KR20090055309A - A cosmetic composition for skin whitening and treating wrinkles - Google Patents

Abstract

A cosmetic composition for skin whitening and wrinkle improvement is provided to activate a skin cell, improve the wrinkle, collagen biosynthesis, and whitening. A cosmetic composition for external use comprises 2-7 weight part of red ginseng extract, 2-7 weight part of skullcap extract, 2-7 weight part of tomato extract, 2-7 weight part of Cnidii Rhizoma extracts, 2-7 weight part of jasmine extract, 2-7 weight part of cucumber extract, 2-7 weight part of pine needle extract, 0.1-1 weight part of peppermint extract, 2-7 weight part of lavender, 2-7 weight part of Jujube extract, and 2-7 weight part of Sophora flavescens Aiton. The extracts are obtained by isolating with water, low alcohol of 1-4 carbon atoms, or their mixture solvent. The composition is used in a form of solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray.

Description

A cosmetic composition for skin whitening and treating wrinkles}

The present invention relates to an external skin cosmetic composition for skin whitening and wrinkle improvement, more specifically, bamboo vinegar liquid, red ginseng extract, golden extract, tomato extract, celery extract, jasmine extract, cucumber extract, pine needle extract, willow extract, mint It contains extracts, magnolia flower extracts, lavender extracts, jujube extracts, red ginseng extracts, and licorice extracts as active ingredients to activate skin cells, improve wrinkles, promote collagen biosynthesis and whitening, It relates to a cosmetic composition that improves pigmentation and is safe for skin.

Skin is the body's primary protective film that protects organs in the body from changes in temperature and humidity, irritation from the external environment such as UV and pollutants, and plays an important role in maintaining homeostasis such as body temperature control. However, cell damage stimuli, such as excessive physical and chemical stimuli and stress, malnutrition, ultraviolet radiation, environmental pollutants, and sudden changes in temperature, applied from outside the cell, induce a variety of biochemical changes in the cell, causing a cellular damage response. Among these, skin aging is recognized to be due in part to changes in the physiological function of the skin resulting from the oxidative damage response caused by these external stresses. In other words, if cell membranes of skin cells are continuously damaged by free radicals or other radicals, the cross-linkage of proteins or lipoproteins is increased or degraded, and the amount of lipid peroxide is increased. When the cells and cell membranes are damaged, the permeability of the cell membranes is changed, which disrupts the ion balance in the cells, and this change prevents the release of intracellular metabolites and deteriorates the cells' ability to detoxify these metabolites. If such cellular level changes persist, the tissue structure loses regularity. Local scars appear on the dermis, and the thickness of the dermis decreases due to the reduction of collagen fibers or elastic fibers. Crosslinking between collagen fibers also increases and the fine structure of the elastic fibers is also damaged, resulting in a decrease in the elasticity of the skin as a whole. Factors affecting this include hydrogen peroxide (H ₂ O ₂ ), superoxide anion, and hydroxy radicals. These harmful reactive oxygen species reduce the elasticity of the dermis and blacken the skin. Will also be involved. In other words, oxidative damage of skin cells by external stimuli ultimately promotes aging and blackening of the skin.

As a result, the appearance of the skin promotes skin problems such as blemishes, freckles, blemishes, loss of elasticity, keratinization, and wrinkle formation and aging. To prevent such a phenomenon and maintain a healthier and more elastic skin, various animals, plants Efforts have been made to effectively inhibit skin aging by maintaining skin's intrinsic function and activating skin cells by using cosmetics containing bioactive substances obtained by microorganisms and organic synthesis.

Such materials include ascorbic acid, kojic acid, arbutin, hydroquinone, glutathione, and derivatives thereof. Of these, hydroquinone exhibits a predetermined whitening effect but has severe skin irritation, so that the amount of the compound should be limited to a very small amount. In addition, ascorbic acid is easily oxidized, and the cosmetics containing the same cause problems such as discoloration and discoloration. Since kojic acid is unstable in a solution, the manufacturing process of the cosmetic is complicated. In addition, thiol-based compounds such as glutathione and cysteine not only have a characteristic unpleasant odor, but also have problems with transdermal absorption, and glycosides and derivatives thereof have a high polarity and thus have problems in being used as a component of cosmetics. On the other hand, placenta extracts, which are frequently used these days, are not irritating to the skin but have insufficient whitening effects.

