extracts were 1.5 ~ 45.5 wt%, the extracts were 1.5 ~ 45.5 wt%, the jujube extract was 1.5 ~ 45.5 wt%, the Anemarrhena asphodeloides root extract of 0.1 to 10.0 wt%.

More specifically,

A mixture of 20.0 wt% of stem extract of Opuntia humifusa, 18.0 wt% of ginseng extract, 18.0 wt% of kelp extract, 18.0 wt% of top extract, 18.0 wt% of jujube seed extract and 8.0 wt% of root extract of Anemarrhena asphodeloides .

The root extract of Anemarrhena asphodeloides is prepared by mixing the powder obtained by pulverizing Anemarrhena asphodeloides roots with distilled water at a weight ratio of 1: 5 to 10, heating and extracting at 100 ° C for 1 to 3 hours, filtered through filter paper, and the filtrate is concentrated under reduced pressure at 75 to 85 ° C for 5 to 7 hours using a vacuum rotation evaporator.

More specifically,

Anemarrhena asphodeloides powder was mixed with distilled water at a weight ratio of 1:10, and the mixture was heated at 100 ° C. for 2 hours. The extracted extract was filtered with a filter paper, and the filtrate was concentrated at 80 ° C. Which is obtained by concentration under reduced pressure using a vacuum rotation evaporator for 6 hours.

The Anemarrhena asphodeloides is a perennial herb roots belonging to the lily family. In traditional oriental medicine, it has been known for its efficacy as antipyretic, hypoglycemic, antiinflammatory, hypotensive, antibacterial and has been used for treatment of pulmonary disease, diabetes, constipation, and symptoms of poor urination.

The components contained in the gum are Steroidal saponins, Xanthone, C-glyco-sides, Polysaccharides and Norlignans. Primarily, steroidal saponins are the major component of the buds, and rootstocks of buds contain more than about 6% of total steroidal saponins.

In order to further enhance the fine dust adsorption function, the cosmetic composition for prevention of infiltration of fine dusts according to the present invention is prepared by further adding 0.1 to 10.0 wt% of Eisenia bicyclis extract to the second composition.

The extracts of Opuntia humifusa were 1.5 ~ 45.5 wt%, the extracts were 1.5 ~ 45.5 wt%, the seaweed extracts were 1.5 ~ 45.5 wt%, the extracts were 1.5 ~ 45.5 wt%, the jujube extract was 1.5 ~ 45.5 wt% bicyclis) extract of 0.1 to 10.0 wt%.

More specifically,

The mixture of Opuntia humifusa stem extract 20.0 wt%, ginseng extract 18.0 wt%, seaweed extract 18.0 wt%, top extract 18.0 wt%, jujube extract 18.0 wt% and Eisenia bicyclis extract 8.0 wt% do.

The Eisenia bicyclis extract was prepared by drying Eisenia bicyclis at 58 to 62 ° C for 10 to 14 hours in a hot air drier, mixing the pulverized dried powder and distilled water at a weight ratio of 1:15 to 20, The extract was filtered with a filter paper, and the filtrate was concentrated under reduced pressure using a vacuum rotation evaporator for 5 to 7 hours under the condition of 0.1 to 0.3 mPa The obtained product is used.

More specifically,

The dried powder of Eisenia bicyclis was dried at 60 ° C for 12 hours in a hot air drier, and the dried powder and distilled water were mixed at a weight ratio of 1:15, and the mixture was heated at 85 ° C for 2 hours. filter paper, and the filtrate obtained by concentration under reduced pressure with a vacuum rotation evaporator under the condition of 0.2 mPA for 6 hours is used.

The rhubarb (EiseniaBicyclis) is a perennial plant belonging to the seaweed (LAminariales) and Alariaceae. Rhubarb compared to other algae and fucoidan, but it contains laminaran, which is a phospholipid, and it has been reported that serum lipid lowering effect of rhubarb laminaran has been reported and antidiabetic and antioxidant functions as well as antidiabetic function of the dietary components have been reported. Also, rhubarb is a seaweed and has a high heavy metal adsorption capacity.

In order to further enhance the fine dust adsorption function, the cosmetic composition for prevention of infiltration of fine dusts according to the present invention is prepared by further adding 0.1 to 10.0 wt% of a buttercage extract to the second composition.

The extracts of Opuntia humifusa were 1.5 ~ 45.5 wt%, the extracts were 1.5 ~ 45.5 wt%, the seaweed extract was 1.5 ~ 45.5 wt%, the extracts were 1.5 ~ 45.5 wt%, the jujube extract was 1.5 ~ 45.5 wt% To 10.0 wt%.

More specifically,

The composition of Opuntia humifusa is composed of 20.0 wt% of stem extract, 18.0 wt% of ginseng extract, 18.0 wt% of kelp extract, 18.0 wt% of top extract, 18.0 wt% of jujube seed extract, and 8.0 wt% of farinacea extract.

The buttercage extract is prepared by mixing buttercup and ethanol at a weight ratio of 1: 5 to 30, and then extracting the mixture at 65 to 75 ° C for 20 to 30 hours. The extracted extract is filtered with a filter paper, Deg.] C, and 0.1 to 0.3 mPa for 5 to 7 hours using a vacuum rotation evaporator.