Therefore, studies investigating the whitening activity centered on natural extracts have been activated, Sangbaekpi (Japanese Patent Publication No. 55-44375, Small 64-26507, Small 64-83009, Pyeong 1-25687, Pyeong 5-139950 and Korea Patent Publication 92-002109, 97-021273), licorice (Japanese Patent Publication No. 60-214721, Small 60-214728, Small 63-23809, Small 64-63506, Pyeong 1-149706 and Korean Patent Publication 92-002109, 97-025601) , Peony (Japanese Patent Laid-Open No. 61-246109 and Korean Patent Laid-Open No. 92-002111), Cinnamon (Japanese Laid-Open Patent No. 63-30403, Hep. 5-139954), Gosam (Japanese Laid-Open Patent No. 64-26507 and Korean Laid-Open Patent 92- 002110), brown root (Japanese Patent Laid-Open No. 60-214727, small 64-16709), Angelica (Japanese Laid-open Patent No. 56-92211, Hei 4-26610), Bark skin (Japanese Laid-open Patent No. 60-214721, Small 61-50915, 61-246109), Banha (Japanese Patent Laid-Open No. 2-207028, Korean Patent Laid-Open No. 96-033442), Aloe (Japanese Patent Laid-Open No. 52-44375, Hei 2-207030, Hei 5-139950) and many plant extracts; Herbal extracts such as tyrosinase It has been found to act on to inhibit melanin production. However, they also have a lot of problems in the use of more than the effective concentration in cosmetics or pharmaceuticals in terms of safety, discoloration, etc., the effect is also insignificant.

Therefore, there is a need for a functional cosmetic having excellent skin anti-aging and whitening effect without causing skin side effects.

Therefore, it is an object of the present invention to provide an external skin cosmetic composition that can be safely used without side effects on the skin, as well as excellent wrinkle improvement effect, collagen biosynthesis effect, whitening effect and the like.

In order to achieve the above object, the present invention is bamboo shoot liquid, red ginseng extract, golden extract, tomato extract, celery extract, jasmine extract, cucumber extract, pine needle extract, willow extract, peppermint extract, magnolia flower extract, lavender extract, jujube It provides a cosmetic composition for external skin comprising the extract, high ginseng extract and licorice extract as an active ingredient.

2 to 7 parts by weight of bamboo vinegar, 2 to 7 parts by weight of red ginseng extract, 2 to 7 parts by weight of golden extract, 2 to 7 parts by weight of tomato extract, 2 to 7 parts by weight of uterine extract, 2 to 7 parts by weight Parts Jasmine Extract, 2-7 parts Cucumber Extract, 2-7 parts Pine Needle Extract, 2-7 parts Willow Extract, 0.1-1 part Peppermint Extract, 2-7 parts Magnolia Extract, 2-7 parts It is characterized by comprising a part by weight of lavender extract, 2 to 7 parts by weight of jujube extract, 2 to 7 parts by weight of ginseng extract and 1 to 3 parts by weight of licorice extract.

The cosmetic composition for external skin may be a fat or oil component, a humectant, an emollient, a surfactant, an organic and inorganic pigment, an organic powder, an ultraviolet absorber, a preservative, a fungicide, an antioxidant, a plant extract, a pH adjuster, It may further include one or more additives selected from the group consisting of alcohols, pigments, flavorings, blood circulation accelerators, cooling agents, limiting agents, purified water and carriers.

The cosmetic composition for external skin of the present invention is bamboo vinegar extract, red ginseng extract, golden extract, tomato extract, celery extract, jasmine extract, cucumber extract, pine needle extract, willow extract, peppermint extract, magnolia flower extract, lavender extract, jujube extract, high ginseng extract And licorice extract as an active ingredient to activate skin cells, improve wrinkles, promote collagen biosynthesis, whitening effect, improve pigmentation of blemishes, freckles, etc., and are safe for skin.

EMBODIMENT OF THE INVENTION Hereinafter, the structure of this invention is demonstrated concretely.

The present inventors simultaneously added extracts of gold, tomato, cucumber, jujube, licorice and the like, and extracts of bamboo vinegar, red ginseng, and celery to the cosmetic composition, which showed an excellent inhibitory effect on tyrosinase activity and proved to be excellent in inhibiting melanin synthesis. Synthetic tyrosinase activity inhibitory effect when shown to significantly increase the whitening effect of the cosmetic composition including these as well as to increase the proliferation effect and collagen biosynthesis effect of skin cells, thereby confirming the wrinkle improvement effect Was completed.

Therefore, the present invention is bamboo vinegar extract, red ginseng extract, golden extract, tomato extract, celery extract, jasmine extract, cucumber extract, pine needle extract, willow extract, peppermint extract, magnolia extract, lavender extract, jujube extract, high ginseng extract and licorice extract It provides a cosmetic composition for external skin containing as an active ingredient.

The cosmetic composition for external skin of the present invention is 2 to 7 parts by weight of bamboo vinegar, 2 to 7 parts by weight of red ginseng extract, 2 to 7 parts by weight of golden extract, 2 to 7 parts by weight of tomato extract, 2 to 7 parts by weight of 100 parts by weight of the composition. Negative uterine extract, 2-7 parts jasmine extract, 2-7 parts cucumber extract, 2-7 parts pine needle extract, 2-7 parts willow extract, 0.1-1 parts peppermint extract, 2-7 parts It is preferable to include a magnolia flower extract, 2 to 7 parts by weight of lavender extract, 2 to 7 parts by weight of jujube extract, 2 to 7 parts by weight of ginseng extract, and 1 to 3 parts by weight of licorice extract. This is because if the content of each extract, including bamboo vinegar solution is out of the above range, there is a fear that the effects of wrinkle improvement, collagen biosynthesis promotion, whitening, etc. may not be sufficiently achieved.