More specifically,

The mixture was mixed at a weight ratio of 1:20, and then extracted at 70 ° C for 25 hours. The extracted extract was filtered with a filter paper, and the filtrate was concentrated under reduced pressure at 0.2 MPa at 60 ° C for 6 hours, (vacuum rotation evaporator).

Mineral (Oenanthejavanica DC) is a dicotyledonous plant belonging to the family Mineral and Mt. It contains 94% of moisture, 1 ~ 2% of residual protein and lipid, and 2 ~ 3% of residual minerals and vitamins. It is a representative fiber type of alkali food. It contains Ca, P, Fe, B and C are abundant. As a result of the analysis of general components of parsley, it is known that leaves have high crude protein and crude fat content and roots have the highest crude fiber content.

The unique flavor and taste of buttercups are excellent for detoxifying the taste buds and releasing various toxins. It is a good food for hypertensive patients because it has the effect of lowering blood pressure when eating buttercups as juice. It is good for liver disease and is widely used for jaundice, acute and chronic hepatitis, and liver cirrhosis.

In order to further enhance the anti-inflammatory function, the cosmetic composition for prevention of penetration of fine dusts according to the present invention is prepared by further adding 0.1-10.0 wt% of a mugwort mugwort extract to the second composition.

Extract of Opuntia humifusa Stem Extract 1.5 ~ 45.5 wt% Extract of Extract of Ginseng Extract 1.5 ~ 45.5 wt% Extract of Seaweed Extract 1.5 ~ 45.5 wt% Extract of Extract Extract 1.5 ~ 45.5 wt% To 10.0 wt%.

More specifically,

It is composed of 20.0 wt% of Opuntia humifusa stem extract, 18.0 wt.% Of ginseng extract, 18.0 wt.% Of kelp extract, 18.0 wt.% Of top extract, 18.0 wt.% Of jujube seed extract and 8.0 wt.

The dried mugwort mugwort extract is prepared by mixing dried mugwort shrimp and distilled water at a weight ratio of 1:15 to 25, heating the mixture at 80 to 90 ° C for 1 to 3 hours, filtering the extracted extract with a filter paper, And the resultant is obtained by concentration under reduced pressure using a vacuum rotation evaporator for 5 to 7 hours under the condition of -0.3 mPA.

More specifically,

The extracts were filtered with a filter paper and the filtrate was concentrated under reduced pressure (0.2 mPa) for 6 hours in a vacuum concentrator vacuum rotation evaporator).

Chrysanthemum coronarium L was propagated from Europe, which is the origin of the country, to East Asia and used as a vegetable. Vitamin content is in a large number of pieces to 458 RE vitamin A, vitamin B ₁ is 0.11 mg, Vitamin B ₂ is 0.23 mg, the 15 mg of vitamin C, vitamin E 1.60 mg per 100 g. It also contains carotene, which is a lot of carrots. It is also rich in vitamin C, contains a large amount of lysine of essential amino acids, and contains calcium as a mineral.

In the outbreak of Saguscum, there are many flavonoid derivatives that exhibit antioxidative effects. These include caffeic acid, chlorogenic acid, 3,5-dicaffeoylquinic acid, 4-succinyl-3,5-dicaffeoylquinic acid, emodin, thiophene -isobutyl-6- (2-thiophenyl) -2,4-hexadienamide 1,8-dihydroxyanthroquinone, quercethin and its glycosides, rutin and isoquercetin.

In order to further prolong the preservation period, the cosmetic composition for prevention of penetration of fine dust and the composition for preventing inflammation according to the present invention is prepared by further adding 0.1 to 10.0 wt% of a third composition to the second composition.

The third composition comprises a mixture of 0.01 to 50.0 wt% of an acidic extract, 0.01 to 50.0 wt% of a moss extract, 0.01 to 50.0 wt% of an extract of Baekduong, and 0.01 to 50.0 wt% of a grapefruit seed extract. 25.0 wt.% Of mungbean extract, 25.0 wt.% Of moss extract, 25.0 wt.% Of Baekduong extract and 25.0 wt.% Of grapefruit seed extract.

0.01 to 3.0 wt% of the third composition thus prepared is further added to the second composition.

More specifically,

24.0 wt.% Of Opuntia humifusa stem extract, 24.0 wt.% Of ginseng extract, 24.0 wt.% Of sea tangle extract, 15.0 wt.% Of top extract, 10.0 wt.% Of jujube extract and 3.0 wt.% Of third composition.

The third composition has a function of extending the preservation period of cosmetic compositions and cosmetics as a natural preservative composed of an acidulant extract, a moss extract, a Baekjong extract, and a grapefruit seed extract.

Zanthoxylum schinfolium (Zanthoxylum schinfolium) is a species native to the altitude less than 1,000m altitude in Korea and its fruit is used as vegetable oil resources and various seasonings, and seed oil is also used as a lubricant for millet.

Sancho is the fruit of the sancho (Japanese apricot) tree. The fruit skin contains 2 ~ 4% of essential oil. Its main ingredients are dipentene (dl-limonene), citronellal, l-β-phellandrene, geraniol, citronellol Respectively. The fruit contains xanthoxine, a phlegmatic toxin, xanthoxine acid, a paralytic component, sterol campesterol, stigmasterol, and β-sitosterol.

The above-mentioned extract of the sancho extracts was mixed with the mixture of methanol and methanol at a weight ratio of 1:10 to 15, and then extracted with stirring at room temperature for 22 to 26 hours. The extracted extract was filtered with a filter paper, Under reduced pressure for 5 to 7 hours using a vacuum rotation evaporator.