In addition, the external cosmetic composition of the present invention is prepared through the following process.

1st step: red ginseng, golden, tomato, celery, jasmine, cucumber, pine needle, willow, mint, magnolia flower, lavender, jujube, red ginseng and licorice Grinding or solvent extraction.

Second step: Mix the plants and bamboo vinegar solution obtained in the first step with a certain ratio, put it in a jar with a certain amount of water, and stir 3 to 5 times on a daily basis under a temperature of 30 to 40 ° C. Let it ripen for about a week.

Third step: The liquid (stock solution) and the aged powder are separated separately from the mixture obtained by the second step. At this time, the liquid and powder separated from the aged mixture can be separated by various methods. For example, a hydraulic crimping method, a natural separation method, a rotary dehydration method may be selectively used.

4th step: The aged powder obtained by the said 3rd process is put into a distiller, and finally extracted with distillate gradually at high temperature 200-300 degreeC or more.

In addition, the plant extract included in the composition of the present invention may be in the form of a powder, each plant is extracted using water, an organic solvent, or a mixed solvent thereof, or pulverized with a grinder. In addition, all of these components may be mixed and extracted at the same time, or individual components may be extracted and mixed under optimum conditions. Specifically, the extraction solvent is a C1-4 alcohol, or a mixed solvent of purified water and alcohol, including purified water and methanol, ethanol, propylene glycol, propyl alcohol, butylene glycol, butyl alcohol, glycerin, etc. It is preferable to use single or 2 types or more selected from the group which consists of these. In addition, the extract extracted by hot water or cold extract is filtered to remove suspended solid particles, for example, by filtering the particles using nylon or the like, or using a freeze filtration method, and then used as it is or lyophilized, vacuum Drying, hot air drying, spray drying can be used for drying.

Hereinafter, a general look at the general properties and pharmacological action of the components included in the cosmetic composition for external skin of the present invention.

Bamboo liquor is a purified liquid that is extracted by condensing smoke from the process of burning bamboo or oak charcoal with the main component as acetic acid with a cooling device. The components of bamboo vinegar are 80 ~ 90% of the water, but the components of bamboo vinegar are organic acids that dissolve various substances in biological tissues such as acetic acid, formic acid, propionic acid, lactic acid, iso lactic acid, Many species including valeric acid, crotonic acid, and chigrin acid were included. Phenols that enhance the permeability and absorption in tissues of plants and animals include species of phenol, cresol, and guiacol. It is composed of compounds of more than 200 kinds of trace components, such as compounds, alcohols, neutral components, basic components, etc., and there is a possibility of using them as natural substances. The bamboo vinegar liquid emits strong odor and is used as a disinfectant or smoker, but evaporates in the air in a short time. The bamboo vinegar liquid has a variety of uses. It is widely used as a concentrated raw material such as chemical raw materials, medicine, fertilizer and food additives.

The bamboo vinegar liquor is collected by the carbonization (pyrolysis) of bamboo, and the smoke generated from the charcoal kiln to make bamboo charcoal liquefied while falling in contact with the outside air, the pH is 2.8-3.1 acid solution, the water content is approximately 80 ~ Smoke is collected when the temperature of the charcoal kiln is at 85 to 120 ° C, and the aged moisture content is 60 to 90 wt%, more preferably, 70 wt% of bamboo vinegar for at least 6 months, and the oil is wound up at the top. Purified by removing the tar (Tar) precipitated in the bottom portion.

Red Ginseng ( Panax ginseng ) collects and washes ginseng in September and October, steams, dries, removes thin roots, weighs them, and compresses, dries, inspects and packs them by applying a little steam for compression packing. Manufactured via Steaming and drying process is the most important process to determine the value of red ginseng. Red ginseng contains anti-aging component (Maltol), cancer cell proliferation inhibitory ingredient, anti-tumor ingredient (Ginsenoside Rg ₂ ), cancer cell metastasis inhibiting ingredient (Ginsenoside Rg ₃ ). Red ginseng also contains the largest number of saponins than any other ginseng. Saponin pharmacological action has been shown to have various pharmacological actions such as anti-tumor action, anti-fatigue action, mental stability action, anti-inflammatory action, anti-aging action. Red ginseng is more easily digested and absorbed than ginseng because the starch particles in ginseng tissue are converted from sol to gel. In addition, red ginseng undergoes a steaming process in the manufacturing process, in which the starch particles in the tissue are gelatinized and the tissue is hardened after drying, and various enzymes are inactivated during the steaming process so that the self-extinguishing does not occur. Even if it is stored, there is no change of contents.

Saponin, which is named ginsenoside, the main active ingredient of red ginseng, has a structure of glycosides having a dammarane skeleton of triterpenoids. When combined with organic acids, ginsenosides are effective in improving wrinkles. Many side Rg ₂ or Rg ₃ are produced. The organic acid is contained in a large amount of bamboo vinegar contained in the cosmetic composition of the present invention, the cosmetic composition of the present invention through the combination of the ginsenoside of the organic acid of the bamboo vinegar and red ginseng may have an anti-wrinkle effect.

Golden ( Scutellaria baicalensis ) is a perennial herb belonging to the Lamiaceae family . It is a herb that is frequently used for heat and detoxification. When the roots are removed and the roots are peeled, they are yellow in color. When heat poisoning or chills occur frequently, it is mainly used for the symptoms caused by heat in the lungs, and it is worsened by boils and tumors in the body, like thirst and jaundice, dysentery, diarrhea, indigestion, and nausea caused by heat. Heal back pain and so on.

Tomato ( Solanum Lycopersicum MILL.) Is about 90% water in the fruit and contains vitamins A, B, C, E, K and potassium, calcium, minerals, carotene and lycopene. Beta-carotene and lycopene in tomato are known to have cancer prevention functions, and it is known to play a role in relieving constipation to make elastic and fair skin, and controlling the amount of moisture in the body to suppress overeating, It is known to have a function of promoting digestion and activating the action of the stomach, pancreas, and liver. In addition, the effect of the large intestine is known to be effective in lowering cholesterol levels in the blood and preventing obesity, so eating tomatoes for a long time will make the skin clean and elastic. Tomatoes are known to be particularly effective in preventing vitamins from aging and preventing osteoporosis and senile dementia, especially among vitamin C. These vitamins contain anti-aging ingredients that help to rejuvenate your body, prevent osteoporosis, and vitamin C, which is contained in tomatoes, is more potent in inhibiting carcinogens than vitamin C in other fruits and vegetables. In addition, it promotes metabolism and regulates the amount of urine, and is good for febrile diseases such as hepatitis. In addition, it is described in agreement that the customary skin disease is effective by drinking a little water boiled tomato roots, stems, and leaves, washing the body frequently with this water, and drinking a little foreign body.

The extraction method of the tomato extract is not particularly limited, but the prepared tomatoes are physically pulverized, and the pulverized tomatoes are extracted using a mixed solvent of 1,3-butylene glycol and olive oil. At this time, the mixed solvent is the best extraction of tomatoes in a solvent in which 1,3-butylene glycol and olive oil is mixed in a weight ratio of about 1: 1. However, the weight ratio does not necessarily mean 1: 1, but may also include a mixed solvent in a weight ratio having a slight error.

Cnidium The roots of officinale) is being used in a variety of gynecologic diseases, poor circulation of blood to the target, tonic, sedative and analgesic, such as in Oriental, anemia and menstrual disorders. In particular, it inhibits the activity of melanocyte stimulating hormone, and this property, as a result, inhibits the production of tyrosinase, thereby inhibiting the production of melanin, thereby preventing pigmentation and showing a whitening effect. The uterus used in the cosmetics of the present invention can be obtained using various organs, tissues (for example, roots, leaves, flowers, stems, fruits and seeds, etc.) of the uterus, most preferably from the root of the uterus.

Cucumis sativus ) is a perennial vine plant belonging to the family of cucumbers. Cucumbers contain more than 95% water, a little bit of vitamins and minerals, and a large amount of ascorbinase. It is effective in diuretic effect, gastrointestinal diseases, edema, etc. It is effective to cut the cucumber stem and apply water on the sweat band, which is used as a lotion because it makes the skin fine. In addition, many chlorophylls and vitamin C in cucumbers have a whitening effect that makes the skin white and transparent, and also moisturizes the skin.

Pine needles ( Pinus) The main components of densiflora Siebold et Zuccarini) are ascorbic acid, carotene, vitamin K, B, bitter substance, flavonoid anthocyanin, resin, tannin, etc. Seeds have shikimic acid, and live pine and terebin oil have bactericidal and anti-inflammatory effects and are well absorbed through the skin, so when applied to rheumatoid arthritis, the pain stops and the inflammation heals, and the pollen is hemostatic. , Songhwa powder is used to treat arteriosclerosis, and it has skin regeneration effect due to strong antioxidant effect and sun protection.

The pine needle extract may grow naturally in nature, or any pine needle that is artificially cultivated may be used.