As a specific example of the above-mentioned sancho extract, 1,000 g of anthocyanin and 10 L of methanol are mixed and extracted with stirring at room temperature for 24 hours. Filter paper No. 2, and concentrated under reduced pressure using a vacuum rotation evaporator at 62 DEG C and 0.2 mPA for 5 hours to obtain 1 L of an extract.

Moss has been reported to have excellent antimicrobial and antioxidant effects. When mosses are added to water, the acidity of the surrounding water is increased by the object of mosses in the body, thereby reducing the bacterial activity and preventing the deterioration of water.

There are lichen (moss, moss, Musci), umbrella (liverwort, Hepaticae), and horny lichen (hornwort, Anthocerotae). These lichens inhibit microbial propagation and are highly valuable as natural preservatives because of their antioxidant action.

The moss extract was washed with hot water at 55 to 65 ° C and then dried by hot air blow. The moss was mixed with 100% methanol at a weight ratio of 1:10 to 15, The extract was filtered with a filter paper and the filtrate was concentrated under reduced pressure with a vacuum rotation evaporator for 5 to 7 hours under the condition of 0.1 to 0.3 mPA Is used.

As a specific example of the moss extract, a moss mixture obtained by mixing moss, large feather moss and feather moss at the same weight ratio is dewatered by water washing and then dried by hot air drying at 62 ° C for 6 hours. 1,000 g of the pulverized dried sample and 10 L of 100% methanol are mixed in a reflux condenser equipped with a cooling tube and reflux cooled for extraction at 58 ° C. for 7 hours.

The extract was filtered with a pore size filter paper having a pore size of 5 μm and concentrated under reduced pressure at 62 ° C. and 0.2 mPA using a vacuum rotation evaporator for 5 hours to obtain 1 L of an extract.

It is a perennial plant belonging to the ranunculaceae family. It is distributed in all parts of Korea and also in northeast China. This herbal medicine has been commonly applied to toothache, fever, and water, and has been used for anti-inflammation, astringency, hemostasis, dysentery, detoxification, scrotum, antibacterial, sedation, and anticancer. It has been reported that this material has been widely used for anti-inflammation, analgesic, antipyretic, and detoxification purposes in Korea.

The Baekhwong extract is washed with water, dried in a freeze dryer, processed into a powder form, powdered Baekjong and distilled water are mixed at a weight ratio of 1:10 to 12, And the extracted extract is filtered with a filter paper and the filtrate is concentrated under reduced pressure using a vacuum rotation evaporator for 5 to 7 hours under the condition of 0.1 to 0.3 mPA.

As a specific example of the Baechuonggong extract, 1,000 g of Baekhwong in the form of powder is mixed with 10 L of distilled water to form a mixture, and the mixture is reflux-extracted at 80 ° C for 3 hours. Filter the extract with filter paper.

The filtered extract was concentrated under reduced pressure at 62 DEG C and 0.2 mPA for 5 hours using a vacuum rotation evaporator to obtain 1 L of an extract.

Grapefruit is juicy and has a bit of sour taste. Grapefruit seed extract is a natural antimicrobial material, and its main components are ascorbic acid, palmitic acid, glucoside, and naringin glycocortoferol, which inhibit mycotoxin biosynthesis and growth of fungal strains.

The grapefruit seed extract is prepared by mixing the grapefruit seed with ethanol at a weight ratio of 1: 5 to 10, and then refluxing cooled for extraction at a temperature of 60 to 62 ° C for 2 to 3 hours. The extracted extract is filtered with filter paper The filtrate is centrifuged at 10,000 × g for 20 to 40 minutes, and the supernatant obtained by concentrating the supernatant under reduced pressure for 5 to 7 hours under a condition of 0.1 to 0.3 mPA with a vacuum rotation evaporator is used.

As a specific example, grapefruit seed and ethanol were mixed at a weight ratio of 1: 8, and the mixture was refluxed and cooled for 2 hours at a temperature of 60 ° C. The extracted extract was filtered with a filter paper, For 30 minutes, and the supernatant obtained by concentrating the supernatant under reduced pressure for 5 hours under a condition of 0.2 mPA with a vacuum rotation evaporator.

1. Add 703.15 g DI Water, 2.0 g Carbopol, 50.0 g Glycerine + 0.05 g Xanthan gum, 2.0 g Allantoin, 5.0 g Betaine, , 1.0 g of arginine, 30.0 g of trehalose and 0.4 g of adenosine are mixed to prepare an aqueous phase.

5.0 g of Lipoid S75-3, 5.0 g of glycerol monostearate-105 (GMS 105), 5.0 g of Bees Wax, 10.0 g of Cetearyl Alcohol, 10.0 g of Shea butter g, 10.0 g of phenoxyethanol, 50.0 g of caprylic / capric triglyceride (MCT), 15.0 g of Tween 60, 15.0 g of Grape Seed Oil, Oil (25.0 g) and methyl parahydroxy benzoate (1.0 g) are melted by heating to make an oil phase.

3. The oil phase is mixed with the water phase to form a mixture.

4. When the temperature of the mixture falls below 38 ° C, 50 g of the cosmetic composition of Example 3, 5.0 g of cyclomethicone (DC345) and 0.4 g of perfume are added and stirred to complete the lotion.