Willow ( Salix) koreensis ) can be used as a medicine to lower blood pressure, reduce fever, and dissolve lacquer. Willow bark contains tannins, flavonoids, ascorbic acid, glycosides, and fats. Salitin has the effect of reducing the heat and pain in the bones. Willow bark water is effective for pneumonia, colds, fever, cough, headache, urine, etc., killing bacteria and removing inflammation, and has a very strong antiseptic effect. Willow bark helps to relieve pain in fever, which can be used for rheumatoid arthritis, colds, and mucus. Flowers are used for jaundice, spear or knife injuries, stiffening and paralysis due to moisture, swelling, burns, poisoning, and soreness of teeth or gums.

Peppermint ( Mentha arvensis var. piperascens is a perennial root of dicotyledons and moss plants. Its main components are alpha-pinene (+,-), anisealdehyde, doucosterol and eugenol. ), Linalool (Linalool), menthone (Menthone), (+)-neomenthol ((+)-Neomenthol), etc., contracting the mucous membranes and blood vessels of the skin, paralyzing nerve peripherals, pain relieving It has the effect of stopping pruritus, giving the skin a unique refreshing feeling, improving skin disease, and having a soothing and antioxidant effect. Preferred sites for use according to the invention are leaves and stems.

Magnolia denudata Desrousseaux is dried flower buds, which are effective for disinfection and pain relief, itching, antiviral, antibacterial and bactericidal effects, lowering blood pressure, sinusitis, headache, toothache, rhinitis It is known to be used for anti-inflammatory. As the components of the god, β-caryophyllene, β-caryophyllene, Terpinen-4-ol, Neolignan Denudatin, Buchellin, Eudemin ( Euduesmin), phylllogenin, citral, Eugenol, 1,8-cineole Lanuginosine, Oxousinsunine and the like.

Herbs have been used mainly in folk medicine, tea and air fresheners. In addition, it has been used in various ways as insect repellents, preservatives, food additives, and recently has been used as a fragrance (such as perfume or cosmetics) using the extract extracted from the herb. Herbs come in various varieties depending on where they grow and the taste, flavor, and composition of the ingredients are different.

The cosmetic composition of the present invention is a herbaceous plant rosemary, lavender, thyme, sage, spearmint, lemongrass, rosehip, echinacea, jacque, sweet fennel, mate, yukari, ginger, chamomile, stevia, jasmine, lilac, orange For example, a flower, an eye bright, an orchid, a mauve, and the like, but is not limited thereto, and may be selected by arbitrarily combining two or more herbal plants used in cosmetics. In particular, it is preferable to use jasmine and lavender.

Jujube ( Ziziphus jujuba ) is sweet and a little warm, the action site is rain, stomach, heart, liver. Proteins, sugars, organic acid mucus and vitamins (A, B2, C), etc. are included. Serum total protein and albumin (Albumin) increases and protects the liver.

Sophorae Radix contains alkaloids such as d-matrin, d-oxymatrine, d-sophoranol, l-methyl cyticin, l-baptoline, and l-sopocarpine. Xanthohumol, isoxanthohumol and camphorol are contained. Main effects include diuretic and antibacterial effects. It is harvested mainly in spring and autumn.

Licorice ( Glycyrrhizae Radix ) contains 3.63 ~ 13.06% of glycyrrhizic acid, triterpenoid saponin, and various other flavone compounds. As a botanical medicine, it protects the lungs and the nasal cavities to prevent the blood circulation of the face from disturbing due to the external turbidity and to remove the moisture inside and remove the skin inside and outside. It works to make you healthy.

Cosmetic compositions of the present invention can be prepared in any formulation conventionally prepared in the art, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing , Oils, powder foundations, emulsion foundations, wax foundations and sprays, and the like, but are not limited thereto. More specifically, it may be prepared in the form of a flexible lotion, nutrition lotion, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder.

In addition, in the cosmetic composition of each formulation, other components in addition to the extracts can be suitably selected by those skilled in the art without difficulty according to the formulation or purpose of use of other cosmetics. For example, fats and oils, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, flavorings, blood circulation accelerators, Cooling agent, a restriction | limiting agent, purified water, a carrier, etc. are mentioned.

When the formulation of the present invention is a paste, cream or gel, animal oils, vegetable oils, waxes, paraffins, starches, trachants, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide may be used as carrier components. Can be.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular in the case of a spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystals Soluble cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, an isethionate, an imidazolinium derivative, a methyltaurate, a sarcosinate, a fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

Hereinafter, the present invention will be described in detail by way of examples. However, the following examples are merely to illustrate the invention, but the content of the present invention is not limited to the following examples.

Example 1 Preparation of Cosmetic Composition

Golden roots, celestial roots, jasmine leaves, stems and flowers, cucumbers, pine needles, willow leaves and annual stems, mint leaves and stems, magnolia buds, lavender leaves, stems and flowers, jujube, red ginseng roots and Each licorice was washed, dried, and ground to a powder state of 50 mesh or more using a grinder.

The red ginseng was selected from high quality raw materials, and put into the extraction tank, followed by extraction three times at 70 ° C. for 11 hours using 70% or more ethanol. The amount of the extraction solvent was 6 times the input amount, and the filtrate was obtained using a 100 µm filter, and then concentrated under reduced pressure at 60 ° C under 600 mm Hg. The concentrate was sterilized at 80 ° C. for 2 hours, and quantitatively filled in a sterilized container to obtain a red ginseng extract.

The tomatoes are physically pulverized in a general grinder to make 50 g of a pulverized powder, and then extracted for 48 hours using a mixed solvent in which 1,3-butylene glycol and olive oil are mixed at a weight ratio of about 1: 1 (25 g each). And filtered to 100 mesh.

The bamboo vinegar solution was indirectly heated while putting 1,000 kg of bamboo in a cauldron and kept at 80 ° C. The sap vapor contained in the smoke of the stack was collected and cooled at a temperature of 90 ° C, and condensed. Prepared by aging and separation at 30 ° C. for 6 months. The bamboo vinegar liquor was once filtered through a filter having a pore size of 5.0 μm, and a purified product having no tar component was used.

Golden 50g, Cheongung 50g, Jasmine 50g, Cucumber 50g, Pine needle 50g, Willow 50g, Peppermint 5g, Magnolia bud 50g, Lavender 50g, Jujube 50g, Red ginseng 50g and Licorice 20g Powder, Red ginseng extract 50g, Tomato extract 50g And 50 g of bamboo vinegar solution were mixed. Two times the volume of purified water was added to the mixture and aged for about one week in a natural atmosphere with stirring evenly four times on a daily basis under a temperature of 35 ℃.

The liquid and the aged powder were separated separately from the mixture by the maturing using hydraulic pressing.

The aged powder was put into a still, and finally extracted with distillate at a high temperature of 250 ° C., which was used as a raw material for cosmetics.

Experimental Example 1 Wrinkle Improvement Effect

In order to determine the anti-wrinkle effect of the composition prepared in Example 1, the skin cell (skin cell) proliferation effect was examined.

To this end, human normal fibroblasts (Human Normal Fibroblast) were inoculated to 1 × 10 ⁴ cells in each well of a 96-well microplate and incubated in DMEM (Dulbecco's Modified Eagle's Medium) medium for 24 hours. After culturing, the composition of Example 1 prepared above and each extract were replaced with a serum-free DMEM medium adjusted to a final concentration of 250 μl / mL, followed by further incubation for 24 hours. 3- (4,5-dimethylriazol-2-yl) -2,5-diphenyltetrazolium (MTT: 3- (4,5-dimethyl-thiazol-2-yl) 2,5-diphenyltetrazolium: 5 mg / 10 mL) was added and the mixture was left for 4 hours, and then the medium portion was discarded. 100 μl of dimethyl sulfoxide (DMSO) solution was added to each well, and the absorbance was measured at 570 nm with a microplate reader. Performing the above procedure to obtain the cell proliferation effect from the following formula and the results are shown in Table 1

Cell proliferation effect (%) = {(absorbance at sample treatment-absorbance of control) / absorbance of control group} × 100

Cell proliferation effect Sample (500 µg / mL) Cell proliferation effect (%) Control - Compositions of the Invention 90.7 Bamboo liquor 75.1 Red ginseng 51.2 Gold 50.0 tomato 61.3 Cheongung 73.0 Jasmine 52.3 cucumber 33.7 pine needles 32.5 Bud tree 44.3 mint 53.6 Magnolia flower 42.6 lavender 53.6 Jujube 53.5 Gosam 45.9 licorice 32.6

As shown in Table 1, the composition of the present invention can be seen that the cell proliferation effect is excellent in human normal fibroblasts than when treated with each extract alone.

Experimental Example 2 Collagen Biosynthesis Effect

Collagen is a biomaterial that accounts for most of the skin's connective tissue. It is related to skin elasticity and decreases with age. Therefore, increasing the amount of collagen biosynthesis can increase the elasticity of the skin. Thus, in The effect of promoting collagen biosynthesis was examined by in vitro method.

Specifically, human normal fibroblasts (Human Normal Fibroblast) were seeded (1 × 10 ⁵ per well) in 24-wells and cultured until they developed to about 90%. This was washed once with PBS, and then treated with the composition of Example 1 prepared above and each extract (diluted to 1 × 10 ^-6 ) and incubated in a CO ₂ incubator for 24 hours. The supernatant was removed and the amount of procollagen type (I) C-Peptide (PICP) was increased by using an ELISA kit. Synthetic capacity is prepared by the control (non-treated group) 100.

Collagen biosynthesis promoting effect Sample (500 µg / mL) Collagen Synthesis (%) Control 100 Compositions of the Invention 190 Bamboo liquor 121 Red ginseng 145 Gold 150 tomato 162 Cheongung 154 Jasmine 160 cucumber 135 pine needles 110 Bud tree 136 mint 125 Magnolia flower 156 lavender 155 Jujube 160 Gosam 120 licorice 115

As shown in Table 2, it can be seen that the composition of the present invention is superior to the collagen biosynthesis promoting effect than the treatment of each extract alone.