In Comparative Example 1, the amount of purified water (D.I Water) was 753.15 g, and the lotion was completed without using the cosmetic composition according to the present invention.

The lotion is prepared in the same manner as in Example 9 except that 50.0 g of the cosmetic composition of Example 4 is used in the case of using the cosmetic composition.

The lotion is prepared in the same manner as in Example 9 except that 50.0 g of the cosmetic composition of Example 5 is used in the case of using the cosmetic composition.

The lotion is prepared in the same manner as in Example 9 except that 50.0 g of the cosmetic composition of Example 6 is used in the case of using the cosmetic composition.

The lotion is prepared in the same manner as in Example 9 except that 50.0 g of the cosmetic composition of Example 7 is used in the case of using the cosmetic composition.

The lotion is manufactured in the same manner as in Example 9 except that 50.0 g of the cosmetic composition of Example 8 is used in the case of using the cosmetic composition.

[ Test Example 1 ]

<Antioxidant measurement>

The antioxidative effects of the lotions according to Examples 9 to 14 are measured.

After diluting the lotion samples of Examples 9 to 14 and Comparative Example 1 with 50% dimethyl sulfoxide (DMSO), 80 쨉 l of each lotion sample was taken, and 10 mM DPPH (1,1-diphenyl-2 -picrylhydrazyl) solution was added thereto, vortexed for 15 seconds, 1 mL of distilled water was added, and the solution was allowed to stand at 40 DEG C for 35 minutes and the absorbance at 517 nm was measured.

As a result of measuring the antioxidative activity at 40 캜,

In Example 9 was 52.08%, Example 10 was 53.71%, Example 11 was 52.29%, Example 12 was 57.25%, Example 13 was 57.01%, Example 14 was 58.00%, and Comparative Example 1 was 11.29% .

The antioxidative effects of the cosmetic compositions containing the cosmetic composition using the natural extract according to the present invention were confirmed in the order of Example 14> Example 12> Example 13> Example 10> Example 9> It can be confirmed that Examples 9 to 14 have higher antioxidative activity than Comparative Example 1 although they show a slight difference in antioxidative activity depending on the formulation.

[ Test Example 2 ]

<Antimicrobial measurement>

The antibacterial effects of the lotions of Examples 9 to 14 are to be measured.

Antimicrobial tests were performed on the strains Staphylococcus aureus KCCM 11335, Escherichia coli, Pseudomonas aeruginosa KCCM 11802, Candida albicans KCCM 11282 and Aspagillusniger KCCM 60143.

E. coli, KCCM 40271), Candida albicans (C. albicans KCCM 11282), fungi (E. coli KCCM 11282), and yeast strains of Staphylococcus aureus (S. aureus, KCCM 11335), Pseudomonas aeroginosa (P. aeroginosa, KCCM 11802) and Escherichia coli Cultured Aspergillus niger (A. niger KCCM 60143) was cultured in a shaking incubator at 37 ° C and fungi at 26 ° C and 250 rpm.

The bacterial strains thus cultivated were inoculated into the cosmetics of Examples 9 to 14, and the viable cell counts were measured on days 1, 3 and 7, respectively. As a result of measurement, in each of Examples 9 to 14, Was not detected.

[ Test Example 3 ]

&Lt; Measurement of wrinkle improvement effect &

1. Elastase inhibition rate measurement

Elastase and N-succinyl- (Ala) 3-p-nitroanilide increased the activity. The degree of inhibition by 50% activity was measured by IC ₅₀ (㎍ / ml).

20 μl of the sample solution in which each of the cosmetic compositions of Examples 3 to 8 was diluted to 500 μg / ml and 10 μl of 2.5 U Elastase were added to 200 μl of the substrate 1.0 mM N-succinyl- (Ala) 3-p-nitroanilide After incubation at 25 ° C for 10 min, absorbance was measured at 410 nm with an ELISA reader. Elastase inhibition rate is expressed as the absorbance reduction rate of the addition of the sample solution and the non-addition solution as follows.

Inhibition rate (%) = {1- (absorbance of sample added / absorbance of non-additive)} x 100

The inhibition rate of Example 3 was 55.64%, the inhibition rate of Example 4 was 57.12%, the inhibition rate of Example 5 was 57.55%, the inhibition rate of Example 6 was 57.55% 56.74%, the inhibition rate of Example 7 was 57.71%, and the inhibition rate of Example 8 was 58.00%.

2. Measurement of collagenase inhibition rate

The degree of inhibition was measured by IC ₅₀ (㎍ / ㎖) at 50% reduction in activity by evaluating the activity of collagenase degrading collagen.

Collagen is dissolved in 20 mM Tris-HCl (pH 7.5) and 5 mM CaCl ₂ buffer solution to make 3 μg / ml of substrate solution. 200 μl of Buffer, 20 μl of the substrate solution, 20 μl of the sample solution diluted to 500 μg / ml of each of the cosmetic compositions of Examples 3 to 8 was added, and the mixture was reacted at 37 ° C. for 1 hour with addition of collagenase. 100 μl of 2% ninhydrin reagent was added and incubated for 20 minutes. Absorbance was measured at 570 nm using an ELISA reader.

As a result of measurement, the inhibition rate in Example 3 was 78.11%, the inhibition rate in Example 4 was 79.01%, the inhibition rate in Example 5 was 78.13%, the inhibition rate in Example 6 was 79.56%, the inhibition rate in Example 7 was 79.17% Was inhibited by 80.24%.