Experimental Example 3 Measurement of Tyrosinase Activity Inhibition

Enzyme tyrosinase used human tyrosinase. The activity of the enzyme was measured at 475 nm to produce DOPA chromium, the browning product of L-DOPA. 760 μl of 50 mM Tris-HCl buffer solution (pH 7.5) was added to the test tube, and maintained at 37 ° C., and 190 μl of 20 mM LDOPA, 50 μl of enzyme solution and 40 μl of the target for whitening activity were mixed, and the absorbance change was measured at 475 nm. At this time, the measurement was performed without putting the target sample as a control. When the enzyme activity of the control group was seen as 100, each enzyme inhibitory activity was expressed in% compared to the control group. At this time, in order to observe the relative strength of the tyrosinase inhibitory activity of the composition of Example 1, the relative activity with arbutin widely used in the whitening cosmetics was compared.

Tyrosinase inhibitory activity (%) by the target sample = (absorbance at the time of sample addition / absorbance of the control group) x 100

Tyrosinase inhibitory activity sample Inhibitory Activity (%) Compositions of the Invention 89 Bamboo liquor 60 Red ginseng 45 Gold 38 tomato 49 Cheongung 51 Jasmine 42 cucumber 48 pine needles 40 Bud tree 25 mint 30 Magnolia flower 27 lavender 42 Jujube 45 Gosam 28 licorice 32 Comparative substance 35

As shown in Table 3, the composition of the present invention showed a significantly higher effect on human tyrosinase activity.

Experimental Example 4 Inhibitory Effect on Melanin Synthesis

B-16 / F10 melanoma cells were plated in 6-well plates with a cell number of 2 × 10 ⁴ and incubated in a 37 ° C., 5% CO ₂ incubator for 24 hours. After incubation, the medium was removed and treated with DMEM medium containing 10% FBS, 2mm theophylline as a fresh medium, and the test sample was added every day for 3 days. Cells were harvested by removing the culture solution and treated with 0.25% trypsin-EDTA solution. After centrifugation, the supernatant was removed, and 100 µl of 1N NaOH was added to dissolve intracellular melanin. The solution was diluted with PBS, and then absorbance was measured at 475 nm with an ELISA reader to determine the melanin inhibition rate of the sample. Blank used a culture of B-16 / F10 melanoma cells without processing the sample.

Melanin production inhibition rate (%) = 100- (absorbance of sample / absorbance of control × 100)

Melanin production inhibitory effect sample Melanin production inhibition rate (%) Compositions of the Invention 70 Bamboo liquor 41 Red ginseng 38 Gold 46 tomato 48 Cheongung 45 Jasmine 25 cucumber 38 pine needles 54 Bud tree 25 mint 26 Magnolia flower 19 lavender 26 Jujube 32 Gosam 38 licorice 35 Comparative substance (arbutin) 21

As shown in Table 4, it can be seen that the whitening effect of the present invention is excellent.

Experimental Example 5 Whitening Effect Test on Human Skin

Twenty healthy men were attached with an opaque tape with six 1.5cm diameter holes in the upper forearm of the subject, and then irradiated with UVB (1.5-2 times the minimum erythema) of each subject. After induction of two months after the test materials were applied to measure the contrast of the skin using a color difference meter. Each sample was dissolved in a vehicle of propylene glycol / ethanol (8/2) at a concentration of 1% and corrected twice daily (morning and evening).

The contrast of the skin is expressed as the 'L' value and ranges from 0 (black) to 100 (pure white) (Koreans generally have values between 50 and 70).

When the sample was applied, the L value gradually increased when there was an effect, and the comparison between the samples was expressed as ΔL (final L value—the value immediately before applying the sample).

Whitening effect on human skin sample ΔL Compositions of the Invention 3 Bamboo liquor 1.5 Red ginseng 1.6 Gold 1.8 tomato 1.3 Cheongung 1.2 Jasmine 2.0 cucumber 1.9 pine needles 2.1 Bud tree 1.3 mint 1.2 Magnolia flower 1.2 lavender 2.5 Jujube 2.3 Gosam 2.2 licorice 2.0 Comparative substance 1.2

As shown in Table 5, the composition of the present invention showed a very high melanin production inhibitory effect was shown to be superior to the arbutin already known to exhibit an excellent whitening effect. In addition, it was found that the synergistic effect was significantly higher than that of each extract alone.

Experimental Example 6 Skin Irritant Effect Test

The skin safety of the composition of Example 1 was compared. Normal patch test was conducted to check the safety of the skin. After the pinchamber containing the sample was placed on the inner part of the arm of 18 people, after 24 hours and 48 hours, the patch was removed and the skin condition was observed. The average value was calculated and the results are shown in Table 6.