The results of the Elastase inhibition rate and Collagenase inhibition rate of the cosmetic compositions of Examples 3 to 8 confirmed that wrinkles were improved, and the effect of improving the wrinkles of the cosmetic compositions containing the cosmetic compositions could be predicted.

[ Test Example 4 ]

<Measurement of whitening effect>

The melanocyte present in the basal layer of the skin protects the skin by synthesizing melanin from tyrosine, which is stimulated by ultraviolet rays. However, excessive production of melanin can cause aesthetic and skin health problems, thus protecting the skin from ultraviolet rays or inhibiting the production of melanin.

In order to examine the whitening efficacy of cosmetics, twelve women aged 20 to 40 years without skin disease were selected as subjects to be tested for inhibition of tyrosinase activity and inhibition of melanin production.

1. Measurement of inhibition rate of tyrosinase activity

Tyrosinase is the only enzyme involved in the biosynthesis of melanin, the pigment component of the skin. The formula for the inhibition of tyrosinase activity is as follows.

Inhibition rate of tyrosinase activity (%)

= 100 - [(b-b ') / (a-a')] 100

a: Absorbance after reaction of blank sample

b: Absorbance after reaction of the sample solution

a ', b': absorbance measured by replacing with tyrosinase buffer

0.5 ml of 0.02 M sodium phosphate buffer (pH 6.5), 0.2 ml of 10 mM L-DOPA substrate and 0.1 ml of the cosmetic composition selected from Examples 3 to 8 were separately added to a 96-well plate, followed by addition of mushroom tyrosinase 110 Unit / mL) was added and reacted at 37 ℃ for 2 minutes. Absorbance was measured at 475 ㎚.

As a comparative example, albutin was used as a control group.

In order to confirm the inhibitory effect of tyrosinase, albutin (comparative example) used in cosmetics as a whitening agent in place of the cosmetic composition according to Examples 3 to 8 (comparative example) and in Examples 3 to 8 The whitening activity of the cosmetic composition according to the present invention was compared.

The whitening activity of 0.1% albutin in the comparative example was measured to be 68.53%.

The whitening activity of Example 3 was 65.04%; The whitening activity of Example 4 was 62.11%; The whitening activity of Example 5 was 63.64%; The whitening activity of Example 6 was 61.27%; The whitening activity of Example 7 was 62.75%; The whitening activity of Example 8 was 62.97%; showing a high activity almost similar to that of albutin used in medicinal products and cosmetics as a whitening agent.

Accordingly, it can be seen that the whitening effect of the cosmetic composition according to Examples 3 to 8 according to the present invention is large.

2. Melanin production inhibition

B16 melanoma cells were plated at 3 ㎖ in 6 well plates and cultured in phenol red-free DMEM solution containing 10% FBS for 12 hours.

Each of the cosmetic compositions according to Examples 3 to 8 was pre-treated at 36 ° C for 12 minutes, treated with 1 μM MSH, and cultured at 37 ° C for 48 hours.

After incubation, add 100 μL of 10 mM sodium phosphate buffer (pH 6.8) containing 1% (w / v) Triton X-100, shake for 5 minutes, and transfer to an eppendorf tube.

The cell pellet obtained by centrifugation at 3,000 rpm for 5 minutes was added with 100 μL of 1 N NaOH and 200 μL of distilled water. The melanin was completely dissolved by incubation at 60 ° C. for 1 hour. Then, 200 μL was transferred to a 96-well plate and absorbance was measured at 405 nm Respectively.

The absorbance at 490 nm wavelength was measured and compared using a Microplate Reader (Infinite 200, Tecan Group Ltd., Switzerland).

Inhibition (%) = [1- (A1 / A0)] 100

A0: Absorbance of non-additive (control)

A1: Absorbance of sample added sphere

As a result, the inhibitory activity of Example 3 was 64.11%; The inhibition of Example 4 was 65.54%; The inhibition of Example 5 was 65.76%; The inhibitory potency of Example 6 was 66.11%; The inhibition of Example 7 was 66.59%; It was confirmed that the inhibitory activity of Example 8 was 66.14%;

As a result of the tyrosinase activity inhibition result and the inhibition of melanin formation, it can be expected that the cosmetic composition according to the present invention will have a high whitening effect when applied to cosmetics.

[ Test Example 5 ]

<Anti-Aging Measurement>

The anti-aging effects of the cosmetic composition according to Examples 3 to 8 will be measured.

Skin aging caused by photoaging during skin aging is caused by irregular pigmentation, skin sagging, vasodilation, reduced elasticity, blistering and deep and thick wrinkles caused by exposure to ultraviolet rays, and the skin becomes very rough and dry. In particular, the amount of collagen in the skin is reduced and the elastic fibers are denatured, resulting in wrinkles.

Due to the action of ultraviolet rays, the skin over-produces harmful reactive oxygen species (ROS) including superoxide anion, peroxide, singlet oxygen and the like, Low molecular weight antioxidants such as enzymes and glutathione, vitamin E, vitamin C and ubiquinol are reduced. Oxidative stress induces lipid peroxidation, protein oxidation, activation of proteolytic enzymes that destroy epilepsy, destruction of elastic fibers such as collagen and elastin, damage of cellular components, and promotes photoaging.

The reactive oxygen species (ROS) is an intermediate product produced when oxygen molecules are incompletely reduced during the cell respiration process, and refers to oxygen having a free radical. ROS occurs naturally by the normal metabolism of oxygen and plays an important role in cell signaling and homeostasis. The concentration of ROS can dramatically increase due to environmental stresses such as exposure to ultraviolet radiation or high heat.

The increase of ROS causes oxidative stress, which causes skin diseases such as photoaging and pigmentation.

In order to investigate the effect of the cosmetic composition according to Examples 3 to 8 on the photoaging effect, experiments were carried out using HaCaT cell (ATCC, Manassas, VA, USA) which is a human keratinocyte cell line.

HaCaT cells were cultured in a medium supplemented with normal medium (Comparative Example 1) or 100 μg / ml of the cosmetic composition according to Examples 3 to 8 for 24 hours, irradiated with UVB 80 mJ / cm 2, and then measured with a Multi-Mode Microplate Reader Respectively.

Compared with Comparative Example 1 in which HaCaT cells were irradiated with UVB 80 mJ / cm 2,

When treated with the cosmetic composition according to Example 3, the ROS was 75.4%

When treated with the cosmetic composition according to Example 4, ROS was 73.4%

When treated with the cosmetic composition according to Example 5, the ROS was 76.7%

When treated with the cosmetic composition according to Example 6, the ROS was 74.1%

When treated with the cosmetic composition according to Example 7, the ROS was 78.5%

When treated with the cosmetic composition according to Example 8, the ROS decreased to 78.2%. From these results, it was confirmed that the cosmetic composition according to the present invention has an inhibitory effect on active oxygen, and the anti-aging effect of the cosmetic product can be predicted.

[ Test Example 6 ]

The effect of the lotion prepared according to Examples 9 to 14 on the prevention of penetration of fine dust into the skin and the effect on the inflammation-inhibiting function is measured.

<Effect of prevention of fine dust penetration>

In order to confirm the skin penetration blocking effect of the fine dust by the lotion prepared according to Examples 9 to 14, the fine dust adsorption rate of the lotion was measured.

The lotion and 100 ppm of fine dust were allowed to react for 15 minutes, and the adsorption rate was measured 10 times by atomic absorption spectrometry. As a result, the average adsorption rate (%) of Examples 9 to 14 was measured as 87 .

Thus, it can be confirmed that the skin penetration blocking effect of the fine dust of the lotion prepared according to Examples 9 to 14 is excellent.

&Lt; Effect of inhibiting inflammation &

In order to measure the inhibitory effect of the skin inflammation factors MMP-1 and PGE-2, which were produced according to Examples 9 to 14, on the expression of MMP-1 and PGE-2, The percent inhibition of MMP-1 expression and the percent inhibition of PGE-2 expression were measured for each lotion prepared according to Examples 9-14, by reacting with the lotion prepared according to Examples 9-14.

The mean MMP-1 expression inhibition rate (%) was 80% and the PGE-2 expression inhibition rate (%) was 79%. Thus, it can be confirmed that the lotion prepared according to Examples 9 to 14 has an anti-inflammatory effect.

The cosmetic composition comprising the natural extract according to the present invention as an active ingredient and having a function of inhibiting skin infiltration of skin and inhibiting skin inflammation, and a functional cosmetic composition using the same, can prevent skin infiltration, It has an anti-inflammatory function, has antibacterial and antioxidant functions added to natural extracts, and has a merit of being cleaner and safer to use due to blocking of bacterial infection and the like, which is highly likely to be used industrially.

Claims (7)