(Score)

4: very irritating (skin damaged); 3: severe irritation (vesicles, etc.) 2: slight irritation (redness); 1: almost no irritation; 0: No irritation at all, good for sensitive skin.

Sample Name Judgment 24 hours later 48 hours later Compositions of the Invention 1.0 0.5

 As shown in Table 6, the composition of the present invention can be seen that there is little skin irritation can be evaluated as suitable for use as a raw material for cosmetics.

Hereinafter, a cosmetic preparation example using the composition of the present invention is shown. That is, lotion, emulsion and cosmetic liquid containing the composition obtained in Example 1 were prepared in Preparation Examples 1 to 3. The lotion, emulsion and cosmetic liquid containing the composition of Example 1 showed an excellent effect on skin improvement such as collagen synthesis promoting effect, skin fine wrinkle improvement effect and whitening effect. The composition of Example 1 may be added to the formulation of the lotion, gel, water-soluble liquid, cream, essence, oil-in-water (O / W) type and water-in-oil (W / O) type, which can be prepared by a person skilled in the art It can be easily applied in the art, but the content of the present invention is not limited thereto.

Preparation Example 1 Preparation of Lotion Containing Composition of the Present Invention

To 8 g of 95% ethanol, 0.05 g of polypyrrolidone, 0.1 g of oleyl alcohol, 0.2 g of polyoxyethylene monooleate, 0.2 g of fragrance, 0.1 g of paraoxybenzoic acid methyl ester, a small amount of antioxidant, and a small amount of pigment were dissolved. . 0.05 g of the composition obtained in Example 1 and 5 g of glycerin were dissolved in 85.33 g of purified water, and then the mixed solution was added and stirred to obtain a lotion having a skin improvement effect.

Preparation Example 2 Preparation of Milky Way Containing Composition of the Present Invention

1.2 g of cetyl alcohol, 10 g of squalane, 2 g of petrolatum, 0.2 g of paraoxybenzoic acid ethyl ester, 1 g of glycerin monoesteralide, 1 g of polyoxyethylene (20 M addition) monooleate and 0.1 g of fragrance were dissolved by heating at 70 ° C, 0.5 g of the composition obtained in Example 1, 5 g of dipropylene glycol, 2 g of polyethylene glycol-1500, 0.2 g of triethanolamine, and 76.2 g of purified water were heated and dissolved at 75 DEG C. O / W) type skin emulsion having an effect of improving skin was obtained.

Preparation Example 3 Preparation of Cosmetic Liquid Containing Composition of the Present Invention

5 g of 95% ethyl alcohol, 1.2 g of polyoxyethylene sorbitan monooleate, 0.3 g of kitulose, 0.2 g of sodium hyaluronate, 0.2 g of vitamin E-acetate, 0.2 g of sodium licorice, 0.1 g of paraoxybenzoic acid ethyl ester, 1 g of the composition obtained in Example 1 and a suitable amount of the pigment were mixed to obtain a cosmetic solution having an effect of improving skin.

Claims (7)

Contains bamboo vinegar, red ginseng extract, golden extract, tomato extract, celery extract, jasmine extract, cucumber extract, pine needle extract, willow extract, peppermint extract, magnolia extract, lavender extract, jujube extract, high ginseng extract and licorice extract as active ingredients Cosmetic composition for external skin. The method of claim 1, The extract is an external skin cosmetic composition, characterized in that extracted with water, lower alcohol having 1 to 4 carbon atoms or a mixed solvent thereof. The method of claim 1, 2 to 7 parts by weight of bamboo vinegar, 2 to 7 parts by weight of red ginseng extract, 2 to 7 parts by weight of golden extract, 2 to 7 parts by weight of tomato extract, 2 to 7 parts by weight of uterine extract, 2 to 7 weights Jasmine extract, 2-7 parts by weight cucumber extract, 2-7 parts by weight pine needle extract, 2-7 parts by weight willow extract, 0.1-1 parts by weight mint extract, 2-7 parts by weight magnolia extract, 2-7 weight Lavender extract, 2 to 7 parts by weight of jujube extract, 2 to 7 parts by weight of ginseng extract and 1 to 3 parts by weight licorice extract comprising a cosmetic composition for external skin. The method of claim 1, Having a formulation selected from the group consisting of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations and sprays Cosmetic composition for external skin. The method of claim 1, Oils and fats, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, UV absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, flavorings, blood stimulants, cooling agents, restriction A cosmetic composition for external application of skin, further comprising at least one additive selected from the group consisting of an agent, purified water and a carrier. The method of claim 1, Cosmetic composition for external skin, characterized in that the wrinkles improve effect and collagen biosynthesis effect to increase the skin vitality. The method of claim 1, Cosmetic composition for external skin having the effect of inhibiting melanin synthesis to brighten the skin color.