The mixture of sea cucumber and ethanol was mixed at a weight ratio of 1:10 to 12, and the mixture was extracted with shaking incubator at 120 to 160 rpm at 24 to 26 ° C for 65 to 75 hours. The extracted extract was centrifuged at 7,500 to 8,500 rpm for 15 to 25 Min. The supernatant obtained by centrifuging was filtered with a filter paper, and the filtrate was concentrated under reduced pressure for 5 to 7 hours under a condition of 0.1 to 0.3 mPa by a vacuum rotation evaporator.
Spirulina and distilled water were mixed at a weight ratio of 1: 5 to 30, and the mixture was shaken in an incubator at 20 to 30 ° C and 130 to 160 rpm for 50 to 80 hours. The extracted extract was centrifuged at 7,000 to 9,000 rpm for 10 The supernatant obtained by centrifugation for 30 minutes was filtered with a filter paper, and the filtrate was vacuum-evaporated using a vacuum rotation evaporator at 0.1 to 0.3 mPa for 5 to 7 hours. 0.5 to 60.0 wt% of the first mixed composition (a1) prepared by mixing the spirulina extract obtained by concentrating under reduced pressure with the above mixture at a same weight ratio;
Flaxseed powder and ethanol are mixed at a weight ratio of 1:10 to 15, and then the mixture is dipped at 50 to 80 ° C for 2 to 10 hours to extract. The extracted extract is filtered with a filter paper, For 5 to 7 hours under reduced pressure using a vacuum rotation evaporator,
After mixing the seeds and distilled water at a weight ratio of 1: 1, the mixture is heated and extracted at 100 ° C for 1 to 3 hours. The extracted extract is filtered with a filter paper, and the filtrate is stirred at 75 to 85 ° C for 5 to 7 hours 0.5 to 60.0 wt% of a second mixed composition (a2) prepared by blending the seed oil extracts obtained by concentration under reduced pressure with a vacuum rotation evaporator in the same weight ratio;
The mixture of black garlic and distilled water was mixed at a weight ratio of 1: 5 to 30, and the mixture was shaken for 1 to 5 hours at 100 DEG C and 100 to 140 rpm. The extracted extract was filtered with a filter paper, The extract of black garlic obtained by concentration under reduced pressure with a vacuum rotation evaporator for 5 to 7 hours under the condition of ~ 0.3 mPA,
After mixing the dried rootstock powder with ethanol at a weight ratio of 1: 5 to 30, the mixture is immersed at room temperature for 20 to 30 hours to extract the extract. The extracted extract is filtered with filter paper, and the filtrate is filtered under conditions of 0.1 to 0.3 mPa 0.5 to 60.0 wt% of a third mixed composition (a3) prepared by blending the extracts of L. roots obtained by concentration under reduced pressure with a vacuum rotation evaporator for 5 to 7 hours in the same weight ratio;
The mixture of persimmon leaves and ethanol was mixed at a weight ratio of 1: 5 ~ 30, and the mixture was reflux cooled for 2 ~ 6 hours at 42 ~ 48 ℃. The extract was centrifuged at 11,000 ~ 13,000 rpm for 20 ~ The supernatant was filtered with a filter paper, and the filtrate was concentrated under reduced pressure using a vacuum rotation evaporator for 5 to 7 hours under the condition of 0.1 to 0.3 mPA.
The mixture of soil and ethanol was mixed at a weight ratio of 1: 5 ~ 30, and the mixture was immersed at 50 ~ 80 ℃ for 2 ~ 10 hours. The extracted extract was filtered with a filter paper to obtain a filtrate of 0.1 ~ 0.3 mPA 0.5 to 60.0 wt% of a fourth mixed composition (a4) in which the soil extract obtained by concentration under reduced pressure with a vacuum rotation evaporator for 5 to 7 hours is mixed in the weight ratio;
Chestnut leaf and ethanol were mixed at a weight ratio of 1:10 to 15, and then the mixture was immersed at room temperature for 22 to 26 hours to separate the supernatant and the precipitate. The same procedure was repeated three times, and the extracted extract was filtered with a filter paper , Chestnut leaf extract obtained by concentrating the filtrate under reduced pressure at 58 to 63 ° C for 5 to 7 hours with a vacuum rotation evaporator,
After drying and finishing the Acacia (Robiniapseudoacacia) flower, the powder is mixed with the purified water at a weight ratio of 1:35 to 45, and then extracted at 85 to 99 ° C for 5 to 7 hours. The extracted extract is filtered with filter paper , And the filtrate was centrifuged at 2,000 ~ 4,000 rpm for 10 ~ 30 minutes in a centrifuge. The supernatant was filtered and the filtrate was concentrated under reduced pressure using a vacuum rotation evaporator for 5 to 7 hours under the condition of 0.1 to 0.3 mPA (A1 + a2 + a3 + a4 + a5) of 0.5 to 60.0 wt% of a fifth mixed composition (a5) prepared by mixing the obtained acacia flower extract in a weight ratio of 100.0 wt% ) 50.0 to 95.0 wt%
The Opuntiahumifusa stems were dried at room temperature for 20 to 30 hours. The pulverized material was mixed with 80% ethanol at a weight ratio of 1: 2 to 5, and then the mixture was extracted at room temperature for 20 to 30 hours for extraction. Was filtered through a filter paper, and the filtrate was centrifuged at 2,000-4,000 rpm for 10-30 minutes in a centrifugal separator. The supernatant was filtered and the filtrate was filtrated for 5 to 7 hours at 0.1 to 0.3 mPa 1.5 to 45.5 wt% of Opuntia humifusa stem extract (b1) obtained by concentration under reduced pressure with a vacuum rotation evaporator;
The extract was filtered through a filter paper, and the filtrate was filtered to remove the filtrate. The filtrate was filtered through a filter paper, 1.5 to 45.5 wt% of the extract (b2) obtained by concentration under reduced pressure with a vacuum rotation evaporator for 5 to 7 hours under the condition of 0.3 mPA;
The kelp was dried in a hot air dryer at 58 to 62 ° C for 10 to 14 hours, and the pulverized dried powder and distilled water were mixed at a weight ratio of 1:15 to 20, and the mixture was heated and extracted at 80 to 90 ° C for 1 to 3 hours. 1.5 to 45.5 wt% of an extract of sea tangle (b3) obtained by concentrating the extract with a vacuum rotation evaporator under the condition of 0.1 to 0.3 mPA for 5 to 7 hours under reduced pressure;
The dried powder was dried at 58 to 62 ° C in a hot air drier for 10 to 14 hours. The pulverized dried powder and distilled water were mixed at a weight ratio of 1:15 to 20, and the mixture was heated and extracted at 80 to 90 ° C for 1 to 3 hours. (B4) obtained by concentrating the filtrate under reduced pressure with a vacuum rotation evaporator under the condition of 0.1 to 0.3 mPA for 5 to 7 hours, and then extracting the extract with a filter paper. 1.5 to 45.5 wt%;
The jujube seeds were mixed with 80% methanol at a ratio of 1: 8 ~ 12 by weight and then extracted at room temperature for 20 ~ 30 hours. The extracted extract was filtered with a filter paper, The filtrate was centrifuged at 2,000 to 4,000 rpm for 10 to 30 minutes in a centrifuge, and the supernatant was filtered. The filtrate was concentrated under reduced pressure using a vacuum rotation evaporator for 5 to 7 hours at 0.1 to 0.3 mPa And 5.0 to 50.0 wt% of a second composition (B) of 100.0 wt% composed of a mixture (b1 + b2 + b3 + b4 + b5) of the obtained jujuba extract (b5) of 1.5 to 45.5 wt% As an active ingredient, to inhibit skin penetration of fine dusts and to inhibit skin inflammation.
The method according to claim 1,
The second composition (B) is prepared by mixing powders obtained by pulverizing Anemarrhena asphodeloides roots with distilled water at a weight ratio of 1: 5 to 10, heating the mixture at 100 ° C for 1 to 3 hours, extracting the extracted composition with filter paper and further comprising 0.1 to 10.0 wt% of root extract of Anemarrhena asphodeloides obtained by concentrating the filtrate under reduced pressure at 75 to 85 ° C for 5 to 7 hours with a vacuum rotation evaporator, Wherein the cosmetic composition has a function of blocking penetration of fine dusts and inhibiting skin inflammation.
The method according to claim 1,
The second composition (B) was prepared by drying Eisenia bicyclis at 58 to 62 ° C for 10 to 14 hours with a hot air drier, mixing the pulverized dried powder with distilled water at a ratio of 1:15 to 20, The extract was filtered with a filter paper, and the filtrate was concentrated under reduced pressure using a vacuum rotation evaporator for 5 to 7 hours under the condition of 0.1 to 0.3 mPA. And 0.1 to 10.0 wt% of an extract of Eisenia bicyclis as an active ingredient. The cosmetic composition of the present invention has a function of inhibiting skin penetration and preventing skin inflammation of fine dusts.
The method according to claim 1,
The second composition (B) is prepared by mixing buttercup and ethanol in a weight ratio of 1: 5 to 30, and then extracting the mixture at 65 to 75 ° C for 20 to 30 hours. The extracted extract is filtered with filter paper, And 0.1 to 10.0 wt% of a buttercage extract obtained by concentration under reduced pressure using a vacuum rotation evaporator at a temperature of 58 to 63 ° C and 0.1 to 0.3 mPA for 5 to 7 hours, Wherein the cosmetic composition has a function of blocking penetration of fine dust and preventing skin inflammation.
The method according to claim 1,
The second composition (B)
Dried chesagtut and distilled water are mixed at a weight ratio of 1:15 to 25, and the mixture is heated at 80 to 90 ° C for 1 to 3 hours. The extracted extract is filtered with a filter paper, And 0.1 to 10.0 wt% of the extract of Mugwort mugwort obtained by concentration under reduced pressure with a vacuum rotation evaporator for 5 to 7 hours under the condition that the microparticles contain the natural extract as an active ingredient. A cosmetic composition having a function of inhibiting skin inflammation.
The method according to claim 1,
The second composition (B)
The mixture was mixed with methanol at a weight ratio of 1:10 to 15, and then extracted with stirring at room temperature for 22 to 26 hours. The extracted extract was filtered with a filter paper, and the filtrate was filtered under a condition of 0.1 to 0.3 mPa 0.01 to 50.0 wt% of an acid extract obtained by concentration under reduced pressure using a vacuum rotation evaporator for 7 hours;
The moss was washed with hot air at 55 to 65 ° C and then pulverized. The pulverized moss and 100% methanol were mixed at a weight ratio of 1:10 to 15, and the mixture was cooled to a temperature of 58 to 62 ° C at a temperature of 58 to 62 ° C The extract was filtered with a filter paper, and the filtrate was concentrated under reduced pressure using a vacuum rotation evaporator for 5 to 7 hours under the condition of 0.1 to 0.3 mPA. 0.01 to 50.0 wt% of extract;
Dried in a freeze drier, processed into powder form, powdered Baekjong and distilled water were mixed at a weight ratio of 1:10 to 12 times, and the mixture was refluxed at 75 to 85 ° C for 2 to 4 hours 0.01 to 50.0 wt% of Baechutong extract obtained by vacuum filtration of the filtrate with a vacuum rotation evaporator under the condition of 0.1 to 0.3 mPA for 5 to 7 hours, and extracting the extracted extract with filter paper.
The mixture of grapefruit seed and ethanol is mixed at a weight ratio of 1: 5 to 10, and the mixture is refluxed and cooled at a temperature of 60 to 62 ° C for 2 to 3 hours. The extracted extract is filtered with a filter paper, × g for 20 to 40 minutes, and the obtained supernatant was concentrated with a vacuum rotation evaporator under a reduced pressure of 0.1 to 0.3 mPA for 5 to 7 hours to obtain a mixture of a grapefruit seed extract of 0.01 to 50.0 wt% Wherein the cosmetic composition further comprises 0.01 to 3.0 wt% of a third composition of 100.0 wt%, which is composed of 100.0 wt% of the first composition.
The cosmetic composition according to any one of claims 1 to 6, which is prepared by adding 0.1 to 50.0 wt% of the cosmetic composition so as to be included in the total weight of the cosmetic, Cosmetics with anti-inflammatory properties.